LOCUS       NZ_CP055226             3125 bp    DNA     circular CON 01-FEB-2024
DEFINITION  Staphylococcus aureus LAC plasmid unnamed, complete sequence.
ACCESSION   NZ_CP055226
VERSION     NZ_CP055226.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15239172
            Assembly: GCF_015475575.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus LAC
  ORGANISM  Staphylococcus aureus LAC
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Jenjaroenpun,P. and Alkam,D.
  TITLE     Staphylococcus aureus strain LAC
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Jenjaroenpun,P. and Alkam,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2020) Biomedical Informatics, University of
            Arkansas for Medical Sciences, 4301 W Markham St, Little Rock, AR
            72205, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: FEB-2020
            Assembly Method                   :: Unicycler v. v0.4.4
            Assembly Name                     :: UAMS_Saur
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 1129.0x
            Sequencing Technology :: Illumina; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/01/2024 03:43:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,890
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,730
            CDSs (with protein)               :: 2,730
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 44 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 13 of 78
            Pseudo Genes (multiple problems)  :: 16 of 78
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP055226.1.
            The genome sequence presented here was generated using the USA300
            LAC strain used by Dr. Anthony R. Richardson at the University of
            Pittsburgh to generate a TnSeq library (Grosser et al., PLOS
            Pathogens, 2018). This library, and the parent strain, were
            obtained by the laboratory of Dr. Mark S. Smeltzer at the
            University of Arkansas for Medical Sciences (UAMS). The genome of
            the parent strain was subsequently sequenced, assembled and
            polished at UAMS by Dr. Piroon Jenjaroenpun and Ms. Duah Alkam.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Staphylococcus aureus LAC"
                     /mol_type="genomic DNA"
                     /strain="LAC"
                     /host="Homo sapiens"
                     /db_xref="taxon:867914"
                     /plasmid="unnamed"
     gene            complement(150..1145)
                     /locus_tag="HUW68_RS14410"
                     /old_locus_tag="HUW68_14415"
     CDS             complement(150..1145)
                     /locus_tag="HUW68_RS14410"
                     /old_locus_tag="HUW68_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001199892.1"
                     /translation="MQYNTTRSITENQDNKTLKDMTKSGKQRPWREKKIDNVSYADILE
                     ILKIKKAFNVKQCGNILEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQKVI
                     EKVIKEKPKARWLFLTLSTKNAIDGDTLEQSLKHLTKAFDRLSRYKKVKQNLVGFMRST
                     EVTVNKNDGSYNQHMHVLLCVENAYFRKKENYITQEEWVNLWQRALQVDYRPVANVKAI
                     KPNRKGDKDIESAIKETSKYSVKSSDFLTDDDEKNQEIVSDLEKGLYRKRMLSYGGLLK
                     QKHKILNLDDVEDGNLINASDEDKTTDEEEKAHSITAIWNFEKQNYYLRH"
                     /name="protein rep"
     gene            1597..2178
                     /locus_tag="HUW68_RS14415"
                     /old_locus_tag="HUW68_14420"
     CDS             1597..2178
                     /locus_tag="HUW68_RS14415"
                     /old_locus_tag="HUW68_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000991705.1"
                     /translation="MNAIQNSISLRKILVVSLSILTILSMVLDFNFKEAQAQNKNNIAD
                     KNVETLNEKEIEKELKYIYGKIIILDKDGTAKDVNLENAKSRYGYVPEGFQKLKNDIEN
                     KKKTSSISPRAVGGNYKNSGDCFYSEVLNSYGELLTGNIITAVFEDVKAKNLKGVAKKL
                     AKIGIKGNLAGIAATMVSKDVQCNLKYGLL"
                     /name="hypothetical protein"
     gene            complement(2504..2692)
                     /locus_tag="HUW68_RS14420"
                     /old_locus_tag="HUW68_14425"
     CDS             complement(2504..2692)
                     /locus_tag="HUW68_RS14420"
                     /old_locus_tag="HUW68_14425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001039248.1"
                     /translation="MNKNTVLDEGYVIATILNVFFLLGLIFISHLENLYILIPYVILMG
                     INAIYLVVKVMNFKKNN"
                     /name="hypothetical protein"
     Typing          complement(2..80)
                     /name="MOBV"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.55e-33"
     Typing          complement(3041..3125)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="7.17e-37"
     Typing          complement(151..1145)
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.9"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aagtgatatc atacaaagct gtactgtctt atttttgtga cacaatatat tgcgtttgtc
       61 ttatttttgt gacactacat acagcatttg tcacaaaaat aagacagtac ttttttataa
      121 aaaatcaaca aaaaagacat tagtcaacac taatgtctta aataataatt ttgcttttcg
      181 aaattccaaa ttgcggtaat tgaatgtgct ttttcttctt cgtctgttgt tttatcttca
      241 tcacttgcat taatcaaatt accatcttcg acatcgtcta agtttaaaat tttatgtttt
      301 tgtttaagca atccaccata acttaacata cgttttcgat acaaaccttt ttctaaatca
      361 cttacaattt cttgattttt ttcatcatca tcagttaaaa aatcagatga tttaaccgaa
      421 tattttgagg tctctttgat tgccgattca atgtctttat cgccttttct attcggtttg
      481 atcgctttaa cattagcaac aggtcgatag tcaacttgta atgctctttg ccataaatta
      541 acccattctt cttgagttat ataattctct ttttttctaa aatatgcatt ttcaacacat
      601 aacaaaacat gcatgtgctg attataacta ccgtcatttt tattaacggt aacttctgtt
      661 gaacgcataa atccaacaag attttgttta acctttttat atctactcaa cctatcaaat
      721 gctttagtta gatgcttcaa actttgttct aaagtatctc catctatcgc attttttgtt
      781 gaaagtgtta aaaacaacca acgtgctttt ggcttttcct taattacttt ttcaatcact
      841 ttttgagctt gataactatt tttcatagca cgtctccaat tacaaaccgg acataatttt
      901 gatttacaaa accatgtctt atgtaacttc aaatagcctt catcagttgg cttaaattct
      961 aaaatattac cacattgttt tacattaaaa gcctttttga tttttaaaat ttctagtata
     1021 tctgcatagc ttacattatc tattttcttt tctctccatg ggcgttgttt cccacttttc
     1081 gtcatatctt ttaacgtttt attatcttga ttttcggtta tacttctagt agtattatat
     1141 tgcatataaa aagaccccca gtattattta agattcgaca ccttaataat ataactgggg
     1201 ttttttattg tcaaattttc ctttcacgca caaaaacacg tttaaacact gtaatatcaa
     1261 cagttacagc tatatatata aaatcgagtt atttctatat agtatcaaga caagaagaaa
     1321 ctcgttcaca ctcgtttcaa aaaacaacct attccttctt ctttttatga ccctctaaac
     1381 ctaaataaat taatgtgaaa accaatgaat aaataaaact ttctaaaaag tcaaaaggat
     1441 tatcaaataa aatcgaatta acaaaattaa aaattaaaaa tacagataat aaaataatca
     1501 aaaagtaaaa aaacttattc atattaaact cctttgtttt tatatgttaa tattatattg
     1561 aattcattta aataacaaaa caagggggca tttacaatga atgcaattca aaacagtata
     1621 tcactaagaa agattttagt agtatcttta agtattttaa ccatactttc aatggtcttg
     1681 gattttaatt tcaaagaagc tcaagcccaa aacaaaaata acattgcaga taaaaacgtt
     1741 gaaactttaa acgaaaaaga aattgaaaaa gaactaaaat acatatatgg caaaattatt
     1801 attttagata aagacggaac agctaaagat gttaatctag aaaatgccaa atctcgatat
     1861 ggatatgttc ctgaaggatt ccaaaaacta aaaaacgaca tagagaataa aaagaaaact
     1921 agttcaatat cacctagagc agttggtggg aactacaaaa atagcggtga ctgtttctat
     1981 tctgaagttt taaactcata tggagaactt ttaactggaa acataatcac agcagttttt
     2041 gaagatgtta aagccaaaaa cttaaagggc gtagcaaaaa aattagctaa aattgggata
     2101 aaaggaaacc tagctggaat cgcagctact atggtttcga aggatgtaca atgtaatctt
     2161 aaatacgggt tactttagat actatttatt ccaattgctt tattgacgtt gagcctcgga
     2221 acccttaaca atcccaaaac ttgtcgaatg gtcggcttaa tagctcacgc tatgccgaca
     2281 ttcgtctgca agtttagtta agggttcttc tcaacatcaa taaattttct cggcataaat
     2341 gcgtcgtcta ttttttatta tcttcatgat aatcatattt ttcttccatc tcgtcaaaag
     2401 catcatcttt atagaaaaac aaaaagtaag caacaacacc aataggaata aaaaaacagt
     2461 gtgaaaacaa gaagaaactc gtttcaaaat cccctcaaat attctaatta ttctttttga
     2521 aattcataac cttaacaacc aaataaatag catttattcc catcaaaata acatacggaa
     2581 ttaatatata taaattttcc aaatgagata taaaaattaa tccaagtaaa aagaaaacat
     2641 ttaaaatagt tgctataacg taaccttcat ctaacactgt gtttttattc aaaataaaca
     2701 ctcctttttt aaaaaacttt atcacaagac ttactcaaat tataaatacc tttattttga
     2761 gttttaaggg ggtattttaa aggtttaggg ggtatcatat aggtttttaa cttaaaaata
     2821 tatacaagct cttaaaacgc aaatatgagc caaataaata tatggtaatt tcacaaacaa
     2881 aaatttcagc aaaaccagtg actaattttt taacactgcc catttacatg caaattaaaa
     2941 attagcataa aatcctttaa aacttccaca tgtttttcta aattcctgca gggcgttttt
     3001 tatcaaaaat cgagaatttt taatttttct ctaaaaactc cgtaagagca taaggagaat
     3061 ttgctatcat tcaaaaatga taggtctggc aaagccagaa attccagggg ttttaaggta
     3121 tttta
//
