LOCUS       NZ_CP054766            11970 bp    DNA     circular CON 16-MAY-2024
DEFINITION  Klebsiella pneumoniae strain KP20194b2 plasmid pKP20194b2-p4,
            complete sequence.
ACCESSION   NZ_CP054766
VERSION     NZ_CP054766.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15186440
            Assembly: GCF_013348885.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11970)
  AUTHORS   Hu,L.M., Zhang,X.T., Zeng,X., Chen,Y., Liu,L. and Li,G.Q.
  TITLE     Genomic Characterization of a Carbapenemase-Producing, Extensively
            Drug-Resistant Klebsiella michiganensis Strain from a Renal Abscess
            Patient
  JOURNAL   Microbiol Resour Announc 11 (12), e0082522 (2022)
   PUBMED   36321904
REFERENCE   2  (bases 1 to 11970)
  AUTHORS   Zhang,X., Ouyang,J., He,W., Zeng,T., Liu,B., Jiang,H., Zhang,Y.,
            Zhou,L., Zhou,H., Liu,Z. and Liu,L.
  TITLE     Co-occurrence of Rapid Gene Gain and Loss in an Interhospital
            Outbreak of Carbapenem-Resistant Hypervirulent ST11-K64 Klebsiella
            pneumoniae
  JOURNAL   Front Microbiol 11, 579618 (2020)
   PUBMED   33281772
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 11970)
  AUTHORS   Zhang,X.T. and Liu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2020) Clinical Research institute, The Second
            Affiliated Hospital, University of South China, 35 Libration Ave.,
            Hengyang 421001, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 10-APR-2020
            Assembly Method                   :: SPAdes v. 3.14
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013348885.1-RS_2024_05_16
            Annotation Date                   :: 05/16/2024 02:55:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,918
            CDSs (total)                      :: 5,796
            Genes (coding)                    :: 5,618
            CDSs (with protein)               :: 5,618
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 67 of 178
            Pseudo Genes (incomplete)         :: 119 of 178
            Pseudo Genes (internal stop)      :: 24 of 178
            Pseudo Genes (multiple problems)  :: 32 of 178
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP054766.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11970
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP20194b2"
                     /serotype="ST11-K64"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP20194b2-p4"
                     /geo_loc_name="China: Hunan"
                     /lat_lon="27.69 N 111.66 E"
                     /collection_date="2019-04-12"
                     /collected_by="Xiaotuan Zhang"
     gene            complement(join(11953..11970,1..348))
                     /locus_tag="HUS01_RS29750"
     CDS             complement(join(11953..11970,1..348))
                     /locus_tag="HUS01_RS29750"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(345..716)
                     /gene="tolA"
                     /locus_tag="HUS01_RS29270"
                     /old_locus_tag="HUS01_29275"
     CDS             complement(345..716)
                     /gene="tolA"
                     /locus_tag="HUS01_RS29270"
                     /old_locus_tag="HUS01_29275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(990..1235)
                     /locus_tag="HUS01_RS29275"
                     /old_locus_tag="HUS01_29280"
     CDS             complement(990..1235)
                     /locus_tag="HUS01_RS29275"
                     /old_locus_tag="HUS01_29280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            1880..3367
                     /gene="ltrA"
                     /locus_tag="HUS01_RS29280"
                     /old_locus_tag="HUS01_29285"
     CDS             1880..3367
                     /gene="ltrA"
                     /locus_tag="HUS01_RS29280"
                     /old_locus_tag="HUS01_29285"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            3472..5157
                     /locus_tag="HUS01_RS29285"
                     /old_locus_tag="HUS01_29290"
     CDS             3472..5157
                     /locus_tag="HUS01_RS29285"
                     /old_locus_tag="HUS01_29290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            5167..5424
                     /locus_tag="HUS01_RS29290"
                     /old_locus_tag="HUS01_29295"
     CDS             5167..5424
                     /locus_tag="HUS01_RS29290"
                     /old_locus_tag="HUS01_29295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            5509..5658
                     /locus_tag="HUS01_RS29295"
                     /old_locus_tag="HUS01_29300"
     CDS             5509..5658
                     /locus_tag="HUS01_RS29295"
                     /old_locus_tag="HUS01_29300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            7142..9106
                     /locus_tag="HUS01_RS29300"
                     /old_locus_tag="HUS01_29305"
     CDS             7142..9106
                     /locus_tag="HUS01_RS29300"
                     /old_locus_tag="HUS01_29305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            9106..9837
                     /gene="mobC"
                     /locus_tag="HUS01_RS29305"
                     /old_locus_tag="HUS01_29310"
     CDS             9106..9837
                     /gene="mobC"
                     /locus_tag="HUS01_RS29305"
                     /old_locus_tag="HUS01_29310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            9844..10374
                     /locus_tag="HUS01_RS29310"
                     /old_locus_tag="HUS01_29315"
     CDS             9844..10374
                     /locus_tag="HUS01_RS29310"
                     /old_locus_tag="HUS01_29315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(10401..10580)
                     /locus_tag="HUS01_RS29315"
                     /old_locus_tag="HUS01_29320"
     CDS             complement(10401..10580)
                     /locus_tag="HUS01_RS29315"
                     /old_locus_tag="HUS01_29320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(10606..11034)
                     /locus_tag="HUS01_RS29320"
                     /old_locus_tag="HUS01_29325"
     CDS             complement(10606..11034)
                     /locus_tag="HUS01_RS29320"
                     /old_locus_tag="HUS01_29325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(11198..11512)
                     /locus_tag="HUS01_RS29325"
                     /old_locus_tag="HUS01_29330"
     CDS             complement(11198..11512)
                     /locus_tag="HUS01_RS29325"
                     /old_locus_tag="HUS01_29330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(11500..11778)
                     /locus_tag="HUS01_RS29330"
                     /old_locus_tag="HUS01_29335"
     CDS             complement(11500..11778)
                     /locus_tag="HUS01_RS29330"
                     /old_locus_tag="HUS01_29335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     BGC             1..10157
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(5762..6340)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          9107..9834
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 atcattgagc ttttgggtgg tgactggtcg cggatcctgt aagccgtcat ttttcagact
       61 gaattcaaaa tttataacct gtcgcattgc cgggtgccct gcgggatata gccagtgcct
      121 cattgccgtc agcgtttccc gaaaaaaaag gtgacagccg gtaagcgaca cgttgtccac
      181 tcgtccatcg tccagaatat caaacgtggc ggtcacctga ccttcgatgc gttttgctgc
      241 ggcctgtacc gggtaggttg gctgcagtgt ggttacgggt tttagtggta tcgcatgtac
      301 cggagtgagg aacatggccg ccagcactgt cagagtaaaa gctttcatgg tctgaaatcc
      361 agaggggcgt ttttgaatat ttgccaggta ttttcatccg gtgccggcgg aattttggcg
      421 tgggtaacgg ctgatattgc tgctttacag agtttcggat ctcctccttc agcaatggcg
      481 ctgttcagag tgccatcacg ttgtatggat atccgcacgg tgcacatttt tcccgcatag
      541 gcctctggtt caaacatctg catttcaaca gcggtcttga tggccgcagc gtagctactg
      601 attttagatg acttatccag agtataaatt ttcggcggct gggtggtgga atgcttacag
      661 gccgtcagga gtaaggggct gcacaatgcc agcaggataa agggtattaa acgcatcggg
      721 ttgatgacct gtcagtaaaa aatgttacat gtatatttgt cactctttac ggccacgttc
      781 atttacataa acacggctta tcgtatcgtt tttacaccaa acggcccgcc atgcagttgc
      841 gtgcataggc atgcagcggg ttacaattta ccgggcgcgt ttccggcggt tttccgggtg
      901 gtttgttgcc tgttttatcc cgtagccgcc ggaaacgccc tcagaccgtc tgagcggtgc
      961 gggcaatgcg acgttatggt aaatacccct taccggcggc gctgtggctg ccaggtgaag
     1021 gtggtttccg tagcctcgta tttctcccga cgcatttcct gcgcccgcct ggcttcacgt
     1081 tcgtcctgaa tggcacgaag caccctttcc cgcacctgtt gttttaccgc ctttggcagc
     1141 gttttgttcc ctttcaggcc catggccagt gcctgagcga caaaggcgtc cgccaggcgc
     1201 ttttcttcag cgggagtcag tttcaggtgc gtcatagtgg cctccgggcg tctcaggttt
     1261 cccgaacagt ttaaccgcag gtcctctcct catgccagtt agctttcaga tgtgtctata
     1321 ccatctgaaa tgtaagtgtg atgaatgtct taaataataa aatttgttgc catgagaaat
     1381 tttcgggaaa agacgcgatc tgcttacagg tggctccggg cgcttttttg tgcctgaaga
     1441 tgagaactaa tctcatatat tgacacctga aaacgggagg agtaaggtaa taattatact
     1501 gtgtatatgt acagtgtgcg ccgggagacc ggttagagat caaggggtga aagtccccga
     1561 ccattgaagg accagcaatc cacaaggtcc ccgagtcatg cgttgcatac cgcgaggtat
     1621 ggggcgaagc gttgacaggg gtgttgacag gccagccatt gagccacgaa atgtatatta
     1681 aattaccggg tgccgacgtt gtactgttaa cggaaggcaa catcataggg tgcgacactg
     1741 cgagtgccac atggacccgg cggggtctga gaccctggca tgtcaatacg atctctacgc
     1801 gggaaccggg agatctcccc tctgaccatc tgccagtgtc ggagatggcc cgcaccggga
     1861 agacgaggag tcatagccgg tgatgtacgg agaggagaag tcggactcgc tcatagtagc
     1921 ggcgaagcag gcgaacaacc cgaaaggagc ggagtcagtg gagcgaagga gcggggccaa
     1981 ggggaacgcg gaacagccac acatgcgccg gacacagagc cgggaaagca tgtcacagag
     2041 gctgtcacgc gtgcgggaag ctgcgaagca gcggaagaaa gaacggttta cagcattgtt
     2101 ccacctgctg acagtcgaag cactggaagc cgcattcctc tccctgagca ggaaagcggc
     2161 cgccggagtg gatggcatca ggtggatgga ctacgccgga aacatgaaga acaacataac
     2221 agatctgcac cggaggctac atcagggcag ctacagggcg cagcccggca ggcgtcacta
     2281 catcccaaaa gcggatggaa aacaacgccc gctcggcatc gcctcgctgg aggacaagat
     2341 cgtccagtat gcgctggtga aaatcctgaa cgcagtctat gaaaacgact ttatggggtt
     2401 ctcatacggg ttcagacccg ggcgaagcca gcacgatgca ctggacgcac tggccacagg
     2461 gctggtacgc actaacgtaa actgggtact ggatgccgac atcagtcagt tcttcgacag
     2521 ggtgagccac gaatggctga tcaggttcac agagcatcgg atcggcgacc ggagggtaat
     2581 caggctcata cgtaagtggc tcacagccgg gacgtcggag gagggtcaat ggcgagcaac
     2641 ggaggaaggc accccacagg gtgcggtcat ctcaccgctg ctggcaaaca tatacctcca
     2701 ctacgtcttc gatctgtggg cgcatcagtg gcgacgtcgc tatgccacag gcaatgtggt
     2761 aatggtcaga tacgccgatg acatcgtcat cgggttcgac aaacgatacg atgcccggcg
     2821 cttccgtata gccatgcagc gcagactgag ggagttcgga ctcacggttc acccggagaa
     2881 aacccgtctg atggagttcg gccgcttcgc tgccgaaaac cgtgccatca ggggaaaagg
     2941 caaaccagaa acgttcaact tcctcgggtt cacgcacatc agcgggaaag atcgcaacgg
     3001 caggttcatg ctgatacgaa agacccgccg ggatcggatg acggcaactc tgaaagccat
     3061 caaagacggt ctgcgaaggc gctggcatta ctcaatcccc gaacagggaa aatggctcag
     3121 gagagtggtt cagggatacc tgaactatca ctcggtaccg ggcaacttcc ccaccatgca
     3181 gaagttcagg acacacgtaa caaacctctg gcgccgggcg ctcaggcgca ggagccagaa
     3241 ggatgatacg acctggacga aagcaaacaa actggcagcc gcatggctac caagggttcg
     3301 ggttcttcat ccatggcctg tggagcggtt caccgccaga cacccgaggc aggagcccgg
     3361 tgcgtaaatc gcgcacgccg ggatctgtgc ggggggtatc cggtaacggg tatccctacc
     3421 gcgacatgtt ggctttaatt tattgtttta aatgtcaaag aggaaaacgg tatgagtggt
     3481 ggagacggtc gaggtccggg taattcaggt ctgggacata atggtggtca ggccagtggg
     3541 aatgtgaacg gtacgtctgg taaaggtggc ccttcatcag gtggtggtac ggatccaaac
     3601 agcgggccgg gctggggtac gacgcatacg ccgaatggag atattcataa ctataatccg
     3661 ggggagtttg gtaacggcgg gagtaaaccc ggtggtaatg gtggtaacag cggcaatcat
     3721 agcggtagct ctggtggcgg acagtcttcg gccactgcga tggccttcgg tctgcctgcc
     3781 ctggctactc cgggggctga aggactggct ttatcagttt ccggcgatgc gttgtcggcc
     3841 gccgttgctg atgtgctggc tgccctgaag gggccgttta agtttggtct gtgggggatt
     3901 gcgatctacg gtgtgctgcc ttctgagatt gcaaaagatg atccgaaaat gatgtcaaaa
     3961 attatgacgt cattaccggc cgatgcggtg acggagactc cggcaagtac tttaccactg
     4021 gaccaggcga cggttcgtgt cagacaacgg gttgtggatg tggtgaagga tgagcggcag
     4081 catattgcgg ttgtcgcagg tcggccaatg agtgtccctg tggtggatgc gaaaccgaca
     4141 aaacgtccgg gggtattcag tgtgtcgatt ccgggtctcc cgtctctgca ggtgagcgta
     4201 ccgaaaggtg ttccgacagc gaaagccccg ccaaaaggca ttgttgctga aaaaggtgat
     4261 tcacgtccgg ctggttttac agccggtggt aactcccgtg aggccgttat tcgtttcccg
     4321 aaagagaccg gacagaagcc ggtttatgtg tcggtgacag atgttcttac cccggcacag
     4381 gtaaaacagc gtcaggagga agaaaagcgt cgccagcagg catgggacgc cgctcatccg
     4441 gaagaggggc tgaaaagaga ctatgataaa gcgaaagctg agctggatgc cgaagataaa
     4501 aatattgcga ccttaaacag ccgcattgca tcgacagaga aggcgctccc cggtgcaagg
     4561 gctgctgttc aggaagccga taaaaaggta aaagaggcag aggcaaataa agatgatttt
     4621 gtgacttata acccgcctca tgaatatggc tccgggtggc aggatcaggt tcgctatctt
     4681 gataaggata ttcagaatca gaatgagaaa ttaaaagcgg ctcaggcatc tttaaacgca
     4741 atgaatgaat ccttatccag agataaggct gcacttaccg gggcgatgga gagccggaaa
     4801 caaaaggaga aaaaagcgaa ggatgcagaa aataagttaa atgaggaaaa gaacaagcct
     4861 cgcaagggga ctaaagacta cggccatgat tatcatccag tacctaaaac agaagacata
     4921 aagggacttg gagagttaaa gcgaggtgat cctaaaacac ctaaacaagg tggtggtggt
     4981 aagcgggccc gatggtatgg cgataaaaaa cgtaaaattt atgaatggga ttcccagcac
     5041 ggtgagcttg aagggtatcg tgccagtgat ggcgaacacc taggcgcatt tgatcccaaa
     5101 acgggtaagc aggttaaagg gccagatcca aaacggaaca ttaaaaaata tctttgagag
     5161 gtaaatatgg ggcttaaatt acgtttgaca tggtttgata agaaaactga agagtttaaa
     5221 ggggaagagt attctaaaga ctttggtgac gatggttctg tcattgaaag cttgggaatg
     5281 cctttaaagg ataatattaa taatggtgag tttgatttga agaaagaatg ggttccttta
     5341 ctacagccat tttttaaaaa tcaaataaaa cctgatgtgg ataattgtta tatagctttt
     5401 gattatcggg acggtaactg gtaacaaaaa attatcaagg atgatttgaa atactgactt
     5461 ccggtgttca gggatgaatg ccggagtctt taaatacagg gaaagtatat gaaaaaggta
     5521 aagactattt ttctttttat attgattgtt gctggctttc ttctggccgc atgtcaggca
     5581 aattatatcc gggatgttca gggcggaacg gtggcaccat cgtcctcctc tgaactgacg
     5641 gggatcgcgg ttcagtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     5701 taatcttttg ccctgtaaac gaaaaaacca cctggggagg tggtttgatc gaaggttaag
     5761 tcagttgggg aactgcttaa ccgtggtaac tggctttcgc agagcacagc aaccaaatct
     5821 gtccttccag tgtagccgga ctttggcgca cacttcaaga gcaaccgcgt gtttagctaa
     5881 acaaatcctc tgcgaactcc cagttaccaa tggctgctgc cagtggcgtt ttgcgtgctt
     5941 tcccgggttg gactcaagtg aacagttacc ggataaggcg cagcagtcgg gctgaacggg
     6001 gagttcttgc ttacagccca gcttggagcg aacgacctac accgagccga gataccagtg
     6061 tgtgagctat gagaaagcgc cacacttccc gcaagggaga acggcggaca ggtatccggt
     6121 aaacggcagg gtcggaacag gagagcgcaa gagggagcga cctaccggaa acggtgggga
     6181 tctttaagtc ctgtcgggtt tcgcccgtac tgtcagattc atggttgagc ctcacggctc
     6241 ccacagatgc accgaaaaag cgtctgttta tgtgaactct ggcaggaggg cggagcctat
     6301 ggaaaaacgc caccggcgcg gccctgccgt tttgcctcac atgttagtcc cctgcttatc
     6361 cacggaatct gtgggtaact ttgtatgtgt ccgcagcgcc cgccgcagtc tcacgcccgg
     6421 agcgtagcga ccgagtgagc gaggaggcgc aatttccccg gcacctgcaa gacggtcacc
     6481 ggacgcgctg atgtgggata ttgtcccctg attaagtctt tctattccct gacgatcaat
     6541 tatcaggata ggtgaaccat gtcggttttt cgctgaacat tttacgttaa cctatctctg
     6601 ttgctttttt ctccttgcgg agaaatgcaa gtcatccccg ctggggcgtg gctgacttca
     6661 ggggaaaaga ccttgcttac gctggctgga cgccaccgac gcacggtctt ttcccctctt
     6721 tctggctctg tccggctgga cgccggccac gccagaaatt cacccggccc ccgtcaggct
     6781 cacgcctgac cacccacaaa aatccacggt gctggacgca cctgacgaag cagcaaagct
     6841 gctcctgatt ttgcgggtgg tcagccgctc gctatggcga actctgacgg gggccggaac
     6901 accggaaaag accgcactgg ggtgccgcct tttcctctca cacgctgggg cgtgctcgtt
     6961 gttcctccgg ggctgcgcct tttccgcatg gggcggaaag aggctggcct ccgcggtgaa
     7021 agctgaacgc aatcaccacg gacgtgaccg ccgccgccat gccttggggc atgtccgcgg
     7081 cttgtgcagc gggggctgcg catcacgccg gtaaacccgt cgtctgaagg agagagattg
     7141 catgtttaat acggattcgc ttgcctggca gtggcatatc ccggagctgg cccggcagat
     7201 ttacccgtgg gtcagctggc tgtaccggga gtattacagt ctggccggtt tttttctggt
     7261 tatcggcctg tttttcggtc tggctgaact ctgggccggc cgaatgcgtt ttgtggggct
     7321 gagccataag gcgcggcggg agtatgccga tcgctataag cgcgataata accggactgt
     7381 gccattttcg gtgctggcct cgctggattc acccgggcgg atggtcaggg cacggcgaca
     7441 tgtctggaac tacgcgtact ggctgaccgg cgctgccggt ctggcccttc tgggggggct
     7501 ggttgtcgcc gtgtttatcg agagctgggg agaaaccgtc ctgcgcccgg acctctatgc
     7561 gaacgcagat tattacctgc tgcagccggc taacctgttc gggatagcct ggtgtgccgg
     7621 agggcttgtc gccggcgtcc ttctggcgcg gctgctggcg ttcctgctgc ttgatggtca
     7681 ttttgcggcc gctgacgagg cggtgaacgc gaaactgaat caggagagcc ggcgcagcag
     7741 ccagcggacc ggagaaatga cagacgtccg ccacctgcat ttcggggagc cggtaccggt
     7801 taacgcgctg gccgattttt ccacggagca ggcccggaaa cagcaggcgg tgtttctggg
     7861 taaggatgag cagggccagc cggtgctggt ccctcgtgat acctggcgca agaccaatat
     7921 tcagatcctc ggcctgcccg ggagcggcaa gagcgcgatg gccaccaacg cgctcattcg
     7981 ctgcgtacgc gatttcgggg acgctgtggt gtacttcgac cccaaagggg acgcgtgggc
     8041 gccgcacgtc ttccgggcgc actgcccgga cttcaccctg ctggatctgc ggccggggaa
     8101 accggcgcag ctgaatctgt tccgcgatct cgaccagtac gccctgaaga atctgctggt
     8161 tgccgggttt aacctgagtg agaccggcga cgtggcggac cactaccgca tcagcgagca
     8221 gaaagcggcg aagctgattg cggagcagtt cccgcagggg gcaaacattc agcaggtgct
     8281 ggcggcggcg tacgcgttgc cggaggccct gaaaaaggac gtgaaggggc tgatcaccaa
     8341 gctggagaac gtggccgacc tgagcgtcct gcagacggac agcggtatcg atgtggcggg
     8401 gataatcaac ggcggcggct gcctgtacgt catcggctcg atggacgatg aggcggtgat
     8461 ccgggtgcag aagatgctgt ttgcccgctg cgcgcaaatc atcattgcgc gggatgagtt
     8521 ccggaggtgg ccgcacgcga gcatcatgct cgatgagatt aaatatctgc tgtcgaagta
     8581 cgtgctgaac gcgctgggga cgctgcgcag ccgggactgc aacctgctgc tcgcgcacca
     8641 gtcgctgggc gacttcggtc agtgcgggca ggatctgccc gctgattttg tgaaaaccac
     8701 ggtactggat aacacgccga tccgctggtt ctatcgcgcg gccagccagg agtccgcgca
     8761 gtgggcggcg ggccagaccg gggagatccg ggtggacgtg gagcgccgcc gggccagccg
     8821 ggaggcgggg aacgtggagc acatcagcgg cgacagcttc atccagaagg aggcgcggcc
     8881 gctgtttgac gtgaacaccc tgcaacacct gcctgacggg ttcgcggtga tgaccgggct
     8941 gggcgtggcg cggctggcgt tcagcagccc gctgcgggtt gagcgccggg aaatttcgct
     9001 gaaaaccttc ccggtgctgg cgaaaacgga cccgctggcg gagtacctgc ctccggccga
     9061 cggcggcgga ccaaaagcgg atgatgattt tgcggggctt tactgatgac actggagcag
     9121 tacaacgtca gccacgcgaa gcggcaggcg cggaacgcgg agaaaacacg gctgaccctg
     9181 cgctggctgc gggaagagct gtgttccacg gcggagctgg ttgcgcgacg gctggggatt
     9241 gcggcggtac agccggttta ccgcttcctg gacagtctgg tggcgaaagg gctgctggtc
     9301 cgggcgaaat acccggttga cggccgtcag gtcagcgtgt gggggctgac gccgcacggg
     9361 gtggcgttct cgttcgacga ggacgagccg ctgacggacg ttattccgtt tcagccgtca
     9421 agggtgtcgg cggcgcagct gccgcaccgg ctggccgtgc agtccctgcg cctctccatg
     9481 gagggccggg gggccaccgg ctggcggtac ctgcaccgta tggccctgaa agggatgaaa
     9541 gtgccggacg cgctggcgga gctggacggc agaacggtgg catttgaggt agagcgcacc
     9601 gtcaagagcc gcaggcggta tcaggaagtg gtgtccgggt atctgttcaa ccgccgggcg
     9661 aacggcatcg atgaaatctg gtacatctgc cctgaccggc cgacgcttgt ccgggtacag
     9721 cgggcgattc tggcggtgga tgaaatcgtg aatccacaga ccggcgaggc ccgcaaaacg
     9781 gtggagctgg accgggagag gctgttcgcg tgctttaaat ttatgacaac ggagtgaggg
     9841 attatggcgc tgtacatggt atttattgag gacgtggaag acaaagacgg cgagcgctgc
     9901 attatgccaa ccagcgagca tctggaagac atggtggtgc tcgcctggca gaccggggcg
     9961 tatgttttct gttttgtcga ccgggcccag catctggcga tgatgaaatc catcgggatg
    10021 tatgacgagc ggctcaacag cgacgaaaaa gccacgtact ttttcaccgg tgaaaatccg
    10081 caatgggaac gggacaaacc cacaggaatg aagctgtgtg attacctcta tcgaaaggtg
    10141 gagcggttcg ccatcgagaa cggtgtgcag gtggttgatg ccgggaagtg ctcaccggag
    10201 cagattaacg cgatgattga ggtggtcagc gagcggcacg gggaaagcgc ggtgaacgag
    10261 aagctgaaag cggagctgat gcgggaatat aacagccgct attttaacgt gccggtgcct
    10321 gaactgccgg aggacaaacc cgcccgccgg tggtggccgt tcggcaaaaa ataacgccag
    10381 caccggcatg gccggtgctg ttattcttcc ccgagcttat cccgcatcac ctgccagagt
    10441 gtttccgcat cctcgtgaag ccgttcgcac atgtcggccg gggtatccat gtccagctcg
    10501 ttcagctttt ccagcaacag cagcgactga tccttaatga atttagccat attgagggca
    10561 gttttttcct gcttcgtcat gttctccatt cccttcggta agttctcagc taaaccagcc
    10621 ggaccagaag cgttttttct tctgtgtcag ttctgttgtc aatctgtcca cttcggcctg
    10681 gagtttccgg cgctcatcat cgtgctggcg gcgttcttct tcccgggatg ttttatcttc
    10741 cagcatcagg gtgacagcct gccggagatc ggccagttcc ttttgcattg ccagaagctg
    10801 ttcctgtggg aaattttttt gtgtattttc acggtgtgaa atgccgggaa tttgtttctc
    10861 agggatattc acatttccgt atacacggat cagttctgac acatccacga cagggttgtt
    10921 tttcccgtca cgtgacacgg taacctttcc ctgttttaca tgattgtata gcgttcttct
    10981 ggtaatgccc gcggcttgtg ctgcctgtga aaggttgagc aatgttttcg ccactgtata
    11041 cccctgattt tatcgggtgt gaaaggtgtg taatttctca cggtaaatgt atcgctgaat
    11101 tttacccgtt tttccgttca ggatgtggtt ttctgacgcg ctgcgcttgt caaaccggcg
    11161 acggccacgc aatgaattgc gcccccgtca tcggttatta gaggctgtct gcccccggat
    11221 acccggattc tttctgatgc ctgacggtga cgatcacaat ctggtccatt tcccggtcgt
    11281 ggcggtagag catcacatag ccactgtcgc caaacccgat caccagttcc ttatattcca
    11341 gcggcagaaa cggcaccggg cggccaatgt ctggcaatgt tttcagttgc tggatagccc
    11401 ggacgatcac ctcacctcct tttctggctg ccagccgatt tttggttttg agaaagtcct
    11461 gaaggcgttg taaatcgcgc tgtgccagcg cggaaatcgt tacctgtggc atggcggagc
    11521 atcctgctcg ttctcagttc cccaggtact tatccaggct tccgcttctt ccgcagtaag
    11581 gtgcagaccc gtttcctgat agtgctgcca tgcggcctgt ccgtcccgca ggtactggct
    11641 gcgcttctct tcccggtcga tgtattcggt gattgcctcc agcattagcg cgtgcgctga
    11701 gcggtgccgg tcatcggcca gcgttttcag ccggtcctta agttcttcat cgagcctgat
    11761 ggatgttgct gatgacataa aagccccttt gtagtcatat gtattacaca tgactacttt
    11821 aatccgtggc ggcgctattt gccagaaata cggcttttgc tgcccatttt tcagcttcgc
    11881 ttccactaat cagggctgct acaggctcac tcatcatcca ggactattgc agcctgcggg
    11941 ttaaaggacc tgttactggc ggcaaccatc
//
