LOCUS       NZ_CP054971             2294 bp    DNA     circular CON 02-MAY-2024
DEFINITION  Klebsiella pneumoniae strain STIN_94 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP054971
VERSION     NZ_CP054971.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13925550
            Assembly: GCF_022353645.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Che,Y., Xu,X., Yang,Y., Brinda,K., Hanage,W., Yang,C. and Zhang,T.
  TITLE     High-resolution genomic surveillance elucidates a multilayered
            hierarchical transfer of resistance between WWTP- and
            human/animal-associated bacteria
  JOURNAL   Microbiome 10 (1), 16 (2022)
   PUBMED   35078531
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Che,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2020) Civil Engineering, The University of Hong
            Kong, Pokfulam Road, Hong Kong 000000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: "Other" v. Unicycler
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology :: Illumina; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022353645.1-RS_2024_05_02
            Annotation Date                   :: 05/02/2024 05:07:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,606
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 53 of 125
            Pseudo Genes (incomplete)         :: 78 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 24 of 125
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP054971.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="STIN_94"
                     /isolation_source="Shatin WWTPs_94 WWTPs influent"
                     /db_xref="taxon:573"
                     /plasmid="unnamed4"
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.4 N 114.2 E"
                     /collection_date="2018-12"
     gene            join(1388..2294,1..38)
                     /locus_tag="HUZ62_RS27960"
                     /old_locus_tag="HUZ62_27825"
     CDS             join(1388..2294,1..38)
                     /locus_tag="HUZ62_RS27960"
                     /old_locus_tag="HUZ62_27825"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="WP_000887055.1"
                     /translation="MLDRLESEILADRVSEESRRWLASCGLTVEQMKNQMDPVYTPARK
                     IHLYHCDHRGLPLALISTEGATAWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEESG
                     LYYNRHRYYDPLQGRYITQDPIGLKGGWNLYGYQLNPISDIDPLGLSMWEDAKSGACTN
                     GLCGTLSAMIGPDKFDSIDSTAYDALNKINSQSICEDKEFAGLICKDNSGRYFSTAPNR
                     GERKGSYPFNSPCPNGTEKVSAYHTHGADSHGEYWDEIFSGKDEKIVKSKDNNIKSFYL
                     GTPSGNFKAIDNHGKEITNRKGLPNVCRVHGNM"
                     /name="DUF4329 domain-containing protein"
     gene            10..393
                     /gene="yhhH"
                     /locus_tag="HUZ62_RS27965"
                     /old_locus_tag="HUZ62_27830"
     CDS             10..393
                     /gene="yhhH"
                     /locus_tag="HUZ62_RS27965"
                     /old_locus_tag="HUZ62_27830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417940.2"
                     /note="This BlastRule distinguishes YhhH from its paralog
                     YbbC. Both are named for founding members from Escherichia
                     coli K-12, and belong Pfam family PF15631; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhhH"
                     /protein_id="WP_001271686.1"
                     /translation="MSAEFMVICKKILFRNCVIVSLFVFTYNTWAQCNNNIKIMRKYES
                     EGKYTVRNLVKNKAIALELAEIYVKNRYGQDAAEEEKPYEITELTTSWVVEGTIHSDQI
                     AGGVFIIEIGKNDGRILNFGHGK"
                     /name="yhhH"
     gene            498..1340
                     /locus_tag="HUZ62_RS27970"
                     /old_locus_tag="HUZ62_27835"
     CDS             498..1340
                     /locus_tag="HUZ62_RS27970"
                     /old_locus_tag="HUZ62_27835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="WP_000072855.1"
                     /translation="MSNTYQKRKASKEYGLYNQCKKLNDDELFRLLDDHNSLKRISSAR
                     VLQLRGGQDAVRLAIEFCSDKNYIRRDIGAFILGQIKICKKCEDNVFNILNNMALNDKS
                     ACVRATAIESTAQRCKKNQIYSPKIVEQSQITAFDKSTNVRRATAFAISVINDKATIPL
                     LINLLKDPNGDVRNWAAFAININKYDNSDIRDCFVEMLQDKNEEVRIEAIIGLSYRKDK
                     RVLSVLCDELKKNTVYDDIIEAAGELGDKTLLPVLDTMLYKFDDNEIITSAIDKLKRS"
                     /name="HEAT repeat domain-containing protein"
ORIGIN
        1 gattacctaa tgtctgcaga gttcatggta atatgtaaaa aaatattgtt taggaactgt
       61 gtcattgtat ctttgtttgt ttttacatac aacacatggg cgcagtgtaa taataatatt
      121 aaaataatgc gcaagtatga aagtgaaggt aaatataccg ttagaaattt ggttaaaaat
      181 aaagctatag cattggaatt agctgagata tatgttaaga atcgttatgg acaggatgcc
      241 gcagaagaag aaaaaccata cgaaatcact gagttaacaa caagttgggt tgttgaaggt
      301 accattcact cagaccaaat tgctggtggg gtttttatta tagaaatagg caaaaatgat
      361 gggagaattc tgaattttgg ccacggaaaa taagtatgta aggataatca aacatcgtgt
      421 gcgctgatgg cagagtaggt ggaggactcc agacagtcaa acgatagaaa aagatagcct
      481 ttatggaggt tcctgcaatg tcaaatacat accagaaaag aaaggcaagt aaagagtatg
      541 gtttatataa tcaatgtaag aaactaaatg atgatgaatt atttcgctta cttgatgatc
      601 acaattcctt gaaaaggatt tcatctgcca gagtattaca gttaagaggt gggcaagacg
      661 ctgttagatt ggcaattgag ttctgctctg ataaaaatta tatccgtaga gatatcggag
      721 catttatact cgggcaaata aaaatttgca aaaaatgcga agataatgtt tttaatattt
      781 tgaacaatat ggcattgaat gataagagcg cttgcgttcg agctacggca atcgagtcaa
      841 cggctcagcg atgcaagaaa aaccaaattt attcacctaa aatagtagaa caatctcaaa
      901 ttactgcttt tgataaatcg actaatgtca gacgtgctac agcatttgct atttctgtta
      961 tcaatgataa agcaacaatt ccactattga ttaatctgtt aaaagatcca aatggagatg
     1021 tcagaaactg ggccgcattt gcaataaata tcaataaata tgataatagt gatattaggg
     1081 attgttttgt ggagatgctt caggataaaa atgaggaagt ccgtattgaa gcaataatcg
     1141 gactttccta cagaaaagat aaaagggttt tatctgtttt atgcgatgag ttaaaaaaaa
     1201 atactgttta tgatgatatc attgaagctg cgggtgaatt aggtgataaa acgctacttc
     1261 ctgttttaga tactatgttg tacaagtttg atgacaatga aattataact tccgctattg
     1321 ataagctgaa gcgttcatag cgtgagttgc ctatgcacag tgggggattc ccgccggcac
     1381 ggtgcagatg ctcgaccggc tggaaagtga aatcctggct gaccgggtga gtgaggaaag
     1441 ccgccgctgg ctggcatcgt gcggcctgac cgtggagcag atgaaaaacc agatggaccc
     1501 ggtgtacacg ccggcgcgaa aaatccacct gtaccactgc gaccatcgcg gcctgccgct
     1561 ggcgctcatc agcacggaag gggcaacagc gtggtgcgca gaatatgatg aatggggcaa
     1621 cctgctgaat gaagagaacc cgcatcagct gcagcagctt atccggctgc cggggcagca
     1681 gtatgatgag gagtccggcc tgtattacaa ccgccaccgc tattatgacc cgctgcaggg
     1741 gcgatatatc actcaggatc cgattggact gaagggggga tggaacctgt atggatatca
     1801 attgaatccg atatcagaca tcgaccccct gggtttatct atgtgggagg atgcaaaatc
     1861 gggggcatgt actaatggtc tttgcggcac actatccgct atgataggtc cagataaatt
     1921 tgattctata gatagcaccg catatgacgc cttaaataaa ataaatagcc aatctatttg
     1981 cgaagataaa gagttcgctg gtttaatatg taaggataat agtggcagat atttctcaac
     2041 agcacctaac cgaggagaaa gaaaaggatc atatccattc aatagccctt gccctaatgg
     2101 tactgagaaa gtatcagctt atcatactca tggtgcagat agtcatggag aatattggga
     2161 cgaaatattt tcaggtaaag atgagaaaat agttaaaagt aaagataaca atatcaagtc
     2221 attttattta ggtacgccca gtggtaattt taaagcaata gataaccacg ggaaggaaat
     2281 aacaaacaga aaag
//
