LOCUS       NZ_CP055018             6988 bp    DNA     circular CON 01-MAY-2024
DEFINITION  Shigella flexneri strain SWHEFF_71 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP055018
VERSION     NZ_CP055018.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13925593
            Assembly: GCF_022353885.1
KEYWORDS    RefSeq.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6988)
  AUTHORS   Che,Y., Xu,X., Yang,Y., Brinda,K., Hanage,W., Yang,C. and Zhang,T.
  TITLE     High-resolution genomic surveillance elucidates a multilayered
            hierarchical transfer of resistance between WWTP- and
            human/animal-associated bacteria
  JOURNAL   Microbiome 10 (1), 16 (2022)
   PUBMED   35078531
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6988)
  AUTHORS   Che,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2020) Civil Engineering, The University of Hong
            Kong, Pokfulam Road, Hong Kong 000000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: "Other" v. Unicycler
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology :: Illumina; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022353885.1-RS_2024_05_01
            Annotation Date                   :: 05/01/2024 05:16:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,174
            CDSs (total)                      :: 5,050
            Genes (coding)                    :: 4,786
            CDSs (with protein)               :: 4,786
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 93
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 264
            CDSs (without protein)            :: 264
            Pseudo Genes (ambiguous residues) :: 0 of 264
            Pseudo Genes (frameshifted)       :: 75 of 264
            Pseudo Genes (incomplete)         :: 180 of 264
            Pseudo Genes (internal stop)      :: 51 of 264
            Pseudo Genes (multiple problems)  :: 40 of 264
            Pseudo Genes (short protein)      :: 4 of 264
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP055018.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6988
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /strain="SWHEFF_71"
                     /isolation_source="Shek Wu Hui WWTPs_71 WWTPs effluent"
                     /db_xref="taxon:623"
                     /plasmid="unnamed3"
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.5 N 114.1 E"
                     /collection_date="2018-12"
     gene            complement(join(6878..6988,1..450))
                     /locus_tag="HUZ78_RS25685"
                     /old_locus_tag="HUZ78_25485"
     CDS             complement(join(6878..6988,1..450))
                     /locus_tag="HUZ78_RS25685"
                     /old_locus_tag="HUZ78_25485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067492.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSLTA
                     LCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVL
                     PSGVKGENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            complement(<485..1165)
                     /locus_tag="HUZ78_RS25935"
                     /old_locus_tag="HUZ78_25490"
                     /pseudo=""
     CDS             complement(<485..1165)
                     /locus_tag="HUZ78_RS25935"
                     /old_locus_tag="HUZ78_25490"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1128..1451)
                     /locus_tag="HUZ78_RS25695"
                     /old_locus_tag="HUZ78_25495"
     CDS             complement(1128..1451)
                     /locus_tag="HUZ78_RS25695"
                     /old_locus_tag="HUZ78_25495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001444404.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1519..1710
                     /locus_tag="HUZ78_RS25700"
                     /old_locus_tag="HUZ78_25500"
     CDS             1519..1710
                     /locus_tag="HUZ78_RS25700"
                     /old_locus_tag="HUZ78_25500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165980.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2378..2521
                     /locus_tag="HUZ78_RS25940"
     CDS             2378..2521
                     /locus_tag="HUZ78_RS25940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_152896351.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGNWIVDLDTQ
                     NS"
                     /name="replication initiation protein"
     gene            complement(2716..2847)
                     /locus_tag="HUZ78_RS25705"
                     /old_locus_tag="HUZ78_25505"
     CDS             complement(2716..2847)
                     /locus_tag="HUZ78_RS25705"
                     /old_locus_tag="HUZ78_25505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_077521118.1"
                     /translation="MKIIIFLITILFLSGCQANYIRDVQGGTVAPSSSSELTGIAVQ"
                     /name="colicin release lysis protein"
     gene            2877..3167
                     /gene="cki"
                     /locus_tag="HUZ78_RS25710"
                     /old_locus_tag="HUZ78_25510"
     CDS             2877..3167
                     /gene="cki"
                     /locus_tag="HUZ78_RS25710"
                     /old_locus_tag="HUZ78_25510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin K immunity protein"
                     /protein_id="WP_011264161.1"
                     /translation="MHLKYYLHNLPESLIPWILILIFNDNDNTPLLFIFISSIHVLLYP
                     YSKLTISRYIKENTKLKKEPWYLCKLSALFYLLMAIPVGLPSFIYYTLKRN"
                     /name="cki"
     gene            complement(3164..4810)
                     /gene="cka"
                     /locus_tag="HUZ78_RS25715"
                     /old_locus_tag="HUZ78_25515"
     CDS             complement(3164..4810)
                     /gene="cka"
                     /locus_tag="HUZ78_RS25715"
                     /old_locus_tag="HUZ78_25515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159420795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin K"
                     /protein_id="WP_162542215.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSGVHWGGGSGHG
                     NNGGQGNSNSSGSTSTVMKTGESYLTPWGDVVINNDGLPVMNGIVMTEENSTLVDNPFG
                     GVSRVLNSLISDMPSLFAESSGNNNNNTASVNTAPTNAQVSDMDKSSKVVGNVINEKQK
                     QKNKIATQISEKQKKIEEMKKVFKHHSYHGITDLERDVDELQKKSNQLDADISKLNSYK
                     NTLQSKIGDVNKQKEAEEKARENAEVAEHETLNEEKQAVAEAEKRLAEVKAELAKAESD
                     VQSKQATVSRVAGELENAQKSVDVKVTGFPGWRDVQKKLQRQLEAKQAEYSAVENELKN
                     AVSFRDGKAAEVKEAEQKLKEAQDALEKSQIKDAVDTMVGFYQYITEQYGEKYAKIAQD
                     LAEKSKGKKIQGVDEALAAFEKYKNVLDKKFSKVDRDAIFNALESVNYDELSKNLTKIS
                     KSLKITSRVSFLYDVGSDFKNAIETGNWRPLFVTLEKSAVDVGVAKIVALMFSFIVGVP
                     LGFWGIAIVTGIVSSYIGDDELSKLNELLGI"
                     /name="cka"
     gene            5050..5340
                     /locus_tag="HUZ78_RS25720"
                     /old_locus_tag="HUZ78_25520"
     CDS             5050..5340
                     /locus_tag="HUZ78_RS25720"
                     /old_locus_tag="HUZ78_25520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000047032.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
                     /name="hypothetical protein"
     gene            complement(5332..5610)
                     /locus_tag="HUZ78_RS25725"
                     /old_locus_tag="HUZ78_25525"
     CDS             complement(5332..5610)
                     /locus_tag="HUZ78_RS25725"
                     /old_locus_tag="HUZ78_25525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021565938.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KIFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
                     /name="hypothetical protein"
     gene            5732..6154
                     /locus_tag="HUZ78_RS25730"
                     /old_locus_tag="HUZ78_25530"
     CDS             5732..6154
                     /locus_tag="HUZ78_RS25730"
                     /old_locus_tag="HUZ78_25530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001255518.1"
                     /translation="MRSAAARLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFYTQKADTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEKFFHSLRSTTA
                     FAGRKYTNSDMPSPKKMKLMADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(6638..6871)
                     /locus_tag="HUZ78_RS25735"
                     /old_locus_tag="HUZ78_25535"
     CDS             complement(6638..6871)
                     /locus_tag="HUZ78_RS25735"
                     /old_locus_tag="HUZ78_25535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137427.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDHLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          complement(1796..1883)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          1894..2450
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.633"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3..1138)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.736"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(1511..1713)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="89.706"
                     /coverage="100.0"
                     /evalue="3.86e-12"
     Typing          complement(6532..6986)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.342"
                     /coverage="99.0"
                     /evalue="1.88e-60"
ORIGIN
        1 cacaccccat gttttttccc ggagtttcgc cagcgtctct tcctggtccc tgatagccag
       61 gatgttcgcc gcaatccggc tttcctgcca ctgaatcagc ccccatgacg ccagaaaaat
      121 cccgcccgtg acgcaaagcg ccgtcagcga cgggtacagt atccgccctt tgaccagctt
      181 ccagagcagc tcttcctgcc gccgggccag ttcgttctct gtggcgctga actgcgcgtt
      241 cacggcactg ttcagcgtct ccagttgttc tttcaccgct gctgtgtgtg cgctgatagc
      301 gtctctgatt ttctgcccgt ttaagttcag ttccctgtct acagacgctt cgagcttcct
      361 gaactcgctg ttcagcatgt tctctgtaga gacggcacgc tctttcagtt tcttctcgaa
      421 gtctgtcccc atttgtaaaa gattgctcat acagcgcccc tttcagcctg agattacgcc
      481 caccctccgg gtcggcgata ctgatactgc tcctggttgt cctcacaacc tcaaaacctg
      541 ccgctgtaag cgcctcagtg acatcctgac gcgtttttag cgctccggca tggtaaagag
      601 cctccagtcc cctcgtaatc gcttctgcgg cctcctgttt cgctttcggc agattattcg
      661 gggtgacaag tgtccgcctg ttctccggtg cgttcgggtc gtgcagcccg taatggtgat
      721 tcaccagtgt ctgccaggca ttgattcgcg gacggtccgc tcggtcgtaa tagggctgga
      781 gccgttttcc gctcgccagc tccatattcg ggatgacaaa attcagctca agacgcccct
      841 tgtcctggtg ctccacccac aggatgctgt actgattttt ttcaagaccg ggcatcagta
      901 cccgctcaaa gctctccatc accctttcac gctctcccgg tggcagggtc tgctctgcaa
      961 aagacagaac ccccgaggtg tattttttcg caaacggcgt ggcatcgatg agttcccgca
     1021 cctcttcggg agcaccccgc agaactctcg ccccttcccg gttacgctcc cggcccagca
     1081 ggtaatcaac cggaccactg ccaccgcctt ttcccctggc atgaaactta actatcatcc
     1141 cgttctccct gtttacggac ctcatccctc agctcactca gttcacgtcc gatggccatc
     1201 agtgcagcca ccacatgaac ccggtcatgc cccgaccact gtccgctgtt tatcttccgg
     1261 gctatctgat tcaggttatt gccgaccgaa gcgaactggc gcaacagcgg cggtgccagt
     1321 gtcggaagac ctgacgtttt cgatggcggt gcccccaggc agaccttacg catccatgac
     1381 gcaagttgtt ttccctcaca acgtgccagc agccgcgcat gttcctcatc cgtgacccgt
     1441 atcgtgagca tcctttcgcg tttcaccggt atcattaaaa acctccgaca gactccccac
     1501 acatggagaa acagaactgt gactaaacag gaaaaaaccg cccttaacat ggcccgcttc
     1561 atcagaagcc agacgctgac cctgctggaa aaactgaatg aactggacgc cgacgaccag
     1621 gctgacatct gcgaagcgct tcacgaccac gctgacgagc tttaccgcag ctgcctcgca
     1681 cgcttcgggg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     1741 ggcctgtgag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggttttagc
     1801 gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta
     1861 atcatttggc atcagtgagg attgtatgaa aagtgcacca tgccgggtgt gaaatgccgc
     1921 acagatgcgt aaggagaaaa tgctcgtcca ggcgcttttc cgcttcctcg ctcactgact
     1981 cgctccgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag acggtaatgc
     2041 ggttatccac agaatcaggg gataacacca aaagaaacat gtgagcaaaa aacaagaacc
     2101 cggaaaaggc cacgcagctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     2161 aaaaccgacg ctcaagtcag aggtggcgaa acccgacagg acttaaagat accaggcgtt
     2221 tccccctggt ggctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     2281 gtccgccttt ctccctttgg gaagcgtggc gctttctcat agctcacgct gttggtatct
     2341 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     2401 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     2461 aacgccactg gcagcagcca ttggtaactg gatagtggat ttagatacgc agaactcttg
     2521 aagttgaagc cttatagcgg ctacactgga aggacagcat ttggtatctg tgctccacta
     2581 aagccagtta cccggttaag cagtccccaa ctgacttaac cttcgactaa accgcctccc
     2641 caggcggttt tttcgtttac aggcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     2701 atcctttgat cttttctact gaaccgcgat ccccgtcagt tcagaagacg aagatggtgc
     2761 aacggttcct ccttgtacat cacgaatata gtttgcttga catcctgaca agaataatat
     2821 tgtaattaga aaaattatta ttttcatatt tctttccaac aaaagtaaaa atacttatgc
     2881 atttaaaata ctacctacat aatttacctg aatcacttat accatggatt cttattttaa
     2941 tatttaacga caatgataac actcctttgt tatttatatt tatatcatca atacatgtat
     3001 tgctatatcc atactctaaa ttaaccatat ctagatatat caaagaaaat acaaagttaa
     3061 aaaaagaacc ctggtactta tgcaagttat ctgcattgtt ttatttatta atggcaatcc
     3121 cagtaggatt gccaagtttc atatattaca ctctaaagag aaattaaatc cctaacaact
     3181 cattaagttt gctcaactca tcatccccta tgtaagaaga aacaatacct gtgacaattg
     3241 caatccccca gaaaccaaga ggcacaccaa caataaaact aaacattaat gccacaattt
     3301 ttgccacacc tacatcgacc gcacttttct ccagcgtgac aaacaaaggt cgccagttac
     3361 cagtttctat tgcattttta aaatcagaac caacatcata tagaaaagat actcgactgg
     3421 tgattttaag cgactttgat atcttcgtta aattcttaga taactcatca taattaacag
     3481 actctaacgc attaaaaata gcatcccgat caaccttact gaatttctta tccagtacat
     3541 tcttatactt ttcaaatgct gccagagctt catcaacacc ttgaattttc tttcctttag
     3601 acttttccgc taaatcctga gcaatttttg cgtatttttc tccatattgt tcggttatat
     3661 attgataaaa tccaaccata gtatcaacag catcttttat ctggctcttt tcaagagcat
     3721 cctgagcttc ttttaatttc tgctctgcct ctttaacctc agcggcctta ccatccctga
     3781 aactaacagc attcttaagt tcattttcaa ctgcggagta ctcagcctgc ttagcttcaa
     3841 gctgacgctg tagttttttc tgaacgtcac gccatcccgg aaatccggtg accttcacgt
     3901 caacactctt ctgagcgttt tcaagctctc ctgcaactct ggaaacagta gcttgcttac
     3961 tctgtacatc actttcagcc ttcgccagtt cagctttcac ctcagctaaa cgcttctctg
     4021 cttccgccac cgcctgtttc tcttcattaa gagtttcatg ttcagcgacc tctgcatttt
     4081 cacgagcctt ttcttcagct tccttctgtt tattcacatc accaatttta ctttgcaatg
     4141 tatttttata agaattaagt ttgctaatat ctgcatcaag ctgatttgat tttttttgca
     4201 actcatcaac atccctctca agatcagtga taccatgata agaatgatgc ttgaatactt
     4261 ttttcatctc ctcgattttc ttctgttttt cactaatctg tgtagcaatt ttattcttct
     4321 gcttttgttt ttcattaatt acattaccca ccaccttcga actcttatcc atatcactga
     4381 cctgagcatt cgttggtgca gtattaactg aagcagtgtt attgttgtta tttcctgagc
     4441 tttctgcaaa aagtgatggc atatcactaa ttaaagaatt aagaactctg gagacccctc
     4501 caaatggatt atcaaccaga gttgaattct cttctgtcat aacaatacca ttcatcaccg
     4561 gaaggccatc attattaatg acaacatcac cccacggagt cagatatgac tcaccggttt
     4621 tcatcacagt tgatgtagaa ccagatgaat ttgaatttcc ctgaccacca ttattaccat
     4681 gcccagagcc accaccccag tgaaccccac tattactatt attatttcca ccttgctgat
     4741 tcagattcgc ccctgttccc cccatagact caccagcagt tggtccatat ccacttagtt
     4801 ctttagccat aaattcctct ttgataatta aaacaataaa ttaaaaacaa tatactgtac
     4861 atataaccac tggttttatg tacagtaaaa cctactactc agtattgtcc atgtcaagag
     4921 catggatttt catttttgca ataaggatca cactatgggg aggcaggcat tgagaacgtc
     4981 gaaacagaac accggagcaa atcaggatga gatataaaac tgttggatca tgaaaaaacg
     5041 gagaacgatg tgagcaaatc accccgccat aaactgaaca aaacagacaa acgacttctc
     5101 gacacccttg ttgctgccgg atatgagcat gacaaagccc gtgacctcat ccagaaacag
     5161 gtttacacgc tgacactggc tgatcagcgt catgtggtca gtgaaatcag taatggtgtg
     5221 aatcccaccc aggcttactc ggcggtatac caggcaagac gcattcgcct cgcccgtaaa
     5281 tatctgaacg gtaaaaaggt tatggaagaa accggggaaa atacgccccc atcagcgtaa
     5341 ggatttcttt tgccgctcca gagactccag ttttttacgc aaatcctctc ttttttgggc
     5401 atctctggtg ccagccagct ctgctctcaa ctcatcgatc tgaagttgta tcttcagtct
     5461 gttactgaat attttctgtc tggcattaac atccgcaaca atgccgtttt ttgtcttctc
     5521 ggccttttgt tgaaaaacat tgctgtccgc atgactggca accgaagcag aaagaacact
     5581 aaaaagcagg actggcacac attttttcac gggattattc ctgactcatt gaccatcaaa
     5641 tcacattggg agtaaaccga cgtatgataa gagatactct tcggagatat aactccctga
     5701 gtatcaagat taaaaacgca aggagatgtt tatgagatct gccgctgcca ggctgcttct
     5761 gatacctctg ataacagcaa caatagctct tacaggatgc acaccaaaga ccagcctgga
     5821 acgacatacc cggcattatg tttatgcttc agatgatgga tttgatccta acttctacac
     5881 ccagaaagca gacaccatac gtatgatgct cccgttcttt cagcagttcc gggatatggg
     5941 gatgaaagac aaagcagccg gagtatcagc agaaacggca cagcaacgtg taaaagaatt
     6001 ccactcagaa aaattttttc actcactccg gagcacaaca gcctttgctg gcagaaaata
     6061 cacaaacagc gatatgcctt cgccgaaaaa aatgaaacta atggcagaca ccatttctgc
     6121 ggtttatctc gatggatacg agggcagaca gtaagggatt taccataatc ccttaattgt
     6181 acgcaccgct gaaatgcgtt cagcgcgatc acggctgctg acaggtaaaa atggcaacaa
     6241 accacccgaa aagctgccgc gatcgcacct gataaatttt aaccgtatgc atagctattc
     6301 agccatgtga ataacgctgg ttttgcctgc gtaaacctca tgacactgtt ttttttccat
     6361 cttttcagtt gatgacatac gcagacatcg cgggatgagg ctgaggaatg agcgcgatct
     6421 ggcaaagagg caaaacacag caacaaaaac gacacgccag aatcgcgccc ggatgcgttt
     6481 ttaacgcgtt ccggtaccat ctggcaacct cccggaacaa ctcaccgtca catccctatt
     6541 gacgggccac gccatacccg tgcttcccgt tcctgctctt catgccagga ccgcgcacgc
     6601 tcccgttcca ggcgtgcctg cctttcctgt tcatccctta tctgctgttc gtgataaata
     6661 accgactcaa gtggtccacc tgcccggcta atctctgcac ctgctgactc aagtcgtcgc
     6721 actgttccct cagttgcccg ttctcctgtc gtgtcagctc gaacatatgc tgcaaatccg
     6781 tgaaggcgct ctcccagtct ttcagccgct gcgtatagtc ctgctgcaat tgctctaagg
     6841 cgttcagtaa gtgcatttcc agctctgtca tactcactta ctccctgacc agtcttactg
     6901 cgttcttctt ctccaccgtc cagttgtttt cccccttcac cccggacggc aacaccagaa
     6961 atttcccgtt cctgccctcg tgatacgt
//
