LOCUS       NZ_CP055281             6257 bp    DNA     circular CON 03-MAY-2024
DEFINITION  Acinetobacter sp. NEB 394 plasmid pBspH13, complete sequence.
ACCESSION   NZ_CP055281
VERSION     NZ_CP055281.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15195663
            Assembly: GCF_013374795.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter sp. NEB 394
  ORGANISM  Acinetobacter sp. NEB 394
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 6257)
  AUTHORS   Morgan,R.D.
  TITLE     Acinetobacter species H NEB394 complete genome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6257)
  AUTHORS   Morgan,R.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUN-2020) Genome Biology, New England Biolabs, Inc.,
            240 County Road, Ipswich, MA 01938, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: FEB-2018
            Assembly Method                   :: HGAP v. 3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 217.0x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013374795.1-RS_2024_05_03
            Annotation Date                   :: 05/03/2024 04:29:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,807
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,431
            CDSs (with protein)               :: 3,431
            Genes (RNA)                       :: 113
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 84 of 263
            Pseudo Genes (incomplete)         :: 172 of 263
            Pseudo Genes (internal stop)      :: 55 of 263
            Pseudo Genes (multiple problems)  :: 43 of 263
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP055281.1.
            Bacteria and source DNA available from Richard Morgan, New England
            Biolabs, Ipswich MA 01938.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6257
                     /organism="Acinetobacter sp. NEB 394"
                     /mol_type="genomic DNA"
                     /strain="NEB 394"
                     /isolation_source="soil"
                     /db_xref="taxon:2743575"
                     /plasmid="pBspH13"
                     /geo_loc_name="USA:Massachusetts"
                     /collection_date="1986"
                     /collected_by="Daniel Hall"
                     /note="This strain was originally classified as a Bacillus
                     species, and is the source of the commercial restriction
                     endonuclease BspHI; however from the sequence data it is
                     clearly an Acinetobacter, not a Bacillus so it was
                     submitted as Acinetobacter species H (NEB394), formerly
                     known as Bacillus species H (NEB394)."
     gene            complement(9..290)
                     /locus_tag="HUK62_RS17090"
                     /old_locus_tag="HUK62_17090"
     CDS             complement(9..290)
                     /locus_tag="HUK62_RS17090"
                     /old_locus_tag="HUK62_17090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001129137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family toxin"
                     /protein_id="WP_001129137.1"
                     /translation="MAKRKIIVTSSFKRDIKRRYLELVTAEWAEVLDCLVANNPLPEKY
                     LDHPLKGGAEFKNCRDCHVKPDLVLIYRLVGDDILELHQLDSHSEIFG"
                     /name="type II toxin-antitoxin system YafQ family toxin"
     gene            complement(283..576)
                     /locus_tag="HUK62_RS17095"
                     /old_locus_tag="HUK62_17095"
     CDS             complement(283..576)
                     /locus_tag="HUK62_RS17095"
                     /old_locus_tag="HUK62_17095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004973743.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="WP_001277151.1"
                     /translation="MSAVNFNMRLEAELKEQVTPILEDYGLTMPQAFKLFLNQIVKTKA
                     IPLSFDYARETALTPKAAAKLLQSLKEIENGEYTEYETVEEAIKAMSEEAHG"
                     /name="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
     gene            complement(769..1527)
                     /locus_tag="HUK62_RS17100"
                     /old_locus_tag="HUK62_17100"
     CDS             complement(769..1527)
                     /locus_tag="HUK62_RS17100"
                     /old_locus_tag="HUK62_17100"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein"
                     /protein_id="WP_176538532.1"
                     /translation="MYNPHNNYREQTLMKRLLLVTAISFSSLTYADSGFYTSLKAGISD
                     TKFEDYKLTLAQGDESIIFKHKDADESIYPNISAAIGFDFSKVSKVNARAELEYTYKDK
                     ATFAPNISSGIFNGNEEQAPPNIPSYVVNELRTQSLMLNGYYDFKNTTKFTPYLSAGLG
                     VSRIENDISTNPEMFNSSEHISTDTNNNFAWTAGAGVAYQVTKDVALDLAYRYVDAGEI
                     ETSLSFDGANFKNTADLISHDYSLGIRYNF"
                     /name="outer membrane protein"
     gene            2606..3589
                     /gene="repM"
                     /locus_tag="HUK62_RS17105"
                     /old_locus_tag="HUK62_17105"
     CDS             2606..3589
                     /gene="repM"
                     /locus_tag="HUK62_RS17105"
                     /old_locus_tag="HUK62_17105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870947.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_087554524.1"
                     /translation="MANDLVVKNNALIDASYTLSLVEQRLIGLALVKANNQHQEITSDT
                     VLTIHAGEYAEQFKVDGSVAYRALKEASERLFLRYFSYTLYGLEFGKEYTFKRPKKLRD
                     CDIPTVMKSRWVQKIGYTESEGLLHFQLTSDVVLLVANSKEYFTSYYLSQTTDFTSTYA
                     TRLFELLMKWKNVGHIPFIEIEQLRGQLGVEPKQYKIISNFKLRVLDVAVEQVNQHSDY
                     TIKYEQHKQGRTIIGFSFKFKPKVDKISKKIISKQNRSNPDFFIKLSDPQRHLFANKMS
                     EMPEMSKYSQGTESFQQFAIRIADMLLEPEKFRELYPCLEKAGFQA"
                     /name="repM"
     gene            3586..3882
                     /locus_tag="HUK62_RS17110"
                     /old_locus_tag="HUK62_17110"
     CDS             3586..3882
                     /locus_tag="HUK62_RS17110"
                     /old_locus_tag="HUK62_17110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004779687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_176538533.1"
                     /translation="MMKLTNLLEEFHGTRAEYLDIVNYEIARENICSYIFLLSRISQNA
                     EPTEKMQMEGKIEDLIYYRDNLQIEDKENIQKVLAELIPEYKAEQEKQRAKKN"
                     /name="hypothetical protein"
     gene            complement(3879..4190)
                     /locus_tag="HUK62_RS17115"
                     /old_locus_tag="HUK62_17115"
     CDS             complement(3879..4190)
                     /locus_tag="HUK62_RS17115"
                     /old_locus_tag="HUK62_17115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004647573.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="WP_004870952.1"
                     /translation="MNKADLIAKLAPKLNINQDEARVVLNSVLEVITEALVEDKNVRLV
                     DFGTFEVKQRAERMGHNPKTGEKLKIEAKEIVTFKQGKALGEQFIVKPKQKSKAKKKA"
                     /name="HU family DNA-binding protein"
     gene            complement(4193..4480)
                     /locus_tag="HUK62_RS17120"
                     /old_locus_tag="HUK62_17120"
     CDS             complement(4193..4480)
                     /locus_tag="HUK62_RS17120"
                     /old_locus_tag="HUK62_17120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004647572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="WP_176538534.1"
                     /translation="MTKSAENIEKKIEAQLEKLKQLKAQKQAIEAREKSKQKEQERKDD
                     TRRKILLGSYLIKKMQSNEANKEKILTELNEYLTENRDRQLFDLPNIEEN"
                     /name="mobilization protein"
     gene            4690..5660
                     /gene="mobQ"
                     /locus_tag="HUK62_RS17125"
                     /old_locus_tag="HUK62_17125"
                     /pseudo=""
     CDS             4690..5660
                     /gene="mobQ"
                     /locus_tag="HUK62_RS17125"
                     /old_locus_tag="HUK62_17125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005265044.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /name="mobQ"
     gene            5657..6154
                     /locus_tag="HUK62_RS17130"
                     /old_locus_tag="HUK62_17130"
     CDS             5657..6154
                     /locus_tag="HUK62_RS17130"
                     /old_locus_tag="HUK62_17130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004969149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_176538535.1"
                     /translation="MSNQNDLDDQLYILLASMKEYREAIADDKKRLEQFYNQVASEVLD
                     KVEKSLENANKKHTSALNNSIQQLNQAISRLNSKFIIIFASTFVTLVMVFILAIFLYVP
                     SKDEIDERRADMAVLKKYPLQIRESDGETLVRIMTKKCYSFEPNNVKNKTYDWCRIDPK
                     KY"
                     /name="hypothetical protein"
     Typing          2607..3589
                     /name="rep_cluster_1071"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.904"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4691..5235
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.11"
                     /coverage="100.0"
                     /evalue="6.54e-104"
ORIGIN
        1 agcgatagtc atccaaaaat ttcggaatgg ctatccaact gatgcagttc taaaatatcg
       61 tcaccaacaa gcctataaat caagactaaa tcaggcttca catgacaatc acgacaattc
      121 ttaaactctg caccaccctt caagggatga tctaaatatt tttcaggcaa aggattattt
      181 gcgactaaac aatctaatac ttctgcccat tcagctgtaa ccagttctaa ataacgtctt
      241 ttaatatcac gtttaaaaga actggttacg ataatcttac gtttagccat gtgcttcttc
      301 cgacatagct ttaatcgctt cttctaccgt ttcgtactcg gtgtactcgc cattttcaat
      361 ctcttttaaa gattgaagta atttggctgc tgctttagga gttaaagcag tttctcgcgc
      421 atagtcaaaa gataaaggga tcgccttagt tttaacgatt tgattcaaga acagtttgaa
      481 tgcttggggc atagtcaaac cgtagtcttc caaaataggt gtgacttgtt cttttaactc
      541 tgcttctaag cgcatattga aattgactgc ggacatgatt tcctcactca aaaaccatta
      601 aagtaaagat aatataaggc atattcttta ctttttcatt acttttgatg taataaatgg
      661 tttctaacaa ctaaatttcg tataacgccc attatgttaa atggatgata aaaaatttat
      721 ctattgtaag aataggggat acttcatccc cctattctct taaatatctt agaagttgta
      781 acgaatacct aaagagtagt catgtgatat taagtccgct gtatttttaa aatttgcacc
      841 atcgaaagat aggctagtct caatttctcc agcatcgaca taacgataag ctaaatcaag
      901 agccacatct ttagttactt gataagctac acctgcacct gctgtccaag cgaagttatt
      961 atttgtatct gtagagatat gctcagaaga attaaacatt tcagggttag ttgaaatgtc
     1021 attttcaata cggcttacac ctaagccagc acttaaatat ggcgtaaatt tagttgtatt
     1081 tttaaaatcg taatagccat ttagcattaa agactgagtt ctaagttcat ttactacata
     1141 actaggaatg tttggtggtg cttgctcttc gttaccattg aaaatacctg agctgatatt
     1201 aggcgcgaat gtagctttat ctttatatgt atattctagt tcagcacgag catttacttt
     1261 acttacctta ctaaaatcaa aaccgatagc agctgagata tttgggtaaa tactttcatc
     1321 tgcatcttta tgtttaaaga tgatgctctc atcaccctga gccagtgtca atttatagtc
     1381 ttcaaacttt gtatcgctaa taccagcttt taatgatgta taaaaaccgc tatctgcata
     1441 agttaaactt gaaaatgaaa tggcagttac taatagaaga cgcttcataa gtgtttgttc
     1501 tctgtaatta ttatgaggat tgtacatctt ttttgttatt ttttgatact tctttagtat
     1561 taatatttaa gagtataggt ttcattttgc ttatcaaatt ttatcttttt gctacttttt
     1621 aaacaatttc ttttaaatcc agtaatataa ccaatttttt ttcgtaaaat aattgctaat
     1681 ttttgttaat ttatgtgtaa ttgctaacta tatttttact agttattaat catggatttc
     1741 tatcttatat gtatactctt tttacttata ttagctgttt ggttagtcta ttcgctatat
     1801 aaagaatgga ggaatgatcc tttcagtgat aagaatggtc cttctaagaa ataatcatta
     1861 aagtagtatc tataagaaca aaatattaag tttccctaaa attaacataa tacaccttat
     1921 acgaaatgtg ttataatttt ccttagaaat caatgctgta gtttctagtt gatagcccat
     1981 cttccccaag gtgggttttt tttatgattt ttcacgttcc acgcctattc tttgatcaat
     2041 gtttcacaac tttgattagc tccattaaat aattaaaacc tagataataa gaaccgagcg
     2101 agtgtctacg agcgattgag catcatcaaa atttcacgct cgccttttct ctgattacaa
     2161 gctccctttc gattttaaac ggtaggtaca gagcatcccg aatgggtgcg aactttggag
     2221 cttttgcttt tcaaaaagta tgagcgaagc gaatacatta ccaatgcttt tgcttttaga
     2281 tttcacaaac aggattaacc aaaaactgcc ccacgaagcg aggaacgagc ttcaatagag
     2341 tacgagcttt agcgagtacc tagggcagtt tttagcactc ccctcttttt aataatttta
     2401 taattttata attttagacc acttattcat attaataatc aatggcttat attgctatat
     2461 ctatacgttt atgcagtctt atctatacgt ttatgcagtc ttatctatac gtttatgcag
     2521 tcttatctat acgtttatgc agtattgcta tacgtattta atctatgtaa tataattaca
     2581 actaaaaaga atgtatgtat aagaaatggc aaatgattta gttgttaaaa ataatgcgct
     2641 gattgatgca agttatacct tgagcttagt agaacaacgc ttaattggat tggctttggt
     2701 caaagctaat aatcagcacc aagaaattac cagtgataca gttctgacca tacatgcagg
     2761 ggaatatgct gaacaattta aggttgatgg ttcagtagct tatcgagcat taaaagaagc
     2821 atcagaacgg ttgtttttac gctatttctc atacacctta tatggcttgg aatttggtaa
     2881 ggaatacact tttaaacgtc ccaaaaagct aagagattgc gatataccaa ccgttatgaa
     2941 atcacgatgg gtacaaaaga tcggttatac agaatcagag ggtttattac acttccaact
     3001 taccagtgat gttgtcctat tggttgctaa ttcaaaagaa tatttcacaa gctattactt
     3061 atcacaaact accgacttta ccagtaccta cgctactcga ttgtttgaac tcctaatgaa
     3121 atggaaaaat gtaggacata ttccatttat tgaaatagaa caattacgag ggcaattagg
     3181 tgtagaacct aagcaatata aaattatttc taactttaag ttgcgagtcc tagatgtggc
     3241 tgtggagcaa gtgaatcaac attcagacta tacgattaaa tacgagcaac ataagcaggg
     3301 gcggacaatt ataggttttt cattcaaatt taagcctaaa gtagataaaa tctcaaaaaa
     3361 aataatatct aagcaaaatc gtagtaaccc tgattttttt ataaaattga gcgatccaca
     3421 acgccattta tttgcaaata aaatgtctga aatgcccgaa atgagcaaat actcacaagg
     3481 tacggaaagc tttcagcaat ttgctattcg tatcgctgac atgcttttag agcctgaaaa
     3541 gttcagagag ctttatccat gtttagaaaa agcagggttt caagcatgat gaaattaacc
     3601 aacctattag aagaatttca cggcacacga gctgaatacc tcgatatcgt aaattatgaa
     3661 attgcgaggg aaaatatatg cagttatatc ttcctattat cccgaatatc ccaaaatgcc
     3721 gaacccaccg aaaaaatgca gatggaaggc aaaatagagg acttaattta ctatcgggat
     3781 aatttgcaga tcgaagataa ggaaaatatc caaaaggtct tagctgagct aattccagag
     3841 tataaagccg aacaagaaaa acaaagagct aagaaaaatt aggctttctt cttagcttta
     3901 cttttttgct ttggcttaac aataaattgc tctcccaaag ccttaccttg tttaaaagta
     3961 acgatttcct tagcttcaat tttaagtttt tcgccagttt taggattgtg ccccattctt
     4021 tcggcacgct gttttacttc aaaagtccca aaatcaacta atcggacatt tttatcttca
     4081 actagggctt ctgtaatgac ttcaagaaca gaattaagaa caactcttgc ttcgtcttga
     4141 ttaatattta atttaggtgc taatttggca attaaatcgg ctttattcat aattaatttt
     4201 cctcaatatt tggtaagtca aaaagctgtc tatctctatt ttcagttaaa tattcattaa
     4261 gttccgtgag tattttttct ttgttggctt cattactttg catcttttta attaaataag
     4321 aaccgagcaa aattttacgt cgagtatcat cttttctttc ctgctccttt tgctttgatt
     4381 tttctcttgc ttcgatagcc tgtttttgag cttttaactg tttcagcttc tctaactggg
     4441 cttcaatttt cttttcgata ttttcagcag attttgtcat aacggttaat ttcgggatat
     4501 agaaaaccac tcaagcataa ggccctatga attgaaattc aagcgggcta tatgctatcg
     4561 tgtttttcat gaaatgcgca cttacgactt ggatttcatc caagtcaaag tttgcgtaag
     4621 gggaatcccc ttaaccccaa aaaactgcta cgcaattttt taaaagcaaa agcatctcaa
     4681 ggcgactgca tggcaatcta tcattgttca actaaaacag ttaaccgaag ttcaggacga
     4741 actgcggttg catcaagtgc ctatcgtgca ggtgaaaaac tagaagatga acgcacaggg
     4801 ctaacccatg actttacgag aaaagacggt gtcgcccatt ctgaaattat ctctaattta
     4861 gatattgaaa ttgaccgtgg cgaactttgg aacttggcag aaaaaaccga gaaccgcaaa
     4921 gatgcccgaa ccgctagaga atgggtgatt gccctgcctg atgaattaga cgctgatcaa
     4981 cgtaaggact tggcaaaaga ctttgccagg tccttagttg atcgctatga cgtaatcgca
     5041 gatttagcca tccatgaccc gagtaaaggc ggtaatgata aaaaccatca cgcccatatc
     5101 atgctcacaa cccgaaaagc cgaattggac gcagacaata aactcactct gaacaccaaa
     5161 accgatattg aactcagcaa cgccaaacga aaaagccttg gtatgggaca acccaagaag
     5221 acattaagca aattagagaa acatgggcag agttagccaa taaagcattg gaacgtgcag
     5281 gctaccgaga aaaaatagat caccgcagct atgccgatca gaacaacgga ctacaagcca
     5341 ccatccatga aggcaccaaa gtcactcaat tacgcagaca aggcatagac actgaaatta
     5401 gccgtttcaa tgacaacgtc aaacagcaga atacccaaca acttgaccaa cagaaacagc
     5461 agaaagagag cgttttacag cgtggtttaa accgtgtcga tcaaggtttt gatcaatggc
     5521 agaagaacca agaaaccaaa cgtctagaac tggaacgcca agcggaaatg aagcgacagc
     5581 aagaattaga taaacaacga gccgagcaag cactccgcaa ggcatctcaa aaactaagta
     5641 gaggcggaat gtcattatga gtaatcaaaa tgatctagat gaccagttat atattttatt
     5701 ggcatccatg aaggaatatc gagaagccat agcagacgat aagaagcgat tagagcaatt
     5761 ttacaaccaa gtcgctagtg aagtactaga caaggttgaa aaatcgctag aaaacgcaaa
     5821 taaaaagcat acaagtgcat taaacaacag tattcaacag ttaaatcaag caatcagtag
     5881 acttaactca aaatttatca tcatttttgc cagtaccttt gtcacattgg tgatggtgtt
     5941 cattttggca atcttcttgt acgtgccaag taaagatgaa attgatgaac gtagagctga
     6001 tatggctgta cttaaaaaat atccgctaca aattagagaa agtgatggtg aaacgcttgt
     6061 cagaattatg acgaagaaat gctattcatt tgagccaaat aacgtcaaaa ataaaactta
     6121 tgactggtgt cgcatcgacc ctaaaaaata ctaggcgatc gatactttgc ttggtttttc
     6181 tcattactga tcagattaca gcaagcgcaa agttgcccga ctttgaaaca tggctaaagc
     6241 catctttcaa agcatcg
//
