LOCUS       NZ_CP055117             6668 bp    DNA     circular CON 30-APR-2024
DEFINITION  Shigella flexneri strain SWHIN_101 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP055117
VERSION     NZ_CP055117.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13925596
            Assembly: GCF_022354705.1
KEYWORDS    RefSeq.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6668)
  AUTHORS   Che,Y., Xu,X., Yang,Y., Brinda,K., Hanage,W., Yang,C. and Zhang,T.
  TITLE     High-resolution genomic surveillance elucidates a multilayered
            hierarchical transfer of resistance between WWTP- and
            human/animal-associated bacteria
  JOURNAL   Microbiome 10 (1), 16 (2022)
   PUBMED   35078531
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6668)
  AUTHORS   Che,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2020) Civil Engineering, The University of Hong
            Kong, Pokfulam Road, Hong Kong 000000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: "Other" v. Unicycler
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology :: Illumina; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022354705.1-RS_2024_04_30
            Annotation Date                   :: 04/30/2024 04:20:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,096
            CDSs (total)                      :: 4,977
            Genes (coding)                    :: 4,761
            CDSs (with protein)               :: 4,761
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 216
            CDSs (without protein)            :: 216
            Pseudo Genes (ambiguous residues) :: 0 of 216
            Pseudo Genes (frameshifted)       :: 80 of 216
            Pseudo Genes (incomplete)         :: 139 of 216
            Pseudo Genes (internal stop)      :: 42 of 216
            Pseudo Genes (multiple problems)  :: 44 of 216
            Pseudo Genes (short protein)      :: 2 of 216
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP055117.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6668
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /strain="SWHIN_101"
                     /isolation_source="Shek Wu Hui WWTPs_101 WWTPs influent"
                     /db_xref="taxon:623"
                     /plasmid="unnamed4"
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.5 N 114.1 E"
                     /collection_date="2018-12"
     gene            complement(join(5205..6668,1..105))
                     /gene="cea"
                     /locus_tag="HUZ81_RS25350"
                     /old_locus_tag="HUZ81_25095"
     CDS             complement(join(5205..6668,1..105))
                     /gene="cea"
                     /locus_tag="HUZ81_RS25350"
                     /old_locus_tag="HUZ81_25095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WP_000447006.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            365..802
                     /locus_tag="HUZ81_RS25355"
                     /old_locus_tag="HUZ81_25100"
     CDS             365..802
                     /locus_tag="HUZ81_RS25355"
                     /old_locus_tag="HUZ81_25100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001405929.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     gene            883..1302
                     /locus_tag="HUZ81_RS25360"
                     /old_locus_tag="HUZ81_25105"
     CDS             883..1302
                     /locus_tag="HUZ81_RS25360"
                     /old_locus_tag="HUZ81_25105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(1620..1874)
                     /locus_tag="HUZ81_RS25365"
                     /old_locus_tag="HUZ81_25110"
     CDS             complement(1620..1874)
                     /locus_tag="HUZ81_RS25365"
                     /old_locus_tag="HUZ81_25110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015059032.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1881..2399)
                     /locus_tag="HUZ81_RS25370"
                     /old_locus_tag="HUZ81_25115"
     CDS             complement(1881..2399)
                     /locus_tag="HUZ81_RS25370"
                     /old_locus_tag="HUZ81_25115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067500.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<2437..3135)
                     /locus_tag="HUZ81_RS25600"
                     /old_locus_tag="HUZ81_25120"
                     /pseudo=""
     CDS             complement(<2437..3135)
                     /locus_tag="HUZ81_RS25600"
                     /old_locus_tag="HUZ81_25120"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3077..3400)
                     /locus_tag="HUZ81_RS25380"
                     /old_locus_tag="HUZ81_25125"
     CDS             complement(3077..3400)
                     /locus_tag="HUZ81_RS25380"
                     /old_locus_tag="HUZ81_25125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3464..3655
                     /locus_tag="HUZ81_RS25385"
                     /old_locus_tag="HUZ81_25130"
     CDS             3464..3655
                     /locus_tag="HUZ81_RS25385"
                     /old_locus_tag="HUZ81_25130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4325..4483
                     /locus_tag="HUZ81_RS25605"
     CDS             4325..4483
                     /locus_tag="HUZ81_RS25605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531517.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     gene            4981..5208
                     /locus_tag="HUZ81_RS25390"
                     /old_locus_tag="HUZ81_25140"
     CDS             4981..5208
                     /locus_tag="HUZ81_RS25390"
                     /old_locus_tag="HUZ81_25140"
                     /inference="COORDINATES: protein motif:HMM:NF015489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WP_229471606.1"
                     /translation="MLYAIVISTILCPYSKYAIEYIAFNFIKKDFFERRKNLNNAPVAK
                     LNLFMLYNLLCLVLAIPFGLLGLFISIKNN"
                     /name="colicin E1 immunity protein"
     Typing          complement(3741..3828)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          3839..4397
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.544"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1517..3087)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.809"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gccagagcca ctcccgtcag gagtaccatt caaaagagta ataattacct gtcccttatc
       61 atcataagga acaccatctt tatagtacgc taccgcggtt tccattataa aatcctcttt
      121 gacttttaaa acaataagtt aaaaataaat actgtacata taaccactgg ttttatatac
      181 agcataaaag ctacgccgct gcattttccc tgtcaagact gtggacttcc atttttgtga
      241 aaacgatcaa aaaaacagtc tttcacacca cgcgctattc tcgcccgatg ccacaaaaac
      301 cagcacaaac attaccgttc tcagacctca ttatgtttta ctgaaactat gagatgagac
      361 atctatggga cactgtcact ttatggcatg gcacacactc cgggacgcac taaaaatgac
      421 aggcagatcg cgttcacagt tttaccgtga tatgcgcgca ggccttgtca gttaccgtac
      481 cggcagggac ggacgacggg agtttgaaac cagtgaactg atccgggcat acggcgaatt
      541 aaagcagaat gagacaccag aaaggcacag tgagggacat gcagaaaatc cacatgatca
      601 gcagacagaa cgcattctcc gggagctgaa tgagctgaaa caatgcctga cgctgatgct
      661 tgaggataaa caggcacagg atatggatcg cagacgccag gaagcagaac gggaacagct
      721 acaaaatgag atagcccagc tcaggcaggc actggaactg gaaaagaaac ggggattctg
      781 gtccaggttg ttcggtcgct gaacgctgtc agagactgat gataaaatag tcttcggata
      841 ataactcacc gagaataaat actttaaggt agggagacac tcatgagacg taccggaaac
      901 aaactttgtc ttatcgccat gataacagca acagtagctc tcacagcctg taccccaaag
      961 ggcagcgtgg aacaacatac ccggcattac gtatatgctt ctgatgacgg ttttgatccc
     1021 aacttttcca cccaaaaagc cgacacaaca cgaatgatgg tgcctttttt tcggcagttc
     1081 tgggatatgg gagctaaaga taaagcgaca ggaaaatcac ggagtgatgt gcaacaacgc
     1141 attcagcagt ttcacagcca ggaattttta aactcactcc ggggcacaac tcaatttgcg
     1201 ggtactgatt accgcagcaa agaccttacc ccgaaaaaat ccaggctgct ggctgacacg
     1261 atttctgcgg tttatctcga tggctacgag ggcagacagt aagtggattt accataatcc
     1321 cttaattgta cgcaccgcta aaacgcgttc agcgcgatca cggcagcaga caggtaaaaa
     1381 tggcaacaaa ccacccgaaa aactgccgcg atcgcgcctg ataaatttta accgtatgaa
     1441 tacctatgca accagagggt acaggccaca ttacccccac ttaatccact gaagctgcca
     1501 tttttcatgg tttcaccatc ccagcgaagg gccatccagc gtgcgttcct gtatttccgg
     1561 ctgacgctcc cgttccaggg ataacacatg ttcgcgctcc tgtatcagcc gttcctctct
     1621 tatctccagt tctcgctgta taactggctc aagcgttctg tctgctcgct caagcgttct
     1681 gtctgctcgc tcaagtgctg cacctgctga ctcaactgca tgacccgctc gttcagcatc
     1741 gcgttgtccc gttgcgtaag cgaaaacatc ttctgcaatt ccacgaaggc gctctcccat
     1801 tcgctcagcc gctgcatata gtcctgttgc agctgctcta aggcgttcag caaatgtgtt
     1861 tccagctctg tcactctgtg tcactccttc agatgtaccc actctttccc ctgaaaggga
     1921 atcacctccg ctgatttccc gtacggaagg acaaggaatt tcctgttccc gtcctgcaca
     1981 aactccacgc cccatgtctt cgcgttcagt ttctgcaatg tctcttcctg cttcctgatt
     2041 tcttccaggt tcgcctgtat cctccctcca agataccaga gcgtcccgcc actcgcggta
     2101 aacaggagaa agactatccc cagtaacatc atgcccgtat tccctgccag ctttaacacg
     2161 tccctcctgt gctgcatcat cgcctctttc accccttccc ggtgtttttc cagcgattcc
     2221 tctgtcgagg ctgtgaacag ggctatagcg tctctgattt tcgtctcgtt tgatgtcaca
     2281 gcctcgctta cagattcgcc gagcctcctg aactcgttgt tcagcatttt ctctgtagat
     2341 tcggctctct ctttcagctt tttctcgaac tccgcgcccg tctgcaaaag attgctcata
     2401 aaatgctcct ttcagcctga tattcttccc gccgttcgga tctgcaatgc tgatactgct
     2461 tcgcgtcacc ctgaccactt ccagccccgc ctcagtgagc gcctgaatca catcctgacg
     2521 gccttttatc tctccggcat ggtaaagtgc atctatacct cgcgtgacgc cctcagcaag
     2581 cgcctgtttc gtttcaggca ggttatcagg gagtgtcaac gtcctgcggt tctccggggc
     2641 gttcgggtca tgcagcccgt aatggtgatt caccagcgtc tgccaggcat caattctagg
     2701 cctgtctgcg cggtcgtagt acggctggag gcgttttccg gtctgtagct ccatgttcgg
     2761 aatgacaaaa ttcagctcaa gccgtccctt gtcctggtgc tccacccaca ggatgctgta
     2821 ctgatttttt tcgagaccgg gcatcagtac acgctcaaag ctcgccatca ctttttcacg
     2881 tcctcccggc ggcagctcct tctccgcgaa cgacagaaca ccggacgtgt atttcttcgc
     2941 aaatggcgtg gcatcgatga gttcccggac ttcttccgga ttaccctgaa gcaccgttgc
     3001 gccttcgcgg ttacgctccc tccccagcag gtaatcaacc ggaccactgc caccaccttt
     3061 tcccctggca tgaaatttaa ctatcatccc gcgccccctg ttccctgaca gccagacgca
     3121 gccggcgcag ctcatccccg atggccatca gtgcggccac cacctgaacc cggtcaccgg
     3181 aagaccactg cccgctgttc accttacggg ctgtctgatt caggttattt ccgatggcgg
     3241 ccagctgacg cagtaacggc ggtgccagtg tcggcagttt tccggaacgg gcaaccggct
     3301 cccccaggca gacccgccgc atccataccg ccagttgttt accctcacag cgttcaagta
     3361 accgggcatg ttcatcatca gtaacccgta ttgtgagcat cctctcgcgt ttcatcggta
     3421 tcattacccc atgaacagaa atccccctta cacggaggca tcagtgacta aacaggaaaa
     3481 aaccgccctt aacatggccc gctttatcag aagccagaca ttaacgctgc tggagaagct
     3541 caacgaactg gacgcagatg aacaggccga tatttgtgaa tcgcttcacg accacgccga
     3601 tgagctttac cgcagctgcc tcgcacgttt cggggatgac ggtgaaaacc tctgacacat
     3661 gcagctcccg gagacggtca cagcttgtct gtgagcggat gccgggagct gacaagcccg
     3721 tcagggcgcg tcagcaggtt ttagcgggtg tcggggcgca gccctgaccc agtcacgtag
     3781 cgatagcgga gtgtatactg gcttaaccat gcggcatcag tgcggattgt atgaaaagta
     3841 cgccatgccg ggtgtgaaat gccgcacaga tgcgtaagga gaaaatgcac gtccaggcgc
     3901 ttttccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg gcgagcggta
     3961 ctgactcaca caaaaacggt aacacagtta tccacagaat caggggataa ggccggaaag
     4021 aacatgtgag caaaagacca ggaacaggaa gaaggccacg tagcaggcgt ttttccatag
     4081 gctccgcccc cctgacgagc atcacaaaaa tagacgctca agtcagaggt ggcgaaaccc
     4141 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     4201 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     4261 ttctcatagc tcacgctgtt ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     4321 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     4381 tgagtccaac ccggtaaggc acgccttaac gccactggca gcagccactg gtaaccggat
     4441 tagcagagcg atgatggcac aaacggtgct acagagttct tgaagtagtg gcccgactac
     4501 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     4561 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ttggtagcgg tggttttttt
     4621 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     4681 tctactgaac cgcgatcccc gtcagtttag aagaggagga tggtgcgatg gtccctccct
     4741 gaacatcacg tatatagtta gcctgacatc caacaaggag gtttatcgcg aatattccca
     4801 caaaaaatct tttcctcata actcgatcct tataaaatga aaagaatata tggcgaggtt
     4861 taatttatga gcttaagata ctacataaaa aatattttat ttggcctgta ctgcacactt
     4921 atatatatat accttataac aaaaaacagc gaagggtatt atttccttgt gtcagataag
     4981 atgctatatg caatagtgat aagcactatt ctatgtccat attcaaaata tgctattgaa
     5041 tacatagctt ttaacttcat aaagaaagat tttttcgaaa gaagaaaaaa cctaaataac
     5101 gcccccgtag caaaattaaa cctatttatg ctatataatc tactttgttt ggtcctagca
     5161 atcccatttg gattgctagg actttttata tcaataaaga ataattaaat ccctaacacc
     5221 tcatttatag tattaagttt attcttatca atataggagc atagaattcc tgtaacaata
     5281 gcaatacccc aaatacctaa tgtagttcca gcaagcaagc taaaaagtaa agcaacaaca
     5341 taactcaccc ctgcatctgc agctttcttc tctaatgtaa gaaatagtgg cttccagtca
     5401 cctgtatcct taatttttag gatatcagat accacatcat atccaaaaga aacatgcccc
     5461 gtaatcttca agtacttggc aaactgatct aaatgtttag cccagtcatc atacttcacc
     5521 gatgccaacg cattaaaaat agcatcacga tcggctttgc tgaatttctt atttaaaaca
     5581 tccttgtatt tttcaaaagc agcgagagct tcattcacat tgccgatttt cttaccttta
     5641 gacttatcag caagttcctg tgccattttc gaatattttt caccatattt ttcagtcagc
     5701 gtttgataaa agctaactgt tgcatcaaca gcatccttaa tctgtgaatt aaggagatta
     5761 ttctgtgctt ttttcaaatt ttcttcagct tcatgaacac gagcgatacc ggcattacga
     5821 ttattactga cctgagaaat agccttctgg atctgagtta tatcagcatt tatccggtta
     5881 atacgtgttt ctgatgctgt tacctgtttt tgtttttctt ctctaatctt accggcccca
     5941 acccgtcgtc tggttgcttc aaaaaaagga cggttctgaa gcggatcatt ggctcttggt
     6001 gatagttttt tgaccagctc atccagttct ttatatttag cggatgcctg agccagttca
     6061 tttcgttttc cagcgagcgt tttcatttct gcatcacggg catggatact ggagcttaaa
     6121 cgagaattga gagtcttaat ctctccatcc attttcacca cttcagattg tgcagcagaa
     6181 agtttttttt gggcgatctc aacagcttta gcttcttcac tcaatgcagc cagtcgtttc
     6241 tcttcagctt cagccagttt caactggcgt tctgtttcag ccttctcccg ttcaatctct
     6301 ttacgtcgtt gttctgcttc ctgaaaagcc ttttctgctg cttccgcttc tttacgggct
     6361 ttttcttctg ctttcgcaag gcgcaaacgc tctgcttccg cctgcatagc tgcattatta
     6421 gcatgagcaa gctctgttgc tgaaggcgta cgtgaggcat tgtgacgaag agcctcattc
     6481 acgatatcct tcaggcgctg agtcagcgca tccctgtttg cctttgcttt cgcctgtgct
     6541 tccgctgcag cttttgcccg ggcagcctgc tctgcctgtg ttttctttaa ttgagcagta
     6601 gaccatttag cagttgcatg aatagctgca gaactttcac ttttactgcc tccttttcca
     6661 cctccgcc
//
