LOCUS       NZ_CP054785             5596 bp    DNA     circular CON 15-MAY-2024
DEFINITION  Klebsiella pneumoniae strain KP20194a plasmid pKP20194a-p5, complete
            sequence.
ACCESSION   NZ_CP054785
VERSION     NZ_CP054785.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15186437
            Assembly: GCF_013349085.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Hu,L.M., Zhang,X.T., Zeng,X., Chen,Y., Liu,L. and Li,G.Q.
  TITLE     Genomic Characterization of a Carbapenemase-Producing, Extensively
            Drug-Resistant Klebsiella michiganensis Strain from a Renal Abscess
            Patient
  JOURNAL   Microbiol Resour Announc 11 (12), e0082522 (2022)
   PUBMED   36321904
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Zhang,X., Ouyang,J., He,W., Zeng,T., Liu,B., Jiang,H., Zhang,Y.,
            Zhou,L., Zhou,H., Liu,Z. and Liu,L.
  TITLE     Co-occurrence of Rapid Gene Gain and Loss in an Interhospital
            Outbreak of Carbapenem-Resistant Hypervirulent ST11-K64 Klebsiella
            pneumoniae
  JOURNAL   Front Microbiol 11, 579618 (2020)
   PUBMED   33281772
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5596)
  AUTHORS   Zhang,X.T. and Liu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2020) Clinical Research institute, The Second
            Affiliated Hospital, University of South China, 35 Libration Ave.,
            Hengyang 421001, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 10-APR-2020
            Assembly Method                   :: SPAdes v. 3.14
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013349085.1-RS_2024_05_15
            Annotation Date                   :: 05/15/2024 02:38:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,916
            CDSs (total)                      :: 5,795
            Genes (coding)                    :: 5,617
            CDSs (with protein)               :: 5,617
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 67 of 178
            Pseudo Genes (incomplete)         :: 119 of 178
            Pseudo Genes (internal stop)      :: 24 of 178
            Pseudo Genes (multiple problems)  :: 32 of 178
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP054785.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP20194a"
                     /serotype="ST11-K64"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP20194a-p5"
                     /geo_loc_name="China: Hunan"
                     /lat_lon="27.69 N 111.66 E"
                     /collection_date="2019-04-03"
                     /collected_by="Xiaotuan Zhang"
     gene            complement(join(5395..5596,1..125))
                     /locus_tag="HUR98_RS29330"
                     /old_locus_tag="HUR98_29335"
     CDS             complement(join(5395..5596,1..125))
                     /locus_tag="HUR98_RS29330"
                     /old_locus_tag="HUR98_29335"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            complement(483..641)
                     /locus_tag="HUR98_RS29910"
     CDS             complement(483..641)
                     /locus_tag="HUR98_RS29910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            1281..1457
                     /locus_tag="HUR98_RS29335"
                     /old_locus_tag="HUR98_29340"
     CDS             1281..1457
                     /locus_tag="HUR98_RS29335"
                     /old_locus_tag="HUR98_29340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            1447..1791
                     /locus_tag="HUR98_RS29340"
                     /old_locus_tag="HUR98_29345"
     CDS             1447..1791
                     /locus_tag="HUR98_RS29340"
                     /old_locus_tag="HUR98_29345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2000..>2434
                     /locus_tag="HUR98_RS29775"
                     /pseudo=""
     CDS             2000..>2434
                     /locus_tag="HUR98_RS29775"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2469..2978
                     /locus_tag="HUR98_RS29350"
                     /old_locus_tag="HUR98_29355"
     CDS             2469..2978
                     /locus_tag="HUR98_RS29350"
                     /old_locus_tag="HUR98_29355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            2985..3197
                     /locus_tag="HUR98_RS29355"
                     /old_locus_tag="HUR98_29360"
     CDS             2985..3197
                     /locus_tag="HUR98_RS29355"
                     /old_locus_tag="HUR98_29360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            3864..4088
                     /locus_tag="HUR98_RS29360"
                     /old_locus_tag="HUR98_29365"
     CDS             3864..4088
                     /locus_tag="HUR98_RS29360"
                     /old_locus_tag="HUR98_29365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            4072..4338
                     /locus_tag="HUR98_RS29365"
                     /old_locus_tag="HUR98_29370"
     CDS             4072..4338
                     /locus_tag="HUR98_RS29365"
                     /old_locus_tag="HUR98_29370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(4408..4758)
                     /locus_tag="HUR98_RS29370"
                     /old_locus_tag="HUR98_29375"
     CDS             complement(4408..4758)
                     /locus_tag="HUR98_RS29370"
                     /old_locus_tag="HUR98_29375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(4824..5369)
                     /locus_tag="HUR98_RS29375"
                     /old_locus_tag="HUR98_29380"
     CDS             complement(4824..5369)
                     /locus_tag="HUR98_RS29375"
                     /old_locus_tag="HUR98_29380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     Typing          1116..1195
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(533..930)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          1782..3313
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 actgaaagtg aacctgataa tctctggtta ataagatcac ttagactcat tttggtttta
       61 ttcgaaagcc gttgtaagga ttgctttaat cctatatcta gcatgatatt tgttctttct
      121 ctcattaccc ccccttgcta ttgcgttttt tgttgcgcac aaagctacca ttgcgcatta
      181 agaaacgcaa taaaaaagga gggagggagt ggtttgtaca tgatgtgcag tgcgctgagg
      241 gcatcagtgg cgggtgtttt tccgtgggat ggatggagct ccggggataa cagaactgac
      301 gatcatttaa aggatcttct tgagatcctt tttttctgcg cgtaatctct tgcactgtaa
      361 acgaaaaaac cgcctaggga ggcggtttga tcgaaggtta agtcagttgg ggaactgctt
      421 aaccgggtaa ctgggtatac gagaccgcag ataccaaaac tgttctttca gtgtagccgc
      481 agttaggcac ccacttcaag acctctcaat atctctctcg tatatatcca gttaccaatg
      541 gctgctgcca gtggcgtttt tgcgtgtccg tccgggttgg actcaagatg atagttaccg
      601 gaaagggcgc agcagtcggg ctgaacgggg ggttcgtgca tacagcccag cttggagcga
      661 acgacctaaa ccgagccgag ataccagcag cgtgagctat gagaaagcgc cacgcttccc
      721 gaaagggaaa aaggcggaac aggtatccgg taaacggcag ggtcggaaca ggagagcgca
      781 cgagggagcc gccggagaga aatcaccggc atctttaagt cctgtcgggt ttcgtcctcc
      841 tctgacttga gcgtccattt ctgtgatgct cgtcagggga ggcggagcct atggaaaaac
      901 ggcaacgccg catccttttc tccggccctt tctgatttga gcgcctttgc tttatcccgt
      961 tatccgtgag tcggtcacgc ccgccgcagc cgaacggcag gagcgcagct accgagtgag
     1021 cgaggaggcg tcatttcatc tgtccgtgca tggtattgcg gacgctcctg agccacatat
     1081 cacacctgcc acatgaggca ctttcatgaa tcgtgctctc atgccaacat agtaagccag
     1141 tatacacagc gtaaacgctg tgactggttc agggctgcgc cccgaaaccc gctaacacca
     1201 ctgacgcgct ggcgcttgtc caacgccgga gcgacccgga aaacttttcc gtgccgtccg
     1261 gcgtttgcga aggagggata atgcagaagc cgaagaagaa gccgctcgtc gtaaaaaaaa
     1321 gatcctggca tccggcagtg atggcaaatc tgatgcgtca ggcacaggag gaagattacc
     1381 gccgggagca gatggcagag cttgagcgtt cagcccggtc acattttgcg gatatcggag
     1441 gagaggatgg cggttaagcg tacccgtttt ttaggtatcc gggtgaccga tggggagtac
     1501 cagcagctgc tggagcgctg caatggaagg caactggcgg tgtggatgcg ggaaacctgt
     1561 ctcgatacgc gcccggcgcg gtcgttgcgg ctgccgtcca tcgatccggt tttgctgcgc
     1621 cagcttgccg ggatggggaa caacctcaac cagatcgccc ggaaaattaa cggcggccag
     1681 tggtcaggcg ctgaccgggt tcaggtggtg gccgcgctga tggccattga tgccgaactc
     1741 gagcggctgc ggcatgccgt gctggaaaag ggggcagacg atgatcgtta agtttcatcc
     1801 gcgagggcga ggcggcggtg ccgggccggt ggattatctg ctggggaaag accgccagcg
     1861 cgacggggcc atcgtcctgc agggaaagcc ggaggaggtc cgggagctta tcgacgcctc
     1921 gccctacgcc aaaaagtaca cctccggcgt cctgtctttt gctgaacagg atttaccgcc
     1981 cggccagcgt gaaaagctga tggcgagctt cgagcgggtt ctgatgcccg gactcgataa
     2041 agaccagtac agcgtgctgt gggttgaaca ccgggacaag gggcggctgg aactgaattt
     2101 tctggtaccg aacacggaac tgctgaccgg caggcgtctc cagccgtact acgaccgggc
     2161 agaccgtccg cgcatcgatg cgtggcagac cgtggtgaac gggcgactgg ggctgcacga
     2221 cccgaacgca ccggagaacc ggcgcgcgct ggtgacgcca tcttcgttac ccaaaacgaa
     2281 gcaggaagcc gcagaggcta ttacgcgggg cttactggcc ctcgcctcgt caggggagct
     2341 taaaacgcgt caggacgtca ctgaggcgct ggaaagcgca ggttttgagg tcgtgcgcac
     2401 cacaaagagc agcatcagca ttgccgaccc ggacggaggg cgaaacatcc gacttaaggg
     2461 agccatctat gaacagtctt ttaacgctgg cgaaggactt agagcagaaa tcgaaagcgc
     2521 agcagcagag taccggcgaa atgctgaaag ccgcattcag cgagcacgag aagtctgtca
     2581 gagcggaact gagcgaaagc gggaagagaa tcagcgccgc catccgcgcc cacgagcagg
     2641 gtatgagcgc ggccatgcag tcgaaccgcc tgagcgtgat gcgtatggtc gggcggacat
     2701 ggctgaccat cacgatggtg tcaggactgc tgttcgccag cctgagcggg gtgttgtggt
     2761 atcaggggag cttgatagcg tcaaatctgg ccgaaatcga ccggcagaac gcagcgctgt
     2821 cgaagctgaa cgcgaagacc tggggcgtga cgtacctgga agacagcaac ggacgttttc
     2881 tggtgctgcc gaaggggacg gccgtagaca ggacgcagag ctggacggtc gggaacgggc
     2941 ggtcgaagca gaacgcgctg agactggtga aggagtaagt gccgatgacc gagctggaaa
     3001 aacagttgct gagcgcatta gagcagctac agcaggacta ctcgaaaagg ctggacgagt
     3061 gggagagcgc cttcggggaa tggcggaaaa tgtcagggct tatgcaacgg gagaacgcgg
     3121 cgctgagcga gcgcgtgacg cgcttgagtc agcaggtgga acgcttgagc gggcaactgc
     3181 agcgtttgag ccggtagtgc agcgtcatga gatggccgtg gccgcagaac gggcgcacga
     3241 gcaacggcag cacgaaaagg aactgacgga agcgcgttcc cggcagcgaa gctatgacgg
     3301 tccgtcgctg ggctgaaatg caggaaaaca gggttctcat cgggcattta cgctgaaacc
     3361 ggatgtcatt cacctggctg catggctatg cagccaggta aaatttatct gtcgcgaacg
     3421 gggcggttta tcgggtggtt tgttgccggt tttacctgac tgccaccccg ttcgcggcga
     3481 acccgtccgg ggcggtgcgg gcaacgggtg ttatgtttaa tcagcgtgaa cggcagatac
     3541 aaaaaagccc cggagggctt ttaactactg acttattttt ttgaagattt gctgggtttc
     3601 aatttgtcag tatttcccgg cgttttttta ttgtcacgct ggcgtttatc aggttcccct
     3661 gttgaatcgg caattcgttt cggacccggc ttaagaccca taatcgttat cctttcaata
     3721 gttaagagga aagccctcaa gctagatata ttgaccgcgt tacggattat caagaggggc
     3781 taacaagatt attttttgtt cttttaggtt gattgtggta ctgttgatat acatgtatag
     3841 caaatggtgt tcgggaggtt tatatgcttg ctatccggtt atctgatgag attgagtccc
     3901 gtctggactc gctggcgaag caaaccggca gaacaaagac gttttatgcg cgggaagcaa
     3961 tactggcgca tctggaagac ctggaggatt attatctttc agcagaaact gctgcacgcg
     4021 ttcgccgtgg tgatgaagca gtgcattcgt ctgaagacgt gaggaagtca cttggtctgg
     4081 acgattaact attccgatcg ggcgctcaaa tcgttacgca agatggacaa acagaacgca
     4141 cgacggattg tggattttat gagtttacgc attgcagttg ctgccgatcc tcgccagtca
     4201 gggaagccgc tcaaaggtga gctgggcgag ttctggcgct atcgggtggg agattatcgc
     4261 gttctgtgtg agatccgaga tgacgagctt gttatccttg ccgccacgat tggacatcgc
     4321 cgcgaagttt acgactgaag cccgctatgc gggctttttt gatgccgaca acaggtatta
     4381 tggtaaatac tcgactaact acgtgttcta ctgtttgtcg gttttagtta accagcaggt
     4441 tgtcttaacg ataatgtagc cgttattttt aactaataag gtcatattgt taacgtcagg
     4501 cataataaaa gacattatgg cctcagagtt atcatcagaa gcagctctct gcattactac
     4561 ttcgattccc ttttctttga atctataagt atccagttgc ctattaaact tagaaagacc
     4621 tacaaccata cttagtttgt tatctttaac agtaagagat gcttcctgag taccattaca
     4681 tcggtagtaa gcggtgtaac cgtcagcaaa agatcgaccg gggaatataa atgctaggaa
     4741 cgcaattaac caatacatag ttgagaatct tggtattaca catttcacta tcctctcttt
     4801 catttttcgt tcaccatact ttgttatctg atttttaatt tttctatttt ataaaccaag
     4861 tctctaccaa aaaggcgaaa attatcctca aataataata attgtttaag aacatccatg
     4921 aaagcagctg cttcaccctc agttatggga gctactctat ataacatata acctgagtta
     4981 agggtttctt ttatactttc ttcaatgctc attataaata ttagtggatt atcatttttc
     5041 ttcccccatg ggtttctaat taatccttcg atgtactcag gtcctttgcg tcctattgcc
     5101 tgcgcaatat tttttatttc atctgcgtga gggctttgtt gtacagcttt gtttatgcct
     5161 gcttgagtga ttttcagaga ctctacaccg tgtatgttgt atgagataaa atgtaataaa
     5221 ttaagatgca atgcaattaa tccatttttg tttacatctg agtatttgga taaaaaacgt
     5281 aatgaagtgt taactctccc ttgaagccta acaattggga gtgctgcctt gtatatttcc
     5341 ttctccgtta aataccgttt aaaaaacata attctccgcc tccgtatagc gtgcctattt
     5401 tatttcgtcg taaaacttag ctctgaaata ttcctttaga accatcatgt cattaataat
     5461 tgaatctatc tcatccattt tttcttggta ttcttttaat ggtgaaggga gcggtaactc
     5521 aatatttgaa aagtgattgg cgagataggg atcaaggttt ctcatccatt caggcttttc
     5581 gccaatgcgt tcttca
//
