LOCUS       NZ_CP054970             4626 bp    DNA     circular CON 02-MAY-2024
DEFINITION  Klebsiella pneumoniae strain STIN_94 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP054970
VERSION     NZ_CP054970.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13925550
            Assembly: GCF_022353645.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4626)
  AUTHORS   Che,Y., Xu,X., Yang,Y., Brinda,K., Hanage,W., Yang,C. and Zhang,T.
  TITLE     High-resolution genomic surveillance elucidates a multilayered
            hierarchical transfer of resistance between WWTP- and
            human/animal-associated bacteria
  JOURNAL   Microbiome 10 (1), 16 (2022)
   PUBMED   35078531
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4626)
  AUTHORS   Che,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2020) Civil Engineering, The University of Hong
            Kong, Pokfulam Road, Hong Kong 000000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: "Other" v. Unicycler
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology :: Illumina; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022353645.1-RS_2024_05_02
            Annotation Date                   :: 05/02/2024 05:07:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,606
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 53 of 125
            Pseudo Genes (incomplete)         :: 78 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 24 of 125
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP054970.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4626
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="STIN_94"
                     /isolation_source="Shatin WWTPs_94 WWTPs influent"
                     /db_xref="taxon:573"
                     /plasmid="unnamed3"
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.4 N 114.2 E"
                     /collection_date="2018-12"
     gene            complement(join(4031..4626,1..988))
                     /locus_tag="HUZ62_RS27940"
                     /old_locus_tag="HUZ62_27810"
     CDS             complement(join(4031..4626,1..988))
                     /locus_tag="HUZ62_RS27940"
                     /old_locus_tag="HUZ62_27810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951932.1"
                     /GO_function="GO:0019239 - deaminase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-phage dCTP deaminase"
                     /protein_id="WP_223351761.1"
                     /translation="MSSANEDISGRDSIDQITSRQSMDIIIGFCGAVGSGIKSIVDVTE
                     EELEDLGYEVYRIRVSDLMQKLFSEWCGDTSTPFKRYNSLQDFGDSLRCKYYPHILAEA
                     VIHEIKIEKNLEGKSKELKKRAFLIDQLKHQDEIELLRMVYQHNFYLLGVIRSETERKR
                     NLRDEGLSKSEVDIIIHHDRKSEHPYGQQTAKAILDADYFVKNNQGQRAHLKSKVKRFL
                     GLIHGLNGLTPNAHEKGMYAAHSASLQSACLSRQVGAAILDIKGNLIAVGRNDVPKFNG
                     GLYSADDGVNDHRCVYKGGKCYNEVRKLKIKDKIQQILLSDKVKELSVQLTQEQAEKVA
                     GIIYEGTPVSSIIEYSRSIHAEMDAITSLARLGNGGFEDKVLYTTTFPCHNCARHIVAV
                     GINKVVYIEPYEKSLALELHDDAITEVNESGKVLFESFEGVSPRRYHKFFFSTDERKDS
                     KGNAEKYSTKYKNHIDIQFIDSYVDFENRVADIFISNTKTDAEAQVAVPSGTPVNPLLP
                     PIPPTPPTSV"
                     /name="anti-phage dCTP deaminase"
     gene            complement(1043..1225)
                     /locus_tag="HUZ62_RS27945"
                     /old_locus_tag="HUZ62_27815"
     CDS             complement(1043..1225)
                     /locus_tag="HUZ62_RS27945"
                     /old_locus_tag="HUZ62_27815"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_087523613.1"
                     /translation="MSKELSLNRISNRVSSSLKEGVVHERVVHGNVYDLELELLRRKRE
                     EAIKRIIEDSKSLKW"
                     /name="hypothetical protein"
     gene            complement(1585..1971)
                     /locus_tag="HUZ62_RS27950"
                     /old_locus_tag="HUZ62_27820"
     CDS             complement(1585..1971)
                     /locus_tag="HUZ62_RS27950"
                     /old_locus_tag="HUZ62_27820"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001598756.1"
                     /translation="MRAFNYSPRYANAVRVLNRLLDKEFLSAEEIRLYDDQGRVIEYIN
                     NRLLVPVLSMKISRDGEYVRVWYLSQEAIDDYYNRREYQFSQQRHAVAVARQHRKASTA
                     ARLLLSLGETVPESIRMILAANDD"
                     /name="hypothetical protein"
     gene            3713..3826
                     /locus_tag="HUZ62_RS27955"
     CDS             3713..3826
                     /locus_tag="HUZ62_RS27955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071594735.1"
                     /translation="MHEPPVQSDRCACPGNYRLESNPRRHDKAPLAAATGN"
                     /name="replication initiation protein"
     Typing          3441..3830
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.164"
                     /coverage="91.0"
                     /evalue="0.0"
ORIGIN
        1 gaacagacaa ctccttaacc ttatcagaaa gcaatatttg ctgtatttta tcttttattt
       61 ttaattttct tacttcattg taacatttgc cacctttgta gacacagcga tgatcattca
      121 ctccatcatc agcactataa aggccaccgt taaatttagg aacatcattg cgcccaacgg
      181 caattaaatt tccttttata tctaaaatag cagcacctac ctgtctggaa agacatgcag
      241 attgtaaaga ggcagaatga gctgcgtaca tgcccttctc atgcgcgttt ggcgtaaggc
      301 cattaagccc atgaattaga ccaagaaagc gtttaacttt agatttaagg tgagctctct
      361 ggccttgatt atttttaaca aaatagtcag catcaagtat agcttttgct gtttgctggc
      421 cataagggtg ctcagacttc ctgtcatgat ggatgattat atcaacttca gatttgctta
      481 aaccctcatc tcgaagattt ctttttcttt cagtctcgct cctaataaca cctaataaat
      541 agaaattatg ttgatagacc attctaagca actcaatttc atcttggtgc tttagttgat
      601 ctataaggaa agctcgtttc tttaactctt ttgattttcc ttctagattt ttttctatct
      661 ttatttcatg aattacagct tcagctagta tgtgtgggta gtacttacat cttaaggaat
      721 caccaaaatc ctgcaaggaa ttatatcttt tgaatggagt agatgtatcc ccacaccact
      781 cagaaaaaag tttctgcata agatcgctaa ctcttatcct atatacttcg tatcctaaat
      841 cttccagttc ttcttcggtt acatctacaa ttgacttaat accggaacca acagccccac
      901 agaaacctat aataatatcc atggattgcc tagatgtaat ctgatctata ctatcacgac
      961 cagaaatatc ttcattggca ctactcattg gagtgctctt tttttccttt aacttcctag
     1021 ctgtgctcat aacgcctctc aatcaccatt ttaatgattt agaatcttca attatccttt
     1081 ttatagcttc ttctcttttg cgccttaata actccaactc caaatcataa acatttccat
     1141 gaaccactct ttcatgaact accccctctt tcaaagagga cgaaactcta ttggaaatac
     1201 gatttagtga tagctctttg ctcatatttt ctcctgcgaa acgcatggat tttactgtta
     1261 tctttataac aggcaaccac aaacaggcgt aatgcgcaga agtttttttt gcttagaaca
     1321 ccaggcaaaa aagagtgaaa actacacaag tctatgaaat aaataaaaaa gctcgcatat
     1381 tgcgagcttt ttagtctagt ttaagaccta aacatcgtgc ttaagcactc tctccctgac
     1441 tgcttcatag accagatgaa gcccaaaaca aatcatggcg agaggcactc cccattcaac
     1501 gtttccagga agaaacccca agctctctaa taggtagaga aagccagcaa aaacaacaaa
     1561 taaccccaga aacataatat cctcctaatc atcattagca gcaagaatca tacggatact
     1621 ttctgggaca gtttcaccta gcgataaaag caaacgagcg gccgtagagg cttttctatg
     1681 ctgacgagca acagcgacag catgcctctg ctgactaaac tgatactcac gacggttgta
     1741 atagtcatca atggcttctt gagacaaata ccagacacga acatactcac catcgcggct
     1801 gatcttcatg ctcaaaacag gaacaagaag cctgttgttg atgtactcaa tcacccgccc
     1861 ttgatcatcg taaaggcgaa tttcttcagc actcagaaac tctttatcga gaaggcggtt
     1921 taaaacacga acggcgttag cataacgagg ggagtagtta aaagctctca ttgagcacct
     1981 ccgcatacaa aagcagaatg caagacgcgg cacgtttgtg catagaaaga atggtaatgc
     2041 ctgcacagca caaaatgcag atttttagga tcatgctcaa tcatggagac atctccgttt
     2101 taaaagcgaa aattgtcacc catatagcaa aagttggctg gaaacggcgt caatattgaa
     2161 gagaaatgta taaatattgg tgtagacgcg aacgcgtttt tcaccataat atgcccccaa
     2221 agacatgacg tatgtcttaa ccaaacccat gcaatatagc tgcggtacag ggactttgaa
     2281 gcgagcttct tggcggttgc gtagcttcaa agtccctttt gttttgtccc tataagggaa
     2341 cttttcgagt gtaccataac ctagctagag cctgttactc tgcatcgtta aaaaaataca
     2401 agaatgccat ttaagcgccc taaatagcgc tgtattgagt tttcatctac cttaatggta
     2461 aaccatgaac catcaaacag tcgcttctta tagcagcatt catgtgcttt tttttagatg
     2521 ttctaaacga gtctcaaacc ctgaaaaact tgcattggca tacttaccaa cggaagctat
     2581 ttaccataac atccgttgcc cgcaccatct cagactccct cagggcattt ctgcgtcctc
     2641 ctgcctattt ccggcatctt tccctgtcca tatcgtgaaa aaacgcgcct gatgatttct
     2701 tcgcgtctgc atggctatgc acacgaacgc atggacgtca ttttagcggc taaaatccgg
     2761 cgagcgccct gaaagccacc acgaacccca ccgataacgg tgaaagggtg cgcagtttac
     2821 tgtgcgaccg tgaggcgttg gccaggccgt caggcatggc aggatttatt gctaaggggg
     2881 tcggatttaa cctggattaa tcagcacaca gtgatgaacg ggattcggaa ggggcaccct
     2941 tgcggcgcgg cgatgtatac atcgctaacg tgccttggtc atttattgca agaagggaga
     3001 ggtagcattt tgatagcaaa aaagtagcaa aaggatagca accgaaagac cagaagactc
     3061 tggagatagc aaaaaagtag caaaatggta gcaaaaagat agcaaacatg cgggagctaa
     3121 ccaggagtct ttagcgggcc ggcggtactc caaaaacaca tcttgtcccg ctgttttgtg
     3181 aataagccag catagctgtg ggcacaaaac atttcctgtc cgctgcttta acctttgtaa
     3241 tccaggaacg tcaggtgacg tcactttgcg gcgttcgccc tatcctgcat cgtattaaaa
     3301 aatgcagcta tggcgcgtac ggtgccaaag agaagcccgc agatattccg cttcctcgct
     3361 cactgactcg ctccgctcgg tcgttcggct gcggcgagcg gagggaacag agacattaag
     3421 ccataggcaa tgagagagaa tggagaagca acaagaaagg ccgcggcgtt gccggttttc
     3481 cataggctcc gcccccctga caagcatcac agaatctgac gctcaaatca gtggtggcga
     3541 aacccgacag gactataaag atcccaggcg tttccccctg gtagctccct cgtgcgctct
     3601 cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg tttgtctcat
     3661 tccacgcctg acactcagtt ccgggtaggc agttcgctcc aagctggact gtatgcacga
     3721 accccccgtt cagtccgacc gctgcgcctg ccccggtaac tatcgtcttg agtccaaccc
     3781 gagaaggcac gacaaagcgc cactggcagc agccactggt aactgattta gaggagttag
     3841 tcttgaagtt gtgcgcctgt taaggctaaa ctgaaagaac aagttttggt gactgagctc
     3901 ctccaagcca gttacctcgg ttcaaagagt tggtagctca gagaaccttg aaaaaccacc
     3961 gttggtagcg gtggtttttt cttttgcaag acaagattaa gctctgtgta acatcagagt
     4021 caatagatct tcataccgat gttggtggcg tcggaggaat cggtggcaga agcggattaa
     4081 ctggcgtccc agatggaacg gctacttgag cttcagcatc tgtttttgtg ttactaatga
     4141 aaatatccgc cacacgattt tcaaaatcca cataactatc aataaactgt atatcaatat
     4201 gattcttata cttcgttgaa tatttctccg cattaccttt actatccttc ctttcatctg
     4261 tagaaaagaa gaacttatga tatctccttg gtgaaacccc ttcaaaggac tcaaataaaa
     4321 ccttaccact ttcgttaact tcagttattg catcatcgtg caactctaaa gctaagcttt
     4381 tttcataagg ttcaatgtaa acaactttat tgataccaac agcaacaata tgtctcgcac
     4441 aattatgaca ggggaacgta gttgtatata aaaccttatc ttcaaaacca ccattcccga
     4501 gtctagcaag agatgtaata gcatccattt cagcatgaat agagcgagag tactcaatga
     4561 ttgatgatac aggagtacct tcgtagatta ttccagcaac cttttctgct tgttcttgag
     4621 ttaact
//
