LOCUS       NZ_CP054784            11970 bp    DNA     circular CON 15-MAY-2024
DEFINITION  Klebsiella pneumoniae strain KP20194a plasmid pKP20194a-p4, complete
            sequence.
ACCESSION   NZ_CP054784
VERSION     NZ_CP054784.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15186437
            Assembly: GCF_013349085.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11970)
  AUTHORS   Hu,L.M., Zhang,X.T., Zeng,X., Chen,Y., Liu,L. and Li,G.Q.
  TITLE     Genomic Characterization of a Carbapenemase-Producing, Extensively
            Drug-Resistant Klebsiella michiganensis Strain from a Renal Abscess
            Patient
  JOURNAL   Microbiol Resour Announc 11 (12), e0082522 (2022)
   PUBMED   36321904
REFERENCE   2  (bases 1 to 11970)
  AUTHORS   Zhang,X., Ouyang,J., He,W., Zeng,T., Liu,B., Jiang,H., Zhang,Y.,
            Zhou,L., Zhou,H., Liu,Z. and Liu,L.
  TITLE     Co-occurrence of Rapid Gene Gain and Loss in an Interhospital
            Outbreak of Carbapenem-Resistant Hypervirulent ST11-K64 Klebsiella
            pneumoniae
  JOURNAL   Front Microbiol 11, 579618 (2020)
   PUBMED   33281772
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 11970)
  AUTHORS   Zhang,X.T. and Liu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2020) Clinical Research institute, The Second
            Affiliated Hospital, University of South China, 35 Libration Ave.,
            Hengyang 421001, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 10-APR-2020
            Assembly Method                   :: SPAdes v. 3.14
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013349085.1-RS_2024_05_15
            Annotation Date                   :: 05/15/2024 02:38:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,916
            CDSs (total)                      :: 5,795
            Genes (coding)                    :: 5,617
            CDSs (with protein)               :: 5,617
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 67 of 178
            Pseudo Genes (incomplete)         :: 119 of 178
            Pseudo Genes (internal stop)      :: 24 of 178
            Pseudo Genes (multiple problems)  :: 32 of 178
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP054784.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11970
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP20194a"
                     /serotype="ST11-K64"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP20194a-p4"
                     /geo_loc_name="China: Hunan"
                     /lat_lon="27.69 N 111.66 E"
                     /collection_date="2019-04-03"
                     /collected_by="Xiaotuan Zhang"
     gene            complement(join(11253..11970,1..14))
                     /gene="mobC"
                     /locus_tag="HUR98_RS29260"
                     /old_locus_tag="HUR98_29265"
     CDS             complement(join(11253..11970,1..14))
                     /gene="mobC"
                     /locus_tag="HUR98_RS29260"
                     /old_locus_tag="HUR98_29265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(14..1978)
                     /locus_tag="HUR98_RS29265"
                     /old_locus_tag="HUR98_29270"
     CDS             complement(14..1978)
                     /locus_tag="HUR98_RS29265"
                     /old_locus_tag="HUR98_29270"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(3462..3611)
                     /locus_tag="HUR98_RS29270"
                     /old_locus_tag="HUR98_29275"
     CDS             complement(3462..3611)
                     /locus_tag="HUR98_RS29270"
                     /old_locus_tag="HUR98_29275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(3696..3953)
                     /locus_tag="HUR98_RS29275"
                     /old_locus_tag="HUR98_29280"
     CDS             complement(3696..3953)
                     /locus_tag="HUR98_RS29275"
                     /old_locus_tag="HUR98_29280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(3963..5648)
                     /locus_tag="HUR98_RS29280"
                     /old_locus_tag="HUR98_29285"
     CDS             complement(3963..5648)
                     /locus_tag="HUR98_RS29280"
                     /old_locus_tag="HUR98_29285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            complement(5753..7240)
                     /gene="ltrA"
                     /locus_tag="HUR98_RS29285"
                     /old_locus_tag="HUR98_29290"
     CDS             complement(5753..7240)
                     /gene="ltrA"
                     /locus_tag="HUR98_RS29285"
                     /old_locus_tag="HUR98_29290"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            7885..8130
                     /locus_tag="HUR98_RS29290"
                     /old_locus_tag="HUR98_29295"
     CDS             7885..8130
                     /locus_tag="HUR98_RS29290"
                     /old_locus_tag="HUR98_29295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            8404..8775
                     /gene="tolA"
                     /locus_tag="HUR98_RS29295"
                     /old_locus_tag="HUR98_29300"
     CDS             8404..8775
                     /gene="tolA"
                     /locus_tag="HUR98_RS29295"
                     /old_locus_tag="HUR98_29300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            8772..9137
                     /locus_tag="HUR98_RS29690"
     CDS             8772..9137
                     /locus_tag="HUR98_RS29690"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            9312..9590
                     /locus_tag="HUR98_RS29305"
                     /old_locus_tag="HUR98_29310"
     CDS             9312..9590
                     /locus_tag="HUR98_RS29305"
                     /old_locus_tag="HUR98_29310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            9578..9892
                     /locus_tag="HUR98_RS29310"
                     /old_locus_tag="HUR98_29315"
     CDS             9578..9892
                     /locus_tag="HUR98_RS29310"
                     /old_locus_tag="HUR98_29315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            10056..10484
                     /locus_tag="HUR98_RS29315"
                     /old_locus_tag="HUR98_29320"
     CDS             10056..10484
                     /locus_tag="HUR98_RS29315"
                     /old_locus_tag="HUR98_29320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            10510..10689
                     /locus_tag="HUR98_RS29320"
                     /old_locus_tag="HUR98_29325"
     CDS             10510..10689
                     /locus_tag="HUR98_RS29320"
                     /old_locus_tag="HUR98_29325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(10716..11246)
                     /locus_tag="HUR98_RS29325"
                     /old_locus_tag="HUR98_29330"
     CDS             complement(10716..11246)
                     /locus_tag="HUR98_RS29325"
                     /old_locus_tag="HUR98_29330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     BGC             1..10648
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          2781..3359
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(11257..11969)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.118"
                     /coverage="98.0"
                     /evalue="1.21e-138"
ORIGIN
        1 tgctccagtg tcatcagtaa agccccgcaa aatcatcatc cgcttttggt ccgccgccgt
       61 cggccggagg caggtactcc gccagcgggt ccgttttcgc cagcaccggg aaggttttca
      121 gcgaaatttc ccggcgctca acccgcagcg ggctgctgaa cgccagccgc gccacgccca
      181 gcccggtcat caccgcgaac ccgtcaggca ggtgttgcag ggtgttcacg tcaaacagcg
      241 gccgcgcctc cttctggatg aagctgtcgc cgctgatgtg ctccacgttc cccgcctccc
      301 ggctggcccg gcggcgctcc acgtccaccc ggatctcccc ggtctggccc gccgcccact
      361 gcgcggactc ctggctggcc gcgcgataga accagcggat cggcgtgtta tccagtaccg
      421 tggttttcac aaaatcagcg ggcagatcct gcccgcactg accgaagtcg cccagcgact
      481 ggtgcgcgag cagcaggttg cagtcccggc tgcgcagcgt ccccagcgcg ttcagcacgt
      541 acttcgacag cagatattta atctcatcga gcatgatgct cgcgtgcggc cacctccgga
      601 actcatcccg cgcaatgatg atttgcgcgc agcgggcaaa cagcatcttc tgcacccgga
      661 tcaccgcctc atcgtccatc gagccgatga cgtacaggca gccgccgccg ttgattatcc
      721 ccgccacatc gataccgctg tccgtctgca ggacgctcag gtcggccacg ttctccagct
      781 tggtgatcag ccccttcacg tcctttttca gggcctccgg caacgcgtac gccgccgcca
      841 gcacctgctg aatgtttgcc ccctgcggga actgctccgc aatcagcttc gccgctttct
      901 gctcgctgat gcggtagtgg tccgccacgt cgccggtctc actcaggtta aacccggcaa
      961 ccagcagatt cttcagggcg tactggtcga gatcgcggaa cagattcagc tgcgccggtt
     1021 tccccggccg cagatccagc agggtgaagt ccgggcagtg cgcccggaag acgtgcggcg
     1081 cccacgcgtc ccctttgggg tcgaagtaca ccacagcgtc cccgaaatcg cgtacgcagc
     1141 gaatgagcgc gttggtggcc atcgcgctct tgccgctccc gggcaggccg aggatctgaa
     1201 tattggtctt gcgccaggta tcacgaggga ccagcaccgg ctggccctgc tcatccttac
     1261 ccagaaacac cgcctgctgt ttccgggcct gctccgtgga aaaatcggcc agcgcgttaa
     1321 ccggtaccgg ctccccgaaa tgcaggtggc ggacgtctgt catttctccg gtccgctggc
     1381 tgctgcgccg gctctcctga ttcagtttcg cgttcaccgc ctcgtcagcg gccgcaaaat
     1441 gaccatcaag cagcaggaac gccagcagcc gcgccagaag gacgccggcg acaagccctc
     1501 cggcacacca ggctatcccg aacaggttag ccggctgcag caggtaataa tctgcgttcg
     1561 catagaggtc cgggcgcagg acggtttctc cccagctctc gataaacacg gcgacaacca
     1621 gcccccccag aagggccaga ccggcagcgc cggtcagcca gtacgcgtag ttccagacat
     1681 gtcgccgtgc cctgaccatc cgcccgggtg aatccagcga ggccagcacc gaaaatggca
     1741 cagtccggtt attatcgcgc ttatagcgat cggcatactc ccgccgcgcc ttatggctca
     1801 gccccacaaa acgcattcgg ccggcccaga gttcagccag accgaaaaac aggccgataa
     1861 ccagaaaaaa accggccaga ctgtaatact cccggtacag ccagctgacc cacgggtaaa
     1921 tctgccgggc cagctccggg atatgccact gccaggcaag cgaatccgta ttaaacatgc
     1981 aatctctctc cttcagacga cgggtttacc ggcgtgatgc gcagcccccg ctgcacaagc
     2041 cgcggacatg ccccaaggca tggcggcggc ggtcacgtcc gtggtgattg cgttcagctt
     2101 tcaccgcgga ggccagcctc tttccgcccc atgcggaaaa ggcgcagccc cggaggaaca
     2161 acgagcacgc cccagcgtgt gagaggaaaa ggcggcaccc cagtgcggtc ttttccggtg
     2221 ttccggcccc cgtcagagtt cgccatagcg agcggctgac cacccgcaaa atcaggagca
     2281 gctttgctgc ttcgtcaggt gcgtccagca ccgtggattt ttgtgggtgg tcaggcgtga
     2341 gcctgacggg ggccgggtga atttctggcg tggccggcgt ccagccggac agagccagaa
     2401 agaggggaaa agaccgtgcg tcggtggcgt ccagccagcg taagcaaggt cttttcccct
     2461 gaagtcagcc acgccccagc ggggatgact tgcatttctc cgcaaggaga aaaaagcaac
     2521 agagataggt taacgtaaaa tgttcagcga aaaaccgaca tggttcacct atcctgataa
     2581 ttgatcgtca gggaatagaa agacttaatc aggggacaat atcccacatc agcgcgtccg
     2641 gtgaccgtct tgcaggtgcc ggggaaattg cgcctcctcg ctcactcggt cgctacgctc
     2701 cgggcgtgag actgcggcgg gcgctgcgga cacatacaaa gttacccaca gattccgtgg
     2761 ataagcaggg gactaacatg tgaggcaaaa cggcagggcc gcgccggtgg cgtttttcca
     2821 taggctccgc cctcctgcca gagttcacat aaacagacgc tttttcggtg catctgtggg
     2881 agccgtgagg ctcaaccatg aatctgacag tacgggcgaa acccgacagg acttaaagat
     2941 ccccaccgtt tccggtaggt cgctccctct tgcgctctcc tgttccgacc ctgccgttta
     3001 ccggatacct gtccgccgtt ctcccttgcg ggaagtgtgg cgctttctca tagctcacac
     3061 actggtatct cggctcggtg taggtcgttc gctccaagct gggctgtaag caagaactcc
     3121 ccgttcagcc cgactgctgc gccttatccg gtaactgttc acttgagtcc aacccgggaa
     3181 agcacgcaaa acgccactgg cagcagccat tggtaactgg gagttcgcag aggatttgtt
     3241 tagctaaaca cgcggttgct cttgaagtgt gcgccaaagt ccggctacac tggaaggaca
     3301 gatttggttg ctgtgctctg cgaaagccag ttaccacggt taagcagttc cccaactgac
     3361 ttaaccttcg atcaaaccac ctccccaggt ggttttttcg tttacagggc aaaagattac
     3421 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctactgaac cgcgatcccc
     3481 gtcagttcag aggaggacga tggtgccacc gttccgccct gaacatcccg gatataattt
     3541 gcctgacatg cggccagaag aaagccagca acaatcaata taaaaagaaa aatagtcttt
     3601 acctttttca tatactttcc ctgtatttaa agactccggc attcatccct gaacaccgga
     3661 agtcagtatt tcaaatcatc cttgataatt ttttgttacc agttaccgtc ccgataatca
     3721 aaagctatat aacaattatc cacatcaggt tttatttgat ttttaaaaaa tggctgtagt
     3781 aaaggaaccc attctttctt caaatcaaac tcaccattat taatattatc ctttaaaggc
     3841 attcccaagc tttcaatgac agaaccatcg tcaccaaagt ctttagaata ctcttcccct
     3901 ttaaactctt cagttttctt atcaaaccat gtcaaacgta atttaagccc catatttacc
     3961 tctcaaagat attttttaat gttccgtttt ggatctggcc ctttaacctg cttacccgtt
     4021 ttgggatcaa atgcgcctag gtgttcgcca tcactggcac gatacccttc aagctcaccg
     4081 tgctgggaat cccattcata aattttacgt tttttatcgc cataccatcg ggcccgctta
     4141 ccaccaccac cttgtttagg tgttttagga tcacctcgct ttaactctcc aagtcccttt
     4201 atgtcttctg ttttaggtac tggatgataa tcatggccgt agtctttagt ccccttgcga
     4261 ggcttgttct tttcctcatt taacttattt tctgcatcct tcgctttttt ctccttttgt
     4321 ttccggctct ccatcgcccc ggtaagtgca gccttatctc tggataagga ttcattcatt
     4381 gcgtttaaag atgcctgagc cgcttttaat ttctcattct gattctgaat atccttatca
     4441 agatagcgaa cctgatcctg ccacccggag ccatattcat gaggcgggtt ataagtcaca
     4501 aaatcatctt tatttgcctc tgcctctttt acctttttat cggcttcctg aacagcagcc
     4561 cttgcaccgg ggagcgcctt ctctgtcgat gcaatgcggc tgtttaaggt cgcaatattt
     4621 ttatcttcgg catccagctc agctttcgct ttatcatagt ctcttttcag cccctcttcc
     4681 ggatgagcgg cgtcccatgc ctgctggcga cgcttttctt cctcctgacg ctgttttacc
     4741 tgtgccgggg taagaacatc tgtcaccgac acataaaccg gcttctgtcc ggtctctttc
     4801 gggaaacgaa taacggcctc acgggagtta ccaccggctg taaaaccagc cggacgtgaa
     4861 tcaccttttt cagcaacaat gccttttggc ggggctttcg ctgtcggaac acctttcggt
     4921 acgctcacct gcagagacgg gagacccgga atcgacacac tgaatacccc cggacgtttt
     4981 gtcggtttcg catccaccac agggacactc attggccgac ctgcgacaac cgcaatatgc
     5041 tgccgctcat ccttcaccac atccacaacc cgttgtctga cacgaaccgt cgcctggtcc
     5101 agtggtaaag tacttgccgg agtctccgtc accgcatcgg ccggtaatga cgtcataatt
     5161 tttgacatca ttttcggatc atcttttgca atctcagaag gcagcacacc gtagatcgca
     5221 atcccccaca gaccaaactt aaacggcccc ttcagggcag ccagcacatc agcaacggcg
     5281 gccgacaacg catcgccgga aactgataaa gccagtcctt cagcccccgg agtagccagg
     5341 gcaggcagac cgaaggccat cgcagtggcc gaagactgtc cgccaccaga gctaccgcta
     5401 tgattgccgc tgttaccacc attaccaccg ggtttactcc cgccgttacc aaactccccc
     5461 ggattatagt tatgaatatc tccattcggc gtatgcgtcg taccccagcc cggcccgctg
     5521 tttggatccg taccaccacc tgatgaaggg ccacctttac cagacgtacc gttcacattc
     5581 ccactggcct gaccaccatt atgtcccaga cctgaattac ccggacctcg accgtctcca
     5641 ccactcatac cgttttcctc tttgacattt aaaacaataa attaaagcca acatgtcgcg
     5701 gtagggatac ccgttaccgg ataccccccg cacagatccc ggcgtgcgcg atttacgcac
     5761 cgggctcctg cctcgggtgt ctggcggtga accgctccac aggccatgga tgaagaaccc
     5821 gaacccttgg tagccatgcg gctgccagtt tgtttgcttt cgtccaggtc gtatcatcct
     5881 tctggctcct gcgcctgagc gcccggcgcc agaggtttgt tacgtgtgtc ctgaacttct
     5941 gcatggtggg gaagttgccc ggtaccgagt gatagttcag gtatccctga accactctcc
     6001 tgagccattt tccctgttcg gggattgagt aatgccagcg ccttcgcaga ccgtctttga
     6061 tggctttcag agttgccgtc atccgatccc ggcgggtctt tcgtatcagc atgaacctgc
     6121 cgttgcgatc tttcccgctg atgtgcgtga acccgaggaa gttgaacgtt tctggtttgc
     6181 cttttcccct gatggcacgg ttttcggcag cgaagcggcc gaactccatc agacgggttt
     6241 tctccgggtg aaccgtgagt ccgaactccc tcagtctgcg ctgcatggct atacggaagc
     6301 gccgggcatc gtatcgtttg tcgaacccga tgacgatgtc atcggcgtat ctgaccatta
     6361 ccacattgcc tgtggcatag cgacgtcgcc actgatgcgc ccacagatcg aagacgtagt
     6421 ggaggtatat gtttgccagc agcggtgaga tgaccgcacc ctgtggggtg ccttcctccg
     6481 ttgctcgcca ttgaccctcc tccgacgtcc cggctgtgag ccacttacgt atgagcctga
     6541 ttaccctccg gtcgccgatc cgatgctctg tgaacctgat cagccattcg tggctcaccc
     6601 tgtcgaagaa ctgactgatg tcggcatcca gtacccagtt tacgttagtg cgtaccagcc
     6661 ctgtggccag tgcgtccagt gcatcgtgct ggcttcgccc gggtctgaac ccgtatgaga
     6721 accccataaa gtcgttttca tagactgcgt tcaggatttt caccagcgca tactggacga
     6781 tcttgtcctc cagcgaggcg atgccgagcg ggcgttgttt tccatccgct tttgggatgt
     6841 agtgacgcct gccgggctgc gccctgtagc tgccctgatg tagcctccgg tgcagatctg
     6901 ttatgttgtt cttcatgttt ccggcgtagt ccatccacct gatgccatcc actccggcgg
     6961 ccgctttcct gctcagggag aggaatgcgg cttccagtgc ttcgactgtc agcaggtgga
     7021 acaatgctgt aaaccgttct ttcttccgct gcttcgcagc ttcccgcacg cgtgacagcc
     7081 tctgtgacat gctttcccgg ctctgtgtcc ggcgcatgtg tggctgttcc gcgttcccct
     7141 tggccccgct ccttcgctcc actgactccg ctcctttcgg gttgttcgcc tgcttcgccg
     7201 ctactatgag cgagtccgac ttctcctctc cgtacatcac cggctatgac tcctcgtctt
     7261 cccggtgcgg gccatctccg acactggcag atggtcagag gggagatctc ccggttcccg
     7321 cgtagagatc gtattgacat gccagggtct cagaccccgc cgggtccatg tggcactcgc
     7381 agtgtcgcac cctatgatgt tgccttccgt taacagtaca acgtcggcac ccggtaattt
     7441 aatatacatt tcgtggctca atggctggcc tgtcaacacc cctgtcaacg cttcgcccca
     7501 tacctcgcgg tatgcaacgc atgactcggg gaccttgtgg attgctggtc cttcaatggt
     7561 cggggacttt caccccttga tctctaaccg gtctcccggc gcacactgta catatacaca
     7621 gtataattat taccttactc ctcccgtttt caggtgtcaa tatatgagat tagttctcat
     7681 cttcaggcac aaaaaagcgc ccggagccac ctgtaagcag atcgcgtctt ttcccgaaaa
     7741 tttctcatgg caacaaattt tattatttaa gacattcatc acacttacat ttcagatggt
     7801 atagacacat ctgaaagcta actggcatga ggagaggacc tgcggttaaa ctgttcggga
     7861 aacctgagac gcccggaggc cactatgacg cacctgaaac tgactcccgc tgaagaaaag
     7921 cgcctggcgg acgcctttgt cgctcaggca ctggccatgg gcctgaaagg gaacaaaacg
     7981 ctgccaaagg cggtaaaaca acaggtgcgg gaaagggtgc ttcgtgccat tcaggacgaa
     8041 cgtgaagcca ggcgggcgca ggaaatgcgt cgggagaaat acgaggctac ggaaaccacc
     8101 ttcacctggc agccacagcg ccgccggtaa ggggtattta ccataacgtc gcattgcccg
     8161 caccgctcag acggtctgag ggcgtttccg gcggctacgg gataaaacag gcaacaaacc
     8221 acccggaaaa ccgccggaaa cgcgcccggt aaattgtaac ccgctgcatg cctatgcacg
     8281 caactgcatg gcgggccgtt tggtgtaaaa acgatacgat aagccgtgtt tatgtaaatg
     8341 aacgtggccg taaagagtga caaatataca tgtaacattt tttactgaca ggtcatcaac
     8401 ccgatgcgtt taataccctt tatcctgctg gcattgtgca gccccttact cctgacggcc
     8461 tgtaagcatt ccaccaccca gccgccgaaa atttatactc tggataagtc atctaaaatc
     8521 agtagctacg ctgcggccat caagaccgct gttgaaatgc agatgtttga accagaggcc
     8581 tatgcgggaa aaatgtgcac cgtgcggata tccatacaac gtgatggcac tctgaacagc
     8641 gccattgctg aaggaggaga tccgaaactc tgtaaagcag caatatcagc cgttacccac
     8701 gccaaaattc cgccggcacc ggatgaaaat acctggcaaa tattcaaaaa cgcccctctg
     8761 gatttcagac catgaaagct tttactctga cagtgctggc ggccatgttc ctcactccgg
     8821 tacatgcgat accactaaaa cccgtaacca cactgcagcc aacctacccg gtacaggccg
     8881 cagcaaaacg catcgaaggt caggtgaccg ccacgtttga tattctggac gatggacgag
     8941 tggacaacgt gtcgcttacc ggctgtcacc ttttttttcg ggaaacgctg acggcaatga
     9001 ggcactggct atatcccgca gggcacccgg caatgcgaca ggttataaat tttgaattca
     9061 gtctgaaaaa tgacggctta caggatccgc gaccagtcac cacccaaaag ctcaatgatg
     9121 atggttgccg ccagtaacag gtcctttaac ccgcaggctg caatagtcct ggatgatgag
     9181 tgagcctgta gcagccctga ttagtggaag cgaagctgaa aaatgggcag caaaagccgt
     9241 atttctggca aatagcgccg ccacggatta aagtagtcat gtgtaataca tatgactaca
     9301 aaggggcttt tatgtcatca gcaacatcca tcaggctcga tgaagaactt aaggaccggc
     9361 tgaaaacgct ggccgatgac cggcaccgct cagcgcacgc gctaatgctg gaggcaatca
     9421 ccgaatacat cgaccgggaa gagaagcgca gccagtacct gcgggacgga caggccgcat
     9481 ggcagcacta tcaggaaacg ggtctgcacc ttactgcgga agaagcggaa gcctggataa
     9541 gtacctgggg aactgagaac gagcaggatg ctccgccatg ccacaggtaa cgatttccgc
     9601 gctggcacag cgcgatttac aacgccttca ggactttctc aaaaccaaaa atcggctggc
     9661 agccagaaaa ggaggtgagg tgatcgtccg ggctatccag caactgaaaa cattgccaga
     9721 cattggccgc ccggtgccgt ttctgccgct ggaatataag gaactggtga tcgggtttgg
     9781 cgacagtggc tatgtgatgc tctaccgcca cgaccgggaa atggaccaga ttgtgatcgt
     9841 caccgtcagg catcagaaag aatccgggta tccgggggca gacagcctct aataaccgat
     9901 gacgggggcg caattcattg cgtggccgtc gccggtttga caagcgcagc gcgtcagaaa
     9961 accacatcct gaacggaaaa acgggtaaaa ttcagcgata catttaccgt gagaaattac
    10021 acacctttca cacccgataa aatcaggggt atacagtggc gaaaacattg ctcaaccttt
    10081 cacaggcagc acaagccgcg ggcattacca gaagaacgct atacaatcat gtaaaacagg
    10141 gaaaggttac cgtgtcacgt gacgggaaaa acaaccctgt cgtggatgtg tcagaactga
    10201 tccgtgtata cggaaatgtg aatatccctg agaaacaaat tcccggcatt tcacaccgtg
    10261 aaaatacaca aaaaaatttc ccacaggaac agcttctggc aatgcaaaag gaactggccg
    10321 atctccggca ggctgtcacc ctgatgctgg aagataaaac atcccgggaa gaagaacgcc
    10381 gccagcacga tgatgagcgc cggaaactcc aggccgaagt ggacagattg acaacagaac
    10441 tgacacagaa gaaaaaacgc ttctggtccg gctggtttag ctgagaactt accgaaggga
    10501 atggagaaca tgacgaagca ggaaaaaact gccctcaata tggctaaatt cattaaggat
    10561 cagtcgctgc tgttgctgga aaagctgaac gagctggaca tggatacccc ggccgacatg
    10621 tgcgaacggc ttcacgagga tgcggaaaca ctctggcagg tgatgcggga taagctcggg
    10681 gaagaataac agcaccggcc atgccggtgc tggcgttatt ttttgccgaa cggccaccac
    10741 cggcgggcgg gtttgtcctc cggcagttca ggcaccggca cgttaaaata gcggctgtta
    10801 tattcccgca tcagctccgc tttcagcttc tcgttcaccg cgctttcccc gtgccgctcg
    10861 ctgaccacct caatcatcgc gttaatctgc tccggtgagc acttcccggc atcaaccacc
    10921 tgcacaccgt tctcgatggc gaaccgctcc acctttcgat agaggtaatc acacagcttc
    10981 attcctgtgg gtttgtcccg ttcccattgc ggattttcac cggtgaaaaa gtacgtggct
    11041 ttttcgtcgc tgttgagccg ctcgtcatac atcccgatgg atttcatcat cgccagatgc
    11101 tgggcccggt cgacaaaaca gaaaacatac gccccggtct gccaggcgag caccaccatg
    11161 tcttccagat gctcgctggt tggcataatg cagcgctcgc cgtctttgtc ttccacgtcc
    11221 tcaataaata ccatgtacag cgccataatc cctcactccg ttgtcataaa tttaaagcac
    11281 gcgaacagcc tctcccggtc cagctccacc gttttgcggg cctcgccggt ctgtggattc
    11341 acgatttcat ccaccgccag aatcgcccgc tgtacccgga caagcgtcgg ccggtcaggg
    11401 cagatgtacc agatttcatc gatgccgttc gcccggcggt tgaacagata cccggacacc
    11461 acttcctgat accgcctgcg gctcttgacg gtgcgctcta cctcaaatgc caccgttctg
    11521 ccgtccagct ccgccagcgc gtccggcact ttcatccctt tcagggccat acggtgcagg
    11581 taccgccagc cggtggcccc ccggccctcc atggagaggc gcagggactg cacggccagc
    11641 cggtgcggca gctgcgccgc cgacaccctt gacggctgaa acggaataac gtccgtcagc
    11701 ggctcgtcct cgtcgaacga gaacgccacc ccgtgcggcg tcagccccca cacgctgacc
    11761 tgacggccgt caaccgggta tttcgcccgg accagcagcc ctttcgccac cagactgtcc
    11821 aggaagcggt aaaccggctg taccgccgca atccccagcc gtcgcgcaac cagctccgcc
    11881 gtggaacaca gctcttcccg cagccagcgc agggtcagcc gtgttttctc cgcgttccgc
    11941 gcctgccgct tcgcgtggct gacgttgtac
//
