LOCUS       NZ_CP055112             8758 bp    DNA     circular CON 30-APR-2024
DEFINITION  Shigella flexneri strain SWHIN_104 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP055112
VERSION     NZ_CP055112.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13925597
            Assembly: GCF_022354685.1
KEYWORDS    RefSeq.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8758)
  AUTHORS   Che,Y., Xu,X., Yang,Y., Brinda,K., Hanage,W., Yang,C. and Zhang,T.
  TITLE     High-resolution genomic surveillance elucidates a multilayered
            hierarchical transfer of resistance between WWTP- and
            human/animal-associated bacteria
  JOURNAL   Microbiome 10 (1), 16 (2022)
   PUBMED   35078531
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8758)
  AUTHORS   Che,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2020) Civil Engineering, The University of Hong
            Kong, Pokfulam Road, Hong Kong 000000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: "Other" v. Unicycler
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology :: Illumina; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022354685.1-RS_2024_04_30
            Annotation Date                   :: 04/30/2024 04:25:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,949
            CDSs (total)                      :: 4,829
            Genes (coding)                    :: 4,658
            CDSs (with protein)               :: 4,658
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 171
            CDSs (without protein)            :: 171
            Pseudo Genes (ambiguous residues) :: 0 of 171
            Pseudo Genes (frameshifted)       :: 64 of 171
            Pseudo Genes (incomplete)         :: 108 of 171
            Pseudo Genes (internal stop)      :: 24 of 171
            Pseudo Genes (multiple problems)  :: 25 of 171
            Pseudo Genes (short protein)      :: 3 of 171
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP055112.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8758
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /strain="SWHIN_104"
                     /isolation_source="Shek Wu Hui WWTPs_104 WWTPs influent"
                     /db_xref="taxon:623"
                     /plasmid="unnamed3"
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.5 N 114.1 E"
                     /collection_date="2018-12"
     gene            complement(join(6902..8758,1..1032))
                     /locus_tag="HUZ82_RS24630"
                     /old_locus_tag="HUZ82_24415"
     CDS             complement(join(6902..8758,1..1032))
                     /locus_tag="HUZ82_RS24630"
                     /old_locus_tag="HUZ82_24415"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0003896 - DNA primase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="WP_167775590.1"
                     /translation="MARIPEAELQHLKAAVALVAVVQAQGRQLVKKGKDFTTLCPFHDE
                     KTPSCVISPEKNLYHCFGCNAGGSVLNWVMQTEKLSLRKAVERLKAELGDNPAVVPLVT
                     KDEPEIFAEDEAGRQKLLSRVVEFYHHTLLNAPEAVAYLEKRRLNHPELVAAFKLGFAN
                     RTLPYRLPAVKSRAGEKIRARLKGVGVLRESGHEHFTGSLVVPVMDLNGQIREMYGRKI
                     GGDLRKGTPLHMYLPGTHGGVWNEQALIGSSSVVLCESLIDAMSFWVAGVRNVTAAYGV
                     NGFTDEMRAALLAHGVKQVLIAYDNDPAGMDANGYLCQVAEPEQAFSVLLDGAVPMSDL
                     ADVASIAPQASAEPAHLTSLAADVAALPAVSAPAPGVSVETLADGELMVALPGERWTIR
                     GVSRKTNAAAMKVNAQVLDTESGVVFADAVDMMSARSRGGYARAAAAELGLAEGDLRRS
                     LGKVLLALEHWQAAPEPANAAPEMTPEDREAALELLRSPDLASRIAADMAACGVVGEST
                     NLTAAYLAAVSRKLDRPLAVLIQSSSAAGKSSLMDAVLNLIPPEERLQYSAMTGQSLFY
                     LGETNLQHKILAIAEEEGVRQAAYALKLLQSDGELTIASTGKDDASGNLVTKQYTVKGP
                     VMLMLTTTAIDVDEELLNRCLVLTVNESREQTEAIHAAQRYAQTLEGLLAANEKSYITQ
                     LHQNAQRLLRPLNVVNPFASQLTFMSDKTRTRRDHMKYLTLIQTIALLHQYQREIKRVE
                     HRGQVVEYIEVTREDIALANRLAHEILGRTLDEMPPQTRKLLLLIQTMVGELAAAQHCK
                     PGEVRFTRREIRDFTQWSDNQLKVHCMRLAEMEYLLIHGGSRGHLLQYELLWDGSGDGS
                     HLCGLIEPEDSPVYDSGKLGQNADRLPLSCPHVGAKLGAVKPPVSRASTGTDAPQVGAD
                     EKTLFPTARKTSHRNSNTPESRPTPHDGAPAV"
                     /name="DNA primase"
     gene            1147..1539
                     /locus_tag="HUZ82_RS24635"
                     /old_locus_tag="HUZ82_24420"
     CDS             1147..1539
                     /locus_tag="HUZ82_RS24635"
                     /old_locus_tag="HUZ82_24420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316688.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_001724481.1"
                     /translation="MAVLTTGQLQQAMNTKDEQFFKQLGARIAQARKEQQLTQTQLAEQ
                     LGIAQQTMAHYEGGKLKVSAALLPQLAQILNLSLDELLGLPAPRRSSKRGPASRLEQQI
                     EIISQLPRTKQKLVSEILDNVIGKTQ"
                     /name="helix-turn-helix transcriptional regulator"
     gene            1621..1914
                     /locus_tag="HUZ82_RS24640"
                     /old_locus_tag="HUZ82_24425"
     CDS             1621..1914
                     /locus_tag="HUZ82_RS24640"
                     /old_locus_tag="HUZ82_24425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807559.1"
                     /GO_component="GO:0005737 - cytoplasm [Evidence IEA]"
                     /GO_function="GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]; GO:0003723 - RNA binding
                     [Evidence IEA]"
                     /GO_process="GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
                     /protein_id="WP_001401851.1"
                     /translation="MAERDITPEVAISKTQRRCTVGYLPNRSDTPVIKISAKWLREAGF
                     EIGTGVTVKISEGCLILLADNNEVQELRRELYQVKQSVKGMRDGMFSVLNES"
                     /name="SymE family type I addiction module toxin"
     gene            complement(1961..2425)
                     /locus_tag="HUZ82_RS24645"
                     /old_locus_tag="HUZ82_24430"
     CDS             complement(1961..2425)
                     /locus_tag="HUZ82_RS24645"
                     /old_locus_tag="HUZ82_24430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023155272.1"
                     /translation="MEKLQNAEGNKMITGVDYVFYSNKKPFDIEEDFVSLLKMKWRECI
                     IDEFERTDSRLDLFFAKDKEMYSLFDEIGYSLNDHGEGCFMLVSSMLNRLESKIKVLDV
                     IYPENKRNTEQYDSIIMLRDIWEYTLVLPSEIAESDFSRDIYTYLENVLR"
                     /name="hypothetical protein"
     gene            complement(2394..>3221)
                     /locus_tag="HUZ82_RS24650"
                     /old_locus_tag="HUZ82_24435"
                     /pseudo=""
     CDS             complement(2394..>3221)
                     /locus_tag="HUZ82_RS24650"
                     /old_locus_tag="HUZ82_24435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465819.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /name="RHS repeat-associated core domain-containing
                     protein"
     gene            complement(3233..4369)
                     /locus_tag="HUZ82_RS24655"
                     /old_locus_tag="HUZ82_24440"
     CDS             complement(3233..4369)
                     /locus_tag="HUZ82_RS24655"
                     /old_locus_tag="HUZ82_24440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="WP_032151703.1"
                     /translation="MELKKLMEHISIIPDYRQAWKVEHKLSDILLLTICAVISGAEGWE
                     DIEDFGETHLDFLKQYGDFENGIPVHDTIARVVSCISPAKFHECFINWMRDCHSSDDKD
                     VIAIDGKTLRHSYDKSRRRGAIHVISAFSTMHRLVIGQIKTDEKSNEITAIPELLNMLD
                     IKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNPEHDS
                     YAISEKSHGREEIRLHNVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEMTV
                     RYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGSAAELFSGIRHIA
                     INILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
                     /name="ISAs1-like element ISEc1 family transposase"
     gene            complement(4448..4627)
                     /locus_tag="HUZ82_RS24660"
     CDS             complement(4448..4627)
                     /locus_tag="HUZ82_RS24660"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_198131273.1"
                     /translation="MGLRGGLNLYAYAPNPLSWIDPFGLSGVGIPYGFKSYGQGKIIRS
                     SLFVLNNSHFMFKN"
                     /name="hypothetical protein"
     gene            complement(5592..6701)
                     /gene="xerC"
                     /locus_tag="HUZ82_RS24665"
                     /old_locus_tag="HUZ82_24450"
     CDS             complement(5592..6701)
                     /gene="xerC"
                     /locus_tag="HUZ82_RS24665"
                     /old_locus_tag="HUZ82_24450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerC"
                     /protein_id="WP_032286024.1"
                     /translation="MTKPKSPGPEALYLGRQNEHTTQRQHMLAWLESLEAAGYSARSVE
                     GYRERLLPFMTWCEERGLLYAPQISLAVLEAYQRHLRSYRKADGNTLAVGGQLSRLSAV
                     KNFFRWLLKRHVILYSPAEMLTLPKEEKRLPAQVFSEQETRQVLQSLDTEKPLGLRNRA
                     ILEVLWSTGIRRMEVANLMLSDVDFTRGVVNVRQGKGKKDRVVPVGHVALEWVTRYLRN
                     VRPHLTQRADSRHLFVTYHGKGLNRGTLTQIGGETIREKARIQKPGACHIFRHSMATQM
                     LDNGADTRHIQAILGHEKLETTQVYTRVAIGHLQEVHALTHPEEQDGTWTDRHGSASDA
                     VAAEPQTRRERNHAEMERALVSRKRSRRR"
                     /name="xerC"
ORIGIN
        1 cgccacgtca gccaggtcac tcatgggcac cgcgccgtcc agcaacacac tgaacgcctg
       61 ttccggctct gccacctggc acagataacc gttcgcatcc atgcccgccg gatcgttgtc
      121 gtaggcgatc agtacctgct taacgccgtg tgccagcagt gcggcccgca tctcatcagt
      181 aaagccgttc accccgtaag ccgccgtgac gttacggacc ccggcgaccc agaacgacat
      241 ggcgtcgatc agggattcgc acaggacaac cgacgatgac ccgatcagcg cctgctcgtt
      301 ccacacaccg ccgtgagtgc cgggcaggta catatgcagc ggcgttccct tacgcagatc
      361 gccgccgatc ttgcgcccgt acatctcgcg gatctgcccg ttcagatcca tcaccggcac
      421 aaccagcgac ccggtgaagt gttcatgccc ggactcacgc agcacaccca cacccttaag
      481 ccgggcgcgg atcttctcgc ccgcccgtga tttaacggcg ggcagacggt aaggcagggt
      541 acggttggcg aagcccagct taaaggcagc gaccagttca ggatggttaa gacggcgctt
      601 ttccagatac gccaccgctt ccggcgcgtt cagcagcgtg tggtggtaga actccaccac
      661 gcgggagagc agcttctggc ggcccgcttc atcttccgcg aagatttccg gttcatcctt
      721 cgtgaccagc ggcaccacgg caggattgtc gcccagctcc gctttcagcc gctccaccgc
      781 cttacgcagg ctgagttttt ccgtctgcat cacccagttc agcaccgatc cgcccgcgtt
      841 acagccgaag cagtgataca ggtttttctc cggcgagatc acacaggacg gcgttttctc
      901 gtcatggaac gggcacaacg tggtgaagtc cttccccttc ttaaccagtt gtcgcccctg
      961 cgcctgaacc acggcgacta aggcgacggc ggctttcagg tgctgcaact ctgcttccgg
     1021 gatgcgggcc ataacgggaa ctcctgtaaa aacctgtcaa tagacttagg gtaaaaataa
     1081 ctctgttgca gagtactcta tattagggta ttgtcaacca agttatttaa aacagggagt
     1141 aacatcatgg cagtgttaac gacaggacag ctacagcagg ccatgaacac caaagacgaa
     1201 cagtttttta agcagcttgg cgcacggatc gcacaggcac gtaaggaaca gcaactgaca
     1261 cagacgcaac tggcagaaca gttagggatt gcacagcaga cgatggcgca ttacgaaggc
     1321 gggaagctca aggtttcggc agcgctgtta ccacaactgg cacagatcct gaacctgtcg
     1381 ctggatgaac tgctcggatt gccagcacca cgcagaagca gcaagcgcgg cccggcgtca
     1441 cggctggaac agcagatcga gatcatcagc cagttaccgc gaacgaagca gaagctggtt
     1501 tctgagattt tagataatgt gataggaaaa acgcagtaat aacattgaga tagcgcgatg
     1561 attaagggct gccaccctta accaccgctc accataagca actaacagag gtagttaatc
     1621 atggctgaac gcgatattac gccagaagtc gccatttcca aaacacagcg tcgctgtacc
     1681 gtgggttatc tgcccaaccg cagcgacacg ccggtgatca aaatcagcgc caaatggctg
     1741 agagaagcgg gatttgagat cggcacgggc gttaccgtga agatctcgga aggctgcctg
     1801 atcctgcttg cggataacaa cgaagtacag gaactgcgca gggaacttta tcaggttaaa
     1861 cagtcagtta aggggatgcg tgacgggatg tttagtgtgc tgaacgagag ttaaataata
     1921 aaaaagccgg gaagatttga tattctttcc ggctttttat ttatcttaaa acattttcga
     1981 gataagtata aatatccctg gagaaatcac tttcagcaat ttctgaaggt aataccaaag
     2041 tatattccca tatatcacgc aacataataa tagaatcata ttgttctgta ttacgtttat
     2101 tttctggata aatgacatcc aatactttta ttttactttc caagcggtta agcatgctcg
     2161 aaacaagcat aaaacaacct tcaccatgat catttagaga gtatcctatt tcatcaaaaa
     2221 gagagtacat ttctttatct tttgcaaaaa acagatccag tctagaatca gttctctcaa
     2281 actcatcaat aatgcattct ctccatttca tttttaataa agaaacaaaa tcctcttcaa
     2341 tatcaaatgg ttttttattg gagtaaaaaa cataatcgac gccagttatc atcttatttc
     2401 cctccgcatt ttggagtttt tccatgtctg gagacgttgt ttctatcaag atctaaatcg
     2461 ccctcaatat atctaataaa ttctccacga tcacccctaa taggtttcag caaagaatcg
     2521 tcgatttgaa attcaaaaaa atgtgttgat ttagttgatg tgagtcccaa ttttttaaat
     2581 ccattcggtg tcttagccaa ttcttcaggt gattttgttg tcacatatat accttttgga
     2641 tgacctttaa ttggactgga ggctttaaat ttataaggtt tctgagaaga aattccgtta
     2701 tatccacctt gatcagtata gtggtatacg gtaacaattt ttaagccgag aggatcgacc
     2761 caccccagag gattcggcgc ataagcatat aaatttaacc cacctaatag ccctatcggg
     2821 tccgttacca tgaaccgccc gacatccgga tcatagtacc tgtgcagatt gtagtgcagc
     2881 cccgtttccc tgtcgaggta ctgtccctgg tagcgcaggt tctgctccac cccgccgtgg
     2941 tccttctcct gtatcgtgtt accccagacc tgataccccg tctcccacac gattttcccg
     3001 tcactgtccg tcatctcttc cggcgcgccg ttcacatccg tgtgaaaata cagtatccgg
     3061 ttcgggccct ctttgcctgc cttatccacc cgcgccagcg gttcataact cccctggtcc
     3121 ctgtagatat acaggctgcc tgttccctcc ggcctgctct cctgcgccag ccgcattccg
     3181 tcccataaaa attttgtccg gttgtacggt ttaccgtcag ggcagggcaa gattacgaaa
     3241 gcccgctccc cgcaaggact gacgccagat agtttctgtc catggctgct tttcgcatct
     3301 tacgtcttaa ccctgccttg aataccttat cattcgtcaa aatattaata gcaatgtgcc
     3361 gtatccctga aaataattct gctgcacttc ctcttcttat tttgcagtcg tcttcattca
     3421 ttaccacgtc cagacgccag tgcagcttat tctccacgtg ccagtgattt cggatcgctg
     3481 tggcgaattt ctcagcggtt aaatcagcag aactgatata atatctgacc gtcatttctg
     3541 gctctttctt ttgttctgct attatggacc gaaaggagac tgccacgcat aatttcttca
     3601 gccctttcca ttcaaacgtg aaatcaataa gttcatcagg gacatcgcaa acattatgaa
     3661 gacggatttc ttctctgccg tgactctttt cactaattgc gtaactgtca tgctctggat
     3721 tatttaattc tttcagcgga aatttttcct caaaggcttt atttagccgc ccctggtttc
     3781 cttttaccgc gaataaataa tcacctccct gtttttgtat cttctctgca atatctttct
     3841 ggcaacccat cgcatcagtt gtgatgattt ttcctttaat atccagcatg ttaagaagtt
     3901 ctgggatagc tgtaatctca ttagatttct catccgtctt gatctgtccg atgaccagtc
     3961 tgtgcattgt tgagaacgca ctaatgacat gaatcgctcc cctgcggcga ctcttgtcat
     4021 aagagtgccg gagcgttttt ccatcaattg caatgacgtc tttatcatct gaagaatggc
     4081 agtcacgcat ccagttaata aagcactcgt gaaattttgc aggactgata caggatacaa
     4141 ctctggcaat ggtatcgtga acaggaatac cattttcaaa atcaccatat tgcttcaaaa
     4201 aatcgagatg tgtttcccca aaatcctcta tatcttccca accttccgca ccagaaataa
     4261 cggcacaaat agtcaacaat agaatatccg ataatttatg ttccactttc caggcttgtc
     4321 tgtaatcggg gataatagaa atatgttcca tcaatttttt aagttccatt ttgttctcct
     4381 taattatgta aggagtattt gatcatgtat aagcaataaa aaacagcttc aggtaataag
     4441 gaatatctca atttttaaac ataaaatgcg aattatttag tacaaaaagt gaacttctga
     4501 tgatctttcc ctgcccatag ctcttaaaac catacggtat gcctacacca gataagccaa
     4561 acggatcgat ccatgacagc ggattcggcg cataagcata taagtttaac ccgcctctca
     4621 gccctatcgg gtcatcggga cgatccggtg ggggtcacac tttttaacac gctaccgccc
     4681 gcgccgtttc tggcttcccg gctatcacct tttacttatt taacagcacg ttaacccagc
     4741 tttcccgacg cgcgttttca cgcccgctca ccatatcacc accaccacga acctgtcatt
     4801 ccgctttcca ctaagtccgt ttccggcacc ccgtgacggg gtgccgccag gccgccaccg
     4861 gcggcatggc ggggccacgg cgcggggtct gcgggtgcgg gcggcagaga gtccgcaagc
     4921 gacggaacgg cgctctgtgg caccgctgcc tgaccggagc tggcctttgc atgaccatgc
     4981 cggaccgggg cgggcagcgg aagctggcga ggcgggaacg agtgacgcgc ggcctttttg
     5041 cgcggcacga gccgccgcag ccagacgacg ccgggcgggc ataaagtcct tttaagcgga
     5101 ggccgccagg ccgacaacct cttttgactg actgccacac gatgacgccg ccgcagaaca
     5161 gcaccggagc cgccagggat ggcggtgact gcggggccac agaacggcac caccgctgac
     5221 agcagagcgg agcgccagcg aagctttgcg ctgcacggac tgcggggctg aacgcggggg
     5281 cgccagacat aatgcggatt gtgcgaactc gagccgcgcg cagcgggtcg ggttcgccgc
     5341 cgttgccacc cggcgagagt gcgcataatc ccccgcatta tgttgaatga cgtttgcccc
     5401 gtgctgctta gtcgcatgga gcgcggcagc ctccacgggg cggacggcat ttacccccgc
     5461 gcactccgat ggcagggggc agtcaaccga cagggaaggc ggttgtctgc cctgcacctc
     5521 accccacggc ctcacaggga tcgcccgcta acttttcagg ctgtccggcg acgcagttgc
     5581 ttcgcccggt gtcagcgtct ccggctgcgc ttcctgctga ccagcgcccg ctccatctcc
     5641 gcatggttgc gctcacgccg cgtctgcggc tctgccgcca ctgcgtcgct ggcgcttccg
     5701 tgccgatctg tccacgtccc atcctgctct tccgggtgcg tcagcgcgtg tacttcctga
     5761 agatgaccga tggcaacccg cgtgtagacc tgcgtcgtct ccagcttctc atgcccgagg
     5821 atcgcctgga tatgccgcgt gtccgcgccg ttgtccagca tctgtgtggc cattgagtgg
     5881 cggaagatgt ggcaggcgcc cggtttctgt atccgcgcct tttcacggat ggtttccccg
     5941 ccgatctgcg tcagtgttcc ccggttcagt cctttgccgt ggtacgtcac gaacaggtgt
     6001 ctactgtcgg cgcgttgcgt caggtgaggt cggacgttac gcaggtagcg cgttacccac
     6061 tccagcgcca catggcccac cggaacgacc cgatccttct tccccttgcc ctggcgcacg
     6121 ttcaccacgc cgcgcgtgaa gtccacgtct gacagcatca ggtttgccac ttccatgcgc
     6181 ctgatcccgg tgctccacag cacttccagt attgcccggt tacgcagccc cagcggcttc
     6241 tccgtgtcca gactctgtaa cacctgccgc gtttcctgct cgctgaacac ctgcgccgga
     6301 agccgtttct cctccttcgg cagcgtcagc atttctgccg gactgtagag gatgacgtgc
     6361 cgcttcagca gccagcggaa gaagttcttc actgcgctta agcggctaag ctgcccgccc
     6421 accgccagcg tgttaccgtc ggccttacgg tagctgcgaa gatggcgctg gtacgcttcc
     6481 agcaccgcca ggcttatctg tggcgcatac agcaggccgc gttcttcgca ccatgtcatg
     6541 aacggtaaca gccgttcgcg gtagccttcc acgctgcgcg cgctgtagcc tgcggcttcc
     6601 aggctttcca gccaggcgag catgtgctgg cgctgcgtcg tgtgttcgtt ctggcgacca
     6661 agataaaggg cttccggccc cggtgatttg ggctttgtca tgatgattgt cctgattgag
     6721 attacggtac gatggggata agaacagagg gtggaacagg ggttttcgtg ctgccccaac
     6781 ttgcggggcg ttcaggccag taatggcgcg gtctgaggga gattttcact cccccaactc
     6841 agccccaaca tgggggcaac ttaagggtaa cttgcgcctg gtcaggtcca acttgcggga
     6901 gtcataccgc tggcgcaccg tcatggggtg tcgggcggct ttccggcgta ttactgttac
     6961 gatgtgatgt tttcctggct gtcgggaaca gtgttttttc atctgctcca acttgcggcg
     7021 catcagtgcc agtactggcg cggcttacag gcggttttac cgcccccaac ttagccccaa
     7081 catgtgggca acttaagggc aacctgtccg cgttctgccc caacttgccg gagtcataca
     7141 ccgggctgtc ctccggttcg atcaggccgc acaggtgcga gccgtcgccg ctgccgtccc
     7201 acagcaactc gtattgcagc aggtgacccc ggctgccgcc gtggatcagc aggtattcca
     7261 tttccgccag ccgcatacag tggactttaa gctggttgtc gctccactgc gtgaagtcgc
     7321 ggatctcccg gcgcgtgaag cgtacctcgc cgggtttgca gtgctgcgcc gctgccagtt
     7381 caccgaccat tgtctggatc agcagcaaca gctttctggt ctgcggcggc atttcgtcca
     7441 gggtgcgccc gagtatttca tgtgccaggc ggttcgccag cgcgatgtct tcccgcgtca
     7501 cctcgatgta ctccaccacc tgcccgcgat gctccacacg tttgatctcc cgctgatact
     7561 ggtgcagcag ggcgatcgtc tggatcagcg tcaggtattt catgtggtcg cgccgggtgc
     7621 gggttttgtc gctcatgaac gtgagctgtg acgcgaacgg gttcaccacg ttgagcggac
     7681 gtaacagccg ctgcgcgttc tggtgaagct gagtgatgta acttttttcg ttagccgcca
     7741 gcagtccttc cagcgtctgc gcgtaacgct gcgccgcatg gatcgcttcc gtctgctccc
     7801 gcgactcgtt caccgtcagc accaggcaac ggttgagtaa ctcctcgtca acgtcgatgg
     7861 cggtggtggt cagcatcagc atcaccgggc cttttaccgt gtactgtttc gtgaccaggt
     7921 taccgctggc gtcgtcctta cccgttgacg cgatcgttaa ctcgccgtcc gactgcaaca
     7981 gcttgagcgc atacgccgcc tggcgcacac cttcttcttc cgcgatggcg aggatcttgt
     8041 gttgcaggtt ggtttcaccg aggtagaaca ggctctgccc ggtcatggcg ctgtactgta
     8101 agcgttcttc cggcgggatc aggttgagca ccgcatccat cagcgaggat ttgcccgccg
     8161 cgctcgatga ctggatcagc accgccagcg gcctgtccag cttacgcgac accgccgcca
     8221 gatacgccgc cgtcaggttg gtggactcac ccaccacacc acacgccgcc atgtctgccg
     8281 cgatgcgtga tgcaagatcc ggcgagcgca acaactcaag cgctgcctcc cgatcttccg
     8341 gcgtcatctc cggggcggca ttcgcgggct ccggtgcggc ctgccagtgc tccagcgcca
     8401 gcagcacctt acccagcgag cgacgcagat cgccttctgc caggccaagc tctgccgctg
     8461 cggcccgcgc ataaccgccg cgactgcggg cgctcatcat atccaccgca tcggcgaaca
     8521 ccacgccgct ttccgtgtcc agcacctgcg cgttaacctt catcgccgcc gcgttggttt
     8581 tgcggcttac gccccggatt gtccagcgtt cgcccggtaa ggctaccatc agttcgccat
     8641 cagccagggt ttccacgctg acgcccggcg caggcgcact aactgccgga agcgcggcaa
     8701 catcagcggc taacgaagtg aggtgtgcag gctccgcgct ggcctgcggc gcgatact
//
