LOCUS       NZ_CP054760            11970 bp    DNA     circular CON 16-MAY-2024
DEFINITION  Klebsiella pneumoniae strain KP20194c plasmid pKP20194c-p4, complete
            sequence.
ACCESSION   NZ_CP054760
VERSION     NZ_CP054760.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15186441
            Assembly: GCF_013348925.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11970)
  AUTHORS   Hu,L.M., Zhang,X.T., Zeng,X., Chen,Y., Liu,L. and Li,G.Q.
  TITLE     Genomic Characterization of a Carbapenemase-Producing, Extensively
            Drug-Resistant Klebsiella michiganensis Strain from a Renal Abscess
            Patient
  JOURNAL   Microbiol Resour Announc 11 (12), e0082522 (2022)
   PUBMED   36321904
REFERENCE   2  (bases 1 to 11970)
  AUTHORS   Zhang,X., Ouyang,J., He,W., Zeng,T., Liu,B., Jiang,H., Zhang,Y.,
            Zhou,L., Zhou,H., Liu,Z. and Liu,L.
  TITLE     Co-occurrence of Rapid Gene Gain and Loss in an Interhospital
            Outbreak of Carbapenem-Resistant Hypervirulent ST11-K64 Klebsiella
            pneumoniae
  JOURNAL   Front Microbiol 11, 579618 (2020)
   PUBMED   33281772
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 11970)
  AUTHORS   Zhang,X.T. and Liu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2020) Clinical Research institute, The Second
            Affiliated Hospital, University of South China, 35 Libration Ave.,
            Hengyang 421001, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 10-APR-2020
            Assembly Method                   :: SPAdes v. 3.14
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013348925.1-RS_2024_05_16
            Annotation Date                   :: 05/16/2024 02:45:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,903
            CDSs (total)                      :: 5,781
            Genes (coding)                    :: 5,608
            CDSs (with protein)               :: 5,608
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 173
            CDSs (without protein)            :: 173
            Pseudo Genes (ambiguous residues) :: 0 of 173
            Pseudo Genes (frameshifted)       :: 68 of 173
            Pseudo Genes (incomplete)         :: 113 of 173
            Pseudo Genes (internal stop)      :: 23 of 173
            Pseudo Genes (multiple problems)  :: 31 of 173
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP054760.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11970
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP20194c"
                     /serotype="ST11-K64"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP20194c-p4"
                     /geo_loc_name="China: Hunan"
                     /lat_lon="27.72 N 112 E"
                     /collection_date="2019-04-13"
                     /collected_by="Xiaotuan Zhang"
     gene            complement(join(11252..11970,1..13))
                     /gene="mobC"
                     /locus_tag="HUS02_RS29185"
                     /old_locus_tag="HUS02_29190"
     CDS             complement(join(11252..11970,1..13))
                     /gene="mobC"
                     /locus_tag="HUS02_RS29185"
                     /old_locus_tag="HUS02_29190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(13..1977)
                     /locus_tag="HUS02_RS29190"
                     /old_locus_tag="HUS02_29195"
     CDS             complement(13..1977)
                     /locus_tag="HUS02_RS29190"
                     /old_locus_tag="HUS02_29195"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(3461..3610)
                     /locus_tag="HUS02_RS29195"
                     /old_locus_tag="HUS02_29200"
     CDS             complement(3461..3610)
                     /locus_tag="HUS02_RS29195"
                     /old_locus_tag="HUS02_29200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(3695..3952)
                     /locus_tag="HUS02_RS29200"
                     /old_locus_tag="HUS02_29205"
     CDS             complement(3695..3952)
                     /locus_tag="HUS02_RS29200"
                     /old_locus_tag="HUS02_29205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(3962..5647)
                     /locus_tag="HUS02_RS29205"
                     /old_locus_tag="HUS02_29210"
     CDS             complement(3962..5647)
                     /locus_tag="HUS02_RS29205"
                     /old_locus_tag="HUS02_29210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            complement(5752..7239)
                     /gene="ltrA"
                     /locus_tag="HUS02_RS29210"
                     /old_locus_tag="HUS02_29215"
     CDS             complement(5752..7239)
                     /gene="ltrA"
                     /locus_tag="HUS02_RS29210"
                     /old_locus_tag="HUS02_29215"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            7884..8129
                     /locus_tag="HUS02_RS29215"
                     /old_locus_tag="HUS02_29220"
     CDS             7884..8129
                     /locus_tag="HUS02_RS29215"
                     /old_locus_tag="HUS02_29220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            8403..8774
                     /gene="tolA"
                     /locus_tag="HUS02_RS29220"
                     /old_locus_tag="HUS02_29225"
     CDS             8403..8774
                     /gene="tolA"
                     /locus_tag="HUS02_RS29220"
                     /old_locus_tag="HUS02_29225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            8771..9136
                     /locus_tag="HUS02_RS29620"
     CDS             8771..9136
                     /locus_tag="HUS02_RS29620"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            9311..9589
                     /locus_tag="HUS02_RS29230"
                     /old_locus_tag="HUS02_29235"
     CDS             9311..9589
                     /locus_tag="HUS02_RS29230"
                     /old_locus_tag="HUS02_29235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            9577..9891
                     /locus_tag="HUS02_RS29235"
                     /old_locus_tag="HUS02_29240"
     CDS             9577..9891
                     /locus_tag="HUS02_RS29235"
                     /old_locus_tag="HUS02_29240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            10055..10483
                     /locus_tag="HUS02_RS29240"
                     /old_locus_tag="HUS02_29245"
     CDS             10055..10483
                     /locus_tag="HUS02_RS29240"
                     /old_locus_tag="HUS02_29245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            10509..10688
                     /locus_tag="HUS02_RS29245"
                     /old_locus_tag="HUS02_29250"
     CDS             10509..10688
                     /locus_tag="HUS02_RS29245"
                     /old_locus_tag="HUS02_29250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(10715..11245)
                     /locus_tag="HUS02_RS29250"
                     /old_locus_tag="HUS02_29255"
     CDS             complement(10715..11245)
                     /locus_tag="HUS02_RS29250"
                     /old_locus_tag="HUS02_29255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     BGC             1..10647
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          2780..3358
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(11256..11968)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.118"
                     /coverage="98.0"
                     /evalue="1.21e-138"
ORIGIN
        1 gctccagtgt catcagtaaa gccccgcaaa atcatcatcc gcttttggtc cgccgccgtc
       61 ggccggaggc aggtactccg ccagcgggtc cgttttcgcc agcaccggga aggttttcag
      121 cgaaatttcc cggcgctcaa cccgcagcgg gctgctgaac gccagccgcg ccacgcccag
      181 cccggtcatc accgcgaacc cgtcaggcag gtgttgcagg gtgttcacgt caaacagcgg
      241 ccgcgcctcc ttctggatga agctgtcgcc gctgatgtgc tccacgttcc ccgcctcccg
      301 gctggcccgg cggcgctcca cgtccacccg gatctccccg gtctggcccg ccgcccactg
      361 cgcggactcc tggctggccg cgcgatagaa ccagcggatc ggcgtgttat ccagtaccgt
      421 ggttttcaca aaatcagcgg gcagatcctg cccgcactga ccgaagtcgc ccagcgactg
      481 gtgcgcgagc agcaggttgc agtcccggct gcgcagcgtc cccagcgcgt tcagcacgta
      541 cttcgacagc agatatttaa tctcatcgag catgatgctc gcgtgcggcc acctccggaa
      601 ctcatcccgc gcaatgatga tttgcgcgca gcgggcaaac agcatcttct gcacccggat
      661 caccgcctca tcgtccatcg agccgatgac gtacaggcag ccgccgccgt tgattatccc
      721 cgccacatcg ataccgctgt ccgtctgcag gacgctcagg tcggccacgt tctccagctt
      781 ggtgatcagc cccttcacgt cctttttcag ggcctccggc aacgcgtacg ccgccgccag
      841 cacctgctga atgtttgccc cctgcgggaa ctgctccgca atcagcttcg ccgctttctg
      901 ctcgctgatg cggtagtggt ccgccacgtc gccggtctca ctcaggttaa acccggcaac
      961 cagcagattc ttcagggcgt actggtcgag atcgcggaac agattcagct gcgccggttt
     1021 ccccggccgc agatccagca gggtgaagtc cgggcagtgc gcccggaaga cgtgcggcgc
     1081 ccacgcgtcc cctttggggt cgaagtacac cacagcgtcc ccgaaatcgc gtacgcagcg
     1141 aatgagcgcg ttggtggcca tcgcgctctt gccgctcccg ggcaggccga ggatctgaat
     1201 attggtcttg cgccaggtat cacgagggac cagcaccggc tggccctgct catccttacc
     1261 cagaaacacc gcctgctgtt tccgggcctg ctccgtggaa aaatcggcca gcgcgttaac
     1321 cggtaccggc tccccgaaat gcaggtggcg gacgtctgtc atttctccgg tccgctggct
     1381 gctgcgccgg ctctcctgat tcagtttcgc gttcaccgcc tcgtcagcgg ccgcaaaatg
     1441 accatcaagc agcaggaacg ccagcagccg cgccagaagg acgccggcga caagccctcc
     1501 ggcacaccag gctatcccga acaggttagc cggctgcagc aggtaataat ctgcgttcgc
     1561 atagaggtcc gggcgcagga cggtttctcc ccagctctcg ataaacacgg cgacaaccag
     1621 cccccccaga agggccagac cggcagcgcc ggtcagccag tacgcgtagt tccagacatg
     1681 tcgccgtgcc ctgaccatcc gcccgggtga atccagcgag gccagcaccg aaaatggcac
     1741 agtccggtta ttatcgcgct tatagcgatc ggcatactcc cgccgcgcct tatggctcag
     1801 ccccacaaaa cgcattcggc cggcccagag ttcagccaga ccgaaaaaca ggccgataac
     1861 cagaaaaaaa ccggccagac tgtaatactc ccggtacagc cagctgaccc acgggtaaat
     1921 ctgccgggcc agctccggga tatgccactg ccaggcaagc gaatccgtat taaacatgca
     1981 atctctctcc ttcagacgac gggtttaccg gcgtgatgcg cagcccccgc tgcacaagcc
     2041 gcggacatgc cccaaggcat ggcggcggcg gtcacgtccg tggtgattgc gttcagcttt
     2101 caccgcggag gccagcctct ttccgcccca tgcggaaaag gcgcagcccc ggaggaacaa
     2161 cgagcacgcc ccagcgtgtg agaggaaaag gcggcacccc agtgcggtct tttccggtgt
     2221 tccggccccc gtcagagttc gccatagcga gcggctgacc acccgcaaaa tcaggagcag
     2281 ctttgctgct tcgtcaggtg cgtccagcac cgtggatttt tgtgggtggt caggcgtgag
     2341 cctgacgggg gccgggtgaa tttctggcgt ggccggcgtc cagccggaca gagccagaaa
     2401 gaggggaaaa gaccgtgcgt cggtggcgtc cagccagcgt aagcaaggtc ttttcccctg
     2461 aagtcagcca cgccccagcg gggatgactt gcatttctcc gcaaggagaa aaaagcaaca
     2521 gagataggtt aacgtaaaat gttcagcgaa aaaccgacat ggttcaccta tcctgataat
     2581 tgatcgtcag ggaatagaaa gacttaatca ggggacaata tcccacatca gcgcgtccgg
     2641 tgaccgtctt gcaggtgccg gggaaattgc gcctcctcgc tcactcggtc gctacgctcc
     2701 gggcgtgaga ctgcggcggg cgctgcggac acatacaaag ttacccacag attccgtgga
     2761 taagcagggg actaacatgt gaggcaaaac ggcagggccg cgccggtggc gtttttccat
     2821 aggctccgcc ctcctgccag agttcacata aacagacgct ttttcggtgc atctgtggga
     2881 gccgtgaggc tcaaccatga atctgacagt acgggcgaaa cccgacagga cttaaagatc
     2941 cccaccgttt ccggtaggtc gctccctctt gcgctctcct gttccgaccc tgccgtttac
     3001 cggatacctg tccgccgttc tcccttgcgg gaagtgtggc gctttctcat agctcacaca
     3061 ctggtatctc ggctcggtgt aggtcgttcg ctccaagctg ggctgtaagc aagaactccc
     3121 cgttcagccc gactgctgcg ccttatccgg taactgttca cttgagtcca acccgggaaa
     3181 gcacgcaaaa cgccactggc agcagccatt ggtaactggg agttcgcaga ggatttgttt
     3241 agctaaacac gcggttgctc ttgaagtgtg cgccaaagtc cggctacact ggaaggacag
     3301 atttggttgc tgtgctctgc gaaagccagt taccacggtt aagcagttcc ccaactgact
     3361 taaccttcga tcaaaccacc tccccaggtg gttttttcgt ttacagggca aaagattacg
     3421 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctactgaacc gcgatccccg
     3481 tcagttcaga ggaggacgat ggtgccaccg ttccgccctg aacatcccgg atataatttg
     3541 cctgacatgc ggccagaaga aagccagcaa caatcaatat aaaaagaaaa atagtcttta
     3601 cctttttcat atactttccc tgtatttaaa gactccggca ttcatccctg aacaccggaa
     3661 gtcagtattt caaatcatcc ttgataattt tttgttacca gttaccgtcc cgataatcaa
     3721 aagctatata acaattatcc acatcaggtt ttatttgatt tttaaaaaat ggctgtagta
     3781 aaggaaccca ttctttcttc aaatcaaact caccattatt aatattatcc tttaaaggca
     3841 ttcccaagct ttcaatgaca gaaccatcgt caccaaagtc tttagaatac tcttcccctt
     3901 taaactcttc agttttctta tcaaaccatg tcaaacgtaa tttaagcccc atatttacct
     3961 ctcaaagata ttttttaatg ttccgttttg gatctggccc tttaacctgc ttacccgttt
     4021 tgggatcaaa tgcgcctagg tgttcgccat cactggcacg atacccttca agctcaccgt
     4081 gctgggaatc ccattcataa attttacgtt ttttatcgcc ataccatcgg gcccgcttac
     4141 caccaccacc ttgtttaggt gttttaggat cacctcgctt taactctcca agtcccttta
     4201 tgtcttctgt tttaggtact ggatgataat catggccgta gtctttagtc cccttgcgag
     4261 gcttgttctt ttcctcattt aacttatttt ctgcatcctt cgcttttttc tccttttgtt
     4321 tccggctctc catcgccccg gtaagtgcag ccttatctct ggataaggat tcattcattg
     4381 cgtttaaaga tgcctgagcc gcttttaatt tctcattctg attctgaata tccttatcaa
     4441 gatagcgaac ctgatcctgc cacccggagc catattcatg aggcgggtta taagtcacaa
     4501 aatcatcttt atttgcctct gcctctttta cctttttatc ggcttcctga acagcagccc
     4561 ttgcaccggg gagcgccttc tctgtcgatg caatgcggct gtttaaggtc gcaatatttt
     4621 tatcttcggc atccagctca gctttcgctt tatcatagtc tcttttcagc ccctcttccg
     4681 gatgagcggc gtcccatgcc tgctggcgac gcttttcttc ctcctgacgc tgttttacct
     4741 gtgccggggt aagaacatct gtcaccgaca cataaaccgg cttctgtccg gtctctttcg
     4801 ggaaacgaat aacggcctca cgggagttac caccggctgt aaaaccagcc ggacgtgaat
     4861 cacctttttc agcaacaatg ccttttggcg gggctttcgc tgtcggaaca cctttcggta
     4921 cgctcacctg cagagacggg agacccggaa tcgacacact gaataccccc ggacgttttg
     4981 tcggtttcgc atccaccaca gggacactca ttggccgacc tgcgacaacc gcaatatgct
     5041 gccgctcatc cttcaccaca tccacaaccc gttgtctgac acgaaccgtc gcctggtcca
     5101 gtggtaaagt acttgccgga gtctccgtca ccgcatcggc cggtaatgac gtcataattt
     5161 ttgacatcat tttcggatca tcttttgcaa tctcagaagg cagcacaccg tagatcgcaa
     5221 tcccccacag accaaactta aacggcccct tcagggcagc cagcacatca gcaacggcgg
     5281 ccgacaacgc atcgccggaa actgataaag ccagtccttc agcccccgga gtagccaggg
     5341 caggcagacc gaaggccatc gcagtggccg aagactgtcc gccaccagag ctaccgctat
     5401 gattgccgct gttaccacca ttaccaccgg gtttactccc gccgttacca aactcccccg
     5461 gattatagtt atgaatatct ccattcggcg tatgcgtcgt accccagccc ggcccgctgt
     5521 ttggatccgt accaccacct gatgaagggc cacctttacc agacgtaccg ttcacattcc
     5581 cactggcctg accaccatta tgtcccagac ctgaattacc cggacctcga ccgtctccac
     5641 cactcatacc gttttcctct ttgacattta aaacaataaa ttaaagccaa catgtcgcgg
     5701 tagggatacc cgttaccgga taccccccgc acagatcccg gcgtgcgcga tttacgcacc
     5761 gggctcctgc ctcgggtgtc tggcggtgaa ccgctccaca ggccatggat gaagaacccg
     5821 aacccttggt agccatgcgg ctgccagttt gtttgctttc gtccaggtcg tatcatcctt
     5881 ctggctcctg cgcctgagcg cccggcgcca gaggtttgtt acgtgtgtcc tgaacttctg
     5941 catggtgggg aagttgcccg gtaccgagtg atagttcagg tatccctgaa ccactctcct
     6001 gagccatttt ccctgttcgg ggattgagta atgccagcgc cttcgcagac cgtctttgat
     6061 ggctttcaga gttgccgtca tccgatcccg gcgggtcttt cgtatcagca tgaacctgcc
     6121 gttgcgatct ttcccgctga tgtgcgtgaa cccgaggaag ttgaacgttt ctggtttgcc
     6181 ttttcccctg atggcacggt tttcggcagc gaagcggccg aactccatca gacgggtttt
     6241 ctccgggtga accgtgagtc cgaactccct cagtctgcgc tgcatggcta tacggaagcg
     6301 ccgggcatcg tatcgtttgt cgaacccgat gacgatgtca tcggcgtatc tgaccattac
     6361 cacattgcct gtggcatagc gacgtcgcca ctgatgcgcc cacagatcga agacgtagtg
     6421 gaggtatatg tttgccagca gcggtgagat gaccgcaccc tgtggggtgc cttcctccgt
     6481 tgctcgccat tgaccctcct ccgacgtccc ggctgtgagc cacttacgta tgagcctgat
     6541 taccctccgg tcgccgatcc gatgctctgt gaacctgatc agccattcgt ggctcaccct
     6601 gtcgaagaac tgactgatgt cggcatccag tacccagttt acgttagtgc gtaccagccc
     6661 tgtggccagt gcgtccagtg catcgtgctg gcttcgcccg ggtctgaacc cgtatgagaa
     6721 ccccataaag tcgttttcat agactgcgtt caggattttc accagcgcat actggacgat
     6781 cttgtcctcc agcgaggcga tgccgagcgg gcgttgtttt ccatccgctt ttgggatgta
     6841 gtgacgcctg ccgggctgcg ccctgtagct gccctgatgt agcctccggt gcagatctgt
     6901 tatgttgttc ttcatgtttc cggcgtagtc catccacctg atgccatcca ctccggcggc
     6961 cgctttcctg ctcagggaga ggaatgcggc ttccagtgct tcgactgtca gcaggtggaa
     7021 caatgctgta aaccgttctt tcttccgctg cttcgcagct tcccgcacgc gtgacagcct
     7081 ctgtgacatg ctttcccggc tctgtgtccg gcgcatgtgt ggctgttccg cgttcccctt
     7141 ggccccgctc cttcgctcca ctgactccgc tcctttcggg ttgttcgcct gcttcgccgc
     7201 tactatgagc gagtccgact tctcctctcc gtacatcacc ggctatgact cctcgtcttc
     7261 ccggtgcggg ccatctccga cactggcaga tggtcagagg ggagatctcc cggttcccgc
     7321 gtagagatcg tattgacatg ccagggtctc agaccccgcc gggtccatgt ggcactcgca
     7381 gtgtcgcacc ctatgatgtt gccttccgtt aacagtacaa cgtcggcacc cggtaattta
     7441 atatacattt cgtggctcaa tggctggcct gtcaacaccc ctgtcaacgc ttcgccccat
     7501 acctcgcggt atgcaacgca tgactcgggg accttgtgga ttgctggtcc ttcaatggtc
     7561 ggggactttc accccttgat ctctaaccgg tctcccggcg cacactgtac atatacacag
     7621 tataattatt accttactcc tcccgttttc aggtgtcaat atatgagatt agttctcatc
     7681 ttcaggcaca aaaaagcgcc cggagccacc tgtaagcaga tcgcgtcttt tcccgaaaat
     7741 ttctcatggc aacaaatttt attatttaag acattcatca cacttacatt tcagatggta
     7801 tagacacatc tgaaagctaa ctggcatgag gagaggacct gcggttaaac tgttcgggaa
     7861 acctgagacg cccggaggcc actatgacgc acctgaaact gactcccgct gaagaaaagc
     7921 gcctggcgga cgcctttgtc gctcaggcac tggccatggg cctgaaaggg aacaaaacgc
     7981 tgccaaaggc ggtaaaacaa caggtgcggg aaagggtgct tcgtgccatt caggacgaac
     8041 gtgaagccag gcgggcgcag gaaatgcgtc gggagaaata cgaggctacg gaaaccacct
     8101 tcacctggca gccacagcgc cgccggtaag gggtatttac cataacgtcg cattgcccgc
     8161 accgctcaga cggtctgagg gcgtttccgg cggctacggg ataaaacagg caacaaacca
     8221 cccggaaaac cgccggaaac gcgcccggta aattgtaacc cgctgcatgc ctatgcacgc
     8281 aactgcatgg cgggccgttt ggtgtaaaaa cgatacgata agccgtgttt atgtaaatga
     8341 acgtggccgt aaagagtgac aaatatacat gtaacatttt ttactgacag gtcatcaacc
     8401 cgatgcgttt aatacccttt atcctgctgg cattgtgcag ccccttactc ctgacggcct
     8461 gtaagcattc caccacccag ccgccgaaaa tttatactct ggataagtca tctaaaatca
     8521 gtagctacgc tgcggccatc aagaccgctg ttgaaatgca gatgtttgaa ccagaggcct
     8581 atgcgggaaa aatgtgcacc gtgcggatat ccatacaacg tgatggcact ctgaacagcg
     8641 ccattgctga aggaggagat ccgaaactct gtaaagcagc aatatcagcc gttacccacg
     8701 ccaaaattcc gccggcaccg gatgaaaata cctggcaaat attcaaaaac gcccctctgg
     8761 atttcagacc atgaaagctt ttactctgac agtgctggcg gccatgttcc tcactccggt
     8821 acatgcgata ccactaaaac ccgtaaccac actgcagcca acctacccgg tacaggccgc
     8881 agcaaaacgc atcgaaggtc aggtgaccgc cacgtttgat attctggacg atggacgagt
     8941 ggacaacgtg tcgcttaccg gctgtcacct tttttttcgg gaaacgctga cggcaatgag
     9001 gcactggcta tatcccgcag ggcacccggc aatgcgacag gttataaatt ttgaattcag
     9061 tctgaaaaat gacggcttac aggatccgcg accagtcacc acccaaaagc tcaatgatga
     9121 tggttgccgc cagtaacagg tcctttaacc cgcaggctgc aatagtcctg gatgatgagt
     9181 gagcctgtag cagccctgat tagtggaagc gaagctgaaa aatgggcagc aaaagccgta
     9241 tttctggcaa atagcgccgc cacggattaa agtagtcatg tgtaatacat atgactacaa
     9301 aggggctttt atgtcatcag caacatccat caggctcgat gaagaactta aggaccggct
     9361 gaaaacgctg gccgatgacc ggcaccgctc agcgcacgcg ctaatgctgg aggcaatcac
     9421 cgaatacatc gaccgggaag agaagcgcag ccagtacctg cgggacggac aggccgcatg
     9481 gcagcactat caggaaacgg gtctgcacct tactgcggaa gaagcggaag cctggataag
     9541 tacctgggga actgagaacg agcaggatgc tccgccatgc cacaggtaac gatttccgcg
     9601 ctggcacagc gcgatttaca acgccttcag gactttctca aaaccaaaaa tcggctggca
     9661 gccagaaaag gaggtgaggt gatcgtccgg gctatccagc aactgaaaac attgccagac
     9721 attggccgcc cggtgccgtt tctgccgctg gaatataagg aactggtgat cgggtttggc
     9781 gacagtggct atgtgatgct ctaccgccac gaccgggaaa tggaccagat tgtgatcgtc
     9841 accgtcaggc atcagaaaga atccgggtat ccgggggcag acagcctcta ataaccgatg
     9901 acgggggcgc aattcattgc gtggccgtcg ccggtttgac aagcgcagcg cgtcagaaaa
     9961 ccacatcctg aacggaaaaa cgggtaaaat tcagcgatac atttaccgtg agaaattaca
    10021 cacctttcac acccgataaa atcaggggta tacagtggcg aaaacattgc tcaacctttc
    10081 acaggcagca caagccgcgg gcattaccag aagaacgcta tacaatcatg taaaacaggg
    10141 aaaggttacc gtgtcacgtg acgggaaaaa caaccctgtc gtggatgtgt cagaactgat
    10201 ccgtgtatac ggaaatgtga atatccctga gaaacaaatt cccggcattt cacaccgtga
    10261 aaatacacaa aaaaatttcc cacaggaaca gcttctggca atgcaaaagg aactggccga
    10321 tctccggcag gctgtcaccc tgatgctgga agataaaaca tcccgggaag aagaacgccg
    10381 ccagcacgat gatgagcgcc ggaaactcca ggccgaagtg gacagattga caacagaact
    10441 gacacagaag aaaaaacgct tctggtccgg ctggtttagc tgagaactta ccgaagggaa
    10501 tggagaacat gacgaagcag gaaaaaactg ccctcaatat ggctaaattc attaaggatc
    10561 agtcgctgct gttgctggaa aagctgaacg agctggacat ggataccccg gccgacatgt
    10621 gcgaacggct tcacgaggat gcggaaacac tctggcaggt gatgcgggat aagctcgggg
    10681 aagaataaca gcaccggcca tgccggtgct ggcgttattt tttgccgaac ggccaccacc
    10741 ggcgggcggg tttgtcctcc ggcagttcag gcaccggcac gttaaaatag cggctgttat
    10801 attcccgcat cagctccgct ttcagcttct cgttcaccgc gctttccccg tgccgctcgc
    10861 tgaccacctc aatcatcgcg ttaatctgct ccggtgagca cttcccggca tcaaccacct
    10921 gcacaccgtt ctcgatggcg aaccgctcca cctttcgata gaggtaatca cacagcttca
    10981 ttcctgtggg tttgtcccgt tcccattgcg gattttcacc ggtgaaaaag tacgtggctt
    11041 tttcgtcgct gttgagccgc tcgtcataca tcccgatgga tttcatcatc gccagatgct
    11101 gggcccggtc gacaaaacag aaaacatacg ccccggtctg ccaggcgagc accaccatgt
    11161 cttccagatg ctcgctggtt ggcataatgc agcgctcgcc gtctttgtct tccacgtcct
    11221 caataaatac catgtacagc gccataatcc ctcactccgt tgtcataaat ttaaagcacg
    11281 cgaacagcct ctcccggtcc agctccaccg ttttgcgggc ctcgccggtc tgtggattca
    11341 cgatttcatc caccgccaga atcgcccgct gtacccggac aagcgtcggc cggtcagggc
    11401 agatgtacca gatttcatcg atgccgttcg cccggcggtt gaacagatac ccggacacca
    11461 cttcctgata ccgcctgcgg ctcttgacgg tgcgctctac ctcaaatgcc accgttctgc
    11521 cgtccagctc cgccagcgcg tccggcactt tcatcccttt cagggccata cggtgcaggt
    11581 accgccagcc ggtggccccc cggccctcca tggagaggcg cagggactgc acggccagcc
    11641 ggtgcggcag ctgcgccgcc gacacccttg acggctgaaa cggaataacg tccgtcagcg
    11701 gctcgtcctc gtcgaacgag aacgccaccc cgtgcggcgt cagcccccac acgctgacct
    11761 gacggccgtc aaccgggtat ttcgcccgga ccagcagccc tttcgccacc agactgtcca
    11821 ggaagcggta aaccggctgt accgccgcaa tccccagccg tcgcgcaacc agctccgccg
    11881 tggaacacag ctcttcccgc agccagcgca gggtcagccg tgttttctcc gcgttccgcg
    11941 cctgccgctt cgcgtggctg acgttgtact
//
