LOCUS       NZ_CP054274             3271 bp    DNA     circular CON 08-DEC-2023
DEFINITION  Raoultella ornithinolytica strain OHMEATKPC2_NDM5 plasmid pOSUMEAT4,
            complete sequence.
ACCESSION   NZ_CP054274
VERSION     NZ_CP054274.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15079954
            Assembly: GCF_021496525.1
KEYWORDS    RefSeq.
SOURCE      Raoultella ornithinolytica
  ORGANISM  Raoultella ornithinolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Raoultella.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Wittum,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUN-2020) College of Veterinary Medicine, The Ohio
            State University, 1920 Coffey Rd., Columbus, OH 43210, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: NOV-2019
            Assembly Method                   :: Unicycler v. v0.4.8
            Assembly Name                     :: OSU_Rorn_KPC2NDM5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Illumina MiSeq; PacBio RS
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/08/2023 12:16:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,519
            CDSs (total)                      :: 5,396
            Genes (coding)                    :: 5,259
            CDSs (with protein)               :: 5,259
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 64 of 137
            Pseudo Genes (incomplete)         :: 89 of 137
            Pseudo Genes (internal stop)      :: 21 of 137
            Pseudo Genes (multiple problems)  :: 31 of 137
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP054274.1.
            Bacteria and source DNA available from Thomas Wittum, The Ohio
            State University College of Veterinary Medicine, 1920 Coffey Rd.,
            Columbus, OH 43210.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Raoultella ornithinolytica"
                     /mol_type="genomic DNA"
                     /strain="OHMEATKPC2_NDM5"
                     /isolation_source="Retail Pork"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:54291"
                     /plasmid="pOSUMEAT4"
                     /geo_loc_name="USA: Ohio"
                     /collection_date="2018"
     gene            complement(429..908)
                     /locus_tag="HRV96_RS27445"
                     /old_locus_tag="HRV96_27340"
     CDS             complement(429..908)
                     /locus_tag="HRV96_RS27445"
                     /old_locus_tag="HRV96_27340"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.3"
                     /GO_function="GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]; GO:0008080 - N-acetyltransferase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_065365727.1"
                     /translation="MANFSCGVTHLDEFLKTDLHKMQGRSLLKGYVLLSDDETPEIMGY
                     YTLSGASFERGHLSNRLRKKIPYSNMPAVLLGRLAIDQRIQRKGFGELLIVDAIHKVRA
                     TAGQIGIYAMFVEAHPGASSFYTRMGFIPSSMADDNKTFFYPANQFDDLIFGDKC"
                     /name="GNAT family N-acetyltransferase"
     gene            complement(950..1234)
                     /locus_tag="HRV96_RS27450"
                     /old_locus_tag="HRV96_27345"
     CDS             complement(950..1234)
                     /locus_tag="HRV96_RS27450"
                     /old_locus_tag="HRV96_27345"
                     /inference="COORDINATES: protein motif:HMM:NF020265.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="WP_044316655.1"
                     /translation="MNAVRKERFEFRLPEAAKRRIEEAAVISKVSVSQFVMESATFRAE
                     TVINEHRRLIVEEDVWEQVMAALERPPAPTQSMCKAFERYNSEESWICD"
                     /name="DUF1778 domain-containing protein"
     gene            complement(2387..2788)
                     /locus_tag="HRV96_RS27455"
                     /old_locus_tag="HRV96_27350"
     CDS             complement(2387..2788)
                     /locus_tag="HRV96_RS27455"
                     /old_locus_tag="HRV96_27350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_032744268.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFQQPKGEHHSGQVAEQRDTAFADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2788..2979)
                     /locus_tag="HRV96_RS27460"
                     /old_locus_tag="HRV96_27355"
     CDS             complement(2788..2979)
                     /locus_tag="HRV96_RS27460"
                     /old_locus_tag="HRV96_27355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032744269.1"
                     /translation="MTKQQKTALNMAKFLQHQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          2..237
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.958"
                     /coverage="87.0"
                     /evalue="3.02e-100"
     Typing          3028..3244
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.52"
                     /coverage="87.0"
                     /evalue="3.22e-55"
ORIGIN
        1 tcgtgcgctc tcctgttcct gccattcggt ttacccgtgt cattccgctg ttatggccgc
       61 gtttttctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac
      121 tgtatgcacg aaccccccgt tcagtccgac tactgcgcct tatccggtaa ctatcgtctt
      181 gagtccaacc cgaataggca cgacaaagcg ccactggcag cagccactgg taactgatta
      241 gaggagttag tcttgaagtt atgcgccggt taaggctaaa ctgaaaggac tgatttggtg
      301 actagctcct ctttagccag ttaccttggt ttagtgaact tgccggttca ccaaccttaa
      361 aaaagccacc ttgccgggtg gttttttttt actttcacat atatctcata gatatatgtg
      421 aaagtactct agcatttatc gccaaagata agatcatcaa actgattagc tgggtagaaa
      481 aatgttttat tatcatcagc cattgatgat ggaatgaatc ccattcttgt gtaaaaagat
      541 gaagctccag gatgcgcctc aacaaacatg gcatagatac caatctgtcc agcagtagcc
      601 ctcactttat ggatagcatc gacaattagt aactcaccaa aaccttttcg ctgaattcgt
      661 tgatcgatag caagacgacc taacagcact gccggcatgt tcgagtaagg aattttcttc
      721 ctcaggcggt tactcaaatg ccctcgttcg aacgatgcac cgctcagcgt gtagtacccc
      781 ataatttctg gagtttcatc atcagaaagc agaacgtagc cctttaatag gctacgcccc
      841 tgcattttgt ggagatcggt tttcaaaaac tcatctaaat gtgtgactcc acagctaaag
      901 ttcgccatgc tgtactcagc acacaagtca aaaggttgaa aaccagggat taatcgcaaa
      961 tccaactctc ctcagagtta taacgctcaa acgctttaca catactttgt gttggtgctg
     1021 gagggcgttc aagggcagcc atgacttgct cccaaacatc ctcttcaacg atcaagcggc
     1081 gatgttcatt aataactgtt tcagctctaa aagtcgcaga ctccattaca aattgagata
     1141 cggacacttt agaaataaca gcagcttctt caatacggcg cttcgcagct tcgggcagac
     1201 gaaactcgaa ccgctccttt cttactgcgt tcataacaac tcctgagcta atgctcacag
     1261 tggtttatac cacatgacga tatgcacggt acggtaattt accgtacagg taatttgagg
     1321 tgattttgat ctgagaaact atgccagtca agctaatgaa gggtgagggt agtatttttt
     1381 gagaaaaaaa cttaaatttc ggaaaaataa ccttaaaaaa caacaagtta catatggcaa
     1441 tgacacttag ggggcattgg tagttcttaa gaactaccaa tgccccctaa gtgtcattgc
     1501 cagtgtattt tatcattttt ttgcatgtaa atctaccgct attttgaacg aatgtatggg
     1561 ccaaatcaca cagcattcag gggatgtgca ttacggtatt ttgcgcccca taaatccacc
     1621 cacgcactcc aaaaccagcc actgatccag atttaccata acgtctcatt acgcgcaccg
     1681 ctcaaacgcc ccagaaacga ttctgggccg taatccatca aatcctacat cttaccccct
     1741 gaaaacgcct caaaatcgcc ccagggcttt ttacgggcat gaattgttat gcaccagact
     1801 gactggtggc ggatttcagc ctgaattcac gccagacaac tcaaaacccg caccaaaccc
     1861 gactgaaaac ggtgaacggg gacgcaataa attgcgtggc cgtgaagcgt tgccggcgca
     1921 gccggaaaac cggttttcag cacgttcagg aatcgggttc gtcgacggcc ggcaatcacg
     1981 ttatggcaag tcgccggacg actcaccaca cgattcaccg gcgattcgac tcgtgacttg
     2041 actctagagt cgccatggtc aaaaagccac caacccggca agtcgactct agagtcaagt
     2101 catcataacg tactgattat atggcgtgat tcacatatga atcgtgggca agtcatcggc
     2161 gattcgccat agagtcgtcc acgactcgga ttcaccttac aaaaaataaa attgcgtaat
     2221 ttaaaataga taactgattg aaaataaagg tctatctgca aaattttaat tttgcgtagt
     2281 gtgtgggtat ttttagtggt gaggagcgtt cagggatctg acgatttttg ggaaggttca
     2341 ggaactcccc ggaatggccg gagagcaaaa tggaagcatg gcgggatcag gattttttcc
     2401 agaatttcca ccatggcgcc ggggattcag ttaattttgc ctggtgattt tcaagaagga
     2461 gcatggcctg tttcaggctg tcaatgtgcg aatccttgga caaaagtaag gtgttcaggt
     2521 gttcaatctc ctgtttcaga tgacgaatca cctgttcact ctggtcagca aaggctgtat
     2581 cacgctgttc agcgacctgt ccagaatggt gttcgccctt cggttgctgg aagggaccaa
     2641 agacacgtaa aagctctgaa gtatctacca tttttgcatt gcgttcaccg ctacaggtgg
     2701 acaggtcacc tgctttaatt aacctgtgca gcgttgttct actcttaccc gtcagtcggg
     2761 cagcctcact aatagacact ctggccatca gttaccatcc tgtaatgcat ccagtctcga
     2821 ggacagtgta cggaaaagat gctccgcatc atcgtggagt ttctcacaaa ggtcggcctc
     2881 tgcatcaaga tcaagctcgt taagcttctc cagcaggagc agagactgat gctggaggaa
     2941 tttagccatg ttcagagctg ttttctgttg ttttgtcata agaagcctca gagcgtcagt
     3001 gctcaatgta gggatgttct cttttccgct tcctcgctca ctcagtcgct acgctcctgc
     3061 cgttcggctg cggcgagcgg tcgcaactca cgtataagag cataagacga cagggctgat
     3121 aaccggaggg gacaagataa ggtcgcggcg ttgccgtttt tccataggct ccgcccccct
     3181 gacaagcatc atagaaattg acgctcaaat cagtggtggc gaaacccgac aggactataa
     3241 agatacctat catttctgat aggtggctcc c
//
