LOCUS       NZ_CP053778             6030 bp    DNA     linear   CON 09-JAN-2024
DEFINITION  Klebsiella pneumoniae strain BP3636 plasmid pColKP3_4.
ACCESSION   NZ_CP053778
VERSION     NZ_CP053778.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14402341
            Assembly: GCF_011742415.2
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 6030)
  AUTHORS   Veeraraghavan,B., Shankar,C., Vasudevan,K. and Jacob,J.
  TITLE     Klebsiella pneumoniae VBP3636
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6030)
  AUTHORS   Veeraraghavan,B., Shankar,C., Vasudevan,K. and Jacob,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
REFERENCE   3  (bases 1 to 6030)
  AUTHORS   Veeraraghavan,B., Shankar,C., Vasudevan,K. and Jacob,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2020) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: Unicycler v. 4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 106.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/09/2024 04:03:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,683
            CDSs (total)                      :: 5,561
            Genes (coding)                    :: 5,366
            CDSs (with protein)               :: 5,366
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 94 of 195
            Pseudo Genes (incomplete)         :: 99 of 195
            Pseudo Genes (internal stop)      :: 35 of 195
            Pseudo Genes (multiple problems)  :: 30 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP053778.1.
            Bacteria and source DNA available from Department of Clinical
            Microbiology, Christian Medical College, Vellore, India.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6030
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="BP3636"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pColKP3_4"
                     /geo_loc_name="India"
                     /lat_lon="12.9246 N 79.1348 E"
                     /collection_date="2019"
                     /collected_by="Dept of Microbiology, CMC Vellore"
     gene            167..439
                     /locus_tag="HBJ33_RS28165"
                     /old_locus_tag="HBJ33_028720"
     CDS             167..439
                     /locus_tag="HBJ33_RS28165"
                     /old_locus_tag="HBJ33_028720"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_228724513.1"
                     /translation="MSKILDMAKDFEQSSKQQANAIEKRLTEEFTRHEKAISGALKSSE
                     EKMSAAIAEHSQKMGWLLLKTWIWMLVSIVLFLSATSGILWYQGN"
                     /name="MbeB family mobilization protein"
     gene            602..874
                     /locus_tag="HBJ33_RS28170"
                     /old_locus_tag="HBJ33_028725"
     CDS             602..874
                     /locus_tag="HBJ33_RS28170"
                     /old_locus_tag="HBJ33_028725"
                     /inference="COORDINATES: protein motif:HMM:NF016769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015060051.1"
                     /translation="MTGLERELLCAFEALQSAYEQQHKAWQESYANLASMYESTKQEAE
                     ISRAAAERLTRQVNGLTQQVDGLSKQLEGLTGLVSKLQKKGNTGS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1212..1565)
                     /locus_tag="HBJ33_RS28175"
                     /old_locus_tag="HBJ33_028730"
     CDS             complement(1212..1565)
                     /locus_tag="HBJ33_RS28175"
                     /old_locus_tag="HBJ33_028730"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_179108544.1"
                     /translation="MILNKLCKLSTEQHSILLMIFLGEYGIFNLSKALEEVSDAFGAKE
                     TAIASAKLLGKGVVNLAHFAATTGLDSVVKQTSNKVLNSESSTEEQREQAKKSAAWADA
                     RIAARNKAENTED"
                     /name="hypothetical protein"
     gene            1835..2632
                     /locus_tag="HBJ33_RS28180"
                     /old_locus_tag="HBJ33_028735"
     CDS             1835..2632
                     /locus_tag="HBJ33_RS28180"
                     /old_locus_tag="HBJ33_028735"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011071128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-232"
                     /protein_id="WP_043907054.1"
                     /translation="MRVLALSAVFLVASIIGMPAVAKEWQENKSWNAHFTEHKSQGVVV
                     LWNENKQQGFTNNLKRANQAFLPASTFKIPNSLIALDLGVVKDEHQVFKWDGQTRDIAA
                     WNRDHDLITAMKYSVVPVYQEFARQIGEARMSKMLHAFDYGNEDISGNVDSFWLDGGIR
                     ISATQQIAFLRKLYHNKLHVSERSQRIVKQAMLTEANGDYIIRAKTGYSTSIEPKIGWW
                     VGWVELDDNVWFFAMNMDMPTSDGLGLRQAITKEVLKQEKIIP"
                     /name="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-232"
     gene            complement(3039..>3191)
                     /locus_tag="HBJ33_RS28185"
                     /old_locus_tag="HBJ33_028740"
                     /pseudo=""
     CDS             complement(3039..>3191)
                     /locus_tag="HBJ33_RS28185"
                     /old_locus_tag="HBJ33_028740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015059985.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="EreA family erythromycin esterase"
                     /name="EreA family erythromycin esterase"
     gene            3567..4515
                     /locus_tag="HBJ33_RS28190"
                     /old_locus_tag="HBJ33_028745"
                     /pseudo=""
     CDS             3567..4515
                     /locus_tag="HBJ33_RS28190"
                     /old_locus_tag="HBJ33_028745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014293673.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            4920..5312
                     /locus_tag="HBJ33_RS28195"
                     /old_locus_tag="HBJ33_028750"
     CDS             4920..5312
                     /locus_tag="HBJ33_RS28195"
                     /old_locus_tag="HBJ33_028750"
                     /inference="COORDINATES: protein motif:HMM:NF037437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="WP_043907050.1"
                     /translation="MSTTQIEKDSRRKAVNGVISSFQLEGEAITDEFKILLEDYINGKI
                     STDEVLAMVSDEPTTTEYDYEITPRTLGLGGGWNLRLLENGEEVGGGVFPLPEHCDFRD
                     EKALQTLLDSLYEDALAEASAWLASR"
                     /name="antitoxin VbhA family protein"
     gene            5294..5632
                     /locus_tag="HBJ33_RS28200"
                     /old_locus_tag="HBJ33_028755"
     CDS             5294..5632
                     /locus_tag="HBJ33_RS28200"
                     /old_locus_tag="HBJ33_028755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_043907051.1"
                     /translation="MAGQPLKRLRSIKIRVSDAELERLRELCPKAQLAEWMREQCLGVA
                     QPQRRTPAPTVDPALLRQLAGMGNNLNQIARRVNSGEWGPLDRLRIIAELSAIGRELEE
                     LHHDHQIP"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5613..>6030
                     /locus_tag="HBJ33_RS28205"
                     /old_locus_tag="HBJ33_028760"
     CDS             5613..>6030
                     /locus_tag="HBJ33_RS28205"
                     /old_locus_tag="HBJ33_028760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_228724514.1"
                     /translation="MIIKFHNRGVGRGSGPVGYLLGRDGNREGATLDRGNPAVIEALID
                     SSPYAKKYTSGVLSFAEADLPRATKDKLMSDFEKALLPGLEGNQYAVLWVEHRDKGRLE
                     LNFVVPNGRSVKPSGRYHAKGFAEGAQGGRSGHYR"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     ARG             1836..2629
                     /name="blaOXA-232"
                     /gene="blaOXA-232"
                     /gene_name="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-232"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          3580..4502
                     /name="rep_cluster_1195"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.886"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          41..927
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.633"
                     /coverage="90.0"
                     /evalue="7.61e-177"
ORIGIN
        1 ccgtacagac gggcttttac gcatggccgg agcagaagtc agagccgcga tgatgtgcgc
       61 aaagccttgc agggtgcggg tttcagcgtg gtacgcgaaa ccaaaaacag cctgagcatt
      121 gccgacccgg acggggggcg gaacatccga ttaaaggggg tgctgtatga gcaagatttt
      181 agacatggcg aaggacttcg agcagagctc gaagcagcag gccaacgcta tcgagaagcg
      241 tctgaccgaa gaattcacga ggcacgagaa agctatctcc ggggcgttga aatcaagcga
      301 ggagaaaatg agcgccgcta ttgccgagca cagccagaaa atgggctggc tgttgctcaa
      361 aacgtggatc tggatgctgg tcagcatcgt cctgttcttg agcgcaacga gcgggatcct
      421 ctggtatcag gggaattgat agcggagcgg caagccactc caagccttgg cagagaaggg
      481 cggcaagcta aagctgaccc agtgcgggaa gaaattggag cacctttgcg tgcagatctc
      541 cgaccccaat ccaaccgttt gggggaagga tacctattgg atcgtgccga aggggtacta
      601 aatgacggga ttggagcggg aattgctctg cgcgttcgag gctttgcaga gcgcatacga
      661 gcagcagcac aaggcatggc aggaaagcta cgcgaacttg gcgagcatgt acgagagcac
      721 caaacaagag gccgagatca gcagggcagc agcagagcga ttgacgaggc aggtgaacgg
      781 cttaacgcag caagtggacg gcttgagcaa gcagctggag ggcttgaccg ggctagtcag
      841 caagttgcaa aagaaaggga acacaggctc ctgaaggcac tggatcgagg ctgggagcgt
      901 ggagacagat cgcgaggctt tgagcgttga cggaacaaaa cggccgtcag ttataaaaaa
      961 aggaaagtgc tatcactttc cttttatcgg cgggaattat cgttgagttc ttctatgcca
     1021 tttttcatag tagtaactgt aatcttcatc tctaccacca gattcaatcc agtcatcata
     1081 ctcagggcta tttttgaaat ctgaatcaga catgctaact ccttgttatt agttggcttc
     1141 tatggaagct agcgcaccgt gaagttatga tcaacgatgt agtccacatc attgtcgaaa
     1201 tgtgcgttca gctagtcttc ggtgttttct gctttatttc tagcggcgat tctcgcgtct
     1261 gcccatgcgg cagacttttt agcctgttct cgttgttctt cggttgaaga ctcgctgttg
     1321 agaactttgt tgctcgtttg tttgaccacc gaatccaaac ctgttgttgc tgcaaaatga
     1381 gctaggttaa caactccctt acctaacaac ttggcactag cgattgcggt ttctttagcc
     1441 ccaaaagcat cagacacctc ctcaagcgct ttagataggt taaaaatccc atactcccct
     1501 aagaatatca tcaataaaat tgagtgttgc tctgtggata acttgcagag tttattaagt
     1561 atcattgcag caaagatgaa atcaatgatt tatcaaaaat gattgaaagg tggttgtaaa
     1621 taatgttaca atgtgtgaga agcagtctaa attcttcgtg aaatagtgat ttttgaagct
     1681 aataaaaaac acacgtggaa tttaggtaat agttaaagct tgtgcctgat tgttgccaca
     1741 attggtcagc agtttgctag ggaatgagag ggcagagaat gataagctta gcacttaatt
     1801 tgtatggata tattgcatta agcaagggga cgttatgcgt gtattagcct tatcggctgt
     1861 gtttttggtg gcatcgatta tcggaatgcc agcggtagca aaggaatggc aagaaaacaa
     1921 aagttggaat gctcacttta ctgaacataa atcacagggc gtagttgtgc tctggaatga
     1981 gaataagcag caaggattta ccaataatct taaacgggcg aaccaagcat ttttacccgc
     2041 atctaccttt aaaattccca atagcttgat cgccctcgat ttgggcgtgg ttaaggatga
     2101 acaccaagtc tttaagtggg atggacagac gcgtgatatc gccgcttgga atcgtgacca
     2161 tgacttaatt accgcgatga agtactcagt tgtgcctgtt tatcaagaat ttgcccgcca
     2221 aattggtgag gcacgtatga gtaaaatgct gcacgccttc gattatggca atgaggatat
     2281 ctcgggcaat gtagacagtt tttggctcga tggtggtatt cgcatttcgg ctacccagca
     2341 aatcgctttt ttacgcaagc tgtatcacaa caagctgcac gtttctgagc gtagtcagcg
     2401 catcgtgaaa caagccatgc tgaccgaagc caatggcgac tatattattc gggctaaaac
     2461 gggatactcg actagtatcg aacctaagat tggctggtgg gttggttggg ttgaacttga
     2521 tgataatgtg tggttttttg cgatgaatat ggatatgccc acatcggatg gtttagggct
     2581 gcgccaagcc atcacaaaag aagtgctcaa acaggagaaa attattccct agaagtggtt
     2641 agcgcgtatt tgtgtgaaat agccgtcata taagctgtaa agatatatgg acaaaatgct
     2701 gatgctcgat gcgcttgtag ttaatggctc atacttaatc ggtcaggcct aatcgactta
     2761 agctccgcgc gttaactttc agcctgagca ttagcatcac attcctaaac gtaaaaagcc
     2821 tagccaattt gctaggcttt tttgttatct caatggccat ggtcactcaa catgggggtg
     2881 tgaataaagg agtggcgatg tcagtaaagg tgaatgccgc tgcgataaag tgcctagccc
     2941 atcaaggatt gtaggctttc ttggctgcta aagcgtttga cgaaaaagtc caagaaggcg
     3001 ctgatattgg ttctccatca ttttcatcta ccggtttact ataggtcaac caggccgtcc
     3061 ttgccggcgc ttgggacgca gacgatggca tcaaatgcct cgctcaaatt cgtttctaca
     3121 gaggcgcttt gggaccgcat tcgctttgct ttcatggggg catctgtcaa tgtcagacat
     3181 gaatcctccg atataggttg atttttgatc tcttcaacac ttggtgttgt ccgactagag
     3241 agacttatat ggatcagtat tcttgcactg acaatggtgc cactcaatcc caaggatcga
     3301 cctagttaga ggcgtagatc aagcaattca aaacagcgca gaagcagaag gaggtggaaa
     3361 tatgttccag ggtgaaaggt tacatgcaac taacgttgct ctctaggatg tgcctgctgt
     3421 aacgtattgt ccagaaaaga caaccccccg ttatcgcttg catcttggcg gagctgcgaa
     3481 aaacgggggc cgtgacgaat cacaagagaa gtctatcaca tggatcatgc caaacaaccc
     3541 cacgccgcca ataagcgcgg tggtgcttga gaaagtgcgc acagcttgag ctgtttcggg
     3601 agcgcctgcc ccggaaaccc tactattccg acgagcttac aacgggccta cggattgcgg
     3661 atgttgcccg cgcccttggt gcgcgttaca tccagccgaa cgggccaacg caccgccact
     3721 ggatcgtttt tgacgtagac catgcagccg ctacgttatc ttgggatgac gtaggcgctc
     3781 cagcccctaa cattaccgtc acgaacaagg ccaacggcca cgcgcatctg atctacggac
     3841 tggatacgcc gatccgcact gccccggatg gaaatgcagc ccctctgcgg tatgccgccg
     3901 ccatcgaggc cgcgctccgg gagaaattag gggcggatat gggctactcc gggctgatct
     3961 gcaagaaccc cctgcatgag cactggctgg tgcaggtatg ggaaccccgg ctctacgatt
     4021 tagccctggc tatccgatta cctcgatctg agctcctaca atggccgcaa atcgcttcct
     4081 gagtacgggc ttgggcgaaa ctgtaccctg ttcgagaaaa cgcgcctgtg ggcctataaa
     4141 gcgatccggc agggctggcc ggattacccc gaatggttgg ccgcttgtgt tgaccgggca
     4201 tcaggatata acgcccagtt cgagcagccg ctccccgcaa acgaagtccg gcacacggcc
     4261 aagtccattg ccaaatggac gcaccagcac ctaagccccg cagggttcag agaggagcag
     4321 gcccggagag gtgcgaaggg cggcaaggta tccaagggag ggggaaggcc gagcaatagc
     4381 ggtaaggaca agtccgatct attgccggag gttctgcggc ttaaggctca ggggtacacc
     4441 aaccgcgata tagcggacga tcttcaaatc tcccccagta cggtgagcgt ttacctaaag
     4501 cgtgatcgtc cgtgatcgaa acaagcctta tcagataata gcccccttta ggggggttcc
     4561 tgcgctcttg tcttttagcc ttaaaaaatc cttttagcgc ggatctttgg cgaggtggac
     4621 aactgcgcct gtccgtccat cgcccccctg ttttttccgg gggtgtgggc ggcagcagtt
     4681 atccacggag ccgctctttc ccttgatctt gctctagggc ttgcagtatc ccgcccatgg
     4741 ttgaataatg tactaagcaa ggctacacag aggcccctct acctgcttcg cggtctcggg
     4801 cctctgtggc cttgttcagg ggtgctacgc ccccccgaaa ccccctgtgg cccccgctcg
     4861 atccgcccct tcgggacggt ctgcgcggtg gcttggctct ggagaatgga cgaggcccta
     4921 tgtcaacaac acagatagag aaagattcga gaaggaaggc agtcaatggc gttatttcta
     4981 gtttccaact agagggggag gccataaccg atgaattcaa aattctactt gaagattata
     5041 tcaatggaaa aatttccacc gatgaggtgt tagctatggt aagtgacgaa cccacaacaa
     5101 cggaatacga ctacgaaatc accccccgca ctctcggtct tggcgggggc tggaatctgc
     5161 gtttgctgga gaacggcgaa gaggtcggcg ggggtgtttt ccccttgccg gagcactgcg
     5221 attttcggga tgaaaaggcg ctccagacgc tactcgatag cctctatgag gacgccttag
     5281 cggaggcctc tgcatggctg gccagccgtt aaagcgcctc cgctctatca agatccgggt
     5341 ctcagatgcg gagcttgagc gcctgcggga gctctgcccc aaggcccaac tggcggagtg
     5401 gatgcgggag caatgtctcg gtgttgcgca accccagcgc cgaacgccgg cgcctaccgt
     5461 agatccggcg ctcttgcggc agttggccgg aatgggcaac aacctgaacc agatcgcccg
     5521 gcgcgttaat tccggggagt gggggccgtt ggatcggctg cgcatcatag cggagctgtc
     5581 cgctataggc cgcgagctgg aggaactgca ccatgatcat caaattccat aaccgtgggg
     5641 taggccgggg ctccgggcct gttggctact tgttgggccg tgacgggaac cgggaaggcg
     5701 caacgctcga ccggggcaac cctgcggtga ttgaagccct gattgattcc agtccctacg
     5761 ccaaaaaata cacctctggc gttctgtcgt ttgccgaggc tgatttaccc agggcgacca
     5821 aggacaagct gatgagcgac tttgaaaagg cgctcctgcc cggactggag ggcaaccagt
     5881 acgctgtttt gtgggtagag caccgcgaca aggggcggct ggagctgaat tttgtagtcc
     5941 cgaacggacg atccgttaaa ccgtcaggcc gttatcacgc caaaggattt gccgaggggg
     6001 cacaaggagg ccgctcaggc cattacagac
//
