LOCUS       NZ_CP053776             8811 bp    DNA     linear   CON 09-JAN-2024
DEFINITION  Klebsiella pneumoniae strain BP3636 plasmid pColKP3_2.
ACCESSION   NZ_CP053776
VERSION     NZ_CP053776.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14402341
            Assembly: GCF_011742415.2
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 8811)
  AUTHORS   Veeraraghavan,B., Shankar,C., Vasudevan,K. and Jacob,J.
  TITLE     Klebsiella pneumoniae VBP3636
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8811)
  AUTHORS   Veeraraghavan,B., Shankar,C., Vasudevan,K. and Jacob,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
REFERENCE   3  (bases 1 to 8811)
  AUTHORS   Veeraraghavan,B., Shankar,C., Vasudevan,K. and Jacob,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2020) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: Unicycler v. 4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 106.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/09/2024 04:03:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,683
            CDSs (total)                      :: 5,561
            Genes (coding)                    :: 5,366
            CDSs (with protein)               :: 5,366
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 94 of 195
            Pseudo Genes (incomplete)         :: 99 of 195
            Pseudo Genes (internal stop)      :: 35 of 195
            Pseudo Genes (multiple problems)  :: 30 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP053776.1.
            Bacteria and source DNA available from Department of Clinical
            Microbiology, Christian Medical College, Vellore, India.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..8811
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="BP3636"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pColKP3_2"
                     /geo_loc_name="India"
                     /lat_lon="12.9246 N 79.1348 E"
                     /collection_date="2019"
                     /collected_by="Dept of Microbiology, CMC Vellore"
     gene            <1..376
                     /locus_tag="HBJ33_RS28060"
                     /old_locus_tag="HBJ33_028615"
     CDS             <1..376
                     /locus_tag="HBJ33_RS28060"
                     /old_locus_tag="HBJ33_028615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_082052676.1"
                     /GO_function="GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class D beta-lactamase"
                     /protein_id="WP_179108539.1"
                     /translation="LDYGNEDISGNVDSFWLDGGIRISATQQIAFLRKLYHNKLHVSER
                     SQRIVKQAMLTEANGDYIIRAKTGYSTSIEPKIGWWVGWVELDDNVWFFAMNMDMPTSD
                     GLGLRQAITKEVLKQEKIIP"
                     /name="class D beta-lactamase"
     gene            complement(780..>931)
                     /locus_tag="HBJ33_RS28065"
                     /old_locus_tag="HBJ33_028620"
                     /pseudo=""
     CDS             complement(780..>931)
                     /locus_tag="HBJ33_RS28065"
                     /old_locus_tag="HBJ33_028620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015059985.1"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="EreA family erythromycin esterase"
                     /name="EreA family erythromycin esterase"
     gene            1304..1666
                     /locus_tag="HBJ33_RS28595"
     CDS             1304..1666
                     /locus_tag="HBJ33_RS28595"
                     /inference="COORDINATES: protein motif:HMM:NF015076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_228724507.1"
                     /translation="MRKCAQLELFRERLPRKPYYSDELTTGLRIADVARALGARYIQPN
                     GPTHRHWIVFDVDHAAATLSWDDVGAPAPNITVTNKANGHAHLIYGLDTPIRTAPDGNA
                     ALCGMPPPSRPRSGRN"
                     /name="replication initiation protein"
     gene            1723..2247
                     /locus_tag="HBJ33_RS28600"
     CDS             1723..2247
                     /locus_tag="HBJ33_RS28600"
                     /inference="COORDINATES: protein motif:HMM:NF020291.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="WP_228724508.1"
                     /translation="MQVWEPRLYDLAWLSDYLDLSSYNGRKSLPEYGLGRNCTLFEKTR
                     LWAYKAIRQGWPDYPEWLAACVDRASGYNAQFEQPLPANEVRHTAKSIAKWTHQHLSPA
                     GFREEQARRGAKGGKVSKGGGRPSNSGKDKSDLLPEVLRLKAQGYTNRDIADDLQISPS
                     TVSVYLKRDRP"
                     /name="primase C-terminal domain-containing protein"
     gene            3212..3289
                     /gene="mobC"
                     /locus_tag="HBJ33_RS28785"
     CDS             3212..3289
                     /gene="mobC"
                     /locus_tag="HBJ33_RS28785"
                     /inference="COORDINATES: protein motif:HMM:NF017524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_323840805.1"
                     /translation="MGNNLNQIARRVNSGEWGPLIGCAS"
                     /name="mobC"
     gene            3332..4786
                     /locus_tag="HBJ33_RS28605"
     CDS             3332..4786
                     /locus_tag="HBJ33_RS28605"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_064996073.1"
                     /translation="MIIKFHNRGVGRGSGPVGYLLGRDGNREGATLDRGNPAVIEALID
                     SSPYAKKYTSGVLSFAEADLPRATKDKLMSDFEKALLPGLEGNQYAVLWVEHRDKGRLE
                     LNFVVPNIELQSGKRLQPYFHKADNPRLDAWRTVVNGELKLHDPDDPLNRQAVITPKDL
                     PRGHKEAAQAITDGLLRMAGAGEIQSRDDVRKALQGAGFSVVRETKNSLSIADPDGGRN
                     IRLKGVLYEQDFRHGEGLRAELEAAGQRYREASDRRIHEARESYLRGVEIKRGENERRY
                     CRAQPENGLAVAQNVDLDAGQHRPVLERNERDPLVSGGIDSGAASHSPSLGREGRQAKA
                     DPVREEIGAPLRADLRPQSNRLGEGYLLDRAEGVLNDGIGAGIALRVRGFAERIRAAAQ
                     GMAGKLRELGEHVREHQTRGRDQQGSSRAIDEAGERLNAASGRLEQAAGGLDRASQQVA
                     KEREHRLLKALDRGWERGDRSRGFER"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(5068..5349)
                     /locus_tag="HBJ33_RS28095"
                     /old_locus_tag="HBJ33_028650"
     CDS             complement(5068..5349)
                     /locus_tag="HBJ33_RS28095"
                     /old_locus_tag="HBJ33_028650"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_043907053.1"
                     /translation="MGFFNLSKALEEVSDAFGAKETAIASAKLLGKGVVNLAHFAATTG
                     LDSVVKQTSNKVLNSESSTEEQREQAKKSAAWADARIAARNKAENTED"
                     /name="hypothetical protein"
     gene            5693..6492
                     /locus_tag="HBJ33_RS28100"
                     /old_locus_tag="HBJ33_028655"
                     /pseudo=""
     CDS             5693..6492
                     /locus_tag="HBJ33_RS28100"
                     /old_locus_tag="HBJ33_028655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011071128.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-232"
                     /name="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-232"
     gene            complement(<6913..>7049)
                     /locus_tag="HBJ33_RS28105"
                     /old_locus_tag="HBJ33_028660"
                     /pseudo=""
     CDS             complement(<6913..>7049)
                     /locus_tag="HBJ33_RS28105"
                     /old_locus_tag="HBJ33_028660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015059985.1"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="EreA family erythromycin esterase"
                     /name="EreA family erythromycin esterase"
     gene            7580..7786
                     /locus_tag="HBJ33_RS28610"
     CDS             7580..7786
                     /locus_tag="HBJ33_RS28610"
                     /inference="COORDINATES: protein motif:HMM:NF015076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_228724510.1"
                     /translation="MVFDVDHAAATLSWDDVGAPAPNITVTNKANGHAHLIYGLDTPIR
                     TAPDGNAALCGMPPPSRPRSGRN"
                     /name="replication initiation protein"
     gene            7852..>8811
                     /locus_tag="HBJ33_RS28110"
                     /old_locus_tag="HBJ33_028665"
     CDS             7852..>8811
                     /locus_tag="HBJ33_RS28110"
                     /old_locus_tag="HBJ33_028665"
                     /inference="COORDINATES: protein motif:HMM:NF020291.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="WP_228724509.1"
                     /translation="MGTPALRFSPLSDYLDLSSYNGRKSLPEYGLGRNCTLFEKTRLWA
                     YKAIRQGWPDYPEWLAACVDRASGYNAQFEQPLPANEVRHTAKSIAKWTHQHLSPAGFR
                     EEQARRGAKGGKVSKGGGRPSNSGKDKSDLLPEVLRLKAQGYTNRDIADDLQISPSTVS
                     VYLKRERSCSGCYAPETFSGSLLDPPLRDGLRGGFLALENGRGPMSTTQIEKDSRRKAV
                     NGVISSFQLEGEAITDEFKIYWVGGGVFLPGTAIFGRVCCAPDATDSLENHPCTSSVSQ
                     AELVSLEKWRRGSAGVFSPCRSTAIFGRVCCAPDATDSL"
                     /name="primase C-terminal domain-containing protein"
     ARG             complement(6803..7038)
                     /name="EreA2"
                     /gene="EreA2"
                     /gene_name="macrolide esterase"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic"
                     /identity="97.06"
                     /coverage="19.12"
     ARG             5927..6492
                     /name="OXA-232"
                     /gene="OXA-232"
                     /gene_name="OXA beta-lactamase; OXA-48-like beta-lactamase"
                     /tool="rgi"
                     /drug_class="carbapenem; penam"
                     /identity="99.47"
                     /coverage="70.94"
     ARG             3..376
                     /name="OXA-232"
                     /gene="OXA-232"
                     /gene_name="OXA beta-lactamase; OXA-48-like beta-lactamase"
                     /tool="rgi"
                     /drug_class="carbapenem; penam"
                     /identity="99.19"
                     /coverage="46.79"
     ARG             5694..5929
                     /name="OXA-163"
                     /gene="OXA-163"
                     /gene_name="OXA beta-lactamase; OXA-48-like beta-lactamase"
                     /tool="rgi"
                     /drug_class="carbapenem; penam"
                     /identity="100.0"
                     /coverage="29.89"
     Typing          1317..2234
                     /name="rep_cluster_1195"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.545"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          7438..8354
                     /name="rep_cluster_1195"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.438"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          3333..4783
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.554"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gctcgattat ggcaatgagg atatctcggg caatgtagac agtttttggc tcgatggtgg
       61 tattcgcatt tcggctaccc agcaaatcgc ttttttacgc aagctgtatc acaacaagct
      121 gcacgtttct gagcgtagtc agcgcatcgt gaaacaagcc atgctgaccg aagccaatgg
      181 cgactatatt attcgggcta aaacgggata ctcgactagt atcgaaccta agattggctg
      241 gtgggttggt tgggttgaac ttgatgataa tgtgtggttt tttgcgatga atatggatat
      301 gcccacatcg gatggtttag ggctgcgcca agccatcaca aaagaagtgc tcaaacagga
      361 gaaaattatt ccctagaagt ggttagcgcg tatttgtgtg aaatagccgt catataagct
      421 gtaaagatat atggacaaaa tgctgatgct cgatgcgctt gtagttaatg gctcatactt
      481 aatcggtcag gcctaatcga cttaagctcc gcgcgttaac tttcagcctg agcattagca
      541 tcacattcct aacgtaaaaa gcctagccaa tttgctaggc ttttttgtta tctcaatggc
      601 catggtcacc aacatggggt gtgaataaag gagtggcgat gtcagtaaag gtgaatgccg
      661 ctgcgataaa gtgcctagcc catcaaggat tgtaggcttt cttggctgct aaagcgtttg
      721 acgaaaaagt ccaagaaggc gctgatattg gttctccatc attttcatct accggtttac
      781 tataggtcaa ccaggccgtc cttgccggcg cttgggacgc agacgatggc atcaaatgcc
      841 cgctcaaatt cgtttctaca gaggcgcttt gggaccgcat tcgctttgct ttcatggggg
      901 catctgtcaa tgtcagacat gaatcctccg atataggttg atttttgatc tcttcaacac
      961 ttggtgttgt ccgactagag agacttatat ggatcagtat tcttgcactg acaatggtgc
     1021 cactcaatcc caaggatcga cctagttaga ggcgtagatc aagcaattca aaacagcgca
     1081 gaagcagaag gaggtggaaa tatgttccgt ggtgaaaggt tacatgcaac taacgttgct
     1141 ctctaggatg tgcctgctgt aacgtattgt ccagaaagac aacccccgtt atcgcttgca
     1201 tcttggcgga gctgcgaaaa cgggggccgt gacgaatcac aagagaagtc tatcacatgg
     1261 atcatgccaa acaaccccac gccgccaata agcgcggtgg tgcttgagaa agtgcgcaca
     1321 gcttgagctg tttcgggagc gcctgccccg gaaaccctac tattccgacg agcttacaac
     1381 gggcctacgg attgcggatg ttgcccgcgc ccttggtgcg cgttacatcc agccgaacgg
     1441 gccaacgcac cgccactgga tcgtttttga cgtagaccat gcagccgcta cgttatcttg
     1501 ggatgacgta ggcgctccag cccctaacat taccgtcacg aacaaggcca acggccacgc
     1561 gcatctgatc tacggactgg atacgccgat ccgcactgcc ccggatggaa atgcagccct
     1621 ctgcggcatg ccgccgccat cgaggccgcg ctccgggaga aattagggcg gatatgggct
     1681 acccgggctg atctgcaaga acccctgcat gagcactggc tggtgcaggt atgggaaccc
     1741 cggctctacg atttagcctg gctatccgat tacctcgatc tgagctccta caatggccgc
     1801 aaatcgcttc ctgagtacgg gcttgggcga aactgtaccc tgttcgagaa aacgcgcctg
     1861 tgggcctata aagcgatccg gcagggctgg ccggattacc ccgaatggtt ggccgcttgt
     1921 gttgaccggg catcaggata taacgcccag ttcgagcagc cgctccccgc aaacgaagtc
     1981 cggcacacgg ccaagtccat tgccaaatgg acgcaccagc acctaagccc cgcagggttc
     2041 agagaggagc aggcccggag aggtgcgaag ggcggcaagg tatccaaggg agggggaagg
     2101 ccgagcaata gcggtaagga caagtccgat ctattgccgg aggttctgcg gcttaaggct
     2161 caggggtaca ccaaccgcga tatagcggac gatcttcaaa tctcccccag tacggtgagc
     2221 gtttacctaa agcgtgatcg tccgtgatcg aaacaagcct tatcagataa tagccccttt
     2281 agggggttcc tgcgctcttg tcttttagcc ttaaaaatcc ttttagcgcg gatttggcga
     2341 ggtggacaac tgcgcctgtc cgtccatcgc cccctgtttt ttttcggggg gtgggcggca
     2401 gcagttatcc acggagccgc tctttccctt gatcttgctc tagggcttgc agtatcccgc
     2461 ccatggttga ataatgtact aagcaaggct acacagaggc cctctacctg cttcgcggtc
     2521 tcgggcctca gggttttgtt caggggtgct acgcccccga aacctctcag gctccctgct
     2581 cgatccgccc cttcgggacg gtctgcgcgg tggcttcttg gctctggaga atggacgagg
     2641 ccctatgtca acaacacaga tagagaaaga ttcgagaagg aaggcagtca atggcgttat
     2701 ttctagtttc caactagagg gggaggccat aaccgatgaa ttcaaaattt acttgaagat
     2761 tatatcaatg gaaaaatttc caccgatgag gtgttagcta tggtaagtga cgaacccaca
     2821 acaacggaat acgactacga aatcaccccc gcactcggtc ttggcggggg ctggaatctg
     2881 cgtttgctgg agaacggcga agaggtcggc gggggtgttt tcccttgccg gagcactgcg
     2941 attttcggga tgaaaaggcg ctccagacgc tactcgatag cctctatgag gacgccttag
     3001 cggaggcctc tgcatggctg gccagccgtt aaagcgcctc cgctctatca agatccgggt
     3061 ctcagatgcg gagtttgagc gcctgcggga gctctgcccc aaggcccaac tggcggagtg
     3121 gatgcgggag caatgtctcg gtgttgcgca accccagcgc cgaacgccgg cgcctaccgt
     3181 agatccggcg ctcttgcggc agttggccgg aatgggcaac aacctgaacc agatcgcccg
     3241 gcgcgttaat tccggggagt gggggccgtt gatcggctgc gcatcatagc ggagctgtcc
     3301 gctataggcc gcgagctgga ggaactgcac catgatcatc aaattccata accgtggggt
     3361 aggccggggc tccgggcctg ttggctactt gttgggccgt gacgggaacc gggaaggcgc
     3421 aacgctcgac cggggcaacc ctgcggtgat tgaagccctg attgattcca gtccctacgc
     3481 caaaaaatac acctctggcg ttctgtcgtt tgccgaggct gatttaccca gggcgaccaa
     3541 ggacaagctg atgagcgact ttgaaaaggc gctcctgccc ggactggagg gcaaccagta
     3601 cgctgttttg tgggtagagc accgcgacaa ggggcggctg gagctgaatt ttgtagtccc
     3661 gaacatcgaa ttgcagagcg ggaagcggct gcaaccctac ttccacaagg ccgacaatcc
     3721 gcgcctcgat gcgtggcgaa ccgtggtaaa cggggagctg aaattgcacg acccggacga
     3781 tccgttaaac cgtcaggccg ttatcacgcc aaaggatttg ccgagggggc acaaggaggc
     3841 cgctcaggcc attacagacg ggcttttacg catggccgga gcaggggaga tccagagccg
     3901 cgatgatgtg cgcaaagcct tgcagggtgc gggtttcagc gtggtacgcg aaaccaaaaa
     3961 cagcctgagc attgccgacc cggacggggg gcggaacatc cgattaaagg gggtgctgta
     4021 tgagcaagat tttagacatg gcgaaggact tcgagcagag ctcgaagcag caggccaacg
     4081 ctatcgagaa gcgtctgacc gaagaattca cgaggcacga gaaagctatc tccggggcgt
     4141 tgaaatcaag cgaggagaaa atgagcgccg ctattgccga gcacagccag aaaatgggct
     4201 ggctgttgct caaaacgtgg atctggatgc tggtcagcat cgtcctgttc ttgagcgcaa
     4261 cgagcgggat cctctggtat cagggggaat tgatagcgga gcggcaagcc actctccaag
     4321 ccttggcaga gaagggcggc aagctaaagc tgacccagtg cgggaagaaa ttggagcacc
     4381 tttgcgtgca gatctccgac cccaatccaa ccgtttgggg gaaggatacc tattggatcg
     4441 tgccgaaggg gtactaaatg acgggattgg agcgggaatt gctctgcgcg ttcgaggctt
     4501 tgcagagcgc atacgagcag cagcacaagg catggcagga aagctacgcg aacttggcga
     4561 gcatgtacga gagcaccaaa caagaggccg agatcagcag ggcagcagca gagcgattga
     4621 cgaggcaggt gaacggctta acgcagcaag tggacggctt gagcaagcag ctggagggct
     4681 tgaccgggct agtcagcaag ttgcaaaaga aagggaacac aggctcctga aggcactgga
     4741 tcgaggctgg gagcgtggag acagatcgcg aggctttgag cgttgacgga acaaaacggc
     4801 cgtcagttat aaaaaaagga aagtgctatc actttccttt tatcggcggg aattatcgtt
     4861 gagttcttct atgccatttt tcatagtagt aactgtaatc ttcatctcta ccaccagatt
     4921 caatccagtc atcatactca gggctatttt tgaaatctga atcagacatg ctaactcctt
     4981 gttattagtt ggcttctatg gaagctagcg caccgtgaag ttatgatcaa cgatgtagtc
     5041 cacatcattg tcgaaatgtg cgttcagcta gtcttcggtg ttttctgctt tatttctagc
     5101 ggcgattctc gcgtctgccc atgcggcaga ctttttagcc tgttctcgtt gttcttcggt
     5161 tgaagactcg ctgttgagaa ctttgttgct cgtttgtttg accaccgaat ccaaacctgt
     5221 tgttgctgca aaatgagcta ggttaacaac tcccttacct aacaacttgg cactagcgat
     5281 tgcggtttct ttagccccaa aagcatcaga cacctcctca agcgctttag ataggttaaa
     5341 aaatcccata ctccccctaa gaatatcatc aataaaattg agtgttgctc tgtggataac
     5401 ttgcagagtt tattaagtat cattgcagca aagatgaaat caatgattta tcaaaaatga
     5461 ttgaaaggtg gttgtaaata atgttacaat gtgtgagaag cagtctaaat tcttcgtgaa
     5521 atagtgattt ttgaagctaa taaaaaacac acgtggaatt taggtaatag ttaaagcttg
     5581 tgcctgattg ttgccacaat tggtcagcag tttgctaggg aatgagaggg cagagaatga
     5641 taagcttagc acttaatttg tatggatata ttgcattaag caaggggacg ttatgcgtgt
     5701 attagcctta tcggctgtgt ttttggtggc atcgattatc ggaatgccag cggtagcaaa
     5761 ggaatggcaa gaaaacaaaa gttggaatgc tcactttact gaacataaat cacagggcgt
     5821 agttgtgctc tggaatgaga ataagcagca aggatttacc aataatctta aacgggcgaa
     5881 ccaagcattt ttacccgcat ctacctttaa aattccctta atagcttgat cgccctcgat
     5941 ttgggcgtgg ttaaggatga acaccaagtc tttaagtggg atggacagac gcgtgatatc
     6001 gccgcttgga atcgtgacca tgacttaatt accgcgatga agtactcagt tgtgcctgtt
     6061 tatcaagaat ttgcccgcca aattggtgag gcacgtatga gtaaaatgct gcacgccttc
     6121 gattatggca atgaggatat ctcgggcaat gtagacagtt tttggctcga tggtggtatt
     6181 cgcatttcgg ctacccagca aatcgctttt ttacgcaagc tgtatcacaa caagctgcac
     6241 gtttctgagc gtagtcagcg catcgtgaaa caagccatgc tgaccgaagc caatggcgac
     6301 tatattattc gggctaaaac gggatactcg actagtatcg aacctaagat tggctggtgg
     6361 gttggttggg ttgaacttga tgataatgtg tggttttttg cgatgaatat ggatatgccc
     6421 acatcggatg gtttagggct gcgccaagcc atcacaaaag aagtgctcaa acaggagaaa
     6481 attattccct agaagtggtt agcgcgtatt tgtgtgaaat agccgtcata taagctgtaa
     6541 agatatatgg acaaaatgct gatgctcgat gcgcttgtag ttaatggctc atacttaatc
     6601 ggtcaggcct aatcgactta agctccgcgc gttaactttc agcctgagca ttagcatcac
     6661 attcctaaac gtaaaaagcc tagccaattt gctaggcttt tttgttatct caatggccat
     6721 ggtcactcaa catgggggtg tgaataaagg agtggcgatg tcagtaaagg tgaatgccgc
     6781 tgcgataaag tgcctagccc atcaaggatt gtaggctttc ttggctgcta aagcgtttga
     6841 cgaaaaagtc caagaaggcg ctgatattgg ttctccatca ttttcatcta ccggtttact
     6901 ataggtcaac cagccgtcct tgccggcgct tgggacgcag acgatggcat caaatgcctc
     6961 gctcaaattc gtttctacag aggcgctttg ggaccgcatt cgctttgctt tcatggggca
     7021 tctgtcaatg tcagacatga atcctccgat ataggttgat ttttgatctc ttcaacactt
     7081 ggtgttgtcc gactagagag acttatatgg atcagtattc ttgcactgac aatggtgcca
     7141 ctcaatccca aggatcgacc tagttagagg cgtagatcaa gcaattcaaa acagcgcaga
     7201 agcagaagga ggtggaaata tgttccaggg tgaaaggtta catgcaacta acgttgctct
     7261 ctaggatgtg cctgctgtaa cgtattgtcc agaaaagaca accccccgtt atcgcttgca
     7321 tcttggcgga gctgcgaaaa acgggggccg tgacgaatca caagagaagt ctatcacatg
     7381 gatcatgcca aacaacccca cgccgccaat aagcgcggtg gtgcttgaga aagtgcgcac
     7441 agcttgagct gtttcgggag cgcctgcccc ggaaacccta ctattccgac gagcttacaa
     7501 cgggcctacg gattgcggat gttgcccgcg cccttggtgc gcgttacatc cagccgaacg
     7561 ggccaacgca ccgccactga tcgtttttga cgtagaccat gcagccgcta cgttatcttg
     7621 ggatgacgta ggcgctccag cccctaacat taccgtcacg aacaaggcca acggccacgc
     7681 gcatctgatc tacggactgg atacgccgat ccgcactgcc ccggatggaa atgcagccct
     7741 ctgcggtatg ccgccgccat cgaggccgcg ctccgggaga aattaggggc ggatatgggc
     7801 tacccgggct gatctgcaag aacccctgca tgagcactgg ctggtgcagg tatgggaacc
     7861 ccggctctac gatttagccc gctatccgat tacctcgatc tgagctccta caatggccgc
     7921 aaatcgcttc ctgagtacgg gcttgggcga aactgtaccc tgttcgagaa aacgcgcctg
     7981 tgggcctata aagcgatccg gcagggctgg ccggattacc ccgaatggtt ggccgcttgt
     8041 gttgaccggg catcaggata taacgcccag ttcgagcagc cgctccccgc aaacgaagtc
     8101 cggcacacgg ccaagtccat tgccaaatgg acgcaccagc acctaagccc cgcagggttc
     8161 agagaggagc aggcccggag aggtgcgaag ggcggcaagg tatccaaggg agggggaagg
     8221 ccgagcaata gcggtaagga caagtccgat ctattgccgg aggttctgcg gcttaaggct
     8281 caggggtaca ccaaccgcga tatagcggac gatcttcaaa tctcccccag tacggtgagc
     8341 gtttacctaa agcgtgagag gtcttgttca gggtgctacg cccccgaaac tttcagtggc
     8401 tccctgctcg atccgcccct tcgggacggt ctgcgcggtg gcttcttggc tctggagaat
     8461 ggacgaggcc ctatgtcaac aacacagata gagaaagatt cgagaaggaa ggcagtcaat
     8521 ggcgttattt ctagtttcca actagagggg gaggccataa ccgatgaatt caaaatttac
     8581 tgggtcggcg ggggtgtttt cctgccgggc actgcgattt tcggacgagt gtgctgcgct
     8641 ccagacgcta ctgatagcct cgaaaatcac ccctgcacct cctcggtctc gcaagctgaa
     8701 ctggtgtcgt tggagaaatg gcgaagaggg tcggcggggg tgttttcccc ttgccggagc
     8761 actgcgattt tcggacgagt gtgctgcgct ccagacgcta ctgatagcct c
//
