LOCUS       NZ_CP054168            10552 bp    DNA     circular CON 10-DEC-2023
DEFINITION  Escherichia coli strain STO_Bone1B plasmid pBone1B_3, complete
            sequence.
ACCESSION   NZ_CP054168
VERSION     NZ_CP054168.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15006794
            Assembly: GCF_021498165.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10552)
  AUTHORS   Li,F., Luthje,P., Shafeeq,S., Ahl,M., Akerlund,B. and Romling,U.
  TITLE     Characterization of Escherichia coli isolates from a chronic bone
            infection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10552)
  AUTHORS   Li,F., Luthje,P., Shafeeq,S. and Romling,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAY-2020) Department of Microbiology, Tumor and Cell
            Biology, Karolinska Institutet, Solna vagen 9, Stockholm 17165,
            Sweden
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 1210x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/10/2023 02:32:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,743
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 101 of 271
            Pseudo Genes (incomplete)         :: 154 of 271
            Pseudo Genes (internal stop)      :: 74 of 271
            Pseudo Genes (multiple problems)  :: 53 of 271
            Pseudo Genes (short protein)      :: 4 of 271
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP054168.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="STO_Bone1B"
                     /isolation_source="bone infection of foot"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pBone1B_3"
                     /geo_loc_name="Sweden: Stockholm"
                     /lat_lon="59.351331 N 18.026000 E"
                     /collection_date="2005-01-01"
                     /collected_by="Karolinska University Hospital"
     gene            join(10099..10552,1..104)
                     /locus_tag="HRS04_RS00970"
                     /old_locus_tag="HRS04_00955"
     CDS             join(10099..10552,1..104)
                     /locus_tag="HRS04_RS00970"
                     /old_locus_tag="HRS04_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001235713.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTDR
                     EGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGDMG
                     QMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATE
                     IAHQLSIARSTVYKILEDERAS"
                     /name="recombinase family protein"
     gene            287..1147
                     /locus_tag="HRS04_RS00975"
                     /old_locus_tag="HRS04_00960"
     CDS             287..1147
                     /locus_tag="HRS04_RS00975"
                     /old_locus_tag="HRS04_00960"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="WP_000027057.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
                     /name="broad-spectrum class A beta-lactamase TEM-1"
     gene            complement(2073..2306)
                     /locus_tag="HRS04_RS00980"
                     /old_locus_tag="HRS04_00965"
     CDS             complement(2073..2306)
                     /locus_tag="HRS04_RS00980"
                     /old_locus_tag="HRS04_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137445.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2313..2831)
                     /locus_tag="HRS04_RS00985"
                     /old_locus_tag="HRS04_00970"
     CDS             complement(2313..2831)
                     /locus_tag="HRS04_RS00985"
                     /old_locus_tag="HRS04_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067500.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<2869..3519)
                     /locus_tag="HRS04_RS00990"
                     /old_locus_tag="HRS04_00975"
                     /pseudo=""
     CDS             complement(<2869..3519)
                     /locus_tag="HRS04_RS00990"
                     /old_locus_tag="HRS04_00975"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3509..3832)
                     /locus_tag="HRS04_RS00995"
                     /old_locus_tag="HRS04_00980"
     CDS             complement(3509..3832)
                     /locus_tag="HRS04_RS00995"
                     /old_locus_tag="HRS04_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_052895110.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPTL
                     APPLLRQLAAIGNNLNQTARKVNCGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGTR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3897..4088
                     /locus_tag="HRS04_RS01000"
                     /old_locus_tag="HRS04_00985"
     CDS             3897..4088
                     /locus_tag="HRS04_RS01000"
                     /old_locus_tag="HRS04_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_042634456.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENH"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(5200..5556)
                     /locus_tag="HRS04_RS01005"
                     /old_locus_tag="HRS04_00990"
     CDS             complement(5200..5556)
                     /locus_tag="HRS04_RS01005"
                     /old_locus_tag="HRS04_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075993.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_052895113.1"
                     /translation="MSQATRKPSTVGDIILYEYLEPSGLKINDLAEMLNVHRNSASALV
                     NNNRKLSTDMAFRLAKAFDTTAKFWLNIQQSVDLWEVTSSPRLQEEIDRVIPAHEIIER
                     KHIVDNSEKKHAIA"
                     /name="HigA family addiction module antitoxin"
     gene            complement(5760..6077)
                     /locus_tag="HRS04_RS01010"
                     /old_locus_tag="HRS04_00995"
     CDS             complement(5760..6077)
                     /locus_tag="HRS04_RS01010"
                     /old_locus_tag="HRS04_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001183575.1"
                     /translation="MQQDHEHDDCRIQFRDDWLYQFYKYGKANRKIPTAISASLGRKLD
                     MLNAADKVGDLRSPPGNRFENLNPPLTGYSSIRVNEKYRLIFKWGENGIEDLYLDPHDY
                     R"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            6264..6506
                     /locus_tag="HRS04_RS01015"
                     /old_locus_tag="HRS04_01000"
     CDS             6264..6506
                     /locus_tag="HRS04_RS01015"
                     /old_locus_tag="HRS04_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_052895112.1"
                     /translation="MALVRSGNKSKIFEADKKFNSVFDTEYQPGETCEYEGIYKCTGCG
                     KEVTIAGGKTIPPQNHHQHTPEQGNIRWQLFVYAQ"
                     /name="hypothetical protein"
     gene            complement(6930..9935)
                     /locus_tag="HRS04_RS01020"
                     /old_locus_tag="HRS04_01005"
     CDS             complement(6930..9935)
                     /locus_tag="HRS04_RS01020"
                     /old_locus_tag="HRS04_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
                     /protein_id="WP_001143760.1"
                     /translation="MPVDFLTTEQTESYGRFTGEPDELQLARYFHLDEADKEFIGKSRG
                     DHNRLGIALQIGCVRFLGTFLTDMNHIPSGVRHFTARQLGIRDITVLAEYGQRENTRRE
                     HAALIRQHYQYREFAWPWTFRLTRLLYTRSWISNERPGLLFDLATGWLMQHRIILPGAT
                     TLTRLISEVREKATLRLWNKLALIPSAEQRSQLEMLLGPTDCSRLSLLESLKKGPVTIS
                     GPAFNEAIERWKTLNDFGLHAENLSTLPAVRLKNLARYAGMTSVFNIARMSPQKRMAVL
                     VAFVLAWETLALDDALDVLDAMLAVIIRDARKIGQKKRLRSLKDLDKSALALASACSYL
                     LKEETPDESIRAEVFSYIPRQKLAEIITLVREIARPSDDNFHEEMVEQYGRVRRFLPHL
                     LNTVKFSSAPAGVTTLNACDYLSREFSSRRQFFDDAPTEIISRSWKRLVINKEKHITRR
                     GYTLCFLSKLQDSLRRRDVYVTGSNRWGDPRARLLQGADWQANRIKVYRSLGHPTDPQE
                     AIKSLGHQLDSRYRQVAARLCENEAVELDVSGPKPRLTISPLASLDEPDSLKRLSKMIS
                     DLLPPVDLTELLLEINAHTGFADEFFHASEASARVDDLPVSISAVLMAEACNIGLEPLI
                     RSNVPALTRHRLNWTKANYLRAETITSANARLVDFQATLPLAQIWGGGEVASADGMRFV
                     TPVRTINAGPNRKYFGNNRGITWYNFVSDQYSGFHGIVIPGTLRDSIFVLEGLLEQETG
                     LNPTEIMTDTAGASELVFGLFWLLGYQFSPRLADAGASVFWRMDHDADYGVLNDIARGQ
                     SDPRKIVLQWDEMIRTAGSLKLGKVQVSVLVRSLLKSERPSGLTQAIIEVGRINKTLYL
                     LNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYTDGQEDQLGTLGLVTNA
                     VVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINMLGHYSFTLAELVTKGHLR
                     PLKEASEAENVA"
                     /name="Tn3-like element Tn3 family transposase"
     ARG             288..1144
                     /name="blaTEM-1"
                     /gene="blaTEM-1"
                     /gene_name="broad-spectrum class A beta-lactamase TEM-1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(4174..4261)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.876"
                     /coverage="100.0"
                     /evalue="3.19e-37"
     Typing          4272..4830
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.078"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1970..3519)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.183"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgacgcttca tcagaagggc actggtgcaa cggaaattgc tcatcagctc agtattgccc
       61 gctccacggt ttataaaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata
      121 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
      181 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
      241 acaataaccc tggtaaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
      301 ttttcgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
      361 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
      421 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
      481 aatgatgagc acttttaaag ttctgctatg tggtgcggta ttatcccgtg ttgacgccgg
      541 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
      601 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
      661 aaccatgagt gataacactg ctgccaactt acttctgaca acgatcggag gaccgaagga
      721 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
      781 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc
      841 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
      901 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
      961 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     1021 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     1081 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     1141 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     1201 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     1261 acgtgagttt tcgttccact gagcgtcaga ccccgattta taagcttttt ggtcgcgcag
     1321 aaccccagaa agatgaggac aaactcggtt tctagaacca taaggcagca tcaaaacggc
     1381 attcatttac cataacatcc gttaactgcg gccttgtcat aaaagaaatg gatattggag
     1441 acaatgtgag caataagatc tatagaacag tgagccatgt agatggttgg aatggccttc
     1501 ctcagtacga aatcagggga gacaaaatct acaggacagt gagccatgta ggtggttgga
     1561 atggtcttcc tcaatacgaa atcaggggaa ataaaatcta caggacagtg agccatgtag
     1621 atggttggaa tggtcttcct caatacgaaa tcaggggaaa taaaatctac aggacagtga
     1681 gtcatgtaaa cggttggaac ggtcttccgc aatatgagat cagataattt gcggggcaaa
     1741 ccctgcaaaa agaaatcata tttaccataa catctgtttt gtacgcaccg ccagaatacg
     1801 ttcatcgcga tcacggcagc agacaggtaa aaatggcaac aaaccacccg aaaaactgcc
     1861 gcgatcgcgc ctgataaatt ttaaccgtat gaatacctat gcaaccagat ggtacaggcc
     1921 acattacccc cacttaatcc actgaagctg ccatttttca tggtttcacc atcccagcga
     1981 agggccatcc agcgtgcgtt cctgtatttc cggctgacgc tcccgttcca gggataacac
     2041 atgttcgcgc tcctgtatca accgttcctc tcttatctcc agttctcgct gtataactgg
     2101 ctcaagcgtt ctgtctgctc gctcaagtgc tgcacctgct gactcaactg catgacccgc
     2161 tcgttcagca tcgcgttgtc ccgttgcgta agcgaaaaca tcttctgcaa ttccacgaag
     2221 gcgctctccc attcgctcag ccgctgcata tagtcctgtt gcagctgctc taaggcgttc
     2281 agcaaatgtg tttccagctc tgtcactctg tgtcactcct tcagatgtac ccactctttc
     2341 ccctgaaagg gaatcacctc cgctgatttc ccgtacggaa ggacaaggaa tttcctgttc
     2401 ccgtcctgca caaactccac gccccatgtc ttcgcgttca gtttctgcaa tgtctcttcc
     2461 tgcttcctga tttcttccag gttcgcctgt atcctcccgc caagatacca gagcgtcccg
     2521 ccactcgcgg taaacaggag aaagactatc cccagtaaca tcatgcccgt attccctgcc
     2581 agctttaaca cgtccctcct gtgctgcatc atcgcctctt tcaccccttc ccggtgtttt
     2641 tccagcgatt cctctgtcga ggcggtgaac agggctatag cgtctctgat tttcgtctcg
     2701 tttgatgtca cagcctcgct tacagattcg ccgagcctcc tgaactcgtt gttcagcatt
     2761 ttctctgtag attcggctct ctctttcagc tttttctcga actccgcgcc cgtctgtaaa
     2821 agattgctca taaaatgctc ctttcagcct gatattcctc ccgccgttcg gatctgcaat
     2881 gctgatactg cttcgcgtca ccctgacaac ctcaactccc acatctgtga gtgccagaat
     2941 cacatcctga cggtctttta tctctccggc acgataaagc acatcaagcc cccgtgtaac
     3001 gctctcagca agcgcctgtt tcgtttccgg cagattatca gggagtgtca gcgtcctgcg
     3061 gttctccggg gcgttcgggt catgcagccc gtaatggtga ttaaccagcg tctgccaggc
     3121 atcaattctc ggcctgtctg cgcggtcgta gtacggctgg aggcgttttc cggtctgtag
     3181 ctccatgttc ggaatgacaa aattcagctc aagccgcccc ttgtcctggt gctccaccca
     3241 cagaatgctg tactgatttt tttcgagacc gggcatcagt acacgctcaa agctcgccat
     3301 cactttttca cgtcctcccg gcggcagctc cttctccgcg aacgacagaa caccggacgt
     3361 gtatttcttc gcaaatggcg tggcatcgat gagttcccgg acttcttccg gattaccctg
     3421 aagcaccgtt gccccttccc ggttacgctc ccttcccagc aggtaatcaa ccggaccact
     3481 gccaccacct tttccccggg catgaaactt aactatcatc ccgcgtcccc tgttccctga
     3541 cggccagacg cagccggcgt agctcgtccc cgatggccat cagtgcggcc accacctgaa
     3601 cccggtcacc ggaagaccac tgcccgcagt tcaccttcct cgctgtctga ttcaggttat
     3661 tcccgatggc ggccagctga cgcagtaacg gcggtgccag tgtcggcagc ctcccggaac
     3721 gggcaaccgg ctcccccagg cagacccgcc gcatccatac cgccagttgt ttaccctcac
     3781 aacgttccag caaacgagca tgttcatcat cagtaacccg tatcgtgagc atcctctcgc
     3841 gtttcatcgg tatcattacc cccatgaaca gaaatccccc ttacacggag gcatcagtga
     3901 ctaaacagga aaaaaccgcc cttaacatgg cccgctttat cagaagccag acattaacgc
     3961 tgctggagaa gctcaacgaa ctggacgcag atgaacaggc agatatctgt gaatcgcttc
     4021 acgaccacgc cgatgagctt taccgcagct gcctcgcacg tttcggggat gacggtgaaa
     4081 accactgaca catgcagctc ccggagacgg tcacagcttg cctgtgagcg gatgccggga
     4141 gcagacaagc ccgtcagggc gcgtcagcag gttttagcgg gtgtcggggc gcagccctga
     4201 cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagtgcggat
     4261 tgtatgaaaa gtgcgccatg ccgggtgtga aatgccgcac agatgcgtaa ggagaaaatg
     4321 cacgtccaag cgcttttccg cttcctcgct cactgactcg ctacgctcgg tcgttcggct
     4381 gcggcgagcg gtactaactc acacaaaaac ggtaacacag ttatccacag aatcagggga
     4441 taaggccgga aagaacatgt gagcaaaaga ccaggaacag gaagaaggcc acgccgcagg
     4501 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaaccgacgc tcaagtcaga
     4561 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     4621 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     4681 gaagcgtggc gctttctcat agctcacgct gttggtatct cagttcggtg taggtcgttc
     4741 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     4801 gtaactatcg tcttgagtcc aacccggtaa gacacgcctt aacgccactg gcagcagcca
     4861 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     4921 ggcctaacta cggctacact ggaaggacag tatttggtat ctgcgctctg ctgaagccag
     4981 ttaccaggtt aagcagttcc ccaactgact taaccttcga aaaaccgcct ccccaggcgg
     5041 ttttttcgtt tacagagcaa gagattacgc gtacagagca cggatctcaa gaagatcgta
     5101 agaagaaagt taacatgagt gtttttatcc ctcaaaaaga aggcttctat aaatagaagc
     5161 cttctttttg agggataact tatatttttt taaaaaaaat catgcaatag catgtttctt
     5221 ctcagaatta tcaacaatgt gtttcctttc tatgatttca tgagcgggaa tcactctgtc
     5281 tatttcttct tgcaatcgag ggctactagt aacctcccat aaatcaacag actgctgtat
     5341 atttagccag aactttgcag tggtatcgaa ggctttagcc aacctaaaag ccatatcggt
     5401 agacagcttt ctattattgt taactaaagc actggcagag tttctatgca cattcaacat
     5461 ttcagccaag tcattgattt ttaaaccact tggttcgaga tattcataaa gaataatatc
     5521 ccctacagtt gatggctttc tagttgcttg ggacatgctt tactcctaaa ttttttactt
     5581 ctcatctgtt agatgaggaa gataagtatt tcgtctatta ccagctgtag cgatgagtaa
     5641 ttatcttaaa tatagtcacg tcatttagtt tattaatcat tatttgtaat gacattttag
     5701 tgtaggtgag ggaacgcctc acctacttgt tttataagat aacagccacg acctgttatc
     5761 tatcggtaat catgtggatc gagatacaaa tcctcgattc cgttttcacc ccacttaaat
     5821 attaagcggt acttctcatt cacccgtatg gatgaatacc ctgtcaatgg agggttgaga
     5881 ttctcaaatc ggtttcccgg gggagaccgc aaatctccaa ctttatcagc agcattcagc
     5941 atatccagct tgcgacctaa tgaagcacta atcgcagttg gaatctttcg attggcttta
     6001 ccatatttgt aaaactggta tagccaatcg tctcgaaact gaatgcggca atcatcatgt
     6061 tcatgatctt gctgcatttt tcttttacct caatttttgc cgcaacttgg caatttttta
     6121 gatcaccttc ttacctccga tatagtacag caaacacaac aaatgcacaa cagcacagtg
     6181 cacttgtgca accaataacc atactgtaga atgcacccac acacagtgca gatgtgcaaa
     6241 atagagggta atttggaggt tacatggctc ttgttcgttc aggaaacaaa agcaagattt
     6301 ttgaagcaga taagaagttt aactcggttt ttgacactga atatcagccg ggagaaacat
     6361 gtgaatatga agggatttat aaatgtaccg gttgcggtaa agaagtaact atcgcaggtg
     6421 ggaaaacaat cccaccacaa aaccaccacc aacatacccc cgagcaaggg aacattcgtt
     6481 ggcaattgtt tgtttatgct cagtgaggaa tagaaaatgg cagatcgtcg accgctcaac
     6541 ccacacgttg tcgttcctaa accaggcggc ggaactcaac ctcgtgaccg taacaatgac
     6601 ggaagggtca gaggaaaacg ctcagatgca ggaaagccaa gaaaataaca gcaagccgcc
     6661 agcctggcgg cttttttatt tatatctaca gctgacactg atttaccata gcatccgctg
     6721 tatctactca gtcttgatag gagtttttat gagtaatgaa ttagacagcc tttcagaagt
     6781 agttaaagga ctcacaaaga tagagctgcc tggcggcggt ggcggtgaaa ccgatagccc
     6841 tgtagttgag gcagctatag ctgttggcat tgtaggggct gctgcatatg ggatttgggg
     6901 tctgacgctc agtggaacga aaactcacgt taagcaacgt tttctgcctc tgacgcctct
     6961 tttaatggtc tcagatgtcc tttggtcacc agttctgcca gcgtgaagga ataatggccg
     7021 agcatattga tatgtccgtg gcaaagcggg gagaggcgtg cgatatcttc atcattcagt
     7081 gtttcaccct gcgcccggag atgatccagg gctgcctgca tataaatagt gttccataac
     7141 acgacggcgt tagtgaccag ccccagtgtg cccagttgat cttcctgacc gtcggtatat
     7201 cgttttctta tctcaccttt ttgaccgtga cagatggctc tggcaacggc atggcgactt
     7261 tctccccgat taagctgggt cagaatgcgc cggcggtaat cttcatcatc aatataatta
     7321 agcagataca gcgttttgtt gatgcgcccc acttcaatga ttgcctgagt cagtccggaa
     7381 ggacgttcac ttttcagcaa tgaacggacc agcactgaaa cctgtacttt gcccagcttc
     7441 agggagccag cggtccggat catttcgtcc cactgaagga ctatttttcg gggatctgat
     7501 tgccctctgg caatatcatt cagcacgcca tagtcggcat catggtccat tcgccagaaa
     7561 accgaagcac cggcatcagc caggcgtgga gaaaactggt atcccagcag ccagaaaagg
     7621 ccaaagacaa gttcgctggc acctgctgta tcggtcataa tttcggttgg attcagcccg
     7681 gtctcctgtt ccagaagacc ttccagcaca aagatagagt ccctcagcgt ccccggtata
     7741 acgatgccat gaaagccgga atactgatcg gacacaaagt tgtaccaggt gatccctctg
     7801 ttattaccaa agtatttgcg gttcggtccg gcattgattg ttctgactgg cgtaacaaag
     7861 cgcattccat ctgcagatgc cacttctcct ccaccccata tctgtgccag tggcagcgtt
     7921 gcctgaaaat caaccagtct ggcattagcg ctggtgatag tttcagcccg cagatagttc
     7981 gcttttgtcc agttcagccg gtgtcgggtc agtgcaggaa catttgatct gatcagtggt
     8041 tccagaccga tattgcaggc ttcagccatc agcacggcgc tgatgctgac gggcagatca
     8101 tcaactctgg cactggcttc actagcatgg aaaaactcat cagcaaatcc ggtatgggcg
     8161 ttaatttcga gcagcaactc cgttaaatcc accggaggga gtagatcact gatcattttg
     8221 ctcagtcgtt tcagactgtc cggctcatca agactggcga ggggagaaat tgtcaaccgg
     8281 ggcttcgggc cagaaacatc gagttcgaca gcctcatttt cgcaaagacg tgcagcaacc
     8341 tgtctgtaac gactatcaag ctgatgaccc agagatttta ttgcttcctg cgggtctgtc
     8401 gggtgcccca aagaacgata aaccttaatc cggtttgcct gccagtcagc accctgtagt
     8461 aatcttgcac gaggatctcc ccaccggtta ctgccggtaa cgtagacatc cctccgcctc
     8521 agactatcct gcagtttact gagaaagcag agcgtgtatc ccctgcgggt gatatgtttt
     8581 tccttgttaa tcaccagccg tttccatgac cgactgataa tttccgttgg tgcgtcgtca
     8641 aaaaactgcc gccgtgagct gaactcccgg ctgaggtagt cacaggcatt cagagtggta
     8701 accccggcag gtgcggatga aaatttaacg gtattcagca gatggggcag gaaacgacga
     8761 acgcgcccgt actgctccac catttcttca tgaaaattat cgtctgaggg ccgggcaatt
     8821 tcacggacaa gcgtgatgat ttcagccagc ttttgccttg ggatgtagct gaacacctca
     8881 gcacgaatcg attcgtccgg tgtttcttct ttcagcaggt acgaacatgc gctggcgagc
     8941 gccaatgcag atttatccag atccttcagc gagcggagcc gttttttctg cccaatcttt
     9001 ctggcgtcac ggatgataac ggccagcatg gcgtccagaa cgtccaatgc atcatccagc
     9061 gccagcgttt cccatgcaag gacaaaggca accagaaccg ccatcctttt ctgcggtgac
     9121 atcctggcaa tattgaacac cgaagtcata ccagcataac gtgcgagatt tttcaggcgc
     9181 acagccggga gtgtactcag gttttcagca tgcaggccaa aatcgttcag agttttccag
     9241 cgttcaattg cttcattaaa cgccggacca ctgatggtca cagggccctt tttcagtgat
     9301 tccagtaaag acaggcggct gcaatcagtt ggccccagca gcatctccag ctgtgaacgc
     9361 tgttcggctg acggtatcag tgccagtttg ttccacaggc gcaacgtcgc cttttccctt
     9421 acctctgaaa tcaaccgggt cagcgtagtg gctccgggga gaataatacg atgttgcata
     9481 agccaccctg tcgccagatc gaaaagcagg ccaggacgtt cgttgcttat ccagctccgg
     9541 gtatataaaa gacgggtaag gcgaaatgtc cagggccagg caaattcacg atactgatag
     9601 tgctgacgta tcagcgctgc atgctcacgg cgggtatttt ccctctgacc gtattctgca
     9661 agaacggtga tatcacgaat cccgagctgt ctggcggtaa aatgccggac gccggaagga
     9721 atatgattca tatcggtgag gaaggtgccc agaaaacgga cacatccaat ttgcagggca
     9781 atgcccagac ggttgtgatc acctctgctt tttccgataa attccttgtc tgcttcatca
     9841 aggtgaaaat atcgtgccag ctgaagctca tccggttcac cggtgaatct gccatagctt
     9901 tcagtctgct cagtggtcag aaagtcaacg ggcatatcgg cctccctgcc tgacgggcat
     9961 ttagtaacat ttttccaacc gtacgaaatg ttataaatta tcggacatcg taaaactgtt
    10021 acattaatat gtctattaaa tcgtaaattt gtaataatag acatgagttg tccgatattc
    10081 gatttaaggt acatttttat gcgacttttt ggttacgctc gggtctcaac cagtcagcag
    10141 tctcttgatc ttcaggtcag agcactcaaa gacgcaggtg tgaaagcaaa ccgtatattt
    10201 accgataagg catccggcag ttcaacagac cgggaagggc tggatttgct gaggatgaag
    10261 gtggaggaag gtgatgtcat tctggttaag aagctcgacc gtcttggccg cgacactgcc
    10321 gatatgatcc aactgataaa ggaatttgac gctcagggcg tggcagtccg gttcattgat
    10381 gacgggatca gtaccgacgg tgatatgggg caaatggtgg tcaccatcct gtcggctgtg
    10441 gcacaggctg aacgccggag gatcctagaa cgcacgaatg agggccgaca ggaagcaaag
    10501 ctgaaaggaa tcaaatttgg ccgcaggcgt accgtggaca ggaacgtcgt gc
//
