LOCUS       NZ_CP054755             5596 bp    DNA     circular CON 16-MAY-2024
DEFINITION  Klebsiella pneumoniae strain KP20194c3 plasmid pKP20194c3-p5,
            complete sequence.
ACCESSION   NZ_CP054755
VERSION     NZ_CP054755.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15186442
            Assembly: GCF_013349025.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Hu,L.M., Zhang,X.T., Zeng,X., Chen,Y., Liu,L. and Li,G.Q.
  TITLE     Genomic Characterization of a Carbapenemase-Producing, Extensively
            Drug-Resistant Klebsiella michiganensis Strain from a Renal Abscess
            Patient
  JOURNAL   Microbiol Resour Announc 11 (12), e0082522 (2022)
   PUBMED   36321904
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Zhang,X., Ouyang,J., He,W., Zeng,T., Liu,B., Jiang,H., Zhang,Y.,
            Zhou,L., Zhou,H., Liu,Z. and Liu,L.
  TITLE     Co-occurrence of Rapid Gene Gain and Loss in an Interhospital
            Outbreak of Carbapenem-Resistant Hypervirulent ST11-K64 Klebsiella
            pneumoniae
  JOURNAL   Front Microbiol 11, 579618 (2020)
   PUBMED   33281772
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5596)
  AUTHORS   Zhang,X.T. and Liu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2020) Clinical Research institute, The Second
            Affiliated Hospital, University of South China, 35 Libration Ave.,
            Hengyang 421001, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 23-JAN-2020
            Assembly Method                   :: SPAdes v. 3.14
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013349025.1-RS_2024_05_16
            Annotation Date                   :: 05/16/2024 02:47:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,920
            CDSs (total)                      :: 5,798
            Genes (coding)                    :: 5,619
            CDSs (with protein)               :: 5,619
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 68 of 179
            Pseudo Genes (incomplete)         :: 119 of 179
            Pseudo Genes (internal stop)      :: 24 of 179
            Pseudo Genes (multiple problems)  :: 32 of 179
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP054755.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP20194c3"
                     /serotype="ST11-K64"
                     /isolation_source="bed sheets"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP20194c3-p5"
                     /geo_loc_name="China: Hunan"
                     /lat_lon="27.72 N 112 E"
                     /collection_date="2019-04-15"
                     /collected_by="Xiaotuan Zhang"
     gene            complement(join(5249..5596,1..198))
                     /locus_tag="HUS03_RS29330"
                     /old_locus_tag="HUS03_29335"
     CDS             complement(join(5249..5596,1..198))
                     /locus_tag="HUS03_RS29330"
                     /old_locus_tag="HUS03_29335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(224..550)
                     /locus_tag="HUS03_RS29335"
                     /old_locus_tag="HUS03_29340"
     CDS             complement(224..550)
                     /locus_tag="HUS03_RS29335"
                     /old_locus_tag="HUS03_29340"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            complement(908..1066)
                     /locus_tag="HUS03_RS29925"
     CDS             complement(908..1066)
                     /locus_tag="HUS03_RS29925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            1706..1882
                     /locus_tag="HUS03_RS29340"
                     /old_locus_tag="HUS03_29345"
     CDS             1706..1882
                     /locus_tag="HUS03_RS29340"
                     /old_locus_tag="HUS03_29345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            1872..2216
                     /locus_tag="HUS03_RS29345"
                     /old_locus_tag="HUS03_29350"
     CDS             1872..2216
                     /locus_tag="HUS03_RS29345"
                     /old_locus_tag="HUS03_29350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2425..>2859
                     /locus_tag="HUS03_RS29785"
                     /pseudo=""
     CDS             2425..>2859
                     /locus_tag="HUS03_RS29785"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2894..3403
                     /locus_tag="HUS03_RS29355"
                     /old_locus_tag="HUS03_29360"
     CDS             2894..3403
                     /locus_tag="HUS03_RS29355"
                     /old_locus_tag="HUS03_29360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            3410..3622
                     /locus_tag="HUS03_RS29360"
                     /old_locus_tag="HUS03_29365"
     CDS             3410..3622
                     /locus_tag="HUS03_RS29360"
                     /old_locus_tag="HUS03_29365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            4289..4513
                     /locus_tag="HUS03_RS29365"
                     /old_locus_tag="HUS03_29370"
     CDS             4289..4513
                     /locus_tag="HUS03_RS29365"
                     /old_locus_tag="HUS03_29370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            4497..4763
                     /locus_tag="HUS03_RS29370"
                     /old_locus_tag="HUS03_29375"
     CDS             4497..4763
                     /locus_tag="HUS03_RS29370"
                     /old_locus_tag="HUS03_29375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(4833..5183)
                     /locus_tag="HUS03_RS29375"
                     /old_locus_tag="HUS03_29380"
     CDS             complement(4833..5183)
                     /locus_tag="HUS03_RS29375"
                     /old_locus_tag="HUS03_29380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     Typing          1541..1620
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(958..1355)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          2207..3738
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tttcagagac tctacaccgt gtatgttgta tgagataaaa tgtaataaat taagatgcaa
       61 tgcaattaat ccatttttgt ttacatctga gtatttggat aaaaaacgta atgaagtgtt
      121 aactctccct tgaagcctaa caattgggag tgctgccttg tatatttcct tctccgttaa
      181 ataccgttta aaaaacataa ttctccgcct ccgtatagcg tgcctatttt atttcgtcgt
      241 aaaacttagc tctgaaatat tcctttagaa ccatcatgtc attaataatt gaatctatct
      301 catccatttt ttcttggtat tcttttaatg gtgaagggag cggtaactca atatttgaaa
      361 agtgattggc gagataggga tcaaggtttc tcatccattc aggcttttcg ccaatgcgtt
      421 cttcaactga aagtgaacct gataatctct ggttaataag atcacttaga ctcattttgg
      481 ttttattcga aagccgttgt aaggattgct ttaatcctat atctagcatg atatttgttc
      541 tttctctcat taccccccct tgctattgcg ttttttgttg cgcacaaagc taccattgcg
      601 cattaagaaa cgcaataaaa aaggagggag ggagtggttt gtacatgatg tgcagtgcgc
      661 tgagggcatc agtggcgggt gtttttccgt gggatggatg gagctccggg gataacagaa
      721 ctgacgatca tttaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctcttgcac
      781 tgtaaacgaa aaaaccgcct agggaggcgg tttgatcgaa ggttaagtca gttggggaac
      841 tgcttaaccg ggtaactggg tatacgagac cgcagatacc aaaactgttc tttcagtgta
      901 gccgcagtta ggcacccact tcaagacctc tcaatatctc tctcgtatat atccagttac
      961 caatggctgc tgccagtggc gtttttgcgt gtccgtccgg gttggactca agatgatagt
     1021 taccggaaag ggcgcagcag tcgggctgaa cggggggttc gtgcatacag cccagcttgg
     1081 agcgaacgac ctaaaccgag ccgagatacc agcagcgtga gctatgagaa agcgccacgc
     1141 ttcccgaaag ggaaaaaggc ggaacaggta tccggtaaac ggcagggtcg gaacaggaga
     1201 gcgcacgagg gagccgccgg agagaaatca ccggcatctt taagtcctgt cgggtttcgt
     1261 cctcctctga cttgagcgtc catttctgtg atgctcgtca ggggaggcgg agcctatgga
     1321 aaaacggcaa cgccgcatcc ttttctccgg ccctttctga tttgagcgcc tttgctttat
     1381 cccgttatcc gtgagtcggt cacgcccgcc gcagccgaac ggcaggagcg cagctaccga
     1441 gtgagcgagg aggcgtcatt tcatctgtcc gtgcatggta ttgcggacgc tcctgagcca
     1501 catatcacac ctgccacatg aggcactttc atgaatcgtg ctctcatgcc aacatagtaa
     1561 gccagtatac acagcgtaaa cgctgtgact ggttcagggc tgcgccccga aacccgctaa
     1621 caccactgac gcgctggcgc ttgtccaacg ccggagcgac ccggaaaact tttccgtgcc
     1681 gtccggcgtt tgcgaaggag ggataatgca gaagccgaag aagaagccgc tcgtcgtaaa
     1741 aaaaagatcc tggcatccgg cagtgatggc aaatctgatg cgtcaggcac aggaggaaga
     1801 ttaccgccgg gagcagatgg cagagcttga gcgttcagcc cggtcacatt ttgcggatat
     1861 cggaggagag gatggcggtt aagcgtaccc gttttttagg tatccgggtg accgatgggg
     1921 agtaccagca gctgctggag cgctgcaatg gaaggcaact ggcggtgtgg atgcgggaaa
     1981 cctgtctcga tacgcgcccg gcgcggtcgt tgcggctgcc gtccatcgat ccggttttgc
     2041 tgcgccagct tgccgggatg gggaacaacc tcaaccagat cgcccggaaa attaacggcg
     2101 gccagtggtc aggcgctgac cgggttcagg tggtggccgc gctgatggcc attgatgccg
     2161 aactcgagcg gctgcggcat gccgtgctgg aaaagggggc agacgatgat cgttaagttt
     2221 catccgcgag ggcgaggcgg cggtgccggg ccggtggatt atctgctggg gaaagaccgc
     2281 cagcgcgacg gggccatcgt cctgcaggga aagccggagg aggtccggga gcttatcgac
     2341 gcctcgccct acgccaaaaa gtacacctcc ggcgtcctgt cttttgctga acaggattta
     2401 ccgcccggcc agcgtgaaaa gctgatggcg agcttcgagc gggttctgat gcccggactc
     2461 gataaagacc agtacagcgt gctgtgggtt gaacaccggg acaaggggcg gctggaactg
     2521 aattttctgg taccgaacac ggaactgctg accggcaggc gtctccagcc gtactacgac
     2581 cgggcagacc gtccgcgcat cgatgcgtgg cagaccgtgg tgaacgggcg actggggctg
     2641 cacgacccga acgcaccgga gaaccggcgc gcgctggtga cgccatcttc gttacccaaa
     2701 acgaagcagg aagccgcaga ggctattacg cggggcttac tggccctcgc ctcgtcaggg
     2761 gagcttaaaa cgcgtcagga cgtcactgag gcgctggaaa gcgcaggttt tgaggtcgtg
     2821 cgcaccacaa agagcagcat cagcattgcc gacccggacg gagggcgaaa catccgactt
     2881 aagggagcca tctatgaaca gtcttttaac gctggcgaag gacttagagc agaaatcgaa
     2941 agcgcagcag cagagtaccg gcgaaatgct gaaagccgca ttcagcgagc acgagaagtc
     3001 tgtcagagcg gaactgagcg aaagcgggaa gagaatcagc gccgccatcc gcgcccacga
     3061 gcagggtatg agcgcggcca tgcagtcgaa ccgcctgagc gtgatgcgta tggtcgggcg
     3121 gacatggctg accatcacga tggtgtcagg actgctgttc gccagcctga gcggggtgtt
     3181 gtggtatcag gggagcttga tagcgtcaaa tctggccgaa atcgaccggc agaacgcagc
     3241 gctgtcgaag ctgaacgcga agacctgggg cgtgacgtac ctggaagaca gcaacggacg
     3301 ttttctggtg ctgccgaagg ggacggccgt agacaggacg cagagctgga cggtcgggaa
     3361 cgggcggtcg aagcagaacg cgctgagact ggtgaaggag taagtgccga tgaccgagct
     3421 ggaaaaacag ttgctgagcg cattagagca gctacagcag gactactcga aaaggctgga
     3481 cgagtgggag agcgccttcg gggaatggcg gaaaatgtca gggcttatgc aacgggagaa
     3541 cgcggcgctg agcgagcgcg tgacgcgctt gagtcagcag gtggaacgct tgagcgggca
     3601 actgcagcgt ttgagccggt agtgcagcgt catgagatgg ccgtggccgc agaacgggcg
     3661 cacgagcaac ggcagcacga aaaggaactg acggaagcgc gttcccggca gcgaagctat
     3721 gacggtccgt cgctgggctg aaatgcagga aaacagggtt ctcatcgggc atttacgctg
     3781 aaaccggatg tcattcacct ggctgcatgg ctatgcagcc aggtaaaatt tatctgtcgc
     3841 gaacggggcg gtttatcggg tggtttgttg ccggttttac ctgactgcca ccccgttcgc
     3901 ggcgaacccg tccggggcgg tgcgggcaac gggtgttatg tttaatcagc gtgaacggca
     3961 gatacaaaaa agccccggag ggcttttaac tactgactta tttttttgaa gatttgctgg
     4021 gtttcaattt gtcagtattt cccggcgttt ttttattgtc acgctggcgt ttatcaggtt
     4081 cccctgttga atcggcaatt cgtttcggac ccggcttaag acccataatc gttatccttt
     4141 caatagttaa gaggaaagcc ctcaagctag atatattgac cgcgttacgg attatcaaga
     4201 ggggctaaca agattatttt ttgttctttt aggttgattg tggtactgtt gatatacatg
     4261 tatagcaaat ggtgttcggg aggtttatat gcttgctatc cggttatctg atgagattga
     4321 gtcccgtctg gactcgctgg cgaagcaaac cggcagaaca aagacgtttt atgcgcggga
     4381 agcaatactg gcgcatctgg aagacctgga ggattattat ctttcagcag aaactgctgc
     4441 acgcgttcgc cgtggtgatg aagcagtgca ttcgtctgaa gacgtgagga agtcacttgg
     4501 tctggacgat taactattcc gatcgggcgc tcaaatcgtt acgcaagatg gacaaacaga
     4561 acgcacgacg gattgtggat tttatgagtt tacgcattgc agttgctgcc gatcctcgcc
     4621 agtcagggaa gccgctcaaa ggtgagctgg gcgagttctg gcgctatcgg gtgggagatt
     4681 atcgcgttct gtgtgagatc cgagatgacg agcttgttat ccttgccgcc acgattggac
     4741 atcgccgcga agtttacgac tgaagcccgc tatgcgggct tttttgatgc cgacaacagg
     4801 tattatggta aatactcgac taactacgtg ttctactgtt tgtcggtttt agttaaccag
     4861 caggttgtct taacgataat gtagccgtta tttttaacta ataaggtcat attgttaacg
     4921 tcaggcataa taaaagacat tatggcctca gagttatcat cagaagcagc tctctgcatt
     4981 actacttcga ttcccttttc tttgaatcta taagtatcca gttgcctatt aaacttagaa
     5041 agacctacaa ccatacttag tttgttatct ttaacagtaa gagatgcttc ctgagtacca
     5101 ttacatcggt agtaagcggt gtaaccgtca gcaaaagatc gaccggggaa tataaatgct
     5161 aggaacgcaa ttaaccaata catagttgag aatcttggta ttacacattt cactatcctc
     5221 tctttcattt ttcgttcacc atactttgtt atctgatttt taatttttct attttataaa
     5281 ccaagtctct accaaaaagg cgaaaattat cctcaaataa taataattgt ttaagaacat
     5341 ccatgaaagc agctgcttca ccctcagtta tgggagctac tctatataac atataacctg
     5401 agttaagggt ttcttttata ctttcttcaa tgctcattat aaatattagt ggattatcat
     5461 ttttcttccc ccatgggttt ctaattaatc cttcgatgta ctcaggtcct ttgcgtccta
     5521 ttgcctgcgc aatatttttt atttcatctg cgtgagggct ttgttgtaca gctttgttta
     5581 tgcctgcttg agtgat
//
