LOCUS       NZ_CP055080             6989 bp    DNA     circular CON 01-MAY-2024
DEFINITION  Shigella flexneri strain SWHIN_116 plasmid unnamed5, complete
            sequence.
ACCESSION   NZ_CP055080
VERSION     NZ_CP055080.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13925605
            Assembly: GCF_022354205.1
KEYWORDS    RefSeq.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6989)
  AUTHORS   Che,Y., Xu,X., Yang,Y., Brinda,K., Hanage,W., Yang,C. and Zhang,T.
  TITLE     High-resolution genomic surveillance elucidates a multilayered
            hierarchical transfer of resistance between WWTP- and
            human/animal-associated bacteria
  JOURNAL   Microbiome 10 (1), 16 (2022)
   PUBMED   35078531
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6989)
  AUTHORS   Che,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2020) Civil Engineering, The University of Hong
            Kong, Pokfulam Road, Hong Kong 000000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: "Other" v. Unicycler
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology :: Illumina; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022354205.1-RS_2024_05_01
            Annotation Date                   :: 05/01/2024 05:17:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,956
            CDSs (total)                      :: 4,829
            Genes (coding)                    :: 4,546
            CDSs (with protein)               :: 4,546
            Genes (RNA)                       :: 127
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 93
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 283
            CDSs (without protein)            :: 283
            Pseudo Genes (ambiguous residues) :: 0 of 283
            Pseudo Genes (frameshifted)       :: 81 of 283
            Pseudo Genes (incomplete)         :: 200 of 283
            Pseudo Genes (internal stop)      :: 45 of 283
            Pseudo Genes (multiple problems)  :: 44 of 283
            Pseudo Genes (short protein)      :: 4 of 283
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP055080.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6989
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /strain="SWHIN_116"
                     /isolation_source="Shek Wu Hui WWTPs_116 WWTPs influent"
                     /db_xref="taxon:623"
                     /plasmid="unnamed5"
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.5 N 114.1 E"
                     /collection_date="2018-12"
     gene            complement(627..818)
                     /locus_tag="HUZ90_RS24455"
                     /old_locus_tag="HUZ90_24220"
     CDS             complement(627..818)
                     /locus_tag="HUZ90_RS24455"
                     /old_locus_tag="HUZ90_24220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001554612.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDNGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            886..1209
                     /locus_tag="HUZ90_RS24460"
                     /old_locus_tag="HUZ90_24225"
     CDS             886..1209
                     /locus_tag="HUZ90_RS24460"
                     /old_locus_tag="HUZ90_24225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001444404.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1418..>1852
                     /locus_tag="HUZ90_RS24465"
                     /old_locus_tag="HUZ90_24230"
                     /pseudo=""
     CDS             1418..>1852
                     /locus_tag="HUZ90_RS24465"
                     /old_locus_tag="HUZ90_24230"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1887..2447
                     /locus_tag="HUZ90_RS24470"
                     /old_locus_tag="HUZ90_24235"
     CDS             1887..2447
                     /locus_tag="HUZ90_RS24470"
                     /old_locus_tag="HUZ90_24235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_024194137.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSLTA
                     LCVTGGIFLASWGLIQWQESRIAANILAIRDQEDTLAKLREKTWGVTYHEGRNGKFLVL
                     PSGVKGENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            2454..2687
                     /locus_tag="HUZ90_RS24475"
                     /old_locus_tag="HUZ90_24240"
     CDS             2454..2687
                     /locus_tag="HUZ90_RS24475"
                     /old_locus_tag="HUZ90_24240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_001554706.1"
                     /translation="MTELEMHLLNALEQLQQDYMQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDHLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3171..3593)
                     /locus_tag="HUZ90_RS24480"
                     /old_locus_tag="HUZ90_24245"
     CDS             complement(3171..3593)
                     /locus_tag="HUZ90_RS24480"
                     /old_locus_tag="HUZ90_24245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001255518.1"
                     /translation="MRSAAARLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFYTQKADTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEKFFHSLRSTTA
                     FAGRKYTNSDMPSPKKMKLMADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            3715..3993
                     /locus_tag="HUZ90_RS24485"
                     /old_locus_tag="HUZ90_24250"
     CDS             3715..3993
                     /locus_tag="HUZ90_RS24485"
                     /old_locus_tag="HUZ90_24250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000710675.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
                     /name="hypothetical protein"
     gene            complement(3985..4275)
                     /locus_tag="HUZ90_RS24490"
                     /old_locus_tag="HUZ90_24255"
     CDS             complement(3985..4275)
                     /locus_tag="HUZ90_RS24490"
                     /old_locus_tag="HUZ90_24255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000047032.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
                     /name="hypothetical protein"
     gene            4516..6162
                     /gene="cka"
                     /locus_tag="HUZ90_RS24495"
                     /old_locus_tag="HUZ90_24260"
     CDS             4516..6162
                     /gene="cka"
                     /locus_tag="HUZ90_RS24495"
                     /old_locus_tag="HUZ90_24260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159420795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin K"
                     /protein_id="WP_159420795.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSGVHWGGGSGHG
                     NNGGQGNSNSSGSTSTVMKTGESYLTPWGDVVINNDGLPVMNGIVMTEENSTLVDNPFG
                     GVSRVLNSLISDMPSLFAESSGNNNNNTASVNTAPTNAQVSDMDKSSKVVSNVINEKQK
                     QKNKIATQISEKQKKIEEMKKVFKHHSYHGITDLERDVDELQKKSNQLDADISKLNSYK
                     NTLQSKIGDVNKQKEAEEKARENAEVAEHETLNEEKQAVAEAEKRLAEAKAELAKAESD
                     VQSKQATVSRVAGELENAQKSVDVKVTGFPGWRDVQKKLQRQLEAKQAEYSAVENELKN
                     AVSFRDGKAAEVKEAEQKLKEAQDALEKSQIKDAVDTMVGFYQYITEQYGEKYAKIAQD
                     LAEKSKGKKIQGVDEALAAFEKYKNVLDKKFSKVDRDAIFNALESVNYDELSKNLTKIS
                     KSLKITSRVSFLYDVGSDFKNAIETGNWRPLFVTLEKSAVDVGVAKIVALMFSFIVGVP
                     LGFWGIAIVTGIVSSYIGDDELSKLNELLGI"
                     /name="cka"
     gene            complement(6159..6449)
                     /gene="cki"
                     /locus_tag="HUZ90_RS24500"
                     /old_locus_tag="HUZ90_24265"
     CDS             complement(6159..6449)
                     /gene="cki"
                     /locus_tag="HUZ90_RS24500"
                     /old_locus_tag="HUZ90_24265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin K immunity protein"
                     /protein_id="WP_011264161.1"
                     /translation="MHLKYYLHNLPESLIPWILILIFNDNDNTPLLFIFISSIHVLLYP
                     YSKLTISRYIKENTKLKKEPWYLCKLSALFYLLMAIPVGLPSFIYYTLKRN"
                     /name="cki"
     gene            6479..6610
                     /locus_tag="HUZ90_RS24505"
                     /old_locus_tag="HUZ90_24270"
     CDS             6479..6610
                     /locus_tag="HUZ90_RS24505"
                     /old_locus_tag="HUZ90_24270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_077521118.1"
                     /translation="MKIIIFLITILFLSGCQANYIRDVQGGTVAPSSSSELTGIAVQ"
                     /name="colicin release lysis protein"
     gene            complement(6805..6948)
                     /locus_tag="HUZ90_RS24835"
     CDS             complement(6805..6948)
                     /locus_tag="HUZ90_RS24835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_152896351.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGNWIVDLDTQ
                     NS"
                     /name="replication initiation protein"
     Typing          455..542
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(2..444)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.258"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(6840..6989)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.013"
                     /coverage="100.0"
                     /evalue="5.27e-67"
     Typing          1200..2794
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.248"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 accaacagcg tgagctatga gaaagcgcca cgcttcccaa agggagaaag gcggacaggt
       61 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagccacca gggggaaacg
      121 cctggtatct ttaagtcctg tcgggtttcg ccacctctga cttgagcgtc ggtttttgtg
      181 atgctcgtca ggggggcgga gcctatggaa aaacgccagc tgcgtggcct tttccgggtt
      241 cttgtttttt gctcacatgt ttcttttggt gttatcccct gattctgtgg ataaccgcat
      301 taccgtcttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc ggagcgagtc
      361 agtgagcgag gaagcggaaa agcgcctgga cgagcatttt ctccttacgc atctgtgcgg
      421 catttcacac ccggcatggt gcacttttca tacaatcctc actgatgcca aatgattaag
      481 ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcta
      541 aaacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctca caggccagct
      601 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgttatcccc gaagcgtgcg
      661 aggcagctgc ggtaaagctc gtcagcgtga tcgtgaagcg cttcgcagat gtcagcctgg
      721 tcgtcggcgt ccagttcatt cagtttttcc agcagggtca gcgtctggct tctgatgaag
      781 cgggccatgt taagggcggt tttttcctgt ttagtcacag ttctgtttct ccatgtgtgg
      841 ggagtctgtc ggaggttttt aatgataccg gtgaaacgcg aaaggatgct cacgatacgg
      901 gtcacggatg aggaacatgc gcggctgctg gcacgttgtg agggaaaaca acttgcgtca
      961 tggatgcgta aggtctgcct gggggcaccg ccatcgaaaa cgtcaggtct tccgacactg
     1021 gcaccgccgc tgttgcgcca gttcgcttcg gtcggcaata acctgaatca gatagcccgg
     1081 aagataaaca gcggacagtg gtcggggcat gaccgggttc atgtggtggc tgcactgatg
     1141 gccatcggac gtgaactgag tgagctgagg gatgaggtcc gtaaacaggg agaacgggat
     1201 gatagttaag tttcatgcca ggggaaaagg cggtggcagt ggtccggttg attacctgct
     1261 gggccgggag cgtaaccggg aaggggcgag agttctgcgg ggtgctcccg aagaggtgcg
     1321 ggaactcatc gatgccacgc cgtttgcgaa aaaatacacc tcgggggttc tgtcttttgc
     1381 agagcagacc ctgccaccgg gagagcgtga aagggtgatg gagagctttg agcgggtact
     1441 gatgcccggt cttgaaaaaa atcagtacag catcctgtgg gtggagcacc aggacaaggg
     1501 gcgtcttgag ctgaattttg tcatcccgaa tatggagctg gcgagcggaa aacggctcca
     1561 gccctattac gaccgagcgg accgtccgcg aatcaatgcc tggcagacac tggtgaatca
     1621 ccattacggg ctgcacgacc cgaacgcacc ggagaacagg cggacacttg tcaccccgaa
     1681 taatctgccg aaagcgaaac aggaggccgc agaagcgatt acgaggggac tggaggctct
     1741 ttaccatgcc ggagcgctaa aaacgcgtca ggatgtcact gaggcgctta cagcggcagg
     1801 ttttgaggtt gtgaggacaa ccaggagcag tatcagtatc gccgacccgg agggtgggcg
     1861 taatctcagg ctgaaagggg cgctgtatga gcaatctttt acaaatgggg acagacttcg
     1921 agaagaaact gaaagagcgt gccgtctcta cagagaacat gctgaacagc gagttcagga
     1981 agctcgaagc gtctgtagac agggaactga acttaaacgg gcagaaaatc agagacgcta
     2041 tcagcgcaca cacagcagcg gtgaaagaac aactggagac gctgaacagt gccgtgaacg
     2101 cgcagttcag cgccacagag aacgaactgg cccggcggca ggaagagctg ctctggaagc
     2161 tggtcaaagg gcggatactg tacccgtcgc tgacggcgct ttgcgtcacg ggcgggattt
     2221 ttctggcgtc atgggggctg attcagtggc aggaaagccg gattgcggcg aacatcctgg
     2281 ctatcaggga ccaggaagat acgctggcga aactccggga aaaaacatgg ggtgtgacgt
     2341 atcacgaggg caggaacggg aaatttctag tgttgccgtc cggggtgaag ggggaaaaca
     2401 actggacggt ggagaagaag aacgcagtaa gactggtcag ggagtaagtg agtatgacag
     2461 agctggaaat gcacttactg aacgccttag agcaattgca gcaggactat atgcagcggc
     2521 tgaaagactg ggagagcgcc ttcacggatt tgcagcatat gttcgagctg acacgacagg
     2581 agaacgggca actgagggaa cagtgcgacg acttgagtca gcaggtgcag agattagccg
     2641 ggcaggtgga ccacttgagt cggttattta tcacgaacag cagataaggg atgaacagga
     2701 aaggcaggca cgcctggaac gggagcgtgc gcggtcctgg catgaagagc aggaacggga
     2761 agcacgggta tggcgtggcc cgtcaatagg tatgtgacgg tgagttgttc cgggaggttg
     2821 ccagatggta ccggaacgcg ttaaaaacgc atccgggcgc gattctggcg tgtcgttttt
     2881 gttgctgtgt tttgcctctt tgccagatcg cgctcattcc tcagcctcat cccgcgatgt
     2941 ctgcgtatgt catcaactga aaagatggaa aaaaaacagt gtcatgaggt ttacgcaggc
     3001 aaaaccagcg ttattcacat ggctgaatag ctatgcatac ggttaaaatt tatcaggtgc
     3061 gatcgcggca gcttttcggg tggtttgttg ccatttttac ctgtcagcag ccgtgatcgc
     3121 gctgaacgca tttcagcggt gcgtacaatt aagggattat ggtaaatccc ttactgtctg
     3181 ccctcgtatc catcgagata aaccgcagaa atggtgtctg ccattagttt catttttttc
     3241 ggcgaaggca tatcgctgtt tgtgtatttt ctgccagcaa aggctgttgt gctccggagt
     3301 gagtgaaaaa atttttctga gtggaattct tttacacgtt gctgtgccgt ttctgctgat
     3361 actccggctg ctttgtcttt catccccata tcccggaact gctgaaagaa cgggagcatc
     3421 atacgtatgg tgtctgcttt ctgggtgtag aagttaggat caaatccatc atctgaagca
     3481 taaacataat gccgggtatg tcgttccagg ctggtctttg gtgtgcatcc tgtaagagct
     3541 attgttgctg ttatcagagg tatcagaagc agcctggcag cggcagatct cataaacatc
     3601 tccttgcgtt tttaatcttg atactcaggg agttatatct ccgaagagta tctcttatca
     3661 tacgtcggtt tactcccaat gtgatttgat ggtcaatgag tcaggaataa tcccgtgaaa
     3721 aaatgtgtgc cagtcctgct ttttagtgtt ctttctgctt cggttgccag tcatgcggac
     3781 agcaatgttt ttcaacaaaa ggccgagaag acaaaaaacg gcattgttgc ggatgttaat
     3841 gccagacaga aaatgttcag taatagactg aagatacaac ttcagatcga tgagttgaga
     3901 gcagagctgg ctggcaccag agatgcccaa aaaagagagg atttgcgtaa aaaactggag
     3961 tctctggagc ggcaaaagaa atccttacgc tgatgggggc gtattttccc cggtttcttc
     4021 cataaccttt ttaccgttca gatatttacg ggcgaggcga atgcgtcttg cctggtatac
     4081 cgccgagtaa gcctgggtgg gattcacacc attactgatt tcactgacca catgacgctg
     4141 atcagccagt gtcagcgtgt aaacctgttt ctggatgagg tcacgggctt tgtcatgctc
     4201 atatccggca gcaacaaggg tgtcgagaag tcgtttgtct gttttgttca gtttatggcg
     4261 gggtgatttg ctcacatcgt tctccgtttt ttcatgatcc aacagtttta tatctcatcc
     4321 tgatttgctc cggtgttctg tttcgacgtt ctcaatgcct gcctccccat agtgtgatcc
     4381 ttattgcaaa aatgaaaatc catgctcttg acatggacaa tgctgagtag taggttttta
     4441 ctgtatataa aaccagtggt tatatgtaca gtatattgtt tttaatttat tgttttaatt
     4501 atcaaagagg aatttatggc taaagaacta agtggatatg gaccaactgc tggtgagtct
     4561 atggggggaa caggggcgaa tctgaatcag caaggtggaa ataataatag taatagtggg
     4621 gttcactggg gtggtggctc tgggcatggt aataatggtg gtcagggaaa ttcaaattca
     4681 tctggttcta catcaactgt gatgaaaacc ggtgagtcat atctgactcc gtggggtgat
     4741 gttgtcatta ataatgatgg ccttccggtg atgaatggta ttgttatgac agaagagaat
     4801 tcaactctgg ttgataatcc atttggaggg gtctccagag ttcttaattc tttaattagt
     4861 gatatgccat cactttttgc agaaagctca ggaaataaca acaataacac tgcttcagtt
     4921 aatactgcac caacgaatgc tcaggtcagt gatatggata agagttcgaa ggtggtgagt
     4981 aatgtaatta atgaaaaaca aaagcagaag aataaaattg ctacacagat tagtgaaaaa
     5041 cagaagaaaa tcgaggagat gaaaaaagta ttcaagcatc attcttatca tggtatcact
     5101 gatcttgaga gggatgttga tgagttgcaa aaaaaatcaa atcagcttga tgcagatatt
     5161 agcaaactta attcttataa aaatacattg caaagtaaaa ttggtgatgt gaataaacag
     5221 aaggaagctg aagaaaaggc tcgtgaaaat gcagaggtcg ctgaacatga aactcttaat
     5281 gaagagaaac aggcggtggc ggaagcagag aagcgtttag ctgaggcgaa agctgaactg
     5341 gcgaaggctg aaagtgatgt acagagtaag caagctactg tttccagagt tgcaggagag
     5401 cttgaaaacg ctcagaagag tgttgacgtg aaggtcaccg gatttccggg atggcgtgac
     5461 gttcagaaaa aactacagcg tcagcttgaa gctaagcagg ctgagtactc cgcagttgaa
     5521 aatgaactta agaatgctgt tagtttcagg gatggtaagg ccgctgaggt taaagaggca
     5581 gagcagaaat taaaagaagc tcaggatgct cttgaaaaga gccagataaa agatgctgtt
     5641 gatactatgg ttggatttta tcaatatata accgaacaat atggagaaaa atacgcaaaa
     5701 attgctcagg atttagcgga aaagtctaaa ggaaagaaaa ttcaaggtgt tgatgaagct
     5761 ctggcagcat ttgaaaagta taagaatgta ctggataaga aattcagtaa ggttgatcgg
     5821 gatgctattt ttaatgcgtt agagtctgtt aattatgatg agttatctaa gaatttaacg
     5881 aagatatcaa agtcgcttaa aatcaccagt cgagtatctt ttctatatga tgttggttct
     5941 gattttaaaa atgcaataga aactggtaac tggcgacctt tgtttgtcac gctggagaaa
     6001 agtgcggtcg atgtaggtgt ggcaaaaatt gtggcattaa tgtttagttt tattgttggt
     6061 gtgcctcttg gtttctgggg gattgcaatt gtcacaggta ttgtttcttc ttacataggg
     6121 gatgatgagt tgagcaaact taatgagttg ttagggattt aatttctctt tagagtgtaa
     6181 tatatgaaac ttggcaatcc tactgggatt gccattaata aataaaacaa tgcagataac
     6241 ttgcataagt accagggttc tttttttaac tttgtatttt ctttgatata tctagatatg
     6301 gttaatttag agtatggata tagcaataca tgtattgatg atataaatat aaataacaaa
     6361 ggagtgttat cattgtcgtt aaatattaaa ataagaatcc atggtataag tgattcaggt
     6421 aaattatgta ggtagtattt taaatgcata agtattttta cttttgttgg aaagaaatat
     6481 gaaaataata atttttctaa ttacaatatt attcttgtca ggatgtcaag caaactatat
     6541 tcgtgatgta caaggaggaa ccgttgcacc atcttcgtct tctgaactga cggggatcgc
     6601 ggttcagtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     6661 tgcctgtaaa cgaaaaaacc gcctggggag gcggtttagt cgaaggttaa gtcagttggg
     6721 gactgcttaa ccgggtaact ggctttagtg gagcacagat accaaatgct gtccttccag
     6781 tgtagccgct ataaggcttc aacttcaaga gttctgcgta tctaaatcca ctatccagtt
     6841 accaatggct gctgccagtg gcgttaagtc gtgtcttacc gggttggact caagacgata
     6901 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     6961 ggagcgaacg acctacaccg aactgagat
//
