LOCUS       NZ_CP054833            19933 bp    DNA     circular CON 12-SEP-2023
DEFINITION  Staphylococcus saprophyticus strain UTI-045 plasmid pUTI-045-2,
            complete sequence.
ACCESSION   NZ_CP054833
VERSION     NZ_CP054833.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15089826
            Assembly: GCF_013358365.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus saprophyticus
  ORGANISM  Staphylococcus saprophyticus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 19933)
  AUTHORS   Stephens,C., Wright,M., Hartman,A., Gonzales,A., Sensabaugh,G.,
            Robinson,P., Lopez,G. and Hess,D.
  TITLE     Complete genome sequences of diverse uropathogenic Staphylococcus
            saprophyticus isolates from a college health center
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 19933)
  AUTHORS   Stephens,C., Wright,M., Hartman,A., Gonzales,A., Sensabaugh,G.,
            Robinson,P., Lopez,G. and Hess,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUN-2020) Biology, Santa Clara University, 500 El
            Camino Real, Santa Clara, CA 95054, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 173x
            Sequencing Technology :: Illumina MiSeq, Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/12/2023 03:28:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,741
            CDSs (total)                      :: 2,657
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 36 of 87
            Pseudo Genes (internal stop)      :: 27 of 87
            Pseudo Genes (multiple problems)  :: 14 of 87
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP054833.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..19933
                     /organism="Staphylococcus saprophyticus"
                     /mol_type="genomic DNA"
                     /strain="UTI-045"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:29385"
                     /plasmid="pUTI-045-2"
                     /geo_loc_name="USA: California"
                     /collection_date="2019"
     gene            join(19697..19933,1..1407)
                     /gene="merA"
                     /locus_tag="HSZ48_RS13390"
                     /old_locus_tag="HSZ48_13435"
     CDS             join(19697..19933,1..1407)
                     /gene="merA"
                     /locus_tag="HSZ48_RS13390"
                     /old_locus_tag="HSZ48_13435"
                     /EC_number="1.16.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031939.1"
                     /GO_function="GO:0016152 - mercury (II) reductase activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercury(II) reductase"
                     /protein_id="WP_175268021.1"
                     /translation="MTQNSYKIPIQGMTCTGCEEHVTEALEQAGAKDVSADFRRGEAIF
                     ELSDDQIEKAKQNISAAGYQPGEEESQPSENSVDFNRDGDYDLLIIGSGGAAFSAAIKA
                     NENGAKVAMVERGTVGGTCVNIGCVPSKTMLRAGEINGLAQNNPFTGLQTSTGAADLAQ
                     LTEQKDGLVSQMRKEKYIDLIEEYGFDLIRGEASFIDDKTIQVNGQNITSKSFLIATGA
                     SPAVPEIPGMNEVDYLTSTSALELKEVPQRLAVIGSGYIAAELGQMFHNLGTEVTLMQR
                     SERLFKTYDPEISEAIDESLTEQGLNLITGVTYQKVEQNGKSTSIYIEVNGQEQVIEAD
                     QVLVATGRKPNTETLNLESAGVKTGKKGEVLTNEYLQTSNNRIYAAGDVTLGPQFVYVA
                     AYEGGIVANNALGLAKRKIDLRFVPGVTFTNPSIATVGLTEQQAKEKGYDVKTSVLPLD
                     AVPRALVNHETTGVYKLVVNAQTQKLIGAHIVSENAGDVIYAATLAVQFGLTIEDLTDS
                     FAPYLTMAEGLKLAALTFDKDVSKLSCCAG"
                     /name="merA"
     gene            1489..2139
                     /gene="merB"
                     /locus_tag="HSZ48_RS13395"
                     /old_locus_tag="HSZ48_13440"
     ARG             1490..2136
                     /name="merB"
                     /gene="merB"
                     /gene_name="organomercurial lyase MerB"
                     /tool="amrfinderplus"
                     /drug_class="MERCURY"
                     /identity="98.61"
                     /coverage="100.0"
     gene            complement(2334..>2633)
                     /locus_tag="HSZ48_RS13400"
                     /old_locus_tag="HSZ48_13445"
                     /pseudo=""
     CDS             complement(2334..>2633)
                     /locus_tag="HSZ48_RS13400"
                     /old_locus_tag="HSZ48_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377078.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(2693..3367)
                     /locus_tag="HSZ48_RS13405"
                     /old_locus_tag="HSZ48_13450"
     CDS             complement(2693..3367)
                     /locus_tag="HSZ48_RS13405"
                     /old_locus_tag="HSZ48_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS257 family transposase"
                     /protein_id="WP_001105987.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYALSYRDISEILRERGVNVHHS
                     TVYRWVQEYAPILYQIWKKKHKKAYYKWRIDETYIKIKGKWSYLYRAIDAEGHTLDIWL
                     RKQRDNHSAYAFIKRLIKQFGKPQKVITDQAPSTKVAMAKVIKAFKLKPDCHCTSKYLN
                     NLIEQDHRHIKVRKTRYQSINTAKNTLKGIECIYALYKKNRRSLQIYGFSPCHEISIML
                     AS"
                     /name="IS6-like element IS257 family transposase"
     gene            complement(3950..4558)
                     /locus_tag="HSZ48_RS13410"
                     /old_locus_tag="HSZ48_13455"
     CDS             complement(3950..4558)
                     /locus_tag="HSZ48_RS13410"
                     /old_locus_tag="HSZ48_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002474535.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_002380765.1"
                     /translation="MIIGYARVSSIDQNLERQLDNLKTFGVEKIFTEKQSGKSVENRPV
                     FQEALNFVRMGDRFVVESIDRLGRNYDEVINTVNYLKDKEVQLMITSLPMMNEVIGNPL
                     LDKFMKDLIIQILAMVSEQERNESKRRQAQGIKVAKEKGVYKGRPLLYSPNAKDPQKRI
                     IYHRVVEMLERGESITKISKEVNITRQTVYRIKHDNNLT"
                     /name="recombinase family protein"
     gene            4846..5511
                     /locus_tag="HSZ48_RS13415"
                     /old_locus_tag="HSZ48_13460"
     CDS             4846..5511
                     /locus_tag="HSZ48_RS13415"
                     /old_locus_tag="HSZ48_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020365870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5079 family protein"
                     /protein_id="WP_012816722.1"
                     /translation="MEVNLQKKSIESIQHSHLIGLSLVNFIIFAVYYFIYAGPGIKGNM
                     PLYMKVYLIGSLLLWLLLYLQEREYIFKHYNRKNYKFRVVIYMTINLLSGYNIPFLISS
                     AYAFYFADSRDDTFTYWLLICGLIIVSAIGLFLFCLGEFEMFGIQNGSFIRMIGILTIL
                     LSFGLLLYVSLILPVDSEENRTIWMGMIMLFCSHLLIGRTYFYLGLLNYDIKEDGMKL"
                     /name="DUF5079 family protein"
     gene            5695..5820
                     /locus_tag="HSZ48_RS13835"
     CDS             5695..5820
                     /locus_tag="HSZ48_RS13835"
                     /inference="COORDINATES: protein motif:HMM:NF028205.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_258534050.1"
                     /translation="MFISLNDRLQHISELNIEYIDMLKVDPTLSKKCFNSERYKN"
                     /name="hypothetical protein"
     gene            5870..6457
                     /locus_tag="HSZ48_RS13425"
                     /old_locus_tag="HSZ48_13470"
     CDS             5870..6457
                     /locus_tag="HSZ48_RS13425"
                     /old_locus_tag="HSZ48_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002446083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5080 family protein"
                     /protein_id="WP_012816724.1"
                     /translation="MVYFVLIGVFIIFYLALFDSLLKSENFSILSIFLDFVIVIMLIIF
                     HFIGNKLTGNDLSNYIAFTLIGSFVYMYFAIKNFWTKPKVLNYLISKKENTNQDDIEYQ
                     ELDLQVSKIKSVYYFLIAIALLILTKVHIVSDIKADSLSMNPVFIFVGIIIIVIWLIMD
                     LYRKKKYGIFLFKSLIPIVVTIWIIMSSVLLY"
                     /name="DUF5080 family protein"
     gene            complement(6728..6823)
                     /locus_tag="HSZ48_RS13430"
                     /old_locus_tag="HSZ48_13475"
     CDS             complement(6728..6823)
                     /locus_tag="HSZ48_RS13430"
                     /old_locus_tag="HSZ48_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_011276848.1"
                     /translation="MLEILVHITTTVISGCIIALFTHWLRNRKDK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            7174..7473
                     /locus_tag="HSZ48_RS13435"
                     /old_locus_tag="HSZ48_13480"
     CDS             7174..7473
                     /locus_tag="HSZ48_RS13435"
                     /old_locus_tag="HSZ48_13480"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_225309949.1"
                     /translation="MPKEKITREELGKQMMETTIIKSNIKKVKNPSNDQYESLRSRTSI
                     KIHRDFKPLFDELVAEERENKIDFVDALLVFYAQEKYPEILKAFKKQELKTQKK"
                     /name="hypothetical protein"
     gene            <8851..9118
                     /locus_tag="HSZ48_RS13840"
                     /pseudo=""
     CDS             <8851..9118
                     /locus_tag="HSZ48_RS13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001110055.1"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]; GO:0008483 - transaminase activity
                     [Evidence IEA]"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /name="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            9177..10166
                     /gene="bioB"
                     /locus_tag="HSZ48_RS13440"
                     /old_locus_tag="HSZ48_13485"
     CDS             9177..10166
                     /gene="bioB"
                     /locus_tag="HSZ48_RS13440"
                     /old_locus_tag="HSZ48_13485"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017723563.1"
                     /GO_function="GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]; GO:0051539 - 4 iron, 4 sulfur cluster
                     binding [Evidence IEA]; GO:0004076 - biotin synthase
                     activity [Evidence IEA]; GO:1904047 -
                     S-adenosyl-L-methionine binding [Evidence IEA]"
                     /GO_process="GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="WP_041080315.1"
                     /translation="MNLAEKILKGETLTKERLLKIYENPNIDTLDLLNEAYILRKHYYG
                     KKVKLNMILNAKSGICPENCGYCGQSRDMKQKQRYALVPEEQIVSGAKVAHENNIGTYC
                     IVMSGRGPSNKEIDHISRTVEEIKSNHPQLKICACLGLTNDEQAKKLKSSGVDRYNHNI
                     NTSENYHEEVVTTHTYKDRTNTLEIMKENNISPCSGVICGMGESNQDIIDMAFALKEID
                     ADSIPINFLHPIKGTKFGEMDELTPMKCLRLISLFRIVNPSKEIRIAGGREVNLRSLQP
                     LALKAANSIFVGDYLITGGQPNKLDYKMIEDLGFEIDSGYNELKEKVE"
                     /name="bioB"
     gene            10398..10814
                     /locus_tag="HSZ48_RS13445"
                     /old_locus_tag="HSZ48_13490"
     CDS             10398..10814
                     /locus_tag="HSZ48_RS13445"
                     /old_locus_tag="HSZ48_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="WP_069821423.1"
                     /translation="MDFYQMPMFNKILVQDIEKAEKWYEKTLGFKSVFKFRNEKNEVLM
                     NHLRLEKYQDLMLISSNDFKVGNSIYLNLLVKDIESKSQTISPKFVISELEEQPWNAKE
                     MTIKDPEGHLITLTQSNISDTDFEKLMQQTSKNY"
                     /name="VOC family protein"
     gene            complement(11166..11666)
                     /locus_tag="HSZ48_RS13450"
                     /old_locus_tag="HSZ48_13495"
     CDS             complement(11166..11666)
                     /locus_tag="HSZ48_RS13450"
                     /old_locus_tag="HSZ48_13495"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002508662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="WP_175268022.1"
                     /translation="MEFYSRDLKNTDIDRLNELYALPEVTQYQTWGPQSYEETQQFINM
                     VLDKDSNWIYNVLVDPDTDKVIGTIQLAIDEVNQSAEINFIVHPNHWSNGIATDIAKTI
                     IKYAFKVLKLNRIWASIDSSNIAAGIVLEKLGMKREGILRQDKLVQDTYRDTLIYGFLR
                     SEY"
                     /name="GNAT family protein"
     gene            complement(11772..12743)
                     /locus_tag="HSZ48_RS13455"
                     /old_locus_tag="HSZ48_13500"
     CDS             complement(11772..12743)
                     /locus_tag="HSZ48_RS13455"
                     /old_locus_tag="HSZ48_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="WP_175268023.1"
                     /translation="MSRKNIKNQASQNFYMLHKVLFINEKYKKLSDSAKVTYAILNDRV
                     SLSIKNNWIDQNGDIYFIFTNENLQEVLDKSKNTITKIKKELQEVGLLEQVKTGFNRPN
                     KLYLHEIETNINIEKEIQNSTSNNDESSDSKDSHCLGVQNPKQWESRIPNNGSPESQNL
                     DPNDTDLSNTDYIETENNDTHDMNDTYNNPIKNTHSDHTNHQQTEFNNDALKFQVLEEL
                     PQQLQDYLSKFEIREIRIIKSVLLKGKKSFNNAHDTYYRLEDVEFEIVSVLKRFKAMLL
                     QKNETVEAMQGYLMQSIKAELEEIHALNMRRQNMKQHNIFNQ"
                     /name="replication initiator protein A"
     gene            13164..13898
                     /locus_tag="HSZ48_RS13460"
                     /old_locus_tag="HSZ48_13505"
     CDS             13164..13898
                     /locus_tag="HSZ48_RS13460"
                     /old_locus_tag="HSZ48_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_175268024.1"
                     /translation="MKSVKMLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDIE
                     KIIQRVNENKKKYGFESATEDKQKKESDNINVESKSDTQIVEILKNQIDTLNDQIEKQE
                     SRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEEERQLNYSFDTSVNDR
                     QNVNSQEATFTEESKDINQEQEDEQPTEVQHKDVAEDKKDDSTNEEVLAKDDKPSEEIE
                     DETVDRGEQTPKKGFWSRLFGN"
                     /name="DUF536 domain-containing protein"
     gene            <14073..14147
                     /locus_tag="HSZ48_RS13465"
                     /old_locus_tag="HSZ48_13510"
                     /pseudo=""
     CDS             <14073..14147
                     /locus_tag="HSZ48_RS13465"
                     /old_locus_tag="HSZ48_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /name="IS6 family transposase"
     gene            14529..15251
                     /locus_tag="HSZ48_RS13470"
                     /old_locus_tag="HSZ48_13515"
     CDS             14529..15251
                     /locus_tag="HSZ48_RS13470"
                     /old_locus_tag="HSZ48_13515"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_175268025.1"
                     /translation="MKKILTTSIATIGILTMSIAQQDAQAEEQSSVSTQNTQTSTGIEN
                     GVYYTIDSKGNYHHTLDGNWNQSMFDRKEYKSYELDQNGVTHYYYFEKDDNDYSPSQSN
                     QSIKDKGYQVNNGNEDQTSETNKETIANNNQIDKHDETGSNGHVATSNETNDNQETAQY
                     NHTASKEQSTHNNDATSTKEAKGNAEVSSNWLTKNKKLQEEDQTSETNKETIANNNQID
                     KHDETGSNGHVATGSVAK"
                     /name="hypothetical protein"
     gene            complement(15293..15967)
                     /locus_tag="HSZ48_RS13475"
                     /old_locus_tag="HSZ48_13520"
     CDS             complement(15293..15967)
                     /locus_tag="HSZ48_RS13475"
                     /old_locus_tag="HSZ48_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS257 family transposase"
                     /protein_id="WP_001105987.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYALSYRDISEILRERGVNVHHS
                     TVYRWVQEYAPILYQIWKKKHKKAYYKWRIDETYIKIKGKWSYLYRAIDAEGHTLDIWL
                     RKQRDNHSAYAFIKRLIKQFGKPQKVITDQAPSTKVAMAKVIKAFKLKPDCHCTSKYLN
                     NLIEQDHRHIKVRKTRYQSINTAKNTLKGIECIYALYKKNRRSLQIYGFSPCHEISIML
                     AS"
                     /name="IS6-like element IS257 family transposase"
     gene            16066..17412
                     /locus_tag="HSZ48_RS13480"
                     /old_locus_tag="HSZ48_13525"
     CDS             16066..17412
                     /locus_tag="HSZ48_RS13480"
                     /old_locus_tag="HSZ48_13525"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="WP_175268026.1"
                     /translation="MTKKYDLIVIGTGSAGSITAAKCNKAGWNVAMVDDRPFGGTCALR
                     GCDPKKVLHGAAELIDWNKRMVKNGVPSEVSINWKDLMNFKRTFTDDVPEKKEEALNKQ
                     GIDTYHGNASFVSEDKLEVNKEVLEGSHFLIASGAKPTPLPIKGEEHLTYSDEFLELDE
                     LPQRIVFVGGGYISFEFAHIAARAGSEVHIIHRGQRPLENFDIDLVDILLEKSKEIGIQ
                     VHLQHSVESIEKEQGKFHVYARKKEDITRFEADIVIHGAGRVPALDMNLEKGNIERKKH
                     GVHVNEYLQSVSNPNVYAAGDAAATDGLPLTPVASTDSHVVASNLLKGNSKKIEYPVIP
                     SAVFTVPKMASVGMSEEEAKNSGRNIKVKQKNISDWFTYKRTNEDFAAFKVLIDEDSDQ
                     VVGAHLISNEADELINHFATAIRFGISTKELKQMIFAYPTAASDIAHML"
                     /name="NAD(P)/FAD-dependent oxidoreductase"
     gene            17712..18119
                     /gene="merR"
                     /locus_tag="HSZ48_RS13485"
                     /old_locus_tag="HSZ48_13530"
     CDS             17712..18119
                     /gene="merR"
                     /locus_tag="HSZ48_RS13485"
                     /old_locus_tag="HSZ48_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000525462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hg(II)-responsive transcriptional regulator MerR"
                     /protein_id="WP_002380758.1"
                     /translation="MEMKISELAKACDVNKETVRYYERKGLIAGPPRNESGYRIYSEET
                     ADRVRFIKRMKELDFSLKEIHLLFGVVDQDGERCKDMYAFTVQKTKEIEQKVQDLLRIQ
                     RLLEELKEKCPDEKAIYTCPIIETLMGGPDK"
                     /name="merR"
     gene            18136..18489
                     /locus_tag="HSZ48_RS13490"
                     /old_locus_tag="HSZ48_13535"
     CDS             18136..18489
                     /locus_tag="HSZ48_RS13490"
                     /old_locus_tag="HSZ48_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746200.1"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="WP_000746201.1"
                     /translation="MKKRISFTAIMTVLLIGLTACGAESDTANESKVQDIQGNPVSLPN
                     EKPTLIYFMATWCPSCIYNEEIFKEIHQLNPNDVQLITVSLDPNTDTKESFAKFKQDYG
                     GDWPHVLKIGVEL"
                     /name="TlpA disulfide reductase family protein"
     gene            18486..19166
                     /locus_tag="HSZ48_RS13495"
                     /old_locus_tag="HSZ48_13540"
     CDS             18486..19166
                     /locus_tag="HSZ48_RS13495"
                     /old_locus_tag="HSZ48_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000010351.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="WP_000010351.1"
                     /translation="MSFSFLFILTAGMVAAFNPCGIALLPSYISYLIGGETKDHSFRYA
                     IFKGLGLGGAMTTGFLTIFVLAGLLIGGLGSALTGIFPILSLVMGILIALLGLGMLFGK
                     HLPIKIGSFQVKPGKWSIYFYGIAYAVTSLGCTLPAFMLVVSASLNDNSVTAVIIKFII
                     YSLGMGIVVTAITMVSLISRQLVQKFLHNYMGSIQKIAAVVIFLSGLYMAYYWYFGSGG
                     ICTF"
                     /name="cytochrome c biogenesis protein CcdA"
     gene            19301..19639
                     /locus_tag="HSZ48_RS13500"
                     /old_locus_tag="HSZ48_13545"
     CDS             19301..19639
                     /locus_tag="HSZ48_RS13500"
                     /old_locus_tag="HSZ48_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000968575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000968575.1"
                     /translation="MMDENRSKGNRWGVWAFFGILLVPLLVPLLCCAGPILLVALGSTG
                     IGALFAGATGNWWLTGIFAALAIVMIALILSKLLKNKYNSPEGNGKTKNKTDCCTPPES
                     VDRKHETR"
                     /name="hypothetical protein"
     ARG             19275..19636
                     /name="merT"
                     /gene="merT"
                     /gene_name="mercuric transport protein MerT"
                     /tool="amrfinderplus"
                     /drug_class="MERCURY"
                     /identity="99.17"
                     /coverage="94.53"
ORIGIN
        1 ttcaatcggg atggcgatta cgatcttctg attattggtt ccggcggtgc ggcgttttct
       61 gcagctatca aggccaatga aaacggggcg aaagtggcca tggtagaacg gggaaccgtc
      121 ggggggacct gcgttaacat cggttgtgta ccgtcaaaaa ccatgcttcg tgccggtgaa
      181 ataaacggtc tcgcccaaaa caatccgttt accggacttc aaacgagtac cggtgctgcg
      241 gaccttgccc aattgaccga acaaaaagat ggattagtca gtcaaatgcg taaagaaaaa
      301 tatatagact tgatcgaaga atatggattt gatctcattc gtggcgaggc ctcgtttatc
      361 gacgataaga cgatacaagt gaatggacaa aacatcacgt ctaaaagctt tttaatcgca
      421 acgggggctt ctccggctgt tccggaaatc ccgggaatga atgaggttga ttatttaaca
      481 agtacatccg cactcgaatt aaaagaggtt ccacaacgat tggcagtgat cggttctggc
      541 tatatcgcag cggaattagg tcaaatgttt cacaacctcg gaacagaagt gactctcatg
      601 caaagaagcg agcgtctgtt taaaacctac gatcctgaaa tttccgaagc catcgatgaa
      661 tccttaactg agcaaggact taacctgatc actggggtca cttatcaaaa ggttgagcaa
      721 aacggtaagt cgacaagcat ttatattgaa gtgaacggtc aagaacaagt catcgaagcc
      781 gatcaagtcc tcgtggcaac aggaagaaag ccgaacacag agactttaaa ccttgaatca
      841 gcaggtgtga aaacagggaa aaaaggcgaa gtgctgacca atgaatattt gcaaacgtcg
      901 aataaccgaa tatatgccgc gggcgatgtg accctcggtc cgcaattcgt ttatgttgca
      961 gcttatgaag gcgggattgt ggcaaataat gcgttgggtc tagcgaaacg caaaatcgat
     1021 cttcgctttg ttcccggcgt aaccttcacc aatccatcga tcgccacagt cggcttgact
     1081 gaacaacagg caaaagaaaa aggttacgat gtcaaaacat cggtccttcc gttggacgct
     1141 gtaccgcggg ccttagtcaa tcacgaaaca acaggggtct ataaacttgt agtcaacgcc
     1201 caaacccaga aattgatcgg ggcgcacatt gtgagtgaaa atgctggaga tgtgatttat
     1261 gcggcaacgt tagcggttca atttggattg accattgaag accttacgga tagctttgcg
     1321 ccttatttaa cgatggctga aggattaaag cttgcagcct tgacgtttga taaagacgta
     1381 tcgaaattat cttgttgtgc aggctaatgg ttccttttga ttctaatgct atgttgcagg
     1441 aggcacttaa aacaatgaag tggagaaaat ataaggagga ataagcttat gaaaaatatt
     1501 tcagaattct cagcccaact taatcaaact tttgatcaag gggaagccgt ctctatggag
     1561 tggttattcc gtccgttgct aaaaatgctg gcggagggcg atccagtccc cgttgaggac
     1621 atcgcggcgg agaccgggaa gcccgtcgag gaagttaagc aagtcctaca gactctacct
     1681 agtgtggaac ttgatgagca gggccgtgtc gtcggttatg gcctcacact ggtccctacc
     1741 ccccatcgct tcgaggttga tgggaagcaa ctatatgcat ggtgcgccct tgacacactt
     1801 atgttcccag cactcatcgg ccggacggtc cacatcgctt cgccttgtca cggcaccggt
     1861 aagtccgtac ggttgacggt ggaaccggac cgcgttgtaa gcgtcgagcc ttcaacagcc
     1921 gttgtctcga ttgttacacc agatgaaatg gcctcggttc ggtcggcctt ctgtaacaac
     1981 gttcactttt tcagttcacc gagtgcagcc caagactggc ttaaccaaca ccctgaatcg
     2041 agcgttttgc ccgttgaaga tgcctttgaa ctgggtcgcc atttgggagc gcgttatgag
     2101 gagtcaggac ctactaatgg gtcctgttgt aacatttaat cttaggcaat tacgcctgcg
     2161 tcaatcttgg ccttacgatg atgtcctgta accgaagctc tatctagtga aaccaatttt
     2221 atgggaaacc aaataaataa aaagatcaat tgcttgtgcc tatatcgggg agttaataaa
     2281 gcgagaaagt ggattcttga atataggagt aaataaggtg ccaaaatggc accttattta
     2341 aaatatgtaa cgccaaggtc tgccgtatta tattgcattg gaaaaagaaa accttctgta
     2401 taaggagatg ttatatgata tttggtaaac tgttcggcag tttgggcgta gaaagctttt
     2461 gctccttgaa catcacgttt gccagttaca atgtcattta acaagtttaa agataaaaaa
     2521 ttcatcgctt cctcatgaca ctttgctgat gcctcacctg tagttcgatc taagtagaca
     2581 cttccatcaa aagcggcaat cgcatcatat aagtgtacgg gcactttata atcggttctg
     2641 ttgcaaagta aaaaaatata gctaaccact aatttatcat gtcagtgttc gcttaacttg
     2701 ctagcatgat gctaatttcg tggcatggcg aaaatccgta gatctgaaga gacctgcggt
     2761 tctttttata tagagcgtaa atacattcaa taccttttaa agtattcttt gctgtattga
     2821 tactttgata ccttgtcttt cttactttaa tatgacggtg atcttgctca atgaggttat
     2881 tcagatattt cgatgtacaa tgacagtcag gtttaagttt aaaagcttta attactttag
     2941 ccattgctac cttcgttgaa ggtgcctgat ctgtaattac cttttgaggt ttaccaaatt
     3001 gtttaatgag acgtttgata aacgcatatg ctgaatgatt atctcgttgc ttacgcaacc
     3061 aaatatctaa tgtatgtccc tctgcatcaa tggcacgata taaatagctc cattttcctt
     3121 ttattttgat gtacgtctca tcaatacgcc atttgtaata agctttttta tgctttttct
     3181 tccaaatttg atacaaaatt ggggcatatt cttgaaccca acggtagacc gttgaatgat
     3241 gaacgtttac accacgttcc cttaatattt cagatatatc acgataactc aatgcatatc
     3301 ttagatagta gccaacggct acagtgataa catccttgtt aaattgttta tatctgaaat
     3361 agttcataca gaagactcct ttttgttaaa attatactat aaattcaact ttgcaacaga
     3421 accacttaat gtatatctta aatagtagcc aacggctaca gtaataacat tccttttcgt
     3481 tgaaattata ctataaaatt tactttgcaa cagaacgaag actcacctac aaaatcaatt
     3541 gaacatttaa aaaatatagt tacgataact ataaagatac acattctaaa aaaatataaa
     3601 tgttttaaat acttatatag gtactatatt gtttatcttt acattagttt caagtattgc
     3661 acttgcaact aaatttattg tgtttatcgt gattttcgtt tttctagtag ttaacgctat
     3721 aatgtagtat atgatgacaa ggaagcgttg aatataattt acttttacat atttatataa
     3781 aattacatca tatttcctta ttaaaaatta atattattaa gaaaaaatta taggaaaagc
     3841 ggtagcggat aagctctctc tttttttatg attagaactt gataatagtt tattaacatg
     3901 tgttttaaga tgctgtgaga aaccttaatt tgcattaaaa aagcaattat taagtcaaat
     3961 tattatcatg tttaattcta taaactgttt gtcttgttat attaacttct tttgaaatct
     4021 tagtaattga ttcacctctt tctaacattt ccacaactcg atgataaata atacgctttt
     4081 gaggatcttt cgcattaggt gaatatagta atggccgtcc tttatatacc cctttttctt
     4141 ttgctacttt aatcccttgt gcttgtctac gtttactttc atttcgttct tgttctgaaa
     4201 ccattgctaa tatttgaatg attaaatctt tcataaattt atctaataat ggattaccaa
     4261 tcacttcatt catcataggt aagctagtaa tcatcaattg aacttctttg tcttttaaat
     4321 aattaactgt gttaatcacc tcatcataat tacgacctaa acggtcaata gactctacaa
     4381 caaatcgatc tcccattctc acaaagttaa gtgcttcttg aaatacaggt ctattttcaa
     4441 ctgatttgcc tgattgcttc tctgtaaata ttttctctac gccaaatgtt ttcaaattat
     4501 ctagctgtct ctctagattc tgatctatcg aagatactct cgcataacct ataatcattc
     4561 attttcactc cataattaat atacacccta atcatacgtt taagttacta taaattcaag
     4621 aacttattct gtatgattag ggtgtaccct taatttatat ataaattgtt ttttttaagt
     4681 agctttaagt tttgtagtgt catcccaatt agaaagaatt tctgaaaata taataatact
     4741 aaaaatatca atagataata tagataaaaa gcaattaatc caaatagcaa cgatatttaa
     4801 tataagttgt gataatatta tattgataaa aggaaggtgg atattatgga ggtaaatcta
     4861 caaaaaaaat caatagaatc aattcaacat tcacatttaa taggattatc attagtgaat
     4921 ttcattattt ttgcagttta ctattttata tatgcaggtc caggtataaa agggaatatg
     4981 cctttatata tgaaagtata tttaatagga tcattattat tatggttgct tctttattta
     5041 caagaacgtg agtatatttt taaacattat aatcgaaaaa attataaatt cagagttgta
     5101 atatatatga ccatcaatct actttcagga tacaatattc catttttaat tagttctgct
     5161 tatgcttttt attttgcaga ttctagagat gatactttta catattggtt gttaatttgt
     5221 gggctcataa tagtctctgc tataggtcta ttcttatttt gtctaggtga atttgagatg
     5281 tttggtatcc aaaacggtag ttttattaga atgattggaa ttttaactat tttattatca
     5341 tttggactat tgctgtatgt atcattaatt cttcctgttg attctgaaga aaatagaacg
     5401 atttggatgg gaatgataat gttattttgt agccatttat taattggacg aacatatttt
     5461 tatttaggtc tcttaaatta tgacattaaa gaagatggga tgaaattata atagaattag
     5521 gtatgaatgt aatgccttat ttcgtcaaaa taacatttga aattaataaa gaagaaaact
     5581 ggttaaaagg tttagataca gtaaatgaat tttataattt ataactataa atagctaaca
     5641 ggacctataa tatttcaaag ggagcttatt gggattaatg aatatcagaa tacagtgttt
     5701 atttctttaa atgacaggtt acaacatatc tctgaattaa acatagaata tattgatatg
     5761 ttaaaagttg accctactct atcaaaaaaa tgttttaata gtgaacgata taaaaattga
     5821 tgtgaaacaa aagcaaaggt atatgagaat aattaaggaa gtgtaagtat tggtttattt
     5881 tgtactgatt ggagtattta ttatatttta tttagctcta tttgacagtc ttctgaaatc
     5941 tgaaaatttt tcaattttat ctattttttt agattttgtt attgtaataa tgttgattat
     6001 ttttcatttt attggaaata aacttacagg aaatgattta agtaattata tagcatttac
     6061 tttaattggt tcatttgtgt atatgtattt tgcaatcaag aatttttgga caaaacctaa
     6121 agttttgaac tatctaatta gtaaaaagga aaatacaaat caagatgata tagaatatca
     6181 agagttagat ttacaagtct cgaaaattaa atctgtttac tattttttaa ttgctattgc
     6241 acttttaata cttacgaaag tacatattgt tagtgatatt aaagcagaca gtttgtcaat
     6301 gaaccctgta tttatttttg ttggaatcat tattatagtt atctggctaa taatggactt
     6361 gtatagaaag aaaaaatatg gcattttttt atttaaatca ttaattccta ttgtcgtgac
     6421 gatatggatc ataatgtcat ctgtattatt gtattaacta tagtaacgcc ttattcgcga
     6481 ttggaagtat cacttgcagt gttaagtata aataatattt agaacaattt agacattaag
     6541 tcataattct aatatttata gaaataacga atactttgaa tcattataaa atatttatta
     6601 tatacatttt tgcattccta cgtactatga tgttataatg cacttaacaa ggaatccagc
     6661 atggatacac caatcccctc actaccgcaa atagtgaggg gatttttgta ggtgtggcta
     6721 ttgtcgccta tttgtctttg cgattacgca gccaatgcgt aaataacgca ataatacaac
     6781 cactgatgac tgtggtcgtg atgtgaacaa ggatttccaa catgaataac accccctccc
     6841 tacgtcaaat tgacgcctga gagataggcg acgttactat tataccatct gtatcttttg
     6901 aagcatatct acttccgata agttgaatta tgtcagctca atgataaaat aaatatactt
     6961 gttttagtaa aaataagttt tcacttttcc ccacatacta ctctattatc actccatccc
     7021 ttttcaattc ttttcttttt catagatttt cctaatttaa aatactacaa gtagaaaata
     7081 gaagtgtata ttaaatatct ttaaaattag aaaggatgtt gaaattatat attattaaaa
     7141 aacttgtaaa tatttgaaag gaatggattg aaaatgccga aagaaaaaat aactagagaa
     7201 gaacttggaa agcaaatgat ggaaactact ataataaaat caaatataaa aaaagtaaaa
     7261 aacccttcga atgatcaata tgaaagtctt agatcaagaa catctattaa aatacataga
     7321 gatttcaaac cattatttga tgaattagta gcagaagaac gtgaaaataa aatagatttt
     7381 gtagatgcat tattagtatt ttatgctcag gaaaaatatc cagagatttt aaaagccttt
     7441 aaaaaacaag aattaaaaac tcaaaagaaa taaaaaagga atctataaat acattagtat
     7501 ttattctcac tattcatttg cctattaatg ttcctttata agcaataaat aatccataga
     7561 ggaagataca tttaaatgaa aatgaggcac tacacatcta aaagataaaa cgcttaacag
     7621 aaataattaa tctgttaagc gttttattta ccttaatcaa agaaatcata aattaatgat
     7681 tgctgaagga ggagtctttg atctttcttc aatatatata aattataaca cattttcgta
     7741 tcatcaaata tcaagaataa ttttgaaaaa ggataaatta gaaaatcaaa aatgaataaa
     7801 cttctaattt tttcatacca aaatagctcc ttataaattt aaaaagcgta aaaattaaca
     7861 taaaaatttg cggttttctt tattgctttt taaattttaa gtatcaatta ataacacaaa
     7921 ttcgttttct atacctattt aaatttcgtt tttgatattt cattttttca aaaatggctt
     7981 attctagtag aataaaactt caaaattctt tattctacta gaataaaagg gtctctgaag
     8041 ccctttaaat aggctttatc tataggatta gggttcacat taacaatgcc attcgtaatt
     8101 tatagaataa aaattacgaa tggcattttt gtctaatgta aatgtgctat aaacactagt
     8161 ataacagcat ttatagcgag agcaaatgac gaaggaatgg taagaaaaat acatcagcta
     8221 tgctgattga cacgaaagtg gcattgtatt tccttatgct cttatttttt attctgcttt
     8281 cagcataaaa aataaatgat ttgtacaaaa gttaaatgaa taaaaatgat tttttcgcta
     8341 tcataatgac tcattctttt cgtaccaaat cactcaagtt tatattttta attaaaagat
     8401 gttacgatac gtgatcaaaa gaacgtaaga gtaactttat ttagctgttt acagcaaata
     8461 aacgtatatc taatgtactg ctaagaagaa gattagatat accttactac ctttagaaaa
     8521 ttcaattttt aactttaagt tagtaaatta ttattcatta atttaaaatt tgtctaaaat
     8581 catcaacagt atatataaag tatattttcc cttcttggtc ataccattat ttaaacgtag
     8641 ggttaaatgc aaaccagaag tctgacgata acttcaagag tttctaggaa gttttttgga
     8701 atattagtaa ttaaggcctc tcttttttag ataaatgtca gatttatttt tgtatttaat
     8761 gggagtgctt cacccctaat gtaaacattt ttattatttt agtttacatt tagaacgctt
     8821 agttttatta tcagtactaa tacagatata ggaaagagtg gtgaaatgtt tgcttgtaat
     8881 catgaaaagg tgcgctagat atcatgtgtc taggtaagac gattacgggg gggatttacc
     8941 tttagctgct actttaaatt ctcaaaagat ttataaaaca ttttcaagcg ataatcatgg
     9001 tgttaatgca tttttacata gctatactta caccggtaac caaatagtat gttccattgc
     9061 tttagaaaat attgttactt ttgttccaat tttaagcata ttaaataaag aaatttaaaa
     9121 aatggttaat atttttgaaa aagcactaaa caatatatta gatgaggatg attaatatga
     9181 atttagctga aaagatttta aaaggagaaa ccttaactaa agagaggtta ttgaaaatat
     9241 atgaaaatcc taatatagat acattagatt tattgaacga agcctatata ttaagaaaac
     9301 attattatgg aaaaaaagtg aaattaaata tgatcttaaa tgctaagagt ggtatatgtc
     9361 cggagaactg tggttattgc ggtcaatcta gagatatgaa acaaaagcaa agatacgctc
     9421 tagtgccaga agaacaaatt gttagcggag ccaaagtagc tcacgagaat aacataggta
     9481 catattgcat tgtcatgagc ggccgaggac ctagtaacaa ggaaattgat catataagta
     9541 gaacagtaga agaaattaaa tcaaaccatc ctcaactgaa aatatgtgcg tgccttggtt
     9601 taactaatga tgaacaagct aagaagctca aatcatctgg cgttgataga tataaccata
     9661 atataaatac gagtgaaaac tatcatgaag aagttgtaac tacccatact tataaggaca
     9721 gaacaaacac tttagaaatt atgaaagaaa acaatatttc accatgttct ggtgtgattt
     9781 gtgggatggg agaatcaaat caagatatca tagatatggc ttttgcctta aaagaaatag
     9841 atgcagatag tattcctatt aattttttac atcctattaa aggtacaaag tttggagaaa
     9901 tggatgaatt gacacccatg aagtgcttgc gacttatatc tctatttagg attgtcaatc
     9961 catctaaaga aattagaatt gctggcggta gagaagttaa tttgcgttca ctacaacctt
    10021 tggccttaaa ggcagcaaat tcaatatttg taggagacta cttaattact ggaggacagc
    10081 ctaataaatt agattataag atgatagaag atttgggatt tgaaatcgac tctggatata
    10141 atgagctgaa agaaaaagta gaatgacctt acaaagtgat tttaaaaatt acaagaagaa
    10201 gatttatata gaaagattaa atctattgaa tccattcatg gtaaagatat acctgtagat
    10261 gggaaaatga tattaatttc gcctccaatg attatcgata caagaagaaa ctcgtttcac
    10321 tcgttttaaa aaccacaaat tttgacacta acgtaacgta attagttaca ataaaattaa
    10381 aaaaggtgga gaaaaacatg gatttttatc aaatgccaat gttcaacaaa atattagtac
    10441 aagacattga aaaagcagaa aaatggtacg agaaaacact tggctttaaa agtgttttca
    10501 aattcagaaa cgaaaaaaac gaagtactaa tgaatcactt aagattagaa aaatatcaag
    10561 atttaatgtt gatttctagt aatgatttta aagttgggaa ttcaatatat ctaaacttat
    10621 tagttaaaga tatagaatct aaatcacaaa caatttcacc aaaatttgtt atttccgaac
    10681 tcgaggaaca accttggaac gcaaaagaaa tgactataaa agacccagaa ggtcatttga
    10741 tcacacttac tcaatcaaat atcagcgaca ctgattttga aaaattaatg caacaaacat
    10801 caaaaaacta ttaacaacaa aattccaatt gctttattga cgttgagcct cggaaccctt
    10861 aacaatccca aaacttgtcg aatggtcggc ttaatagctc acgctatgcc gacattcgtc
    10921 tgcaagttta gtcatggatt ggctttttat ttttatatag cctcatttag attctaaagg
    10981 ggcatttcaa aggatttaga gttacttata tagatttata cctaaagctt atatacgttc
    11041 ttaaaatgca aatatgagcc aaataaacac atcttaattt cacaaaccaa tttttgatac
    11101 aaacgcgttt tgatttttta atgcgtttaa ctttaacaaa ggattgagta tataaataat
    11161 ctctcttagt attcacttct caaaaaacca tagattaatg tatctctgta tgtatcttgc
    11221 actaatttat cttgtctcaa aatgccctct cgtttcatac ctaacttttc caacacaatt
    11281 cccgcagcta tattgcttga atcaatggag gcccatattc tattgagttt cagtacttta
    11341 aaggcatatt tgataattgt ttttgcgata tccgtcgcaa tgccgttgct ccaatgattt
    11401 gggtggacaa taaagttgat ttcagcactt tggttcactt catctatggc taattgtatc
    11461 gtccctatga ctttatctgt atcaggatca acgaggacat tgtaaatcca attcgaatct
    11521 ttatctaaaa ccatattaat gaattgctga gtctcttcat aactttgagg accccaagtt
    11581 tgatactgtg tcacttctgg taaagcatat aattcattta aacgatctat atcagtattc
    11641 tttaagtctc tagaatagaa ctccatacgc gccctcctaa aaatagttta atcctatgta
    11701 accctttatt tactagctac ctatgatata aagggcgttt ctcttaattt aaatgtgaac
    11761 gcacgccaaa tttattgatt aaagatatta tgttgtttca tgttttgacg acgcatatta
    11821 agtgcgtgta tttcttcaag ttcagcttta atcgattgca ttaaatatcc ttgcatagct
    11881 tcaactgttt cattcttttg tagcagcatc gctttaaaac gtttcaacac acttacaatt
    11941 tcaaactcaa catcttctaa acggtaataa gtatcatgtg cattattaaa tgatttcttc
    12001 cctttgagta aaacactttt aataattcga atttctctaa tttcaaattt acttaaatag
    12061 tcttgaagtt gttgcggcaa ttcttcgagt acttggaact taagcgcatc attattaaat
    12121 tcggtttgtt gatgatttgt atgatccgaa tgagtgtttt ttattggatt gttgtatgta
    12181 tcattcatat catgcgtatc attattctca gtctctatat aatcagtatt acttaagtca
    12241 gtatcattag ggtctaagtt ttgggattct gggctcccat tatttgggat tctggactcc
    12301 cattgtttgg gattctggac tcccaaacaa tgggagtcct tgctatcact tgattcgtca
    12361 ttgtttgaag tgctgttttg tatttctttt tcaatattta tatttgtttc aatttcatga
    12421 aggtatagtt tgtttggtcg attaaatcca gttttaactt gttctaataa acccacttct
    12481 tgtaattctt tttttatttt agttattgta tttttacttt tatctaatac ttcttgaaga
    12541 ttttcatttg taaaaataaa atatatatct ccattttgat ctatccaatt atttttaata
    12601 gataagctta ctctatcatt aagaattgca taggtaactt ttgcactatc acttaacttt
    12661 ttatattttt cattgataaa taatacttta tgtagcatat agaaattttg actggcctga
    12721 ttttttatgt tttttctaga cataataaaa acccctttcc tattcaaatt ttggatagag
    12781 gtaataaaac attacaagaa cacataaatt ttttgcttgc actttgatga tatagttgta
    12841 tataatacag atatcaaaat atttataaac gtgctgcaac gttttattac caaaccccga
    12901 ctattaccag tagttggggt ttttgttgta ttaaggttat tatagcactt tgaataagct
    12961 tataaaaggg aaaatgtagg attatcaagg gttgataaaa ggtttataaa cccttgataa
    13021 ctatcatcaa ccttttatca agtatttgta aaaggttgat gatagtttta tatttttgat
    13081 ttattaatcg ctttattatt aagcgtgata agcttataat attttataaa gggttgataa
    13141 acattttata aagggttgat gatatgaaaa gtgtgaaaat gttgagtgaa gaattagggg
    13201 ttagtaagca aacgattttt aataacatta agcgattgaa tatagagaca ataaaacagg
    13261 aaaacacttc gtttattaaa gaagataccg atatagaaaa aatcattcaa agagtaaatg
    13321 aaaataaaaa gaaatatggt tttgaaagtg ctactgaaga caaacaaaaa aaagagtcgg
    13381 ataatattaa tgttgaatcc aaaagtgata ctcaaatagt agaaatcctt aaaaatcaga
    13441 ttgatacttt gaatgatcaa attgaaaaac aagaaagtcg tcatgaaaca acaattgaat
    13501 tctacagaaa agagttgcag gaaagatcaa aattgcttga gaatcagcaa gtattggcat
    13561 tagagagtaa taagaagatt caaaaattag agaatcaatt agaggaagaa agacagctta
    13621 attattcctt tgatacatct gttaatgata gacaaaacgt taattcgcaa gaagcaacat
    13681 ttaccgaaga atctaaagat attaatcaag aacaagaaga tgaacagcca acagaagtac
    13741 aacataaaga tgtagctgaa gataaaaagg atgacagtac aaacgaagaa gtattagcaa
    13801 aagatgataa accatctgaa gaaatagagg atgagactgt tgatagggga gaacagaccc
    13861 ctaaaaaagg gttttggagt cgtttatttg gtaactaatt gtaattaaaa agtcacgaca
    13921 agttcataaa cttgtcgtga cttttttatg tgaatcgaac ttctgataat catctattat
    13981 gttaattact gtcagatagt agttgaataa acttcaggag atatacataa ctaggttctg
    14041 ttgcaaagtt gaatttataa tataatttta ataaaaagaa ccgcaggtct cttcagatct
    14101 acggattttc gccatgccac gaaattaggc atatgttagc tagttaaggg aacaaagaca
    14161 tgataaatta gtggttagct atattttttt actttgcaac agaacccttg cctctatatt
    14221 tattatttaa atgctagtta attcaacatc taattttatg agtaataaga atgtcccttt
    14281 aggtatcttt tgaaagttat tagattctta acttgtaatg aatttttttg aaaacagccc
    14341 attaaatgta tacaccctat gtgtaataag gaatgacact aatatcaaaa attcataaat
    14401 cagtaatatt attttaaaaa tattacaact ttttatttag tatatacaaa tcgattacaa
    14461 gtatttattt tcatttaagc acgtgataga ttgagttcta tagtttaaga aaaggaaggt
    14521 ttattaaaat gaaaaaaata ctaacaactt caatagcgac tattggaatt ctaactatga
    14581 gtattgctca acaggatgca caagctgaag aacaaagtag tgtatctacg caaaatactc
    14641 aaacctctac gggaattgaa aatggtgttt attatacaat agatagtaaa gggaactatc
    14701 accacacatt agacggtaat tggaatcaat ctatgtttga tcgtaaagag tataaatcgt
    14761 atgaattaga tcaaaatggt gttacgcatt attactattt tgaaaaagat gataatgact
    14821 attcaccatc tcaatcaaat caatcaatta aggataaagg ttatcaagta aataatggaa
    14881 atgaagatca aacatctgaa acaaataaag aaacgatagc aaataataat caaatagata
    14941 agcatgatga aacaggaagt aatggacatg tagcgacttc taatgaaaca aatgataatc
    15001 aagaaacagc tcaatataac catactgctt ctaaagaaca atctactcat aacaacgatg
    15061 caacaagcac taaggaagcc aaaggtaatg cagaagtttc atcaaattgg cttactaaaa
    15121 ataaaaagtt acaagaagaa gatcaaacat ctgaaacaaa taaagaaacg atagcaaata
    15181 ataatcaaat agataagcat gatgaaacag gaagtaatgg acatgtagcg actggttctg
    15241 ttgcaaagta aaaaaatata gctaaccact aatttatcat gtcagtgttc gcttaacttg
    15301 ctagcatgat gctaatttcg tggcatggcg aaaatccgta gatctgaaga gacctgcggt
    15361 tctttttata tagagcgtaa atacattcaa taccttttaa agtattcttt gctgtattga
    15421 tactttgata ccttgtcttt cttactttaa tatgacggtg atcttgctca atgaggttat
    15481 tcagatattt cgatgtacaa tgacagtcag gtttaagttt aaaagcttta attactttag
    15541 ccattgctac cttcgttgaa ggtgcctgat ctgtaattac cttttgaggt ttaccaaatt
    15601 gtttaatgag acgtttgata aacgcatatg ctgaatgatt atctcgttgc ttacgcaacc
    15661 aaatatctaa tgtatgtccc tctgcatcaa tggcacgata taaatagctc cattttcctt
    15721 ttattttgat gtacgtctca tcaatacgcc atttgtaata agctttttta tgctttttct
    15781 tccaaatttg atacaaaatt ggggcatatt cttgaaccca acggtagacc gttgaatgat
    15841 gaacgtttac accacgttcc cttaatattt cagatatatc acgataactc aatgcatatc
    15901 ttagatagta gccaacggct acagtgataa catccttgtt aaattgttta tatctgaaat
    15961 agttcataca gaagactcct ttttgttaaa attatactat aaattcaact ttgcaacaga
    16021 accaggaaaa cttattaaag agtgatttct gtgaggagga taatgatgac aaagaaatac
    16081 gatcttattg tgattggtac aggttctgcg gggtcaatca cagcagcaaa atgtaataaa
    16141 gccggctgga atgtagctat ggtagatgac aggccgtttg gaggcacgtg tgcactacgt
    16201 ggatgtgacc caaaaaaggt tctacatggt gctgccgagc ttattgattg gaacaaacgg
    16261 atggttaaga atggagtacc gtctgaagtc tccatcaact ggaaagatct tatgaatttt
    16321 aaaagaacct ttacagatga tgtcccggaa aaaaaagagg aagcattgaa taaacaggga
    16381 atcgatacgt atcatggcaa tgcatctttc gtgagtgagg acaaacttga agtgaataaa
    16441 gaagtgctcg aggggagtca tttcttgatc gctagtggtg cgaaacctac ccctttaccc
    16501 ataaaaggag aagaacatct tacttatagt gatgagtttt tggagttgga tgaattacca
    16561 caaagaatcg tctttgttgg tggtgggtat atttcgtttg aatttgctca tatcgcggca
    16621 cgtgctggat cagaagttca tattatccat cgaggtcaaa gacctttgga gaattttgat
    16681 atagatcttg tagatatcct tttagaaaag tcgaaagaga ttggcataca ggtacatctt
    16741 caacattccg ttgaatccat tgaaaaagaa caaggaaagt ttcatgtgta cgctcgaaaa
    16801 aaagaggata taacgcggtt tgaagcagac attgttattc atggtgctgg acgggtccct
    16861 gccttagata tgaatcttga aaaagggaat atagaaagga aaaaacatgg tgtccatgtt
    16921 aatgagtatt tgcaaagtgt aagtaacccg aatgtctatg cagctggaga tgctgcagca
    16981 acggatggct tgcccctcac acctgtagcc agtacagatt ctcatgtcgt agcatctaat
    17041 ttattgaaag ggaacagcaa aaaaattgaa tatcccgtga ttccatctgc tgtatttacc
    17101 gtacctaaaa tggcatcggt aggtatgagc gaggaggaag ccaaaaactc tggccggaat
    17161 attaaagtaa agcagaaaaa catctccgac tggtttacgt ataaacggac aaatgaggac
    17221 tttgctgcgt ttaaagtgct aattgacgag gatagtgatc aagttgtcgg tgctcatttg
    17281 attagtaatg aagccgatga actaattaac cactttgcaa cagccattcg ctttggaatt
    17341 tcaaccaaag aattgaaaca aatgatattt gcctatccaa cggcagcttc ggacattgca
    17401 cacatgttgt aagtttgcgt tttgtgagat gtttataaac cactttatcg tctaatcttg
    17461 ttatatgatc ttagaatcaa aaagaaaata acttttttga cagccacatt tttcaacgga
    17521 agaatgtggc ttcttggcgt gaagagcaga tgtctttatt cttaaacttc taaaaaagct
    17581 tatgtgaaca cttgataaat aaggtttttt atctgaccta ttttataaga ttattctata
    17641 aaagaaaaaa taatgtatga cttgaccgtg tactatggta cagggtttat actttttatt
    17701 gaggtgacaa tatggagatg aaaatcagtg aattggctaa agcgtgtgat gtgaataaag
    17761 aaaccgttcg gtattacgag cggaaaggat tgatagccgg gcctcccaga aacgaatcag
    17821 ggtatcgaat atattcagag gaaacagcag atcgggtacg gtttattaaa cgaatgaagg
    17881 aattggattt ctcgctaaag gaaatccacc tgttgtttgg tgtggttgat caagatgggg
    17941 agagatgtaa agatatgtac gcctttaccg ttcaaaaaac caaagaaatc gagcagaaag
    18001 tgcaggattt gttacgaatc caacggttat tagaggaatt aaaagaaaag tgtccagatg
    18061 aaaaggcgat atatacctgt cctattattg aaacgttaat gggagggcct gataaataaa
    18121 tgggggaaga tttgaatgaa aaaacgcatt tccttcaccg ctataatgac ggtgttgtta
    18181 atcggtttaa cagcttgtgg agcagaatct gataccgcga atgaatcaaa ggttcaagac
    18241 attcaaggaa atccagtcag cctacctaat gagaaaccca cactcattta ttttatggca
    18301 acctggtgtc catcttgtat atacaatgag gaaatcttta aggaaattca tcaactaaac
    18361 ccgaacgatg ttcaattgat cacagttagt ttagacccta acacagatac aaaagaaagc
    18421 tttgcgaaat ttaaacagga ttatggcggg gattggcccc atgttttaaa gataggagtg
    18481 gaattatgag tttttcgttt ctcttcattc tgaccgctgg aatggttgcg gcctttaatc
    18541 cttgtggtat cgccttgctt ccttcttata tctcctattt aattggaggc gaaacaaagg
    18601 atcattcgtt ccgctatgca atttttaaag gattagggct tggtggagcg atgaccacgg
    18661 ggtttttaac gatttttgta ttggctggtt tgttgatagg aggattggga agcgcactaa
    18721 cagggatttt tccgattctt tcattggtta tgggtatact cattgcttta ttggggttgg
    18781 gcatgctatt tgggaagcat ttgccgatta aaataggatc ttttcaagtc aagccaggga
    18841 aatggtctat ctatttttac ggaatagcct atgccgtgac atcacttggt tgtaccttgc
    18901 cagcctttat gttggtggtc tctgcatcgc tgaatgacaa tagcgtaacg gccgtgatca
    18961 tcaagttcat catctactcc cttggtatgg gaattgtggt gacagcgatc acgatggtct
    19021 cattgatttc acgacaattg gtacagaagt ttctgcacaa ctatatgggt tctatccaaa
    19081 aaatagcagc tgttgtgatt ttcctctccg gtttgtacat ggcttattac tggtatttcg
    19141 gttctggtgg catttgtaca ttttaaaatc caaccgatca ggtgtaacaa aaaatagatg
    19201 aaagatgtaa aaaagctaaa cgaacaaatt tcaagttagg ctataggcca tgtatgtacc
    19261 ttaaccaaag aataaaattt ttttagaaag agtggtgaga atgatggatg agaatcgctc
    19321 gaaaggtaac cgctggggtg tttgggcctt tttcggaata ttattggttc ctttattggt
    19381 tcctttactt tgttgcgcag gaccgattct ccttgttgca ttagggagca caggtattgg
    19441 tgccctcttt gctggtgcga caggaaattg gtggttgacc ggcatttttg cagcattggc
    19501 cattgttatg attgctctga tcctcagcaa gttgttgaag aacaaataca attctcctga
    19561 aggcaatgga aaaacaaaaa ataaaacgga ttgctgtacg cctccagaga gcgtggatcg
    19621 gaaacatgag acaagataaa tatgagataa atgtacagaa ctgtctatca aactttaaat
    19681 agaaagaggg attcaaatga ctcaaaattc atataaaata cccattcaag gcatgacatg
    19741 cacaggctgt gaagaacatg taaccgaagc attggaacaa gccggagcta aagatgtttc
    19801 ggccgatttc cgacgcggtg aggccatttt tgaactcagc gatgatcaga tcgaaaaggc
    19861 taagcaaaat atttcggcag ccggctatca acccggagag gaagaaagcc agccctctga
    19921 aaacagtgta gat
//
