LOCUS       NZ_CP055169             6817 bp    DNA     circular CON 03-MAY-2024
DEFINITION  Shigella flexneri strain STEFF_24 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP055169
VERSION     NZ_CP055169.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13925538
            Assembly: GCF_022353545.1
KEYWORDS    RefSeq.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6817)
  AUTHORS   Che,Y., Xu,X., Yang,Y., Brinda,K., Hanage,W., Yang,C. and Zhang,T.
  TITLE     High-resolution genomic surveillance elucidates a multilayered
            hierarchical transfer of resistance between WWTP- and
            human/animal-associated bacteria
  JOURNAL   Microbiome 10 (1), 16 (2022)
   PUBMED   35078531
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6817)
  AUTHORS   Che,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JUN-2020) Civil Engineering, The University of Hong
            Kong, Pokfulam Road, Hong Kong 000000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: "Other" v. Unicycler
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology :: Illumina; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022353545.1-RS_2024_05_03
            Annotation Date                   :: 05/03/2024 04:57:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,882
            CDSs (total)                      :: 4,762
            Genes (coding)                    :: 4,562
            CDSs (with protein)               :: 4,562
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 200
            CDSs (without protein)            :: 200
            Pseudo Genes (ambiguous residues) :: 0 of 200
            Pseudo Genes (frameshifted)       :: 80 of 200
            Pseudo Genes (incomplete)         :: 125 of 200
            Pseudo Genes (internal stop)      :: 41 of 200
            Pseudo Genes (multiple problems)  :: 43 of 200
            Pseudo Genes (short protein)      :: 2 of 200
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP055169.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6817
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /strain="STEFF_24"
                     /isolation_source="Shatin WWTPs_24 WWTPs effluent"
                     /db_xref="taxon:623"
                     /plasmid="unnamed2"
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.4 N 114.2 E"
                     /collection_date="2018-12"
     gene            join(6712..6817,1..8)
                     /locus_tag="HUZ50_RS24285"
     CDS             join(6712..6817,1..8)
                     /locus_tag="HUZ50_RS24285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071821494.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAATGN"
                     /name="replication initiation protein"
     gene            complement(48..233)
                     /locus_tag="HUZ50_RS24290"
                     /old_locus_tag="HUZ50_24085"
     CDS             complement(48..233)
                     /locus_tag="HUZ50_RS24290"
                     /old_locus_tag="HUZ50_24085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005421809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_094307661.1"
                     /translation="MSKKDAKILLRSFFSARNLLLANKKTTATNGGLFAGSRATNSFSE
                     GNWLGGAQSPKPVFSV"
                     /name="hypothetical protein"
     gene            301..930
                     /locus_tag="HUZ50_RS24295"
                     /old_locus_tag="HUZ50_24090"
     CDS             301..930
                     /locus_tag="HUZ50_RS24295"
                     /old_locus_tag="HUZ50_24090"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_140430500.1"
                     /translation="MRHIFFLLYFFCISTIASNELKINNMPIELIFNDGSSAKISSCTD
                     LQNQMSLGKKIESIPNISDPDYNDIKNKIFICRMQNYISNHRMTRIKDSLSIHDVLSNF
                     PATAAYFISQEEMDKVSREYSNKTILEYTPDLIIDKNKAISQKKYTSYSLPEYYHFKNS
                     DGDEIKIVILAGHSIGGTASSRSYWRIDDDHSHPWTVTELDENSFL"
                     /name="hypothetical protein"
     gene            complement(994..1755)
                     /locus_tag="HUZ50_RS24300"
                     /old_locus_tag="HUZ50_24095"
     CDS             complement(994..1755)
                     /locus_tag="HUZ50_RS24300"
                     /old_locus_tag="HUZ50_24095"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_224728059.1"
                     /translation="MPGFNYGGYGDGTGWSSERGDGPAPGGGSHGNSGRDDHRGESGYG
                     YPGHNGISGPVSLRTTDSYDGDTMSGYINNLINHEGFKNTMYRDTEGNITVGIGHLLSS
                     PEMAAGLPFNRTRITHGHGDEMEHEFSVSREDITSSFNALRDNPTAISGIHISNDAVIG
                     LAISDVESTISGLKGLYSDFDSFPRSAKTALVDMGFNVGVGKLRSDFPNFNNAVNKKDW
                     NTAADESHRTKIGEGRNNDTKAQFQQAANGN"
                     /name="hypothetical protein"
     gene            2014..2451
                     /locus_tag="HUZ50_RS24305"
                     /old_locus_tag="HUZ50_24100"
     CDS             2014..2451
                     /locus_tag="HUZ50_RS24305"
                     /old_locus_tag="HUZ50_24100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="WP_170936219.1"
                     /translation="MGHCHFMAWHTLQDALKMTGRSRSQFYRDMRAGLVSYRTSQDGRR
                     EFETSELIRAYGELKRNETPERHTAGQAEKPHNQQTERILREVNELKQCLTLMLEDKQA
                     QDTERRRQEAEREQLQNEIVQLRQALEQEKKRGFWSRLFGR"
                     /name="entry exclusion protein 1"
     gene            2532..2960
                     /locus_tag="HUZ50_RS24310"
                     /old_locus_tag="HUZ50_24105"
     CDS             2532..2960
                     /locus_tag="HUZ50_RS24310"
                     /old_locus_tag="HUZ50_24105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_094307660.1"
                     /translation="MKRSGGTRYMYLSALITVTAALTACTPKTSLEQHTRHYVYASDDG
                     FDPNFYTKKADTTRMMIPFFQQFREMGVKDKAAGVSAEKAQQRIKEFHSEEFLQSLRTT
                     TNFAGRKYTNSDTPSPEKMKLLADTISAIYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            3185..3394
                     /locus_tag="HUZ50_RS24315"
                     /old_locus_tag="HUZ50_24110"
     CDS             3185..3394
                     /locus_tag="HUZ50_RS24315"
                     /old_locus_tag="HUZ50_24110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgH/BhsA/McbA-like domain containing protein"
                     /protein_id="WP_009310047.1"
                     /translation="MKSIKTFVAVAALSMMSFGAFAQSVSATASTLDRAEAKIAAQAAE
                     QGASYKITSAQFNNRVHMTAELTK"
                     /name="YdgH/BhsA/McbA-like domain containing protein"
     gene            complement(4017..4250)
                     /locus_tag="HUZ50_RS24320"
                     /old_locus_tag="HUZ50_24115"
     CDS             complement(4017..4250)
                     /locus_tag="HUZ50_RS24320"
                     /old_locus_tag="HUZ50_24115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021534848.1"
                     /translation="MTELEKHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMKLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4257..4787)
                     /locus_tag="HUZ50_RS24325"
                     /old_locus_tag="HUZ50_24120"
     CDS             complement(4257..4787)
                     /locus_tag="HUZ50_RS24325"
                     /old_locus_tag="HUZ50_24120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001314441.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSNE
                     SLIRNAINDHTTALKELLERYQKTTVSTMDTHWQTVLKMSVKRWLWLIIVSVLMFATTG
                     SLLWYQGMKINVNMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETNWTE
                     DNGKLNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            complement(<4825..5523)
                     /locus_tag="HUZ50_RS24515"
                     /old_locus_tag="HUZ50_24125"
                     /pseudo=""
     CDS             complement(<4825..5523)
                     /locus_tag="HUZ50_RS24515"
                     /old_locus_tag="HUZ50_24125"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(5465..5788)
                     /locus_tag="HUZ50_RS24335"
                     /old_locus_tag="HUZ50_24130"
     CDS             complement(5465..5788)
                     /locus_tag="HUZ50_RS24335"
                     /old_locus_tag="HUZ50_24130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000956000.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGTR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5853..6044
                     /locus_tag="HUZ50_RS24340"
                     /old_locus_tag="HUZ50_24135"
     CDS             5853..6044
                     /locus_tag="HUZ50_RS24340"
                     /old_locus_tag="HUZ50_24135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165974.1"
                     /translation="MTKQEKTALNMARFIRGQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(6130..6211)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.386"
                     /coverage="100.0"
                     /evalue="1.35e-30"
     Typing          6228..6784
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.238"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3914..5475)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.777"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtaactgatt tagaggagtt ggtcttgaag ttatgaacca gttaaggcta aactgaaaag
       61 acaggttttg gtgactgcgc tcctccaagc cagttacctt cggaaaaaga gttggtagct
      121 cttgatccgg caaacaaacc accgttggta gcggtggttt ttttgtttgc aagcagcaga
      181 ttacgcgcag aaaaaaagga tctcaagagg atcttggcat cttttttact caaaatactt
      241 tcctgtttaa atacattcag tacaaaataa attaacaact aatgtaggag aaccctaagt
      301 atgcgtcaca tattcttttt gctttatttt ttctgcatat caacaatagc ttcaaatgaa
      361 ttaaaaatta acaacatgcc tatagagtta atttttaatg acgggagcag tgcaaaaata
      421 tcatcttgca ctgaccttca gaatcagatg tcactaggaa aaaaaataga gtcaatacct
      481 aacatttcag acccagatta caatgatata aaaaataaaa tatttatatg caggatgcaa
      541 aattacatat caaatcatcg aatgacgaga atcaaagact cattatcaat acatgacgtt
      601 ttgagtaatt ttccagcaac agcggcatat tttataagtc aagaagaaat ggataaagtt
      661 tctcgtgaat acagcaacaa gacaatttta gaatacacac cggatcttat tattgataaa
      721 aataaagcaa tatcacaaaa aaaatacaca agctactcac tgcctgaata ttatcacttt
      781 aaaaatagtg atggtgatga aataaaaatt gttattttag cagggcattc aattggtggt
      841 acagctagct cacgctcata ctggcgtata gatgatgatc attcacaccc ctggacagtc
      901 actgaacttg atgaaaatag tttcttataa ttcaattaat taccacctca taaagaggtg
      961 gtataggcat gaaatatgta gaatccttta tgcttagtta ccattagctg cttgttggaa
     1021 ctgagctttc gtatcattat ttcggccttc acctatctta gttcgatgag actcatcagc
     1081 cgcagtattc caatctttct tatttacagc attattaaag tttggaaagt ccgatcgtaa
     1141 tttaccaact cccacattga accccatatc aactaatgcg gtttttgctg aacgagggaa
     1201 actatcaaaa tcagaataaa gcccttttaa tcctgaaatt gtcgattcta catcgctaat
     1261 agcaagacca attactgcat cgtttgaaat gtgaatacca gaaatagctg taggattatc
     1321 tctcaatgca ttaaatgaag atgtaatatc ttcccgcgat acagaaaact catgttccat
     1381 ttcatcacca tgtccatgtg taattctagt tcggttaaac ggtaatccag ccgccatctc
     1441 cggtgatgaa agcaagtgac caataccgac agttatgttc ccttcggtat ccctatacat
     1501 tgtattttta aaaccttcat gattgattaa gttgttaata taacctgaca tagtatcacc
     1561 atcataacta tctgttgtcc tgagactcac aggcccagat atcccattgt gaccaggata
     1621 accataacca ctttcaccac gatgatcgtc tcgaccacta ttcccatgag aaccgcctcc
     1681 tggagccgga ccatcaccac gttcagaact ccagccagta ccgtcaccat atccaccata
     1741 attaaatcca ggcataacta actcctcttt gacttgtaaa acaaaccgtt aaaaacaaat
     1801 actgtatata taactactgg ttttatatac agcttaaaaa ttacgccgct gcgttttccc
     1861 tgtcaaccct gtggattttc gtttttgtga aaacgatcaa aaaacagcca accacaccac
     1921 gtgctattcc cgcccgatgc cacaaaaacc agcacaaaca ttaccgttct ccggcctcag
     1981 tatgttttac tgaaaccatg atatgagaca ccaatgggac actgtcactt catggcatgg
     2041 cacacactcc aggacgcact gaaaatgacg ggcagatcgc gttcacagtt ttaccgtgat
     2101 atgcgcgcag gccttgtcag ttaccgtacg agccaggacg gacgacggga gtttgaaacc
     2161 agtgaactga tccgggcata cggcgaatta aagcggaatg agacaccaga aaggcacact
     2221 gcgggacagg cagaaaaacc tcataatcag cagacagaac gcattctccg ggaggtgaac
     2281 gagctgaaac aatgcctgac gctgatgctt gaggataaac aagcccagga tacggagcgc
     2341 cggcgccagg aagcagaacg ggaacaacta cagaatgaga tagtccagct caggcaggcg
     2401 ctggagcagg aaaagaaacg gggattctgg tctaggttgt tcggtcgctg aacgcgctca
     2461 gagactgatg ataagatagc catcagataa taactcaccg aaaataaata ctttaaggta
     2521 aggagactcg catgaaacgt tctggcggca cacgctatat gtatcttagc gccctgataa
     2581 ctgtgacagc agccctcaca gcctgtaccc caaaaaccag cctggaacaa catacccggc
     2641 attatgttta tgcttcagat gatgggtttg atcctaactt ctacaccaag aaagctgaca
     2701 ccacacggat gatgattccg ttctttcagc agttccggga gatgggggtg aaagacaaag
     2761 cagccggagt atcagcagaa aaggcacagc aacgcataaa agaattccac tctgaagaat
     2821 tccttcagtc actgagaacc acaaccaact ttgcgggcag aaaatacacg aacagtgata
     2881 cgccatcacc ggaaaaaatg aagctgctgg ctgacacgat ttctgcgatt tatctcgatg
     2941 gctacgaggg aagacagtaa gtggatttac cataatccct taattgaacg aaccctcaag
     3001 gctattcatc ctgcccctaa atcagagtct cacacctggt tttgattcgc tcaattttta
     3061 ttctattttt ttgaatgata tccgcaaatt gtttcgattt taatcagctc atggtcgtcc
     3121 tatcatttat cacatcaggg caacaacgcc cgatacagaa caaaattact taaggaatac
     3181 gaccatgaaa tccatcaaaa ctttcgttgc agttgctgct ctatccatga tgtctttcgg
     3241 tgcctttgct cagagcgtca gcgctaccgc ctccaccctg gatcgtgctg aagcaaaaat
     3301 tgccgcacag gctgctgaac agggtgcgtc atacaaaatc accagcgctc agttcaataa
     3361 ccgtgtccac atgacggctg aactgaccaa ataagatggt cgcagatgga gctgccgaac
     3421 aggtcgccgg gaggcggccc gtttcgccac taaacaggaa aaccctcaag ccccttctgg
     3481 ggcctttttg cgtctgttat ttaacataaa ggcatgattg tacgcaccgc tgaaacgcgt
     3541 tcggtgcgat cacggcagca gacaggtaaa aatggcaaca aaccacccga aaaactgccg
     3601 cgatcgcccc cgataaattt taaccacatg catagctatg cagccatgtg aatcacgcag
     3661 gaattgccag ccagagactg ctgaaacgga tttttttcat actttcggaa aggcagaaga
     3721 cggaaccgga gcggaaaaac agaaattcag gggaatgagc gcgatctggc aataacggca
     3781 aaacacagca acaaagacac accagaatcg cgcccatatg cgttttagtg cgttcccggt
     3841 gccccgaacc aaccattatc ccaaaaaatg caccggaaac acgctgacaa cattttagcc
     3901 tgtctctggt taccatccca gcgaagggcc atccagtgtc cgttcctgta tttccggctg
     3961 gcgctcacgc tccagggata actcatgttc gcgttcctgt atcagccgtt cgtcccttat
     4021 ctcctgttct cgctgtataa ctggctcaag cgttctgtcc gctcgctcaa gtgctgcacc
     4081 tgctgactca acttcatgac ccgctcgttc agcatcgcgt tgtcccgttg cgtaagcgaa
     4141 aacattttct gcaattccac gaaggcgctc tcccattcgt tcagccgctg catatagtcc
     4201 tgttgcagtt gctctaatgc gttcagcaaa tgtttttcca gctccgtcat gctctgtcac
     4261 tcctgtacca atctcactgc gttcagtttg ccgttgtcct ccgtccagtt cgtttcggct
     4321 ttcatcccct tcggcaacac cagaaaacgc ccgttgctgt cctcgtgata acgcacgccc
     4381 catgtcttcg cgttcagctt ctccagactc tctttctgct cgcggagaat attcatgttt
     4441 acgtttatct tcatgccctg ataccacagc agactcccgg tcgtggcaaa catcagcact
     4501 gagacgataa tcagccacag ccagcgtttc acgctcattt tcagtacggt ttgccaatgt
     4561 gtatccatcg tgctgacggt cgttttctga tatctctcca gcagttcttt cagggctgtt
     4621 gtatggtcgt tgatagcgtt cctgattaaa gattcgtttg aggtcagttc cctgtttaca
     4681 gattcttcga gcttcctgaa ctcgtcgttc agcattttct ctgtagattc ggctctctct
     4741 ttcagctttt tctcgaactc cgcgcccgtc tgtaaaagat tgctcataaa acgctccttt
     4801 cagtctgata ttcttcccgc cgttcgggtc tgcaatgctt atactgcttc gcgtcaccct
     4861 gaccacttca agccccgcct ccgtgagcgc ctgaattaca tcctgacggc cttttatctc
     4921 cccgacatga taaagagcgt ctatcccgcg tgtgacgctc tctgcaagcg cctgtttcgt
     4981 ttccggcagg ttatcaggga gagtcagtat ccggcggttc tccggggcgt tggggtcatg
     5041 cagcccgtaa tggtgattaa ccagcgtctg ccaggcatca attctcggcc tgtctgcccg
     5101 gtcgtagtac ggctggaggc gttttccgct ctgtagctcc atgttcggaa tgacaaaatt
     5161 cagctcaagc cgtcccttgt cctggtgctc cacccacagg atgctgtact gatttttttc
     5221 gagaccgggc atcagtacac gctcaaagct cgccatcact ttttcacgtc ctcccggcgg
     5281 cagctccttc tccgcgaacg acagaacacc tgacgtgtat ttcttcgcaa atggcgtggc
     5341 atcgatgagt tcccggactt cttccggatt accctgaagc accgttgccc cttcccggtt
     5401 acgctccctt cccagcaggt aatcaaccgg accactgcca ccaccttttc ccctggcatg
     5461 aaatttaact atcatcccgc gtcccctgtt ccctgacggc cagacgcagc cggcgcagct
     5521 catccccgat ggccatcagt gcggccacta cctgaacccg gtcaccggaa gaccactgtc
     5581 cgctgttcac cttcctcgct gtctgattca ggttattccc gatggcggcc agctgacgca
     5641 gtaacggcgg tgccagtgtc ggcagcttcc cggaacgggc aaccggctcc cccaggcaga
     5701 cccgccgcat ccataccgcc agttgtttac cctcacagcg ttccagtaac cgggcatgtt
     5761 catcatcagt aacccgtatc gtgagcatcc tctcgcgttt catcggtatc attaccccca
     5821 tgaacagaaa tcccccttac acggaggcat cagtgactaa acaggaaaaa accgccctta
     5881 acatggcccg cttcatcaga ggccagacat taacgctgct ggagaaactc aacgaactgg
     5941 acgcggatga acaggcagat atctgtgaat cgcttcacga ccacgccgat gagctttacc
     6001 gcagctgcct cgcacgtttc ggggatgacg gtgaaaacct ctgacacatg cagctcccgg
     6061 agacggtcac agcttgtctg tgagcggatg ccgggagccg acaagcccgt cagggcgcgt
     6121 cagcgggttt tagcgggtgt cggggcgcag ccatgaccca gtcacgtggc gatagcggag
     6181 tgtatactgg cttacttatg cggtatcagt gcgaattgta tgaaaagtgc accatgcagg
     6241 gtgtgaaatg ccgcacagat gcgtaaggag aaaatgcagc ttccggcgct cttccgcttc
     6301 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggttc tgactcacac
     6361 aaaaacggta gcacggttat ccacagaatc aggggataag gccggaaaga acatgtgagc
     6421 aaaagaccag tgcaggaaga aggccacgcc gctggcgttt ttccataggc tccgcccccc
     6481 tgacgagcat cacaaaatcg acactcaagt cagaggtggc gaaacccgac aggactataa
     6541 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgcca
     6601 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     6661 cgctgttggt atctcggctt ggtgtaggtc gttcgctcca agctgggctg tatgcacgaa
     6721 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtctcga gtccaacccg
     6781 gtaagacacg acttaacgcc actggcagca gccactg
//
