LOCUS       NZ_CP054748            11971 bp    DNA     circular CON 15-MAY-2024
DEFINITION  Klebsiella pneumoniae strain KP20194c4 plasmid pKP20194c4-p4,
            complete sequence.
ACCESSION   NZ_CP054748
VERSION     NZ_CP054748.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15186443
            Assembly: GCF_013349005.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11971)
  AUTHORS   Hu,L.M., Zhang,X.T., Zeng,X., Chen,Y., Liu,L. and Li,G.Q.
  TITLE     Genomic Characterization of a Carbapenemase-Producing, Extensively
            Drug-Resistant Klebsiella michiganensis Strain from a Renal Abscess
            Patient
  JOURNAL   Microbiol Resour Announc 11 (12), e0082522 (2022)
   PUBMED   36321904
REFERENCE   2  (bases 1 to 11971)
  AUTHORS   Zhang,X., Ouyang,J., He,W., Zeng,T., Liu,B., Jiang,H., Zhang,Y.,
            Zhou,L., Zhou,H., Liu,Z. and Liu,L.
  TITLE     Co-occurrence of Rapid Gene Gain and Loss in an Interhospital
            Outbreak of Carbapenem-Resistant Hypervirulent ST11-K64 Klebsiella
            pneumoniae
  JOURNAL   Front Microbiol 11, 579618 (2020)
   PUBMED   33281772
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 11971)
  AUTHORS   Zhang,X.T. and Liu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2020) Clinical Research institute, The Second
            Affiliated Hospital, University of South China, 35 Libration Ave.,
            Hengyang 421001, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 23-JAN-2020
            Assembly Method                   :: SPAdes v. 3.14
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013349005.1-RS_2024_05_15
            Annotation Date                   :: 05/15/2024 02:46:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,918
            CDSs (total)                      :: 5,797
            Genes (coding)                    :: 5,618
            CDSs (with protein)               :: 5,618
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 68 of 179
            Pseudo Genes (incomplete)         :: 119 of 179
            Pseudo Genes (internal stop)      :: 24 of 179
            Pseudo Genes (multiple problems)  :: 32 of 179
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP054748.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11971
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP20194c4"
                     /serotype="ST11-K64"
                     /isolation_source="ventilator"
                     /db_xref="taxon:573"
                     /plasmid="pKP20194c4-p4"
                     /geo_loc_name="China: Hunan"
                     /lat_lon="27.72 N 112 E"
                     /collection_date="2019-04-15"
                     /collected_by="Xiaotuan Zhang"
     gene            818..2782
                     /locus_tag="HUS04_RS29255"
                     /old_locus_tag="HUS04_29260"
     CDS             818..2782
                     /locus_tag="HUS04_RS29255"
                     /old_locus_tag="HUS04_29260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            2782..3513
                     /gene="mobC"
                     /locus_tag="HUS04_RS29260"
                     /old_locus_tag="HUS04_29265"
     CDS             2782..3513
                     /gene="mobC"
                     /locus_tag="HUS04_RS29260"
                     /old_locus_tag="HUS04_29265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            3520..4050
                     /locus_tag="HUS04_RS29265"
                     /old_locus_tag="HUS04_29270"
     CDS             3520..4050
                     /locus_tag="HUS04_RS29265"
                     /old_locus_tag="HUS04_29270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(4077..4256)
                     /locus_tag="HUS04_RS29270"
                     /old_locus_tag="HUS04_29275"
     CDS             complement(4077..4256)
                     /locus_tag="HUS04_RS29270"
                     /old_locus_tag="HUS04_29275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(4282..4710)
                     /locus_tag="HUS04_RS29275"
                     /old_locus_tag="HUS04_29280"
     CDS             complement(4282..4710)
                     /locus_tag="HUS04_RS29275"
                     /old_locus_tag="HUS04_29280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(4874..5188)
                     /locus_tag="HUS04_RS29280"
                     /old_locus_tag="HUS04_29285"
     CDS             complement(4874..5188)
                     /locus_tag="HUS04_RS29280"
                     /old_locus_tag="HUS04_29285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(5176..5454)
                     /locus_tag="HUS04_RS29285"
                     /old_locus_tag="HUS04_29290"
     CDS             complement(5176..5454)
                     /locus_tag="HUS04_RS29285"
                     /old_locus_tag="HUS04_29290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(5629..5994)
                     /locus_tag="HUS04_RS29690"
     CDS             complement(5629..5994)
                     /locus_tag="HUS04_RS29690"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(5991..6362)
                     /gene="tolA"
                     /locus_tag="HUS04_RS29295"
                     /old_locus_tag="HUS04_29300"
     CDS             complement(5991..6362)
                     /gene="tolA"
                     /locus_tag="HUS04_RS29295"
                     /old_locus_tag="HUS04_29300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(6636..6881)
                     /locus_tag="HUS04_RS29300"
                     /old_locus_tag="HUS04_29305"
     CDS             complement(6636..6881)
                     /locus_tag="HUS04_RS29300"
                     /old_locus_tag="HUS04_29305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            7526..9013
                     /gene="ltrA"
                     /locus_tag="HUS04_RS29305"
                     /old_locus_tag="HUS04_29310"
     CDS             7526..9013
                     /gene="ltrA"
                     /locus_tag="HUS04_RS29305"
                     /old_locus_tag="HUS04_29310"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            9118..10803
                     /locus_tag="HUS04_RS29310"
                     /old_locus_tag="HUS04_29315"
     CDS             9118..10803
                     /locus_tag="HUS04_RS29310"
                     /old_locus_tag="HUS04_29315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            10813..11070
                     /locus_tag="HUS04_RS29315"
                     /old_locus_tag="HUS04_29320"
     CDS             10813..11070
                     /locus_tag="HUS04_RS29315"
                     /old_locus_tag="HUS04_29320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            11155..11304
                     /locus_tag="HUS04_RS29320"
                     /old_locus_tag="HUS04_29325"
     CDS             11155..11304
                     /locus_tag="HUS04_RS29320"
                     /old_locus_tag="HUS04_29325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     BGC             4118..11971
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(11408..11971)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.423"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          2783..3510
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 tgccgttttg cctcacatgt tagtcccctg cttatccacg gaatctgtgg gtaactttgt
       61 atgtgtccgc agcgcccgcc gcagtctcac gcccggagcg tagcgaccga gtgagcgagg
      121 aggcgcaatt tccccggcac ctgcaagacg gtcaccggac gcgctgatgt gggatattgt
      181 cccctgatta agtctttcta ttccctgacg atcaattatc aggataggtg aaccatgtcg
      241 gtttttcgct gaacatttta cgttaaccta tctctgttgc ttttttctcc ttgcggagaa
      301 atgcaagtca tccccgctgg ggcgtggctg acttcagggg aaaagacctt gcttacgctg
      361 gctggacgcc accgacgcac ggtcttttcc cctctttctg gctctgtccg gctggacgcc
      421 ggccacgcca gaaattcacc cggcccccgt caggctcacg cctgaccacc cacaaaaatc
      481 cacggtgctg gacgcacctg acgaagcagc aaagctgctc ctgattttgc gggtggtcag
      541 ccgctcgcta tggcgaactc tgacgggggc cggaacaccg gaaaagaccg cactggggtg
      601 ccgccttttc ctctcacacg ctggggcgtg ctcgttgttc ctccggggct gcgccttttc
      661 cgcatggggc ggaaagaggc tggcctccgc ggtgaaagct gaacgcaatc accacggacg
      721 tgaccgccgc cgccatgcct tggggcatgt ccgcggcttg tgcagcgggg gctgcgcatc
      781 acgccggtaa acccgtcgtc tgaaggagag agattgcatg tttaatacgg attcgcttgc
      841 ctggcagtgg catatcccgg agctggcccg gcagatttac ccgtgggtca gctggctgta
      901 ccgggagtat tacagtctgg ccggtttttt tctggttatc ggcctgtttt tcggtctggc
      961 tgaactctgg gccggccgaa tgcgttttgt ggggctgagc cataaggcgc ggcgggagta
     1021 tgccgatcgc tataagcgcg ataataaccg gactgtgcca ttttcggtgc tggcctcgct
     1081 ggattcaccc gggcggatgg tcagggcacg gcgacatgtc tggaactacg cgtactggct
     1141 gaccggcgct gccggtctgg cccttctggg ggggctggtt gtcgccgtgt ttatcgagag
     1201 ctggggagaa accgtcctgc gcccggacct ctatgcgaac gcagattatt acctgctgca
     1261 gccggctaac ctgttcggga tagcctggtg tgccggaggg cttgtcgccg gcgtccttct
     1321 ggcgcggctg ctggcgttcc tgctgcttga tggtcatttt gcggccgctg acgaggcggt
     1381 gaacgcgaaa ctgaatcagg agagccggcg cagcagccag cggaccggag aaatgacaga
     1441 cgtccgccac ctgcatttcg gggagccggt accggttaac gcgctggccg atttttccac
     1501 ggagcaggcc cggaaacagc aggcggtgtt tctgggtaag gatgagcagg gccagccggt
     1561 gctggtccct cgtgatacct ggcgcaagac caatattcag atcctcggcc tgcccgggag
     1621 cggcaagagc gcgatggcca ccaacgcgct cattcgctgc gtacgcgatt tcggggacgc
     1681 tgtggtgtac ttcgacccca aaggggacgc gtgggcgccg cacgtcttcc gggcgcactg
     1741 cccggacttc accctgctgg atctgcggcc ggggaaaccg gcgcagctga atctgttccg
     1801 cgatctcgac cagtacgccc tgaagaatct gctggttgcc gggtttaacc tgagtgagac
     1861 cggcgacgtg gcggaccact accgcatcag cgagcagaaa gcggcgaagc tgattgcgga
     1921 gcagttcccg cagggggcaa acattcagca ggtgctggcg gcggcgtacg cgttgccgga
     1981 ggccctgaaa aaggacgtga aggggctgat caccaagctg gagaacgtgg ccgacctgag
     2041 cgtcctgcag acggacagcg gtatcgatgt ggcggggata atcaacggcg gcggctgcct
     2101 gtacgtcatc ggctcgatgg acgatgaggc ggtgatccgg gtgcagaaga tgctgtttgc
     2161 ccgctgcgcg caaatcatca ttgcgcggga tgagttccgg aggtggccgc acgcgagcat
     2221 catgctcgat gagattaaat atctgctgtc gaagtacgtg ctgaacgcgc tggggacgct
     2281 gcgcagccgg gactgcaacc tgctgctcgc gcaccagtcg ctgggcgact tcggtcagtg
     2341 cgggcaggat ctgcccgctg attttgtgaa aaccacggta ctggataaca cgccgatccg
     2401 ctggttctat cgcgcggcca gccaggagtc cgcgcagtgg gcggcgggcc agaccgggga
     2461 gatccgggtg gacgtggagc gccgccgggc cagccgggag gcggggaacg tggagcacat
     2521 cagcggcgac agcttcatcc agaaggaggc gcggccgctg tttgacgtga acaccctgca
     2581 acacctgcct gacgggttcg cggtgatgac cgggctgggc gtggcgcggc tggcgttcag
     2641 cagcccgctg cgggttgagc gccgggaaat ttcgctgaaa accttcccgg tgctggcgaa
     2701 aacggacccg ctggcggagt acctgcctcc ggccgacggc ggcggaccaa aagcggatga
     2761 tgattttgcg gggctttact gatgacactg gagcagtaca acgtcagcca cgcgaagcgg
     2821 caggcgcgga acgcggagaa aacacggctg accctgcgct ggctgcggga agagctgtgt
     2881 tccacggcgg agctggttgc gcgacggctg gggattgcgg cggtacagcc ggtttaccgc
     2941 ttcctggaca gtctggtggc gaaagggctg ctggtccggg cgaaataccc ggttgacggc
     3001 cgtcaggtca gcgtgtgggg gctgacgccg cacggggtgg cgttctcgtt cgacgaggac
     3061 gagccgctga cggacgttat tccgtttcag ccgtcaaggg tgtcggcggc gcagctgccg
     3121 caccggctgg ccgtgcagtc cctgcgcctc tccatggagg gccggggggc caccggctgg
     3181 cggtacctgc accgtatggc cctgaaaggg atgaaagtgc cggacgcgct ggcggagctg
     3241 gacggcagaa cggtggcatt tgaggtagag cgcaccgtca agagccgcag gcggtatcag
     3301 gaagtggtgt ccgggtatct gttcaaccgc cgggcgaacg gcatcgatga aatctggtac
     3361 atctgccctg accggccgac gcttgtccgg gtacagcggg cgattctggc ggtggatgaa
     3421 atcgtgaatc cacagaccgg cgaggcccgc aaaacggtgg agctggaccg ggagaggctg
     3481 ttcgcgtgct ttaaatttat gacaacggag tgagggatta tggcgctgta catggtattt
     3541 attgaggacg tggaagacaa agacggcgag cgctgcatta tgccaaccag cgagcatctg
     3601 gaagacatgg tggtgctcgc ctggcagacc ggggcgtatg ttttctgttt tgtcgaccgg
     3661 gcccagcatc tggcgatgat gaaatccatc gggatgtatg acgagcggct caacagcgac
     3721 gaaaaagcca cgtacttttt caccggtgaa aatccgcaat gggaacggga caaacccaca
     3781 ggaatgaagc tgtgtgatta cctctatcga aaggtggagc ggttcgccat cgagaacggt
     3841 gtgcaggtgg ttgatgccgg gaagtgctca ccggagcaga ttaacgcgat gattgaggtg
     3901 gtcagcgagc ggcacgggga aagcgcggtg aacgagaagc tgaaagcgga gctgatgcgg
     3961 gaatataaca gccgctattt taacgtgccg gtgcctgaac tgccggagga caaacccgcc
     4021 cgccggtggt ggccgttcgg caaaaaataa cgccagcacc ggcatggccg gtgctgttat
     4081 tcttccccga gcttatcccg catcacctgc cagagtgttt ccgcatcctc gtgaagccgt
     4141 tcgcacatgt cggccggggt atccatgtcc agctcgttca gcttttccag caacagcagc
     4201 gactgatcct taatgaattt agccatattg agggcagttt tttcctgctt cgtcatgttc
     4261 tccattccct tcggtaagtt ctcagctaaa ccagccggac cagaagcgtt ttttcttctg
     4321 tgtcagttct gttgtcaatc tgtccacttc ggcctggagt ttccggcgct catcatcgtg
     4381 ctggcggcgt tcttcttccc gggatgtttt atcttccagc atcagggtga cagcctgccg
     4441 gagatcggcc agttcctttt gcattgccag aagctgttcc tgtgggaaat ttttttgtgt
     4501 attttcacgg tgtgaaatgc cgggaatttg tttctcaggg atattcacat ttccgtatac
     4561 acggatcagt tctgacacat ccacgacagg gttgtttttc ccgtcacgtg acacggtaac
     4621 ctttccctgt tttacatgat tgtatagcgt tcttctggta atgcccgcgg cttgtgctgc
     4681 ctgtgaaagg ttgagcaatg ttttcgccac tgtatacccc tgattttatc gggtgtgaaa
     4741 ggtgtgtaat ttctcacggt aaatgtatcg ctgaatttta cccgtttttc cgttcaggat
     4801 gtggttttct gacgcgctgc gcttgtcaaa ccggcgacgg ccacgcaatg aattgcgccc
     4861 ccgtcatcgg ttattagagg ctgtctgccc ccggataccc ggattctttc tgatgcctga
     4921 cggtgacgat cacaatctgg tccatttccc ggtcgtggcg gtagagcatc acatagccac
     4981 tgtcgccaaa cccgatcacc agttccttat attccagcgg cagaaacggc accgggcggc
     5041 caatgtctgg caatgttttc agttgctgga tagcccggac gatcacctca cctccttttc
     5101 tggctgccag ccgatttttg gttttgagaa agtcctgaag gcgttgtaaa tcgcgctgtg
     5161 ccagcgcgga aatcgttacc tgtggcatgg cggagcatcc tgctcgttct cagttcccca
     5221 ggtacttatc caggcttccg cttcttccgc agtaaggtgc agacccgttt cctgatagtg
     5281 ctgccatgcg gcctgtccgt cccgcaggta ctggctgcgc ttctcttccc ggtcgatgta
     5341 ttcggtgatt gcctccagca ttagcgcgtg cgctgagcgg tgccggtcat cggccagcgt
     5401 tttcagccgg tccttaagtt cttcatcgag cctgatggat gttgctgatg acataaaagc
     5461 ccctttgtag tcatatgtat tacacatgac tactttaatc cgtggcggcg ctatttgcca
     5521 gaaatacggc ttttgctgcc catttttcag cttcgcttcc actaatcagg gctgctacag
     5581 gctcactcat catccaggac tattgcagcc tgcgggttaa aggacctgtt actggcggca
     5641 accatcatca ttgagctttt gggtggtgac tggtcgcgga tcctgtaagc cgtcattttt
     5701 cagactgaat tcaaaattta taacctgtcg cattgccggg tgccctgcgg gatatagcca
     5761 gtgcctcatt gccgtcagcg tttcccgaaa aaaaaggtga cagccggtaa gcgacacgtt
     5821 gtccactcgt ccatcgtcca gaatatcaaa cgtggcggtc acctgacctt cgatgcgttt
     5881 tgctgcggcc tgtaccgggt aggttggctg cagtgtggtt acgggtttta gtggtatcgc
     5941 atgtaccgga gtgaggaaca tggccgccag cactgtcaga gtaaaagctt tcatggtctg
     6001 aaatccagag gggcgttttt gaatatttgc caggtatttt catccggtgc cggcggaatt
     6061 ttggcgtggg taacggctga tattgctgct ttacagagtt tcggatctcc tccttcagca
     6121 atggcgctgt tcagagtgcc atcacgttgt atggatatcc gcacggtgca catttttccc
     6181 gcataggcct ctggttcaaa catctgcatt tcaacagcgg tcttgatggc cgcagcgtag
     6241 ctactgattt tagatgactt atccagagta taaattttcg gcggctgggt ggtggaatgc
     6301 ttacaggccg tcaggagtaa ggggctgcac aatgccagca ggataaaggg tattaaacgc
     6361 atcgggttga tgacctgtca gtaaaaaatg ttacatgtat atttgtcact ctttacggcc
     6421 acgttcattt acataaacac ggcttatcgt atcgttttta caccaaacgg cccgccatgc
     6481 agttgcgtgc ataggcatgc agcgggttac aatttaccgg gcgcgtttcc ggcggttttc
     6541 cgggtggttt gttgcctgtt ttatcccgta gccgccggaa acgccctcag accgtctgag
     6601 cggtgcgggc aatgcgacgt tatggtaaat accccttacc ggcggcgctg tggctgccag
     6661 gtgaaggtgg tttccgtagc ctcgtatttc tcccgacgca tttcctgcgc ccgcctggct
     6721 tcacgttcgt cctgaatggc acgaagcacc ctttcccgca cctgttgttt taccgccttt
     6781 ggcagcgttt tgttcccttt caggcccatg gccagtgcct gagcgacaaa ggcgtccgcc
     6841 aggcgctttt cttcagcggg agtcagtttc aggtgcgtca tagtggcctc cgggcgtctc
     6901 aggtttcccg aacagtttaa ccgcaggtcc tctcctcatg ccagttagct ttcagatgtg
     6961 tctataccat ctgaaatgta agtgtgatga atgtcttaaa taataaaatt tgttgccatg
     7021 agaaattttc gggaaaagac gcgatctgct tacaggtggc tccgggcgct tttttgtgcc
     7081 tgaagatgag aactaatctc atatattgac acctgaaaac gggaggagta aggtaataat
     7141 tatactgtgt atatgtacag tgtgcgccgg gagaccggtt agagatcaag gggtgaaagt
     7201 ccccgaccat tgaaggacca gcaatccaca aggtccccga gtcatgcgtt gcataccgcg
     7261 aggtatgggg cgaagcgttg acaggggtgt tgacaggcca gccattgagc cacgaaatgt
     7321 atattaaatt accgggtgcc gacgttgtac tgttaacgga aggcaacatc atagggtgcg
     7381 acactgcgag tgccacatgg acccggcggg gtctgagacc ctggcatgtc aatacgatct
     7441 ctacgcggga accgggagat ctcccctctg accatctgcc agtgtcggag atggcccgca
     7501 ccgggaagac gaggagtcat agccggtgat gtacggagag gagaagtcgg actcgctcat
     7561 agtagcggcg aagcaggcga acaacccgaa aggagcggag tcagtggagc gaaggagcgg
     7621 ggccaagggg aacgcggaac agccacacat gcgccggaca cagagccggg aaagcatgtc
     7681 acagaggctg tcacgcgtgc gggaagctgc gaagcagcgg aagaaagaac ggtttacagc
     7741 attgttccac ctgctgacag tcgaagcact ggaagccgca ttcctctccc tgagcaggaa
     7801 agcggccgcc ggagtggatg gcatcaggtg gatggactac gccggaaaca tgaagaacaa
     7861 cataacagat ctgcaccgga ggctacatca gggcagctac agggcgcagc ccggcaggcg
     7921 tcactacatc ccaaaagcgg atggaaaaca acgcccgctc ggcatcgcct cgctggagga
     7981 caagatcgtc cagtatgcgc tggtgaaaat cctgaacgca gtctatgaaa acgactttat
     8041 ggggttctca tacgggttca gacccgggcg aagccagcac gatgcactgg acgcactggc
     8101 cacagggctg gtacgcacta acgtaaactg ggtactggat gccgacatca gtcagttctt
     8161 cgacagggtg agccacgaat ggctgatcag gttcacagag catcggatcg gcgaccggag
     8221 ggtaatcagg ctcatacgta agtggctcac agccgggacg tcggaggagg gtcaatggcg
     8281 agcaacggag gaaggcaccc cacagggtgc ggtcatctca ccgctgctgg caaacatata
     8341 cctccactac gtcttcgatc tgtgggcgca tcagtggcga cgtcgctatg ccacaggcaa
     8401 tgtggtaatg gtcagatacg ccgatgacat cgtcatcggg ttcgacaaac gatacgatgc
     8461 ccggcgcttc cgtatagcca tgcagcgcag actgagggag ttcggactca cggttcaccc
     8521 ggagaaaacc cgtctgatgg agttcggccg cttcgctgcc gaaaaccgtg ccatcagggg
     8581 aaaaggcaaa ccagaaacgt tcaacttcct cgggttcacg cacatcagcg ggaaagatcg
     8641 caacggcagg ttcatgctga tacgaaagac ccgccgggat cggatgacgg caactctgaa
     8701 agccatcaaa gacggtctgc gaaggcgctg gcattactca atccccgaac agggaaaatg
     8761 gctcaggaga gtggttcagg gatacctgaa ctatcactcg gtaccgggca acttccccac
     8821 catgcagaag ttcaggacac acgtaacaaa cctctggcgc cgggcgctca ggcgcaggag
     8881 ccagaaggat gatacgacct ggacgaaagc aaacaaactg gcagccgcat ggctaccaag
     8941 ggttcgggtt cttcatccat ggcctgtgga gcggttcacc gccagacacc cgaggcagga
     9001 gcccggtgcg taaatcgcgc acgccgggat ctgtgcgggg ggtatccggt aacgggtatc
     9061 cctaccgcga catgttggct ttaatttatt gttttaaatg tcaaagagga aaacggtatg
     9121 agtggtggag acggtcgagg tccgggtaat tcaggtctgg gacataatgg tggtcaggcc
     9181 agtgggaatg tgaacggtac gtctggtaaa ggtggccctt catcaggtgg tggtacggat
     9241 ccaaacagcg ggccgggctg gggtacgacg catacgccga atggagatat tcataactat
     9301 aatccggggg agtttggtaa cggcgggagt aaacccggtg gtaatggtgg taacagcggc
     9361 aatcatagcg gtagctctgg tggcggacag tcttcggcca ctgcgatggc cttcggtctg
     9421 cctgccctgg ctactccggg ggctgaagga ctggctttat cagtttccgg cgatgcgttg
     9481 tcggccgccg ttgctgatgt gctggctgcc ctgaaggggc cgtttaagtt tggtctgtgg
     9541 gggattgcga tctacggtgt gctgccttct gagattgcaa aagatgatcc gaaaatgatg
     9601 tcaaaaatta tgacgtcatt accggccgat gcggtgacgg agactccggc aagtacttta
     9661 ccactggacc aggcgacggt tcgtgtcaga caacgggttg tggatgtggt gaaggatgag
     9721 cggcagcata ttgcggttgt cgcaggtcgg ccaatgagtg tccctgtggt ggatgcgaaa
     9781 ccgacaaaac gtccgggggt attcagtgtg tcgattccgg gtctcccgtc tctgcaggtg
     9841 agcgtaccga aaggtgttcc gacagcgaaa gccccgccaa aaggcattgt tgctgaaaaa
     9901 ggtgattcac gtccggctgg ttttacagcc ggtggtaact cccgtgaggc cgttattcgt
     9961 ttcccgaaag agaccggaca gaagccggtt tatgtgtcgg tgacagatgt tcttaccccg
    10021 gcacaggtaa aacagcgtca ggaggaagaa aagcgtcgcc agcaggcatg ggacgccgct
    10081 catccggaag aggggctgaa aagagactat gataaagcga aagctgagct ggatgccgaa
    10141 gataaaaata ttgcgacctt aaacagccgc attgcatcga cagagaaggc gctccccggt
    10201 gcaagggctg ctgttcagga agccgataaa aaggtaaaag aggcagaggc aaataaagat
    10261 gattttgtga cttataaccc gcctcatgaa tatggctccg ggtggcagga tcaggttcgc
    10321 tatcttgata aggatattca gaatcagaat gagaaattaa aagcggctca ggcatcttta
    10381 aacgcaatga atgaatcctt atccagagat aaggctgcac ttaccggggc gatggagagc
    10441 cggaaacaaa aggagaaaaa agcgaaggat gcagaaaata agttaaatga ggaaaagaac
    10501 aagcctcgca aggggactaa agactacggc catgattatc atccagtacc taaaacagaa
    10561 gacataaagg gacttggaga gttaaagcga ggtgatccta aaacacctaa acaaggtggt
    10621 ggtggtaagc gggcccgatg gtatggcgat aaaaaacgta aaatttatga atgggattcc
    10681 cagcacggtg agcttgaagg gtatcgtgcc agtgatggcg aacacctagg cgcatttgat
    10741 cccaaaacgg gtaagcaggt taaagggcca gatccaaaac ggaacattaa aaaatatctt
    10801 tgagaggtaa atatggggct taaattacgt ttgacatggt ttgataagaa aactgaagag
    10861 tttaaagggg aagagtattc taaagacttt ggtgacgatg gttctgtcat tgaaagcttg
    10921 ggaatgcctt taaaggataa tattaataat ggtgagtttg atttgaagaa agaatgggtt
    10981 cctttactac agccattttt taaaaatcaa ataaaacctg atgtggataa ttgttatata
    11041 gcttttgatt atcgggacgg taactggtaa caaaaaatta tcaaggatga tttgaaatac
    11101 tgacttccgg tgttcaggga tgaatgccgg agtctttaaa tacagggaaa gtatatgaaa
    11161 aaggtaaaga ctatttttct ttttatattg attgttgctg gctttcttct ggccgcatgt
    11221 caggcaaatt atatccggga tgttcagggc ggaacggtgg caccatcgtc ctcctctgaa
    11281 ctgacgggga tcgcggttca gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
    11341 tgcgcgtaat cttttgccct gtaaacgaaa aaaccacctg gggaggtggt ttgatcgaag
    11401 gttaagtcag ttggggaact gcttaaccgg gtaactggct ttcgagagca cagcaaccaa
    11461 atctgtcctt ccagtgtagc cggactttgg cgcacacttc aagagcaacc gcgtgtttag
    11521 ctaaacaaat cctctgcgaa ctcccagtta ccaatggctg ctgccagtgg cgtttttgcg
    11581 tgtccgtccg ggttggactc aagatgatag ttaccggaaa gggcgcagca gtcgggctga
    11641 acggggggtt cgtgcataca gcccagcttg gagcgaacga cctaaaccga gccgagatac
    11701 cagcagcgtg agctatgaga aagcgccacg cttcccgaaa gggagaacgg cggaacaggt
    11761 atccggtaaa cggcagggtc ggaacaggag agcgcaagag ggagcgacct accggaaacg
    11821 gtggggatct ttaagtcctg tcgggtttcg cccgtactgt cagattcatg gttgagcctc
    11881 acggctccca cagatgcacc gaaaaagcgt ctgtttatgt gaactctggc aggagggcgg
    11941 agcctatgga aaaacgccac cggcgcggcc c
//
