LOCUS       NZ_CP054275             2118 bp    DNA     circular CON 08-DEC-2023
DEFINITION  Raoultella ornithinolytica strain OHMEATKPC2_NDM5 plasmid pOSUMEAT5,
            complete sequence.
ACCESSION   NZ_CP054275
VERSION     NZ_CP054275.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15079954
            Assembly: GCF_021496525.1
KEYWORDS    RefSeq.
SOURCE      Raoultella ornithinolytica
  ORGANISM  Raoultella ornithinolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Raoultella.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Wittum,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUN-2020) College of Veterinary Medicine, The Ohio
            State University, 1920 Coffey Rd., Columbus, OH 43210, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: NOV-2019
            Assembly Method                   :: Unicycler v. v0.4.8
            Assembly Name                     :: OSU_Rorn_KPC2NDM5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Illumina MiSeq; PacBio RS
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/08/2023 12:16:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,519
            CDSs (total)                      :: 5,396
            Genes (coding)                    :: 5,259
            CDSs (with protein)               :: 5,259
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 64 of 137
            Pseudo Genes (incomplete)         :: 89 of 137
            Pseudo Genes (internal stop)      :: 21 of 137
            Pseudo Genes (multiple problems)  :: 31 of 137
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP054275.1.
            Bacteria and source DNA available from Thomas Wittum, The Ohio
            State University College of Veterinary Medicine, 1920 Coffey Rd.,
            Columbus, OH 43210.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Raoultella ornithinolytica"
                     /mol_type="genomic DNA"
                     /strain="OHMEATKPC2_NDM5"
                     /isolation_source="Retail Pork"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:54291"
                     /plasmid="pOSUMEAT5"
                     /geo_loc_name="USA: Ohio"
                     /collection_date="2018"
     gene            complement(170..286)
                     /locus_tag="HRV96_RS27465"
                     /old_locus_tag="HRV96_27360"
     CDS             complement(170..286)
                     /locus_tag="HRV96_RS27465"
                     /old_locus_tag="HRV96_27360"
                     /inference="COORDINATES: protein motif:HMM:NF033853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein"
                     /protein_id="WP_142375627.1"
                     /translation="MDWISLAIAIALLVVAVYSGISYFREKKTISRVFRSKR"
                     /name="small membrane protein"
     gene            1318..1509
                     /locus_tag="HRV96_RS27470"
                     /old_locus_tag="HRV96_27365"
     CDS             1318..1509
                     /locus_tag="HRV96_RS27470"
                     /old_locus_tag="HRV96_27365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041614.1"
                     /translation="MNKQQKTAVNMAKFIQDQSLLLLDRLNELDLDHEADFCEKLHEDA
                     ERLYRSLSARLNEQQDGA"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(824..1443)
                     /name="rep_cluster_2327"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.929"
                     /coverage="99.0"
                     /evalue="2.55e-171"
ORIGIN
        1 ttccgcgcta tgcacttgcg cgcatactca tgcatgccgt aaaaacagag cctgcgcgtt
       61 tctggcgggt tttcgggtgg tttgttgcct gttttaccgg tttcccgtca gaaacgccct
      121 gaggccgttt tcgcggtgca cgtaagaagg gattatggta aatagggcgc tatcgcttac
      181 tcctaaatac acgactgatt gttttttttt ctctgaagta tgagattcct gagtagacag
      241 ctacaacaag tagagcaatg gcgatagcaa gtgaaatcca gtccataaat gcctgattaa
      301 tttaataaaa acagcctgtt atgatttcat aaagataata tcatcttttt tttagcatga
      361 tactgtcgtt acttattgtg gtgagatgaa acatagccac tgtttttgta tgtgtttgtt
      421 aaggccgtta agattattct taatcactga ttttgtacat atcatcaaaa taaaaataaa
      481 aaaaactaat gcccccgtgg tgggtatata acaaatcacg gggtgcaggt cagaactacc
      541 ccaaaacttt gaatgatcgg gtggcagact aaatgaaatc ttgagattca ttcagcctcg
      601 tcgtaatctc tttctctgta aacgaaaaaa ccgccctgca aggcggtttt tcgaaggttc
      661 agtaagttgg ggaacttctg aaccgtggta acagtcttat gcgagacgtg tcacccaagc
      721 tgtccttcta gtgtagccgt agtaagggca ccacttcaag aactctcaca atatctctcg
      781 cacatcctgt taccagtggc tgctgccagt ggcgcatttg catgtctttc cgggttggac
      841 tcaagacgat agttaccgga taaggcgcag cggtcggact gaacgggggg ttcgtgcata
      901 cagtccagct tggagcgaac tgcctacccg gaactgagtg tcaggcgtgg aatgagacaa
      961 acgcggccat aacagcggaa tgacaccggt aaaccgaaag gcaggaacag gagagcgcac
     1021 gagggagcca ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacca
     1081 ctgatttgag cgtcagattt cgtgatgctt gtcagggggg cggagcctat ggaaaaacgc
     1141 cccgcagggg cctggccttt tcgccctcgt ccttagcttt cgttatgctt accgttacgt
     1201 tatctgctta tcctccctcc gctcgccgca gactcatgac cgaacggagt gagcgaatga
     1261 gcgaggaagc ggaaaaccat cagaacgtac agtaagcact ttcgcgccgg ggccttcatg
     1321 aacaaacaac aaaagactgc cgttaacatg gctaaattca tccaggatca gtccctgctt
     1381 cttctggaca ggcttaatga gctggatctg gatcatgagg ctgatttctg cgagaaactc
     1441 catgaggatg cggaacgcct ttaccgaagc ctgtcagcgc gcctgaatga gcagcaggac
     1501 ggtgcgtaat gtctccgttc tgggtattac cggagtaata ccggcatcgc cttaattttc
     1561 attgtcttag cgcttaacat tattaccctg gtaataccca caatccctgt attttttctt
     1621 ttttatttat ttttcatgaa gttgcgatga aaatcgcggc tgcgttaggt gtatgaccat
     1681 tttgaggttg aaaaataatc aaccatggcg ggtgatttac gcaggttaag gggagaacgt
     1741 gacaaaaaaa gcgttcctca ctgtatcgtt catgcctgca tgaacgggtt attcttcctg
     1801 actacagaat tacattgctc cttcagttca tacatcactt ttacatcact tttgcatcat
     1861 tccttcagcc tatacatcac ttttacaaca cttttgcatc acttttacat tattccttca
     1921 gctcatacgt cacttttacg tcagttccat cctctgtgcc atttgtacat ccgaccccct
     1981 tcctgaacgt gctgaaaacg gtttttccgg ctgtgccggc aacgcctcac ggccacgcaa
     2041 taaattgcgt tcccgttcac cgttttcggg tgggaagaat gccggaaaca ggacttaccc
     2101 cggctgagtt gcgtgaat
//
