LOCUS       NZ_CP055191             4167 bp    DNA     circular CON 01-MAY-2024
DEFINITION  Klebsiella pneumoniae strain STEFF_14 plasmid unnamed5, complete
            sequence.
ACCESSION   NZ_CP055191
VERSION     NZ_CP055191.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13925530
            Assembly: GCF_022354285.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Che,Y., Xu,X., Yang,Y., Brinda,K., Hanage,W., Yang,C. and Zhang,T.
  TITLE     High-resolution genomic surveillance elucidates a multilayered
            hierarchical transfer of resistance between WWTP- and
            human/animal-associated bacteria
  JOURNAL   Microbiome 10 (1), 16 (2022)
   PUBMED   35078531
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   Che,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JUN-2020) Civil Engineering, The University of Hong
            Kong, Pokfulam Road, Hong Kong 000000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: "Other" v. Unicycler
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology :: Illumina; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022354285.1-RS_2024_05_01
            Annotation Date                   :: 05/01/2024 05:13:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,397
            CDSs (total)                      :: 5,273
            Genes (coding)                    :: 5,140
            CDSs (with protein)               :: 5,140
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 58 of 133
            Pseudo Genes (incomplete)         :: 78 of 133
            Pseudo Genes (internal stop)      :: 20 of 133
            Pseudo Genes (multiple problems)  :: 20 of 133
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP055191.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="STEFF_14"
                     /isolation_source="Shatin WWTPs_14 WWTPs effluent"
                     /db_xref="taxon:573"
                     /plasmid="unnamed5"
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.4 N 114.2 E"
                     /collection_date="2018-12"
     gene            complement(join(3521..4167,1..469))
                     /locus_tag="HUZ42_RS26890"
                     /old_locus_tag="HUZ42_26850"
     CDS             complement(join(3521..4167,1..469))
                     /locus_tag="HUZ42_RS26890"
                     /old_locus_tag="HUZ42_26850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405083.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_012579087.1"
                     /translation="MIKEISLKNFKCFNELYLKQLKTLNIIAGKNNYGKTSILDAIFCF
                     YDVKNPAVLLNIQAFRKEMAEINKNKPFWVSYFHDMDTSQKMSIVIRDERSEVTQTYET
                     ETNQRLESSLSLNVDTVLSNQNIPRQTIGSQATVNSLKINVTETPNAKNRVSNKLFSMQ
                     LKEDGVEIKSEIKNERDGINYKFKTATIITTSRKINKEATITNVSSLLTQKRKKDILEN
                     LKKIDDRIVDIAISAIGNNKEIYLDIGFSELNEISMLGEGISRALSFISSVLVQENSII
                     LIDEIENGIHYSVIKDIIKSLISSAKQNNNQIFATTHSQDVIRAINEIDSKNEDIAYIR
                     LGREKNSLKPTAVQFNMDDFSYSVENDWEVR"
                     /name="ATP-binding protein"
     gene            complement(814..927)
                     /locus_tag="HUZ42_RS26895"
                     /pseudo=""
     CDS             complement(814..927)
                     /locus_tag="HUZ42_RS26895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            1343..1534
                     /locus_tag="HUZ42_RS26900"
                     /old_locus_tag="HUZ42_26855"
     CDS             1343..1534
                     /locus_tag="HUZ42_RS26900"
                     /old_locus_tag="HUZ42_26855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1534..1935
                     /locus_tag="HUZ42_RS26905"
                     /old_locus_tag="HUZ42_26860"
     CDS             1534..1935
                     /locus_tag="HUZ42_RS26905"
                     /old_locus_tag="HUZ42_26860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_012579088.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2281..2523)
                     /locus_tag="HUZ42_RS26910"
                     /old_locus_tag="HUZ42_26865"
     CDS             complement(2281..2523)
                     /locus_tag="HUZ42_RS26910"
                     /old_locus_tag="HUZ42_26865"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_098963735.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     gene            complement(2889..3521)
                     /locus_tag="HUZ42_RS26915"
                     /old_locus_tag="HUZ42_26870"
     CDS             complement(2889..3521)
                     /locus_tag="HUZ42_RS26915"
                     /old_locus_tag="HUZ42_26870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3226 domain-containing protein"
                     /protein_id="WP_012579086.1"
                     /translation="MKDINNSKKIIFVEGDDDFHFLCNLLEIQGITDVFVEKINGKSKL
                     KQALRAFKNIDSFDEKESIFIILDADTSFSDTERSIIATLRELQISSPSSHNEIAMNGN
                     TKISFFIMPGKDKQGAIEDLIIEHASTRALYEYIIEFFEKIKNNKDDISASDKEYTYPT
                     NEKKAKVQVYLSCNRESDSRIGISMKNGIINTADNCFDEIKDFISKI"
                     /name="DUF3226 domain-containing protein"
     Typing          complement(815..1222)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.399"
                     /coverage="99.0"
                     /evalue="5.92e-171"
ORIGIN
        1 tggaaaccct gtttttcgca ttgggtgtct cggtcacatt tatttttaaa gagtttactg
       61 tggcctgaga accgatagtt tgacgaggta tgttttggtt agatagtacg gtgtcaacgt
      121 ttagagacaa acttgattct aatctctgat ttgtttctgt ttcatatgtt tgtgttactt
      181 cagagcgttc atctcgaatg actatggaca tcttttgtga tgtgtccata tcatgaaagt
      241 aactaaccca aaatggcttg tttttgttta tttctgccat ttcttttctg aatgcttgaa
      301 tgtttaacaa aacagccggg ttttttacat cataaaaaca aaaaatagca tccagtatag
      361 atgttttccc gtagttgttt ttcccagcta tgatatttaa agtctttaat tgctttagat
      421 atagctcatt gaagcatttg aagttcttta aacttatttc ctttatcatg atagcctctg
      481 ttcgtgtttt tgttatatgc catacgattt gttagtacct taataaaatg tacagctttc
      541 attgtgatta gctagacttt atgtcaaaga ttaactttga ttgcttgtgt tttaatcgat
      601 tttttttact ttcttcttga gattatctgg cagcagccgt aagcttccgg ctgtgaacga
      661 aaaaaaccgc cttggcgggc ggttttttcg aaaggttcag tagagttggc gctctatgaa
      721 ccgaggtaac tggcttaaag ggagcgcagt caccaaaact gttcgtttag tttagcttta
      781 actggcgtat aacttcaaga ctaactccct aaatcagtta ccagtggctg ctgccagtgg
      841 cgctttgtcg tgtctttccg ggttggactc aagacgatag ttaccggatt aggcgcagca
      901 gtcggactga acggggggtt cgtgcataca gtccagcttg gagcgaactg cctacccgga
      961 actgagtgtc aggcgtggaa tgagacaaac gcggccataa cagcggaatg acaccggtaa
     1021 accgaaaggc aggaacagga gagcgcacga gggagccatc agggggaaac gcctggtatc
     1081 tttatagtcc tgtcgggttt cgccaccact gatttgagcg tcagattccg tgatgctcgt
     1141 caggggggcg gagcctatgg aaaaacggcc gctgtgcggc ctttccttct ttcctccatg
     1201 cgattactcg gttttctcga gcgtttctac gcatgctcct gccgctcgcc gcagccgaac
     1261 ggcaggagcg tagcgactga gtgagcgagg aagcggaaaa gaggagatcc ctacactgac
     1321 cactgacgct ctgaggcctc ttatgacgaa acaacagaaa acagcgctga acatggctaa
     1381 attcatccag aatcagtctc tgctcctgct ggagaagctt aacgagcttg atctcgatgt
     1441 cgaggccgac ctttgtgaga aactccacga tgatgcggag catcttttcc gtacactgtc
     1501 ctcgagactg gatgcattac aggatggtaa ctgatggcca gagtgtctat tagtgaggct
     1561 gcccgactga cgggtaagag tagaacaaca ctgcacaggt taattaaagc aggtgacctg
     1621 tccacctgta gcggtgagcg caatgcaaaa atggtagata cttcagagct tttacgtgtc
     1681 tttggcccct ttgagcaacc gaagggtgaa caccattctg gacaggtctc tggacagcgt
     1741 gatacagcct ctgctgacca gagtgaacag gtgatccgtc atctgaaaca ggagattgaa
     1801 cacctgaaca ccttactttt gtccaaggat tcgcacattg acagcctgaa acaggccatg
     1861 ctccttcttg aaaatcacca ggcaaaatta actgaatccc cggcgccatg gtggaaattc
     1921 tggaaaaaat cctgaccccg tcatgcttca gttttcctct ccggccatcc cagggagttc
     1981 ctgaaccttc ccaaaaatcg tcagatctct gaacgctcct caccactaaa aatacccaca
     2041 ccccacgcaa aattaaaatt ttgcagattc cttatttaca acaatgggtt aatgaaaaat
     2101 aaatgtgcgg tgatgtgtta caaagtgcag atccaagtcg tggacgactc tatggcgaat
     2161 cgctgacgaa ttgcccacga ttcatatgtg aatcacgctt tattatcata ttgttatgat
     2221 gaatcgactc ttgattcaat tcatcacttt gggtgttttt tgctcatggt gactcttgat
     2281 tcaattcacg attcgaatcg ccggtgaatc gtgtggtgag tcgtccggcg acttgccata
     2341 acgtgattgc cggccgtcga cgaacccgat tcctgaaggt gctgaaaacc ggttttccgg
     2401 ctgcgccggc aacgcttcac ggccacgcaa taaattgcgt ccccgttcac cgttttcgga
     2461 ggagaagaac gccggaaaca ggacttcccc tggctgaatg gtgtgaattt ccgcgctatg
     2521 cacttgcgcg catactcatg catgccgtaa aaacagagcc tgcgcgtttc tggcgggttt
     2581 tcgggtggtt tgttgcctgt tttaccggtt tcccgtcaga aacgtcctga gtccgtttta
     2641 gcggtgcgta caattatgat tttatgttaa attgttttaa gtgaatagag agaatataac
     2701 ttaagggagc atcttgtgct gggtctttta cattagatct gcacaatcat tgttttggca
     2761 tctggtgatg tctttaggac gtttgagatt gctgataact aaagtgtatg atgctttcta
     2821 tacgttatcc ggcgtttaat aacaccctgg tgataagcca gcccgtgaag ggctggcttg
     2881 atttggattt atatttttga aataaaatct tttatttcat caaagcaatt gtctgctgtg
     2941 tttattattc catttttcat ggatattcct attcttgagt cggattctct attgcagcta
     3001 agatagactt gtaccttagc ttttttttcg tttgttggat aagtgtactc tttgtcactc
     3061 gctgagatgt catctttatt atttttaatt ttttcgaaaa attctattat gtattcatat
     3121 aacgctcgag tgcttgcgtg ttcaattatc agatcttcaa tggcaccttg cttgtctttt
     3181 cctggcatta taaagaaaga aatttttgta ttgccattca tagctatctc gttatgcgag
     3241 ctgggggagg agatttgtag ttctcgcaat gtggcaatta ttgatctttc tgtgtcagaa
     3301 aatgatgtgt cagcatctaa tataataaaa atactttctt tttcatcgaa agaatcaatg
     3361 tttttgaatg ctctaagtgc ttgttttaat tttgattttc catttatttt ttcgacaaaa
     3421 acatcggtaa tgccttgtat ttcaagtagg ttgcatagaa aatgaaaatc atcatcaccc
     3481 tcaacgaaaa tgatcttttt gctattattt atgtctttca ttatcttacc tcccagtcgt
     3541 tctcaactga gtaggagaaa tcatccatat tgaattgtac ggctgttggt tttagggagt
     3601 tcttttctct tcctagtcta atataagcga tatcttcatt tttgctatct atttcgttta
     3661 ttgctcgtat cacatcttga ctatgagttg ttgcaaatat ttgattgttg ttttgttttg
     3721 cagatgaaat taatgatttt attatgtctt taattactga gtaatgtatt ccgttctcga
     3781 tttcatcaat aagaataatt gagttttcct gtaccagcac tgagctgata aaagataatg
     3841 ctcgactgat accctcgcca agcattgata tttcatttag ctcactaaat ccaatgtcta
     3901 aatatatttc cttattgtta cctatcgcag atattgctat atctacaatt ctgtcatcta
     3961 ttttttttaa gttttctagt atgtcttttt tccttttttg tgttagtagg ctagatacat
     4021 ttgtaattgt tgcctctttg tttatctttc tggatgttgt tattattgtt gctgtcttaa
     4081 atttataatt aatcccatcc ctttcgtttt ttatttcgga ttttatttct acgccatctt
     4141 ctttcaattg catagagaaa agcttat
//
