LOCUS       NZ_CP054386             1822 bp    DNA     circular CON 02-JUL-2023
DEFINITION  Escherichia coli strain SCU-175 plasmid pSCU-175-7, complete
            sequence.
ACCESSION   NZ_CP054386
VERSION     NZ_CP054386.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14943546
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Stephens,C., Arismendi,T., Wright,M., Hartman,A., Gonzalez,A.,
            Gill,M., Pandori,M. and Hess,D.
  TITLE     Title: F plasmids are the major carriers of antibiotic resistance
            genes in human-associated commensal E. coli
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Stephens,C., Arismendi,T., Wright,M., Hartman,A., Gonzalez,A.,
            Gill,M., Pandori,M. and Hess,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) Biology, Santa Clara University, 500 El
            Camino Real, Santa Clara, CA 95054, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method              :: Unicycler v. 0.4.8
            Genome Representation        :: Full
            Expected Final Version       :: Yes
            Genome Coverage              :: 81x
            Sequencing Technology :: Illumina MiSeq, Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 07/02/2023 04:33:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 2
            CDSs (total)                 :: 2
            Genes (coding)               :: 2
            CDSs (with protein)          :: 2
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP054386.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="SCU-175"
                     /isolation_source="rectal swab from healthy college
                     student"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pSCU-175-7"
                     /geo_loc_name="USA: California"
                     /collection_date="2017"
     gene            complement(672..959)
                     /locus_tag="HPE51_RS00005"
                     /old_locus_tag="HPE51_27730"
     CDS             complement(672..959)
                     /locus_tag="HPE51_RS00005"
                     /old_locus_tag="HPE51_27730"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001220925.1"
                     /translation="MRIKYESRWKLEELKLEICRSIDSLIDEYHIEYAMGINLYMNCED
                     KEFNPVDIVNEDGKKIMFVVSNNEKIQKPKKSAKKKIPAPVIELDKYRKK"
                     /name="hypothetical protein"
     gene            complement(972..1505)
                     /locus_tag="HPE51_RS00010"
                     /old_locus_tag="HPE51_27735"
     CDS             complement(972..1505)
                     /locus_tag="HPE51_RS00010"
                     /old_locus_tag="HPE51_27735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005707163.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_000893324.1"
                     /translation="MLEQQFEDFEQQKRRDHQAQDQSKSAKQKRDRLNPPDWSQVTKAG
                     WNMLDSISEKVPRAFRLYSFFAQNIDKSCGAVVCDQKFLADYFGVSLRTIQYWIGDLES
                     IGAIVKIPVGIYYAYALDPYQVWRGYNKSKKYAAFNAKTLTQKDGVIDRKLRLMLSRRE
                     EFEGSLKTSNHDLD"
                     /name="replication/maintenance protein RepL"
     Typing          170..430
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.366"
                     /coverage="100.0"
                     /evalue="3.98e-101"
ORIGIN
        1 atttcgcgct ctccacgttc aacagctcga tcatgttttt gcagtccgtt caccgctaaa
       61 acgccccgca tcagtgcgta cgtgctcaat gtggtttccg gttctcttcc gcttcctcgc
      121 ccactcgctc actaacgttc ggtcgctggg gctgcggcga gcggtgccac ctctcgcatg
      181 ttatcggtga ttccgctgtt gttctttcgg ggatgagtcg ccagatagct cttcaaacgc
      241 tctcagaagc tctcagacgc acgaaacggg ttttaaatgc ccatcagggc atggaaggcg
      301 cgaaaaacgc ttacagaggg ttttaggagg tcaggcgcgc cgatacccct aaccaggggg
      361 tttcaggtcg ccaggaaccg cgtcagcccc ctgcaaagcc catttgcacg gaataggcag
      421 ccatgcggtt ggtaggtggt cgtagaccgt cgatgttaca gcgacggggg tctggggttc
      481 tccccagacg gtttccggtt gcgctcaagg cgctgccgga accgtggcgg ctcggtcgtt
      541 ctggaaaacg gggagcaaaa agacgcaagg gattttgagg cggcaaaaga gcaatgcagg
      601 atagacaatc aactagaaat tgcatgcaat gttctgtgga ttgtccgcct accgctggct
      661 ctgtatggcc atcacttttt tcgatattta tctaactcaa ttacaggtgc aggaattttt
      721 ttcttcgcac ttttttttgg tttttgtatt ttttcattgt tgctaacgac aaacataatt
      781 ttctttccat cttcattgac aatatcaacg ggattaaact ccttatcttc acaattcata
      841 tatagattta ttcccattgc atattcgata tgatattcat caattaacga atctattgat
      901 ctacatattt ctaattttag ttcttctaat ttccaacgtg attcatattt aattctcata
      961 gcgcatcata cctagtccaa gtcatgatta gatgtcttaa gcgagccttc aaactcttct
     1021 ctcctagaga gcatgagccg caacttgcga tcaataacgc catctttttg agttaatgtt
     1081 tttgcgttaa acgctgcata ttttttagat ttattatatc cgcgccatac ctgatacgga
     1141 tctaacgcat aagcataata gatacctact ggtattttca cgatggcacc aatactttct
     1201 aaatctccaa tccaatattg gatcgtcctt aaactaacac cgaaataatc agctaagaat
     1261 ttttggtcac atacaacagc cccacacgat ttatctatat tttgagcaaa aaaagaatat
     1321 agcctaaatg ctctgggaac cttctcagat atagagtcca acatattcca gcccgcctta
     1381 gtaacctgcg accagtcagg tggattcaac cgatctcgtt tttgcttagc acttttggat
     1441 tgatcttgtg cctgatgatc tcgccgtttt tgctgttcga aatcttcaaa ttgctgttcc
     1501 agcatacttg catccttacc tgcaacgttg tttcgtacct tacgaaattt atatcgtaca
     1561 ttacgaaaca acacacgcaa acatgtttgc gtatacacat gcaaataacg tttcgtatgt
     1621 tggtgtcata atgctttgat ttttaagaga atgtcgattt ttgctctaaa gatctatcaa
     1681 agcacttttt tgctacacaa gatttaatta gttcatcgaa catttggggt gtcggggcga
     1741 agccctgacc cagtcacgta gcgatagcgg agtgtatact ggcttactct tctgcaagcc
     1801 tcgtcagccg tgattttgcc cg
//
