LOCUS       NZ_CP055094            12602 bp    DNA     circular CON 01-MAY-2024
DEFINITION  Shigella flexneri strain SWHIN_110 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP055094
VERSION     NZ_CP055094.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13925602
            Assembly: GCF_022354585.1
KEYWORDS    RefSeq.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12602)
  AUTHORS   Che,Y., Xu,X., Yang,Y., Brinda,K., Hanage,W., Yang,C. and Zhang,T.
  TITLE     High-resolution genomic surveillance elucidates a multilayered
            hierarchical transfer of resistance between WWTP- and
            human/animal-associated bacteria
  JOURNAL   Microbiome 10 (1), 16 (2022)
   PUBMED   35078531
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12602)
  AUTHORS   Che,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2020) Civil Engineering, The University of Hong
            Kong, Pokfulam Road, Hong Kong 000000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: "Other" v. Unicycler
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology :: Illumina; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022354585.1-RS_2024_05_01
            Annotation Date                   :: 05/01/2024 05:10:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,453
            Genes (coding)                    :: 4,256
            CDSs (with protein)               :: 4,256
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 197
            CDSs (without protein)            :: 197
            Pseudo Genes (ambiguous residues) :: 0 of 197
            Pseudo Genes (frameshifted)       :: 60 of 197
            Pseudo Genes (incomplete)         :: 124 of 197
            Pseudo Genes (internal stop)      :: 45 of 197
            Pseudo Genes (multiple problems)  :: 31 of 197
            Pseudo Genes (short protein)      :: 3 of 197
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP055094.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12602
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /strain="SWHIN_110"
                     /isolation_source="Shek Wu Hui WWTPs_110 WWTPs influent"
                     /db_xref="taxon:623"
                     /plasmid="unnamed2"
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.5 N 114.1 E"
                     /collection_date="2018-12"
     gene            join(11180..12602,1..122)
                     /locus_tag="HUZ87_RS22690"
                     /old_locus_tag="HUZ87_22585"
     CDS             join(11180..12602,1..122)
                     /locus_tag="HUZ87_RS22690"
                     /old_locus_tag="HUZ87_22585"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="WP_201360865.1"
                     /translation="MKNYDVFYGGIFKTITDYKLLKIQKDIKNNMTALVSSFKWVGIGQ
                     LVKIATQLVSLFYLTHLIPPSSYGLLAMSSVVMNLASIFNDMGTGAAVIQRQEASIGFY
                     NYIYRINIFTGLFVMLLVIITSPLIVVYFNTPELYPVLFLLAFSFPISSLTITHKAKLE
                     KEMNFKVIIAIEIFSSLTGMVLAIVLANLGFGVYSLIFQTLTTISISTICFLKVSGLSL
                     SLRIKNISHSDKSGVIGFSGYLLSFNLINYFSRNLDSILIGRFFSSAILGVYSIAYRIM
                     LFPLQSLTLVFSRVFLPHLSKNINNTNNTNKQDYLNALKLILSLSAPLMLGLSATSFSF
                     TRIFFDPKWHLLGDLLHWLAPTAIIQSALSTTGSVFMAYGKTRWLFFLGCIGAFLMGLS
                     FIIGVHFDIKTLVYLYFFANLINSLPAFILLKNILKFSYIELVNVFFRTISPAFLMFLL
                     IKVWQHFSFFKNASIDFSFNVALGIFSYIVFYTLFNFNEVVRTGVIHKLRCMTKLK"
                     /name="lipopolysaccharide biosynthesis protein"
     gene            complement(<215..479)
                     /locus_tag="HUZ87_RS22695"
                     /old_locus_tag="HUZ87_22520"
                     /pseudo=""
     CDS             complement(<215..479)
                     /locus_tag="HUZ87_RS22695"
                     /old_locus_tag="HUZ87_22520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558268.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element transposase"
                     /name="IS1-like element transposase"
     gene            complement(624..1500)
                     /locus_tag="HUZ87_RS22700"
                     /old_locus_tag="HUZ87_22525"
                     /pseudo=""
     CDS             complement(624..1500)
                     /locus_tag="HUZ87_RS22700"
                     /old_locus_tag="HUZ87_22525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946055.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(1699..1983)
                     /gene="nadS"
                     /locus_tag="HUZ87_RS22705"
                     /old_locus_tag="HUZ87_22530"
     CDS             complement(1699..1983)
                     /gene="nadS"
                     /locus_tag="HUZ87_RS22705"
                     /old_locus_tag="HUZ87_22530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="WP_032242301.1"
                     /translation="MSFFDELKASLEEAVEIKNGTKAPARVTRYELADVRAIRENLNIT
                     QSEMAKVLGTSLDTIKSWETGRRNPTGLAAKVLATIQANPKFFDELAAH"
                     /name="nadS"
     gene            complement(1985..2317)
                     /locus_tag="HUZ87_RS22710"
                     /old_locus_tag="HUZ87_22535"
     CDS             complement(1985..2317)
                     /locus_tag="HUZ87_RS22710"
                     /old_locus_tag="HUZ87_22535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_032242302.1"
                     /translation="MSHAIEFIETTMFTRQIKQIATDDEIKELQKELIESPDKGDLIQK
                     TGGLRKIRMATGNQGKSGSARVIYFLATAEVIYLVMAYPKSTKDSITDAEKAELKKLTQ
                     RLKDEV"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2462..2698)
                     /locus_tag="HUZ87_RS22715"
                     /old_locus_tag="HUZ87_22540"
     CDS             complement(2462..2698)
                     /locus_tag="HUZ87_RS22715"
                     /old_locus_tag="HUZ87_22540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_032242304.1"
                     /translation="MTEMERQLLSALESLQTSYEQQKQAWQDSYSSLQRMFETTSQALE
                     RNDRVCLNLSNQVNALHEQVERLSSDLSRLTRS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            3043..3411
                     /locus_tag="HUZ87_RS22720"
                     /old_locus_tag="HUZ87_22545"
     CDS             3043..3411
                     /locus_tag="HUZ87_RS22720"
                     /old_locus_tag="HUZ87_22545"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032242306.1"
                     /translation="MFSYLKAMYHQSKIQAELKAQIHEQTTVNAICHHPESIEIIAVCS
                     TDAYYRKRKDAAFLTTCSVLMRTLKDESVPMVLRKTAWRLLNERYQRIKLNQAYRIENF
                     LLVADFEYALEEHDELAE"
                     /name="hypothetical protein"
     gene            complement(3841..3975)
                     /locus_tag="HUZ87_RS22725"
                     /old_locus_tag="HUZ87_22550"
     CDS             complement(3841..3975)
                     /locus_tag="HUZ87_RS22725"
                     /old_locus_tag="HUZ87_22550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072003336.1"
                     /translation="MHEPPVQSDRCVLSGNYRLESNPERHAKTPLAAAIGKQDVREMQ"
                     /name="replication initiation protein"
     gene            complement(5159..5476)
                     /locus_tag="HUZ87_RS22730"
                     /old_locus_tag="HUZ87_22555"
     CDS             complement(5159..5476)
                     /locus_tag="HUZ87_RS22730"
                     /old_locus_tag="HUZ87_22555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="WP_032242326.1"
                     /translation="MKKIFILSAALIMAGCATKQYPQAVNVTGPESSAMDCKAVALEIA
                     KTRSVQQEIEETGEFDGRTVLGFLGDFGIGNGMAKGEARKKAANRLNQLEALKAAKCAD
                     Q"
                     /name="lipoprotein"
     gene            6561..6710
                     /locus_tag="HUZ87_RS22735"
                     /old_locus_tag="HUZ87_22560"
     CDS             6561..6710
                     /locus_tag="HUZ87_RS22735"
                     /old_locus_tag="HUZ87_22560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_167776965.1"
                     /translation="MPSEHDEQLDSTDTQLPGEYDLFDLMAQVTEENLHALVDFGPPVG
                     REAL"
                     /name="hypothetical protein"
     gene            7170..8398
                     /locus_tag="HUZ87_RS22740"
                     /old_locus_tag="HUZ87_22565"
     CDS             join(7170..7485,7485..8398)
                     /locus_tag="HUZ87_RS22740"
                     /old_locus_tag="HUZ87_22565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="WP_088895425.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAASQ
                     LFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVEYG
                     RGKKVDSARALIARGWGVSLVSRCLRVSRAQLHVILRRTDDWMDGRRSRHTDDTDVLLR
                     IHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYRIMRQNALLLERKPAVPPSKR
                     AHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREALHWAVTTGGFNSETVQ
                     DVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRSPESNGI
                     AESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREYLRQRACN
                     GLSDNRCLEI"
                     /name="IS3-like element IS2 family transposase"
     gene            8429..8797
                     /locus_tag="HUZ87_RS22745"
                     /old_locus_tag="HUZ87_22570"
     CDS             8429..8797
                     /locus_tag="HUZ87_RS22745"
                     /old_locus_tag="HUZ87_22570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_020936656.1"
                     /translation="MANFNPGPRTAIAAVRDSKNDIETWKKNGIRREEIVTVLNERYGL
                     NISLVAFDKALYRLRKSTDEPSLADVDSVSAAETNFSEASGPDNGFFLSGNDFMRAEEE
                     SQRVVESFRDSLSRKKGF"
                     /name="hypothetical protein"
     gene            8882..9865
                     /locus_tag="HUZ87_RS22750"
                     /old_locus_tag="HUZ87_22575"
     CDS             8882..9865
                     /locus_tag="HUZ87_RS22750"
                     /old_locus_tag="HUZ87_22575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /GO_function="GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="WP_151166972.1"
                     /translation="MKKKFSIIVPVYNVEKYLRECIQSVLDQDFNDYEIICVNDGSTDG
                     SLDIILSFDDQRIKVINQENKGLSVARNVAIESSTGEYIWFLDSDDKISHGSLKLIDKI
                     TNEKNYGIIFFNTHVFFDTPELKKKGWHDYKRSTFGEMDALTFFEKEMTNKKLIVSACC
                     YVFRRELLNGNAFIPHIYHEDNHFIATLIFDNYSTICLSLDEALYLRRVRNDSIMTSKK
                     SMRHIIGYSTCIDELTKYKKTKGIQNKSFDRFVNGMVLSLFSIAETIKTKEAMNYVARY
                     KKHPDLSFKDRALINYPLIKKTYLKLKNGKVFNYLRNGIINKKSNP"
                     /name="glycosyltransferase family 2 protein"
     gene            complement(10324..11021)
                     /locus_tag="HUZ87_RS22755"
                     /old_locus_tag="HUZ87_22580"
     CDS             complement(join(10324..10772,10772..11021))
                     /locus_tag="HUZ87_RS22755"
                     /old_locus_tag="HUZ87_22580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="WP_223368647.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRASARIMGVGLNTVLRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEHWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1 family transposase"
     Typing          6353..6526
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.143"
                     /coverage="100.0"
                     /evalue="5.58e-78"
     Typing          complement(3868..4233)
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.185"
                     /coverage="89.0"
                     /evalue="2.91e-169"
     Typing          complement(5878..6386)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.51"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tttcttttaa tgtcgcattg ggtattttta gttatattgt tttttatacc ttgtttaatt
       61 tcaatgaggt tgttaggaca ggagtaattc ataagttaag atgcatgacc aagttaaagt
      121 gattcattta ttaaattcat taaatgaatt ggtaatgact ccaacttact gcttgtgttt
      181 tatgttcaga taatgcccga taccgacttc ggcctaattt ttttaagtgt cgtaaaatcg
      241 tgttgaggac agtgcccata atgcgggatg tggcccggca tccaacgcca ttcatggcca
      301 tatcaatgat tttctggtgt gtaccgggct cagaggcggt gtaagtgaac tgtagttgct
      361 tgctatgttt tacgacagtg agagcagaga tagcgttgat gtccggtagt gcttttaccg
      421 ttacgcacca cgccttcagt agctgaacag gagggataac tgatggaaat ggaagccacg
      481 ggagcacctc aaaaacacca ttagacacta aaccagcaag ttggcatcat cacaaacagt
      541 tgttactgct ggaaaaaaaa cactatcaac acttaagtaa tggtgattca ataattaaga
      601 ttcagtatgt aaaatactga atcttaatta taaattttcc tttttctaaa aaatgtagcg
      661 cgactaatac ctaaagcaac ccaaggctcc cctagagaca gcctacctcc tcctgatgat
      721 ttttcaccac caacttttcc tcttttggct tgtataagtg aaaaattttg cttattaaat
      781 ttcaaaaatg tccacttgca gatacttttc gctattcccg ttacttcatt ctcattcaaa
      841 ggcactgcaa attgcagatt gtaagcgcag gcgcgttcat ggcaggcttg tagccactgt
      901 tcgtactcag gccagccctg acggatagcg cgatatgccc actttctggt tttatcaaac
      961 agtatgcagt tacgacctaa gccataatcg gcgacgattt ttttatcgtt cgcagcgttc
     1021 aggtcgagtg attcggctaa ccagtccagc gtgtagagtt caggctgcca tacgggcaat
     1081 tttccagtga ctgtggttcg gattcttgca aattagcccc gaataccccg tgtcagcgcc
     1141 caatttttta cgtagcgcgc tctctacagc agcggcgtaa cgtaacggtt tcatttttcc
     1201 gtctggcgcg gtgcgtatcg aggttttcag tgcgtacagc aggtgcgcgt gtccgttttc
     1261 ggggttggtg atggtcagtg tcggcgcggg ggcgttgcgg tcgctccagt caatggctgc
     1321 gccgatgcgg tcaacgtcaa atcccagcca gaacatggcg tgaggctgat taaactggat
     1381 gtatttagcc agaatagcgc gctctttccc tgcgatgcgg acaccaaagt gtaaatcatc
     1441 cgagaagtac ggtttatgcg gcaagcggtc gttgaaaagt tcgagtgctg cgttacgcaa
     1501 tgtgccctga acttatggcg ggctgttgtc tggcacggtt catgtgctaa tatccttctt
     1561 aggttttata cctagccctg atttatcatg tctcttccgc caagttgaaa tcatgattat
     1621 cggggctttg ttttttctgt tgccgttaaa gttacacgcg aacggcctcg cctgtcgaat
     1681 ttcttctgtc acatcagctt aatgcgccgc cagttcatca aaaaactttg ggttagcctg
     1741 aatggtggcc agcacttttg ctgccagtcc ggttgggtta cggcgtccgg tttcccagct
     1801 tttgatggta tcgaggctgg ttcccagcac tttcgccatt tcgctctgag taatattcag
     1861 gttttctctg atagccctga cgtctgccag ctcgtagcgg gtcacacggg cgggggcttt
     1921 ggttccgttt ttgatttcaa cggcctcttc cagagaggct ttcagctcgt caaaaaaact
     1981 catcttacac ctcgtctttc agtcgttggg tcagcttctt cagttctgct ttctctgcgt
     2041 ctgtgatgct gtctttggtg cttttagggt aggccatcac cagataaatc acctctgcgg
     2101 tggcaaggaa gtaaatcacc cgcgcactgc cgctctttcc ctgattcccc gtcgccatcc
     2161 tgatctttcg cagaccgccc gttttctgga tgaggtcacc tttgtccggt gactcgataa
     2221 gctctttctg aagctctttg atctcatcat ccgtggcgat ttgctttatc tgacgggtaa
     2281 acatcgttgt ctcgataaac tcaatcgcgt gactcacggc tgtctcctgt atattttgat
     2341 gtacaatgta caccaagagg cgtgttctga aaagccgcgt tctgtgccat cagacgttac
     2401 atggactgac ctcgatagcg ggttgcttgc ttctcctgct gtaactgccg ttcctgctgc
     2461 tttacgagcg tgttaatcgg ctcagatcgc tgctcaagcg ttcgacctgt tcgtgcaagg
     2521 cgttcacctg attgctcaag ttcagacata ccctgtcgtt gcgctccagt gcctgtgagg
     2581 tagtctcgaa catgcgttgc aagctgctgt aactgtcctg ccacgcctgc ttctgctgtt
     2641 cgtagcttgt ttgtaagctt tctaaggcgc tcagaagctg cctttccatc tcggtcatgg
     2701 tttttcctgt cctcagtgat aaaaacgtgc tgtggcgttt ctgtggtgtc tgacggggtt
     2761 atttcgcggt gtacttcatc acggtgtttc cggctgtcga tgtgtatgtc tcggtgccat
     2821 gcgctttctt cggcagtacc accagatagg tgttcttctg cttgtcgtcc ttgtaaatcg
     2881 ctacccctgc gccgctctgg ggtccgcttg gaaaaaggaa attatcctac gctaagcgca
     2941 ttttttgcac agatggctac cgactgaacg taacgtgtga gtcatgatca gaaatgtgtt
     3001 aactctagga attgacacga cttactgttt gatggggaaa tcatgtttag ctatctcaag
     3061 gctatgtatc atcaatcaaa aattcaggct gaactcaagg cacagataca tgaacaaact
     3121 acggttaatg ctatctgtca tcaccctgaa tcaatcgaga tcattgcggt ttgttcgact
     3181 gatgcgtatt accgaaaacg taaggacgct gcttttctga caacctgttc tgtgctcatg
     3241 cgtactttaa aagatgaatc cgtaccgatg gtactacgga aaacagcatg gcgattattg
     3301 aacgaaagat accaacgaat taagttaaat caggcttata ggatagaaaa tttcttgctt
     3361 gttgccgatt ttgaatatgc tctcgaagaa catgatgaac tagcagagta gtactagtga
     3421 ggcgatgaat accgacagtt aacgtgcatg tgacttaaac gtccgattac gtacagcatg
     3481 tagacaacat gtccgaaagt aagcaaaacc attaacggac agccggatca tttcgtacat
     3541 cccttacggt caggggttcg cgctcaatgg aacgaaatca cccgttagct taaaacgctt
     3601 aacgtaggat aatttccttt ttccaagcgg acccgcaata cagcaaggaa cttcttgaga
     3661 tcctttctgt tgcgtcgtaa tctcttgctc tgtaaacgaa aaaaccaccc tggcaggtgg
     3721 tttttcgaag gttagctaat cctggcagat tttctaaccg cggtaacagt cttgtgcgag
     3781 acatgtcacc aaatctgtcc tttcagtgta gccgcactca ggccatcact tcaagaactc
     3841 ttactgcatc tctcgcacat cctgtttacc aatggccgct gccagtggcg tttttgcgtg
     3901 tctttccgga ttggactcaa gacgatagtt accggacaag acgcagcggt cggactgaac
     3961 ggggggttcg tgcatacagt ccagcttgga gcgaactgcc tacccggaac tgagtgtcag
     4021 gcgtggaatg agacaaacgc ggccataaca gcggaatgac accggtaaac cgaaaggcag
     4081 gaacaggaga gcgcacgagg gagctaccag ggggaaacgc ctgggatctt tatagtcctg
     4141 tcgggtttcg ccaccactga tttgagcgtc agattctgtg atgcttgtca ggggggcgga
     4201 gcctatggaa aaccggcagc gccgcggcct ttcctccctc gctttatctc gtcttcatcg
     4261 ccatatgcct ttgtgaatat tctccgtccg ctcgccgcag ccgaacgacc gagcgtagcg
     4321 agtcagtgag cgaggaagcg gaatatcttc ggcctcacgt ccggcacctt acactccgta
     4381 actgcatttt ttcataggat gcaggatagg gctaacgccg caaagtgacg tcacctgacg
     4441 ttcctggatt acaaaggtta aagcagcgga caggaaatgt tttgtgccca cagctatgct
     4501 gggttattca caaaacagcg ggacaaaatg tgtttttgga gtaccgccgg cccgctaaag
     4561 actcctggtt agctcccgca tgtttgctat ctttttgcta ccattttgct acttttttgc
     4621 tatctccaga gtcttctggt ctttcggttg ctatcctttt gctacttttt tgctatcaaa
     4681 atgctacctc tcccttcttg caataaatga ccaaggcacg ttagcgatgt atacatcgcc
     4741 gcgccgcaag ggtgcccctt ccgaatcccg ttcatcactg tgtgctgatt aatccaggtt
     4801 aaatccgacc cccttagcaa taaatcctgc catgcctgac ggcctggcca acgcctcacg
     4861 gtcgcacagt aaactgcgca ccctttcacc gttatcggtg gggttcgtgg tggctttcag
     4921 ggcgctcgcc ggattttagc cgctaaaatg acgtccatgc gttcgtgtgc atagccatgc
     4981 agacgcgaag aaatcatcag gcgcgttttt tcacgatatg gacagggaaa gatgccggaa
     5041 ataggcagga gggcgcagaa atgccctgag ggcgtctgag atggtgcacg caacggatgt
     5101 tatggtaaat tcagctgatt ggcaggtaca aaaaaagccc cgagggcttt tttgtacctt
     5161 attgatctgc acactttgca gccttcaggg cttccagctg atttagccgg ttagctgctt
     5221 ttttgcgggc ttcgcctttt gccatgccat ttcctatccc aaaatcaccc aggaaaccta
     5281 atacggtgcg cccgtcaaat tcgccggttt cctcaatttc ttgttgtaca ctgcgcgttt
     5341 tggcgatttc cagagctacc gctttgcaat ccattgcgga agattctgga ccggtgacgt
     5401 tcacggcctg tggatattgc tttgttgcgc atcctgccat gatcagcgct gccgatagga
     5461 tgaatatttt tttcaaaatt atccttattt atttaggcat agttaagaga tacccatttt
     5521 atcggcagga aatgagggtt ttttagttga attttgggag gggcaaggtt ccatattggt
     5581 cgaggattct ggagctgacc ggcaacggcc agcgcggcta tgggccatgc ggtggttgag
     5641 catagaaagg atcttcttga gttccttttt ttctacgcgt aatctcttgc tctgaaaacg
     5701 aaaaaaccac ctggggaggt ggtttttcga aggttaagtc agttggggaa ctgcttaacc
     5761 tggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     5821 taggccacca cttcaagaac tctatagcac cacctacatt aactcgctct gctaatcctg
     5881 ttaccagtgg ctgctgccag tggcgctttg tcgtgtcttt ccgggttgga ctcaagacga
     5941 tagttaccgg ataaggcgca gcagtcggac tgaacggggg gttcgtgcat acagtccagc
     6001 ttggagcgaa ctgccttccc ggaactgagt gtcaggcgtg gaatgagaca aacgcggcca
     6061 taacagcgga atgacaccgg taaaccgaaa ggcaggaaca ggagagcgca cgagggagcc
     6121 gccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc actgatttga
     6181 gcgtcagatt tcgtgatgct tgtcaggggg gcggagccta tggaaaaacg gctttgccgc
     6241 ggccctatcg cttccctgtt aagtatttcc ctggcatctt ccaggacatc tccgccccgt
     6301 tcgtaagcca tttccgctcg ccgcagtcga acgaccgagc gtagcgagtc agtgagcgag
     6361 gaagcggaat atatcctgta tcacatattc tgctgacgca ccggtgccgc cttttttgtc
     6421 ctgccacatg aagcacttca ctgactccct catcagtgcc aacatagtaa gccagtatac
     6481 actccgctag cgctacgtga ctggttcagg gctgcgcccc gaaaccctct aacaacctct
     6541 cagctactgg atatcacccc atgccatctg aacatgatga acaactggat tcgactgata
     6601 cgcagcttcc cggcgaatat gacctttttg atttaatggc gcaggttact gaagagaacc
     6661 tgcatgccct ggtggatttt gggccgcctg tcggccggga agcgttatag ctttttcgct
     6721 tacccgcgta cgtggtaata cgccggtatt acatcttatt tctttatttt catgtaatta
     6781 caggtggttg taatacgttg cgtattacat aaataaacgg ggacttcagt ttttatcttt
     6841 taattcatgc aattgcgatg aaaatcgcgg ttgcgtatgg tgtatgacca tttaagggat
     6901 aatgtaacct gacttttaaa gaatgaatct atctttggca gcgcatgaga tcgataaaaa
     6961 gcgttccaca tcaggcggtt catgtatcca tgaaccggaa aggacaaaaa taagacaatt
     7021 gtaagacatt ttttgcgttt tactcttatt ttggactatt atgagacaaa tgtaagacta
     7081 gactggcccc ctgaatctcc agacaaccag tatcacttaa ataagtgata gtcttaatac
     7141 tagtttttag actagtcatt ggagaacaga tgattgatgt cttagggccg gagaaacgca
     7201 gacggcgtac cacacaggaa aagatcgcaa ttgttcagca gagctttgaa ccggggatga
     7261 cggtctccct cgttgcccgg caacatggtg tagcagccag ccagttattt ctctggcgta
     7321 agcaatacca ggaaggaagt cttactgctg tcgccgccgg agaacaggtt gttcctgcct
     7381 ctgaacttgc tgccgccatg aagcagatta aagaactcca gcgcctgctc ggcaagaaaa
     7441 cgatggaaaa tgaactcctc aaagaagccg ttgaatatgg acgggcaaaa aagtggatag
     7501 cgcacgcgcc cttattgccc ggggatgggg agtaagctta gtcagccgtt gtctccgggt
     7561 gtcgcgtgcg cagttgcacg tcattctcag acgaaccgat gactggatgg atggccgccg
     7621 cagtcgtcac actgatgata cggatgtgct tctccgtata caccatgtta tcggagagct
     7681 gcccacgtat ggttatcgtc gggtatgggc gctgcttcgc agacaggcag aacttgatgg
     7741 tatgcctgcg atcaatgcca aacgtgttta ccggatcatg cgccagaatg cgctgttgct
     7801 tgagcgaaaa cctgctgtac cgccatcgaa acgggcacat acaggcagag tggccgtgaa
     7861 agaaagcaat cagcgatggt gctctgacgg gttcgagttc tgctgtgata acggagagag
     7921 actgcgtgtc acgttcgcgc tggactgctg tgatcgtgag gcactgcact gggcggtcac
     7981 taccggcggc ttcaacagtg aaacagtaca ggacgtcatg ctgggagcgg tggaacgccg
     8041 cttcggcaac gatcttccgt cgtctccagt ggagtggctg acggataatg gttcatgcta
     8101 ccgggctaat gaaacacgcc agttcgcccg gatgttggga cttgaaccga agaacacggc
     8161 ggtgcggagt ccggagagta acggaatagc agagagcttc gtgaaaacga taaagcgtga
     8221 ctacatcagt atcatgccca aaccagacgg gttaacggca gcaaagaacc ttgcagaggc
     8281 gttcgagcat tataacgaat ggcatccgca tagtgcgctg ggttatcgct cgccacggga
     8341 atatctgcgg cagcgggctt gtaatgggtt aagtgataac agatgtctgg aaatataggg
     8401 gcaaatccaa agacattatg ggcggtatat ggcgaatttt aaccctggtc caagaacagc
     8461 cattgcagct gtcagggatt ccaagaacga tatagaaacc tggaagaaaa atggtattcg
     8521 tagggaagag attgtgactg tgctgaatga acgttacggc ctgaatatct cactggttgc
     8581 gtttgataag gctctttaca gattgagaaa aagtacagat gaaccgtctc ttgctgatgt
     8641 ggattctgtg tctgctgcag aaacgaattt ctctgaagcc agtgggcctg acaatggttt
     8701 ttttctgtcc gggaatgact tcatgcgagc agaagaagaa tctcaaagag tggttgagtc
     8761 attcagagat agccttagca ggaagaaagg attctgattt attcaacaaa gccgttgata
     8821 aatcgcattc cataaataac aacttgatgt tagtataact aaatattgtt tggagattac
     8881 tgtgaaaaaa aaattttcga taatagtacc tgtatataac gttgaaaaat acttaaggga
     8941 atgtattcaa tcagtattag atcaggattt taacgactat gagataatct gtgtaaatga
     9001 tggttcaaca gatggcagtc tagatatcat actgtcattt gatgaccaaa gaatcaaagt
     9061 aataaatcag gaaaataaag gcctgtcggt ggcaagaaat gtcgccattg aatcatcaac
     9121 tggtgaatat atatggtttt tagatagtga tgataaaata tcacatggaa gtctaaagtt
     9181 aatagacaag ataaccaatg aaaaaaatta tgggattatt ttctttaata cacatgtttt
     9241 ttttgacact ccagaactga aaaaaaaagg ctggcatgat tataagcgtt caacatttgg
     9301 tgaaatggat gcattaactt tctttgaaaa agaaatgacg aacaaaaaac taatagtgtc
     9361 agcatgctgc tatgttttta gaagggaatt attaaatggc aatgcattca ttccgcatat
     9421 atatcatgag gataatcact ttatagcaac cctgattttt gacaattatt caactatatg
     9481 cctctcgttg gatgaggcgt tatatcttag gagggtaaga aatgattcca taatgacgtc
     9541 taaaaaatca atgaggcaca tcattggata ttcaacatgc atcgatgagc ttacgaagta
     9601 taaaaaaaca aaaggcatac aaaacaagag ctttgataga tttgtgaatg gaatggtttt
     9661 gtcgttattc tctatagcgg aaacaataaa aacaaaggaa gcaatgaact atgtggcgag
     9721 atataaaaaa caccccgacc tatcatttaa agatagagca ttaattaatt acccattaat
     9781 taaaaagact tatttaaaac taaaaaacgg gaaggtgttt aattacctaa ggaatggcat
     9841 tattaataaa aaaagcaacc cttaataaaa gcaaagcgat aattaataaa ggtttttctt
     9901 tgttgaataa ataagatttc ggataggtat ccccgtgacg ggttgggttt tcaggcgata
     9961 cgcacgcttt ccggggcttt gttgaataaa tcgaactttt gctgagttga aggatcagat
    10021 cggggtcagt ttggagatcg gaaatttttg tacgttaagc gcattattta accaatcttg
    10081 gttcgtaagg ggttgatttt gcgtcagtgg ctatcgcttt taagttgtcg ctcttgcagt
    10141 atcgcattta ggttatccga ttaaggtcaa acctctgaaa atgccgtata gcgcgggagt
    10201 acaccctatt gctataggta agtctgttca aaaaacaggc ttaccgtaca ataattctct
    10261 atatccaaac taaccccaga tcacgcatct tcccgacaac gcagaccggg taatgactcc
    10321 aacttactga tagtgtttta tgttcagata atgcccgatg accttgtcat gcagctccac
    10381 cgattttgag aacgacagtg acttccgtcc cagccttgcc agatgttgtc tcagattcag
    10441 attatgtcgc tcaatgcgct gcgtatatcg cttgctgata acgtgcagct ttcccttcag
    10501 gcgtgattca tacagcggcc agccatccgt catccatacc acgacctcaa aggccgacag
    10561 caggctcaga agacgctcca gtgtggccag agtgcgttca ccgaagacgt gcgccacaac
    10621 cgtcctccgt atcctgtcat acgcgtaaaa cagccagcgc tgacgtgatt tagcaccgac
    10681 gtagccccaa tgttcgtcca tttcagcgca gacaatcaca tcacttcccg gttgtatgcg
    10741 cgaggttacc gactgcggcc tgagtttttt aagtgacgta aaaccgtgtt gaggccaacg
    10801 cccataatgc gtgcactggc gcgacatccg acgccattca tggccatatc aatgattttc
    10861 tggtgcgtac cgggctgaga ggcggtgtaa gtgaactgta gttgccatgt tttacggcaa
    10921 tgagagcaga gatagcgctg atgtccggca gtgcttttgc cgttacgcac cacgccttca
    10981 gtagcggagc aggaaggaca tctgatggaa atggaagcca cgcaagcacc ttaaaatcac
    11041 catcatacac taaatcagta agttggcagc attacccact attggtggag atgatcataa
    11101 tgccttataa tcatacggga tacgaactta taaagtccta aagtttagct aaatcggaat
    11161 ctacaacgta gatgatagct tgaagaatta tgatgtcttt tatggtggta tctttaaaac
    11221 catcacggat tataaattat tgaaaattca aaaggatatt aaaaacaata tgactgcact
    11281 agtcagtagt tttaaatggg ttggcattgg acaacttgta aaaatagcaa cacaattggt
    11341 ttcgttattc tacttgacgc acttaatccc cccatcgagc tatggactat tagcaatgtc
    11401 atctgtggtt atgaatttag caagcatatt caatgacatg ggaactgggg cggcagtaat
    11461 tcagcgacaa gaggcgtcga tcggttttta taattacata tacaggataa acatttttac
    11521 tgggttgttt gttatgctgc ttgtaataat aacatcccca ttaattgtcg tgtactttaa
    11581 cacacccgag ctatacccgg ttctatttct attggctttt agctttccaa tttccagctt
    11641 aacaataact cacaaagcaa aattagaaaa agaaatgaac tttaaagtca tcattgcaat
    11701 tgaaattttc tctagcctta caggaatggt gctggcaata gttttagcta atttaggctt
    11761 tggtgtttat agtttgattt ttcaaacact gacaacaata tcaatatcca caatatgttt
    11821 tttgaaggtt tccgggttga gtttatcatt gagaattaaa aatatcagcc atagtgacaa
    11881 gtcaggagtt atagggttta gtggttattt attgtctttt aatttaataa attatttttc
    11941 caggaatctt gattctattt taataggccg ttttttctca tccgcaatac ttggggtcta
    12001 ttcgattgcc taccgcatta tgttatttcc gttgcagagc ctgacgcttg ttttttctag
    12061 agttttcttg ccacacctta gcaaaaacat aaataacact aataacacta ataaacaaga
    12121 ttacttaaat gcattaaaac ttatactatc attaagcgcg ccactgatgc taggtttatc
    12181 tgctacatct ttttctttta cgaggatttt ctttgatcct aagtggcacc tacttggaga
    12241 cttattgcat tggttggcac ctacagcgat tatccaatca gccttgagta caacaggttc
    12301 tgtttttatg gcctacggaa aaacaaggtg gttgtttttc ttaggttgca ttggcgcttt
    12361 tttgatgggg ttgtctttca ttataggtgt tcatttcgat ataaaaacat tggtctacct
    12421 ttattttttt gcaaacttaa ttaattcact cccggcattt attttactta agaatatctt
    12481 gaagtttagt tacattgaat tggtaaacgt gttttttaga actatctccc cagctttttt
    12541 gatgttctta cttattaaag tatggcagca tttttccttt ttcaaaaatg ccagcattga
    12601 tt
//
