LOCUS       NZ_CP054976             5252 bp    DNA     circular CON 02-MAY-2024
DEFINITION  Klebsiella pneumoniae strain STIN_93 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP054976
VERSION     NZ_CP054976.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13925549
            Assembly: GCF_022353665.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5252)
  AUTHORS   Che,Y., Xu,X., Yang,Y., Brinda,K., Hanage,W., Yang,C. and Zhang,T.
  TITLE     High-resolution genomic surveillance elucidates a multilayered
            hierarchical transfer of resistance between WWTP- and
            human/animal-associated bacteria
  JOURNAL   Microbiome 10 (1), 16 (2022)
   PUBMED   35078531
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5252)
  AUTHORS   Che,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2020) Civil Engineering, The University of Hong
            Kong, Pokfulam Road, Hong Kong 000000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: "Other" v. Unicycler
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology :: Illumina; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022353665.1-RS_2024_05_02
            Annotation Date                   :: 05/02/2024 05:01:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,400
            Genes (coding)                    :: 5,288
            CDSs (with protein)               :: 5,288
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 48 of 112
            Pseudo Genes (incomplete)         :: 62 of 112
            Pseudo Genes (internal stop)      :: 23 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP054976.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5252
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="STIN_93"
                     /isolation_source="Shatin WWTPs_93 WWTPs influent"
                     /db_xref="taxon:573"
                     /plasmid="unnamed4"
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.4 N 114.2 E"
                     /collection_date="2018-12"
     gene            join(5123..5252,1..1226)
                     /locus_tag="HUZ61_RS27540"
                     /old_locus_tag="HUZ61_27500"
     CDS             join(5123..5252,1..1226)
                     /locus_tag="HUZ61_RS27540"
                     /old_locus_tag="HUZ61_27500"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_014386473.1"
                     /translation="MFVYLTIENFRSVKEKSTLNLSASGSSNHLTTHIYKNDGMNVGTL
                     MSAGIYGANASGKSNVLMAFEAIKFIATESGDLKEGKRIPCYEPYALSSATNEAPVKFE
                     AEFYSVEGNRFSYEVAFIKNRILFESLDYYPSRVKANLFTRDESDTWETIKFGGHYKGG
                     IKKIPFFPNNSYLAKAGNNAASPDIIKEAYNFFRKGIRHIGLNEKIRISSFGEREDVVN
                     ETAKVLCMVDTGVSGISVREVEAEIPIKMDEEIPQEIKEIIEEDYKYRYMFSHPTDEGG
                     SVSFPMGRESEGTQKLFEIIPLIRSAFNDSMVVIVDELDNSLHPHIADLIVKLFNDPDV
                     NKKGSQLVFSTHNMQLMAPEKMRRDQIWFCEKNKGASSLYSLDDFDKKKIKTTTPYAAW
                     YDEGRFGGVPDINYLKVASFISGDISLVMPDIDVKELSDGFFEEFDGDLSDE"
                     /name="ATP-binding protein"
     gene            1219..1839
                     /locus_tag="HUZ61_RS27545"
                     /old_locus_tag="HUZ61_27505"
     CDS             1219..1839
                     /locus_tag="HUZ61_RS27545"
                     /old_locus_tag="HUZ61_27505"
                     /inference="COORDINATES: protein motif:HMM:NF025084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB family protein"
                     /protein_id="WP_023312615.1"
                     /translation="MSKKHGNNKKVPQVMYIYCEGEKTEPLYLEAYISDRSKRTLSVFK
                     IPKTRKNTPEQLVDEAIKKKNSSSSAEGDVFWVVYDQEHLTPSSISCHQKAWNKANRHG
                     INIAISCVCFELWLLLHFGFTTKDFSSYDNLMSSSPLKKLITNYDKGSKLTYLAIRERI
                     GTAKDNARKLDIYAKTNYKNTHEPYLFPCYTSFHELLKAIDDF"
                     /name="RloB family protein"
     gene            complement(1992..2153)
                     /locus_tag="HUZ61_RS27550"
                     /old_locus_tag="HUZ61_27510"
     CDS             complement(1992..2153)
                     /locus_tag="HUZ61_RS27550"
                     /old_locus_tag="HUZ61_27510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023144844.1"
                     /translation="MHEPPVQSDRCAFSGNYHLESNPERHGSSPLAAAIGNGLEEVSLE
                     VLRQLRLN"
                     /name="hypothetical protein"
     gene            2791..3111
                     /locus_tag="HUZ61_RS27555"
                     /old_locus_tag="HUZ61_27515"
     CDS             2791..3111
                     /locus_tag="HUZ61_RS27555"
                     /old_locus_tag="HUZ61_27515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_014386475.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGRLPSI
                     SPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAVLEKGGDD
                     DR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3320..>3751
                     /locus_tag="HUZ61_RS27560"
                     /pseudo=""
     CDS             3320..>3751
                     /locus_tag="HUZ61_RS27560"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3789..4274
                     /locus_tag="HUZ61_RS27565"
                     /old_locus_tag="HUZ61_27525"
     CDS             3789..4274
                     /locus_tag="HUZ61_RS27565"
                     /old_locus_tag="HUZ61_27525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_013631087.1"
                     /translation="MSSLLALAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVKAELSASA
                     KRISDAISAHEQGMTAAMQSNRLSVLRMVGRTWLTITLVSVLLIATSGSILWWQGQQIT
                     GNYQTIRAQERTQAMLSEKNHGVQLSLCGEKKLSCVKVNPKAGAYGEEGNWMVLERK"
                     /name="MbeB family mobilization protein"
     gene            4278..4490
                     /locus_tag="HUZ61_RS27570"
                     /old_locus_tag="HUZ61_27530"
     CDS             4278..4490
                     /locus_tag="HUZ61_RS27570"
                     /old_locus_tag="HUZ61_27530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137423.1"
                     /translation="MTELEKQLLTALEQLHQDYSQRLDEWESAFAEWQKMSGLMQRENA
                     ALNERVTRLSQQVANLSRQLQRLSQ"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          2505..2677
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="84.659"
                     /coverage="100.0"
                     /evalue="9.74e-41"
     Typing          complement(2041..2558)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.282"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          3102..4606
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.215"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cgctcatgag tgctgggatt tatggtgcta atgcgtctgg taaatctaac gttctcatgg
       61 cttttgaagc tataaaattt atcgcaacag aaagcggtga tttgaaagaa ggtaaacgga
      121 ttccctgcta tgagccatat gctctttctt cggctacgaa tgaggctcct gtaaagtttg
      181 aggctgaatt ttattctgtt gaggggaatc ggttcagtta tgaagttgct tttattaaaa
      241 atcggatttt atttgaatca ttagactatt atccatctag ggttaaagca aatttattta
      301 ctagagatga gagcgacacc tgggaaacaa tcaagtttgg tgggcattat aaaggaggta
      361 ttaaaaaaat accattcttc cctaataatt catacttagc gaaagcaggt aataatgccg
      421 catcaccaga cataattaaa gaagcttata acttttttag aaagggaatt agacatatag
      481 gtttaaatga aaagataaga atatcttctt ttggggagag ggaggacgtc gtaaatgaaa
      541 cggccaaggt tttatgcatg gttgacactg gtgtttccgg tatatctgta cgggaggttg
      601 aggcggaaat accaataaaa atggatgagg aaatccctca agaaataaag gagataattg
      661 aagaagatta taaatatcgt tatatgttct ctcatcctac tgatgaaggg gggagtgttt
      721 ctttccctat ggggagagag tctgaaggca cccaaaaatt atttgaaatc atacctctaa
      781 ttagatctgc gtttaatgat agtatggttg taattgttga tgagttagat aatagcttgc
      841 acccgcatat tgctgacttg attgttaagt tatttaatga tcctgatgtc aataaaaaag
      901 gctcacaact tgtattctca acccataata tgcagttaat ggctccagag aaaatgagaa
      961 gagatcagat atggttttgt gaaaaaaata aaggtgcgtc gtctctttat agcttagatg
     1021 attttgataa gaaaaagata aaaacaacaa caccttacgc agcttggtat gatgagggta
     1081 ggtttggtgg ggttcccgac ataaactact taaaggtagc ctcatttata tctggtgata
     1141 tatccttggt aatgccggat attgacgtca aagaattgtc tgatggtttt tttgaagagt
     1201 ttgacgggga tctttctgat gagtaaaaaa catggaaata ataagaaagt cccacaagta
     1261 atgtatatat actgtgaggg tgagaaaacc gaaccattat accttgaggc ttatatttca
     1321 gacagatcta aaagaacttt aagtgtattt aaaataccta aaacaagaaa aaatacacct
     1381 gagcagttgg tggatgaggc gataaagaaa aagaattcat catcttccgc agaaggtgac
     1441 gtcttttggg ttgtttatga tcaagagcat ctgacgcctt cgtctatttc atgtcatcaa
     1501 aaggcatgga ataaagctaa taggcatggg ataaatatag caatatcctg tgtttgtttt
     1561 gaattatggt tattgttgca tttcggcttc acaaccaaag atttttcgtc gtatgataat
     1621 ttgatgtcta gctccccgct aaaaaaatta ataactaact atgataaagg tagtaaacta
     1681 acttaccttg ctataagaga gaggattggt actgcgaaag ataacgcaag aaagctagat
     1741 atttacgcta aaactaatta taaaaacacg cacgaacctt atctatttcc atgttataca
     1801 tcattccatg agctacttaa ggcgattgac gacttttgaa gtaatccatt gaccatggct
     1861 tattacaggc tgtaatctat gaaaaataaa aaccaccgct accaacggtg gtttttggaa
     1921 ggtacatgag ctaccaactt atgtaccggg taactggctt agagggagcg cagtcaccaa
     1981 aaattgtcct ttcagtttag ccttaactgg cgcagaactt caagactcac ttcctctaat
     2041 ccgttaccaa tggctgctgc cagtggcgat gaaccgtgtc tttccgggtt ggactcaaga
     2101 tgatagttac cggagaaggc gcagcggtcg gactgaacgg ggggttcgtg catacagtcc
     2161 agcttggagc gaactgccta cccggaactg agtgtcaggc gtggaatgag acaaacgcgg
     2221 ccataacagc ggaatgacac cggtaaaccg aaaggcagga acaggagagc gcacgaggga
     2281 gccaccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc accactgatt
     2341 tgagcgtcag atttcgtgat gcttgtcagg gggggcggag cctatggaaa aacggcagcg
     2401 gcgcatcctt tttctgcccc gtttgtcatg ccatatgcct tcgctcttct ccgtgtgcag
     2461 gtaacgcccg ccgcagccga acggcaggag cggcagcgac tgagtgagcg aggaggcgtc
     2521 atatcctctg aacttccatt ttctgcctta cgcccgcagc tgcattttcc tctgtcacat
     2581 gaagcacttt cctgattacc gcactcatgc caacataata agccagtata cactccgcta
     2641 gcgctacgtg actggttcag ggctgcgccc cgaaacccgc taaaagcgac tgacgcgcct
     2701 gcggcttgtc caacaccgtg ccgaccggga aacatttccc gcccgtcccg gtgttatcag
     2761 gaggctactc tggcagacaa acgcaacaag atgctcacga tgtgggtgac ggaagacgaa
     2821 caccggcggc tgctcgagcg gtgcgacggc aagcagctgg cggcgtggat gcgtcagacc
     2881 tgcctcgatg agaagccggc gcgtgccggc agactgccgt cgatatcgcc ggcgctgctg
     2941 cgccagcttg ccggtatggg gaacaacctg aaccagatcg cccggaaagt gaatgccggt
     3001 ggtgccggtc atgaccgggt gcagattgtg gccgcactga tggcaatcga cgcgggactg
     3061 gagcggctgc ggcatgcggt gctggaaaaa gggggggatg atgatcgtta aatttcaccc
     3121 ccgggggcgc ggtggtggtg ccggtccggt ggattacctg ctgggaaaag accggcagcg
     3181 cgagggggcg accgtgctgc agggaaaacc ggaggaagtc cgggagctta tcgatgcctc
     3241 gccgtatgcg aaaaagtaca cctccggcgt gctgtcgttc gcggaggctg aactgccgcc
     3301 cgggcagcgg gagcagatta tggccagctt tgagcgggtt ctgatgcccg ggctcgataa
     3361 agaccagtac agcattctgt gggtggagca caccgacaag gggcggctgg agctgaattt
     3421 cctgatcccc aacactgaac tgctgaccgg gaaacgcctg cagccgtact acgatcgcgc
     3481 cgaccggccg cgcatcgatg cctggcagac cgtggtgaac ggcaggctgg ggctgcatga
     3541 tccaaacgcc ccggagaacc gacggttact ggtcacgccg tccgcactgc cggaaacgaa
     3601 gctggaagcc gctcaggcga ttacacgtgg gttgctggcc ttagcctcat ccggggagct
     3661 caaaacgcgc gaggacgtca caggggcgct cacagcggca ggttttgagg tggtccgcac
     3721 cacaaaaaac agtatcagca ttgccgaccc ggacgggggg cgaaacatcc gactgaaggg
     3781 agccatttat gagcagtctt ttagcgctgg cgaaggactt ggagcagcaa tcgaaagcgc
     3841 agcagcagag taccggcgag atgctgaaag ccgcattcag cgagcacgag cagtctgtaa
     3901 aagcggaact gagcgcaagc gcgaagagaa tcagcgacgc catcagcgcc cacgagcagg
     3961 gtatgaccgc ggcgatgcag tcgaaccggc tgagcgtgct gcgtatggtc ggccggacgt
     4021 ggctgaccat caccctggtc tcggtactgc tgatcgccac gagcgggagc attctgtggt
     4081 ggcaggggca gcagatcacc gggaattacc agactatccg ggcgcaggag cggacgcagg
     4141 ccatgctgtc ggagaagaac cacggcgtgc agctgtcgct ctgcggggag aagaaactgt
     4201 cctgcgtcaa agtgaacccg aaagcgggag cttacgggga ggaagggaac tggatggtac
     4261 tggagaggaa atagcccatg acggaactgg aaaaacagtt gctgaccgca ttagagcagc
     4321 tacatcagga ctactcgcaa aggctggacg agtgggagag cgccttcgcg gaatggcaga
     4381 agatgtctgg tcttatgcaa cgggagaacg cggcgctgaa cgagcgcgtc acgcgcttga
     4441 gtcagcaggt ggcgaacttg agcaggcagt tacagcgttt gagccagtag tgcagcggca
     4501 tgaaatggcg gtggccgccg aacgggcata cgagcagaaa cagcatgaaa aagagctggc
     4561 ggaaacccgt tcccggcagc ggagctatga cggtccgacg ctgggctgac tgcgcgcatc
     4621 cagtgacaaa ccccaagaag aagggctcat aaatttcccg ctaaatacgg tcgtgatgtt
     4681 tccatcacag cgtatacaac ggagggatta tgagactgtt taacgtgatt gagacggtgc
     4741 tgaattgcct gtacatggcg ctggagatct gggactggct ggagaagcat tttcctgttt
     4801 ttaaggcgtt catccggatt ctggcctgaa atcagaccgt atgcatctgg ctgcatgacc
     4861 atgcagccgg gtaaaattta tctgtcacga acggggcggt gtatcgggtg gtttgttgcc
     4921 ggttttacct gactgccgcc ccgtttgtgg cgaacctgtc cggagcggtg cgggcaacag
     4981 atgttatggt aaatagcgtg taattataaa tagcacaacg ttttttaagg aagatgttgt
     5041 tccaaaatta aaacatatag gatatctttc ctatgattga tttttcgctt ttttggcgaa
     5101 aaatccccgt taggaggtat gcatgtttgt ttatttgact attgaaaatt ttaggtcggt
     5161 taaggaaaaa tcgacattaa acttgagcgc atccggttct agtaaccatt taacaactca
     5221 tatctataaa aatgatggaa tgaatgttgg aa
//
