LOCUS       NZ_CP054298             8828 bp    DNA     circular CON 12-MAY-2024
DEFINITION  Citrobacter freundii strain IDR1800045912-01-00 plasmid p1C9,
            complete sequence.
ACCESSION   NZ_CP054298
VERSION     NZ_CP054298.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15086845
            Assembly: GCF_014170055.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter freundii
  ORGANISM  Citrobacter freundii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1  (bases 1 to 8828)
  AUTHORS   Prussing,C., Snavely,E., Singh,N., Lapierre,P.,
            Lasek-Nesselquist,E., Mitchell,K., Haas,W., Owsiak,R., Nazarian,E.
            and Musser,K.
  TITLE     Nanopore MinION sequencing reveals possible transfer of blaKPC-2
            plasmids across bacterial species in two healthcare facilities
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8828)
  AUTHORS   Prussing,C., Snavely,E., Singh,N., Lapierre,P.,
            Lasek-Nesselquist,E., Mitchell,K., Haas,W., Owsiak,R., Nazarian,E.
            and Musser,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUN-2020) Division of Infectious Diseases, Wadsworth
            Center, New York State Department of Health, 120 New Scotland Ave,
            Albany, NY 12208, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 02-OCT-2019
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 183x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014170055.1-RS_2024_05_12
            Annotation Date                   :: 05/12/2024 03:50:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,127
            CDSs (total)                      :: 5,006
            Genes (coding)                    :: 4,896
            CDSs (with protein)               :: 4,896
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 110
            CDSs (without protein)            :: 110
            Pseudo Genes (ambiguous residues) :: 0 of 110
            Pseudo Genes (frameshifted)       :: 27 of 110
            Pseudo Genes (incomplete)         :: 75 of 110
            Pseudo Genes (internal stop)      :: 21 of 110
            Pseudo Genes (multiple problems)  :: 13 of 110
            Pseudo Genes (short protein)      :: 1 of 110
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP054298.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8828
                     /organism="Citrobacter freundii"
                     /mol_type="genomic DNA"
                     /strain="IDR1800045912-01-00"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:546"
                     /plasmid="p1C9"
                     /geo_loc_name="USA:Maine"
                     /lat_lon="45 N 69 W"
                     /collection_date="2018-10"
                     /collected_by="Maine Center for Disease Control and
                     Prevention"
     gene            complement(join(8556..8828,1..30))
                     /locus_tag="HS089_RS25445"
                     /old_locus_tag="HS089_25450"
     CDS             complement(join(8556..8828,1..30))
                     /locus_tag="HS089_RS25445"
                     /old_locus_tag="HS089_25450"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045889494.1"
                     /translation="MAKSRAEKLEELRQKEAQIKAQIQQLKQREQTEERKKDTRRKILI
                     GGAVLAKIKRGEWNYQQLVSLLDNELKNDRDRALFDEMGLKAVDARKESFNPGNK"
                     /name="hypothetical protein"
     gene            190..2013
                     /gene="mobQ"
                     /locus_tag="HS089_RS25805"
     CDS             190..2013
                     /gene="mobQ"
                     /locus_tag="HS089_RS25805"
                     /inference="COORDINATES: protein motif:HMM:NF015354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_072052381.1"
                     /translation="MAIFHLSVKTISRSAGRSATAAIAYRTGSRIVDERTGIIHDYTRK
                     SGVEHSEIFLPQNSPDWASNRETLWNAVEQKENRRNSTVAREFELAFPAELDGKQRLEL
                     LRDFCSSLVERHGMAVDAAIHAPGKEGDQRNHHAHVLCSTRRLSEEGFKDKTRELDDFK
                     SGEVNHWRSAWSEMTNRHLEKAGRSERIDHRSLEAQRESAISQGDSAKAAKLDRVPTIH
                     IGYAAAQMEKRGLETERGNEYRQIILRNAEIQPLSRAIQLIDNWRATHDNNSRAGHGSI
                     APVPERDKGRDKRGVRTAQSYTGRVGAVPPPSARGRLRNLSELDMVRFGERSEMLLPGN
                     VSRDVEHQRTDRNQPLRRENTVTSKSKRPEVIRLEWQSLRRDKLTFVQDKAGRIAARGA
                     AHVERQRQKISSHERQRPEQPSGLIGMFKRSAYEQAASTWQTIKNGLDRRLVQLKNRLG
                     FVRDYMRKSGAYEMSTPGERLADQLAAKENPILAGQIKSLNAQKNAEDMAAYRTRVQSR
                     KQEKADMKLETIEELKQRGDARRDKADKAERAELEKASHQASLQNETLSEETKELESGE
                     KEKLSKRDQVLEKFKAKVERDKQNERGGRGR"
                     /name="mobQ"
     gene            2156..2575
                     /locus_tag="HS089_RS25455"
                     /old_locus_tag="HS089_25460"
     CDS             2156..2575
                     /locus_tag="HS089_RS25455"
                     /old_locus_tag="HS089_25460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063058.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_182945395.1"
                     /translation="MSEEQRRRGFMKAEASLRLEGIDPSGSPRYESVKARIISGEITYD
                     QGRAEILAFHTGKRAEPTTAGEMLAEEFLKPLNMSQGELAQATGIPEHHIDDIISNRRR
                     LSAEESYKLAEQFQTDKDFWLNLQALHDAWLTHQK"
                     /name="HigA family addiction module antitoxin"
     gene            complement(2736..3089)
                     /locus_tag="HS089_RS25810"
     CDS             complement(2736..3089)
                     /locus_tag="HS089_RS25810"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_227664254.1"
                     /translation="MITQLKGRKIALVSFCWYELICTQMNGNQFSQEKKVKGSKPSIWS
                     ITEGEVIMATINLSLLEHDCGEAAREALLSGVPVYYADETTPENCVIKEYPDGRRELVS
                     FMTGMEKLVGANT"
                     /name="hypothetical protein"
     gene            3506..4024
                     /locus_tag="HS089_RS25465"
                     /old_locus_tag="HS089_25470"
     CDS             3506..4024
                     /locus_tag="HS089_RS25465"
                     /old_locus_tag="HS089_25470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045889486.1"
                     /translation="MSVRAQRAGKRNQSERRIELINTLWEGEEIETWDRNDRPRNDGFI
                     TVPRYLPLLGVLMDELSKGSPLSSTYLALWFRVSDEGLIEIRDKTVLALESGFASGRGV
                     TTWTGRMRKLKELGFISCREGSSGEFHNVLIVHPLVAVKKLLDEGKITKGKTYNTFAER
                     VIEVKSSWE"
                     /name="hypothetical protein"
     gene            4028..4522
                     /locus_tag="HS089_RS25470"
                     /old_locus_tag="HS089_25475"
     CDS             4028..4522
                     /locus_tag="HS089_RS25470"
                     /old_locus_tag="HS089_25475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_182945396.1"
                     /translation="MAGTVWNDVNTGREVFSMTHLHPQLHKVEVSGVQAEIEISFGFHV
                     FTDQKQVGKVMSFRGETRHFCQERYEGSKTIVQRILSAIENGEYITAFISKGQGQRYYH
                     LNHHDDFILMEIRKPEGKDNFLRIHVVTAYTLDEWGTVNKGRNLRFRYVLEQRLQGKKI
                     I"
                     /name="hypothetical protein"
     gene            complement(4910..5050)
                     /locus_tag="HS089_RS25475"
                     /old_locus_tag="HS089_25480"
     CDS             complement(4910..5050)
                     /locus_tag="HS089_RS25475"
                     /old_locus_tag="HS089_25480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_062939423.1"
                     /translation="MQEPPVQPDCCALPGNYRLESNPGRHDKAPLAAAIGNWEFAENLF
                     S"
                     /name="hypothetical protein"
     gene            5410..5772
                     /locus_tag="HS089_RS25480"
                     /old_locus_tag="HS089_25485"
     CDS             5410..5772
                     /locus_tag="HS089_RS25480"
                     /old_locus_tag="HS089_25485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019105480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045889489.1"
                     /translation="MAWHSVSQAQNMTGKSRRTLYRDMASGRLSWRTNTSGHRELETSE
                     LIRVYGEIKNDGTAERHTVSQPDVTVSGSDIRDELRALRQEVADLKSMLLRLEHKQEEQ
                     PETAAPWWRFWQKKST"
                     /name="hypothetical protein"
     gene            complement(6025..8508)
                     /locus_tag="HS089_RS25485"
                     /old_locus_tag="HS089_25490"
     CDS             complement(6025..8508)
                     /locus_tag="HS089_RS25485"
                     /old_locus_tag="HS089_25490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934115.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA transfer
                     family protein"
                     /protein_id="WP_220384729.1"
                     /translation="MKKNILAGIFFVAMLLPVWHAAAETFTRCHPNETVWAMIQDEQLK
                     NIARLQETKRCQAVNDTYDQWRQNFQAMGDAENAIRSARDHQNWNAVKSTFDNIAPQIN
                     SLHALAEKNSSLLGAGNILALFGGDLNYLYQNAGLVAPASADGLTDNVSQMLTEPDKQV
                     KAAGIAQQLVYQTRVRAQSFIQGVAQNAGNEAQKEYRAQSDKIVEDKAHNFKGDSVEGY
                     FGGFLARISQVYAPVLLFLIIVGIIGALTARRVRLDAFTASVKAVMAAAVPAVALALML
                     IFMPFLPSWVLYAAALAVTFAIYSRSKKILGGLASRCGRFPALASGLRWMGGFVSGLDA
                     DRKQETGAASVNPASHHTHGSSRWGTAAEMRENNHLLPTEKQAGFTLARELSPPPGVDG
                     KFCFTGHVVTVAPTGSGKGIGAVIPNLLTYQGSALVFDPKGENAEVTGRRRREMGQSVH
                     VIDPFGITSGIKESVNILDRLDVTSPDCVAESAVLAESLVVSEKGGDGNHFDESARTLL
                     QGLMLHVSGLPDESRRNLAELRRLITSDEENLLALLADMAASEDVAFGIPARAANTLMG
                     MADRERGSVLSTARKNTAFLDDPRIAAALSHSDFNLSAIKAEPMTVYLVLPANRIAANT
                     RFVRLFIGSVISAVTESKAKPAHNVAFILDEFNVLGYMKSIEDAVSLLRGYGLSFWVFL
                     QDLSQLKSAYPKWQTFLANSAKTFYGTDDYDTAKYVSDTLGKSTVEFETQNSGRNSGGG
                     VSGGGGSMNRGRSSGSSQQFAGRELLTPDEVMRLGPTRPIVILKGEYPYQLTRINYLTD
                     PEYTGMADPNPYYSA"
                     /name="type IV secretory system conjugative DNA transfer
                     family protein"
     Typing          complement(4942..5309)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.566"
                     /coverage="85.0"
                     /evalue="8.49e-135"
ORIGIN
        1 ttccaatttt tccgctcttg attttgccat tagtcacctc gttgattcaa atttgaacga
       61 taacccctgt actgatatat ttcaacccga agcgaaagca aagggcgcac ttatgcaaac
      121 tcttcgagtt tgcgtgcggc agggttaaaa ccctgcaccc aaggtcaacg gaaaaacagg
      181 aatacgaaat tggcaatttt tcatcttagc gttaagacaa tcagccgtag tgctggccgt
      241 tcggccactg cggcaattgc ttatcgtaca ggttcaagaa ttgttgatga acggactggg
      301 attatccacg actacacccg taaatcaggc gtcgagcatt cagagatttt tctccctcaa
      361 aactcaccag attgggcgag taatcgggag actctatgga atgcagttga acagaaagaa
      421 aaccgtcgta actcaaccgt tgccagagaa tttgaactcg catttcctgc agagcttgac
      481 ggaaaacagc gtctcgagct gcttcgtgat ttttgttctt cgctggttga gcgtcacgga
      541 atggcagtgg atgccgctat ccatgctccg ggtaaagagg gagaccaacg taatcatcat
      601 gctcacgttt tatgcagcac ccgccgactg tcggaggaag gtttcaagga taagacccgt
      661 gaacttgatg attttaagag cggcgaagtc aaccactggc gttctgcatg gtcagaaatg
      721 acgaaccgtc atctggaaaa ggctggccgt tcagagcgta ttgaccatcg ttcacttgag
      781 gcgcaacggg aatcagctat ttctcagggg gattcagcca aggcagcaaa gctggatcgg
      841 gttccgacta ttcatatcgg ttacgcagct gcgcagatgg aaaagcgcgg cttagagacc
      901 gaacgaggca atgaatatcg ccagattata ttgcgtaatg ctgaaatcca gccgctttca
      961 cgggcaattc agctgattga taactggagg gcaacccatg acaacaacag cagagcagga
     1021 catggaagca ttgctccagt ccctgaacga gacaaagggc gagataagcg aggagtccgc
     1081 accgctcagt cctacactgg ccgcgttgga gccgtcccgc cgcccagcgc aaggggtcgc
     1141 ctgcgcaacc tgtccgaact cgatatggtt cgcttcggag aacgaagtga gatgttattg
     1201 ccgggcaatg tatctcgtga cgtggagcac cagagaaccg acagaaatca gcctctgcga
     1261 cgggaaaaca ctgtaaccag caagtcaaaa cggccggaag ttatccggct tgaatggcag
     1321 agtttacgcc gggataagct gactttcgtt caggataagg ccggacggat tgccgcccgt
     1381 ggggcggcac atgttgaacg ccagagacaa aaaatatcct cacatgagcg acaaagacca
     1441 gaacagccca gcggcctgat aggaatgttt aaacgcagcg cctatgaaca ggccgccagt
     1501 acctggcaga ccatcaaaaa tggcctcgat cgtcgtctgg tgcagctgaa aaatcgactt
     1561 ggctttgtca gagactacat gcgtaagtct ggcgcttatg aaatgtctac gccgggagaa
     1621 cggctggccg accagctggc cgcgaaagaa aatccgatct tggctgggca gataaaatcg
     1681 ctcaatgctc agaagaacgc ggaagatatg gctgcatacc gtacccgcgt acagtcccgc
     1741 aaacaggaga aagctgatat gaagctggaa actatcgaag aactcaaaca acgtggtgat
     1801 gcccggagag ataaagccga caaagccgaa cgtgcagagc tggaaaaagc gtctcatcag
     1861 gcttcactgc agaatgaaac gctcagcgaa gagacaaaag aactggagtc cggggagaaa
     1921 gagaaactca gcaagcgtga tcaggttctg gagaagttta aggcaaaagt cgagagggat
     1981 aagcagaatg aacgtggtgg ccgtgggcgt tagcggtatg caacacagcg ccaaccatgc
     2041 tgcatacctg acccggccag actgatcagg cgtcttttcg ggctgaatgc attgtactgg
     2101 ttaacattat ttcatgacat atgaggtacg cctgatattg acatatgagg tagatatgag
     2161 tgaagaacag cgccggcgtg gcttcatgaa ggctgaggca agcctcagac tggaaggtat
     2221 agatcccagt ggaagccccc gctacgagtc ggttaaagcg agaattattt cgggtgagat
     2281 aacctatgat cagggacgag ctgaaattct ggctttccat accgggaagc gtgcggagcc
     2341 gacaactgcg ggggaaatgc tcgcagagga atttttaaaa cctctgaaca tgtctcaggg
     2401 tgaactggcg caggctacag gaattcctga gcatcatatc gatgatatta tcagtaaccg
     2461 ccgtcgcctc agtgctgagg agtcttacaa gctggccgaa cagttccaga ccgataaaga
     2521 tttctggctc aacctgcagg ctttgcatga cgcatggctg acgcaccaga aatagcagcc
     2581 aggggcgcga ttagatatat aatgttccag tctaatgtag gatgttccat gcaggtcaga
     2641 gtattaaaaa taaagccccg tccttaccag gcggggcttt attttttcct gcccctcaag
     2701 tgtcataatg gttctgaaat ccggacatcc aaaaatcatg tattagcccc aacgagtttc
     2761 tccataccag tcatgaagct caccagttcc ctgcggccat ccggatattc tttgataacg
     2821 cagttttctg gtgtcgtctc atcggcgtaa tagacaggaa cgccgctgag aagagcttcg
     2881 cgggcggctt cgccgcagtc atgttccagt agtgatagat taatggtcgc catgatgact
     2941 tctccttcgg taatactcca aatggatggt tttgaccctt tgaccttctt ttcttggcta
     3001 aattgattgc cattcatttg tgtgcatatc aattcatacc aacaaaaact caccagtgcg
     3061 attttacggc cttttaattg ggtaatcatg gtcttttgag gcttagggca taaaaacgcg
     3121 ctgagggcct ctgaggcggt tctgctaacg gatgttatgg taaatacgag tgaccggcat
     3181 ggtgactctt aatttatttt tcgatttctc cacccctcct tgccccctac aaccccatgg
     3241 agcacaaggg agcaccggaa gcacatggag cacggtgagc acatggagca ccgcaattaa
     3301 ataaaaactc tttttaaatc atgttaatgc gaattctttt tgtaatatat attacttata
     3361 actgccgttt atgtaataaa tattgcattt tttatggtta aaaaaaataa aaactggaag
     3421 taattgtccg agggaattta taaaagttat tgatttataa ggattttttg gtaaattggc
     3481 tctcatgcat taacaggagg cgagtatgtc agtcagggca caaagagcag ggaaacgtaa
     3541 ccagtctgaa agaagaatcg aactgataaa tacattatgg gaaggggagg aaattgaaac
     3601 atgggatcgt aatgaccgac ctcgaaacga tggcttcata acagtacctc gttatcttcc
     3661 attactaggt gttcttatgg atgagttatc aaagggaagc ccactttcaa gtacctacct
     3721 agcgttgtgg ttcagggttt cagacgaagg cctgattgag ataagggata agactgtgct
     3781 ggctttagaa tcaggctttg ccagtggaag gggggtaaca acatggacgg ggagaatgcg
     3841 gaaattaaaa gagctgggat tcatatcatg ccgggaaggt agctccggtg agttccacaa
     3901 cgtgttgatt gttcatccgc tggttgcggt aaagaaacta ctggatgaag ggaaaataac
     3961 gaaagggaaa acctataaca cttttgctga acgggtgatc gaggtgaagt cctcatggga
     4021 gtaagaaatg gcaggtacgg tatggaatga tgttaataca gggcgtgaag ttttctcgat
     4081 gacacacctg caccctcagc tgcataaggt tgaggtttct ggggtgcagg ctgagattga
     4141 gattagcttc gggtttcatg tatttactga ccaaaagcaa gtcggaaagg taatgagttt
     4201 cagaggtgaa actcggcatt tctgccagga aagatacgaa ggaagtaaga ctatcgtgca
     4261 gcggatactg tcagcgatag aaaacggaga gtacataacg gcctttattt ccaaggggca
     4321 agggcagcgg tattaccacc ttaaccatca tgatgacttc attctaatgg agataagaaa
     4381 gcctgaaggt aaagataatt ttcttcgcat acatgttgtt acggcatata cgctggatga
     4441 atgggggacg gtcaacaaag gacggaattt gaggttcagg tatgttttgg agcagaggtt
     4501 acaggggaag aaaatcatat aaaaaacaaa aagcgcccaa ggcgcctttg ttttactgca
     4561 aaaatttcgg tctggccttt cgttatcccg ctcactggct aggagggttg cccccggcat
     4621 cagcacccct ctttcaaggt tccggtttgt gctgacaggt atattgtcca cttaccaatg
     4681 ccacttgtca acttcacggg tttgtcatga tcttcttgag atcctttgtt tatcgtcgta
     4741 gtctcttgtc cagtaaacga aaaaaccacc cggcaaggtg gtttttcgaa ggttcagtaa
     4801 accggcaagt ttctgaaccg tggtaactgg ctttcgcaga gcacagcaac caaatctgtc
     4861 cttccagtgt agccgtactt tggcgcacac ttcaagagca accgcgtgtt tagctaaaca
     4921 aattctctgc gaactcccag ttaccaatgg ctgctgccag tggcgctttg tcgtgtcttc
     4981 ccgggttgga ctcaagacga tagttaccgg gcaaggcgca gcagtcgggc tgaacggggg
     5041 gttcttgcat acagcccagc ttggagcgaa cgacctacat cgagctgaga tacctacagt
     5101 gtgagctatg agaaagcgcc acatgtcccg taagggaaaa aggcggtcag gtatccggta
     5161 aagcgacacg gtcggaacag gagagcgcaa gagggagcga ttcaccggaa acggtgatga
     5221 tctttatagt cctgtcaggt ttcgccgcct ctggcttgag cgtctgtttt tgtgatgctc
     5281 gccagggggg cggagcctat ggaaaaacgg gtctggcgtt tttctctttg ctttcttacc
     5341 gcgtgcctgg atgccgttag cctgctagag tgcagcccgt agagtgtgtc atttaatcaa
     5401 ggtggtgtta tggcatggca cagtgtgtca caagcacaaa acatgacggg gaaaagtcgc
     5461 aggacccttt atcgtgatat ggcgtctggc cgcttgtcat ggcgaactaa tactagcggc
     5521 catcgcgaac ttgagacctc agaactgatc cgtgtttatg gcgagattaa aaacgatggc
     5581 acagctgaac ggcacaccgt gtcacagcct gatgtcacag tatccggtag tgacatcagg
     5641 gatgaattgc gtgccttacg gcaggaagtt gcagacctta aatctatgct tttgaggctg
     5701 gaacataaac aggaagagca gcctgaaact gcggctccgt ggtggcgttt ctggcagaaa
     5761 aaatctactt aacccttttc tctttttcgc ggctaaagtt gattacgtta tccggtagtg
     5821 tttcaggctt ggtggtcttt tgtgcctcga ttggcatagc actgtgtctt ctgttttgcg
     5881 tcaccagagc ccagataaag gcacagacaa agcggaaaga gaagtatatc ggtatgccta
     5941 tcacaaaggc gacggcggtt aaggccggga gcaagacccg gccaaacatc agagctaaca
     6001 gaacgccgac aacgattcca aatatcatgc gctgtagtag gggttagggt ctgccatacc
     6061 cgtgtattct gggtctgtca gatagttaat acgtgtgagc tgataagggt attcgccttt
     6121 cagaatcaca attggtcggg ttggtccaag cctcattact tcgtcaggtg tcagtaattc
     6181 cctcccagca aactgctgac ttgagccaga tgaacgacca cgattcattg agccgccacc
     6241 cccggaaact ccgccgccag agttgcggcc tgaattctgg gtttcaaatt caaccgtcga
     6301 tttacccaag gtgtcgctga cgtattttgc agtgtcatag tcgtctgtgc cgtagaacgt
     6361 cttcgctgag ttggccagaa acgtctgcca tttagggtag gcacttttca gctgtgacag
     6421 atcctgcagg aagacccaga aagacagccc gtagccacgt aacaggctta cggcatcctc
     6481 gattgatttc atatagccca gcacgttgaa ctcatcgagg atgaatgcga cattatgagc
     6541 tggtttcgcc ttcgattctg ttacagcgga gatcacagaa ccgataaaca gccttacaaa
     6601 tcgggtattt gctgcaatgc gattagctgg tagcacaaga taaactgtca tcggttcagc
     6661 tttgatggct gacaggttga agtctgagtg tgacagcgca gctgctatac gcgggtcgtc
     6721 cagaaatgct gtgtttttac gggcggtaga caatactgag ccacgttccc ggtctgccat
     6781 ccccatgagc gtgttggccg ctcgggcggg gatgccgaaa gcaacgtctt cacttgcggc
     6841 catatcggcc agcagggcta acaggttttc ttcatcactg gtgatcagtc gacgtagttc
     6901 cgcgagatta cgtcgggact catccggtaa tccggaaaca tgcagcatca ggccctgtaa
     6961 cagggttcgg gctgattcat caaaatgatt accgtcaccg cctttctctg ataccacaag
     7021 gctttcggcc agaactgcag attcagcgac gcaatccggg ctggtcacat cgagacgatc
     7081 cagaatgttc actgattctt ttatgccgct ggttataccg aatgggtcta ttacatggac
     7141 gctctgaccc atttcacgac ggcgacgacc tgtcacctcg gcgttttccc cttttggatc
     7201 aaagacaagt gctgaaccct gataagtcag aagattggga attactgcgc caatcccttt
     7261 tccgctgccc gtaggggcaa cagtcacaac gtgcccggtg aaacagaatt ttccatccac
     7321 tccgggcggt ggggacagtt ctcgtgccag ggtgaatccg gcttgctttt ccgttggtaa
     7381 caggtggtta ttttcccgca tttctgctgc ggttccccag cgtgatgagc cgtgagtgtg
     7441 atgtgatgcc gggttgacgg atgccgcgcc ggtttcctgc tttctgtcag catcgaggcc
     7501 agacacaaaa ccgcccatcc agcgaagccc ggaggccagt gcggggaaac gcccacagcg
     7561 agaggccagc cctccaagaa tttttttgct gcgggaataa atggcgaatg ttacggccag
     7621 cgctgccgcg tacaataccc atgatggtag gaaaggcatg aatatcagca tcagcgccag
     7681 tgccactgcg ggtaccgctg cggccatgac tgctttcact gatgccgtga aagcatccag
     7741 ccttacccgc cgtgcggtaa gtgcaccaat aataccaacg atgataagga acagcagaac
     7801 tggagcatat acctggctta tccgggcaag gaagccgccg aaatatcctt ccacgctgtc
     7861 acctttgaaa ttatgggctt tatcttcgac aatcttatct gattgcgcac gatactcttt
     7921 ctgggcttcg ttaccggcgt tctgtgccac gccttggata aagctctgtg cccgaacccg
     7981 agtctgataa accagctgtt gcgcgatacc tgcagctttg acctgtttat ccggttcagt
     8041 cagcatttga ctgacgttat cagtcagtcc gtcagcgctg gccggagcaa cgagccctgc
     8101 attctggtac aggtaattca gatcaccgcc gaaaagcgcc aggatgttac ccgcgcccag
     8161 taacgatgaa tttttttctg ccagtgcatg caggctatta atctggggag caatgttatc
     8221 gaacgtggat tttacggcgt tccagttctg atgatcgcgg gcgctgcgga tggcgttttc
     8281 tgcatctccc atcgcctgga aattttggcg ccactggtca taagtgtcat taacggcctg
     8341 acaccgcttt gtttcctgta gtctggcgat gttttttagc tgctcatctt ggatcattgc
     8401 ccagactgtc tcatttggat ggcagcgggt gaatgtttcg gccgctgcgt gccatactgg
     8461 caatagcatt gcgacgaaga aaatcccggc tagtatattt tttttcacgc taaatctcct
     8521 gatctgattt atcatccggt aataaataat tggcgtcact tattaccggg gttaaaagat
     8581 tcttttctgg cgtcaactgc ctttaaaccc atttcatcaa acagtgcccg gtctctgtca
     8641 tttttcagct cattgtccag aagactgacc aactgctgat aattccattc cccacgcttt
     8701 atttttgcta gtacggctcc accgattagg atttttcggc gggtatcctt cttgcgttct
     8761 tctgtctgct ctctttgctt taattgctgg atttgcgcct ttatctgagc ttccttctga
     8821 cgcaactc
//
