LOCUS       NZ_CP054834             8128 bp    DNA     circular CON 12-SEP-2023
DEFINITION  Staphylococcus saprophyticus strain UTI-045 plasmid pUTI-045-3,
            complete sequence.
ACCESSION   NZ_CP054834
VERSION     NZ_CP054834.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15089826
            Assembly: GCF_013358365.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus saprophyticus
  ORGANISM  Staphylococcus saprophyticus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8128)
  AUTHORS   Stephens,C., Wright,M., Hartman,A., Gonzales,A., Sensabaugh,G.,
            Robinson,P., Lopez,G. and Hess,D.
  TITLE     Complete genome sequences of diverse uropathogenic Staphylococcus
            saprophyticus isolates from a college health center
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8128)
  AUTHORS   Stephens,C., Wright,M., Hartman,A., Gonzales,A., Sensabaugh,G.,
            Robinson,P., Lopez,G. and Hess,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUN-2020) Biology, Santa Clara University, 500 El
            Camino Real, Santa Clara, CA 95054, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 173x
            Sequencing Technology :: Illumina MiSeq, Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/12/2023 03:28:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,741
            CDSs (total)                      :: 2,657
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 36 of 87
            Pseudo Genes (internal stop)      :: 27 of 87
            Pseudo Genes (multiple problems)  :: 14 of 87
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP054834.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8128
                     /organism="Staphylococcus saprophyticus"
                     /mol_type="genomic DNA"
                     /strain="UTI-045"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:29385"
                     /plasmid="pUTI-045-3"
                     /geo_loc_name="USA: California"
                     /collection_date="2019"
     gene            join(7306..8128,1..137)
                     /locus_tag="HSZ48_RS13505"
                     /old_locus_tag="HSZ48_13550"
     CDS             join(7306..8128,1..137)
                     /locus_tag="HSZ48_RS13505"
                     /old_locus_tag="HSZ48_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002508766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_069794587.1"
                     /translation="MATTKLGNTKSASRAINYAEKRAEEKSGLNCDVDYAKSAFKATRS
                     LYGKEDGIQAHTIIQSFKPGEVTPEQCNQIGLELAEKIAPNHQVAVYTHTDKNHYHNHI
                     VINSIDLETGNKYQSNKKQREFVKQANDNICREYGLSVPEKQRETRYTQAEYEIGKEAQ
                     KGNKPIPWKEQIRMVIDETQAINYEQLGADLLQNGIKIERITEKTITYKHLEEDKKVRG
                     SKLGEKYDKGGLERGYDGRIKSREQENTRETGPDRKATQSEWDNFADNTRKLEHDRRSS
                     EAARLAHEKSIRDKEEREREREQAQRTSKKSRGFDLEL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            169..654
                     /locus_tag="HSZ48_RS13510"
                     /old_locus_tag="HSZ48_13555"
     CDS             169..654
                     /locus_tag="HSZ48_RS13510"
                     /old_locus_tag="HSZ48_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002508774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF334 domain-containing protein"
                     /protein_id="WP_081332184.1"
                     /translation="MNVARSYLRKLDTDNQEQDFKQAFDEKFKDLDKQASDLFEQYKKH
                     NDQARKEMTEYKKTITNRLDKNDTIVENLNKSLDIMTKGVVSLFFVVAIIALVSLVTGP
                     ISNFFGISQGYDFINHEIATKESIWRYLWGVLYVLPYVFFGFLIHGVLKAFNAIRWK"
                     /name="DUF334 domain-containing protein"
     gene            1542..2408
                     /locus_tag="HSZ48_RS13515"
                     /old_locus_tag="HSZ48_13560"
     CDS             1542..2408
                     /locus_tag="HSZ48_RS13515"
                     /old_locus_tag="HSZ48_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002512613.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="WP_069794589.1"
                     /translation="MTGETVVYRNEMNLVPLRKFTSTEVDLFFTLCNKLKEQDTRRLEI
                     YFDELKHLSNYYSRSQDRFVKDLEHVYDKMLNLTYTERNGKSFKKFVLFTSYEVDVEEQ
                     QLAISINSDLKHILNSITADFTKFELQEMTYLKSTYAKNMFRLLKQYKSTGYLKISVED
                     FRERLDVPKSYRMTDLNKNIFTPIINELSSIFKGLNINKVKAKKGRKIEWLEFTFEPEK
                     RIHSKRQPEMANIGKKTQNINREMTPEWLENKKYEPIKPKTTEFTEQQKEAFLEKMNIN
                     GNYRANK"
                     /name="RepB family plasmid replication initiator protein"
     gene            2427..2936
                     /locus_tag="HSZ48_RS13520"
                     /old_locus_tag="HSZ48_13565"
     CDS             2427..2936
                     /locus_tag="HSZ48_RS13520"
                     /old_locus_tag="HSZ48_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002487969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated family protein"
                     /protein_id="WP_069794592.1"
                     /translation="MKTVKEVSESLNVSKQTIHYHLKNLPSNLKVKKTGNKSLIDDSVI
                     AYLESILNKKSTKESTNKSSSFDEKSTKESTNKKEPMKARHDSYIDKYIAHLESEIRQK
                     NRQIDDLTIALKQEQSLNLNSQNILSHNDPTETDDLNTKEEIFETKTPDQSEQKQSIFS
                     KLFKKK"
                     /name="replication-associated family protein"
     gene            3656..4354
                     /locus_tag="HSZ48_RS13525"
                     /old_locus_tag="HSZ48_13570"
     CDS             3656..4354
                     /locus_tag="HSZ48_RS13525"
                     /old_locus_tag="HSZ48_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175396.1"
                     /GO_component="GO:0009986 - cell surface [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing protein"
                     /protein_id="WP_069794580.1"
                     /translation="MKKRTLFSTLLLASSLTFTGFSATVNAEDGAKTVYGENLPNSADT
                     NGDGWANVDFNTSSLPQSAENQISELSKQKDSGELTQIEYNEKVSSIFNEYQSQSADIQ
                     SDERTQQGERPFGGVIPNGMTQEEYQELESNVPNPNSVSTEKYNQIVENESQKIANEEN
                     KTINTPQGEFTPVTENTNKNSEEVKSKDSNSQVLPETGESNQSYILSIIGASGLLILGI
                     GLLLFRKKLT"
                     /name="LPXTG cell wall anchor domain-containing protein"
     gene            4529..4873
                     /locus_tag="HSZ48_RS13530"
                     /old_locus_tag="HSZ48_13575"
     CDS             4529..4873
                     /locus_tag="HSZ48_RS13530"
                     /old_locus_tag="HSZ48_13575"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_081324949.1"
                     /translation="MKKIILPILIVSLLVILVSCNNNDSENHDNNSSSDTNTSQSKDNE
                     KNKIDEETNISGNGDNAEGNNKQNKEQNDNKNQEKNNDSNENQQSTDKNKSYLNNFNSK
                     EFVFHNSNSP"
                     /name="hypothetical protein"
     gene            4962..5053
                     /locus_tag="HSZ48_RS13535"
                     /old_locus_tag="HSZ48_13580"
     tRNA            4962..5053
                     /locus_tag="HSZ48_RS13535"
                     /old_locus_tag="HSZ48_13580"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /name="tRNA-OTHER"
     gene            5152..6390
                     /locus_tag="HSZ48_RS13540"
                     /old_locus_tag="HSZ48_13585"
     CDS             5152..6390
                     /locus_tag="HSZ48_RS13540"
                     /old_locus_tag="HSZ48_13585"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002508762.1"
                     /GO_component="GO:0005737 - cytoplasm [Evidence IEA]"
                     /GO_function="GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA]"
                     /GO_process="GO:0071555 - cell wall organization [Evidence
                     IEA]; GO:0009252 - peptidoglycan biosynthetic process
                     [Evidence IEA]; GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="WP_069794582.1"
                     /translation="MYFTKLDVNEFQSFTNSYFSHYTQTQNHYIYRKQKQNDAHLVGVK
                     DNENNILAACLLTEARSLKFFKYFYTHRGPVLDFNNYELVRFFYKHLTKYLKKHRGLFV
                     LTDPYTLENIRNSQGEILESYNNQPLIKTMEDLGYKHQGYTVGYSQMSQIRWLSVLNLN
                     NRTEDQLLKDMDYQTRRNIKKTHEMGVKVCTLPIEETDRFFKLFKMAEEKHGFKFRDKK
                     YFEEMQKMYQTNSMLKLAYIDLSELLMKQKKNLENLTSTLENTKIDLESNPDSKKSKNK
                     YEQELQQIEAQKRKISETENLIKTDGTILDLAAAFYIFNNHEVYYLSSGSNPKYNKYMG
                     AYKLQWEMIKFANEQNITRYNFYGITGDFSENAEDFGVQKFKSGFNAHVEEYIGDFIKP
                     IRPLLYKIYNIIK"
                     /name="aminoacyltransferase"
     gene            6754..7137
                     /gene="mobC"
                     /locus_tag="HSZ48_RS13545"
                     /old_locus_tag="HSZ48_13590"
     CDS             6754..7137
                     /gene="mobC"
                     /locus_tag="HSZ48_RS13545"
                     /old_locus_tag="HSZ48_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002449121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_069794585.1"
                     /translation="MSEHDNNLASDLSVGENRKPNRKEPKQISFRVSESEYEKLRSSAE
                     TLNMSVPNFVKKKAHGSRLVAPKFDKETRQSIAKDLSKLGANVNQIAKYCNQHQHEAPN
                     YEGLEYNINAVRERLDEIWQKIN"
                     /name="mobC"
     gene            7119..7319
                     /locus_tag="HSZ48_RS13550"
                     /old_locus_tag="HSZ48_13595"
     CDS             7119..7319
                     /locus_tag="HSZ48_RS13550"
                     /old_locus_tag="HSZ48_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019468020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_069794596.1"
                     /translation="MAKDKLRIIFNIILVVLVLGYGAFVLFTDYGNAKWSLIFIGILIV
                     VGVLRDIITPKPTSSDENGNN"
                     /name="hypothetical protein"
     Typing          7307..8127
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.891"
                     /coverage="86.0"
                     /evalue="3.96e-175"
ORIGIN
        1 aacacgatag aagaagttct gaagcagcaa gacttgctca tgagaaatct atccgagata
       61 aagaagaacg agaaagagag agagaacagg cacaacgaac ttctaaaaag tctagaggat
      121 tcgacctcga actttgatga atcaacacat gctacaaaag ataattttat gaatgtggca
      181 agaagttatt taagaaaatt agatacagat aatcaagaac aagattttaa acaagccttt
      241 gatgaaaaat ttaaagacct agataaacaa gcaagtgatt tatttgagca atataaaaaa
      301 cataatgacc aagcgagaaa agaaatgacg gaatacaaaa agacgattac gaatcgctta
      361 gataaaaacg atacaatcgt cgaaaattta aataaatcct tagatattat gacgaaaggt
      421 gttgtgtctt tattttttgt tgtcgcaatc attgctttgg tttcgttggt aacgggacca
      481 attagtaact tttttggcat ttcacaaggc tacgacttta ttaatcatga aatagcgaca
      541 aaagaaagta tttggagata tttatggggt gttttatatg ttttaccgta tgtttttttc
      601 ggttttctta tacatggtgt tttaaaagca tttaacgcca taagatggaa atgagttata
      661 aaacatcata aacaatagtt atacttctaa atagcccctt aaaattcaaa ataaaggcaa
      721 ttaaaaagcc atctcaatat aaaattgaaa tggctgaaat aaaggatatg cgttcttagt
      781 taataatatc tataaaaaag actacgtgaa tttcattcaa atttcaaatt aaaaactatt
      841 attagcagga aaactaatag tagtcagtgt gaattctcaa gagttaagta gagatagtgc
      901 taaatgtact ataccttaag ctctttttga caatgttata ggtgttaatt ttgttgtgtg
      961 tcaacggtaa cggaatgagc gtagcgatat gagtataaga agttgataca cttgcactta
     1021 ctcgccacac taaactcagt ggaattggac aacctccaat tccctgcagt ttgaacggcg
     1081 agttcaagaa gttttttgat tttacttttt ttcaaaaaag taagggttta ggtttttaaa
     1141 atagatataa attccttcag aatttattca tttttgaagt ggttttgaat tagattttga
     1201 aatggggcaa aattgccccg aactttatat aatcttttta agaacttttt aaaccctttt
     1261 taggtgccct tttaacctta ctgcgagaag ggttttcaga ccctaaagga gtagttttgt
     1321 aacagtaaag gggtagtttt gtaacagtaa aggagtagtt ttgtaacagt aaaggagtag
     1381 ttttgtaaca gtaaaggagt agttttaaaa aagtcgctcc tttaatctct tgacattgta
     1441 ttatcagagg tttagagtga ctttaagaaa taaaaaagga gtacaactgg gaattgtact
     1501 ccttttcaaa tttattttat tctactagga gtgattataa catgactgga gaaactgttg
     1561 tgtatcgaaa cgaaatgaat ttagtacctt taagaaaatt tacaagtaca gaagtagatt
     1621 tgttttttac tctatgtaat aagctcaaag aacaggacac acgtagacta gaaatatatt
     1681 ttgatgaatt aaaacatctt agtaattact attctcgctc acaagatcgt tttgtgaaag
     1741 atttagagca tgtatacgat aaaatgttga atttaactta tacagaacgt aatggaaaat
     1801 catttaaaaa gtttgtttta tttacatcgt acgaagtgga tgttgaagaa caacagctag
     1861 caattagtat taatagtgat ttgaagcata tactcaactc tattacagct gattttacta
     1921 aatttgaact acaagaaatg acttatctca aatcaacata cgctaaaaac atgtttcgac
     1981 tactgaaaca atataaaagt actggttatt taaaaattag tgttgaagat tttagagaac
     2041 gtttagatgt tcctaaatct tatcgtatga ccgatttaaa taaaaatatt tttacgccaa
     2101 taattaatga attaagctca atttttaagg ggctaaatat aaataaagtt aaagctaaaa
     2161 aaggtcgtaa aattgagtgg ttagaattca cttttgagcc tgagaaacgc attcattcaa
     2221 agcgacaacc tgaaatggcg aatataggta agaaaacaca aaatataaat cgtgagatga
     2281 cgccagaatg gttagaaaat aaaaagtatg aacctatcaa acccaaaaca actgaattta
     2341 cagaacaaca aaaagaagcg tttttagaaa aaatgaatat aaatggaaac tatagggcaa
     2401 acaaataaag taggacgtgt tatttgatga aaacggtaaa agaagtttct gaaagtttaa
     2461 atgtttctaa acaaacaatt cactatcact taaaaaattt accatcaaat ttaaaagtta
     2521 aaaaaacagg caataaatcg ttaatagatg atagtgtaat agcttattta gagtcgattt
     2581 tgaataaaaa atcgacaaaa gaatcgacaa acaaatcgtc aagctttgac gaaaaatcga
     2641 caaaagaatc gacaaacaaa aaagaaccta tgaaggcgcg tcacgacagt tatattgata
     2701 aatatatcgc acacttagaa tcagaaattc gacaaaagaa tcgacaaatt gacgatttaa
     2761 caattgcctt aaaacaagaa caatctttga acttaaatag ccaaaatata ttaagtcata
     2821 acgaccctac agaaactgac gatttaaata caaaagaaga gattttcgag acaaaaacac
     2881 cagatcaaag tgaacaaaaa cagtctattt tctcaaaatt attcaaaaaa aagtaacata
     2941 gatttgagta aactgcagtt aaaaaataag aatggctatt cttattaaaa aagtgaatta
     3001 taaatccctt catgacagca ttcttcttta tcatatattg gttgtgtaat aaggaggaat
     3061 gtctattatc cttgtcatac cagcatatat agagtgagtt cggaaaagcg aactaagttt
     3121 ctttttggaa aatattatgt tattatacaa ttaacgaatg agttacaact tagtatatac
     3181 agaaagcccc cacacgccgg caagctaatg tgggggcttt tattttatcc cttaagttga
     3241 tcaaatatat agttcgcaac tataccggcc acaactgatg agataaaacc taagacaaat
     3301 gagattacaa caagcatata cagcaccccc tttctgcaca ggaagtgctt ttctagtata
     3361 acaaaaatgt ataagttcat acggtacttt aggaatttgt ataaaaatac aaaagtaaga
     3421 tgtatgaaaa cacatgaaaa cgtgttaaac cgacaatgta taaagtgtgt ataaaagttc
     3481 acaatccata aaagccaaaa acactgatct aacagtgttt cggcctttgt gtttagttaa
     3541 cataataaag agttataggt agcgaaaatg tataatatat acagtttttc atttttattt
     3601 ttttttatgt taagctaaaa ttgaacaaaa acataaatat tgggaggaat gttttatgaa
     3661 aaaacgcaca ttattttcaa ctttattatt ggctagctca ttaacattta caggtttttc
     3721 agcgacagtc aatgctgagg atggtgccaa aacagtatac ggagagaatt taccaaatag
     3781 tgcagataca aatggagatg gttgggctaa tgtagacttc aatacttcat cccttccaca
     3841 atctgcagaa aatcagataa gtgaactttc taaacagaaa gattcaggag aattaactca
     3901 aattgagtat aatgagaagg tttcaagtat atttaatgaa tatcaatcac aaagtgctga
     3961 tattcaaagt gatgagagaa ctcaacaagg agaaagacct ttcgggggtg tgatacctaa
     4021 tggtatgact caagaagaat accaagagtt agaaagcaat gttcctaacc ctaatagtgt
     4081 atccacagaa aaatataatc aaattgtaga aaatgaaagc caaaagatag ctaatgaaga
     4141 aaataaaact atcaatacac ctcaaggtga gtttacacct gtaactgaaa atacaaataa
     4201 gaattctgaa gaagtgaaaa gtaaagattc aaattctcaa gtattaccag agacaggcga
     4261 aagcaaccaa agttatatat tgtcaataat tggcgcttct ggactactaa tattaggtat
     4321 cggtctttta ctattcagaa aaaaattaac ataaaaattg ataaaataac cacctgtttt
     4381 acataaacac ggtggttatt ttattgtata tttaatacat atcatgttat agatgttata
     4441 cagcagtttt aacctctgta atttaagttt tcttattgga aaaatatcaa cataggttta
     4501 atttgaaatg ttgaaaaggg gttataatat gaaaaaaatc attttaccta tattaattgt
     4561 tagtttattg gtaattttag tgagttgtaa taataacgat tcagaaaatc acgataataa
     4621 tagttctagt gatactaata cctcacaaag taaagataat gaaaagaata aaattgatga
     4681 ggaaactaac atatctggta atggtgataa tgcagaaggt aacaataaac aaaataaaga
     4741 acaaaatgat aataaaaacc aagaaaaaaa taatgatagt aatgaaaatc aacaatctac
     4801 tgataaaaat aaaagctact taaataattt taactcaaaa gaatttgttt tccacaattc
     4861 taattccccc taatttaatt ttacaaatcc agattaacat atattttata tactttacat
     4921 ttatattaaa aaaagataag atagataggc agttaaattt tggagggata cccaagtctg
     4981 gcttaaggga aattaatatt tattattaat taggagcttt gcgcttgcgt aggttcgaat
     5041 ccttctccct ccgttaaatt tcattttaag ccaagccttt ttaaagtgct tggctttttt
     5101 atggtcttta ttatatagaa acgcaatatt ctattagaaa gtaggttaat aatgtatttt
     5161 accaagttag atgttaacga atttcaaagt tttactaata gttatttttc tcactataca
     5221 caaactcaaa atcattatat ttatagaaaa caaaaacaaa atgatgctca tttagttgga
     5281 gttaaagata acgaaaataa tatattagca gcatgtcttt taactgaagc acgatcttta
     5341 aagtttttta aatattttta cactcatcgt ggacctgttt tagactttaa taattatgaa
     5401 ctagtaagat ttttctataa acacttaact aaatacttaa aaaaacatcg tggtttattt
     5461 gttttaactg atccttatac tctagaaaac ataagaaatt ctcaaggaga aatattagaa
     5521 agttataata atcaaccttt aattaaaact atggaagatt taggctataa acatcaagga
     5581 tatactgttg gctattctca aatgagccaa atccgttggt tatctgttct taatttaaat
     5641 aatagaacag aagatcagtt gcttaaagat atggattatc aaactagaag aaacataaaa
     5701 aaaacacatg aaatgggtgt gaaagtatgt acattaccaa ttgaagaaac agatagattt
     5761 ttcaaattat ttaaaatggc tgaagaaaag catggtttca aatttagaga taaaaaatac
     5821 tttgaagaaa tgcaaaaaat gtatcaaact aatagtatgt taaaattagc gtatattgat
     5881 ttatcagaac ttctaatgaa gcagaagaaa aacctagaaa atttaactag tacactagaa
     5941 aatactaaaa tagacttaga gtctaatcca gattctaaaa aaagtaagaa taagtatgaa
     6001 caagagttac aacaaataga agcacaaaag agaaaaatat ctgaaacaga aaatttaatt
     6061 aaaacggatg gaacaatatt agatttagct gctgcttttt atatttttaa taaccatgaa
     6121 gtttattatc tttctagtgg atcaaatccc aagtacaaca aatatatggg cgcttataaa
     6181 ttacaatggg aaatgataaa atttgcgaat gaacagaata ttacacgcta taatttctat
     6241 ggtataacag gtgatttcag tgaaaatgct gaagattttg gcgttcaaaa atttaaatct
     6301 ggattcaatg ctcatgttga agaatatata ggagatttca ttaagcccat tcgtccttta
     6361 ttatataaaa tttataatat aataaaataa tgtatgataa ttctaaaaga acgaatatga
     6421 tatatactat ttaactatga agttttcatt tattatttaa ccaaaaatat tttttgaatt
     6481 tagcaatttc taccaaccat atttttggtt gcagagtttt tgattttgaa tttggttttg
     6541 aactatgagt ggctagcatt ttaccactca ttttttgcgt acgcaaaaac ataaagggta
     6601 tgggaatatt cccatcaagc cggtatattc agaacgaagt ggctaggata tacgacgctt
     6661 gccaaaccac attataactt gaaattttgt tgattatcct ataatctgca aatataaaaa
     6721 gttataaatt tcgaagaaac gtggtgggat tgaatgagtg aacatgataa taatttggct
     6781 agcgatttgt ctgttgggga aaaccgaaaa cccaaccgca aagagccaaa acaaattagt
     6841 ttcagagtga gcgaatccga atatgaaaag ttaaggtcat ctgccgaaac tttgaatatg
     6901 agtgtgccga attttgttaa gaaaaaggca catggtagtc gattggtagc gcccaaattt
     6961 gataaagaga cgcgacaatc gattgccaaa gatttaagta agttgggtgc gaatgtaaat
     7021 cagattgcta aatattgcaa tcaacatcaa catgaagccc cgaattatga aggcttggaa
     7081 tataatatca atgcagtacg tgaaaggttg gatgagatat ggcaaaagat aaattaagaa
     7141 ttatatttaa cattatttta gttgttttgg ttttaggata tggtgctttt gtattattca
     7201 cagattacgg aaatgcaaaa tggtcactta tttttattgg tattttaatt gttgttggag
     7261 ttttaagaga tatcattaca cctaaaccaa caagtagtga tgaaaatggc aacaactaaa
     7321 ttaggaaata caaaatcggc aagcagggca atcaattacg cagaaaagcg agcagaagaa
     7381 aaaagtggtt tgaattgcga tgtagattac gccaaatcag ctttcaaagc cacacggtct
     7441 ttatatggca aagaagatgg gattcaagca catacgatta tccaatcatt caagcctggc
     7501 gaagtaacac ccgaacaatg taatcaaatc ggtttagaat tagccgagaa aatcgcaccc
     7561 aatcatcaag tcgccgttta tacgcataca gataaaaatc attaccacaa ccatattgtg
     7621 attaattcaa ttgaccttga gacaggtaat aaatatcaaa gtaataaaaa gcaacgtgaa
     7681 ttcgttaaac aagccaatga caatatttgt cgtgaatatg gtttatcagt tccagaaaaa
     7741 caaagagaaa cacgctacac acaagccgaa tatgaaattg gaaaagaagc tcagaaaggg
     7801 aataaaccga taccttggaa agaacaaatt cgtatggtca tagatgaaac acaagcgatc
     7861 aattatgaac agttaggcgc tgatttgcta cagaacggca taaaaattga acgtatcact
     7921 gaaaaaacga ttacttacaa acatcttgaa gaagataaaa aagtacgggg cagtaaacta
     7981 ggtgaaaaat acgataaagg gggcttagaa cgtgggtatg atggacgaat taaatccaga
     8041 gaacaagaaa atactagaga aactggacca gatagaaaag caactcaatc agagtgggac
     8101 aacttcgcag acaacaccag aaaacttg
//
