LOCUS       NZ_CP054981             6647 bp    DNA     circular CON 02-MAY-2024
DEFINITION  Shigella flexneri strain STIN_92 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP054981
VERSION     NZ_CP054981.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13925548
            Assembly: GCF_022353685.1
KEYWORDS    RefSeq.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Che,Y., Xu,X., Yang,Y., Brinda,K., Hanage,W., Yang,C. and Zhang,T.
  TITLE     High-resolution genomic surveillance elucidates a multilayered
            hierarchical transfer of resistance between WWTP- and
            human/animal-associated bacteria
  JOURNAL   Microbiome 10 (1), 16 (2022)
   PUBMED   35078531
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Che,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2020) Civil Engineering, The University of Hong
            Kong, Pokfulam Road, Hong Kong 000000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: "Other" v. Unicycler
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology :: Illumina; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022353685.1-RS_2024_05_02
            Annotation Date                   :: 05/02/2024 05:06:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,265
            CDSs (total)                      :: 5,144
            Genes (coding)                    :: 4,880
            CDSs (with protein)               :: 4,880
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 264
            CDSs (without protein)            :: 264
            Pseudo Genes (ambiguous residues) :: 0 of 264
            Pseudo Genes (frameshifted)       :: 85 of 264
            Pseudo Genes (incomplete)         :: 182 of 264
            Pseudo Genes (internal stop)      :: 46 of 264
            Pseudo Genes (multiple problems)  :: 47 of 264
            Pseudo Genes (short protein)      :: 4 of 264
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP054981.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /strain="STIN_92"
                     /isolation_source="Shatin WWTPs_92 WWTPs influent"
                     /db_xref="taxon:623"
                     /plasmid="unnamed4"
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.4 N 114.2 E"
                     /collection_date="2018-12"
     gene            complement(338..661)
                     /locus_tag="HUZ60_RS26070"
                     /old_locus_tag="HUZ60_25715"
     CDS             complement(338..661)
                     /locus_tag="HUZ60_RS26070"
                     /old_locus_tag="HUZ60_25715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            725..916
                     /locus_tag="HUZ60_RS26075"
                     /old_locus_tag="HUZ60_25720"
     CDS             725..916
                     /locus_tag="HUZ60_RS26075"
                     /old_locus_tag="HUZ60_25720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1586..1744
                     /locus_tag="HUZ60_RS26420"
     CDS             1586..1744
                     /locus_tag="HUZ60_RS26420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531517.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     gene            2242..2469
                     /locus_tag="HUZ60_RS26080"
                     /old_locus_tag="HUZ60_25730"
     CDS             2242..2469
                     /locus_tag="HUZ60_RS26080"
                     /old_locus_tag="HUZ60_25730"
                     /inference="COORDINATES: protein motif:HMM:NF015489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WP_229471606.1"
                     /translation="MLYAIVISTILCPYSKYAIEYIAFNFIKKDFFERRKNLNNAPVAK
                     LNLFMLYNLLCLVLAIPFGLLGLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            complement(2466..4034)
                     /gene="cea"
                     /locus_tag="HUZ60_RS26085"
                     /old_locus_tag="HUZ60_25735"
     CDS             complement(2466..4034)
                     /gene="cea"
                     /locus_tag="HUZ60_RS26085"
                     /old_locus_tag="HUZ60_25735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WP_000447006.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            4294..4731
                     /locus_tag="HUZ60_RS26090"
                     /old_locus_tag="HUZ60_25740"
     CDS             4294..4731
                     /locus_tag="HUZ60_RS26090"
                     /old_locus_tag="HUZ60_25740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001405929.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     gene            4812..5231
                     /locus_tag="HUZ60_RS26095"
                     /old_locus_tag="HUZ60_25745"
     CDS             4812..5231
                     /locus_tag="HUZ60_RS26095"
                     /old_locus_tag="HUZ60_25745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(5549..5782)
                     /locus_tag="HUZ60_RS26100"
                     /old_locus_tag="HUZ60_25750"
     CDS             complement(5549..5782)
                     /locus_tag="HUZ60_RS26100"
                     /old_locus_tag="HUZ60_25750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137445.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(5789..6307)
                     /locus_tag="HUZ60_RS26105"
                     /old_locus_tag="HUZ60_25755"
     CDS             complement(5789..6307)
                     /locus_tag="HUZ60_RS26105"
                     /old_locus_tag="HUZ60_25755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067500.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     Typing          complement(1002..1089)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          1100..1658
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.544"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2..348)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.138"
                     /coverage="91.0"
                     /evalue="4.21e-70"
     Typing          complement(5446..6647)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.284"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggtctgtagc tccatgttcg gaatgacaaa attcagctca agccgtccct tgtcctggtg
       61 ctccacccac aggatgctgt actgattttt ttcgagaccg ggcatcagta cacgctcaaa
      121 gctcgccatc actttttcac gtcctcccgg cggcagctcc ttctccgcga acgacagaac
      181 accggacgtg tatttcttcg caaatggcgt ggcatcgatg agttcccgga cttcttccgg
      241 attaccctga agcaccgttg cgccttcgcg gttacgctcc ctccccagca ggtaatcaac
      301 cggaccactg ccaccacctt ttcccctggc atgaaattta actatcatcc cgcgccccct
      361 gttccctgac agccagacgc agccggcgca gctcatcccc gatggccatc agtgcggcca
      421 ccacctgaac ccggtcaccg gaagaccact gcccgctgtt caccttacgg gctgtctgat
      481 tcaggttatt tccgatggcg gccagctgac gcagtaacgg cggtgccagt gtcggcagtt
      541 ttccggaacg ggcaaccggc tcccccaggc agacccgccg catccatacc gccagttgtt
      601 taccctcaca gcgttcaagt aaccgggcat gttcatcatc agtaacccgt attgtgagca
      661 tcctctcgcg tttcatcggt atcattaccc catgaacaga aatccccctt acacggaggc
      721 atcagtgact aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac
      781 attaacgctg ctggagaagc tcaacgaact ggacgcagat gaacaggccg atatttgtga
      841 atcgcttcac gaccacgccg atgagcttta ccgcagctgc ctcgcacgtt tcggggatga
      901 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtgagcgga
      961 tgccgggagc tgacaagccc gtcagggcgc gtcagcaggt tttagcgggt gtcggggcgc
     1021 agccctgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacca tgcggcatca
     1081 gtgcggattg tatgaaaagt acgccatgcc gggtgtgaaa tgccgcacag atgcgtaagg
     1141 agaaaatgca cgtccaggcg cttttccgct tcctcgctca ctgactcgct acgctcggtc
     1201 gttcgactgc ggcgagcggt actgactcac acaaaaacgg taacacagtt atccacagaa
     1261 tcaggggata aggccggaaa gaacatgtga gcaaaagacc aggaacagga agaaggccac
     1321 gtagcaggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atagacgctc
     1381 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     1441 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     1501 cccttcggga agcgtggcgc tttctcatag ctcacgctgt tggtatctca gttcggtgta
     1561 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     1621 cttatccggt aactatcgtc ttgagtccaa cccggtaagg cacgccttaa cgccactggc
     1681 agcagccact ggtaaccgga ttagcagagc gatgatggca caaacggtgc tacagagttc
     1741 ttgaagtagt ggcccgacta cggctacact agaaggacag tatttggtat ctgcgctctg
     1801 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     1861 gttggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     1921 caagaagatc ctttgatctt ttctactgaa ccgcgatccc cgtcagttta gaagaggagg
     1981 atggtgcgat ggtccctccc tgaacatcac gtatatagtt agcctgacat ccaacaagga
     2041 ggtttatcgc gaatattccc acaaaaaatc ttttcctcat aactcgatcc ttataaaatg
     2101 aaaagaatat atggcgaggt ttaatttatg agcttaagat actacataaa aaatatttta
     2161 tttggcctgt actgcacact tatatatata taccttataa caaaaaacag cgaagggtat
     2221 tatttccttg tgtcagataa gatgctatat gcaatagtga taagcactat tctatgtcca
     2281 tattcaaaat atgctattga atacatagct tttaacttca taaagaaaga ttttttcgaa
     2341 agaagaaaaa acctaaataa cgcccccgta gcaaaattaa acctatttat gctatataat
     2401 ctactttgtt tggtcctagc aatcccattt ggattgctag gactttttat atcaataaag
     2461 aataattaaa tccctaacac ctcatttata gtattaagtt tattcttatc aatataggag
     2521 catagaattc ctgtaacaat agcaataccc caaataccta atgtagttcc agcaagcaag
     2581 ctaaaaagta aagcaacaac ataactcacc cctgcatctg cagctttctt ctctaatgta
     2641 agaaatagtg gcttccagtc acctgtatcc ttaattttta ggatatcaga taccacatca
     2701 tatccaaaag aaacatgccc cgtaatcttc aagtacttgg caaactgatc taaatgttta
     2761 gcccagtcat catacttcac cgatgccaac gcattaaaaa tagcatcacg atcggctttg
     2821 ctgaatttct tatttaaaac atccttgtat ttttcaaaag cagcgagagc ttcattcaca
     2881 ttgccgattt tcttaccttt agacttatca gcaagttcct gtgccatttt cgaatatttt
     2941 tcaccatatt tttcagtcag cgtttgataa aagctaactg ttgcatcaac agcatcctta
     3001 atctgtgaat taaggagatt attctgtgct tttttcaaat tttcttcagc ttcatgaaca
     3061 cgagcgatac cggcattacg attattactg acctgagaaa tagccttctg gatctgagtt
     3121 atatcagcat ttatccggtt aatacgtgtt tctgatgctg ttacctgttt ttgtttttct
     3181 tctctaatct taccggcccc aacccgtcgt ctggttgctt caaaaaaagg acggttctga
     3241 agcggatcat tggctcttgg tgatagtttt ttgaccagct catccagttc tttatattta
     3301 gcggatgcct gagccagttc atttcgtttt ccagcgagcg ttttcatttc tgcatcacgg
     3361 gcatggatac tggagcttaa acgagaattg agagtcttaa tctctccatc cattttcacc
     3421 acttcagatt gtgcagcaga aagttttttt tgggcgatct caacagcttt agcttcttca
     3481 ctcaatgcag ccagtcgttt ctcttcagct tcagccagtt tcaactggcg ttctgtttca
     3541 gccttctccc gttcaatctc tttacgtcgt tgttctgctt cctgaaaagc cttttctgct
     3601 gcttccgctt ctttacgggc tttttcttct gctttcgcaa ggcgcaaacg ctctgcttcc
     3661 gcctgcatag ctgcattatt agcatgagca agctctgttg ctgaaggcgt acgtgaggca
     3721 ttgtgacgaa gagcctcatt cacgatatcc ttcaggcgct gagtcagcgc atccctgttt
     3781 gcctttgctt tcgcctgtgc ttccgctgca gcttttgccc gggcagcctg ctctgcctgt
     3841 gttttcttta attgagcagt agaccattta gcagttgcat gaatagctgc agaactttca
     3901 cttttactgc ctccttttcc acctccgccg ccagagccac tcccgtcagg agtaccattc
     3961 aaaagagtaa taattacctg tcccttatca tcataaggaa caccatcttt atagtacgct
     4021 accgcggttt ccattataaa atcctctttg acttttaaaa caataagtta aaaataaata
     4081 ctgtacatat aaccactggt tttatataca gcataaaagc tacgccgctg cattttccct
     4141 gtcaagactg tggacttcca tttttgtgaa aacgatcaaa aaaacagtct ttcacaccac
     4201 gcgctattct cgcccgatgc cacaaaaacc agcacaaaca ttaccgttct cagacctcat
     4261 tatgttttac tgaaactatg agatgagaca tctatgggac actgtcactt tatggcatgg
     4321 cacacactcc gggacgcact aaaaatgaca ggcagatcgc gttcacagtt ttaccgtgat
     4381 atgcgcgcag gccttgtcag ttaccgtacc ggcagggacg gacgacggga gtttgaaacc
     4441 agtgaactga tccgggcata cggcgaatta aagcagaatg agacaccaga aaggcacagt
     4501 gagggacatg cagaaaatcc acatgatcag cagacagaac gcattctccg ggagctgaat
     4561 gagctgaaac aatgcctgac gctgatgctt gaggataaac aggcacagga tatggatcgc
     4621 agacgccagg aagcagaacg ggaacagcta caaaatgaga tagcccagct caggcaggca
     4681 ctggaactgg aaaagaaacg gggattctgg tccaggttgt tcggtcgctg aacgctgtca
     4741 gagactgatg ataaaatagt cttcggataa taactcaccg agaataaata ctttaaggta
     4801 gggagacact catgagacgt accggaaaca aactttgtct tatcgccatg ataacagcaa
     4861 cagtagctct cacagcctgt accccaaagg gcagcgtgga acaacatacc cggcattacg
     4921 tatatgcttc tgatgacggt tttgatccca acttttccac ccaaaaagcc gacacaacac
     4981 gaatgatggt gccttttttt cggcagttct gggatatggg agctaaagat aaagcgacag
     5041 gaaaatcacg gagtgatgtg caacaacgca ttcagcagtt tcacagccag gaatttttaa
     5101 actcactccg gggcacaact caatttgcgg gtactgatta ccgcagcaaa gaccttaccc
     5161 cgaaaaaatc caggctgctg gctgacacga tttctgcggt ttatctcgat ggctacgagg
     5221 gcagacagta agtggattta ccataatccc ttaattgtac gcaccgctaa aacgcgttca
     5281 gcgcgatcac ggcagcagac aggtaaaaat ggcaacaaac cacccgaaaa actgccgcga
     5341 tcgcgcctga taaattttaa ccgtatgaat acctatgcaa ccagagggta caggccacat
     5401 tacccccact taatccactg aagctgccat ttttcatggt ttcaccatcc cagcgaaggg
     5461 ccatccagcg tgcgttcctg tatttccggc tgacgctccc gttccaggga taacacatgt
     5521 tcgcgctcct gtatcagccg ttcctctctt atctccagtt ctcgctgtat aactggctca
     5581 agcgttctgt ctgctcgctc aagtgctgca cctgctgact caactgcatg acccgctcgt
     5641 tcagcatcgc gttgtcccgt tgcgtaagcg aaaacatctt ctgcaattcc acgaaggcgc
     5701 tctcccattc gctcagccgc tgcatatagt cctgttgcag ctgctctaag gcgttcagca
     5761 aatgtgtttc cagctctgtc actctgtgtc actccttcag atgtacccac tctttcccct
     5821 gaaagggaat cacctccgct gatttcccgt acggaaggac aaggaatttc ctgttcccgt
     5881 cctgcacaaa ctccacgccc catgtcttcg cgttcagttt ctgcaatgtc tcttcctgct
     5941 tcctgatttc ttccaggttc gcctgtatcc tccctccaag ataccagagc gtcccgccac
     6001 tcgcggtaaa caggagaaag actatcccca gtaacatcat gcccgtattc cctgccagct
     6061 ttaacacgtc cctcctgtgc tgcatcatcg cctctttcac cccttcccgg tgtttttcca
     6121 gcgattcctc tgtcgaggct gtgaacaggg ctatagcgtc tctgattttc gtctcgtttg
     6181 atgtcacagc ctcgcttaca gattcgccga gcctcctgaa ctcgttgttc agcattttct
     6241 ctgtagattc ggctctctct ttcagctttt tctcgaactc cgcgcccgtc tgcaaaagat
     6301 tgctcataaa atgctccttt cagcctgata ttcttcccgc cgttcggatc tgcaatgctg
     6361 atactgcttc gcgtcaccct gaccacttcc agccccgcct cagtgagcgc ctgaatcaca
     6421 tcctgacggc cttttatctc tccggcatgg taaagtgcat ctatacctcg cgtgacgccc
     6481 tcagcaagcg cctgtttcgt ttcaggcagg ttatcaggga gtgtcagcgt cctgcggttc
     6541 tccggggcgt tcgggtcatg cagcccgtaa tggtgattca ccagcgtctg ccaggcatca
     6601 attctaggcc tgtctgcgcg gtcgtagtac ggctggaggc gttttcc
//
