LOCUS       NZ_CP053780             4167 bp    DNA     linear   CON 09-JAN-2024
DEFINITION  Klebsiella pneumoniae strain BP3636 plasmid punnamed_2_VBP3636.
ACCESSION   NZ_CP053780
VERSION     NZ_CP053780.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14402341
            Assembly: GCF_011742415.2
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Veeraraghavan,B., Shankar,C., Vasudevan,K. and Jacob,J.
  TITLE     Klebsiella pneumoniae VBP3636
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   Veeraraghavan,B., Shankar,C., Vasudevan,K. and Jacob,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
REFERENCE   3  (bases 1 to 4167)
  AUTHORS   Veeraraghavan,B., Shankar,C., Vasudevan,K. and Jacob,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2020) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: Unicycler v. 4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 106.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/09/2024 04:03:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,683
            CDSs (total)                      :: 5,561
            Genes (coding)                    :: 5,366
            CDSs (with protein)               :: 5,366
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 94 of 195
            Pseudo Genes (incomplete)         :: 99 of 195
            Pseudo Genes (internal stop)      :: 35 of 195
            Pseudo Genes (multiple problems)  :: 30 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP053780.1.
            Bacteria and source DNA available from Department of Clinical
            Microbiology, Christian Medical College, Vellore, India.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="BP3636"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="punnamed_2_VBP3636"
                     /geo_loc_name="India"
                     /lat_lon="12.9246 N 79.1348 E"
                     /collection_date="2019"
                     /collected_by="Dept of Microbiology, CMC Vellore"
     gene            complement(<1..410)
                     /locus_tag="HBJ33_RS28235"
                     /old_locus_tag="HBJ33_028790"
     CDS             complement(<1..410)
                     /locus_tag="HBJ33_RS28235"
                     /old_locus_tag="HBJ33_028790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WP_179108549.1"
                     /translation="MIKEISLKNFKCFNELYLKQLKTLNIIAGKNNYGKTSILDAIFCF
                     YDVKNPAVLLNIQAFRKEMAEINKNKPFWVSYFHDMDTSQKMSIVIRDERSEVTQTYET
                     ETNQRLESSLSLNVDTVLSNQNIPRQTIGSQAT"
                     /name="AAA family ATPase"
     gene            complement(755..868)
                     /locus_tag="HBJ33_RS28290"
                     /old_locus_tag="HBJ33_028795"
                     /pseudo=""
     CDS             complement(755..868)
                     /locus_tag="HBJ33_RS28290"
                     /old_locus_tag="HBJ33_028795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            1284..1475
                     /locus_tag="HBJ33_RS28240"
                     /old_locus_tag="HBJ33_028805"
     CDS             1284..1475
                     /locus_tag="HBJ33_RS28240"
                     /old_locus_tag="HBJ33_028805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1475..1876
                     /locus_tag="HBJ33_RS28245"
                     /old_locus_tag="HBJ33_028810"
     CDS             1475..1876
                     /locus_tag="HBJ33_RS28245"
                     /old_locus_tag="HBJ33_028810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_012579088.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2830..3462)
                     /locus_tag="HBJ33_RS28250"
                     /old_locus_tag="HBJ33_028815"
     CDS             complement(2830..3462)
                     /locus_tag="HBJ33_RS28250"
                     /old_locus_tag="HBJ33_028815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3226 domain-containing protein"
                     /protein_id="WP_012579086.1"
                     /translation="MKDINNSKKIIFVEGDDDFHFLCNLLEIQGITDVFVEKINGKSKL
                     KQALRAFKNIDSFDEKESIFIILDADTSFSDTERSIIATLRELQISSPSSHNEIAMNGN
                     TKISFFIMPGKDKQGAIEDLIIEHASTRALYEYIIEFFEKIKNNKDDISASDKEYTYPT
                     NEKKAKVQVYLSCNRESDSRIGISMKNGIINTADNCFDEIKDFISKI"
                     /name="DUF3226 domain-containing protein"
     gene            complement(3462..>4167)
                     /locus_tag="HBJ33_RS28255"
                     /old_locus_tag="HBJ33_028820"
     CDS             complement(3462..>4167)
                     /locus_tag="HBJ33_RS28255"
                     /old_locus_tag="HBJ33_028820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405083.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WP_179108550.1"
                     /translation="VNSLKINVTETPNAKNRVSNKLFSMQLKEDGVEIKSEIKNERDGI
                     NYKFKTATIITTSRKINKEATITNVSSLLTQKRKKDILENLKKIDDRIVDIAISAIGNN
                     KEIYLDIGFSELNEISMLGEGISRALSFISSVLVQENSIILIDEIENGIHYSVIKDIIK
                     SLISSAKQNNNQIFATTHSQDVIRAINEIDSKNEDIAYIRLGREKNSLKPTAVQFNMDD
                     FSYSVENDWEVR"
                     /name="AAA family ATPase"
     Typing          complement(756..1163)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.399"
                     /coverage="99.0"
                     /evalue="5.92e-171"
ORIGIN
        1 gtggcctgag aaccgatagt ttgacgaggt atgttttggt tagatagtac ggtgtcaacg
       61 tttagagaca aacttgattc taatctctga tttgtttctg tttcatatgt ttgtgttact
      121 tcagagcgtt catctcgaat gactatggac atcttttgtg atgtgtccat atcatgaaag
      181 taactaaccc aaaatggctt gtttttgttt atttctgcca tttcttttct gaatgcttga
      241 atgtttaaca aaacagccgg gttttttaca tcataaaaac aaaaaatagc atccagtata
      301 gatgttttcc cgtagttgtt tttcccagct atgatattta aagtctttaa ttgctttaga
      361 tatagctcat tgaagcattt gaagttcttt aaacttattt cctttatcat gatagcctct
      421 gttcgtgttt ttgttatatg ccatacgatt tgttagtacc ttaataaaat gtacagcttt
      481 cattgtgatt agctagactt tatgtcaaag attaactttg attgcttgtg ttttaatcga
      541 ttttttttac tttcttcttg agattatctg gcagcagccg taagcttccg gctgtgaacg
      601 aaaaaaaccg ccttggcggg cggttttttc gaaaggttca gtagagttgg cgctctatga
      661 accgaggtaa ctggcttaaa gggagcgcag tcaccaaaac tgttcgttta gtttagcttt
      721 aactggcgta taacttcaag actaactccc taaatcagtt accagtggct gctgccagtg
      781 gcgctttgtc gtgtctttcc gggttggact caagacgata gttaccggat taggcgcagc
      841 agtcggactg aacggggggt tcgtgcatac agtccagctt ggagcgaact gcctacccgg
      901 aactgagtgt caggcgtgga atgagacaaa cgcggccata acagcggaat gacaccggta
      961 aaccgaaagg caggaacagg agagcgcacg agggagccat cagggggaaa cgcctggtat
     1021 ctttatagtc ctgtcgggtt tcgccaccac tgatttgagc gtcagattcc gtgatgctcg
     1081 tcaggggggc ggagcctatg gaaaaacggc cgctgtgcgg cctttccttc tttcctccat
     1141 gcgattactc ggttttctcg agcgtttcta cgcatgctcc tgccgctcgc cgcagccgaa
     1201 cggcaggagc gtagcgactg agtgagcgag gaagcggaaa agaggagatc cctacactga
     1261 ccactgacgc tctgaggcct cttatgacga aacaacagaa aacagcgctg aacatggcta
     1321 aattcatcca gaatcagtct ctgctcctgc tggagaagct taacgagctt gatctcgatg
     1381 tcgaggccga cctttgtgag aaactccacg atgatgcgga gcatcttttc cgtacactgt
     1441 cctcgagact ggatgcatta caggatggta actgatggcc agagtgtcta ttagtgaggc
     1501 tgcccgactg acgggtaaga gtagaacaac actgcacagg ttaattaaag caggtgacct
     1561 gtccacctgt agcggtgagc gcaatgcaaa aatggtagat acttcagagc ttttacgtgt
     1621 ctttggcccc tttgagcaac cgaagggtga acaccattct ggacaggtct ctggacagcg
     1681 tgatacagcc tctgctgacc agagtgaaca ggtgatccgt catctgaaac aggagattga
     1741 acacctgaac accttacttt tgtccaagga ttcgcacatt gacagcctga aacaggccat
     1801 gctccttctt gaaaatcacc aggcaaaatt aactgaatcc ccggcgccat ggtggaaatt
     1861 ctggaaaaaa tcctgacccc gtcatgcttc agttttcctc tccggccatc ccggggagtt
     1921 cctgaacctt cccaaaaatc gtcagatctc tgaacgctcc tcaccactaa aaatacccac
     1981 accccacgca aaattaaaat tttgcagatt ccttatttac aacaatgggt taatgaaaaa
     2041 taaatgtgcg gtgatgtgtt acaaagtgca gatccaagtc gtggacgact ctatggcgaa
     2101 tcgctgacga attgcccacg attcatatgt gaatcacgct ttattatcat attgttatga
     2161 tgaatcgact cttgattcaa ttcatcactt tgggtgtttt ttgctcatgg tgactcttga
     2221 ttcaattcac gattcgaatc gccggtgaat cgtgtggtga gtcgtccggc gacttgccat
     2281 aacgtgattg ccggccgtcg acgaacccga ttcctgaagg tgctgaaaac cggttttccg
     2341 gctgcgccgg caacgcttca cggccacgca ataaattgcg tccccgttca ccgttttcgg
     2401 atggaaagaa cgccggaaac aggacttact ctggctgaat ggcgtgaatt ttcgcgctat
     2461 gcacttgcgt gcatactcat gcatgccgta aaaacagagc ctgcgcgttt ctggcgggtt
     2521 ttcgggtggt ttgttgcctg ttttaccggt tttccgtcag aaacgccctg aggccgtttt
     2581 agcggtgcgt acaattatga ttttatgtta aattgtttta agtgaataga gagaatataa
     2641 cttaagggag catcttgtgc tgggtctttt acattagatc tgcacaatca ttgttttggc
     2701 atctggtgat gtctttagga cgtttgagat tgctgataac taaagtgtat gatgctttct
     2761 atacgttatc cggcgtttaa taacacccta gtgataagcc agcccgtgaa gggctggctt
     2821 gatttggatt tatatttttg aaataaaatc ttttatttca tcaaagcaat tgtctgctgt
     2881 gtttattatt ccatttttca tggatattcc tattcttgag tcggattctc tattgcagct
     2941 aagatagact tgtaccttag cttttttttc gtttgttgga taagtgtact ctttgtcact
     3001 cgctgagatg tcatctttat tatttttaat tttttcgaaa aattctatta tgtattcata
     3061 taacgctcga gtgcttgcgt gttcaattat cagatcttca atggcacctt gcttgtcttt
     3121 tcctggcatt ataaagaaag aaatttttgt attgccattc atagctatct cgttatgcga
     3181 gctgggggag gagatttgta gttctcgcaa tgtggcaatt attgatcttt ctgtgtcaga
     3241 aaatgatgtg tcagcatcta atataataaa aatactttct ttttcatcga aagaatcaat
     3301 gtttttgaat gctctaagtg cttgttttaa ttttgatttt ccatttattt tttcgacaaa
     3361 aacatcggta atgccttgta tttcaagtag gttgcataga aaatgaaaat catcatcacc
     3421 ctcaacgaaa atgatctttt tgctattatt tatgtctttc attatcttac ctcccagtcg
     3481 ttctcaactg agtaggagaa atcatccata ttgaattgta cggctgttgg ttttagggag
     3541 ttcttttctc ttcctagtct aatataagcg atatcttcat ttttgctatc tatttcgttt
     3601 attgctcgta tcacatcttg actatgagtt gttgcaaata tttgattgtt gttttgtttt
     3661 gcagatgaaa ttaatgattt tattatgtct ttaattactg agtaatgtat tccgttctcg
     3721 atttcatcaa taagaataat tgagttttcc tgtaccagca ctgagctgat aaaagataat
     3781 gctcgactga taccctcgcc aagcattgat atttcattta gctcactaaa tccaatgtct
     3841 aaatatattt ccttattgtt acctatcgca gatattgcta tatctacaat tctgtcatct
     3901 atttttttta agttttctag tatgtctttt ttcctttttt gtgttagtag gctagataca
     3961 tttgtaattg ttgcctcttt gtttatcttt ctggatgttg ttattattgt tgctgtctta
     4021 aatttataat taatcccatc cctttcgttt tttatttcgg attttatttc tacgccatct
     4081 tctttcaatt gcatagagaa aagcttattg gaaaccctgt ttttcgcatt gggtgtctcg
     4141 gtcacattta tttttaaaga gtttact
//
