LOCUS       NZ_CP055060             2107 bp    DNA     circular CON 01-MAY-2024
DEFINITION  Klebsiella pneumoniae strain SWHE4 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP055060
VERSION     NZ_CP055060.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13925582
            Assembly: GCF_022354105.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Che,Y., Xu,X., Yang,Y., Brinda,K., Hanage,W., Yang,C. and Zhang,T.
  TITLE     High-resolution genomic surveillance elucidates a multilayered
            hierarchical transfer of resistance between WWTP- and
            human/animal-associated bacteria
  JOURNAL   Microbiome 10 (1), 16 (2022)
   PUBMED   35078531
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Che,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2020) Civil Engineering, The University of Hong
            Kong, Pokfulam Road, Hong Kong 000000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: "Other" v. Unicycler
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology :: Illumina; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022354105.1-RS_2024_05_01
            Annotation Date                   :: 05/01/2024 05:24:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,667
            CDSs (total)                      :: 5,543
            Genes (coding)                    :: 5,401
            CDSs (with protein)               :: 5,401
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 49 of 142
            Pseudo Genes (incomplete)         :: 86 of 142
            Pseudo Genes (internal stop)      :: 23 of 142
            Pseudo Genes (multiple problems)  :: 15 of 142
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP055060.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="SWHE4"
                     /isolation_source="Shek Wu Hui WWTPs_4 WWTPs effluent"
                     /db_xref="taxon:573"
                     /plasmid="unnamed3"
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.5 N 114.1 E"
                     /collection_date="2018-12"
     gene            996..1187
                     /locus_tag="HUZ67_RS28245"
                     /old_locus_tag="HUZ67_28160"
     CDS             996..1187
                     /locus_tag="HUZ67_RS28245"
                     /old_locus_tag="HUZ67_28160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041614.1"
                     /translation="MNKQQKTAVNMAKFIQDQSLLLLDRLNELDLDHEADFCEKLHEDA
                     ERLYRSLSARLNEQQDGA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1955..2071)
                     /locus_tag="HUZ67_RS28250"
                     /old_locus_tag="HUZ67_28165"
     CDS             complement(1955..2071)
                     /locus_tag="HUZ67_RS28250"
                     /old_locus_tag="HUZ67_28165"
                     /inference="COORDINATES: protein motif:HMM:NF033853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein"
                     /protein_id="WP_142293809.1"
                     /translation="MDWISLAIAIALLVVAVYSGISYFREKKAISRVFRSKR"
                     /name="small membrane protein"
     Typing          complement(502..1121)
                     /name="rep_cluster_2327"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.013"
                     /coverage="99.0"
                     /evalue="2.5900000000000003e-161"
ORIGIN
        1 gatttcataa agataatatc atcttttttt tagcatgata ctgtcgttac ttattgtggt
       61 gagatgaaac atagccactg tttttgtatg tgtttgttaa ggccgttaag attattctta
      121 atcactgatt ttgtacatat catcaaaata aaaataaaaa aaactaatgc ccccgtggtg
      181 ggtatataac aaatcacggg gtgcaggtca gaactacccc aaaactttga atgatcgggt
      241 ggcagactaa atgaaatctt gagattcatt cagcctcgtc gtaatctctt tctctgtaaa
      301 cgaaaaaacc gccctgcaag gcggtttttc gaaggttcag taagttgggg aacttctgaa
      361 ccgtggtaac agtcttatgc gagacgtgtc acccaagctg tccttctagt gtagccgtag
      421 taagggcacc acttcaagaa ctctcacaat atctctcgca catcctgtta ccagtggctg
      481 ctgccagtgg cgcatttgca tgtctttccg ggttggactc aagacgatag ttaccggata
      541 aggcgcagcg gtcggactga acggggggtt cgtgcataca gtccagcttg gagcgaactg
      601 cctacccgga actgagtgtc aggcgtggaa tgagacaaac gcggccataa cagcggaatg
      661 acaccggtaa accgaaaggc aggaacagga gagcgcacga gggagctacc agggggaaac
      721 gcctgggatc tttatagtcc tgtcgggttt cgccgccact gatttgagcg tcagattttg
      781 tgatgcttgt caggggggcg gagcctatgg aaaaacgccc cggcggggcg tggccttttc
      841 gcccttgtcc ttagctttcg ttatgcttac cgttacgtta tctgcttatc ctccctccgc
      901 tcgccgcaga ctcatgaccg aacggagtga gcgaatgagc gaggaagcgg aaaaccatca
      961 gaacgtacag taagcacttt cgcgccgggg ccttcatgaa caaacaacaa aagactgccg
     1021 ttaacatggc taaattcatc caggatcagt ccctgcttct tctggacaga cttaatgagc
     1081 tggatctgga tcatgaggct gatttttgcg agaaactcca tgaggatgcg gaacgccttt
     1141 accgaagcct gtcagcgcgc ctgaatgagc agcaggacgg tgcgtaatgt ctccgttctg
     1201 agtattaccg gagtaatacc ggcatcgcct taattttcat agtgttagcg cttaacatta
     1261 ttaccccggt aatacccaga atccctgtat tttttctttt ttatttattt ttcatgaagt
     1321 tgcgatgaaa atcgcagctg cgttaggtgt atgaccattt tgaggttgaa aaataatcaa
     1381 ccatggcggg tgatttacgc aggttaaggg gagatcgtga caaaaaaaag cgttcctcac
     1441 tgtatcgttc atgcctgcat gaacgggtta ttcttcctga ctacagaatt acatcgctcc
     1501 ttcaggtcat acatcacttt tacaacactt ttacatcact ccttcgctct gtacatcact
     1561 tttacaacac ttttgcatca cttttacatc attccttcag ctcatacgtc acttccatcc
     1621 tctgtgccat ttgtacatcc ccccccttcc tgaacgtgct gaaaacggtt tttccggctg
     1681 cgccggcaac gcctcacggc cacgcaataa attgcgtccc cgttcaccgt tttcggatga
     1741 gaagaacgtc gaaaacagga cttccactgg ctgcacggtg tgaaattctg cgctatgcac
     1801 ttgcgcgcat actcatgcat gccgtaaaaa cagagcctgc gcgtttctgg cgggttttcg
     1861 ggtggtttgt tgcctgtttt accggtttcc cgtcagaaac gccctgaggc cgttttcgcg
     1921 gtgcgcgtaa gaagggatta tggtaaatag ggcgctatcg cttactccta aatacacgac
     1981 tgattgcttt tttttctctg aagtatgaga ttcctgagta gacagctaca acaagtagag
     2041 caatggcgat agcaagtgaa atccagtcca taaatgcctg attaatttaa taaaaacagc
     2101 ctgttat
//
