LOCUS       NZ_CP054284            34051 bp    DNA     circular CON 14-JUN-2023
DEFINITION  Escherichia coli strain IDR1900005638-01-02 plasmid p5E34, complete
            sequence.
ACCESSION   NZ_CP054284
VERSION     NZ_CP054284.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15086849
            Assembly: GCF_014169995.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34051)
  AUTHORS   Prussing,C., Snavely,E., Singh,N., Lapierre,P.,
            Lasek-Nesselquist,E., Mitchell,K., Haas,W., Owsiak,R., Nazarian,E.
            and Musser,K.
  TITLE     Nanopore MinION sequencing reveals possible transfer of blaKPC-2
            plasmids across bacterial species in two healthcare facilities
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 34051)
  AUTHORS   Prussing,C., Snavely,E., Singh,N., Lapierre,P.,
            Lasek-Nesselquist,E., Mitchell,K., Haas,W., Owsiak,R., Nazarian,E.
            and Musser,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUN-2020) Division of Infectious Diseases, Wadsworth
            Center, New York State Department of Health, 120 New Scotland Ave,
            Albany, NY 12208, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 15-OCT-2019
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 234x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/14/2023 05:23:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,590
            CDSs (total)                      :: 4,473
            Genes (coding)                    :: 4,368
            CDSs (with protein)               :: 4,368
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 47 of 105
            Pseudo Genes (incomplete)         :: 57 of 105
            Pseudo Genes (internal stop)      :: 24 of 105
            Pseudo Genes (multiple problems)  :: 24 of 105
            Pseudo Genes (short protein)      :: 3 of 105
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP054284.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..34051
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="IDR1900005638-01-02"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p5E34"
                     /geo_loc_name="USA:Maine"
                     /lat_lon="45 N 69 W"
                     /collection_date="2019-01"
                     /collected_by="Maine Center for Disease Control and
                     Prevention"
     gene            join(33755..34051,1..21)
                     /locus_tag="HS093_RS22520"
                     /old_locus_tag="HS093_22500"
     CDS             join(33755..34051,1..21)
                     /locus_tag="HS093_RS22520"
                     /old_locus_tag="HS093_22500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbM/KikA/MpfK family conjugal transfer protein"
                     /protein_id="WP_023223517.1"
                     /translation="MRKIIIASVVILASSYSVASFAKDPCKTLACMAAKSGGEFGSVGD
                     SDCSGAIADFFNIVKKNKHGFLPNHTADARKEFLMECSGAEQNTAAVSRVISMFGRIRK
                     G"
                     /name="TrbM/KikA/MpfK family conjugal transfer protein"
     gene            24..338
                     /locus_tag="HS093_RS22525"
                     /old_locus_tag="HS093_22505"
     CDS             24..338
                     /locus_tag="HS093_RS22525"
                     /old_locus_tag="HS093_22505"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023223516.1"
                     /translation="MKTKSIIFTAALLLTGCASQSQVDQLTKQVNTLRTDVYNLQIQQR
                     NDTQALQREVQKSRPIGVCYLDGQPYSQGAVIAGRICASNGVIVNGRPTEVSWQVYHRR
                     "
                     /name="hypothetical protein"
     gene            314..1039
                     /locus_tag="HS093_RS22530"
                     /old_locus_tag="HS093_22510"
     CDS             314..1039
                     /locus_tag="HS093_RS22530"
                     /old_locus_tag="HS093_22510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353823.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004519 - endonuclease activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="WP_224132007.1"
                     /translation="MAGLSPPLTTRVRGFGPVFYGTGSMDILSLFPSNVIALWAVSFAF
                     LAVLYWWKITARKRRHKRKQQQARRVLAKIQTLPAFGYKIAYLRKIDPFTFEELLLEGF
                     KSHGFRTIRNKRYTGDGGIDGQVIIGKHRYLIQAKRYRGHIALQHVQEFDTLLKRHNCR
                     GLFCHTGKTGAASKAVSFASERMEIISGQRLIDLLTPGSSFTIATAPQTMMRRTAATRG
                     TNIIVEEADKNTTDSMRVN"
                     /name="restriction endonuclease"
     gene            1205..1441
                     /locus_tag="HS093_RS22535"
     CDS             1205..1441
                     /locus_tag="HS093_RS22535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010428174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
                     /protein_id="WP_042041953.1"
                     /translation="MKFIESIKDKENLQRTYLMRFRRCSSLDTLEKVFDNLRDKLSGKE
                     LDAMMSASDHRRAEITHNTLWDKVPASAWKNVK"
                     /name="Hha/YmoA family nucleoid-associated regulatory
                     protein"
     gene            complement(1467..1904)
                     /locus_tag="HS093_RS22540"
                     /old_locus_tag="HS093_22520"
     CDS             complement(1467..1904)
                     /locus_tag="HS093_RS22540"
                     /old_locus_tag="HS093_22520"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_042041938.1"
                     /translation="MAISESSLGKQILEKINDHDRLNSKLLLKMKILNVLVFVILFAIG
                     DKFNELVICAIAFVVFFVLTGVYLKCKMVISSSLIFDYFERSAVDEGFRNFFMGLFNSK
                     RFLTIRDDRKKIHDYIYEQDKKFEELKKIETINHGMNGRSE"
                     /name="hypothetical protein"
     gene            complement(1913..2239)
                     /gene="mazF"
                     /locus_tag="HS093_RS22545"
                     /old_locus_tag="HS093_22525"
     CDS             complement(1913..2239)
                     /gene="mazF"
                     /locus_tag="HS093_RS22545"
                     /old_locus_tag="HS093_22525"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="WP_016240388.1"
                     /translation="MKRDYIPDAGDIVWMDFNPQAGHEQAGHRPALVLSPAVYNGRIGL
                     LLCCPMTTKIKGYPFEVRNGEGSAVLADQVKSLDWRERGAVKKGKVSAAVLAEVRSKLK
                     VLIG"
                     /name="mazF"
     gene            complement(2229..2486)
                     /locus_tag="HS093_RS22550"
                     /old_locus_tag="HS093_22530"
     CDS             complement(2229..2486)
                     /locus_tag="HS093_RS22550"
                     /old_locus_tag="HS093_22530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_042041940.1"
                     /translation="MLNFLKKIYSYLEFLGALIWVGVGLYSIWLGFSKLFKDNSISIGG
                     VEVISLLDTTPFAIMFIFCGVLIITFIWVGYISKRLKNEA"
                     /name="hypothetical protein"
     gene            complement(2498..2743)
                     /locus_tag="HS093_RS22555"
                     /old_locus_tag="HS093_22535"
     CDS             complement(2498..2743)
                     /locus_tag="HS093_RS22555"
                     /old_locus_tag="HS093_22535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000195442.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="WP_021536799.1"
                     /translation="MTQVIIKKWGNSPSVRLPSAIVKAASLNVDDVVELSVEDGAITIV
                     PVKTKEYSLDALMAGVTNENIHEEISFGSPVGKELL"
                     /name="AbrB/MazE/SpoVT family DNA-binding domain-containing
                     protein"
     gene            3195..3779
                     /locus_tag="HS093_RS22560"
                     /old_locus_tag="HS093_22540"
     CDS             3195..3779
                     /locus_tag="HS093_RS22560"
                     /old_locus_tag="HS093_22540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690619.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_021536796.1"
                     /translation="MKIGYARKSTKEQDTSLEYQIEALQKAGCEKWYAEQVSRGGNKRL
                     KTGTPELENCLKALRAGDTLVVWALDRLGGTLLQLLQLMNELRSKGIHFESLKERIDTQ
                     SPMGEMYYSLIAIFSNFEVARNHERTMAGLAAAKKKGRIGGRKPKLSPQDIKEINALVK
                     SPEITVKEIAARFKVSRPTIYRHITPDSNTD"
                     /name="recombinase family protein"
     gene            3812..4696
                     /locus_tag="HS093_RS22565"
                     /old_locus_tag="HS093_22545"
     CDS             3812..4696
                     /locus_tag="HS093_RS22565"
                     /old_locus_tag="HS093_22545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="WP_021536795.1"
                     /translation="MTDKPLPAKQIKTVNTGIAIKENPPKGDDMAFTHSILCQVGLPRS
                     KVDGREFMRKSGDAWLVVQAGWLDEGSGPVEQPIPYGSMPRLALAWVSSYALRKKTREI
                     PIGKSANEFLQLMGLSGGGRQYKTLREQMHALAACRLQLGFKGRSFNGQPVEQFDAWQK
                     DDQSGQLSLWPGVMTLSEGYFNGLIDSAVPLDNRALYALKGSALSLDVYTWLAHRLHRI
                     EGRPIKLHWMNLREQFAQEYMGKDPDKDFKKKFLPALREVLTVYPQAKVEKVTGGILLY
                     PSPPPIPFKEAKK"
                     /name="replication protein RepA"
     gene            5458..5877
                     /locus_tag="HS093_RS22570"
                     /old_locus_tag="HS093_22550"
     CDS             5458..5877
                     /locus_tag="HS093_RS22570"
                     /old_locus_tag="HS093_22550"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="WP_021536793.1"
                     /translation="MKKSLFSIILLATTYQASAEPCMVIFEHGQLITLPAAITQADQVK
                     GMSGENHSTGLILAWKTAEPRAVWMKDTPTPLTAAFIGADGVIQSIQDMQPNSDTAHSS
                     LHPTIAIIEMRTEKFKQFRLSKGDKIISSQCFPLK"
                     /name="DUF192 domain-containing protein"
     gene            6501..6770
                     /locus_tag="HS093_RS22575"
                     /old_locus_tag="HS093_22555"
     CDS             6501..6770
                     /locus_tag="HS093_RS22575"
                     /old_locus_tag="HS093_22555"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000779465.1"
                     /translation="MKLTTLLKKHFDIEEITDVDSTVNREVYTIWVYEKGEDCEPLLIL
                     KDAQDFMGVDGWLVGNIYSTLQHGLLLQHEELKTMIRNGEIKSR"
                     /name="hypothetical protein"
     gene            6859..7074
                     /locus_tag="HS093_RS22580"
                     /old_locus_tag="HS093_22560"
     CDS             6859..7074
                     /locus_tag="HS093_RS22580"
                     /old_locus_tag="HS093_22560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000111575.1"
                     /translation="MSVKDFTPTLEIKFHRRRWRIMVGRSSLASFRSEQDAIDALNKRR
                     SFYEYWAGSAGVQAENTEPVIVHVTY"
                     /name="hypothetical protein"
     gene            7264..8091
                     /locus_tag="HS093_RS22585"
                     /old_locus_tag="HS093_22565"
     CDS             7264..8091
                     /locus_tag="HS093_RS22585"
                     /old_locus_tag="HS093_22565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="WP_024147850.1"
                     /translation="MMLSSRFRSANILRSDRPLNDDQIMRYAPSIFATEAHESRSDRYA
                     YIATSEVLNGLRDNGFHPFMVCQTRVRNLDKREHTKHMIRLRHAGQINGTEAKEIILLN
                     SHDGSSSYQMLAGVFRFVCHNGLVCGDTVADIRIPHKGNVTERVIEGAFEVLDQFDNVE
                     NHMDAMKSVTLNALEKRIFADAALALRYDEKDGPAPIAASQLLEARRFEDRKDDLWTTF
                     NRVQENIIRGGLSARTKQGKSTRTRAVTGMDKDTSLNRALWILSERMNELKAA"
                     /name="DUF932 domain-containing protein"
     gene            8149..8628
                     /locus_tag="HS093_RS22590"
                     /old_locus_tag="HS093_22570"
     CDS             8149..8628
                     /locus_tag="HS093_RS22590"
                     /old_locus_tag="HS093_22570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="WP_000168313.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKQ
                     TGEMKEQTEWHRVVLFGKLAEVAGEYLRKGSQVYIEGQLRTRKWTDQAGVEKYTTEVVV
                     NVGGTMQMLGGRPQNASQPGNQPQAGQQFSGQPQATGTAAAAGNEPPPFDDDIPF"
                     /name="single-stranded DNA-binding protein"
     gene            8695..8997
                     /locus_tag="HS093_RS22595"
                     /old_locus_tag="HS093_22575"
     CDS             8695..8997
                     /locus_tag="HS093_RS22595"
                     /old_locus_tag="HS093_22575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrfB-related DNA-binding protein"
                     /protein_id="WP_000257688.1"
                     /translation="MAVERISPEEWQRIAPALRTCSQVTIDMAYAVLVDGRKQVDVAKE
                     FDRSKQTVNAAIRRVTAIFNEVIPEDEQLEFVQVWLPPELAKQVKEMAKPYQNKN"
                     /name="TrfB-related DNA-binding protein"
     gene            9012..9353
                     /locus_tag="HS093_RS22600"
                     /old_locus_tag="HS093_22580"
     CDS             9012..9353
                     /locus_tag="HS093_RS22600"
                     /old_locus_tag="HS093_22580"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021536824.1"
                     /translation="MAKVRFENSLNKMIFEIRGYESFSEMETALLDFCDETMGANHPDI
                     VVEYPVYYKHFINDKISYEHIGYVNLGIDQDDGSCYTIEHLTLDRKTLKNHWHPFYFYK
                     GECEYGFKN"
                     /name="hypothetical protein"
     gene            9501..9641
                     /locus_tag="HS093_RS22605"
                     /old_locus_tag="HS093_22585"
     CDS             9501..9641
                     /locus_tag="HS093_RS22605"
                     /old_locus_tag="HS093_22585"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_153302164.1"
                     /translation="MPVGALAAARPTPQHPDKSRPARRYAPARPLNRAVVTGHCSGGGI
                     V"
                     /name="hypothetical protein"
     gene            9995..10417
                     /locus_tag="HS093_RS22610"
                     /old_locus_tag="HS093_22590"
     CDS             9995..10417
                     /locus_tag="HS093_RS22610"
                     /old_locus_tag="HS093_22590"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021536823.1"
                     /translation="MSAYVVSDKAISTIVKTLVLTGTLQPVEAVSFGQMMLNLNTHSVN
                     VRYQESSPAHAFEYSEPELNINDPKTQIQVIVCIDEYEYQSCEFAEYYETMVHTVLKAI
                     KSALHEAYTETLPNPARWKAKKSYELPGYSEAEWSL"
                     /name="hypothetical protein"
     gene            11277..11453
                     /locus_tag="HS093_RS22615"
                     /old_locus_tag="HS093_22595"
     CDS             11277..11453
                     /locus_tag="HS093_RS22615"
                     /old_locus_tag="HS093_22595"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_156649898.1"
                     /translation="MASPSSASNGAIVPVGALAAARPSPQHPDKSRPARRYAPARPLNR
                     AVVTGHCSGGGIV"
                     /name="hypothetical protein"
     gene            11805..12092
                     /locus_tag="HS093_RS22620"
                     /old_locus_tag="HS093_22600"
     CDS             11805..12092
                     /locus_tag="HS093_RS22620"
                     /old_locus_tag="HS093_22600"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001098466.1"
                     /translation="MNTTLVVLVLAITGALYLPWRLVRDGYKELVQMCLFCSCIVTLAA
                     VYAVLVNLDTPHPFMPMFIAVTAFGAAFVADCALSLWLYQRKANAKTANH"
                     /name="hypothetical protein"
     gene            12147..12389
                     /locus_tag="HS093_RS22625"
                     /old_locus_tag="HS093_22605"
     CDS             12147..12389
                     /locus_tag="HS093_RS22625"
                     /old_locus_tag="HS093_22605"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000526841.1"
                     /translation="MGNIILMAEKAKGAIDEEAEVYEFEGMDDLIQFRKKFPEQMKYEY
                     HYILSGGTKNFRHIALVEANHFKQFKKLVNLYQDR"
                     /name="hypothetical protein"
     gene            12542..12697
                     /locus_tag="HS093_RS22630"
                     /old_locus_tag="HS093_22610"
     CDS             12542..12697
                     /locus_tag="HS093_RS22630"
                     /old_locus_tag="HS093_22610"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021536821.1"
                     /translation="MKKFICNRNGKAVFQEGDRELVAAYQMKSSPVPVTPRKLKAEMEA
                     IARTAR"
                     /name="hypothetical protein"
     gene            12770..13027
                     /locus_tag="HS093_RS22635"
                     /old_locus_tag="HS093_22615"
     CDS             12770..13027
                     /locus_tag="HS093_RS22635"
                     /old_locus_tag="HS093_22615"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021536820.1"
                     /translation="MCIDHVLTFPVPLEDASLLADYCGFEVYPTYSTNRADETPRFSVV
                     ALHRHKARLETLAVADTEKSAHAFRDMAEITAAYYLHFRR"
                     /name="hypothetical protein"
     gene            13032..13220
                     /locus_tag="HS093_RS22640"
                     /old_locus_tag="HS093_22620"
     CDS             13032..13220
                     /locus_tag="HS093_RS22640"
                     /old_locus_tag="HS093_22620"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001001010.1"
                     /translation="MNDYLSLMEQARQAEDLRDWKTAAQLYTQAMAVSPKARTMRQRRE
                     RLNLQQRIDFCERNART"
                     /name="hypothetical protein"
     gene            complement(13261..14031)
                     /locus_tag="HS093_RS22645"
                     /old_locus_tag="HS093_22625"
     CDS             complement(13261..14031)
                     /locus_tag="HS093_RS22645"
                     /old_locus_tag="HS093_22625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="WP_021536818.1"
                     /translation="MIIPTQETYDELQRAYEFFNQRLFESALPPCLITLQREKRTYGYF
                     SNGRFVGRSTGQIVDEIAMNPVYFSIRTIRDTLSTLVHEMVHQLQQHTGKPGRRGYHNK
                     EWAAMMERIGLIPSDTGQPGGKRVGESMSHYIEDGGAFDTACKELLTTEFKLSWLDRFP
                     PHKPTPPTPTGADGKGYVDDEDYEDEGYGDDGELDDEITQALSLVNPPPDTPVNKSNRT
                     KYTCPKCHTNVWGKPGLILYCGGEHCGKAEYQPT"
                     /name="SprT-like domain-containing protein"
     gene            complement(14245..14613)
                     /gene="stbC"
                     /locus_tag="HS093_RS22650"
                     /old_locus_tag="HS093_22630"
     CDS             complement(14245..14613)
                     /gene="stbC"
                     /locus_tag="HS093_RS22650"
                     /old_locus_tag="HS093_22630"
                     /inference="COORDINATES: protein motif:HMM:NF041291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein StbC"
                     /protein_id="WP_015345231.1"
                     /translation="MEKQPDKLDILMDWFLGDAKEITSKLESVTTSVTETVERLDKASD
                     EIKKSSEVAKTEVIASQRELVNTLKKEQKVSDQFFERLAGAQTGFSQALIRQILIITVV
                     CSFVGGALGAAAITFLLH"
                     /name="stbC"
     gene            complement(14615..15325)
                     /gene="stbB"
                     /locus_tag="HS093_RS22655"
                     /old_locus_tag="HS093_22635"
     CDS             complement(14615..15325)
                     /gene="stbB"
                     /locus_tag="HS093_RS22655"
                     /old_locus_tag="HS093_22635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011279206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StbB family protein"
                     /protein_id="WP_000686338.1"
                     /translation="MKIAVMNYSGSVGKTIISSYLLYPRMAGAKFFAIETINMSAADLG
                     VDEVMSLTGDNFGQLVEEIVFEDNAIVDIGASNVERFLSTMTKYEGAIDEFDKYLIPVT
                     PDDKAWQESIKTALALSAAGVPKSKIVLLPNRIKSTPQEDIASVYEWAKDSKKASIHKD
                     AAVFESEIYEYLAYHKISFEELLAEDPETFKAKAKTCTDADERAAAARRYRWMKLALPV
                     KRNLDRTFEILTAE"
                     /name="stbB"
     gene            complement(15338..15772)
                     /locus_tag="HS093_RS22660"
                     /old_locus_tag="HS093_22640"
     CDS             complement(15338..15772)
                     /locus_tag="HS093_RS22660"
                     /old_locus_tag="HS093_22640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003032490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021536817.1"
                     /translation="MKKDLLLEPRNLAAAVRARLDEIEGLLDKGISRQEIAQLLDSEYS
                     FSVTVKALDMALYRARLKRKKGGLHNPESTVNTGLHNPKEQEKAEPVETGLHNPEPEKL
                     VEDMGGNKEFLSDTDFKKIGDEAKKVATSFKGSVGNKKGF"
                     /name="hypothetical protein"
     gene            16259..16606
                     /locus_tag="HS093_RS22665"
                     /old_locus_tag="HS093_22645"
     CDS             16259..16606
                     /locus_tag="HS093_RS22665"
                     /old_locus_tag="HS093_22645"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001139329.1"
                     /translation="MPSISLRVTEAQKIDLEYRSEGNVSEYIKQQLFGQKESASEVIAR
                     LDKLLERSSSEQQAAGRDRYIEAMLIEVLLILRQVSKADAQKVAGGEIRRLGLEPWSAE
                     LAQEREEQSHE"
                     /name="hypothetical protein"
     gene            16599..18176
                     /locus_tag="HS093_RS22670"
                     /old_locus_tag="HS093_22650"
     CDS             16599..18176
                     /locus_tag="HS093_RS22670"
                     /old_locus_tag="HS093_22650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="WP_021536815.1"
                     /translation="MNEADKGKICLGALLFTPLVTWFIAAKALYGFTPKDARERLVLLI
                     QQTFDLWPLWGALLVGEIIAIIGLIIFFKFNHQIFKGAPFKKIYRGTRLVSQRALASIT
                     KEREPQITIADIPIPTKAEGTHISIAGATGVGKSTIFAEMMKCCLMRGKMQRVASNKDR
                     MIILDPDGDFLSRFYKKGDKILNPYDARTEGWVFFNEIKSDFDFERFGVSIVPTAKDSQ
                     TEEWNGYGRLLFTEVSRKVFNTSRNPTMEEVFYWTNEVPLEELEEYVRGTKAQALFAGS
                     GRAVGSARFVLSNKLAAHLKMPAGNFSIREWLEDPKGGNLYITWTDEMREALKSLISCW
                     TDSIFSIVLGMSKSNSRKIWTFIDELESLDYLPSLRDALTKGRKKGLRVVTGYQSYSQV
                     ISIYGSDVAETLLSNHRTSVVMAAGRLGERTLEFVSKSLGEIEGEREKTGISRRFGQLG
                     TKNTNDDHKRERAVTPTEIATLDDLTGYIAFPGNLPVAKFETHHVNYTRSNPVPGVVLR
                     ESTAFAGM"
                     /name="type IV secretion system DNA-binding
                     domain-containing protein"
     gene            18179..21088
                     /gene="mobF"
                     /locus_tag="HS093_RS22675"
                     /old_locus_tag="HS093_22655"
     CDS             18179..21088
                     /gene="mobF"
                     /locus_tag="HS093_RS22675"
                     /old_locus_tag="HS093_22655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932317.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobF family relaxase"
                     /protein_id="WP_021536814.1"
                     /translation="MLNITTITRQRVDKVVDYYADGADDYYAKDGNAMQWQGAGAEELG
                     LTGEVEQKRFAKLLDGKVTDDISVMRKTANSESKERLGYDLTFSAPKGVTLQALVNGDK
                     RIIEAHDRAVTKAIEEAERLALGRFTVNKKTHVENTNKLIVGKFRHETSRELDPDLHTH
                     AFVMNLTKTSDGKWRSLTNDGIVNSLTLLGNVYKSELARELELAGYNLRYDRNGTFDLA
                     HFSQEQIAQFSQRSQQIEAELAKNGLSRETASHEQKNAAALKTRKSKGEVDRDVLYEGW
                     KNRAKELQIDFDSREWKGAANDDIARNTQPAALEKPIEYQADKVMTFAIRSLTERQSVI
                     TERELMDVAMKHGYGRLTLDDIRAARERAITSGNLIKEEATYAAATTNKKQQNVAMTRE
                     QWVTELVKAGRNKDEARKLVDKGITNGRLVQQKPRFTTQLAQQRERNILKMEREGRGKI
                     QTPYTREFSEGWLASRTLKPEQLKAVMGIIHTPNQFISVHGFAGTGKSYMTKSAADFLK
                     EQGVHVTSLAPYGSQVKALQAEGLESRTLQSFLRASDKKIGPGSVVFIDEAGVIPARQM
                     EETMRVIRDAGARAVFLGDTKQTKAVEAGKPFEQLIKAGMETAYMKDIQRQKDPELLKA
                     ALNAAEGKIKASLTHVTSIVEEKNHDQRYRRIVGDYVAMPPTDRANALIITGTNDSRKK
                     INEYIQAELGLKGQGINYPLLNRLDTTQAQRQHSKYYEKGAVIIPERDYSNGLKRGAVY
                     TVLDTGPGNKLTVSDGSGDTIAFSPARFSKLSVYSVEKTELAVGDQVRITRNDAHLDLA
                     NGDRFKVKGVQSGEVLLENEKGRLVKIDANKPMYLGLAYASTVHSAQGLTCDKVFINMD
                     TRSLTTAKDVYYVAVTRAKHEAVIYTDDEKKLDKAASREGFKTAALELEQLKRYAKEQQ
                     HDAREKGRDKTEPTQEKDNAKRKGKGHDKNNDERAFTSL"
                     /name="mobF"
     gene            complement(21366..21560)
                     /locus_tag="HS093_RS22680"
                     /old_locus_tag="HS093_22660"
     CDS             complement(21366..21560)
                     /locus_tag="HS093_RS22680"
                     /old_locus_tag="HS093_22660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000529710.1"
                     /translation="MGQGINTLSLELDDKEALALAQFVKRLTWSDLRGCAVDDDEAYVI
                     KDAVDKLQRAMAEEGFSPR"
                     /name="hypothetical protein"
     gene            complement(21572..22054)
                     /locus_tag="HS093_RS22685"
                     /old_locus_tag="HS093_22665"
     CDS             complement(21572..22054)
                     /locus_tag="HS093_RS22685"
                     /old_locus_tag="HS093_22665"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="WP_042041949.1"
                     /translation="MLDMRLGNSGDIPFIMNLVRDGITSGAFFYAEGNGLETVRSELQS
                     VTQQYRDNIPVGDYLFVFAEKLTRQPVGFSLLTGRNDFAGRASHLEIRMFAVAADEQGK
                     GIGKSMLASIIEATPGHRLEASCLPPATKMMRLLDGSGFKLIHETQGGKKTYRFTD"
                     /name="GNAT family protein"
     gene            complement(22067..22621)
                     /locus_tag="HS093_RS22690"
                     /old_locus_tag="HS093_22670"
     CDS             complement(22067..22621)
                     /locus_tag="HS093_RS22690"
                     /old_locus_tag="HS093_22670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013036432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="WP_000616063.1"
                     /translation="MIQRLKKRNFLFKAALVAAVLYGASAPSLFAASAQVGFSPEGTAQ
                     QLVINVIGSAKDNIRMMAYSFTAPDIMKALIAAKRRGVDVKIVVDENGNTGRASRAAMN
                     LVTNAGIPLRVNSNYKIQHDKVIIVDGRHVETGSFNYTASAEKYNSENAIVMWDAPELA
                     GQYLKHWQSRWNQGRDFTPSY"
                     /name="phospholipase D family protein"
     gene            complement(22584..23615)
                     /gene="virB11"
                     /locus_tag="HS093_RS22695"
                     /old_locus_tag="HS093_22675"
     CDS             complement(22584..23615)
                     /gene="virB11"
                     /locus_tag="HS093_RS22695"
                     /old_locus_tag="HS093_22675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138004.1"
                     /GO_component="GO:0043684 - type IV secretion system
                     complex [Evidence IEA]"
                     /GO_process="GO:0044097 - secretion by the type IV
                     secretion system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="WP_042041926.1"
                     /translation="MTELTSNIQDIRQRPRREQGVWLTLEPLQRYLSDPDVWELRINKY
                     QQVVCETSKGRIFHDDEKITAEYISRLTDTLCGYNGLPKESVNNVLLPDGSRGIICLPP
                     AVLEGTVAIAFRKHLPVDKSLEQLRDEGRFTGWKRKVQGEVALQPFEQELLELLDKDDL
                     VSFFKIAVRNKLNIVVSGSTGSGKSTFTRSLLREVPYTERIVVSEDVHEIGSELHHEMV
                     YMLYGDQDGRLAPAVSLKACMRLSPDRIMMTELRDDAAWDYINSLNTGHPGGITSTHAD
                     DAKSTFSRIALLIKQTGVGQTLDYDVIMKTLYTTIDVVVYMEHRQIVEVLYDPAFKKAQ
                     LSL"
                     /name="virB11"
     gene            complement(23608..24762)
                     /gene="virB10"
                     /locus_tag="HS093_RS22700"
                     /old_locus_tag="HS093_22680"
     CDS             complement(23608..24762)
                     /gene="virB10"
                     /locus_tag="HS093_RS22700"
                     /old_locus_tag="HS093_22680"
                     /inference="COORDINATES: protein motif:HMM:NF038091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein VirB10"
                     /protein_id="WP_001224296.1"
                     /translation="MRKDREENLDYLDEEDTGIEDERQGFSGSPKKRIAGARAFVGLMV
                     LIGSAMVGWFIYNQMHVKKLADDPKKDKEQVMTSSLPRQSFDDVPPTIDNTPKNSVVPP
                     LQTTQESQQQVARQTLNNQKKELSPEEKIMRARLGVSFNGVDPEKQATTAKEQERRSGQ
                     DNTETSSNSQALASRMKPARMAVAQASVMPHPNLTIAQGTMIPCGTNTELDTTVPGQVK
                     CTVSRDVYSADGTVKLIDKGAVVTGEQNSGIQNGQNRVFVLWSRLRNPDQTIVNLDSSG
                     TNALGSAGVPGQVNSHFWERMGDAIFFSVLTDGMKAGIASINNNNSDVTFNTTENSTDQ
                     LATEALRAKINIPPTLYAAQGDAVSIYVARDLDFSTVYGLEMND"
                     /name="virB10"
     gene            complement(24759..25646)
                     /locus_tag="HS093_RS22705"
                     /old_locus_tag="HS093_22685"
     CDS             complement(24759..25646)
                     /locus_tag="HS093_RS22705"
                     /old_locus_tag="HS093_22685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbG/VirB9 family P-type conjugative transfer
                     protein"
                     /protein_id="WP_000744355.1"
                     /translation="MKKQLIAGLILVSLGLSGQALALEVGKGSPYDYRIKSVVYNPVDV
                     VRIDSVAGVATHIVVAPDESYVTHAFGDSNSYAFTHVENNMFIKPKGEVPSDTNLVLVT
                     NKRTYNIVLHYVGDYTTKDAQGNVKANFIKTPWSMKNATLQLTYLYPFEDMKKANKTLE
                     SNRVKKALKDLQNGPYNLLYKMSDEDEYRTVAPVNVWDNFRFTYFKFPANADLPVVFYI
                     GDDGKEHTVNQHPAGEYNNIVVAETVAKEWRIRYGNKVVGVLNMNYNPNLGAISTGTTS
                     PDVKRVKKGEGDKS"
                     /name="TrbG/VirB9 family P-type conjugative transfer
                     protein"
     gene            complement(25649..26365)
                     /locus_tag="HS093_RS22710"
                     /old_locus_tag="HS093_22690"
     CDS             complement(25649..26365)
                     /locus_tag="HS093_RS22710"
                     /old_locus_tag="HS093_22690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_000914645.1"
                     /translation="MLKKNKHQAISREDLNKYHEETQGLERDMVNEILNSRSRAWKIAT
                     AFFVFAVVSMITAVGVIIRFAQPLPAYLTTINKDTGEVSQVKITRDEATYGDVIDQYWI
                     SQFVIHRESYDYNSIQVDYDAMSLMASGDVADEYLSMFKGPNRIDKRLGDSERTTVHIN
                     SVITDREHGVATVRFTTQQRIRQRPNPEPPRYWIATIAYEYKALPMTAQQRYINPLGFR
                     VTSYRKNAENVGAVGG"
                     /name="type IV secretion system protein"
     gene            complement(26368..26538)
                     /locus_tag="HS093_RS22715"
                     /old_locus_tag="HS093_22695"
     CDS             complement(26368..26538)
                     /locus_tag="HS093_RS22715"
                     /old_locus_tag="HS093_22695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021536809.1"
                     /translation="MVHFFKYTAMALIGLLLAACSSSPTPPPEPNMKKLVDVNKTYPAE
                     LYRADAANNGE"
                     /name="hypothetical protein"
     gene            complement(26633..27640)
                     /locus_tag="HS093_RS22720"
                     /old_locus_tag="HS093_22700"
     CDS             complement(26633..27640)
                     /locus_tag="HS093_RS22720"
                     /old_locus_tag="HS093_22700"
                     /inference="COORDINATES: protein motif:HMM:NF016491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_170879277.1"
                     /translation="MDTVTKEFVASNLGVVISTISPVIGLALICKLTLDGLFTLARPHG
                     EPLSSLIKKFIVWSMIISFASAGGLYQTQLADAAMKIPDEFSSVLVINGSSGGTEDAMG
                     NNIDKAIHDGMQVARTAFDNADIYSGSGLASLFLACAVIISTVLICGIGAGLVLMAKLL
                     LAITVCFGPIFIFCLLFKPLYGMFNKWLGSMVNYGLVTILLSMLFGLMIKFYQKALDAV
                     TASGADSSLLGPVFACLLIMIVSYLILMRIPEIASRWGDGISAGIQELIPNAGGGGRGA
                     GGGGNQPGGQKGATGAGGGTGAKTAAATAAGGPGAGAATAAGSQMQGFAKGSRR"
                     /name="type IV secretion system protein"
     gene            complement(27695..27931)
                     /locus_tag="HS093_RS22725"
                     /old_locus_tag="HS093_22705"
     CDS             complement(27695..27931)
                     /locus_tag="HS093_RS22725"
                     /old_locus_tag="HS093_22705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="WP_000733633.1"
                     /translation="MKKLILPLALFLLAGCDSEHPVSWYKEHDQERKAMVEKCRDDAAL
                     KVKPDCQNALEAEKDLKVFGKSDDGLSMPKVTP"
                     /name="EexN family lipoprotein"
     gene            complement(27935..28642)
                     /locus_tag="HS093_RS22730"
                     /old_locus_tag="HS093_22710"
     CDS             complement(27935..28642)
                     /locus_tag="HS093_RS22730"
                     /old_locus_tag="HS093_22710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_023187073.1"
                     /translation="MKKTLASLVLALAISPVAVNAQLVVNDPANLAQALQQVTLMTQQI
                     EQLKSQLLKQEQTLASLTGSSGIGNLLNGDISTLKGNLPSDWTQVYSDAMNSSSGITSS
                     AQSMLKEFQSQIDGMNRSEAMSFIKQKLAEKGAYDRVMAQNAYNNEMAELNNIQELTNQ
                     IDSTTDMKQIADLQARIQTAQGAIQGEQTKLQLMAMLQTSQDKILQEQKQYAQKRFVLG
                     ADGEDLSMPNVTE"
                     /name="type IV secretion system protein"
     gene            complement(28652..31282)
                     /locus_tag="HS093_RS22735"
                     /old_locus_tag="HS093_22715"
     CDS             complement(28652..31282)
                     /locus_tag="HS093_RS22735"
                     /old_locus_tag="HS093_22715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
                     /protein_id="WP_042041932.1"
                     /translation="MAVAEMKKRGVKDFKKEPSSLKYIPYSYHVTPHVISTVNGEYLSI
                     FKIRGRTHDCASDAELIKWHADLNQLLKGIGSEHVKLWTHLHHREINNYPDTHYNMTFP
                     RMVNEYYRDQFDDTPMMVNDLYLTVVYNPVGDAAQKMLAKFDKPSANELREMQADAISA
                     LEDICEQVMNSLRPYGIDRLGYYFRDKRGNVIPEQKKDDAEDDEDIIDLREDLLADVEY
                     QDVQEDKEVFSGTQHTYSKALEWLYYLVNGEWAPVPVCRGRIREYLATTRPTSSFWGDG
                     IEIRGTDERRYTSAVEIRDYDSDTEPGQINILMEAPFEFVLTQTYCCMSQQAARTYLTN
                     QQKALLETGDPSFTQINQMNDAVDGVVSRTFIMGWHHLTVHTFGKTLKEARSNAGVARN
                     MLNNCGLIASQVGLASEAAYYAKLPANQHLSPRPCPLNSWNFLCFSPFHNFMSGKANNN
                     PWGPALTLFKTVANTPLYMNLHVTPEDEMSFGKRPLAHTLMLGRSGAGKTTLLNFLLTQ
                     AMKYNPRIFAFDRDQGMMPMIKALHGHYTVLKEGVASGWAPLQMPGTKVNIAFCKRLMR
                     LCAEITKGSVLDADQVNDLNRAVDALMDENSLIPTDLRTVDMLIQQMPNPYQTAGSKQT
                     LASLLVPWTRGHENGWLFDNDSDSLNLGEYDIYGFDLTDFIVADNEIPPATRTPLLMYL
                     NFRVRQSIDGRRRVIQVFDEFHQYLDDPIMSVEVKRGLKTDRKKDAVYVFSTQEPNDAL
                     DSSIGKTIMQQCVTKILLENPDADYEDYVTRLKLTPAEFDALLSIPENSRQFLVKQGSQ
                     SAMAQFNLAPRVKTPENFRKLDKILSILSGETQNAETLAKIIEQIGDDNPDKWLEPYWK
                     SVLAA"
                     /name="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
     gene            complement(31282..31596)
                     /locus_tag="HS093_RS22740"
                     /old_locus_tag="HS093_22720"
     CDS             complement(31282..31596)
                     /locus_tag="HS093_RS22740"
                     /old_locus_tag="HS093_22720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="WP_001053888.1"
                     /translation="MNLSGKYPLFKGATRLPTFAGVPRTVIIATFIFCATLFMTIHLWA
                     VVLFGLLWFIEFCISKHDDRMFRIIWLAIMTKGLNMINSPFTKLWGGSSYSPTDYGDKK
                     "
                     /name="VirB3 family type IV secretion system protein"
     gene            complement(31648..31938)
                     /locus_tag="HS093_RS22745"
                     /old_locus_tag="HS093_22725"
     CDS             complement(31648..31938)
                     /locus_tag="HS093_RS22745"
                     /old_locus_tag="HS093_22725"
                     /inference="COORDINATES: protein motif:HMM:NF016820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="WP_023223518.1"
                     /translation="MNTAIKRFFPVAMFMMVALCVPELALAAGADTGESSLSSVQSWMM
                     TWIPIAATVLIIVSAIAWIAHMLPASWAVRIVIGLIVVGSASYLVGLFGLS"
                     /name="TrbC/VirB2 family protein"
     gene            complement(31922..32239)
                     /locus_tag="HS093_RS22750"
                     /old_locus_tag="HS093_22730"
     CDS             complement(31922..32239)
                     /locus_tag="HS093_RS22750"
                     /old_locus_tag="HS093_22730"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001267114.1"
                     /translation="MRVYLILLKQLEEVFNQNKVVRIAIQRMDKKKWIPLFDVKNSEGG
                     VITCSLRVQSGEGVRTWADLRILADMLRDDFNAREVGLFLDEPDFIFPDDGVLDKDEYS
                     D"
                     /name="hypothetical protein"
     gene            complement(32262..33002)
                     /locus_tag="HS093_RS22755"
                     /old_locus_tag="HS093_22735"
     CDS             complement(32262..33002)
                     /locus_tag="HS093_RS22755"
                     /old_locus_tag="HS093_22735"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing protein"
                     /protein_id="WP_042041934.1"
                     /translation="MKQVVALFPLLIAGLLDATPANAELSAPVMALAKRCAPEVHPLTI
                     GYLVSHESRNNPYAINVNVPKGTKSPLARQPRSLDEAVKTTKWLTAHGYNFDVGLGQIN
                     SANFPKLNVSAADLFNGCKNLEAAQYVLNYCYQLATKEYAPGQSALRHALSCYNTGSLT
                     AGFTNGYVSKVVAQIGQSKPVIPALKADDGSTDVESVDTAIDAVPVDAADGEEQPKDAG
                     MTDAFSGSDKDAFGASDGDAFRQK"
                     /name="lytic transglycosylase domain-containing protein"
     gene            33065..33301
                     /locus_tag="HS093_RS22760"
                     /old_locus_tag="HS093_22740"
     CDS             33065..33301
                     /locus_tag="HS093_RS22760"
                     /old_locus_tag="HS093_22740"
                     /inference="COORDINATES: protein motif:HMM:NF013014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="WP_021536804.1"
                     /translation="MSRQDEKKHKTEQERETSVPKRKIIFVPLQSEKSPKGEPRYRDPA
                     NPFNTWVGRGKRPDWLKKYLDEGRELSEFEIVE"
                     /name="H-NS histone family protein"
     gene            33313..33750
                     /locus_tag="HS093_RS22765"
                     /old_locus_tag="HS093_22745"
     CDS             33313..33750
                     /locus_tag="HS093_RS22765"
                     /old_locus_tag="HS093_22745"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023187074.1"
                     /translation="MSKIIDFPTGKKREKRAKAIRARRNKEAQQRSEITPSQFWWVKKI
                     KLLTVIKQSARFLLIYLGLLTADILIYLLFILGKLLKFFTMIGIIAVLLFLVVEYRNDW
                     HYTQSAFHAAAIFVGLIALRIGHDKLLNGLIRLKSYLSRKG"
                     /name="hypothetical protein"
     Typing          3813..4696
                     /name="IncN"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.396"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          18180..21085
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.69"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          16600..18173
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="98.476"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(24763..25646)
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(25653..26365)
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.44e-159"
     Typing          complement(26637..27676)
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="93.66"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(28656..31282)
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="97.831"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggacgtatca ggaaaggtta aatatgaaaa ctaaaagcat catcttcacc gcagccctct
       61 tactgaccgg ctgcgccagc cagtcacaag ttgaccagct aaccaagcag gtgaatacgc
      121 tcagaacaga tgtgtataac ctacagattc aacagcgcaa tgacacacag gcgcttcagc
      181 gcgaagtaca gaaatcgcgc cccatcggag tttgctatct tgacggccaa ccctattcac
      241 aaggggcggt cattgctggc cgcatttgcg ccagtaatgg tgtgatcgtc aatggcagac
      301 cgacagaagt ttcatggcag gtttatcacc gccgctaaca acgcgcgttc ggggcttcgg
      361 ccccgttttt tatggtacag gtagcatgga tatactttcc cttttcccct ctaacgtaat
      421 cgcattgtgg gccgtgtcat ttgcttttct ggccgtcctg tattggtgga agatcaccgc
      481 cagaaaacgc agacacaaac gcaagcaaca acaagcccgg cgtgtactcg caaaaattca
      541 gaccctaccg gcgttcggct acaagatcgc ttatctcagg aagatagacc ctttcacctt
      601 tgaggaactg ttactggagg ggtttaagtc gcatggcttc agaactatca gaaacaaacg
      661 ctataccggt gacggaggca ttgacggcca ggtaataatc ggaaaacatc gctatctcat
      721 ccaggccaaa cgctatcgcg gccatattgc tttacagcac gttcaggagt tcgatacgtt
      781 gcttaaacga cataactgtc gcggtttgtt ttgccatacc ggaaaaaccg gcgcagcgtc
      841 aaaagctgtt agcttcgcca gtgagcggat ggagattatc agcggccagc gcttgattga
      901 tttactcacg cccggcagct ccttcaccat cgcaaccgca ccgcagacaa tgatgaggcg
      961 taccgcagca acgcgaggaa cgaacatcat tgtcgaggaa gcggataaaa atacaaccga
     1021 tagtatgaga gtcaattaaa tgaagtcagt aactatagaa gcaaaaactt ttgctgaaat
     1081 gttaggaata acagaaggtg agttaatctt cgccattaag acaactggca catttaaaaa
     1141 caaaaccatt ccacaacctc atgagccaca taaatcgaat agcagatttt tatattctga
     1201 cgtaatgaaa tttatcgaat caataaaaga caaagaaaac ttacaacgaa catacttgat
     1261 gcgtttccgt cgatgcagct cgttggacac tcttgaaaaa gtatttgata acttacgcga
     1321 taagctatca ggtaaagaac tggatgcaat gatgagcgcc tcagaccatc ggagagccga
     1381 aatcactcat aatactttat gggataaagt tccggcttcc gcatggaaaa atgtaaaata
     1441 aaaaaagccc gctaacgggc ttttttttac tcactacgtc cattcatacc atgattaata
     1501 gtttctattt tcttcaattc ttcaaacttc ttatcttgct cataaatata atcatgaatt
     1561 ttctttctat catcccggat cgtaagaaac cttttgctgt taaataaccc cataaaaaag
     1621 tttctaaacc cctcgtcaac agcactacgt tcgaaataat caaatattaa cgacgatgaa
     1681 ataaccatct tgcacttcaa atacacaccg gttagcacaa agaaaaccac gaaagcaatc
     1741 gcacatataa ctagttcgtt aaacttatct cctattgcaa aaagaataac gaacacaagc
     1801 acgtttaaaa ttttcatttt taaaagcaat ttagaattta aacgatcatg atcatttatc
     1861 ttttcaagta tctgcttgcc tagagatgat tcgctaatag ccataataat acctatccaa
     1921 tcaatacttt taatttagac ctgacttcag ctaacacagc cgcagacact ttacctttct
     1981 ttacggcccc ccgttcacgc caatcaagac ttttcacctg gtcagcgagt acagcactcc
     2041 cctctccgtt ccgcacctcg aatggataac cttttatttt cgttgtcata gggcagcaca
     2101 acaacagccc tattctgccg ttatataccg caggacttaa cactaaagct ggtctatggc
     2161 ccgcttgttc atgcccggct tgcggattaa aatccatcca tacaatatca ccggcatcag
     2221 gaatataatc acgcttcatt ttttagtctc ttacttatat acccaaccca tataaatgta
     2281 atgatgagca ccccacaaaa aataaacata attgcaaacg gagttgtatc aagtaaagat
     2341 ataacctcaa caccaccaat agagattgag ttgtctttaa ataatttact aaaccctaac
     2401 cagatggaat acaacccaac accaacccaa ataagagcac ctaaaaactc caggtagcta
     2461 taaatttttt tcaaaaaatt cagcataacc cctccaatta taaaagttct ttacccacag
     2521 gagatccgaa agagatttcc tcatgaatat tttcgttagt aacaccggcc atcaacgcat
     2581 ccagtgaata ctccttcgtt ttaacaggaa cgatggtaat tgcgccgtcc tctactgaca
     2641 gctccactac atcatcaacg tttaacgaag cggctttcac aatcgcagaa ggaagacgaa
     2701 cagacggact attaccccat tttttaatta ttacttgagt catgacatac ctctcaataa
     2761 acaaagaaaa ctaattagcg actcttcagg atgtaccagg cttctttcgc tgctgaaagc
     2821 gcccaaaccc atgccagagt cgatgcgata cacaatgcgg tcatattccc aaagtgtccc
     2881 ggcgtgtatt catggccgtg taaaacaagc ccaaccaaag aagccacact catcactatg
     2941 aaaacaatcc caaacatgat gtgaaacata aggccaaaca cacattacct ctcgtatcat
     3001 cattgttgat acatccagtg tagagccacc cctttaacgt gtcaacattg ttgatacaga
     3061 aagtgtctca caaacgatga tttttgttac accgccccca ccccatccaa aaatattaaa
     3121 atggatgttg tcagtgtaac aaaactcgtt acaatactat gtgtaacaaa aacggtgtaa
     3181 caaaagcagg gcatatgaaa ataggctacg ccagaaaatc aaccaaagag caagacacat
     3241 cccttgagta tcagatcgag gctttacaaa aagctggctg cgaaaaatgg tatgcagagc
     3301 aggtatcgag gggcggtaat aagcgcctga aaacaggtac accagagttg gaaaactgtc
     3361 tgaaagcgct tcgcgccggt gacacactgg tagtatgggc gctagaccga ttaggtggca
     3421 ctctcttgca gctcttgcag ctcatgaatg agctacgcag caaaggtatt cactttgaga
     3481 gcctcaaaga gcgcatagac acacaaagcc caatgggtga aatgtactat tccctcatcg
     3541 ctattttttc caatttcgag gtagccagaa accacgaaag gacaatggcc gggcttgcgg
     3601 ccgcaaagaa gaaaggacgc attggaggca gaaaaccaaa gctatctcca caggacatta
     3661 aagaaattaa cgcgcttgtt aaatcgccag aaattacggt aaaagagatc gcggcacgtt
     3721 ttaaggtttc acgtccaacg atttacagac atattacccc cgacagcaat actgattaac
     3781 cggcggcaaa cggtagcgaa ggagcgaaaa agtgacagac aaaccattac cagcgaagca
     3841 aataaaaacg gtaaacaccg gcatcgcaat taaagagaat cccccaaaag gggatgatat
     3901 ggcgtttact cactcgatat tgtgtcaggt tggcctaccc cgttctaagg tggatggccg
     3961 cgagtttatg aggaaatcag gcgatgcctg gctggtcgtc caagctggct ggcttgatga
     4021 agggagcggg cctgtagaac aaccgatccc ctatggttcg atgcctcgtt tagcgctcgc
     4081 atgggtatcc tcttatgctc tccgcaaaaa gacccgcgaa ataccgatcg gtaagagtgc
     4141 aaacgaattt ctgcaattaa tgggcttatc aggtggcggc agacaataca aaacgttgcg
     4201 cgaacaaatg cacgcgctgg ccgcttgccg gttgcagctc ggatttaaag gacgttcttt
     4261 caacggtcag cccgtagaac aattcgatgc ctggcagaaa gacgaccaaa gcggccagct
     4321 aagtttatgg ccgggcgtga tgaccctatc agaagggtat ttcaatgggc taattgatag
     4381 cgccgttcct ctcgataacc gcgctctgta cgcgctaaaa ggctcggcat tgtcgttaga
     4441 tgtttatacg tggctggcac atcgtttaca ccgcatcgag ggccgcccga ttaaacttca
     4501 ctggatgaat ttacgggaac agttcgccca agagtacatg ggtaaagacc ctgacaagga
     4561 tttcaaaaag aaatttcttc cagctctgcg cgaagttctg accgtatacc cacaggcgaa
     4621 ggtagaaaaa gttaccgggg gaatattact ttacccttcc ccgcccccga tcccattcaa
     4681 ggaagcaaaa aaataggccc atgcgggcct tttttatttc gggttaatgc cacacctccg
     4741 ctttgagtca agttatggat aaggtgaata agcctgtaaa aaagctgttg aaacataaag
     4801 ttatccacgt acatccgacc cccccaatga tcgtacatgc gaccccctag ctcgtacatc
     4861 cgaccccctg gctttgtaca tccgaccccc tagctcgtac atccgacccc ctttttttgt
     4921 acatccgacc cccttttttt tgtacatccg accccctaaa aacacgtaca tcagaccccc
     4981 cctaaaccta taaggtgatc tcctgtaggt ttcctgtagt ttgaccgata gcacttagcc
     5041 tataagttta tgcttttaag agatcagcct ataaaaaaag gcccgataat ttattcaata
     5101 aaatcagtgg ataaaaaata aatcgaataa ttttagttca gtgtgtacgt gccaaaatca
     5161 gaaacggcag cctttgcgct tcctcgcttt tgctgttcgt tcctcgcgtt gctgcggtac
     5221 gcctcagcaa aagactgcgg cgagcgctgc gatctcttag gtaagcgatt aggtttgatg
     5281 cagggatgca ggggggataa aaagagtgag gcccatcacc ggcaagtaat gggcctctgt
     5341 ctttcctgcg aaggagcaga ttagacgttg taagaagtat agacgaaagc agcgtgatat
     5401 tgaaagagga ttcatagcgc cttatattta acctcaatat ttaaattttg gtgaattatg
     5461 aagaaatctc tattcagtat catcctgctg gctaccacgt accaagcaag cgccgaacct
     5521 tgcatggtga tctttgaaca tggtcagtta attacgttac ctgcggctat cacacaggct
     5581 gaccaggtga aaggtatgtc aggtgaaaat cacagcaccg gcttaatcct cgcatggaaa
     5641 acagcagaac cccgcgcggt ctggatgaaa gatacgccaa ctccacttac agccgcgttc
     5701 atcggtgctg atggtgttat ccagagtatt caggatatgc agcccaacag cgacacagcg
     5761 cattcatcac tacatcccac gatagcaatc atcgagatga gaacggaaaa atttaaacag
     5821 ttcaggctat ccaaaggaga caaaatcata tccagccaat gtttccccct caaataacgc
     5881 ctcacagccc catagagctg cttcaacaag aaaaactaac tcaggtatag cccgaaccaa
     5941 gataacctct tacaaagcca tacagagcca ttcaaccaat atcacgccag taatccagtt
     6001 gtcagcgtgc ctgtaggggc gctggccgct gcgcggccat ccccccaaca cccggataaa
     6061 tcacgcccgg cccgtcgcta cgcgccagcc cgcccgctca accgggccgt tgtgacgggc
     6121 cactgttccg gcggcggtat cgtctgacag cccgccaaaa ccggctttgc agggaggggg
     6181 tgggtgcttc gccctggtcg gcgtcaccgg cgacgtaacg ccctgccccg ttcggctccg
     6241 gggaagtggg cgcgtcgccc agctcgatgc cgtaaggccc ggcaatttga ccacgccgcc
     6301 caggtggaga tcggcgcagt tgcctttcat ttgtcggcag cggtgcaaaa tttaagtatc
     6361 tttatcgtac tatataatga tactaaaagt tgacttttaa agggggaaag cctatacttt
     6421 atttatcggt tagcaatggt actgaccggc ccggaaccag cggcggcaac cgctccggga
     6481 aaaccgcgaa ggggtgaaaa atgaaattaa ccacgctgct gaaaaaacat tttgatattg
     6541 aggaaattac cgatgtggat agcaccgtaa accgggaggt gtataccatt tgggtttatg
     6601 aaaaaggcga agattgcgag ccgctattaa ttctcaaaga tgcacaggat tttatgggtg
     6661 tggatggctg gcttgtcggg aatatctatt ccacgttgca acatggctta ttgctacagc
     6721 atgaagaact gaaaacgatg atccgaaacg gcgagattaa gagccgttaa atctatacgc
     6781 ggtcggcaat agtgccgacc ggcccggaca cagcggcggc aaccgctccg ggtaaactgc
     6841 gaaggtacag aggctactat gtcagtaaaa gatttcaccc ctaccctgga aattaaattc
     6901 catcgtcgcc gctggcgtat catggtcggg cgttcaagcc tggcctcttt ccgctccgaa
     6961 caggacgcaa ttgatgctct caataaacgc cgctcattct atgagtattg ggccggttct
     7021 gccggtgtac aggcagagaa cacagagccg gtgattgtcc acgtaacata ttgagtacca
     7081 acaaagcccg gaaaccgggc ttttttttta catcacggag tacaataaaa agtaaaataa
     7141 aatgatactt ttagttgact tgtgggaggg gaaaacctat actgtattca tcggttagca
     7201 atggtgctga ccggcccgga accagcggcg gcaaccgctc cgggtaaata gcgaaggagt
     7261 atgattatgt tatctagccg ttttcgttct gcaaatatcc tgcgttctga ccgccctctt
     7321 aacgatgatc agatcatgcg ttatgccccg tctatctttg ccactgaagc gcatgaaagc
     7381 cgctccgacc gttacgccta cattgcgacc agcgaagtat taaatggtct gcgtgataac
     7441 ggttttcatc cattcatggt ttgccagact cgcgttcgta acctggataa acgcgaacac
     7501 accaagcaca tgatccgtct gcgtcatgct gggcaaatta acggcactga agcgaaggaa
     7561 atcatcctgc ttaactctca tgatggcagc agcagctatc aaatgctggc cggtgtattc
     7621 cgtttcgtct gccataacgg tttagtgtgc ggtgataccg ttgctgacat tcgtatccca
     7681 cacaaaggca acgttaccga gcgtgttatt gagggcgctt ttgaagtgct ggatcagttc
     7741 gataacgttg aaaaccatat ggatgctatg aaatctgtca cactgaatgc gctggaaaaa
     7801 cgtattttcg ctgacgcggc gttagcactg cgttatgacg agaaagacgg ccccgcacca
     7861 atcgccgcga gccagctcct tgaggcgcgt cgtttcgaag atcgcaaaga tgatttgtgg
     7921 acaacgttta accgcgtaca ggaaaacatc atccgtggtg gtttgagtgc cagaactaag
     7981 cagggcaaat caacccgtac ccgcgccgtt accggaatgg ataaggatac ttctttaaac
     8041 cgcgctttat ggattctcag cgagcgcatg aatgaactca aagccgctta atggcaaacg
     8101 ccccgcttga cctgcggggc ttttatcaac ccgaaaaagg tgattatcat ggcttcaaga
     8161 ggtgttaaca aagtaattct ggtcggcaat ttaggccaag atcctgaagt gcgttatatg
     8221 ccgaacggcg gcgcggttgc caatattacg ttagccacgt ctgaaagctg gcgtgacaag
     8281 caaaccggcg agatgaaaga gcagactgaa tggcatcgcg tggtattgtt cggcaagctg
     8341 gccgaggtcg ccggtgaata tctgcgtaaa ggttcacagg tgtacataga aggccagctc
     8401 agaacacgta aatggactga tcaagcgggc gtggagaagt acaccacaga ggttgtggtc
     8461 aacgtcggcg gtactatgca gatgttaggc gggcgcccac aaaacgcttc tcagcctggt
     8521 aatcagccgc aagccggcca gcagttcagc ggtcagccac aggccaccgg cacagcggca
     8581 gcggcgggaa acgagccgcc tccgtttgat gatgatatac cgttttgaaa agatgtaact
     8641 tactgaaata acttaaaaaa atcggccctt tacagggccg taatgaggta aaaaatggca
     8701 gtagaacgca tttcccctga agaatggcag cgtatcgcac cggcattacg cacatgcagc
     8761 caggtaacga tcgatatggc atacgccgta ctggttgacg gtcgcaaaca agtcgatgtg
     8821 gctaaagagt tcgaccggtc aaagcaaacg gtcaatgctg caatcaggcg cgttactgcg
     8881 atttttaacg aagttatccc ggaagatgag cagttagaat ttgtgcaagt ctggttgccg
     8941 ccagagctgg cgaagcaggt taaagaaatg gctaaaccat atcagaataa aaattaaaag
     9001 gagttatata aatggcaaaa gttagatttg aaaactcact taataagatg atttttgaaa
     9061 ttcgtggtta tgaatcattt tccgaaatgg aaacagcatt actagatttt tgtgatgaaa
     9121 ctatgggggc aaaccatcct gatatagtgg tggaatatcc ggtttattat aagcatttta
     9181 ttaatgataa aatttcatat gagcatatag gttatgtgaa tttaggaata gatcaggatg
     9241 atggttcttg ctatacgatt gaacatctga cattagaccg caaaacactt aaaaaccact
     9301 ggcatccttt ctatttttat aaaggcgaat gtgagtacgg atttaaaaat taacagccaa
     9361 aatcacttat ggcgttccct ccggggcggg ctttcgtgct gcgcatcgag ccaggcaagc
     9421 tgtctcgccc ctccgggctt caatccctaa cgcaatcaac tgcgctcatc ctgatcttgc
     9481 cggttttctc cggtgccatc gtgcctgtag gggcgctggc cgctgcgcgg ccaacccccc
     9541 aacacccgga taaatcacgc ccggcccgtc gctacgcgcc agcccgcccg ctcaaccggg
     9601 ccgttgtgac gggccactgt tccggcggcg gtatcgtctg acagcccgcc aaaaccggct
     9661 ttgcagggag ggggtgggcg cttcgccctg gtcggcgtca ccggcgacgt aacgccctgc
     9721 cccgttcggc tccggggaag tgggcgcgtc gcccagctcg atgccgtaag gcccggcaat
     9781 ttgaccacgc cgcccaggtg gagatcggcg cagttgcctt tcatttgtcg gcagcggtgc
     9841 aaaatttaag tacctttatc gtactatata atgatactaa aagttgactt ttaaaggggg
     9901 aaagcctata ctttatttat cggttagcaa tggtgctgac cggcccggaa ccagcggcgg
     9961 caaccgctcc gggcaacctg cgaaggaata aattatgagc gcatacgtag tatctgataa
    10021 agccatttct actatcgtta aaactctggt actgaccggc acacttcagc ctgtcgaggc
    10081 tgtttcattt ggtcagatga tgcttaatct gaatacccat tctgttaatg ttcgctatca
    10141 agaaagcagc ccggcgcacg cttttgaata cagtgaaccg gaactaaata ttaacgaccc
    10201 aaaaacacaa attcaggtga ttgtctgcat tgatgagtat gaatatcagt cctgcgagtt
    10261 tgccgaatac tatgaaacga tggtgcatac agtcctcaaa gccataaaat cagcgttaca
    10321 tgaggcttat actgaaacat tgcctaatcc cgcccggtgg aaagctaaaa aatcctatga
    10381 actgccgggc tattcagaag ctgaatggag tttatgatcg cgcgggggta agccccccgc
    10441 tctatctgta ggggcgtagc cccttttttt gcgccggtga tctctggcgt cccctgcggg
    10501 tcgggctttc gtgctacgca tcgagccagg caagctgtct cgccccttcg ggcttcaatc
    10561 cctaacgcct ccgggcttcg ccctccgcac tccgcttggc ctccgggctt tgccctccgc
    10621 gcttcgcttg gctttttcag ccggtgccaa ctccgggccg cgaggataac cggcgggagg
    10681 cgaactgtcc tccacagctc cccgttccgt tggatggtct gccgttcctc cattaccggt
    10741 attccacact agcgcggtgt ggcgtcaata gttcgcttcg ccgcttcgcg ctattgaccc
    10801 caccccttgc gccagtgtta cacaccggtt gtcggaaacg acaaaccctc caactaccct
    10861 acaggtgctt tatggccgaa attctccaac tcctcccgcc ggttatcctc gctatcgcag
    10921 ccctcatcac agccatcacc ggcctgataa aagtcctgaa aagcaagaag aagtaacgga
    10981 aaggggcggc aagccgcccc tttcccctgc tcccctggct taccctgctg tacgatccgt
    11041 ggatttcaca gcagggttat tttgggtgat cgccgtgatt ggccccgcag cggccgccgg
    11101 ttactcaggg cgttccctgc gggccgggct ttcgtgctgc gcatcgagcc aggcaagctg
    11161 tctcgcccct tcgggcttca atccctaacg tctccgggct ttgccctccg cgctccgctt
    11221 ggcccccacg ccctgcgccg gacttgcccc gcctccggcg gccccgcccc ttcggggtgg
    11281 cttcgccatc ctctgccagc aacggcgcaa tcgtgcctgt aggggcgctg gccgctgcgc
    11341 ggccatcccc ccaacacccg gataaatcac gcccggcccg tcgctacgcg ccagcccgcc
    11401 cgctcaaccg ggccgttgtg acgggccact gttccggcgg cggtatcgtc tgacagcccg
    11461 ccaaaaccgg ctttgcaggg agggggtggg tgcttcgccc tggtcggcgt caccggcgac
    11521 gtaacgccct gccccgttcg gctccgggga agtgggcgcg tcgcccagct cgatgccgta
    11581 aggcccggca atttgaccac gccgcccagg tggagatcgg cgcagttgcc tttcatttgt
    11641 cggcagcggt gcaaaattta agtatcttta tcgtactata taatgatact taaagttgac
    11701 ttttaaaggg ggaaagccta tactttattt acggttagca atggtgctga ccggcccgga
    11761 accagcggcg gcaaccgctc cgggcaacct gcgaaggaaa atgtatgaat acgactcttg
    11821 ttgttttagt cctcgccata acaggcgcgt tatatctccc ctggcgtctt gtgcgcgacg
    11881 gttacaaaga actggtgcag atgtgcctgt tttgctcctg tatcgtcacg ctggccgctg
    11941 tatatgccgt gttagtcaat ctcgatactc cacacccgtt tatgccaatg tttatcgcgg
    12001 taacggcgtt cggtgcggct ttcgtggctg actgcgcttt atctctctgg ctctatcagc
    12061 gtaaagctaa cgctaagacg gctaaccact aaccactaat taaaccgccc tgcggggcgg
    12121 tttctaacca tgatacgggg gtaaatatgg gaaatatcat cctgatggcc gagaaagcaa
    12181 aaggcgcgat tgatgaagag gctgaagtgt atgaatttga gggaatggat gatctgatcc
    12241 agtttcgtaa gaaattccct gaacagatga agtatgaata tcattacatc ctttccggcg
    12301 gcacgaaaaa ttttcggcat atcgcgcttg ttgaagccaa ccattttaag cagttcaaaa
    12361 agctggttaa tctgtaccag gatcgctaac aacgctgaac agcgcggcat tgccgcgctg
    12421 ctgcataacg tattatttca gtatcattta atgatactga aggttgactt tttggggtga
    12481 ggaatttata ctgtatttat cggttagcaa tggtgctgac cgtaagcgaa ggagcaaggt
    12541 tatgaaaaaa tttatctgca accgcaacgg caaggcggtt tttcaggaag gtgatcggga
    12601 gctggtagcc gcttaccaga tgaaatcctc tccggttccg gtgacacccc gcaagctgaa
    12661 agctgaaatg gaggctatcg ccagaacagc ccgataatca atcacccgcg ccgttggcgc
    12721 gggtatccct catagcggcg gcaaccgctt tttgcgaagg agcaacgcga tgtgtattga
    12781 tcacgtcctc acctttcccg ttcccctgga agatgccagc ttgctggcag actactgcgg
    12841 gtttgaagtg tatcccacct acagcacgaa ccgggccgat gaaacgcccc gtttttccgt
    12901 ggtggcactt catcgccaca aagccaggct ggaaacgtta gccgtggccg acacggagaa
    12961 aagcgcccat gctttccgcg atatggccga gattaccgcc gcttactact tacattttcg
    13021 gcggtaatac gatgaacgat tatctatccc tcatggaaca ggcacgacag gcagaggatt
    13081 tgcgcgactg gaaaaccgcc gcgcagctct acactcaggc aatggccgtt agtccaaaag
    13141 ccagaacgat gcgccagcgc cgcgaaaggc tgaacctaca gcagcgtatc gacttttgcg
    13201 agcgtaacgc gagaacgtaa aaactaaaaa aaaccgcctc atacggcggt ttttttctgg
    13261 ttaggttggc tggtattctg cctttccgca gtgttcgcca ccacagtaca ggatcaggcc
    13321 cggctttccc cacacgttcg tatggcattt cgggcaggtg tattttgtcc ggtttgattt
    13381 attcaccggc gtatctggcg gcgggtttac cagcgaaagg gcttgtgtga tctcatcgtc
    13441 cagctcgcca tcgtcgccgt atccctcgtc ctcataatct tcgtcatcaa catacccttt
    13501 accgtctgcg ccggttggcg ttggtggcgt tggtttgtgt ggcgggaacc gatccagcca
    13561 gcttagttta aactccgtgg taagcagctc cttgcaggcg gtatcaaacg cgccgccgtc
    13621 ctcgatgtaa tggctcatgg actctccgac gcgcttacct cctggctggc cggtgtcgga
    13681 tggtatcagg ccgatgcgct ccatcatcgc cgcccattct ttattgtggt atccacggcg
    13741 gcccggtttg ccggtgtgtt gctgcaactg gtgaaccatt tcatgcacaa gcgtggaaag
    13801 cgtgtcgcgt atcgtgcgga tcgagaaata gaccggattc atggcgattt cgtcaacaat
    13861 ttggccggtt gagcgaccaa caaagcgacc attcgagaaa tagccatacg ttcgtttttc
    13921 gcgctgaagg gtgataaggc aaggcggcag cgcactttca aacaggcgtt gattgaaaaa
    13981 ttcgtaggcg cgttgaagct cgtcgtaagt ttcctgggta gggatgatca tagcggcctc
    14041 acaatgggtt tctggattgc acaaaccatt ttattgatta tggattgtgc aatccacttt
    14101 tatttttggg cgttccctgc gggcgggctt tcgtgctgcg catcgagcca ggcaagctgt
    14161 ctcgcccctt cgggcttcaa tccctaacgc aatcgaggcc aaaaaaaacg gcccggatag
    14221 ggccgcttca ggtcaggctt tttgtcagtg aagcaggaac gttatagccg ccgcgccgag
    14281 cgctccacca acgaaggaac agacaaccgt gataatcaga atctgcctga taagcgcttg
    14341 cgaaaaccct gtttgcgccc cggccagacg ttcaaaaaat tggtcgctaa ccttctgctc
    14401 cttcttcaga gtgttgacca gctcgcgctg gcttgcgatc acttctgttt tcgccacctc
    14461 agatgatttc tttatttcat cggaggcttt atccagccgt tctactgttt cggtaacgct
    14521 tgtggttacg ctttccagtt ttgaggtgat ttctttcgca tcgcctaaaa accagtccat
    14581 caggatatct aatttatcag gttgcttttc catattattc agccgttaat atttcaaagg
    14641 tgcgatcaag attacgttta accggtaatg ctaatttcat ccagcgataa cgacgcgcag
    14701 ccgccgcacg ttcatcggca tcggtgcaag ttttagcttt tgctttaaag gtttctggat
    14761 cttctgccag cagttcttca aacgaaattt tgtgatacgc gaggtattca taaatttcac
    14821 tttcaaaaac ggcggcatct ttatgaatag aggctttttt agaatctttc gcccattcat
    14881 ataccgaagc aatatcctcc tgcggagtcg atttaatgcg gttaggcaga agaacaattt
    14941 tagatttagg aacgccagcg gcacttaacg cgagggcggt cttaatactt tcctgccatg
    15001 ctttatcatc cggggtgaca ggaatcagat atttatcaaa ttcgtcaatt gcaccctcat
    15061 attttgtcat ggtagaaaga aaccgctcaa cgttcgacgc gccaatatcc acgatggcat
    15121 tatcttcaaa tacgatttct tcaacgagtt gaccaaagtt atcgccggtt aagctcatca
    15181 cttcatcaac acccaaatcc gcagccgaca tattgattgt ctcgatggca aagaatttag
    15241 cgccagccat gcgaggatac agcaggtatg acgaaataat ggttttgccg acgctgccac
    15301 tgtaattcat tactgcaatt ttcatctgta agctccttta gaaacctttc ttattaccta
    15361 cgctaccttt gaatgaggtt gcgactttct ttgcctcatc accaattttt ttaaaatcgg
    15421 tatcggataa aaattctttg ttcccgccca tatcctcaac taatttttcg ggttctggat
    15481 tgtgcaatcc agtttccacc ggttccgctt tctcctgctc ttttggattg tgcaatccag
    15541 tatttaccgt actttctgga ttgtgcaatc cacctttttt gcgtttcaat cttgcccggt
    15601 acaaagccat atctaacgct ttcacagtca cagaaaagct gtattcacta tcaagaagtt
    15661 gagcaatttc ttgcctacta atacccttgt ctaacaaacc ttcaatctca tcaagacgcg
    15721 ctcttacagc cgcagcgaga ttgcgaggtt ccagtaaaag gtcttttttc ataccacccc
    15781 cgcttgtcga atttcactat atggcagcct gttttagctg tcaatcggtt tttgtagtat
    15841 ttcagtagta ttcctgtagt aattcagtag taacgcttgt cgatatcgct catttttgta
    15901 gtatatcagt agtatatctg tagtaatggc tatttcattt tgtagtattt tagtagtaat
    15961 tcagtagtat cggtgtagaa aactttcatg caagcatgaa agtggtgaac gtgaacgctt
    16021 tttgagcgtt ttatgcgtgt gtaagagctg tttctccctt aaaaaaggcg tgttatggta
    16081 tcccttaatg gctatacacc atacgcaatt acgattttca tcgtaaatgc ttgaaaacaa
    16141 aacaaattat tctaatttgt gttttcatat gtaatacact gcgtattaca tatgtgtgtc
    16201 taagccgcat atatgctaga tttgtatgta ttacatcggt attacacaaa ggaaacctat
    16261 gccatctatc agtttaagag tgaccgaagc gcaaaaaatc gaccttgaat accgttcgga
    16321 gggcaacgta tcggagtaca tcaagcaaca gctttttgga caaaaagaga gtgccagtga
    16381 agtgatcgcc cggcttgata aattgctgga acgttcatct tcagagcagc aagccgccgg
    16441 acgagatcga tacatcgagg caatgttgat tgaggtgtta ttgatacttc gccaggtgag
    16501 taaagcagat gcgcagaaag ttgccggtgg tgaaattcgc cggttagggc tggagccgtg
    16561 gtcagcagag ctggcccagg agagagagga acagagtcat gaatgaagcg gataagggca
    16621 aaatttgcct gggggcattg ctttttaccc cccttgttac gtggtttatc gcggcgaaag
    16681 ccttgtatgg ttttacgcca aaagatgcgc gtgaacgtct ggttttactt attcagcaga
    16741 cttttgattt atggccttta tggggtgctt tattagtcgg tgaaattatt gccatcatag
    16801 gcttaatcat atttttcaaa tttaatcacc agatatttaa aggtgcacca tttaagaaaa
    16861 tatatcgtgg tacgcggctt gtttctcagc gggctttagc atccataact aaagaaagag
    16921 agccacaaat aacaatagcc gatattccaa tccctacaaa agcggaaggt acacatattt
    16981 caatagccgg ggctaccggt gttggtaaat caacaatctt tgctgaaatg atgaaatgtt
    17041 gcttgatgcg tggaaaaatg caacgtgttg cctcaaataa agaccgcatg ataatcctcg
    17101 atccagatgg cgatttcctt tctcgttttt ataaaaaagg cgataagatt ttaaatccct
    17161 atgatgccag aacagaaggg tgggtatttt tcaacgaaat aaaaagtgat tttgactttg
    17221 agcgattcgg tgtttctatc gtacctacag cgaaggattc gcaaacagaa gaatggaatg
    17281 gatacggtcg tcttttattt accgaggtat cccgtaaagt atttaacaca agtcgaaacc
    17341 caacgatgga agaagtattc tactggacaa atgaagtacc actggaagaa cttgaggaat
    17401 atgtcagagg cactaaagca caggctttat ttgctggtag cggtcgggcc gttggtagtg
    17461 ctcgctttgt gcttagtaat aaacttgccg ctcacctgaa aatgccagca gggaacttct
    17521 caattcgtga atggctggaa gatccgaaag gcgggaatct gtatatcacc tggacagatg
    17581 aaatgcgtga agccttgaaa tcgcttattt catgctggac agatagcata ttttctattg
    17641 tgttaggtat gagcaagagc aactcccgta aaatctggac gtttatagat gaactggaat
    17701 cccttgatta cctgccatca ttacgtgatg cgttaacaaa aggtcgtaaa aaaggactac
    17761 gggttgtcac cggctatcag tcgtattcgc aggtcattag catctatggc agtgatgtag
    17821 ctgaaaccct tctgtctaac catcgtacat cggttgttat ggccgctggt cgcctgggtg
    17881 aaagaaccct cgaatttgtc tctaaatcat tgggggagat tgagggcgaa agagaaaaaa
    17941 cggggattag ccgccgcttt ggtcagttgg gaaccaaaaa taccaatgat gatcacaagc
    18001 gcgagcgggc tgtaacgcca accgaaatcg ccacgctgga tgatctgacc ggctacatcg
    18061 cgtttccagg aaatctgcca gtggctaaat ttgagactca tcacgttaac tacactcgtt
    18121 ctaaccctgt accgggcgtg gtcttacgtg aaagcaccgc atttgcagga atgtaaatat
    18181 gctgaacatt acgacgatta cccgccagcg tgtcgataag gttgttgatt actatgctga
    18241 tggcgcagac gattactatg cgaaagacgg taacgcgatg cagtggcaag gcgctggcgc
    18301 ggaagaactg ggcttaaccg gcgaggtaga acaaaaacgg tttgcgaaat tgcttgatgg
    18361 caaagtaacg gatgacattt ccgtgatgcg taaaacggcc aacagcgaaa gcaaagaacg
    18421 attgggatac gatcttacgt tttccgcgcc gaaaggcgtt acgcttcagg cactggtcaa
    18481 cggtgataaa cgtatcattg aagcgcacga tcgggcggtc actaaagcca tcgaggaagc
    18541 ggaacggctg gcactaggcc gctttaccgt caataaaaaa acgcacgttg aaaacacgaa
    18601 taagctgatt gtcggcaaat ttcgacacga aacgtcacgc gagctagacc cggatttgca
    18661 cacccatgcc tttgtaatga acctcaccaa aaccagcgat ggtaaatggc gttcgctcac
    18721 gaatgacggc attgtgaact cgcttacgtt attgggtaac gtttacaaat ccgagctggc
    18781 aagagagctg gagctggccg gttacaacct gcgttatgat cggaatggta cgtttgattt
    18841 ggcgcatttc agccaggagc agatagccca attcagccag cgcagccagc agattgaggc
    18901 cgagttagca aagaatggtt taagccgtga aacggccagc catgagcaaa aaaatgcggc
    18961 cgcgctgaaa actcgtaaaa gcaaaggcga agtagatcgc gatgtactct atgagggctg
    19021 gaaaaaccgc gccaaagagc ttcagattga ttttgatagc cgggagtgga aaggtgcggc
    19081 caacgacgat atagcgcgga atacgcagcc agcggcgctg gaaaagccta tcgagtacca
    19141 ggctgacaag gtaatgacct ttgccattcg ttcacttacc gaacgtcaat cggtgattac
    19201 cgagcgggag ctgatggacg tggcgatgaa gcatggctat ggccgcttaa ccctcgatga
    19261 tattcgggct gcgagagaac gggccataac ctccggcaac ctcatcaaag aagaagcaac
    19321 atacgcagcg gccacgacca ataaaaaaca gcagaacgta gcaatgaccc gcgaacaatg
    19381 ggtaacggaa ctggtgaaag ctggccgcaa taaagatgag gcccgtaaac tcgtagataa
    19441 gggcatcaca aacgggcggc tggtacagca aaagccgcgt tttaccacac agttagctca
    19501 acaacgcgaa cgcaacatac tgaagatgga gcgggaaggg cgcggaaaga tccagacccc
    19561 atacactcgc gagtttagcg agggctggtt agccagcaga acgttaaaac ccgaacagtt
    19621 aaaagcggtt atgggaatta ttcatacccc caaccagttt atttctgtac atgggtttgc
    19681 cggtactggc aaaagttata tgaccaaatc ggcagcggat tttctaaaag agcagggcgt
    19741 acacgtcacc agtcttgccc cctacggtag ccaggttaaa gcgcttcagg ctgaagggct
    19801 ggaatctcgc acactccagt ctttcctccg ggcctcagat aaaaaaattg gccccggctc
    19861 agtagttttt attgacgaag caggggtaat cccggcccgc cagatggagg aaaccatgcg
    19921 cgtgatccgc gatgcgggcg cacgagcggt attcctgggc gataccaaac agactaaagc
    19981 cgtcgaagcg ggtaaacctt tcgagcagct tattaaagcc ggtatggaaa cggcctatat
    20041 gaaagatatt cagcgccaga aagacccgga attgctgaaa gccgcgctaa atgcagcaga
    20101 agggaaaata aaggcgtcgt tgacccacgt aaccagcatc gtagaggaaa aaaatcatga
    20161 tcagcgctac cgccgcattg tcggggatta cgtggcaatg ccgccgacag accgggctaa
    20221 cgccctgatc atcaccggca cgaacgacag ccggaaaaaa attaatgagt acattcaggc
    20281 cgagctgggg ctaaaaggcc agggtatcaa ttacccgctt ctgaaccgtc ttgataccac
    20341 tcaggcccag cggcagcaca gcaaatatta cgaaaaagga gccgtgatta tcccggaacg
    20401 ggattacagc aacgggctga aacgcggggc cgtttatacc gttctcgata ccggccccgg
    20461 caataaactt acggtaagcg acggcagcgg cgacacaatc gcattttcac cggcgcgatt
    20521 ttccaaatta tccgtgtact cagttgaaaa aacggagctg gccgtaggcg accaggtaag
    20581 gatcacccgt aacgatgcgc atctggattt agcgaacggc gaccgtttta aggtaaaagg
    20641 cgtccagagt ggtgaggtac tgctggagaa cgagaaaggg cgactggtaa aaattgatgc
    20701 caacaaacct atgtatttag ggctggctta cgcttcgacc gttcatagcg ctcaggggct
    20761 aacctgcgac aaggttttta tcaatatgga tacccgatca ctcacaacgg ccaaagatgt
    20821 ttattacgtg gctgtcaccc gcgctaaaca cgaagccgtc atttacacag acgatgagaa
    20881 gaagctggat aaagcggcca gccgtgaagg gttcaaaacc gccgcgcttg agctggaaca
    20941 actcaaacgc tatgccaaag agcagcagca tgatgcccgc gaaaaaggcc gagacaaaac
    21001 cgagccgacg caggaaaaag acaatgccaa gcgtaaaggc aaaggtcatg acaaaaataa
    21061 cgacgagaga gcatttacct cgctctaact gactaatccc ccgaacagca ccggcagaac
    21121 aaacccaccc cggattattg ggagccggta ttgtccgggg ggcattatcg tagcatttgt
    21181 gttgcataca gcccgtaaaa actcattcag tgaacttgaa caaccgtttg tgtggccacg
    21241 tcgccggcag cagctggcca acgccggcgc cgaaaacgcg aagtgaataa aaacataaag
    21301 ttatgagtgg aggggataag aaaagggcat tcaaaaattt cgatgccctt ttgtggaagc
    21361 ctgcgctatc gcggggaaaa accttcttcg gccattgccc tctgtaattt atcgacggca
    21421 tccttgatga catacgcctc gtcatcatca acggcacacc cccgtaaatc actccacgtc
    21481 aaccgcttaa caaactgcgc cagcgccagc gcttctttgt catccagctc taaagacaac
    21541 gtgttgatac cttgacccat aacccacctc gttagtctgt aaaacggtac gttttcttac
    21601 cgccctgcgt ttcatgaatc agcttaaacc cgcttccgtc cagcagtctc atcatttttg
    21661 tggccggggg gagacaggag gcttccagcc ggtgtccggg cgtggcttcg atgattgagg
    21721 caagcatact cttgccaatg cctttaccct gctcatcagc cgcaacagcg aacatacgga
    21781 tctcaagatg agatgcccgg cccgcaaaat cattgcggcc ggttaacagc gaaaatccca
    21841 ccggctgccg cgttaacttc tcggcaaaga caaagagata atccccaacg gggatattat
    21901 ctctgtattg ttgggtgaca ctttgtagct cagatctcac tgtttcaaga ccgttgccct
    21961 cggcatagaa aaatgcgcca gatgttatcc catcgcgcac caggttcatg atgaaaggaa
    22021 tatcgccgct gtttcctaat cgcatatcaa gcatatcgtt ctccattcaa taagacgggg
    22081 taaaatcccg gccctgattc cagcgtgatt gccagtgctt caggtactgc ccggccagtt
    22141 ctggcgcgtc ccacatcaca atcgcgtttt cagagttata tttctcggca ctggccgtgt
    22201 agttaaaact gccagtttcc acatgacggc catcaacgat gatcaccttg tcatgctgga
    22261 ttttgtagtt actgttaacc cgaagcggaa tacccgcgtt cgtaacaaga ttcattgccg
    22321 cacgactagc gcgacccgta ttgccattct catcaacgac aattttcacg tctacaccgc
    22381 ggcgtttggc cgcaatcagc gctttcataa tatcgggggc ggtaaagctg taggccatca
    22441 ttctgatgtt atcttttgcc gagccgatta cgttgataac gagctgctgc gcggttcctt
    22501 ccggcgagaa acctacttgc gccgatgcag caaaaagaga cggggccgaa gccccgtaaa
    22561 gtacggcggc aaccagtgcc gctttaaaga gaaagttgcg cttttttaaa cgctggatca
    22621 taaagaacct caacaatctg gcggtgttcc atgtacacca cgacatctat cgtggtgtac
    22681 agcgttttca taatcacgtc ataatccagc gtctggccga cgccggtttg cttgataagc
    22741 agcgcaatac ggctgaaagt ggattttgca tcgtcggcgt gggtggatgt gatcccgccc
    22801 ggatgcccgg tattcagcga gtttatgtaa tcccatgctg catcatccct aagctctgtc
    22861 atcatgatac ggtcgggaga aagacgcata cacgctttca ggctgaccgc cggggccaga
    22921 cggccatcct gatcgccgta cagcatataa accatttcgt gatgcagctc agacccaatt
    22981 tcgtgaacgt cctcggaaac tacaatgcgc tcggtataag gcacttcccg aagcagggag
    23041 cgggtaaacg ttgatttacc cgaaccggta gaccctgata caacgatgtt cagcttgttg
    23101 cgtaccgcaa ttttaaaaaa cgaaacgagg tcgtctttat cgagcaactc cagcaattct
    23161 tgctcgaatg gctgaagggc tacttcaccc tgaacctttc gtttccagcc agtgaaacgg
    23221 ccctcatcgc ggagttgttc aagagactta tcaacgggca gatgcttacg aaacgcgatg
    23281 gctactgttc cctccagtac ggcagggggt aagcaaataa ttccccttga gccgtccggc
    23341 agaagcacgt tattaacgga ctctttcggt agtccgttat aaccacaaag cgtgtcggtt
    23401 aagcgtgata tatattcagc cgtaattttc tcatcgtcgt ggaaaatacg acctttcgac
    23461 gtttcacaga caacctgctg atacttgtta attctcagct cccacacatc gggatcactc
    23521 aggtagcgct gaagtggttc gagcgtcagc cataccccct gctcacggcg ggggcgttgg
    23581 cgaatatctt gaatattact ggttaactca gtcattcatt tctaacccat atacagtgct
    23641 gaaatccaga tcgcgggcta cgtagatgga tacggcatcg ccctgcgcag cgtagagcgt
    23701 tggcgggata ttaatcttcg cccgtaatgc ttcagtagca agctggtcgg tactgttttc
    23761 cgttgtgtta aacgtcacat cggagttgtt gttgttaatc gacgcgatac cggctttcat
    23821 gccatcagtc agcacagaga agaaaatggc atcgcccatt cgttcccaaa agtgagagtt
    23881 gacctgacca ggtacgcccg cactacctaa agcgttagtg ccgctgctgt cgaggttaac
    23941 aatggtctga tccgggttac gcaagcgtga ccagagaacg aacacacgat tctggccgtt
    24001 ctggataccg ctattctgct cgccagtgac cacagcccct ttatcaatca gttttacagt
    24061 accgtcagca gagtatacat cacggcttac agtacatttc acctggcccg gaacggtggt
    24121 atccagttcc gtatttgtac cgcaaggaat cattgttccc tgcgcaatgg tcagatttgg
    24181 atgcggcatt acagatgcct gggcgacagc catacgcgcc ggtttcattc ggctcgccag
    24241 tgcctggctg tttgagctgg tttctgtatt gtcctgacct gaccggcgct cctgttcttt
    24301 cgcagtcgta gcctgtttct caggatcgac accgttgaag gacaccccta accgggcgcg
    24361 cataattttt tcttccggtg ataattcctt tttctggtta ttcaatgttt gacgcgcaac
    24421 ctgctgctgc gactcctgcg tcgtctgcaa cggaggaacg acgctatttt ttggcgtgtt
    24481 gtcgatagtc gggggcacat catcaaaaga ctggcgcggt aaagagctgg tcataacctg
    24541 ctctttatcc tttttgggat cgtcggccag cttcttaacg tgcatctggt tatagatgaa
    24601 ccatcccacc attgccgaac ctatcaacac catcagcccg acaaaggccc gcgcaccggc
    24661 aatacgtttt ttgggggaac cgctgaagcc ctgacgctca tcctcgatgc cggtatcttc
    24721 ttcatcgaga taatctaagt tttcctctct gtcttttctc atgatttatc cccctcaccc
    24781 tttttcacgc gcttaacatc tggtgacgta gtgccggtac tgatcgcccc tagatttggg
    24841 ttgtaattca tattcagaac acccacgact ttatttccgt aacgaatacg ccattctttt
    24901 gctaccgttt cagccacaac aatgttgtta tattccccgg ctggatgctg gttaacggtg
    24961 tgttctttac catcgtcacc gatatagaac acaacgggta aatcagcatt cgcagggaat
    25021 ttgaagtatg tgaagcggaa attatcccaa acgttcaccg gggccactgt ccgatattca
    25081 tcttcatctg acattttata aagcaggttg taaggcccgt tctgaaggtc tttcaacgcc
    25141 tttttcacgc ggttactttc cagcgtttta ttagcctttt tcatatcctc aaatggatag
    25201 agataagtaa gctggagagt ggcatttttc atactccacg gcgtcttgat aaagttggct
    25261 ttaacgttac cctgggcgtc tttcgtggtg tagtcaccta catagtgcaa cacaatgtta
    25321 taagtgcgtt tgttcgtgac cagaacaagg ttagtatcgc tgggaacttc ccctttcggc
    25381 ttgataaaca tgttgttttc aacatgggta aacgcataac tattgctgtc accaaaagcg
    25441 tgtgtcacat agctttcatc aggcgcaacc acgatatgag tggcaacacc ggcaacggaa
    25501 tcaatacgca ccacgtcaac cgggttatac accacggatt taatacggta gtcgtatggg
    25561 ctaccttttc ccacttccag cgccagcgcc tgaccagaga ggccgaggct gaccagaatt
    25621 aaaccggcga ttaattgttt tttcatgatc agcctcccac tgcgccaacg ttctcagcgt
    25681 tcttacggta actcgttaca cggaagccaa gcgggttgat atagcgctgc tgcgcggtca
    25741 ttggtaacgc tttgtactca tacgcaatcg tggcaatcca gtagcgcggc ggctcagggt
    25801 tcggacgctg gcgaatgcgc tgctgcgtgg taaagcgaac ggtggcaaca ccgtgttcgc
    25861 ggtcggtgat caccgagttg atatgaacgg tcgttctttc agagtcaccc aaccgcttat
    25921 caatgcggtt cgggcctttg aacatggaga gatattcatc ggctacatca ccggaagcca
    25981 tcaggctcat ggcgtcataa tcaacctgaa tactgttgta gtcgtaggac tcacggtgaa
    26041 taacaaattg tgaaatccaa tactggtcta tcacgtcgcc ataagtcgct tcatcgcggg
    26101 taattttcac ctgcgatact tcgcctgtat ctttattaat cgtggtgagg tatgccggta
    26161 acggctgcgc aaaacggata atgacgccga ccgcagtaat catgctcaca acggcaaaga
    26221 cgaaaaaggc cgtggcaatt ttccaggcgc gagagcggga attaaggatc tcattaacca
    26281 tatcgcgctc aagcccttga gtttcctcat ggtacttatt cagatcttcc cgcgatatcg
    26341 cttgatgttt gtttttcttc agcattgtta ctctccgttg ttagcggcat ctgcacgata
    26401 cagctcggcg ggatacgtct tattaacatc aaccagcttt ttcatgttgg gttctggcgg
    26461 cggcgtcggg ctactggaac aggcggccaa cagcagcccg ataagtgcca ttgccgtgta
    26521 tttgaagaaa tgcaccatct ttgtcactcc ctttgagtca atcacaaaaa aataataagt
    26581 catcaaaatt acaatttcaa tgacttatta aaaattaatt tctactccac ttttagcgcc
    26641 gtgaaccctt tgcgaaaccc tgcatttggg aacccgcagc ggtggccgct cccgcgccgg
    26701 ggccaccggc agccgtagcc gccgcagtct tagcgccagt accgcccccg gcacctgtag
    26761 cgcctttttg accaccaggc tgattgccac caccgccagc tccacggcca ccgccaccgg
    26821 cgttaggtat cagctcctga atacccgcag agataccatc cccccaacgg gaggctatct
    26881 caggaatgcg catcaggatc aggtaagaaa caatcattat cagcagacaa gcaaaaaccg
    26941 gccctaacag gctactgtca gccccggaag cggtgacggc atcgagcgct ttttgataga
    27001 acttaatcat caggccaaac agcatcgaca gcagaattgt taccagccca tagttaacca
    27061 tcgagccaag ccatttattg aacatgccat acagcggctt gaacaacagg cagaagatga
    27121 agataggccc aaagcacaca gtgattgcca gcagtaactt cgccatcagt accagcccgg
    27181 ccccgatacc acagatcaac accgtggaaa taatgaccgc gcaagccagg aataatgacg
    27241 ccaacccgga accgctataa atatcggcgt tatcaaacgc agtacgcgca acctgcatcc
    27301 cgtcatggat ggccttgtcg atgttgttac ccattgcatc ttcagtgcca ccggacgaac
    27361 cgttaataac cagtaccgag gaaaactcat ccgggatctt catggccgca tcagccagtt
    27421 gggtttgata cagcccgccc gcgctggcaa agctgattat catcgaccac acaatgaatt
    27481 tttttatcaa cgatgagagc ggctcaccgt gtggccgggc taatgtaaac agcccgtcca
    27541 gggtgagttt gcaaatcaac gccaacccaa ttaccggtga aatggtggat ataacaactc
    27601 cgagattaga ggccacaaat tcctttgtga ccgtatccat gtaattgaaa atatccgttg
    27661 taaatgaaaa ggccatgctt tccccctacc ctaatcaggg cgtgactttt ggcattgaga
    27721 ggccatcatc acttttgcca aacactttca aatctttctc tgcttccagc gcgttctgac
    27781 aatccggctt cactttgagc gccgcatcat cacgacattt ttccaccatc gctttacgct
    27841 cctgatcgtg ttccttgtac caggaaacag gatgttcaga atcacaaccg gccagcagga
    27901 aaagagctaa aggtaaaatc agttttttca tggtttattc cgttacgtta ggcatggaaa
    27961 gatcttcacc gtcagcgccg aggacaaagc gcttctgggc gtattgtttt tgttcctgca
    28021 aaatcttgtc ttgcgaggtt tgcagcatcg ccatcagttg cagcttcgtt tgctccccct
    28081 gaatagcacc ttgcgcggtc tggatacgcg cctgaaggtc agcgatttgt ttcatgtccg
    28141 tggttgagtc aatctgattg gtcagctcct gaatgttatt cagctccgcc atttcattgt
    28201 tgtaagcgtt ctgcgccatc acacggtcat acgccccttt ctccgccaac ttctgcttaa
    28261 tgaaactcat cgcttcggaa cggttcatac cgtcaatctg cgactgaaat tccttcagca
    28321 tggattgagc agatgaagtg atacctgaac tgctgttcat ggcgtcgctg tatacctgcg
    28381 tccagtcaga cggtaaattg cctttcagcg tactaatatc gccattcagc aaattaccga
    28441 taccagagct gccggttaag gaagccagcg tttgctcctg cttcagcaat tgagatttga
    28501 gctgttcaat ttgctgcgtc attaatgtga cctgctgaag cgcctgggcc agatttgcag
    28561 gatcgttaac taccagttgc gcattaactg caaccggcga aatagccaga gctaaaacaa
    28621 ggcttgcaag tgtctttttc atgtcgttac ctcacgctgc gagtacagat ttccagtagg
    28681 gttcgagcca tttatcaggg ttatcatccc caatttgctc aataattttc gccagtgttt
    28741 cggcgttttg ggtttcccct gacaggatgc taaggatctt atccagcttc ctgaagttct
    28801 ccggcgtctt aacgcgcggg gccaggttga attgcgccat cgcgctttgg ctgccttgtt
    28861 tcaccaggaa ctggcgggag ttctcaggaa tagacagcag cgcgtcaaac tctgccggtg
    28921 tcaatttgag gcgagtaacg tagtcctcat aatccgcatc cgggttttcc agaagaattt
    28981 tcgttacgca ctgctgcatg atggttttac cgatggagct atccagcgca tcgttcggtt
    29041 cttgcgtgga gaatacgtaa accgcatctt tcttacggtc tgttttaagg ccacgtttga
    29101 cttccacact cataattgga tcgtcaaggt actggtgaaa ctcatcgaat acctgaatca
    29161 cccggcgacg gccatcaata gactgacgca cacggaaatt gagatacatc aggagcggag
    29221 tacgtgttgc aggagggatc tcattatctg caacaatgaa atctgtcagg tcaaagccgt
    29281 agatatcgta ttcaccgaga ttcagtgaat ccgagtcgtt atcgaacaac caaccgtttt
    29341 catggccgcg agtccacgga acaagcaatg acgccagtgt ttgtttactg cccgctgttt
    29401 gatatggatt cggcatctgc tgaattaaca tatccacggt gcgtaaatct gtcggaatga
    29461 gggagttctc atccatcaaa gcatcgacgg cgcggttaag gtcattaacc tgatcagcat
    29521 cgagtacgct gcctttggta atttcagcgc acaggcgcat caggcgctta caaaatgcaa
    29581 tgttaacttt ggtgccaggc atctgtagag gtgcccaccc ggaagcaacg ccctctttca
    29641 gaaccgtgta gtgaccgtgt aacgctttaa tcatcggcat catgccctga tcgcggtcaa
    29701 aggcaaaaat gcgcgggtta tacttcatcg cttgcgtcag caggaagtta agcagcgtag
    29761 ttttacctgc ccccgaacgc ccaagcatta acgtatgcgc cagcggtcgc ttaccgaaag
    29821 acatttcatc ttctggcgtt acatgcaagt tcatatacag cggcgtattt gccaccgttt
    29881 tgaacagcgt cagcgctggc ccccacgggt tattatttgc tttcccggac ataaaattat
    29941 ggaaggggga gaagcaaaga aaattccagc tattcagcgg gcaaggtcgt ggcgacaggt
    30001 gttgattagc cggaagtttt gcataatatg ccgcttctga agctaaccct acctggctgg
    30061 caatcagccc gcagttgttc agcatatttc tggcaacacc ggcgtttgaa cgggcctctt
    30121 taagcgtctt gccaaaggtg tgtaccgtca ggtgatgcca gcccatgata aaggtacgtg
    30181 aaacaacgcc atcaacggcg tcattcatct ggtttatctg cgtaaaggat ggatcgcctg
    30241 tttccagtag tgctttttgc tggttagtga ggtaagttct tgccgcttgc tgggacatac
    30301 agcaatacgt ttgcgttaac acaaactcaa acggtgcttc catcaggata ttgatctgac
    30361 ccggctcagt atcggaatcg taatcccgga tttcaacggc agacgtatag cggcgttcgt
    30421 cggtaccgcg aatctcgata ccatcccccc aaaatgaaga agttgggcgg gtagtagcca
    30481 aatattcacg gattcggcca cgacaaaccg gcactggcgc ccactcacca ttgacgaggt
    30541 aatagagcca ctccagcgct ttagaatagg tgtgttgagt gccactaaag acttctttat
    30601 cttcctgaac gtcctgatat tccacatcgg ccagcaaatc ttcacgcaga tcgataatat
    30661 cctcatcatc ttctgcatca tcttttttct gttcaggaat gacgttcccc cgtttgtcac
    30721 ggaagtagta gcctaagcgg tcaatgccat aaggacgcag ggagttcatg acctgctcac
    30781 aaatatcttc cagcgcacta atagcatcag cctgcatttc gcgcagctcg ttggccgatg
    30841 gcttatcaaa ctttgccagc atcttttgag cggcatcccc cacagggtta taaacaaccg
    30901 tcaggtacag gtcatttacc atcatggggg tatcatcaaa ctgatcacga taatattcat
    30961 tcaccatacg cgggaacgtc atattgtaat gcgtgtcagg ataattatta atttcgcggt
    31021 ggtgtaagtg agtccataat tttacatgct cactaccaat cccttttaaa agctgattca
    31081 aatcagcgtg ccacttaatt agctcggcat cagaagcaca gtcatgggtt cgcccacgga
    31141 ttttaaatat agaaaggtac tccccattta ccgtactgat aacatggggc gtcacatggt
    31201 aggagtaagg aatatatttc aaagaggaag gttctttttt gaaatctttc accccccttt
    31261 ttttcatttc cgctacggcc attatttttt atctccataa tcagtaggtg agtaactaga
    31321 gccgccccac agtttagtaa acggggagtt aatcatattt agccctttcg tcatgatagc
    31381 cagccatata atacgaaaca tacggtcatc atgtttgctg atacaaaact caataaacca
    31441 cagcaagcca aaaagaacta cggcccaaag gtggatcgtc ataaataacg tcgcacaaaa
    31501 tatgaaggtg gcaataatta ctgttcgcgg aacaccggca aatgtcggca gtcgcgtagc
    31561 gcctttaaat aaaggatatt taccggataa attcatcgtt tcctcccgtc atatacgggc
    31621 cgggggatac gcccccggcg aaccttctta actcagcccg aaaaggccaa caaggtaaga
    31681 cgcggaaccg acaacaatca gaccgataac aatacgaacc gcccagctcg ccgggagcat
    31741 atgagcgatc catgcgatag cactcacgat gataagaacg gtggccgcaa tcggaatcca
    31801 cgtcatcatc caagactgaa cagaactcag agatgattca ccggtatctg caccggcggc
    31861 cagcgccagc tcaggaacac acagagcaac catcatgaac attgccaccg ggaaaaaacg
    31921 tttaatcgct gtattcatct ttgtctaaca ctccatcatc aggaaaaata aaatcaggct
    31981 catccaggaa aagccccact tctcttgcat taaaatcgtc tctcagcatg tcggccagta
    32041 tccgcagatc ggcccacgtt ctaaccccct ctccagactg aactctcagg gagcaagtga
    32101 ttacgccccc ctcactattt ttcacatcaa aaagcggtat ccatttcttt ttatccatcc
    32161 gttgaatagc gatccgaaca actttgtttt gattgaatac ttcttctaac tgcttcaaca
    32221 agatgagata aacgcgcatc taaaatgact cctgttactc cctacttctg ccggaaagcg
    32281 tcaccatcag atgcaccaaa agcgtcttta tccgagccac taaaggcatc agtcataccg
    32341 gcgtcttttg gttgttcttc accatcagca gcatctaccg gcacagcatc tatagctgta
    32401 tcaacacttt ccacatcagt ggagccatca tcggctttca gagcagggat tacgggtttt
    32461 gattgcccaa tttgagcaac cacctttgat acataaccgt ttgtaaatcc ggcggttagt
    32521 gaacccgtat tataacaaga taaagcgtgt ctcaaagctg actgccccgg cgcatactct
    32581 tttgttgcga gctggtagca gtaattaaga acgtattgag cagcttcgag atttttacaa
    32641 ccattaaaca aatctgcggc agaaacattc aattttggga aattcgcaga gttgatttgc
    32701 cctaaaccta cgtcaaagtt ataaccatga gccgttagcc attttgtcgt tttaacagct
    32761 tcatccaggg agcgcggttg tctggctaac ggtgattttg ttcctttagg aacgttgacg
    32821 tttatcgcgt atgggttgtt gcgcgattca tggctaacta ggtacccaat agttaaagga
    32881 tgcacttctg gcgcacaacg ttttgccaga gccataaccg gcgctgacag ttccgcattt
    32941 gcgggtgtgg catccaaaag gccagcaatc aacagtggga aaagggcaac aacctgcttc
    33001 atctcttttt tccttgaaat tgcaatttca atgattaact agtatagtaa aggtaaattc
    33061 gaacatgtca agacaagatg aaaagaagca caaaacagag caggagcgcg aaacatcagt
    33121 gcctaaaaga aaaatcatat tcgtaccctt gcagtcagaa aagagtccca aaggggagcc
    33181 tcgctaccga gatccagcca atccatttaa cacatgggtt ggaaggggaa agcgccctga
    33241 ctggctaaaa aaatatcttg acgaaggccg cgagctttcg gagtttgaga tcgtagaata
    33301 aggagctgac ggatgagtaa aattattgat tttcctaccg gaaaaaagag ggagaaaagg
    33361 gccaaagcaa tccgagcgcg tcgtaataaa gaagcgcagc agcggagcga aataaccccc
    33421 tcccaatttt ggtgggttaa gaaaataaag ttactcaccg tgattaaaca gtcggcacgt
    33481 tttctcttaa tatatttagg gttattaact gccgacatcc ttatctatct cctttttatt
    33541 ctaggaaaac tcctaaaatt ctttacgatg attggtatca tcgcggtgct tctgtttctg
    33601 gtcgtggaat ataggaatga ttggcattat acgcaatctg cattccatgc cgcagcaata
    33661 tttgttggcc tgatcgccct gcgcatcggg catgacaaac tactgaatgg cctaataagg
    33721 cttaaatcct atctatccag aaaagggtga aataatgcgg aagataataa ttgcatccgt
    33781 ggtgatactg gcatcgtctt attccgttgc cagcttcgca aaagatccat gcaagacatt
    33841 agcttgcatg gctgcaaaat caggtggcga gtttgggagt gttggcgata gtgattgctc
    33901 tggtgctatc gctgacttct tcaatatcgt gaagaaaaac aagcatggct ttcttcctaa
    33961 tcacacagca gatgcgcgta aagaatttct tatggaatgt tcaggcgcgg agcagaatac
    34021 cgccgctgtt agcagggtta tttcaatgtt t
//
