LOCUS       NZ_CP054730            11970 bp    DNA     circular CON 16-MAY-2024
DEFINITION  Klebsiella pneumoniae strain KP20194e plasmid pKP20194e-p4, complete
            sequence.
ACCESSION   NZ_CP054730
VERSION     NZ_CP054730.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15186446
            Assembly: GCF_013348965.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11970)
  AUTHORS   Hu,L.M., Zhang,X.T., Zeng,X., Chen,Y., Liu,L. and Li,G.Q.
  TITLE     Genomic Characterization of a Carbapenemase-Producing, Extensively
            Drug-Resistant Klebsiella michiganensis Strain from a Renal Abscess
            Patient
  JOURNAL   Microbiol Resour Announc 11 (12), e0082522 (2022)
   PUBMED   36321904
REFERENCE   2  (bases 1 to 11970)
  AUTHORS   Zhang,X., Ouyang,J., He,W., Zeng,T., Liu,B., Jiang,H., Zhang,Y.,
            Zhou,L., Zhou,H., Liu,Z. and Liu,L.
  TITLE     Co-occurrence of Rapid Gene Gain and Loss in an Interhospital
            Outbreak of Carbapenem-Resistant Hypervirulent ST11-K64 Klebsiella
            pneumoniae
  JOURNAL   Front Microbiol 11, 579618 (2020)
   PUBMED   33281772
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 11970)
  AUTHORS   Zhang,X.T. and Liu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2020) Clinical Research institute, The Second
            Affiliated Hospital, University of South China, 35 Libration Ave.,
            Hengyang 421001, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 10-APR-2020
            Assembly Method                   :: SPAdes v. 3.14
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013348965.1-RS_2024_05_16
            Annotation Date                   :: 05/16/2024 02:50:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,917
            CDSs (total)                      :: 5,796
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 69 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 24 of 181
            Pseudo Genes (multiple problems)  :: 32 of 181
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP054730.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11970
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP20194e"
                     /serotype="ST11-K64"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP20194e-p4"
                     /geo_loc_name="China: Hunan"
                     /lat_lon="27.72 N 112 E"
                     /collection_date="2019-04-25"
                     /collected_by="Xiaotuan Zhang"
     gene            join(11336..11970,1..1330)
                     /locus_tag="HUS07_RS29255"
                     /old_locus_tag="HUS07_29260"
     CDS             join(11336..11970,1..1330)
                     /locus_tag="HUS07_RS29255"
                     /old_locus_tag="HUS07_29260"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            1330..2061
                     /gene="mobC"
                     /locus_tag="HUS07_RS29260"
                     /old_locus_tag="HUS07_29265"
     CDS             1330..2061
                     /gene="mobC"
                     /locus_tag="HUS07_RS29260"
                     /old_locus_tag="HUS07_29265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            2068..2598
                     /locus_tag="HUS07_RS29265"
                     /old_locus_tag="HUS07_29270"
     CDS             2068..2598
                     /locus_tag="HUS07_RS29265"
                     /old_locus_tag="HUS07_29270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(2625..2804)
                     /locus_tag="HUS07_RS29270"
                     /old_locus_tag="HUS07_29275"
     CDS             complement(2625..2804)
                     /locus_tag="HUS07_RS29270"
                     /old_locus_tag="HUS07_29275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2830..3258)
                     /locus_tag="HUS07_RS29275"
                     /old_locus_tag="HUS07_29280"
     CDS             complement(2830..3258)
                     /locus_tag="HUS07_RS29275"
                     /old_locus_tag="HUS07_29280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(3422..3736)
                     /locus_tag="HUS07_RS29280"
                     /old_locus_tag="HUS07_29285"
     CDS             complement(3422..3736)
                     /locus_tag="HUS07_RS29280"
                     /old_locus_tag="HUS07_29285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(3724..4002)
                     /locus_tag="HUS07_RS29285"
                     /old_locus_tag="HUS07_29290"
     CDS             complement(3724..4002)
                     /locus_tag="HUS07_RS29285"
                     /old_locus_tag="HUS07_29290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(4177..4542)
                     /locus_tag="HUS07_RS29695"
     CDS             complement(4177..4542)
                     /locus_tag="HUS07_RS29695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(4539..4910)
                     /gene="tolA"
                     /locus_tag="HUS07_RS29295"
                     /old_locus_tag="HUS07_29300"
     CDS             complement(4539..4910)
                     /gene="tolA"
                     /locus_tag="HUS07_RS29295"
                     /old_locus_tag="HUS07_29300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(5184..5429)
                     /locus_tag="HUS07_RS29300"
                     /old_locus_tag="HUS07_29305"
     CDS             complement(5184..5429)
                     /locus_tag="HUS07_RS29300"
                     /old_locus_tag="HUS07_29305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            6074..7561
                     /gene="ltrA"
                     /locus_tag="HUS07_RS29305"
                     /old_locus_tag="HUS07_29310"
     CDS             6074..7561
                     /gene="ltrA"
                     /locus_tag="HUS07_RS29305"
                     /old_locus_tag="HUS07_29310"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            7666..9351
                     /locus_tag="HUS07_RS29310"
                     /old_locus_tag="HUS07_29315"
     CDS             7666..9351
                     /locus_tag="HUS07_RS29310"
                     /old_locus_tag="HUS07_29315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            9361..9618
                     /locus_tag="HUS07_RS29315"
                     /old_locus_tag="HUS07_29320"
     CDS             9361..9618
                     /locus_tag="HUS07_RS29315"
                     /old_locus_tag="HUS07_29320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            9703..9852
                     /locus_tag="HUS07_RS29320"
                     /old_locus_tag="HUS07_29325"
     CDS             9703..9852
                     /locus_tag="HUS07_RS29320"
                     /old_locus_tag="HUS07_29325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     BGC             2666..11970
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(9956..10534)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1331..2058
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 gcatttcggg gagccggtac cggttaacgc gctggccgat ttttccacgg agcaggcccg
       61 gaaacagcag gcggtgtttc tgggtaagga tgagcagggc cagccggtgc tggtccctcg
      121 tgatacctgg cgcaagacca atattcagat cctcggcctg cccgggagcg gcaagagcgc
      181 gatggccacc aacgcgctca ttcgctgcgt acgcgatttc ggggacgctg tggtgtactt
      241 cgaccccaaa ggggacgcgt gggcgccgca cgtcttccgg gcgcactgcc cggacttcac
      301 cctgctggat ctgcggccgg ggaaaccggc gcagctgaat ctgttccgcg atctcgacca
      361 gtacgccctg aagaatctgc tggttgccgg gtttaacctg agtgagaccg gcgacgtggc
      421 ggaccactac cgcatcagcg agcagaaagc ggcgaagctg attgcggagc agttcccgca
      481 gggggcaaac attcagcagg tgctggcggc ggcgtacgcg ttgccggagg ccctgaaaaa
      541 ggacgtgaag gggctgatca ccaagctgga gaacgtggcc gacctgagcg tcctgcagac
      601 ggacagcggt atcgatgtgg cggggataat caacggcggc ggctgcctgt acgtcatcgg
      661 ctcgatggac gatgaggcgg tgatccgggt gcagaagatg ctgtttgccc gctgcgcgca
      721 aatcatcatt gcgcgggatg agttccggag gtggccgcac gcgagcatca tgctcgatga
      781 gattaaatat ctgctgtcga agtacgtgct gaacgcgctg gggacgctgc gcagccggga
      841 ctgcaacctg ctgctcgcgc accagtcgct gggcgacttc ggtcagtgcg ggcaggatct
      901 gcccgctgat tttgtgaaaa ccacggtact ggataacacg ccgatccgct ggttctatcg
      961 cgcggccagc caggagtccg cgcagtgggc ggcgggccag accggggaga tccgggtgga
     1021 cgtggagcgc cgccgggcca gccgggaggc ggggaacgtg gagcacatca gcggcgacag
     1081 cttcatccag aaggaggcgc ggccgctgtt tgacgtgaac accctgcaac acctgcctga
     1141 cgggttcgcg gtgatgaccg ggctgggcgt ggcgcggctg gcgttcagca gcccgctgcg
     1201 ggttgagcgc cgggaaattt cgctgaaaac cttcccggtg ctggcgaaaa cggacccgct
     1261 ggcggagtac ctgcctccgg ccgacggcgg cggaccaaaa gcggatgatg attttgcggg
     1321 gctttactga tgacactgga gcagtacaac gtcagccacg cgaagcggca ggcgcggaac
     1381 gcggagaaaa cacggctgac cctgcgctgg ctgcgggaag agctgtgttc cacggcggag
     1441 ctggttgcgc gacggctggg gattgcggcg gtacagccgg tttaccgctt cctggacagt
     1501 ctggtggcga aagggctgct ggtccgggcg aaatacccgg ttgacggccg tcaggtcagc
     1561 gtgtgggggc tgacgccgca cggggtggcg ttctcgttcg acgaggacga gccgctgacg
     1621 gacgttattc cgtttcagcc gtcaagggtg tcggcggcgc agctgccgca ccggctggcc
     1681 gtgcagtccc tgcgcctctc catggagggc cggggggcca ccggctggcg gtacctgcac
     1741 cgtatggccc tgaaagggat gaaagtgccg gacgcgctgg cggagctgga cggcagaacg
     1801 gtggcatttg aggtagagcg caccgtcaag agccgcaggc ggtatcagga agtggtgtcc
     1861 gggtatctgt tcaaccgccg ggcgaacggc atcgatgaaa tctggtacat ctgccctgac
     1921 cggccgacgc ttgtccgggt acagcgggcg attctggcgg tggatgaaat cgtgaatcca
     1981 cagaccggcg aggcccgcaa aacggtggag ctggaccggg agaggctgtt cgcgtgcttt
     2041 aaatttatga caacggagtg agggattatg gcgctgtaca tggtatttat tgaggacgtg
     2101 gaagacaaag acggcgagcg ctgcattatg ccaaccagcg agcatctgga agacatggtg
     2161 gtgctcgcct ggcagaccgg ggcgtatgtt ttctgttttg tcgaccgggc ccagcatctg
     2221 gcgatgatga aatccatcgg gatgtatgac gagcggctca acagcgacga aaaagccacg
     2281 tactttttca ccggtgaaaa tccgcaatgg gaacgggaca aacccacagg aatgaagctg
     2341 tgtgattacc tctatcgaaa ggtggagcgg ttcgccatcg agaacggtgt gcaggtggtt
     2401 gatgccggga agtgctcacc ggagcagatt aacgcgatga ttgaggtggt cagcgagcgg
     2461 cacggggaaa gcgcggtgaa cgagaagctg aaagcggagc tgatgcggga atataacagc
     2521 cgctatttta acgtgccggt gcctgaactg ccggaggaca aacccgcccg ccggtggtgg
     2581 ccgttcggca aaaaataacg ccagcaccgg catggccggt gctgttattc ttccccgagc
     2641 ttatcccgca tcacctgcca gagtgtttcc gcatcctcgt gaagccgttc gcacatgtcg
     2701 gccggggtat ccatgtccag ctcgttcagc ttttccagca acagcagcga ctgatcctta
     2761 atgaatttag ccatattgag ggcagttttt tcctgcttcg tcatgttctc cattcccttc
     2821 ggtaagttct cagctaaacc agccggacca gaagcgtttt ttcttctgtg tcagttctgt
     2881 tgtcaatctg tccacttcgg cctggagttt ccggcgctca tcatcgtgct ggcggcgttc
     2941 ttcttcccgg gatgttttat cttccagcat cagggtgaca gcctgccgga gatcggccag
     3001 ttccttttgc attgccagaa gctgttcctg tgggaaattt ttttgtgtat tttcacggtg
     3061 tgaaatgccg ggaatttgtt tctcagggat attcacattt ccgtatacac ggatcagttc
     3121 tgacacatcc acgacagggt tgtttttccc gtcacgtgac acggtaacct ttccctgttt
     3181 tacatgattg tatagcgttc ttctggtaat gcccgcggct tgtgctgcct gtgaaaggtt
     3241 gagcaatgtt ttcgccactg tatacccctg attttatcgg gtgtgaaagg tgtgtaattt
     3301 ctcacggtaa atgtatcgct gaattttacc cgtttttccg ttcaggatgt ggttttctga
     3361 cgcgctgcgc ttgtcaaacc ggcgacggcc acgcaatgaa ttgcgccccc gtcatcggtt
     3421 attagaggct gtctgccccc ggatacccgg attctttctg atgcctgacg gtgacgatca
     3481 caatctggtc catttcccgg tcgtggcggt agagcatcac atagccactg tcgccaaacc
     3541 cgatcaccag ttccttatat tccagcggca gaaacggcac cgggcggcca atgtctggca
     3601 atgttttcag ttgctggata gcccggacga tcacctcacc tccttttctg gctgccagcc
     3661 gatttttggt tttgagaaag tcctgaaggc gttgtaaatc gcgctgtgcc agcgcggaaa
     3721 tcgttacctg tggcatggcg gagcatcctg ctcgttctca gttccccagg tacttatcca
     3781 ggcttccgct tcttccgcag taaggtgcag acccgtttcc tgatagtgct gccatgcggc
     3841 ctgtccgtcc cgcaggtact ggctgcgctt ctcttcccgg tcgatgtatt cggtgattgc
     3901 ctccagcatt agcgcgtgcg ctgagcggtg ccggtcatcg gccagcgttt tcagccggtc
     3961 cttaagttct tcatcgagcc tgatggatgt tgctgatgac ataaaagccc ctttgtagtc
     4021 atatgtatta cacatgacta ctttaatccg tggcggcgct atttgccaga aatacggctt
     4081 ttgctgccca tttttcagct tcgcttccac taatcagggc tgctacaggc tcactcatca
     4141 tccaggacta ttgcagcctg cgggttaaag gacctgttac tggcggcaac catcatcatt
     4201 gagcttttgg gtggtgactg gtcgcggatc ctgtaagccg tcatttttca gactgaattc
     4261 aaaatttata acctgtcgca ttgccgggtg ccctgcggga tatagccagt gcctcattgc
     4321 cgtcagcgtt tcccgaaaaa aaaggtgaca gccggtaagc gacacgttgt ccactcgtcc
     4381 atcgtccaga atatcaaacg tggcggtcac ctgaccttcg atgcgttttg ctgcggcctg
     4441 taccgggtag gttggctgca gtgtggttac gggttttagt ggtatcgcat gtaccggagt
     4501 gaggaacatg gccgccagca ctgtcagagt aaaagctttc atggtctgaa atccagaggg
     4561 gcgtttttga atatttgcca ggtattttca tccggtgccg gcggaatttt ggcgtgggta
     4621 acggctgata ttgctgcttt acagagtttc ggatctcctc cttcagcaat ggcgctgttc
     4681 agagtgccat cacgttgtat ggatatccgc acggtgcaca tttttcccgc ataggcctct
     4741 ggttcaaaca tctgcatttc aacagcggtc ttgatggccg cagcgtagct actgatttta
     4801 gatgacttat ccagagtata aattttcggc ggctgggtgg tggaatgctt acaggccgtc
     4861 aggagtaagg ggctgcacaa tgccagcagg ataaagggta ttaaacgcat cgggttgatg
     4921 acctgtcagt aaaaaatgtt acatgtatat ttgtcactct ttacggccac gttcatttac
     4981 ataaacacgg cttatcgtat cgtttttaca ccaaacggcc cgccatgcag ttgcgtgcat
     5041 aggcatgcag cgggttacaa tttaccgggc gcgtttccgg cggttttccg ggtggtttgt
     5101 tgcctgtttt atcccgtagc cgccggaaac gccctcagac cgtctgagcg gtgcgggcaa
     5161 tgcgacgtta tggtaaatac cccttaccgg cggcgctgtg gctgccaggt gaaggtggtt
     5221 tccgtagcct cgtatttctc ccgacgcatt tcctgcgccc gcctggcttc acgttcgtcc
     5281 tgaatggcac gaagcaccct ttcccgcacc tgttgtttta ccgcctttgg cagcgttttg
     5341 ttccctttca ggcccatggc cagtgcctga gcgacaaagg cgtccgccag gcgcttttct
     5401 tcagcgggag tcagtttcag gtgcgtcata gtggcctccg ggcgtctcag gtttcccgaa
     5461 cagtttaacc gcaggtcctc tcctcatgcc agttagcttt cagatgtgtc tataccatct
     5521 gaaatgtaag tgtgatgaat gtcttaaata ataaaatttg ttgccatgag aaattttcgg
     5581 gaaaagacgc gatctgctta caggtggctc cgggcgcttt tttgtgcctg aagatgagaa
     5641 ctaatctcat atattgacac ctgaaaacgg gaggagtaag gtaataatta tactgtgtat
     5701 atgtacagtg tgcgccggga gaccggttag agatcaaggg gtgaaagtcc ccgaccattg
     5761 aaggaccagc aatccacaag gtccccgagt catgcgttgc ataccgcgag gtatggggcg
     5821 aagcgttgac aggggtgttg acaggccagc cattgagcca cgaaatgtat attaaattac
     5881 cgggtgccga cgttgtactg ttaacggaag gcaacatcat agggtgcgac actgcgagtg
     5941 ccacatggac ccggcggggt ctgagaccct ggcatgtcaa tacgatctct acgcgggaac
     6001 cgggagatct cccctctgac catctgccag tgtcggagat ggcccgcacc gggaagacga
     6061 ggagtcatag ccggtgatgt acggagagga gaagtcggac tcgctcatag tagcggcgaa
     6121 gcaggcgaac aacccgaaag gagcggagtc agtggagcga aggagcgggg ccaaggggaa
     6181 cgcggaacag ccacacatgc gccggacaca gagccgggaa agcatgtcac agaggctgtc
     6241 acgcgtgcgg gaagctgcga agcagcggaa gaaagaacgg tttacagcat tgttccacct
     6301 gctgacagtc gaagcactgg aagccgcatt cctctccctg agcaggaaag cggccgccgg
     6361 agtggatggc atcaggtgga tggactacgc cggaaacatg aagaacaaca taacagatct
     6421 gcaccggagg ctacatcagg gcagctacag ggcgcagccc ggcaggcgtc actacatccc
     6481 aaaagcggat ggaaaacaac gcccgctcgg catcgcctcg ctggaggaca agatcgtcca
     6541 gtatgcgctg gtgaaaatcc tgaacgcagt ctatgaaaac gactttatgg ggttctcata
     6601 cgggttcaga cccgggcgaa gccagcacga tgcactggac gcactggcca cagggctggt
     6661 acgcactaac gtaaactggg tactggatgc cgacatcagt cagttcttcg acagggtgag
     6721 ccacgaatgg ctgatcaggt tcacagagca tcggatcggc gaccggaggg taatcaggct
     6781 catacgtaag tggctcacag ccgggacgtc ggaggagggt caatggcgag caacggagga
     6841 aggcacccca cagggtgcgg tcatctcacc gctgctggca aacatatacc tccactacgt
     6901 cttcgatctg tgggcgcatc agtggcgacg tcgctatgcc acaggcaatg tggtaatggt
     6961 cagatacgcc gatgacatcg tcatcgggtt cgacaaacga tacgatgccc ggcgcttccg
     7021 tatagccatg cagcgcagac tgagggagtt cggactcacg gttcacccgg agaaaacccg
     7081 tctgatggag ttcggccgct tcgctgccga aaaccgtgcc atcaggggaa aaggcaaacc
     7141 agaaacgttc aacttcctcg ggttcacgca catcagcggg aaagatcgca acggcaggtt
     7201 catgctgata cgaaagaccc gccgggatcg gatgacggca actctgaaag ccatcaaaga
     7261 cggtctgcga aggcgctggc attactcaat ccccgaacag ggaaaatggc tcaggagagt
     7321 ggttcaggga tacctgaact atcactcggt accgggcaac ttccccacca tgcagaagtt
     7381 caggacacac gtaacaaacc tctggcgccg ggcgctcagg cgcaggagcc agaaggatga
     7441 tacgacctgg acgaaagcaa acaaactggc agccgcatgg ctaccaaggg ttcgggttct
     7501 tcatccatgg cctgtggagc ggttcaccgc cagacacccg aggcaggagc ccggtgcgta
     7561 aatcgcgcac gccgggatct gtgcgggggg tatccggtaa cgggtatccc taccgcgaca
     7621 tgttggcttt aatttattgt tttaaatgtc aaagaggaaa acggtatgag tggtggagac
     7681 ggtcgaggtc cgggtaattc aggtctggga cataatggtg gtcaggccag tgggaatgtg
     7741 aacggtacgt ctggtaaagg tggcccttca tcaggtggtg gtacggatcc aaacagcggg
     7801 ccgggctggg gtacgacgca tacgccgaat ggagatattc ataactataa tccgggggag
     7861 tttggtaacg gcgggagtaa acccggtggt aatggtggta acagcggcaa tcatagcggt
     7921 agctctggtg gcggacagtc ttcggccact gcgatggcct tcggtctgcc tgccctggct
     7981 actccggggg ctgaaggact ggctttatca gtttccggcg atgcgttgtc ggccgccgtt
     8041 gctgatgtgc tggctgccct gaaggggccg tttaagtttg gtctgtgggg gattgcgatc
     8101 tacggtgtgc tgccttctga gattgcaaaa gatgatccga aaatgatgtc aaaaattatg
     8161 acgtcattac cggccgatgc ggtgacggag actccggcaa gtactttacc actggaccag
     8221 gcgacggttc gtgtcagaca acgggttgtg gatgtggtga aggatgagcg gcagcatatt
     8281 gcggttgtcg caggtcggcc aatgagtgtc cctgtggtgg atgcgaaacc gacaaaacgt
     8341 ccgggggtat tcagtgtgtc gattccgggt ctcccgtctc tgcaggtgag cgtaccgaaa
     8401 ggtgttccga cagcgaaagc cccgccaaaa ggcattgttg ctgaaaaagg tgattcacgt
     8461 ccggctggtt ttacagccgg tggtaactcc cgtgaggccg ttattcgttt cccgaaagag
     8521 accggacaga agccggttta tgtgtcggtg acagatgttc ttaccccggc acaggtaaaa
     8581 cagcgtcagg aggaagaaaa gcgtcgccag caggcatggg acgccgctca tccggaagag
     8641 gggctgaaaa gagactatga taaagcgaaa gctgagctgg atgccgaaga taaaaatatt
     8701 gcgaccttaa acagccgcat tgcatcgaca gagaaggcgc tccccggtgc aagggctgct
     8761 gttcaggaag ccgataaaaa ggtaaaagag gcagaggcaa ataaagatga ttttgtgact
     8821 tataacccgc ctcatgaata tggctccggg tggcaggatc aggttcgcta tcttgataag
     8881 gatattcaga atcagaatga gaaattaaaa gcggctcagg catctttaaa cgcaatgaat
     8941 gaatccttat ccagagataa ggctgcactt accggggcga tggagagccg gaaacaaaag
     9001 gagaaaaaag cgaaggatgc agaaaataag ttaaatgagg aaaagaacaa gcctcgcaag
     9061 gggactaaag actacggcca tgattatcat ccagtaccta aaacagaaga cataaaggga
     9121 cttggagagt taaagcgagg tgatcctaaa acacctaaac aaggtggtgg tggtaagcgg
     9181 gcccgatggt atggcgataa aaaacgtaaa atttatgaat gggattccca gcacggtgag
     9241 cttgaagggt atcgtgccag tgatggcgaa cacctaggcg catttgatcc caaaacgggt
     9301 aagcaggtta aagggccaga tccaaaacgg aacattaaaa aatatctttg agaggtaaat
     9361 atggggctta aattacgttt gacatggttt gataagaaaa ctgaagagtt taaaggggaa
     9421 gagtattcta aagactttgg tgacgatggt tctgtcattg aaagcttggg aatgccttta
     9481 aaggataata ttaataatgg tgagtttgat ttgaagaaag aatgggttcc tttactacag
     9541 ccatttttta aaaatcaaat aaaacctgat gtggataatt gttatatagc ttttgattat
     9601 cgggacggta actggtaaca aaaaattatc aaggatgatt tgaaatactg acttccggtg
     9661 ttcagggatg aatgccggag tctttaaata cagggaaagt atatgaaaaa ggtaaagact
     9721 atttttcttt ttatattgat tgttgctggc tttcttctgg ccgcatgtca ggcaaattat
     9781 atccgggatg ttcagggcgg aacggtggca ccatcgtcct cctctgaact gacggggatc
     9841 gcggttcagt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     9901 tttgccctgt aaacgaaaaa accacctggg gaggtggttt gatcgaaggt taagtcagtt
     9961 ggggaactgc ttaaccgtgg taactggctt tcgcagagca cagcaaccaa atctgtcctt
    10021 ccagtgtagc cggactttgg cgcacacttc aagagcaacc gcgtgtttag ctaaacaaat
    10081 cctctgcgaa ctcccagtta ccaatggctg ctgccagtgg cgttttgcgt gctttcccgg
    10141 gttggactca agtgaacagt taccggataa ggcgcagcag tcgggctgaa cggggagttc
    10201 ttgcttacag cccagcttgg agcgaacgac ctacaccgag ccgagatacc agtgtgtgag
    10261 ctatgagaaa gcgccacact tcccgcaagg gagaacggcg gacaggtatc cggtaaacgg
    10321 cagggtcgga acaggagagc gcaagaggga gcgacctacc ggaaacggtg gggatcttta
    10381 agtcctgtcg ggtttcgccc gtactgtcag attcatggtt gagcctcacg gctcccacag
    10441 atgcaccgaa aaagcgtctg tttatgtgaa ctctggcagg agggcggagc ctatggaaaa
    10501 acgccaccgg cgcggccctg ccgttttgcc tcacatgtta gtcccctgct tatccacgga
    10561 atctgtgggt aactttgtat gtgtccgcag cgcccgccgc agtctcacgc ccggagcgta
    10621 gcgaccgagt gagcgaggag gcgcaatttc cccggcacct gcaagacggt caccggacgc
    10681 gctgatgtgg gatattgtcc cctgattaag tctttctatt ccctgacgat caattatcag
    10741 gataggtgaa ccatgtcggt ttttcgctga acattttacg ttaacctatc tctgttgctt
    10801 ttttctcctt gcggagaaat gcaagtcatc cccgctgggg cgtggctgac ttcaggggaa
    10861 aagaccttgc ttacgctggc tggacgccac cgacgcacgg tcttttcccc tctttctggc
    10921 tctgtccggc tggacgccgg ccacgccaga aattcacccg gcccccgtca ggctcacgcc
    10981 tgaccaccca caaaaatcca cggtgctgga cgcacctgac gaagcagcaa agctgctcct
    11041 gattttgcgg gtggtcagcc gctcgctatg gcgaactctg acgggggccg gaacaccgga
    11101 aaagaccgca ctggggtgcc gccttttcct ctcacacgct ggggcgtgct cgttgttcct
    11161 ccggggctgc gccttttccg catggggcgg aaagaggctg gcctccgcgg tgaaagctga
    11221 acgcaatcac cacggacgtg accgccgccg ccatgccttg gggcatgtcc gcggcttgtg
    11281 cagcgggggc tgcgcatcac gccggtaaac ccgtcgtctg aaggagagag attgcatgtt
    11341 taatacggat tcgcttgcct ggcagtggca tatcccggag ctggcccggc agatttaccc
    11401 gtgggtcagc tggctgtacc gggagtatta cagtctggcc ggtttttttc tggttatcgg
    11461 cctgtttttc ggtctggctg aactctgggc cggccgaatg cgttttgtgg ggctgagcca
    11521 taaggcgcgg cgggagtatg ccgatcgcta taagcgcgat aataaccgga ctgtgccatt
    11581 ttcggtgctg gcctcgctgg attcacccgg gcggatggtc agggcacggc gacatgtctg
    11641 gaactacgcg tactggctga ccggcgctgc cggtctggcc cttctggggg ggctggttgt
    11701 cgccgtgttt atcgagagct ggggagaaac cgtcctgcgc ccggacctct atgcgaacgc
    11761 agattattac ctgctgcagc cggctaacct gttcgggata gcctggtgtg ccggagggct
    11821 tgtcgccggc gtccttctgg cgcggctgct ggcgttcctg ctgcttgatg gtcattttgc
    11881 ggccgctgac gaggcggtga acgcgaaact gaatcaggag agccggcgca gcagccagcg
    11941 gaccggagaa atgacagacg tccgccacct
//
