LOCUS       NZ_CP054749             5596 bp    DNA     circular CON 15-MAY-2024
DEFINITION  Klebsiella pneumoniae strain KP20194c4 plasmid pKP20194c4-p5,
            complete sequence.
ACCESSION   NZ_CP054749
VERSION     NZ_CP054749.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15186443
            Assembly: GCF_013349005.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Hu,L.M., Zhang,X.T., Zeng,X., Chen,Y., Liu,L. and Li,G.Q.
  TITLE     Genomic Characterization of a Carbapenemase-Producing, Extensively
            Drug-Resistant Klebsiella michiganensis Strain from a Renal Abscess
            Patient
  JOURNAL   Microbiol Resour Announc 11 (12), e0082522 (2022)
   PUBMED   36321904
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Zhang,X., Ouyang,J., He,W., Zeng,T., Liu,B., Jiang,H., Zhang,Y.,
            Zhou,L., Zhou,H., Liu,Z. and Liu,L.
  TITLE     Co-occurrence of Rapid Gene Gain and Loss in an Interhospital
            Outbreak of Carbapenem-Resistant Hypervirulent ST11-K64 Klebsiella
            pneumoniae
  JOURNAL   Front Microbiol 11, 579618 (2020)
   PUBMED   33281772
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5596)
  AUTHORS   Zhang,X.T. and Liu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2020) Clinical Research institute, The Second
            Affiliated Hospital, University of South China, 35 Libration Ave.,
            Hengyang 421001, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 23-JAN-2020
            Assembly Method                   :: SPAdes v. 3.14
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013349005.1-RS_2024_05_15
            Annotation Date                   :: 05/15/2024 02:46:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,918
            CDSs (total)                      :: 5,797
            Genes (coding)                    :: 5,618
            CDSs (with protein)               :: 5,618
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 68 of 179
            Pseudo Genes (incomplete)         :: 119 of 179
            Pseudo Genes (internal stop)      :: 24 of 179
            Pseudo Genes (multiple problems)  :: 32 of 179
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP054749.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP20194c4"
                     /serotype="ST11-K64"
                     /isolation_source="ventilator"
                     /db_xref="taxon:573"
                     /plasmid="pKP20194c4-p5"
                     /geo_loc_name="China: Hunan"
                     /lat_lon="27.72 N 112 E"
                     /collection_date="2019-04-15"
                     /collected_by="Xiaotuan Zhang"
     gene            complement(join(5509..5596,1..263))
                     /locus_tag="HUS04_RS29325"
                     /old_locus_tag="HUS04_29330"
     CDS             complement(join(5509..5596,1..263))
                     /locus_tag="HUS04_RS29325"
                     /old_locus_tag="HUS04_29330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(329..874)
                     /locus_tag="HUS04_RS29330"
                     /old_locus_tag="HUS04_29335"
     CDS             complement(329..874)
                     /locus_tag="HUS04_RS29330"
                     /old_locus_tag="HUS04_29335"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(900..1226)
                     /locus_tag="HUS04_RS29335"
                     /old_locus_tag="HUS04_29340"
     CDS             complement(900..1226)
                     /locus_tag="HUS04_RS29335"
                     /old_locus_tag="HUS04_29340"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            complement(1584..1742)
                     /locus_tag="HUS04_RS29920"
     CDS             complement(1584..1742)
                     /locus_tag="HUS04_RS29920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            2382..2558
                     /locus_tag="HUS04_RS29340"
                     /old_locus_tag="HUS04_29345"
     CDS             2382..2558
                     /locus_tag="HUS04_RS29340"
                     /old_locus_tag="HUS04_29345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            2548..2892
                     /locus_tag="HUS04_RS29345"
                     /old_locus_tag="HUS04_29350"
     CDS             2548..2892
                     /locus_tag="HUS04_RS29345"
                     /old_locus_tag="HUS04_29350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3101..>3535
                     /locus_tag="HUS04_RS29780"
                     /pseudo=""
     CDS             3101..>3535
                     /locus_tag="HUS04_RS29780"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3570..4079
                     /locus_tag="HUS04_RS29355"
                     /old_locus_tag="HUS04_29360"
     CDS             3570..4079
                     /locus_tag="HUS04_RS29355"
                     /old_locus_tag="HUS04_29360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            4086..4298
                     /locus_tag="HUS04_RS29360"
                     /old_locus_tag="HUS04_29365"
     CDS             4086..4298
                     /locus_tag="HUS04_RS29360"
                     /old_locus_tag="HUS04_29365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            4965..5189
                     /locus_tag="HUS04_RS29365"
                     /old_locus_tag="HUS04_29370"
     CDS             4965..5189
                     /locus_tag="HUS04_RS29365"
                     /old_locus_tag="HUS04_29370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            5173..5439
                     /locus_tag="HUS04_RS29370"
                     /old_locus_tag="HUS04_29375"
     CDS             5173..5439
                     /locus_tag="HUS04_RS29370"
                     /old_locus_tag="HUS04_29375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     Typing          2217..2296
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(1634..2031)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          2883..4414
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tcaggcataa taaaagacat tatggcctca gagttatcat cagaagcagc tctctgcatt
       61 actacttcga ttcccttttc tttgaatcta taagtatcca gttgcctatt aaacttagaa
      121 agacctacaa ccatacttag tttgttatct ttaacagtaa gagatgcttc ctgagtacca
      181 ttacatcggt agtaagcggt gtaaccgtca gcaaaagatc gaccggggaa tataaatgct
      241 aggaacgcaa ttaaccaata catagttgag aatcttggta ttacacattt cactatcctc
      301 tctttcattt ttcgttcacc atactttgtt atctgatttt taatttttct attttataaa
      361 ccaagtctct accaaaaagg cgaaaattat cctcaaataa taataattgt ttaagaacat
      421 ccatgaaagc agctgcttca ccctcagtta tgggagctac tctatataac atataacctg
      481 agttaagggt ttcttttata ctttcttcaa tgctcattat aaatattagt ggattatcat
      541 ttttcttccc ccatgggttt ctaattaatc cttcgatgta ctcaggtcct ttgcgtccta
      601 ttgcctgcgc aatatttttt atttcatctg cgtgagggct ttgttgtaca gctttgttta
      661 tgcctgcttg agtgattttc agagactcta caccgtgtat gttgtatgag ataaaatgta
      721 ataaattaag atgcaatgca attaatccat ttttgtttac atctgagtat ttggataaaa
      781 aacgtaatga agtgttaact ctcccttgaa gcctaacaat tgggagtgct gccttgtata
      841 tttccttctc cgttaaatac cgtttaaaaa acataattct ccgcctccgt atagcgtgcc
      901 tattttattt cgtcgtaaaa cttagctctg aaatattcct ttagaaccat catgtcatta
      961 ataattgaat ctatctcatc cattttttct tggtattctt ttaatggtga agggagcggt
     1021 aactcaatat ttgaaaagtg attggcgaga tagggatcaa ggtttctcat ccattcaggc
     1081 ttttcgccaa tgcgttcttc aactgaaagt gaacctgata atctctggtt aataagatca
     1141 cttagactca ttttggtttt attcgaaagc cgttgtaagg attgctttaa tcctatatct
     1201 agcatgatat ttgttctttc tctcattacc cccccttgct attgcgtttt ttgttgcgca
     1261 caaagctacc attgcgcatt aagaaacgca ataaaaaagg agggagggag tggtttgtac
     1321 atgatgtgca gtgcgctgag ggcatcagtg gcgggtgttt ttccgtggga tggatggagc
     1381 tccggggata acagaactga cgatcattta aaggatcttc ttgagatcct ttttttctgc
     1441 gcgtaatctc ttgcactgta aacgaaaaaa ccgcctaggg aggcggtttg atcgaaggtt
     1501 aagtcagttg gggaactgct taaccgggta actgggtata cgagaccgca gataccaaaa
     1561 ctgttctttc agtgtagccg cagttaggca cccacttcaa gacctctcaa tatctctctc
     1621 gtatatatcc agttaccaat ggctgctgcc agtggcgttt ttgcgtgtcc gtccgggttg
     1681 gactcaagat gatagttacc ggaaagggcg cagcagtcgg gctgaacggg gggttcgtgc
     1741 atacagccca gcttggagcg aacgacctaa accgagccga gataccagca gcgtgagcta
     1801 tgagaaagcg ccacgcttcc cgaaagggaa aaaggcggaa caggtatccg gtaaacggca
     1861 gggtcggaac aggagagcgc acgagggagc cgccggagag aaatcaccgg catctttaag
     1921 tcctgtcggg tttcgtcctc ctctgacttg agcgtccatt tctgtgatgc tcgtcagggg
     1981 aggcggagcc tatggaaaaa cggcaacgcc gcatcctttt ctccggccct ttctgatttg
     2041 agcgcctttg ctttatcccg ttatccgtga gtcggtcacg cccgccgcag ccgaacggca
     2101 ggagcgcagc taccgagtga gcgaggaggc gtcatttcat ctgtccgtgc atggtattgc
     2161 ggacgctcct gagccacata tcacacctgc cacatgaggc actttcatga atcgtgctct
     2221 catgccaaca tagtaagcca gtatacacag cgtaaacgct gtgactggtt cagggctgcg
     2281 ccccgaaacc cgctaacacc actgacgcgc tggcgcttgt ccaacgccgg agcgacccgg
     2341 aaaacttttc cgtgccgtcc ggcgtttgcg aaggagggat aatgcagaag ccgaagaaga
     2401 agccgctcgt cgtaaaaaaa agatcctggc atccggcagt gatggcaaat ctgatgcgtc
     2461 aggcacagga ggaagattac cgccgggagc agatggcaga gcttgagcgt tcagcccggt
     2521 cacattttgc ggatatcgga ggagaggatg gcggttaagc gtacccgttt tttaggtatc
     2581 cgggtgaccg atggggagta ccagcagctg ctggagcgct gcaatggaag gcaactggcg
     2641 gtgtggatgc gggaaacctg tctcgatacg cgcccggcgc ggtcgttgcg gctgccgtcc
     2701 atcgatccgg ttttgctgcg ccagcttgcc gggatgggga acaacctcaa ccagatcgcc
     2761 cggaaaatta acggcggcca gtggtcaggc gctgaccggg ttcaggtggt ggccgcgctg
     2821 atggccattg atgccgaact cgagcggctg cggcatgccg tgctggaaaa gggggcagac
     2881 gatgatcgtt aagtttcatc cgcgagggcg aggcggcggt gccgggccgg tggattatct
     2941 gctggggaaa gaccgccagc gcgacggggc catcgtcctg cagggaaagc cggaggaggt
     3001 ccgggagctt atcgacgcct cgccctacgc caaaaagtac acctccggcg tcctgtcttt
     3061 tgctgaacag gatttaccgc ccggccagcg tgaaaagctg atggcgagct tcgagcgggt
     3121 tctgatgccc ggactcgata aagaccagta cagcgtgctg tgggttgaac accgggacaa
     3181 ggggcggctg gaactgaatt ttctggtacc gaacacggaa ctgctgaccg gcaggcgtct
     3241 ccagccgtac tacgaccggg cagaccgtcc gcgcatcgat gcgtggcaga ccgtggtgaa
     3301 cgggcgactg gggctgcacg acccgaacgc accggagaac cggcgcgcgc tggtgacgcc
     3361 atcttcgtta cccaaaacga agcaggaagc cgcagaggct attacgcggg gcttactggc
     3421 cctcgcctcg tcaggggagc ttaaaacgcg tcaggacgtc actgaggcgc tggaaagcgc
     3481 aggttttgag gtcgtgcgca ccacaaagag cagcatcagc attgccgacc cggacggagg
     3541 gcgaaacatc cgacttaagg gagccatcta tgaacagtct tttaacgctg gcgaaggact
     3601 tagagcagaa atcgaaagcg cagcagcaga gtaccggcga aatgctgaaa gccgcattca
     3661 gcgagcacga gaagtctgtc agagcggaac tgagcgaaag cgggaagaga atcagcgccg
     3721 ccatccgcgc ccacgagcag ggtatgagcg cggccatgca gtcgaaccgc ctgagcgtga
     3781 tgcgtatggt cgggcggaca tggctgacca tcacgatggt gtcaggactg ctgttcgcca
     3841 gcctgagcgg ggtgttgtgg tatcagggga gcttgatagc gtcaaatctg gccgaaatcg
     3901 accggcagaa cgcagcgctg tcgaagctga acgcgaagac ctggggcgtg acgtacctgg
     3961 aagacagcaa cggacgtttt ctggtgctgc cgaaggggac ggccgtagac aggacgcaga
     4021 gctggacggt cgggaacggg cggtcgaagc agaacgcgct gagactggtg aaggagtaag
     4081 tgccgatgac cgagctggaa aaacagttgc tgagcgcatt agagcagcta cagcaggact
     4141 actcgaaaag gctggacgag tgggagagcg ccttcgggga atggcggaaa atgtcagggc
     4201 ttatgcaacg ggagaacgcg gcgctgagcg agcgcgtgac gcgcttgagt cagcaggtgg
     4261 aacgcttgag cgggcaactg cagcgtttga gccggtagtg cagcgtcatg agatggccgt
     4321 ggccgcagaa cgggcgcacg agcaacggca gcacgaaaag gaactgacgg aagcgcgttc
     4381 ccggcagcga agctatgacg gtccgtcgct gggctgaaat gcaggaaaac agggttctca
     4441 tcgggcattt acgctgaaac cggatgtcat tcacctggct gcatggctat gcagccaggt
     4501 aaaatttatc tgtcgcgaac ggggcggttt atcgggtggt ttgttgccgg ttttacctga
     4561 ctgccacccc gttcgcggcg aacccgtccg gggcggtgcg ggcaacgggt gttatgttta
     4621 atcagcgtga acggcagata caaaaaagcc ccggagggct tttaactact gacttatttt
     4681 tttgaagatt tgctgggttt caatttgtca gtatttcccg gcgttttttt attgtcacgc
     4741 tggcgtttat caggttcccc tgttgaatcg gcaattcgtt tcggacccgg cttaagaccc
     4801 ataatcgtta tcctttcaat agttaagagg aaagccctca agctagatat attgaccgcg
     4861 ttacggatta tcaagagggg ctaacaagat tattttttgt tcttttaggt tgattgtggt
     4921 actgttgata tacatgtata gcaaatggtg ttcgggaggt ttatatgctt gctatccggt
     4981 tatctgatga gattgagtcc cgtctggact cgctggcgaa gcaaaccggc agaacaaaga
     5041 cgttttatgc gcgggaagca atactggcgc atctggaaga cctggaggat tattatcttt
     5101 cagcagaaac tgctgcacgc gttcgccgtg gtgatgaagc agtgcattcg tctgaagacg
     5161 tgaggaagtc acttggtctg gacgattaac tattccgatc gggcgctcaa atcgttacgc
     5221 aagatggaca aacagaacgc acgacggatt gtggatttta tgagtttacg cattgcagtt
     5281 gctgccgatc ctcgccagtc agggaagccg ctcaaaggtg agctgggcga gttctggcgc
     5341 tatcgggtgg gagattatcg cgttctgtgt gagatccgag atgacgagct tgttatcctt
     5401 gccgccacga ttggacatcg ccgcgaagtt tacgactgaa gcccgctatg cgggcttttt
     5461 tgatgccgac aacaggtatt atggtaaata ctcgactaac tacgtgttct actgtttgtc
     5521 ggttttagtt aaccagcagg ttgtcttaac gataatgtag ccgttatttt taactaataa
     5581 ggtcatattg ttaacg
//
