LOCUS       NZ_CP054767             5596 bp    DNA     circular CON 16-MAY-2024
DEFINITION  Klebsiella pneumoniae strain KP20194b2 plasmid pKP20194b2-p5,
            complete sequence.
ACCESSION   NZ_CP054767
VERSION     NZ_CP054767.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15186440
            Assembly: GCF_013348885.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Hu,L.M., Zhang,X.T., Zeng,X., Chen,Y., Liu,L. and Li,G.Q.
  TITLE     Genomic Characterization of a Carbapenemase-Producing, Extensively
            Drug-Resistant Klebsiella michiganensis Strain from a Renal Abscess
            Patient
  JOURNAL   Microbiol Resour Announc 11 (12), e0082522 (2022)
   PUBMED   36321904
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Zhang,X., Ouyang,J., He,W., Zeng,T., Liu,B., Jiang,H., Zhang,Y.,
            Zhou,L., Zhou,H., Liu,Z. and Liu,L.
  TITLE     Co-occurrence of Rapid Gene Gain and Loss in an Interhospital
            Outbreak of Carbapenem-Resistant Hypervirulent ST11-K64 Klebsiella
            pneumoniae
  JOURNAL   Front Microbiol 11, 579618 (2020)
   PUBMED   33281772
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5596)
  AUTHORS   Zhang,X.T. and Liu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2020) Clinical Research institute, The Second
            Affiliated Hospital, University of South China, 35 Libration Ave.,
            Hengyang 421001, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 10-APR-2020
            Assembly Method                   :: SPAdes v. 3.14
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013348885.1-RS_2024_05_16
            Annotation Date                   :: 05/16/2024 02:55:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,918
            CDSs (total)                      :: 5,796
            Genes (coding)                    :: 5,618
            CDSs (with protein)               :: 5,618
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 67 of 178
            Pseudo Genes (incomplete)         :: 119 of 178
            Pseudo Genes (internal stop)      :: 24 of 178
            Pseudo Genes (multiple problems)  :: 32 of 178
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP054767.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP20194b2"
                     /serotype="ST11-K64"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP20194b2-p5"
                     /geo_loc_name="China: Hunan"
                     /lat_lon="27.69 N 111.66 E"
                     /collection_date="2019-04-12"
                     /collected_by="Xiaotuan Zhang"
     gene            complement(join(5452..5596,1..401))
                     /locus_tag="HUS01_RS29335"
                     /old_locus_tag="HUS01_29340"
     CDS             complement(join(5452..5596,1..401))
                     /locus_tag="HUS01_RS29335"
                     /old_locus_tag="HUS01_29340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(427..753)
                     /locus_tag="HUS01_RS29340"
                     /old_locus_tag="HUS01_29345"
     CDS             complement(427..753)
                     /locus_tag="HUS01_RS29340"
                     /old_locus_tag="HUS01_29345"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            complement(1111..1269)
                     /locus_tag="HUS01_RS29930"
     CDS             complement(1111..1269)
                     /locus_tag="HUS01_RS29930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            1909..2085
                     /locus_tag="HUS01_RS29345"
                     /old_locus_tag="HUS01_29350"
     CDS             1909..2085
                     /locus_tag="HUS01_RS29345"
                     /old_locus_tag="HUS01_29350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            2075..2419
                     /locus_tag="HUS01_RS29350"
                     /old_locus_tag="HUS01_29355"
     CDS             2075..2419
                     /locus_tag="HUS01_RS29350"
                     /old_locus_tag="HUS01_29355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2628..>3062
                     /locus_tag="HUS01_RS29755"
                     /pseudo=""
     CDS             2628..>3062
                     /locus_tag="HUS01_RS29755"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3097..3606
                     /locus_tag="HUS01_RS29360"
                     /old_locus_tag="HUS01_29365"
     CDS             3097..3606
                     /locus_tag="HUS01_RS29360"
                     /old_locus_tag="HUS01_29365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            3613..3825
                     /locus_tag="HUS01_RS29365"
                     /old_locus_tag="HUS01_29370"
     CDS             3613..3825
                     /locus_tag="HUS01_RS29365"
                     /old_locus_tag="HUS01_29370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            4492..4716
                     /locus_tag="HUS01_RS29370"
                     /old_locus_tag="HUS01_29375"
     CDS             4492..4716
                     /locus_tag="HUS01_RS29370"
                     /old_locus_tag="HUS01_29375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            4700..4966
                     /locus_tag="HUS01_RS29375"
                     /old_locus_tag="HUS01_29380"
     CDS             4700..4966
                     /locus_tag="HUS01_RS29375"
                     /old_locus_tag="HUS01_29380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(5036..5386)
                     /locus_tag="HUS01_RS29380"
                     /old_locus_tag="HUS01_29385"
     CDS             complement(5036..5386)
                     /locus_tag="HUS01_RS29380"
                     /old_locus_tag="HUS01_29385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     Typing          1744..1823
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(1161..1558)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          2410..3941
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 taacctgagt taagggtttc ttttatactt tcttcaatgc tcattataaa tattagtgga
       61 ttatcatttt tcttccccca tgggtttcta attaatcctt cgatgtactc aggtcctttg
      121 cgtcctattg cctgcgcaat attttttatt tcatctgcgt gagggctttg ttgtacagct
      181 ttgtttatgc ctgcttgagt gattttcaga gactctacac cgtgtatgtt gtatgagata
      241 aaatgtaata aattaagatg caatgcaatt aatccatttt tgtttacatc tgagtatttg
      301 gataaaaaac gtaatgaagt gttaactctc ccttgaagcc taacaattgg gagtgctgcc
      361 ttgtatattt ccttctccgt taaataccgt ttaaaaaaca taattctccg cctccgtata
      421 gcgtgcctat tttatttcgt cgtaaaactt agctctgaaa tattccttta gaaccatcat
      481 gtcattaata attgaatcta tctcatccat tttttcttgg tattctttta atggtgaagg
      541 gagcggtaac tcaatatttg aaaagtgatt ggcgagatag ggatcaaggt ttctcatcca
      601 ttcaggcttt tcgccaatgc gttcttcaac tgaaagtgaa cctgataatc tctggttaat
      661 aagatcactt agactcattt tggttttatt cgaaagccgt tgtaaggatt gctttaatcc
      721 tatatctagc atgatatttg ttctttctct cattaccccc ccttgctatt gcgttttttg
      781 ttgcgcacaa agctaccatt gcgcattaag aaacgcaata aaaaaggagg gagggagtgg
      841 tttgtacatg atgtgcagtg cgctgagggc atcagtggcg ggtgtttttc cgtgggatgg
      901 atggagctcc ggggataaca gaactgacga tcatttaaag gatcttcttg agatcctttt
      961 tttctgcgcg taatctcttg cactgtaaac gaaaaaaccg cctagggagg cggtttgatc
     1021 gaaggttaag tcagttgggg aactgcttaa ccgggtaact gggtatacga gaccgcagat
     1081 accaaaactg ttctttcagt gtagccgcag ttaggcaccc acttcaagac ctctcaatat
     1141 ctctctcgta tatatccagt taccaatggc tgctgccagt ggcgtttttg cgtgtccgtc
     1201 cgggttggac tcaagatgat agttaccgga aagggcgcag cagtcgggct gaacgggggg
     1261 ttcgtgcata cagcccagct tggagcgaac gacctaaacc gagccgagat accagcagcg
     1321 tgagctatga gaaagcgcca cgcttcccga aagggaaaaa ggcggaacag gtatccggta
     1381 aacggcaggg tcggaacagg agagcgcacg agggagccgc cggagagaaa tcaccggcat
     1441 ctttaagtcc tgtcgggttt cgtcctcctc tgacttgagc gtccatttct gtgatgctcg
     1501 tcaggggagg cggagcctat ggaaaaacgg caacgccgca tccttttctc cggccctttc
     1561 tgatttgagc gcctttgctt tatcccgtta tccgtgagtc ggtcacgccc gccgcagccg
     1621 aacggcagga gcgcagctac cgagtgagcg aggaggcgtc atttcatctg tccgtgcatg
     1681 gtattgcgga cgctcctgag ccacatatca cacctgccac atgaggcact ttcatgaatc
     1741 gtgctctcat gccaacatag taagccagta tacacagcgt aaacgctgtg actggttcag
     1801 ggctgcgccc cgaaacccgc taacaccact gacgcgctgg cgcttgtcca acgccggagc
     1861 gacccggaaa acttttccgt gccgtccggc gtttgcgaag gagggataat gcagaagccg
     1921 aagaagaagc cgctcgtcgt aaaaaaaaga tcctggcatc cggcagtgat ggcaaatctg
     1981 atgcgtcagg cacaggagga agattaccgc cgggagcaga tggcagagct tgagcgttca
     2041 gcccggtcac attttgcgga tatcggagga gaggatggcg gttaagcgta cccgtttttt
     2101 aggtatccgg gtgaccgatg gggagtacca gcagctgctg gagcgctgca atggaaggca
     2161 actggcggtg tggatgcggg aaacctgtct cgatacgcgc ccggcgcggt cgttgcggct
     2221 gccgtccatc gatccggttt tgctgcgcca gcttgccggg atggggaaca acctcaacca
     2281 gatcgcccgg aaaattaacg gcggccagtg gtcaggcgct gaccgggttc aggtggtggc
     2341 cgcgctgatg gccattgatg ccgaactcga gcggctgcgg catgccgtgc tggaaaaggg
     2401 ggcagacgat gatcgttaag tttcatccgc gagggcgagg cggcggtgcc gggccggtgg
     2461 attatctgct ggggaaagac cgccagcgcg acggggccat cgtcctgcag ggaaagccgg
     2521 aggaggtccg ggagcttatc gacgcctcgc cctacgccaa aaagtacacc tccggcgtcc
     2581 tgtcttttgc tgaacaggat ttaccgcccg gccagcgtga aaagctgatg gcgagcttcg
     2641 agcgggttct gatgcccgga ctcgataaag accagtacag cgtgctgtgg gttgaacacc
     2701 gggacaaggg gcggctggaa ctgaattttc tggtaccgaa cacggaactg ctgaccggca
     2761 ggcgtctcca gccgtactac gaccgggcag accgtccgcg catcgatgcg tggcagaccg
     2821 tggtgaacgg gcgactgggg ctgcacgacc cgaacgcacc ggagaaccgg cgcgcgctgg
     2881 tgacgccatc ttcgttaccc aaaacgaagc aggaagccgc agaggctatt acgcggggct
     2941 tactggccct cgcctcgtca ggggagctta aaacgcgtca ggacgtcact gaggcgctgg
     3001 aaagcgcagg ttttgaggtc gtgcgcacca caaagagcag catcagcatt gccgacccgg
     3061 acggagggcg aaacatccga cttaagggag ccatctatga acagtctttt aacgctggcg
     3121 aaggacttag agcagaaatc gaaagcgcag cagcagagta ccggcgaaat gctgaaagcc
     3181 gcattcagcg agcacgagaa gtctgtcaga gcggaactga gcgaaagcgg gaagagaatc
     3241 agcgccgcca tccgcgccca cgagcagggt atgagcgcgg ccatgcagtc gaaccgcctg
     3301 agcgtgatgc gtatggtcgg gcggacatgg ctgaccatca cgatggtgtc aggactgctg
     3361 ttcgccagcc tgagcggggt gttgtggtat caggggagct tgatagcgtc aaatctggcc
     3421 gaaatcgacc ggcagaacgc agcgctgtcg aagctgaacg cgaagacctg gggcgtgacg
     3481 tacctggaag acagcaacgg acgttttctg gtgctgccga aggggacggc cgtagacagg
     3541 acgcagagct ggacggtcgg gaacgggcgg tcgaagcaga acgcgctgag actggtgaag
     3601 gagtaagtgc cgatgaccga gctggaaaaa cagttgctga gcgcattaga gcagctacag
     3661 caggactact cgaaaaggct ggacgagtgg gagagcgcct tcggggaatg gcggaaaatg
     3721 tcagggctta tgcaacggga gaacgcggcg ctgagcgagc gcgtgacgcg cttgagtcag
     3781 caggtggaac gcttgagcgg gcaactgcag cgtttgagcc ggtagtgcag cgtcatgaga
     3841 tggccgtggc cgcagaacgg gcgcacgagc aacggcagca cgaaaaggaa ctgacggaag
     3901 cgcgttcccg gcagcgaagc tatgacggtc cgtcgctggg ctgaaatgca ggaaaacagg
     3961 gttctcatcg ggcatttacg ctgaaaccgg atgtcattca cctggctgca tggctatgca
     4021 gccaggtaaa atttatctgt cgcgaacggg gcggtttatc gggtggtttg ttgccggttt
     4081 tacctgactg ccaccccgtt cgcggcgaac ccgtccgggg cggtgcgggc aacgggtgtt
     4141 atgtttaatc agcgtgaacg gcagatacaa aaaagccccg gagggctttt aactactgac
     4201 ttattttttt gaagatttgc tgggtttcaa tttgtcagta tttcccggcg tttttttatt
     4261 gtcacgctgg cgtttatcag gttcccctgt tgaatcggca attcgtttcg gacccggctt
     4321 aagacccata atcgttatcc tttcaatagt taagaggaaa gccctcaagc tagatatatt
     4381 gaccgcgtta cggattatca agaggggcta acaagattat tttttgttct tttaggttga
     4441 ttgtggtact gttgatatac atgtatagca aatggtgttc gggaggttta tatgcttgct
     4501 atccggttat ctgatgagat tgagtcccgt ctggactcgc tggcgaagca aaccggcaga
     4561 acaaagacgt tttatgcgcg ggaagcaata ctggcgcatc tggaagacct ggaggattat
     4621 tatctttcag cagaaactgc tgcacgcgtt cgccgtggtg atgaagcagt gcattcgtct
     4681 gaagacgtga ggaagtcact tggtctggac gattaactat tccgatcggg cgctcaaatc
     4741 gttacgcaag atggacaaac agaacgcacg acggattgtg gattttatga gtttacgcat
     4801 tgcagttgct gccgatcctc gccagtcagg gaagccgctc aaaggtgagc tgggcgagtt
     4861 ctggcgctat cgggtgggag attatcgcgt tctgtgtgag atccgagatg acgagcttgt
     4921 tatccttgcc gccacgattg gacatcgccg cgaagtttac gactgaagcc cgctatgcgg
     4981 gcttttttga tgccgacaac aggtattatg gtaaatactc gactaactac gtgttctact
     5041 gtttgtcggt tttagttaac cagcaggttg tcttaacgat aatgtagccg ttatttttaa
     5101 ctaataaggt catattgtta acgtcaggca taataaaaga cattatggcc tcagagttat
     5161 catcagaagc agctctctgc attactactt cgattccctt ttctttgaat ctataagtat
     5221 ccagttgcct attaaactta gaaagaccta caaccatact tagtttgtta tctttaacag
     5281 taagagatgc ttcctgagta ccattacatc ggtagtaagc ggtgtaaccg tcagcaaaag
     5341 atcgaccggg gaatataaat gctaggaacg caattaacca atacatagtt gagaatcttg
     5401 gtattacaca tttcactatc ctctctttca tttttcgttc accatacttt gttatctgat
     5461 ttttaatttt tctattttat aaaccaagtc tctaccaaaa aggcgaaaat tatcctcaaa
     5521 taataataat tgtttaagaa catccatgaa agcagctgct tcaccctcag ttatgggagc
     5581 tactctatat aacata
//
