LOCUS       NZ_CP054281            12137 bp    DNA     circular CON 12-MAY-2024
DEFINITION  Citrobacter freundii strain IDR1900015725-01-02 plasmid p4C12,
            complete sequence.
ACCESSION   NZ_CP054281
VERSION     NZ_CP054281.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15086848
            Assembly: GCF_014169975.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter freundii
  ORGANISM  Citrobacter freundii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1  (bases 1 to 12137)
  AUTHORS   Prussing,C., Snavely,E., Singh,N., Lapierre,P.,
            Lasek-Nesselquist,E., Mitchell,K., Haas,W., Owsiak,R., Nazarian,E.
            and Musser,K.
  TITLE     Nanopore MinION sequencing reveals possible transfer of blaKPC-2
            plasmids across bacterial species in two healthcare facilities
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12137)
  AUTHORS   Prussing,C., Snavely,E., Singh,N., Lapierre,P.,
            Lasek-Nesselquist,E., Mitchell,K., Haas,W., Owsiak,R., Nazarian,E.
            and Musser,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUN-2020) Division of Infectious Diseases, Wadsworth
            Center, New York State Department of Health, 120 New Scotland Ave,
            Albany, NY 12208, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 15-OCT-2019
            Assembly Method                   :: Flye v. 2.6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 159x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014169975.1-RS_2024_05_12
            Annotation Date                   :: 05/12/2024 03:40:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,095
            CDSs (total)                      :: 4,976
            Genes (coding)                    :: 4,823
            CDSs (with protein)               :: 4,823
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 69 of 153
            Pseudo Genes (incomplete)         :: 73 of 153
            Pseudo Genes (internal stop)      :: 27 of 153
            Pseudo Genes (multiple problems)  :: 16 of 153
            Pseudo Genes (short protein)      :: 1 of 153
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP054281.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12137
                     /organism="Citrobacter freundii"
                     /mol_type="genomic DNA"
                     /strain="IDR1900015725-01-02"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:546"
                     /plasmid="p4C12"
                     /geo_loc_name="USA:Maine"
                     /lat_lon="45 N 69 W"
                     /collection_date="2019-01"
                     /collected_by="Maine Center for Disease Control and
                     Prevention"
     gene            complement(join(9170..12137,1..33))
                     /gene="tnpA"
                     /locus_tag="HS092_RS25310"
                     /old_locus_tag="HS092_25320"
                     /pseudo=""
     CDS             complement(join(9170..12137,1..33))
                     /gene="tnpA"
                     /locus_tag="HS092_RS25310"
                     /old_locus_tag="HS092_25320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152392.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn4401 family transposase"
                     /name="tnpA"
     gene            143..1858
                     /gene="tnpR"
                     /locus_tag="HS092_RS25315"
                     /old_locus_tag="HS092_25325"
     CDS             143..1858
                     /gene="tnpR"
                     /locus_tag="HS092_RS25315"
                     /old_locus_tag="HS092_25325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn4401 family resolvase TnpR"
                     /protein_id="WP_004152391.1"
                     /translation="MKTGQTTASSSTPVGFPDADSLAALRGWYAGLSSRAAVEHYLAAR
                     KAAGQSARGMLGHIRRQLIVFANSRHRPDLADVFQHAPEERQGRAGAVERAIRLLPTLP
                     APKPQISDSIDQWLSPRTVRALQAYGIHTLSALTVRIPRRRGWWKAIEGLGMTGARQVE
                     AFFAAHPQLTERARALIPIIQPSGIVPWEQLRLPNEVDGSQGNFRAPRNACTLNATNDY
                     EAVQAWLALHETVATQRAYRKEAARLILWAIVERGRALSSLTTDDAIAYRAFVRHPTPR
                     ERWVGPPRPRDSVEWRPFSGGLSARSAAYALTVLSALFRWLIEQRYVLANPFAGVKVRG
                     HALRPALDTARGFTEGEWLLLRAIADGLEWSYGWSEPAAQRLRFMLDFGYATGLRASEL
                     VGAVLGNVRLDEHGDHWLHLVGKGGKPGKVALPPLAYTALDQYLAQRQLPVSRERWNPK
                     TPLVGSLGEDGNAGITGPHLWCVLRRFFTQAAEAIEADHPVAAEKLRRATPHWMRHSHA
                     SHALARGAQLTAVRDNLRHASIATTSMYLHGDELERARQMRQAFGARHVKRGVD"
                     /name="tnpR"
     gene            2256..2483
                     /locus_tag="HS092_RS25320"
                     /old_locus_tag="HS092_25330"
     CDS             2256..2483
                     /locus_tag="HS092_RS25320"
                     /old_locus_tag="HS092_25330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_224019247.1"
                     /translation="MFGFSPGELMNLCPDERLLFVRMISAMLRRSGGDAGAVMFEAYRH
                     IVSDTNQARRSYMLDLLESVRHDYVHGGYT"
                     /name="hypothetical protein"
     gene            complement(2531..2719)
                     /locus_tag="HS092_RS25325"
                     /old_locus_tag="HS092_25335"
     CDS             complement(2531..2719)
                     /locus_tag="HS092_RS25325"
                     /old_locus_tag="HS092_25335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165998.1"
                     /translation="MTKQESAALNMAKFIRAQSLLLLEKLDVLDLDDEAADCERMHEQA
                     EALYQKLSARFGIQDGE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2974..3276)
                     /locus_tag="HS092_RS25330"
                     /old_locus_tag="HS092_25340"
     CDS             complement(2974..3276)
                     /locus_tag="HS092_RS25330"
                     /old_locus_tag="HS092_25340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005161566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="WP_023207139.1"
                     /translation="MSSMVYCRGCGKEIHETAKSCPHCGATNASSGSGEKSRIAAALLA
                     FFLGGFGVHKFYLGKIGQGFLYLIFCWTFIPAIIAFIEFIIYLCDSDEKFARKYG"
                     /name="TM2 domain-containing protein"
     gene            complement(3584..3697)
                     /locus_tag="HS092_RS25335"
                     /old_locus_tag="HS092_25345"
     CDS             complement(3584..3697)
                     /locus_tag="HS092_RS25335"
                     /old_locus_tag="HS092_25345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072202106.1"
                     /translation="MHEPPVQSDCCAFSGNYQLESNPERHDKAPLAAATGN"
                     /name="replication initiation protein"
     gene            4425..5744
                     /locus_tag="HS092_RS25340"
                     /old_locus_tag="HS092_25350"
     CDS             4425..5744
                     /locus_tag="HS092_RS25340"
                     /old_locus_tag="HS092_25350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152397.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISKpn6 family transposase"
                     /protein_id="WP_004152397.1"
                     /translation="MAASYLPYRPDQSYLLPPSLGEWLPEGHLAYFISETVDTLDLSAF
                     HARYAGGGPGNQPFHPAMMVKVLIYGYASGVFSSRKLARKLYEDVALRVLAAGNFPAHR
                     TLSDFRALHLTELENLFVQVVQLARECGLVKLGTIAVDGTKVKANASRHKAMSYKRMKP
                     AEDELHCEIKALLDRAKATDDQERNEPELDIPAEISRREKRLEAIQAAKARLEARQREA
                     DQARGRSEDDGRRPRHPDGSDKGGGSYKREFGVPDDRDQESFTDPDSRIMKHAGGGSEQ
                     SYNGYTAVDAEHQIIVAAELTNCAADSQALLGMLAAVQANTGEMPAQTLADAGFRSEAV
                     LAKVADHHGDVIVALGREGREDAKVNAKTHPHTAAIAAKLKTEQGDAAYRRRKSIVEAP
                     NGWIKAVMGLRQFSMRGLDKVQAEWKLVCMALNLRRMAYL"
                     /name="IS1182-like element ISKpn6 family transposase"
     gene            complement(5994..6875)
                     /locus_tag="HS092_RS25345"
                     /old_locus_tag="HS092_25355"
     CDS             complement(5994..6875)
                     /locus_tag="HS092_RS25345"
                     /old_locus_tag="HS092_25355"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015256839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbapenem-hydrolyzing class A beta-lactamase
                     KPC-2"
                     /protein_id="WP_004199234.1"
                     /translation="MSLYRRLVLLSCLSWPLAGFSATALTNLVAEPFAKLEQDFGGSIG
                     VYAMDTGSGATVSYRAEERFPLCSSFKGFLAAAVLARSQQQAGLLDTPIRYGKNALVPW
                     SPISEKYLTTGMTVAELSAAAVQYSDNAAANLLLKELGGPAGLTAFMRSIGDTTFRLDR
                     WELELNSAIPGDARDTSSPRAVTESLQKLTLGSALAAPQRQQFVDWLKGNTTGNHRIRA
                     AVPADWAVGDKTGTCGVYGTANDYAVVWPTGRAPIVLAVYTRAPNKDDKHSEAVIAAAA
                     RLALEGLGVNGQ"
                     /name="carbapenem-hydrolyzing class A beta-lactamase KPC-2"
     gene            complement(7262..8041)
                     /gene="istB"
                     /locus_tag="HS092_RS25350"
                     /old_locus_tag="HS092_25360"
     CDS             complement(7262..8041)
                     /gene="istB"
                     /locus_tag="HS092_RS25350"
                     /old_locus_tag="HS092_25360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISKpn7 family helper ATPase
                     IstB"
                     /protein_id="WP_004152394.1"
                     /translation="MNLQHERIDALCQQLKLERVATHYPVLAQQAVREDFSFTDFLEHL
                     LRHEAGCRQQRSRELLTRMACFPGIKTLEDYDFSFNPGVPKALVQELGSLAFVERAENV
                     VLIGPSGIGKTHLAIALGYKAAQAGVKTRFITAADLMLQLSTAQRQGRLKEYFNRAILG
                     PRLLVIDEIGYLPFGREEANLFFQVVAKRYEKGAMILTSNLPFSQWADAFAGDTTLTAA
                     MLDRLLHHAHILTLSGESYRLKDKRKAGVVRKNSKPE"
                     /name="istB"
     gene            complement(8038..9063)
                     /gene="istA"
                     /locus_tag="HS092_RS25355"
                     /old_locus_tag="HS092_25365"
     CDS             complement(8038..9063)
                     /gene="istA"
                     /locus_tag="HS092_RS25355"
                     /old_locus_tag="HS092_25365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032490293.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="WP_004199214.1"
                     /translation="MLTQEQTVEIRVLARQGHSIRHIARTLGVSRNTVRRYLRDPSVAR
                     YQPREPRPTKLGPFESYLRQRVEQAHPIWLPATVLNREIRAQGYGGGLSLLRAFLATLK
                     PARREAGPAVRFETEPGRQLQADFVVLRRARSPMSAFVATLGYSRMTFVTFVPDESFES
                     VRDSLLLAFDYLGGVPREVLFDNMKTVVLERDAYGDGKHRFHPGLLQLADDLGFRIRLC
                     RPYRAQTKGKVERFNRYFRESFYNPLLTRMKGTGLLLDCAAANRRVRDWLADEANVRVH
                     ATLNERPIDRWRQEREHLQPLPSRVRRDEAPLLDNSLRPVPLESLQHPLSVYDAIGEAC
                     R"
                     /name="istA"
     ARG             complement(5998..6875)
                     /name="blaKPC-2"
                     /gene="blaKPC-2"
                     /gene_name="carbapenem-hydrolyzing class A beta-lactamase
                     KPC-2"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          1912..2077
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.617"
                     /coverage="96.0"
                     /evalue="1.5900000000000001e-58"
     Typing          complement(3589..4071)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.041"
                     /coverage="80.0"
                     /evalue="0.0"
ORIGIN
        1 taggacgctg agtagcttgt tcttcttgct catggcccgt tcttgctatc ttgaggtttc
       61 gtagaaacat ggggcatttt cgattctact gaaataatcg ttattatcgc gttactcgct
      121 tttcgaatcg caacctgtcc atatgaaaac gggccagact acggcttcga gttctacccc
      181 agtcggcttt cccgatgcgg attcgctggc tgccttgcgc ggctggtacg cgggactcag
      241 ctctcgcgcg gccgttgaac actacctggc ggcacgcaag gccgccggtc aatcagcgcg
      301 agggatgctg gggcatatcc gccgccagct catcgttttc gccaacagcc gccaccggcc
      361 ggatctggcc gacgtgttcc agcacgcccc cgaagagcga caggggcgcg cgggtgccgt
      421 cgaacgcgcg atccggctct tgcccacgct gccggcgccg aagccccaga tcagcgacag
      481 catcgaccag tggctgtctc cgaggaccgt cagggcactg caggcgtacg gtatccacac
      541 cctctcggcg ctgaccgtgc gcattccccg ccggcgtggc tggtggaaag ccatcgaggg
      601 actcggcatg acaggcgccc ggcaggtcga ggctttcttt gccgcacatc cgcagctcac
      661 cgagcgcgcc cgggccctga tcccgattat tcagcccagc ggcatcgtgc cgtgggagca
      721 gttgcgtctg ccgaacgaag tcgacggctc gcagggcaat ttccgggccc cgcgcaacgc
      781 ttgcacgctg aatgcgacca acgactacga ggccgtgcag gcctggctgg cgctgcacga
      841 gactgtcgcc acacagcgcg cctatcgcaa ggaggctgcg cggctaatcc tgtgggccat
      901 cgtcgaacgc ggacgggcgc tatcatccct gaccacggac gatgcaatcg cgtaccgggc
      961 ctttgtccgg cacccgaccc cgcgcgagcg ttgggtgggg ccacctcgac cacgcgattc
     1021 ggtggaatgg cgccccttct cgggcggctt gtccgcacgc tcggcagcgt atgcgctgac
     1081 ggtgctctcg gcgctgttcc ggtggctcat cgagcagcgc tatgtgctgg ccaacccttt
     1141 cgccggcgtc aaggtacgcg gtcacgcctt gcggcccgca ctggatacgg cgcgaggctt
     1201 taccgaggga gaatggctac tgctgcgcgc cattgcagac ggcctggaat ggtcctacgg
     1261 ctggtccgag ccggcagcac aacgcctgcg cttcatgctc gacttcggct atgccacggg
     1321 cctgcgcgct agcgaactgg tcggcgccgt cctgggtaat gtccgcctcg acgagcacgg
     1381 tgaccactgg ctgcatttgg ttggcaaagg tggcaaacca ggtaaggtgg ccttgccgcc
     1441 gctcgcgtat acggcgctgg accaatactt ggcacagcgt caactgccag tcagccggga
     1501 acggtggaat ccgaaaacgc ccctggtggg cagtcttggg gaagacggta atgccggcat
     1561 caccggccct cacctttggt gcgtgttgcg aagattcttt acgcaagccg ctgaggccat
     1621 tgaggccgat catccggtcg ccgccgaaaa gctgcgccgt gccacgcctc attggatgcg
     1681 tcacagtcat gccagccatg cgttggcccg gggagctcag ctcaccgcgg tgcgcgacaa
     1741 cctacgccat gcgtcgatcg ccacgacgtc gatgtatttg catggcgatg agctcgagcg
     1801 cgccaggcag atgcggcagg cttttggcgc tcgtcacgtc aagcgaggag tcgactgaat
     1861 tttcatcata cggaatgacg gaattttctg ggattcccgc ttagaacccc aggaagcgga
     1921 atatctcctg tctcatatat tccgcttacg ctctgctgct gcattttcct cctgccacat
     1981 gaagcacttc actgattccc gcacccgtgc ctacatagta agccagtata cactccgcta
     2041 gcgctacgtg actggttcag ggctgcgccc cgacacccgc taaaagcact gacgcgcctg
     2101 cggcttgccc aaccccgcgc cgtccgggaa agaatttccc gatcgacccg acgtttctgt
     2161 ttacctgtga tgttaacgtt tggatctttt tcccgggttt acctattttc ttctaatgaa
     2221 gcgttcacag ctgacgttaa tagatgggtg ctttcatgtt tggtttttca ccaggagaac
     2281 ttatgaacct ttgccccgat gaacgtctgc tctttgtgcg gatgatttcc gctatgcttc
     2341 gccgttcggg cggtgatgcg ggtgctgtta tgtttgaggc gtatcgccat attgtgtccg
     2401 ataccaatca agctcgtcgt tcctacatgc tcgacctgct cgaaagcgtc aggcatgact
     2461 atgtacacgg tggatataca tagttgccct ggatacgctt ttcgtctaag tgagcgttct
     2521 tttcgtctct ttattcgccg tcctgtatac cgaagcgtgc gctcagcttc tgataaagcg
     2581 cttctgcctg ttcgtgcatt cgctcacagt cggctgcctc gtcatcaaga tcgagcacgt
     2641 cgagtttttc gagcagcagc agcgactgcg cccggataaa ttttgccatg ttcagtgcgg
     2701 ctgattcctg ttttgtcata cggctttttc ccggttttat ggtgatcgca gactgaaatc
     2761 ttacttcatt cacttgagtg catagccatg caggtacgca gaaatcatca ggggcgtttt
     2821 ttctcgatat gggcacagaa aggtgtatgt ttcaggccgg agtcctcaga aacgcgctga
     2881 gggcgtctca ggcggtgcgg gcaacggatg ttatggtaaa tctgcctcac agactggtac
     2941 aaaaaaagcc ccgcagggct tttttgctat gagttagcca tattttcttg cgaatttttc
     3001 atcggaatcg catagataaa taataaactc aataaacgcg atgatcgcgg gtatgaatgt
     3061 ccagcaaaaa attagataca ggaatccctg tcctatttta cctaagtaaa atttatgtac
     3121 gccaaatcca ccaaggaaga atgctaatag cgcggctgcg attctacttt tttcacctga
     3181 acctgaagag gcgtttgtgg ctccgcagtg tgggcatgac tttgctgttt cgtgtatttc
     3241 cttgccgcat cctcggcagt aaaccattga actcattttt tatcctcgag cttattttta
     3301 gttgggatgc ataatatccg atgttttata acgatatgca tcctatatgc tcgggtggga
     3361 tagatttcca ttatggtgaa ggatcttctt gagatccttt ctgtagcgtc gtaatctctt
     3421 gctctgtaaa cgaaaaaacc gcccagcaag gcggtttttc gaaggttctc agagcagcaa
     3481 ctctttgaac cgtggtaact ggcttacgag gagcgcagga accaaatctg ttctttcagt
     3541 ttagccttaa caggcgcata acttcaagac taactcctct aaatcagtta ccagtggctg
     3601 ctgccagtgg cgctttgtcg tgtctttccg ggttggactc aagttgatag ttaccggaaa
     3661 aggcgcagca gtcggactga acggggggtt cgtgcataca gtccagcttg gagcgaactg
     3721 ccttcccgga actgagtgtc aggcgtggaa tgagataaac gcggccataa cagcggaatg
     3781 acaccggtaa accgaaaggc aggaacagga gagcgcacga gggagctacc agggggaaac
     3841 gcctgggatc tttatagtcc tgtcgggttt cgccgccact gatttgagcg tcagatttcg
     3901 tgatgcttgt caggggggcg gagcctatgg aaaaacggct tcgccgcggc cttatcgttt
     3961 ccctgttaag tttcttcctg gcatctccca ggagatttcc gccccattcg taagcccgca
     4021 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gggggggtaa
     4081 gcgggaaccc cagaaaattc cgccattccg cattgtggaa ttttttgggg gggtggtccg
     4141 cggcatgacg acccgccaaa ggctaccgca tctatctgag gcgctgtggc gcgacgctga
     4201 aaccccgggg aattccgcta ctccagttcc aggtccgcaa tagttcgtcc aggtcgggtg
     4261 gcgtacgccg gaaacgccga tttttccgca accgtttctg cgttgtcggc aaggtggtct
     4321 cagagagcgc tcaagaagcg gtttttctcg tatgtttagc tacggtggcc taggagcctg
     4381 cgcggcagaa gccaaatcgg cggaagatcg aggacgatac ggggatggct gccagttacc
     4441 ttccttaccg acccgaccaa tcctatctgc tgcccccttc tctgggagag tggctacctg
     4501 aagggcatct tgcctacttc atcagcgaga ctgtcgatac gctggacttg agcgcattcc
     4561 acgcccggta tgccggcggt ggtccgggca atcagccgtt tcatccggcg atgatggtca
     4621 aagtgctgat ctatggttac gcgagcggcg tcttctcttc gcgcaaacta gccaggaagc
     4681 tgtacgagga tgtcgcgttg cgtgtgctgg ccgctggaaa cttcccggcc caccgcacgc
     4741 tgagtgactt ccgtgcccta cacctgaccg agcttgagaa tttgttcgtt caggtggtgc
     4801 aactggcgcg cgaatgtggg ctggtgaagc ttggcacgat cgcggtcgac ggcaccaagg
     4861 taaaagccaa cgccagccgc cacaaggcga tgagctataa gcgcatgaag ccggccgagg
     4921 acgaattgca ttgcgagatc aaggcgctgc ttgatcgcgc caaggctacc gacgaccagg
     4981 agcgtaacga gccggagctg gacattcctg ccgagatttc tcgccgcgag aagcgcctgg
     5041 aggcgatcca ggcggcaaag gcgcgcctgg aagcgcgcca gcgtgaagcg gaccaggccc
     5101 gggggcgcag cgaagacgat ggccgccggc ctcgccatcc ggatggctcg gacaagggcg
     5161 gtggctcgta caaacgcgag tttggtgtgc cggatgaccg tgatcaggaa agcttcaccg
     5221 atccggacag ccggatcatg aaacacgccg gtggtggctc cgagcagagc tacaacgggt
     5281 acacagcggt cgatgccgag caccagatca tcgtggcggc ggagttgacc aactgcgccg
     5341 cggacagtca ggcgctgctg ggcatgctgg cagcagttca ggccaacacc ggagaaatgc
     5401 cggcccagac gctggcggat gcgggattcc gtagtgaggc tgttctggca aaggtcgccg
     5461 atcaccacgg cgatgtcatc gttgccctcg gccgcgaggg acgtgaagat gccaaggtca
     5521 atgccaagac ccatccgcat acggcggcga ttgcggcgaa attgaaaacg gagcaaggcg
     5581 atgcagctta ccgccggcgc aagtcgatcg tggaggctcc gaatggttgg atcaaggcgg
     5641 tgatgggatt gcgtcagttc agcatgaggg gcctggacaa ggtgcaagcc gagtggaagc
     5701 tcgtctgcat ggcgctaaat ctgaggcgaa tggcgtatct gtgagggcga aggttaaatg
     5761 gggcggctca aatgcacccc agtcgtcata acacgccgcg cgccgcaaga ttggtatcct
     5821 tggcgccgac gccttgccaa ttgcagagag cgccgccgcc atcgtcagtg ctctacagaa
     5881 aaccggtcac acggctctgc cgcgcagact cctagcctaa atgtgacagt ggttggtaat
     5941 ccatgccgcc cgcaagggcg gcggtggtgg gccaatagat gattttcaga gccttactgc
     6001 ccgttgacgc ccaatccctc gagcgcgagt ctagccgcag cggcgatgac ggcctcgctg
     6061 tgcttgtcat ccttgttagg cgcccgggtg tagacggcca acacaatagg tgcgcgccca
     6121 gtgggccaga cgacggcata gtcatttgcc gtgccataca ctccgcaggt tccggttttg
     6181 tctccgactg cccagtctgc cggcaccgcc gcgcggatgc ggtggttgcc ggtcgtgttt
     6241 ccctttagcc aatcaacaaa ctgctgccgc tgcggcgcag ccagtgcaga gcccagtgtc
     6301 agtttttgta agctttccgt cacggcgcgc ggcgatgagg tatcgcgcgc atcgcctggg
     6361 atggcggagt tcagctccag ctcccagcgg tccagacgga acgtggtatc gccgatagag
     6421 cgcatgaagg ccgtcagccc ggccgggccg cccaactcct tcagcaacaa attggcggcg
     6481 gcgttatcac tgtattgcac ggcggccgcg gacagctccg ccaccgtcat gcctgttgtc
     6541 agatattttt ccgagatggg tgaccacgga accagcgcat ttttgccgta acggatgggt
     6601 gtgtccagca agccggcctg ctgctggctg cgagccagca cagcggcagc aagaaagccc
     6661 ttgaatgagc tgcacagtgg gaagcgctcc tcagcgcggt aacttacagt tgcgcctgag
     6721 ccggtatcca tcgcgtacac accgatggag ccgccaaagt cctgttcgag tttagcgaat
     6781 ggttccgcga cgaggttggt cagcgcggtg gcagaaaagc cagccagcgg ccatgagaga
     6841 caagacagca gaactagacg gcgatacagt gacatcaacg atattccttg tttgaaggtg
     6901 gagctaggtg tagcggccgg atgtaatcag gccgctacag ctgggattat caccgagcgg
     6961 caagccgctc cctctccaga ggctgtaacg gctgtaactg ccccggatgg gcacacatcg
     7021 ggttggtaag tccctggcac cacaacgtcc agcgctattg aaaaacgaat caggtcggcg
     7081 ttcatgggtc ggccacctcg cctgacctcg gcatccacgt tacggacggc ctcaggaagt
     7141 cctggccaag ccccgactat tggggcgcga agatagcacc gagggtacgc cggtgtcaac
     7201 accaactgaa aactgaccca ctttacccct gaatcaccaa agtaatactg acccacctgg
     7261 atcattcggg tttggaattt ttcctgacca ctcccgcctt cctcttgtcc ttcaagcggt
     7321 agctttcgcc gctcagggtc aggatatggg catggtgcag cagccgatcc agcatcgcgg
     7381 ctgtcagggt ggtatcgccg gcaaacgcgt cggcccactg gctgaacggc aggttcgagg
     7441 tcaggatcat cgcccctttc tcatagcgct tcgccaccac ctgaaagaac aggttcgctt
     7501 cctcgcgtcc gaaaggcagg tagccgatct catcgatcac cagcagccgt ggaccgagga
     7561 tggcgcggtt gaagtattcc ttcagccgcc cttgtcgctg cgccgtgctc agttgcagca
     7621 tcaggtcggc tgccgtgatg aagcgtgtct tgaccccggc ttgcgcggcc ttgtaaccca
     7681 aggcgatggc gaggtgggtc ttgccgatgc cagagggtcc gatcagcacc acgttctcgg
     7741 cccgttcgac aaacgccaga ctgcccagct cctgcaccag tgccttgggc acgccggggt
     7801 tgaagctgaa gtcatagtcc tccagggtct tgatgcccgg gaagcaagcc atccgcgtca
     7861 gcagttcgcg gctgcgctgc tggcgacacc ccgcttcatg ccgcaacagg tgttccagga
     7921 agtcggtgaa gctgaagtcc tcgcgcaccg cctgctgcgc caggacgggg taatgcgttg
     7981 ccacccgttc gagtttgagt tgctggcaca gggcgtcgat ccgctcatgc tggaggttca
     8041 tcggcaggcc tccccaattg cgtcatagac agacagtggg tgctggagag attccagtgg
     8101 taccgggcgg agactgttat ccagcagggg ggcctcgtcg cgccgcacac gggatggcaa
     8161 gggttgcagg tgttcccgct cctgccgcca acggtcgatg gggcgctcgt tcagggtggc
     8221 gtggacccgc acattcgcct cgtctgccaa ccagtcacgg acgcgtcggt tggcagcagc
     8281 acaatcgagc agcagccccg tgcccttcat ccgggtcagc aacgggttgt agaagctctc
     8341 gcggaagtag cgattgaagc gctcgacctt gcctttggtc tgggcccgat acgggcgaca
     8401 cagccggatc cggaacccca ggtcatcggc cagttgaagc aagccggggt gaaaacggtg
     8461 cttgccgtca ccgtaggcat cgcgttccag caccacggtc ttcatgttgt cgaacaacac
     8521 ctcgcggggc acaccgccca gatagtcaaa agccagcaac agggagtccc gcacggactc
     8581 gaaggattcg tccggtacga aggtgacaaa cgtcatgcgg ctgtatccca gcgtggcgac
     8641 gaaggccgac atgggggatc tggcccggcg caagaccacg aaatcagcct gcaactgccg
     8701 tccgggctcg gtctcgaaac ggacggccgg acctgcttcg cggcgggcgg gcttgagcgt
     8761 ggcgaggaag gcgcgcaata acgagagacc gccgccatac ccttgagcgc ggatttcccg
     8821 attcaggacg gtggcaggca gccagatggg gtgggcctgc tcgacccgtt gccgcagata
     8881 agattcaaat ggccccagtt tggtggggcg tggttcacgg ggctggtatc gcgctaccga
     8941 cggatcgcgg aggtagcggc gcacggtatt gcgcgagacc cccaacgtgc gggcgatgtg
     9001 ccggatggag tggccctgtc gggcaagaac tctgatttcc acggtttgct cctgggtcaa
     9061 catcgacggc ctcgaaaagg ccgccaaact agcccaggtg gatcagtttt acttcgctgg
     9121 ggtgggtcag ttttacactg ctgctaacac ggaaccgctg aaaattccgt cactccagtt
     9181 ccaggcctgc gacgatcgca tcgagatcga tcgtcttgcc gctgctttga aaggtgtagt
     9241 gcccgttgag caggatgtgc cgccatgccg caggcgaaat ttttgtgatg agtgccagcg
     9301 ccttgctgtt gccggccgcc tcgtacttgg tcaccagccg cgacaagatt gccgagttgt
     9361 agaaaatgat cgtgttggcg atcagccgtg cgcactggtt gctgatctcg atttcaatgt
     9421 cggtgcgacc ggtcagttcc ttcttaccgc cgacctgggc aatggccgag cgtagctgat
     9481 ggtaggactc gaggcgattt tgcgagcggt gcacgttgcg ttccagttgc ggatcgcgca
     9541 ggtagcgaag cgtgtagatg ctgcggatga gcttgtcgaa ctcgaagatc gcgcgccgcg
     9601 tcgggttcgt cgtggtgtag gtgcacagct tgcggatcag cgtgccctgc gtcatttcct
     9661 tcagcccgag tgtggcgacg atccggtcga tgttcggctt ctcgcgatgg atgagatcgc
     9721 agtcgatttt cccgactggc cgaatcaggc acgcctgata ctgtgctgga tcgtcagcgc
     9781 agtagagttc tttcaattgc gcgttgaggt cggtgaagcg cggctcgaac cgccggccaa
     9841 accaatgcag gatggcgaag ttggccttgt tgacgctgtg catgtcgccg gtgatcgcgg
     9901 tcggcatgat gtccgacgtg ttgcgatacc agatgtcgaa cacgtggtgg gcctcgtagt
     9961 cgtgcgcgcc gatcaggtag ccgttgagcg ggatgtggtt gcacagcagc gtgtaggcga
    10021 ccacgccctt gccgcgcccg aagtacttgc gcgaatagcg cgccttcacg gtcggccgtt
    10081 cgacgctgaa tttctgcccg tcgacggcac cgtacagcgt ttcgaggtcg aacgagtagt
    10141 atgggaagat cggtagcgtg gcgatggcgt tgctgatgca gtcgttggcc gcgtgcagcg
    10201 ttgcctggcg caggtactgc tggtagccgc tatccagcac gtggtacggg atgtcgctgg
    10261 tgcgcgccat gacgtggttg ccgtggttca tcgcttgcgc gatgatgacc gccatcaggc
    10321 tgtcagcgtc ggcgaccttc ttcgcgtagc gcggctgcat gggcgtgaac gccgacagaa
    10381 acttgcactc gtcgttgacg aagcgaaaca cgtcggccac gtcgcagaac ggaagcttct
    10441 cgtagaatgc cttttcgcgc gctttctcat tctcgctctt gggcttgcgc caggtcagtt
    10501 tctgcgtgtc cttgttgtat tcgaggtgcg tcagcttgcc ctgtttcagc tcgcggttga
    10561 acgccagcca ctgcgtgcgc agttcggcag tcagcgcatc gagttgggca tggacgggtt
    10621 gctgcaggaa ggggatctcc atttttgcga gcacatcgat cttcctgtcc atctcaacca
    10681 gttcatctga gaagtgccgg tgttgcaggc tgtcgtcgag gtagagttca cccgattgga
    10741 gacgcttcct gatctggcgg tacagccaga actcgtagcg gtcagcgtgc aggccggttg
    10801 gtttaccgtc ggcgtcgaag gtcagcaggt acggccgcaa gcgtttcgga agtgtcgccg
    10861 cgggccactc gtcgagtggc cgttgcgaca gccgctgctg cttggcaaaa acacctttgg
    10921 cccaggccag cgcggcaagc catggactgt ctggattgga gccggagagg tcgagcgcaa
    10981 cgtacagtgg acgaagatgg cggcgcatgc ggtcggccag cccgtccacc gcctgccact
    11041 gcaaggccag cttgctcgcc ggcttcacgc tcatacgctg cgccgtggtt tgcagcgtat
    11101 cgcgaggcat gatcttgtag gcgcgctgcc gcacctcgcc gaagggcgtg ggatcggcca
    11161 cgctatcatc aacgtagagt gacagcaggc ggcctacccg cggcgtttcc tgctgacggc
    11221 gcacctgctc gtcggcaaag gattgtttgg caccagcgct gctttcgtcc tccaactgct
    11281 tcatgtggta cgccatcgca tcgaccaggt tgtcggagag ctgccggtag cgtacccagg
    11341 catagcacag caggtagagt tgggtctgat cggccttcag attgcgcagg tcgtggacgg
    11401 tgtagaagtt tgccaggctc gcgtagtaca gcaggttctg ctgcgagacg tcgagtttag
    11461 gcagcagtgt cttggcgatt gcgtgcagcg gctccagcgt ggcgcgcttt tcgcgctcgc
    11521 ggaccatctg gcgccagccg aaattcttgg cgtcctgctt gagcgccgcc agttgcgaca
    11581 gggtgtcatc acggaccaga agctggctca gcgcggcctt ggcagattcg tccaacgctt
    11641 gctccagcag attgcccagg cgccggcgct cggcggatag cgtttcgctg accaactctt
    11701 gcaaggtcgt atagccgggc cggataatct tgtgctcatt gagccagacg atcagctcgg
    11761 cggcgacgaa ccccggcgtt acgtcacgcc gcacggtccg tgcggcctgc tgctcgagct
    11821 gcggcaggaa ggcggccgac cacggccagt agccgaatag tttggcgatc tgctcgcgct
    11881 gggtgtagcg ttcatgcttg gtgatcgcct tgggttcgaa cacctcgccg tggaaatagc
    11941 ggctcagcac gaaagcgcaa tcgtcctcga cttcgtccca gtcgaaactg aagaaggcat
    12001 gcttggcctt gaagtagccg atctgcaaga ggcaatagac cttggcatga agactgggcc
    12061 ggctgttggc aagcgccaac tcggtttctg tcacagccag atattccagc tgctgcgcat
    12121 catcgaactc cggcagg
//
