LOCUS       NZ_CP055282             5594 bp    DNA     circular CON 03-MAY-2024
DEFINITION  Acinetobacter sp. NEB 394 plasmid pBspH14, complete sequence.
ACCESSION   NZ_CP055282
VERSION     NZ_CP055282.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15195663
            Assembly: GCF_013374795.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter sp. NEB 394
  ORGANISM  Acinetobacter sp. NEB 394
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 5594)
  AUTHORS   Morgan,R.D.
  TITLE     Acinetobacter species H NEB394 complete genome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5594)
  AUTHORS   Morgan,R.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUN-2020) Genome Biology, New England Biolabs, Inc.,
            240 County Road, Ipswich, MA 01938, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: FEB-2018
            Assembly Method                   :: HGAP v. 3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 217.0x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013374795.1-RS_2024_05_03
            Annotation Date                   :: 05/03/2024 04:29:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,807
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,431
            CDSs (with protein)               :: 3,431
            Genes (RNA)                       :: 113
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 84 of 263
            Pseudo Genes (incomplete)         :: 172 of 263
            Pseudo Genes (internal stop)      :: 55 of 263
            Pseudo Genes (multiple problems)  :: 43 of 263
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP055282.1.
            Bacteria and source DNA available from Richard Morgan, New England
            Biolabs, Ipswich MA 01938.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5594
                     /organism="Acinetobacter sp. NEB 394"
                     /mol_type="genomic DNA"
                     /strain="NEB 394"
                     /isolation_source="soil"
                     /db_xref="taxon:2743575"
                     /plasmid="pBspH14"
                     /geo_loc_name="USA:Massachusetts"
                     /collection_date="1986"
                     /collected_by="Daniel Hall"
                     /note="This strain was originally classified as a Bacillus
                     species, and is the source of the commercial restriction
                     endonuclease BspHI; however from the sequence data it is
                     clearly an Acinetobacter, not a Bacillus so it was
                     submitted as Acinetobacter species H (NEB394), formerly
                     known as Bacillus species H (NEB394)."
     gene            join(3755..5594,1..116)
                     /locus_tag="HUK62_RS17135"
                     /old_locus_tag="HUK62_17135"
     CDS             join(3755..5594,1..116)
                     /locus_tag="HUK62_RS17135"
                     /old_locus_tag="HUK62_17135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017389689.1"
                     /GO_function="GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="WP_004870594.1"
                     /translation="MKVKFLTVSLMFILPFSVFAANGQVEKAVIESNNAYKTKYNLDDM
                     TEFNYAQKGFIAKPTGQIKSEKGNVIWDFDAFNFLNDPAPNTVNPALWRQAKLNNNVGL
                     FKVTDRVWQIRGFDLANMTIIQGDTGWIIVDTLTSKETAEAALKFARQHLGEQKISAII
                     FTHSHIDHFGGALGVLSTEEINARKTPIIAPQGFMEEATSENLMAGSAMIRRATYMYGT
                     FLPKNAEGLVDTGLGKAVAVGKMGILEPTQLITQAEQKMTIDGLDFVFYNVPGSEAPAE
                     LTFSIPSLKLYNGAEILSHTMHNLYTLRGAKVRDALKWVGYLDQAMQHAKASDVLIAQH
                     HWPVWGNDNIQDFIKTQRDVYKFTHDQTVRYMNSGFNGAEIAEKIQLPAALDQKLYAHG
                     YYGTLKHNVKAIYQYYMGWFDAHPSNLDPLPPKAVAKKYIELAGGENNALKNARDAYAQ
                     ADYRWAAEILKHIVLNNPQNQQAKDLLANTYRQLGYAAEASTWRNFFLVGAQELQNNVP
                     LQNTSDPSDLLIHTPTERFLEAMATNLDVENLKNENQCINLVLSDTQENFSLWVENSIM
                     QFKRHDDSKDLASDCPTLTVTKPLYLKMITGQIKGVKVLLSNESKVKGNPLEIGKFFAM
                     FKRPDSTFPIVTRPND"
                     /name="alkyl sulfatase dimerization domain-containing
                     protein"
     gene            complement(349..2163)
                     /locus_tag="HUK62_RS17140"
                     /old_locus_tag="HUK62_17140"
     CDS             complement(349..2163)
                     /locus_tag="HUK62_RS17140"
                     /old_locus_tag="HUK62_17140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004706791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_004706791.1"
                     /translation="MIVDFFRHGSGLSKGCLDYLLGEDREREHAQVLSGDVDLTAQLID
                     SSPFAKKYTSGCLSFYEHALSDQDKQQIMQNFEECLFPGLDKDQYQILWVQHQDKVNQD
                     TGETRLELNFVIPNVELSTGKRLQPFYAPVDLDRVDLFKQITNAEHQLYDPDDPNNQQL
                     LINKKNLPKDVKDFKDQLHQRIYQAVVDGKVADRQKLVEWLESNEIKVTRQTQKSISIE
                     NPYEGATRPIRLSGEVYEQGFSATGEYRQEVQQRIATYRGTTSERYRANVTDYQRQLEH
                     KSQYHSDRYPAVRREISAEPTEQLTADREAARPVPSLATVQIEPFKANERADTDKRTAS
                     SEQKPTETAYHFEYGNDFGSCYFNYSHYCARFYGQKQVQRDTDTQYDHQRETNASGSPE
                     PIGGQFDVQHMQTAKQQSPTSMYSDQQGRRGLAEQLHGADGVLSDDRIRNTVIENHRRT
                     TAAITATTNSVAAATAEFRRDAKENYPSLISTIKGNGERTDRLSANTKVIIDNTDTISR
                     IGKKYSDLYRADQWQSQGDEPNYNELRGRTGENLNHTTVSLVTSSQSSAEFARNVRTIE
                     ANIVQMKERKKEMEQPQPKRDRGMNFGM"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2153..2554)
                     /locus_tag="HUK62_RS17145"
                     /old_locus_tag="HUK62_17145"
     CDS             complement(2153..2554)
                     /locus_tag="HUK62_RS17145"
                     /old_locus_tag="HUK62_17145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004706792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_004706792.1"
                     /translation="MEIDSVTKQKVVRERTFKVRLSESEYQLLDANNPFGSIAKLLRQS
                     AVDKATKIYNSKADDAEKIKSTKIVESSYSKTERNLILELGRIGNNVNQIAKAINTDIA
                     GTGTFDKVKLLHLLISIDQQLRELRPDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(353..2163)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tcctgctttc taacgaaagt aaagtcaaag gcaatccatt ggaaattgga aaattctttg
       61 caatgtttaa acgtccagac tcaacttttc caattgtcac tcgaccaaat gattaggatg
      121 aaatgactgg gcttaaagaa ccctaccaaa ttaaagtctc cgcttttatg tgcacacatt
      181 agtgcacata aatacaaaat tagtttaatt atttcaataa tttacaatca ataaaaaagc
      241 ctaacttgtc taagtcgggc ttttttattg attcttcacg tttcacgcct aatatttaat
      301 cattgttcca tatttttgat tagcaacagt cggaatcgag cataaaccct acatgccaaa
      361 attcatgccc cgatctcgct taggttgggg ctgctccatt tcctttttcc gttccttcat
      421 ttggacgata ttcgcttcaa tagttcggac gtttcgagca aattctgctg agctttggct
      481 acttgtgacg agactgaccg ttgtgtggtt gagattttct ccagtacgtc ctcgtaattc
      541 gttgtaattt ggctcatctc cctgactttg ccattgatct gctcggtata ggtcgctgta
      601 tttttttcca attcgtgaaa ttgtgtctgt attgtcgata atgacttttg tgtttgcact
      661 aagtctgtct gtacgctctc cattgccttt gattgtgctg ataaggcttg gataattttc
      721 ttttgcatca cgtctaaact ctgctgttgc tgctgcaact gagtttgttg ttgctgtaat
      781 tgctgctgtt gttcttcgat gattttcaat aactgtgttt ctaattcggt catcacttaa
      841 taccccatcg gcaccatgaa gttgttctgc caatcccctg cgtccttgtt gatctgaata
      901 catagacgtt gggctttgct gttttgctgt ctgcatgtgc tgaacgtcaa actgcccccc
      961 gatcggttca ggtgatccac tcgcatttgt ttctcgttga tggtcatatt gagtgtccgt
     1021 atctcgttgt acttgctttt gcccataaaa ccgagcgcaa taatgagaat aattaaaata
     1081 gcagctccca aaatcattgc catattcaaa gtggtaagct gtttcagtag gcttttgttc
     1141 tgaacttgct gttcttttgt ctgtatctgc tcgttcattt gctttaaacg gctcaatttg
     1201 gactgtagca aggcttggta ctggtcgagc tgcttctcga tctgctgtga gctgttctgt
     1261 aggttctgcg ctaatttctc gtctgactgc tggatagcgg tcgctgtgat attggctctt
     1321 gtgctcaagc tgtctttgat aatctgtgac gtttgctcga taccgctcag aagttgttcc
     1381 tcggtacgtt gcaattcgct gttgtacttc ttgtcgatat tcgccagtag cgctaaagcc
     1441 ttgctcataa acctctccac ttaaacggat aggacgtgtt gcgccctcgt atggattttc
     1501 gattgatatt gatttttggg tttgtcgggt gactttgatt tcattcgatt caagccattc
     1561 aaccaatttt tgccgatcgg caactttgcc atcgactaca gcttggtaaa tcctttggtg
     1621 taactgatct ttaaaatcct tcacatcctt gggtaagttc ttcttattga ttaaaagctg
     1681 ctgattgttc ggatcatcgg gatcatatag ttgatgttct gcattggtaa tctgcttaaa
     1741 cagatccact cgatctaaat ccactggcgc ataaaatggc tgtagccgtt tgcctgtcga
     1801 aagctcaacg tttggaatca caaaattcag ctcaagccgt gtctctccag tatcttgatt
     1861 caccttatct tggtgctgta cccataaaat ttggtactga tctttatcga gaccagggaa
     1921 aaggcattcc tcaaaatttt gcatgatctg ctgtttatcc tggtcagata aagcatgctc
     1981 gtaaaaggat aaacaaccac tggtatattt cttggcaaaa ggactgctat caatcagttg
     2041 agcggtcaag tcaacatcac cacttaaaac ctgtgcatgt tctcgctctc tatcttcacc
     2101 aagcaaataa tctagacaac ctttagataa tcctgaacca tgtctaaaaa aatcaacgat
     2161 catctggtcg taactcccga agttgttgat ctatggaaat caacaaatga agtaacttca
     2221 ctttgtcaaa agtacctgtc cctgcaatat ccgtattaat cgctttggca atctgattca
     2281 cgttattacc tatacgaccc aattctagga ttaaatttct ctctgtcttg ctatatgaag
     2341 attcaacaat ctttgttgat ttaatttttt cagcatcatc tgctttggaa ttataaatct
     2401 ttgttgcttt atctactgct gactgacgta gcaactttgc gattgaacca aaaggattat
     2461 tggcatcgag caattgatat tctgattctg aaagtctgac tttaaacgtc cgttcccgta
     2521 ctactttctg tttagttact gaatctattt ccacttgaac aatcccattc ttggtctaac
     2581 cggtttagag gaaaatcttt gatttttctt taaaccggtt ttcgggggta tcgggggtga
     2641 agcccctgat caaggtcaca gcttagcact attaaacgaa gtgaaatagg ggtaactgtg
     2701 tatccttgct tatttaaaaa gggaaagaat tatttatact aaaatttaat caaatgatac
     2761 agtatataaa ccctaataag aactggcata aatcttgtaa aacaatagca ctcaactctt
     2821 tctcaccacc accacagccc tcgctttgct caggcagatg gtgagcgttg agagagtttc
     2881 ggcaatactc tccctaaggg ctgtcattca aaaaataaca catcccctaa gtattgccga
     2941 tttaacctga ttaggctgac ggagccacgc atcggtcgta tccgcaccat gcttcacata
     3001 catggcagac aaggactaaa ggtactgcac ctctccccac gcgctccctg tcgaagtatg
     3061 gcggtatctc tgcgaggatt tctccaacat gcaacttaac caacctatac aactactctc
     3121 aacgctccgc ttttgtggga ttcccctgta acggttcagc tcctgcttct cagctttcac
     3181 tatcgctacg atgcaccagt gacacttatt ccctccacat gcaccgcata tcttccttaa
     3241 aaaggatgca cagaagctga taaggctcga atactgtcta tgtattgctt attggatgaa
     3301 cgagagtcag gtaacgaaag actttttact gatacaatgc ttgccatttt ttatcaattt
     3361 gatagaattt gaaaagcggt acaagccgaa aggtgaaatc ataacggtcg tcaaacctta
     3421 tgatcttcgt tacttgatgc tcgaacatca aataactgcg taccaccaaa tttaagacaa
     3481 aacccctcta agttgcaaga ctcagagggg tttttcttta ggttttaaca aaatttttat
     3541 ttttctctcc tactaactca tgcccaataa ggactgcgta gttttaataa aaacaggtat
     3601 attcttgtaa aatattattt tatgattcca tcataaaaaa tgatggctgt tattataatg
     3661 tttgaattga aaagcttcta ttaattcaac acatatcaaa aatagtatag acaaggaaat
     3721 caacatttat ggatattttg aagaaggaaa gacaatgaaa gttaaatttt taacggtttc
     3781 attgatgttt attttgccat tttctgtttt tgcagcaaat ggacaagtcg aaaaagcggt
     3841 cattgaatca aataatgcgt ataaaacgaa atataattta gacgatatga cagagtttaa
     3901 ttatgcgcaa aaaggcttta ttgccaagcc aacaggtcaa attaaaagtg aaaaaggcaa
     3961 tgtgatctgg gattttgatg cgtttaattt tttaaatgat ccagcaccaa atactgttaa
     4021 tcctgcacta tggcgacaag caaagctcaa caataatgtt ggtctattta aagtgacaga
     4081 tcgagtttgg caaattcgtg gttttgattt ggcaaatatg accattattc agggtgatac
     4141 tggctggatt attgtggata cattaacctc taaagaaacg gctgaagcag cacttaaatt
     4201 tgctaggcag catttaggtg agcaaaaaat ttcagccatt atctttaccc atagccatat
     4261 cgatcatttt ggtggtgcat taggcgttct ttctacagaa gaaattaacg ctagaaaaac
     4321 cccaattatt gcgccacaag gctttatgga agaagcgacc agtgaaaatt tgatggcagg
     4381 ttcagccatg atacgtcgtg cgacctatat gtatggaacc tttttaccaa agaatgctga
     4441 aggattggtg gatacaggtt taggtaaggc tgttgctgtt gggaaaatgg gaatccttga
     4501 gcctacacaa ctgatcaccc aagcagaaca gaaaatgacc attgatggtt tggattttgt
     4561 gttttataac gtgccgggtt cagaagcgcc agcagagtta accttctcta tcccatcact
     4621 gaaactttat aatggcgcag aaattctttc gcataccatg cataatcttt ataccctccg
     4681 cggtgccaaa gtccgtgatg ccttaaaatg ggtaggctat ttagaccaag caatgcagca
     4741 tgccaaagca tccgatgtat tgattgcaca acatcattgg cctgtctggg gcaatgacaa
     4801 tattcaagat tttattaaaa cccagcgaga tgtttataag ttcacacatg atcagacggt
     4861 gagatatatg aactctggtt ttaatggtgc tgaaattgcc gagaaaattc agcttccagc
     4921 ggcacttgat caaaagctat atgcgcatgg ctattatgga accttaaagc ataatgtaaa
     4981 agcgatatac caatactata tgggatggtt cgatgctcat ccttccaatt tagatccatt
     5041 accgcctaaa gctgttgcaa aaaaatatat tgagttggca ggtggggaga ataatgcatt
     5101 aaaaaatgcg cgggatgcct atgctcaagc tgactataga tgggctgccg agattttaaa
     5161 acatattgtg ttgaataacc ctcaaaacca acaagcaaaa gacttattgg caaatacgta
     5221 tcgtcaattg ggctatgctg ctgaggcttc aacttggcgt aatttctttt tggtgggtgc
     5281 tcaagagcta caaaataatg tacccctaca aaatacatct gatccgagtg atttattgat
     5341 tcacacgcca actgaaagat ttcttgaagc catggcgaca aatttagatg tggaaaattt
     5401 aaaaaatgaa aaccagtgta tcaatctggt tttgtcggat acccaagaga atttttcatt
     5461 atgggttgaa aactcaatca tgcagtttaa gcgtcatgat gacagtaaag atttggcatc
     5521 ggattgccca acattaaccg taaccaagcc cttatatcta aaaatgatta caggtcaaat
     5581 aaaaggggta aaag
//
