LOCUS       NZ_CP053877            10060 bp    DNA     circular CON 10-DEC-2023
DEFINITION  Klebsiella pneumoniae strain KPN142 plasmid pn142_2, complete
            sequence.
ACCESSION   NZ_CP053877
VERSION     NZ_CP053877.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14987945
            Assembly: GCF_021498005.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Lv,F., Wang,Y., Luo,Y., Wang,Q.W. and Zhao,G.Z.
  TITLE     Genome sequencing of Pan-drug resistance Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Lv,F., Wang,Y., Luo,Y., Wang,Q.W. and Zhao,G.Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2020) College of Basic Medicine, Guangdong Medical
            University, Wenming East Road, Xiashan District, zhanjiang,
            gunagdong 524023, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: FEB-2020
            Assembly Method                   :: Unicycler v. 0.4.7.
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 203.0x
            Sequencing Technology :: PacBio RSII; Illumina HiSeq 4000
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/10/2023 02:32:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,532
            Genes (coding)                    :: 5,396
            CDSs (with protein)               :: 5,396
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 54 of 136
            Pseudo Genes (incomplete)         :: 81 of 136
            Pseudo Genes (internal stop)      :: 18 of 136
            Pseudo Genes (multiple problems)  :: 15 of 136
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP053877.1.
            Bacteria and source DNA available from China,Dongguan City,
            Guangdong Province.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KPN142"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pn142_2"
                     /geo_loc_name="China: Dongguan City, Guangdong Province"
                     /collection_date="2019-10"
     gene            640..2604
                     /locus_tag="HPY03_RS28035"
                     /old_locus_tag="HPY03_27960"
     CDS             640..2604
                     /locus_tag="HPY03_RS28035"
                     /old_locus_tag="HPY03_27960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            2604..3335
                     /gene="mobC"
                     /locus_tag="HPY03_RS28040"
                     /old_locus_tag="HPY03_27965"
     CDS             2604..3335
                     /gene="mobC"
                     /locus_tag="HPY03_RS28040"
                     /old_locus_tag="HPY03_27965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            3342..3872
                     /locus_tag="HPY03_RS28045"
                     /old_locus_tag="HPY03_27970"
     CDS             3342..3872
                     /locus_tag="HPY03_RS28045"
                     /old_locus_tag="HPY03_27970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(3899..4078)
                     /locus_tag="HPY03_RS28050"
                     /old_locus_tag="HPY03_27975"
     CDS             complement(3899..4078)
                     /locus_tag="HPY03_RS28050"
                     /old_locus_tag="HPY03_27975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(4104..4532)
                     /locus_tag="HPY03_RS28055"
                     /old_locus_tag="HPY03_27980"
     CDS             complement(4104..4532)
                     /locus_tag="HPY03_RS28055"
                     /old_locus_tag="HPY03_27980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(4696..5010)
                     /locus_tag="HPY03_RS28060"
                     /old_locus_tag="HPY03_27985"
     CDS             complement(4696..5010)
                     /locus_tag="HPY03_RS28060"
                     /old_locus_tag="HPY03_27985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(4998..5276)
                     /locus_tag="HPY03_RS28065"
                     /old_locus_tag="HPY03_27990"
     CDS             complement(4998..5276)
                     /locus_tag="HPY03_RS28065"
                     /old_locus_tag="HPY03_27990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(5451..5816)
                     /locus_tag="HPY03_RS28070"
     CDS             complement(5451..5816)
                     /locus_tag="HPY03_RS28070"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(5813..6184)
                     /gene="tolA"
                     /locus_tag="HPY03_RS28075"
                     /old_locus_tag="HPY03_28000"
     CDS             complement(5813..6184)
                     /gene="tolA"
                     /locus_tag="HPY03_RS28075"
                     /old_locus_tag="HPY03_28000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(6458..6703)
                     /locus_tag="HPY03_RS28080"
                     /old_locus_tag="HPY03_28005"
     CDS             complement(6458..6703)
                     /locus_tag="HPY03_RS28080"
                     /old_locus_tag="HPY03_28005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            7030..8715
                     /locus_tag="HPY03_RS28085"
                     /old_locus_tag="HPY03_28010"
     CDS             7030..8715
                     /locus_tag="HPY03_RS28085"
                     /old_locus_tag="HPY03_28010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            8725..8982
                     /locus_tag="HPY03_RS28090"
                     /old_locus_tag="HPY03_28015"
     CDS             8725..8982
                     /locus_tag="HPY03_RS28090"
                     /old_locus_tag="HPY03_28015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            9067..9216
                     /locus_tag="HPY03_RS28095"
                     /old_locus_tag="HPY03_28020"
     CDS             9067..9216
                     /locus_tag="HPY03_RS28095"
                     /old_locus_tag="HPY03_28020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     BGC             2030..10060
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(9320..9898)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2605..3332
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 gtcccctgat taagtctttc tattccctga cgatcaatta tcaggatagg tgaaccatgt
       61 cggtttttcg ctgaacattt tacgttaacc tatctctgtt gcttttttct ccttgcggag
      121 aaatgcaagt catccccgct ggggcgtggc tgacttcagg ggaaaagacc ttgcttacgc
      181 tggctggacg ccaccgacgc acggtctttt cccctctttc tggctctgtc cggctggacg
      241 ccggccacgc cagaaattca cccggccccc gtcaggctca cgcctgacca cccacaaaaa
      301 tccacggtgc tggacgcacc tgacgaagca gcaaagctgc tcctgatttt gcgggtggtc
      361 agccgctcgc tatggcgaac tctgacgggg gccggaacac cggaaaagac cgcactgggg
      421 tgccgccttt tcctctcaca cgctggggcg tgctcgttgt tcctccgggg ctgcgccttt
      481 tccgcatggg gcggaaagag gctggcctcc gcggtgaaag ctgaacgcaa tcaccacgga
      541 cgtgaccgcc gccgccatgc cttggggcat gtccgcggct tgtgcagcgg gggctgcgca
      601 tcacgccggt aaacccgtcg tctgaaggag agagattgca tgtttaatac ggattcgctt
      661 gcctggcagt ggcatatccc ggagctggcc cggcagattt acccgtgggt cagctggctg
      721 taccgggagt attacagtct ggccggtttt tttctggtta tcggcctgtt tttcggtctg
      781 gctgaactct gggccggccg aatgcgtttt gtggggctga gccataaggc gcggcgggag
      841 tatgccgatc gctataagcg cgataataac cggactgtgc cattttcggt gctggcctcg
      901 ctggattcac ccgggcggat ggtcagggca cggcgacatg tctggaacta cgcgtactgg
      961 ctgaccggcg ctgccggtct ggcccttctg ggggggctgg ttgtcgccgt gtttatcgag
     1021 agctggggag aaaccgtcct gcgcccggac ctctatgcga acgcagatta ttacctgctg
     1081 cagccggcta acctgttcgg gatagcctgg tgtgccggag ggcttgtcgc cggcgtcctt
     1141 ctggcgcggc tgctggcgtt cctgctgctt gatggtcatt ttgcggccgc tgacgaggcg
     1201 gtgaacgcga aactgaatca ggagagccgg cgcagcagcc agcggaccgg agaaatgaca
     1261 gacgtccgcc acctgcattt cggggagccg gtaccggtta acgcgctggc cgatttttcc
     1321 acggagcagg cccggaaaca gcaggcggtg tttctgggta aggatgagca gggccagccg
     1381 gtgctggtcc ctcgtgatac ctggcgcaag accaatattc agatcctcgg cctgcccggg
     1441 agcggcaaga gcgcgatggc caccaacgcg ctcattcgct gcgtacgcga tttcggggac
     1501 gctgtggtgt acttcgaccc caaaggggac gcgtgggcgc cgcacgtctt ccgggcgcac
     1561 tgcccggact tcaccctgct ggatctgcgg ccggggaaac cggcgcagct gaatctgttc
     1621 cgcgatctcg accagtacgc cctgaagaat ctgctggttg ccgggtttaa cctgagtgag
     1681 accggcgacg tggcggacca ctaccgcatc agcgagcaga aagcggcgaa gctgattgcg
     1741 gagcagttcc cgcagggggc aaacattcag caggtgctgg cggcggcgta cgcgttgccg
     1801 gaggccctga aaaaggacgt gaaggggctg atcaccaagc tggagaacgt ggccgacctg
     1861 agcgtcctgc agacggacag cggtatcgat gtggcgggga taatcaacgg cggcggctgc
     1921 ctgtacgtca tcggctcgat ggacgatgag gcggtgatcc gggtgcagaa gatgctgttt
     1981 gcccgctgcg cgcaaatcat cattgcgcgg gatgagttcc ggaggtggcc gcacgcgagc
     2041 atcatgctcg atgagattaa atatctgctg tcgaagtacg tgctgaacgc gctggggacg
     2101 ctgcgcagcc gggactgcaa cctgctgctc gcgcaccagt cgctgggcga cttcggtcag
     2161 tgcgggcagg atctgcccgc tgattttgtg aaaaccacgg tactggataa cacgccgatc
     2221 cgctggttct atcgcgcggc cagccaggag tccgcgcagt gggcggcggg ccagaccggg
     2281 gagatccggg tggacgtgga gcgccgccgg gccagccggg aggcggggaa cgtggagcac
     2341 atcagcggcg acagcttcat ccagaaggag gcgcggccgc tgtttgacgt gaacaccctg
     2401 caacacctgc ctgacgggtt cgcggtgatg accgggctgg gcgtggcgcg gctggcgttc
     2461 agcagcccgc tgcgggttga gcgccgggaa atttcgctga aaaccttccc ggtgctggcg
     2521 aaaacggacc cgctggcgga gtacctgcct ccggccgacg gcggcggacc aaaagcggat
     2581 gatgattttg cggggcttta ctgatgacac tggagcagta caacgtcagc cacgcgaagc
     2641 ggcaggcgcg gaacgcggag aaaacacggc tgaccctgcg ctggctgcgg gaagagctgt
     2701 gttccacggc ggagctggtt gcgcgacggc tggggattgc ggcggtacag ccggtttacc
     2761 gcttcctgga cagtctggtg gcgaaagggc tgctggtccg ggcgaaatac ccggttgacg
     2821 gccgtcaggt cagcgtgtgg gggctgacgc cgcacggggt ggcgttctcg ttcgacgagg
     2881 acgagccgct gacggacgtt attccgtttc agccgtcaag ggtgtcggcg gcgcagctgc
     2941 cgcaccggct ggccgtgcag tccctgcgcc tctccatgga gggccggggg gccaccggct
     3001 ggcggtacct gcaccgtatg gccctgaaag ggatgaaagt gccggacgcg ctggcggagc
     3061 tggacggcag aacggtggca tttgaggtag agcgcaccgt caagagccgc aggcggtatc
     3121 aggaagtggt gtccgggtat ctgttcaacc gccgggcgaa cggcatcgat gaaatctggt
     3181 acatctgccc tgaccggccg acgcttgtcc gggtacagcg ggcgattctg gcggtggatg
     3241 aaatcgtgaa tccacagacc ggcgaggccc gcaaaacggt ggagctggac cgggagaggc
     3301 tgttcgcgtg ctttaaattt atgacaacgg agtgagggat tatggcgctg tacatggtat
     3361 ttattgagga cgtggaagac aaagacggcg agcgctgcat tatgccaacc agcgagcatc
     3421 tggaagacat ggtggtgctc gcctggcaga ccggggcgta tgttttctgt tttgtcgacc
     3481 gggcccagca tctggcgatg atgaaatcca tcgggatgta tgacgagcgg ctcaacagcg
     3541 acgaaaaagc cacgtacttt ttcaccggtg aaaatccgca atgggaacgg gacaaaccca
     3601 caggaatgaa gctgtgtgat tacctctatc gaaaggtgga gcggttcgcc atcgagaacg
     3661 gtgtgcaggt ggttgatgcc gggaagtgct caccggagca gattaacgcg atgattgagg
     3721 tggtcagcga gcggcacggg gaaagcgcgg tgaacgagaa gctgaaagcg gagctgatgc
     3781 gggaatataa cagccgctat tttaacgtgc cggtgcctga actgccggag gacaaacccg
     3841 cccgccggtg gtggccgttc ggcaaaaaat aacgccagca ccggcatggc cggtgctgtt
     3901 attcttcccc gagcttatcc cgcatcacct gccagagtgt ttccgcatcc tcgtgaagcc
     3961 gttcgcacat gtcggccggg gtatccatgt ccagctcgtt cagcttttcc agcaacagca
     4021 gcgactgatc cttaatgaat ttagccatat tgagggcagt tttttcctgc ttcgtcatgt
     4081 tctccattcc cttcggtaag ttctcagcta aaccagccgg accagaagcg ttttttcttc
     4141 tgtgtcagtt ctgttgtcaa tctgtccact tcggcctgga gtttccggcg ctcatcatcg
     4201 tgctggcggc gttcttcttc ccgggatgtt ttatcttcca gcatcagggt gacagcctgc
     4261 cggagatcgg ccagttcctt ttgcattgcc agaagctgtt cctgtgggaa atttttttgt
     4321 gtattttcac ggtgtgaaat gccgggaatt tgtttctcag ggatattcac atttccgtat
     4381 acacggatca gttctgacac atccacgaca gggttgtttt tcccgtcacg tgacacggta
     4441 acctttccct gttttacatg attgtatagc gttcttctgg taatgcccgc ggcttgtgct
     4501 gcctgtgaaa ggttgagcaa tgttttcgcc actgtatacc cctgatttta tcgggtgtga
     4561 aaggtgtgta atttctcacg gtaaatgtat cgctgaattt tacccgtttt tccgttcagg
     4621 atgtggtttt ctgacgcgct gcgcttgtca aaccggcgac ggccacgcaa tgaattgcgc
     4681 ccccgtcatc ggttattaga ggctgtctgc ccccggatac ccggattctt tctgatgcct
     4741 gacggtgacg atcacaatct ggtccatttc ccggtcgtgg cggtagagca tcacatagcc
     4801 actgtcgcca aacccgatca ccagttcctt atattccagc ggcagaaacg gcaccgggcg
     4861 gccaatgtct ggcaatgttt tcagttgctg gatagcccgg acgatcacct cacctccttt
     4921 tctggctgcc agccgatttt tggttttgag aaagtcctga aggcgttgta aatcgcgctg
     4981 tgccagcgcg gaaatcgtta cctgtggcat ggcggagcat cctgctcgtt ctcagttccc
     5041 caggtactta tccaggcttc cgcttcttcc gcagtaaggt gcagacccgt ttcctgatag
     5101 tgctgccatg cggcctgtcc gtcccgcagg tactggctgc gcttctcttc ccggtcgatg
     5161 tattcggtga ttgcctccag cattagcgcg tgcgctgagc ggtgccggtc atcggccagc
     5221 gttttcagcc ggtccttaag ttcttcatcg agcctgatgg atgttgctga tgacataaaa
     5281 gcccctttgt agtcatatgt attacacatg actactttaa tccgtggcgg cgctatttgc
     5341 cagaaatacg gcttttgctg cccatttttc agcttcgctt ccactaatca gggctgctac
     5401 aggctcactc atcatccagg actattgcag cctgcgggtt aaaggacctg ttactggcgg
     5461 caaccatcat cattgagctt ttgggtggtg actggtcgcg gatcctgtaa gccgtcattt
     5521 ttcagactga attcaaaatt tataacctgt cgcattgccg ggtgccctgc gggatatagc
     5581 cagtgcctca ttgccgtcag cgtttcccga aaaaaaaggt gacagccggt aagcgacacg
     5641 ttgtccactc gtccatcgtc cagaatatca aacgtggcgg tcacctgacc ttcgatgcgt
     5701 tttgctgcgg cctgtaccgg gtaggttggc tgcagtgtgg ttacgggttt tagtggtatc
     5761 gcatgtaccg gagtgaggaa catggccgcc agcactgtca gagtaaaagc tttcatggtc
     5821 tgaaatccag aggggcgttt ttgaatattt gccaggtatt ttcatccggt gccggcggaa
     5881 ttttggcgtg ggtaacggct gatattgctg ctttacagag tttcggatct cctccttcag
     5941 caatggcgct gttcagagtg ccatcacgtt gtatggatat ccgcacggtg cacatttttc
     6001 ccgcataggc ctctggttca aacatctgca tttcaacagc ggtcttgatg gccgcagcgt
     6061 agctactgat tttagatgac ttatccagag tataaatttt cggcggctgg gtggtggaat
     6121 gcttacaggc cgtcaggagt aaggggctgc acaatgccag caggataaag ggtattaaac
     6181 gcatcgggtt gatgacctgt cagtaaaaaa tgttacatgt atatttgtca ctctttacgg
     6241 ccacgttcat ttacataaac acggcttatc gtatcgtttt tacaccaaac ggcccgccat
     6301 gcagttgcgt gcataggcat gcagcgggtt acaatttacc gggcgcgttt ccggcggttt
     6361 tccgggtggt ttgttgcctg ttttatcccg tagccgccgg aaacgccctc agaccgtctg
     6421 agcggtgcgg gcaatgcgac gttatggtaa atacccctta ccggcggcgc tgtggctgcc
     6481 aggtgaaggt ggtttccgta gcctcgtatt tctcccgacg catttcctgc gcccgcctgg
     6541 cttcacgttc gtcctgaatg gcacgaagca ccctttcccg cacctgttgt tttaccgcct
     6601 ttggcagcgt tttgttccct ttcaggccca tggccagtgc ctgagcgaca aaggcgtccg
     6661 ccaggcgctt ttcttcagcg ggagtcagtt tcaggtgcgt catagtggcc tccgggcgtc
     6721 tcaggtttcc cgaacagttt aaccgcaggt cctctcctca tgccagttag ctttcagatg
     6781 tgtctatacc atctgaaatg taagtgtgat gaatgtctta aataataaaa tttgttgcca
     6841 tgagaaattt tcgggaaaag acgcgatctg cttacaggtg gctccgggcg cttttttgtg
     6901 cctgaagatg agaactaatc tcatatattg acacctgaaa acgggaggag taaggtaata
     6961 attatactgt gtatatgtac agtatgttgg ctttaattta ttgttttaaa tgtcaaagag
     7021 gaaaacggta tgagtggtgg agacggtcga ggtccgggta attcaggtct gggacataat
     7081 ggtggtcagg ccagtgggaa tgtgaacggt acgtctggta aaggtggccc ttcatcaggt
     7141 ggtggtacgg atccaaacag cgggccgggc tggggtacga cgcatacgcc gaatggagat
     7201 attcataact ataatccggg ggagtttggt aacggcggga gtaaacccgg tggtaatggt
     7261 ggtaacagcg gcaatcatag cggtagctct ggtggcggac agtcttcggc cactgcgatg
     7321 gccttcggtc tgcctgccct ggctactccg ggggctgaag gactggcttt atcagtttcc
     7381 ggcgatgcgt tgtcggccgc cgttgctgat gtgctggctg ccctgaaggg gccgtttaag
     7441 tttggtctgt gggggattgc gatctacggt gtgctgcctt ctgagattgc aaaagatgat
     7501 ccgaaaatga tgtcaaaaat tatgacgtca ttaccggccg atgcggtgac ggagactccg
     7561 gcaagtactt taccactgga ccaggcgacg gttcgtgtca gacaacgggt tgtggatgtg
     7621 gtgaaggatg agcggcagca tattgcggtt gtcgcaggtc ggccaatgag tgtccctgtg
     7681 gtggatgcga aaccgacaaa acgtccgggg gtattcagtg tgtcgattcc gggtctcccg
     7741 tctctgcagg tgagcgtacc gaaaggtgtt ccgacagcga aagccccgcc aaaaggcatt
     7801 gttgctgaaa aaggtgattc acgtccggct ggttttacag ccggtggtaa ctcccgtgag
     7861 gccgttattc gtttcccgaa agagaccgga cagaagccgg tttatgtgtc ggtgacagat
     7921 gttcttaccc cggcacaggt aaaacagcgt caggaggaag aaaagcgtcg ccagcaggca
     7981 tgggacgccg ctcatccgga agaggggctg aaaagagact atgataaagc gaaagctgag
     8041 ctggatgccg aagataaaaa tattgcgacc ttaaacagcc gcattgcatc gacagagaag
     8101 gcgctccccg gtgcaagggc tgctgttcag gaagccgata aaaaggtaaa agaggcagag
     8161 gcaaataaag atgattttgt gacttataac ccgcctcatg aatatggctc cgggtggcag
     8221 gatcaggttc gctatcttga taaggatatt cagaatcaga atgagaaatt aaaagcggct
     8281 caggcatctt taaacgcaat gaatgaatcc ttatccagag ataaggctgc acttaccggg
     8341 gcgatggaga gccggaaaca aaaggagaaa aaagcgaagg atgcagaaaa taagttaaat
     8401 gaggaaaaga acaagcctcg caaggggact aaagactacg gccatgatta tcatccagta
     8461 cctaaaacag aagacataaa gggacttgga gagttaaagc gaggtgatcc taaaacacct
     8521 aaacaaggtg gtggtggtaa gcgggcccga tggtatggcg ataaaaaacg taaaatttat
     8581 gaatgggatt cccagcacgg tgagcttgaa gggtatcgtg ccagtgatgg cgaacaccta
     8641 ggcgcatttg atcccaaaac gggtaagcag gttaaagggc cagatccaaa acggaacatt
     8701 aaaaaatatc tttgagaggt aaatatgggg cttaaattac gtttgacatg gtttgataag
     8761 aaaactgaag agtttaaagg ggaagagtat tctaaagact ttggtgacga tggttctgtc
     8821 attgaaagct tgggaatgcc tttaaaggat aatattaata atggtgagtt tgatttgaag
     8881 aaagaatggg ttcctttact acagccattt tttaaaaatc aaataaaacc tgatgtggat
     8941 aattgttata tagcttttga ttatcgggac ggtaactggt aacaaaaaat tatcaaggat
     9001 gatttgaaat actgacttcc ggtgttcagg gatgaatgcc ggagtcttta aatacaggga
     9061 aagtatatga aaaaggtaaa gactattttt ctttttatat tgattgttgc tggctttctt
     9121 ctggccgcat gtcaggcaaa ttatatccgg gatgttcagg gcggaacggt ggcaccatcg
     9181 tcctcctctg aactgacggg gatcgcggtt cagtagaaaa gatcaaagga tcttcttgag
     9241 atcctttttt tctgcgcgta atcttttgcc ctgtaaacga aaaaaccacc tggggaggtg
     9301 gtttgatcga aggttaagtc agttggggaa ctgcttaacc gtggtaactg gctttcgcag
     9361 agcacagcaa ccaaatctgt ccttccagtg tagccggact ttggcgcaca cttcaagagc
     9421 aaccgcgtgt ttagctaaac aaatcctctg cgaactccca gttaccaatg gctgctgcca
     9481 gtggcgtttt gcgtgctttc ccgggttgga ctcaagtgaa cagttaccgg ataaggcgca
     9541 gcagtcgggc tgaacgggga gttcttgctt acagcccagc ttggagcgaa cgacctacac
     9601 cgagccgaga taccagtgtg tgagctatga gaaagcgcca cacttcccgc aagggagaac
     9661 ggcggacagg tatccggtaa acggcagggt cggaacagga gagcgcaaga gggagcgacc
     9721 taccggaaac ggtggggatc tttaagtcct gtcgggtttc gcccgtactg tcagattcat
     9781 ggttgagcct cacggctccc acagatgcac cgaaaaagcg tctgtttatg tgaactctgg
     9841 caggagggcg gagcctatgg aaaaacgcca ccggcgcggc cctgccgttt tgcctcacat
     9901 gttagtcccc tgcttatcca cggaatctgt gggtaacttt gtatgtgtcc gcagcgcccg
     9961 ccgcagtctc acgcccggag cgtagcgacc gagtgagcga ggaggcgcaa tttccccggc
    10021 acctgcaaga cggtcaccgg acgcgctgat gtgggatatt
//
