LOCUS       NZ_CP053775             9730 bp    DNA     circular CON 09-JAN-2024
DEFINITION  Klebsiella pneumoniae strain BP3636 plasmid pColRNAI, complete
            sequence.
ACCESSION   NZ_CP053775
VERSION     NZ_CP053775.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14402341
            Assembly: GCF_011742415.2
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9730)
  AUTHORS   Veeraraghavan,B., Shankar,C., Vasudevan,K. and Jacob,J.
  TITLE     Klebsiella pneumoniae VBP3636
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9730)
  AUTHORS   Veeraraghavan,B., Shankar,C., Vasudevan,K. and Jacob,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
REFERENCE   3  (bases 1 to 9730)
  AUTHORS   Veeraraghavan,B., Shankar,C., Vasudevan,K. and Jacob,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2020) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: Unicycler v. 4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 106.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/09/2024 04:03:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,683
            CDSs (total)                      :: 5,561
            Genes (coding)                    :: 5,366
            CDSs (with protein)               :: 5,366
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 94 of 195
            Pseudo Genes (incomplete)         :: 99 of 195
            Pseudo Genes (internal stop)      :: 35 of 195
            Pseudo Genes (multiple problems)  :: 30 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP053775.1.
            Bacteria and source DNA available from Department of Clinical
            Microbiology, Christian Medical College, Vellore, India.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9730
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="BP3636"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pColRNAI"
                     /geo_loc_name="India"
                     /lat_lon="12.9246 N 79.1348 E"
                     /collection_date="2019"
                     /collected_by="Dept of Microbiology, CMC Vellore"
     gene            complement(join(9329..9730,1..330))
                     /gene="mobC"
                     /locus_tag="HBJ33_RS28015"
                     /old_locus_tag="HBJ33_028570"
     CDS             complement(join(9329..9730,1..330))
                     /gene="mobC"
                     /locus_tag="HBJ33_RS28015"
                     /old_locus_tag="HBJ33_028570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071653262.1"
                     /translation="MALERYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLAMEARGASGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(330..2291)
                     /locus_tag="HBJ33_RS28020"
                     /old_locus_tag="HBJ33_028575"
     CDS             complement(330..2291)
                     /locus_tag="HBJ33_RS28020"
                     /old_locus_tag="HBJ33_028575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_063938523.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNNRTAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIVWCAGGLV
                     AGILLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSVMGTNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEY
                     QPEAGRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(3877..6066)
                     /locus_tag="HBJ33_RS28025"
                     /old_locus_tag="HBJ33_028580"
     CDS             complement(3877..6066)
                     /locus_tag="HBJ33_RS28025"
                     /old_locus_tag="HBJ33_028580"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_function="GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="WP_063938521.1"
                     /translation="MNKYLGLSIVYMMYTSSVMADDSGAVSKDKNISPEETLIVHGTAV
                     SESTTEGTDSYTTQSANTASKMNLSFRDTPQSTTVITRRRMDDVAAKNIIDVVKTAPGL
                     YVSTAEGSGRPNFMSRGFNANVMYEGFSLPWNSYIPSSQTSSAIFDRVEIVRGATGLVQ
                     GAGDPSAAINMIHKRPTRQYQASTNIGAGRWNDYRGGVDVSGPLNDSATLRGRAVVDGE
                     NSETFRDSEKRRNNVIYGIMEADLTDNTLLTIGGYQQYEYNNHWWYDLPISGTGHHLNL
                     PRSTFTGNDWEFERSKSKTAFATLEQKFSNSWKLKFSTLQNWRNADLLGTATYRHKDTR
                     QSMYQQVWGSLREYNSKSYDLSLNGDYALFGRVHQFVAGTTQQKYTDKDTGYTYSPIYI
                     ENVNIFDHGSHSSKKPMKMSGDASYDVTEQKSAYAATHFLLSDNWKILLGGRLDWYEYT
                     SKGTSINTGYKVTRNITKYAGSTYNLDDHHTIYGSYTDIFNPQSSTDLNGKVIKPIVGK
                     NYETGIKGEYFNGLLNASLAVFQVDQENRARLLPDQTGCVTNGNPQCYEASGKVRTKGI
                     DFELQGMVTPLWEIGVGYTYADTRYIKDGDRSNEGKRFNTAVSPQNLFKISSMYTFDGK
                     LTNWRAGVNTYWQSKIYANITDTRDGNPVINKQKAYSTTDIIIGYTPMPQLDLQLNITN
                     VFDKVYYTSIGESIGYGTETYGEPRNVKISATYRF"
                     /name="TonB-dependent siderophore receptor"
     gene            complement(6063..7148)
                     /locus_tag="HBJ33_RS28030"
                     /old_locus_tag="HBJ33_028585"
     CDS             complement(6063..7148)
                     /locus_tag="HBJ33_RS28030"
                     /old_locus_tag="HBJ33_028585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006327147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="WP_063938519.1"
                     /translation="MAITIKSAVNVRSRVLIYSLLLFTGSAYAADIAESTFLTRDIGNG
                     LYEMSFDGKNNSLWAASSSSFDRDKGHGIVYQLEPSTLKTKSAVKIARLGFATALDEEH
                     ERLYIGNTLDGSVTVIDTLTGKELSVIPLTEGQDEAHFRPTREMVIDKKNQRLYVSSAA
                     DKGALWVIDMAVGKKIKTIDGLGHAVTGIALNKNGDNIYLVNGDGEIINLDASSYEIKK
                     RFVVEPEKKHFFLNVSLDEKNGRAFITDPDIPGVLVVDVNSGKILHRIDVINSLAILFN
                     PLRNEIYITHRNAKQVSIVDSNSYYVKTTIKTRLMPNSLSLSHDGENLYVSVKQEKKEM
                     STMKDYILKIELSKIAMEPEE"
                     /name="YncE family protein"
     gene            7525..7788
                     /locus_tag="HBJ33_RS28035"
                     /old_locus_tag="HBJ33_028590"
     CDS             7525..7788
                     /locus_tag="HBJ33_RS28035"
                     /old_locus_tag="HBJ33_028590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938517.1"
                     /translation="MSNSTHYENANFLRELAERLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            7778..8077
                     /locus_tag="HBJ33_RS28040"
                     /old_locus_tag="HBJ33_028595"
     CDS             7778..8077
                     /locus_tag="HBJ33_RS28040"
                     /old_locus_tag="HBJ33_028595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009365129.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_063938515.1"
                     /translation="MPYEVEWKQGAIEDVTALFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHTVR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(8139..8516)
                     /locus_tag="HBJ33_RS28045"
                     /old_locus_tag="HBJ33_028600"
     CDS             complement(8139..8516)
                     /locus_tag="HBJ33_RS28045"
                     /old_locus_tag="HBJ33_028600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938513.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQIETHERDGLRLSDETEKMASDALIAEIKALREEVAGLRQEMQAMRLLEH
                     KPETAETPAGTSRRWWQWGKR"
                     /name="hypothetical protein"
     gene            8585..8764
                     /locus_tag="HBJ33_RS28050"
                     /old_locus_tag="HBJ33_028605"
     CDS             8585..8764
                     /locus_tag="HBJ33_RS28050"
                     /old_locus_tag="HBJ33_028605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_021515244.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGKE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(8792..9322)
                     /locus_tag="HBJ33_RS28055"
                     /old_locus_tag="HBJ33_028610"
     CDS             complement(8792..9322)
                     /locus_tag="HBJ33_RS28055"
                     /old_locus_tag="HBJ33_028610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938511.1"
                     /translation="MALYMVFIEDAEDRDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERDKPAGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQISAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     Typing          3092..3672
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.625"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2..330)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.273"
                     /coverage="100.0"
                     /evalue="4.06e-53"
ORIGIN
        1 cgccgccgac acccttgacg gctgaaacgg aataacgtcc gtcagcggtt cgtcctcgtc
       61 gaacgagaac gccaccccgt gcggcgtcag tccccacacg ctgacctgac ggccgtcaac
      121 cgggtatttc gcccggacca gcagcccttt cgccaccagg ctgtccagga agcggtaaac
      181 cggctgcacc gccgcaatcc ccagccgtcg cgcaaccagc tccgccgtgg aacacagctc
      241 ttcccgcagc cagcgcaggg tcagccgggt tttctccgcg ttccgcgcct gccgcttcgc
      301 gtggctgacg ttgtatcgct ccagtgccat cagtacagcc ccgcaaaatc atcatcaccc
      361 ggccgctgcc gcccggcctc cggctggtac tccgccagcg ggtccgtttt cgccagcacc
      421 gggaagcgct gcagcgggat ttcccggcgt tcaacccgca gcgggctgct gaacgccatc
      481 cgggccacgc ccagcccggt catcaccgca aacccgtcag gcaggtgctg cagggtgttc
      541 acgtcaaaca gcggccgcgc ctccttctga atgaagctgt ccccgctgat atgctccacg
      601 ttccccgcct cccggctggc ccggcggcgc tccacgtcca cccggatttc cccggtctgg
      661 cccgccgccc actgcgcgga ctcctggctg gccgcccggt agaaccagcg gatcggcgtg
      721 ttatccagca ccgtggtttt cacaaactca gcgggcagat cctggccgca ctgcccgaag
      781 tcgcccagcg actggtgcgc gagcagcagg ttgcagtccc gactgcgcag cgtccccagc
      841 gcgttcagca cgtacttcga cagcaggtat ttaatctcat cgagcatgat gctcgcgtgc
      901 ggccacctcc ggaactcatc ccgcgcaatg atgatttgcg cgcagcgggc aaacagcatc
      961 ttctgcaccc ggatcaccgc ctcatcgtcc atcgagccga tgacgtacag gcagccgccg
     1021 ccgttgatta tccccgccac gtcgatgccg ctgtccgtct gcaggacgct caggtcggcc
     1081 acgttctcca gcttggtgat cagccccttc acgtcctttt tcagggcctc cggcagcgcg
     1141 tatgccgccg ccagcacctg ctgaatgttt gccccctgcg ggaactgctc cgcaatcagc
     1201 ttcgccgctt tctgctcgct gatgcggtag tggtccgcca cgtcgccggt ctcactcagg
     1261 ttaaacccgg caaccagcag attcttcagg gcgtactggt cgagatcgcg aaacagattc
     1321 agctgcgccg gtttccccgg ccgcagatcc agcagggtga agtccgggca gtgcgcccgg
     1381 aagacgtgcg gcgcccacgc gtcccctttg gggtcgaagt acaccaccgc gtccccgaaa
     1441 tcgcgtacgc agcgaatgag cgcgttggtg cccatcacgc tcttgccgct cccgggcagg
     1501 ccgaggatct gaatattggt cttgcgccag gtatcacgcg ggaccagcac cggctggccc
     1561 tgctcatcct tacccagaaa caccgcctgc tgtttccggg cctgctctgt ggaaaaatcg
     1621 gccagcgcgt taaccggtac cggctccccg aaatgcaggt ggcggacgtc ggtcatttct
     1681 ccggtccgct ggctgctgcg ccggctctcc tggttcagtt tcgcattcac cgcctcgtca
     1741 gcggccgcaa aatgaccgtc aagcagcaga aatgccagca gccgcgccag aaggatgccg
     1801 gcgacaagcc ctccggcaca ccagacaatc ccgaacaggt tagccggctg cagcaggtaa
     1861 taatctgcgt tcgcgtagag gtccggacgc aggacggttt ctccccagct ctcgataaac
     1921 acggcgacaa ccagcccccc cagaagggcc agaccggcag cgccggtcag ccagtacgcg
     1981 tagttccaga catgccgccg tgccctgacc atccgcccgg gcgaatccag cgaagccagc
     2041 accgaaaatg gcgcagtccg gttattatcg cgcttatagc gatcggcata ctcccgtcgc
     2101 tccttatgac tcagccccac aaaacgcatc cgtccggccc agagttcagc cagaccgaaa
     2161 aacaggccga taaccaggaa agccccggcc agaccgtaat actccctgta cagccagctg
     2221 acccacgggt aaatctgccg ggccagctcc gggatatgcc actgccaggc aagcgaatcc
     2281 gtattaaaca tgcaatctct ctccttcaga cgacgggttt accggcgtgg tgtgcagccc
     2341 ctgctgcaca agccgcggac atgccccgag gcatggcggc ggcggtcacg tccgtggtga
     2401 ttgcattcag ctttcaccac ggaggccagc ctctttccgc cccaagcgga aaaggcgcag
     2461 ccccggagga acaacgagca cgccccagcg tgtgagagga aaaggcggca ccccagtgcg
     2521 gtcttttccg gtgttccggc ccccgtcaga gttcgccata gcgagcggct gaccacccgc
     2581 aaaatcagga gcagctttgc tgcttcgtca ggtgcgttca gcaccgtggg tttttgtggg
     2641 tggtcaggcg tgagcctgac gggggccggg tgaatttctg gcgtggccgg cgtccagccg
     2701 agctgagcca gaaagagggg gaaagaccgt gcgtcggtgg cgtccagcca gcgtaagcag
     2761 gggatttcct cctgaagtca gccacgcccc agcggggatg acctgcattt ctccgcaagg
     2821 agaaaaaagc aacagagata ggttaacgta aaatattcag cagaaaaccg acagacttca
     2881 cctatctgaa tgatttatcg gcagggaagg gaaaggcggt aacacggtgc aatatgtcac
     2941 atccgcgcgt ccggtggccg tctgcgggag gggggatatt gcgcctcctc cctcactcag
     3001 tcgctacgct ccggccgtga gactgcggcg ggcgctgcta actctcgcaa agttacccac
     3061 agattccgtg gataagcagg ggactaacat gtgaggcaaa acggcagggc cgcgccggtg
     3121 gcgtttttcc ataggcttcg ccctcctgcc agaattcaca taaacagacg ctttttcggt
     3181 gcatctgtgg gagccgtgag gctcaaccat gaatctgaca gtacgggcga aacccgacag
     3241 gacttaaaga tccccaccgt ttccggcggg tcgctccctc ttgcgctctc ctgttccgac
     3301 cctgccgttt accggatacc tgttccgcct ttctccctta cgggaagtgt ggcgctttct
     3361 catagctcac acactggtat ctcggctcgg tgtaggtcgt tcgctccaag ctgggctgta
     3421 agcaagaact ccccgttcag cccgactgct gcgccttatc cggtaactgt tcacttgagt
     3481 ccaacccgga aagacacgca aaaacgccac tggcagcagc cattggtaac tgggagttcg
     3541 cagaggattt gtttagctaa acacgcggtt gctcttgaag tgtgcgccaa agtccggcta
     3601 cactggaagg acagatttgg ttgctgtgct ctgcgaaagc cagttaccac ggttaagcag
     3661 ttccccaact gacttaacct tcgatcaaac cacctcccca ggtggttttt tcgtttacag
     3721 ggcaaaagat tacgacgaac catggaggat ctcaagaaga tcctctacat gatttgatta
     3781 accatcaact gaagtggcaa acagggggtt gtacgccgga accacaaaag acggatttat
     3841 aggggcaaat ccgaggagaa gacccaatcc gcatggttag aatctatagg ttgcactaat
     3901 tttaacattt cttggttctc catatgtttc cgtgccatat ccaatcgatt cacctatgga
     3961 tgtataataa accttatcaa agacattagt tatattgagc tgtaaatcca gttgtggcat
     4021 tggagtatag ccaataataa tgtcagttgt tgaataagcc ttttgtttat taataaccgg
     4081 attgccatcc cttgtgtctg ttatgttagc atatatttta ctttgccagt acgtgtttac
     4141 tcctgctcgc cagtttgtta attttccgtc aaaggtgtac atgctcgaaa tcttaaataa
     4201 attttgtggc gacacggcag tattgaatcg cttgccttca ttactacgat ctccatcttt
     4261 tatatatcgc gtatctgcat atgtatatcc caccccaatt tcccagagtg gcgtaaccat
     4321 gccctgaagt tcgaaatcta tcccttttgt gcgtacttta ccgctagcct catagcattg
     4381 aggattgcca ttagtaacac accctgtctg atcgggaagc agcctggctc gattttcctg
     4441 gtctacctga aataccgcca gagaggcatt aagcaagccg ttaaaatact cacccttaat
     4501 acctgtctca taattcttac caacaatagg cttgatcact ttaccattga ggtcggtgct
     4561 tgactgtgga ttgaatatat cggtatagct cccataaatg gtatgatgat cgtcaagatt
     4621 gtatgttgaa cctgcatatt tggtgatatt tctggtaact ttataacctg tattaatgga
     4681 tgtaccttta cttgtatatt cataccagtc aagacggcca cccagcaata ttttccagtt
     4741 atcactaagt agaaaatgag ttgctgcgta ggcgcttttt tgttcagtga cgtcgtagga
     4801 tgcatcacct gacatcttca tgggtttttt cgaagaatgt gaaccatgat caaatatatt
     4861 aacattctca atatatattg gggaatatgt ataacctgta tctttatcgg tgtatttttg
     4921 ctgagttgtt ccagccacga attgatgaac acgaccaaat aaagcatagt ccccattaag
     4981 cgacaggtca tagcttttgg aattatattc cctcagtgat ccccatactt gctgatacat
     5041 actttgtcgg gtatctttat gccggtatgt tgccgtccct aacaggtcag catttctcca
     5101 gttttgcagc gttgaaaatt tcagcttcca gctattactg aacttctgct caagagttgc
     5161 aaatgcagtt ttacttttac ttctttcaaa ctcccagtca ttgcctgtaa acgtcgagcg
     5221 aggaagatta aggtggtgtc cggtaccaga aataggtaag tcataccacc aatgattgtt
     5281 atattcatac tgctgatatc ccccaatcgt aagtagggta ttatcagtca gatctgcctc
     5341 cataattccg taaatgacgt tattcctgcg tttctcacta tccctgaagg tctcactgtt
     5401 ttcgccatcc acaactgccc ttccgcgtaa ggtcgcgctg tcattcagtg gtcctgatac
     5461 atcaacccca ccccggtagt cattccaccg tccggcaccg atgttagtgc tggcctgata
     5521 ttgccgggtc ggacgcttgt gtatcatatt tatcgcggct gaagggtctc cggcaccctg
     5581 aacaagacct gttgccccgc ggactatctc cacccggtca aaaatagctg aactagtttg
     5641 tgaacttgga atgtatgaat tccacggcag gctaaatcct tcatacatga cattagcatt
     5701 aaatcctctg gacataaaat tcggtcttcc ggaaccttct gctgtactga catataatcc
     5761 aggagcggtt ttaaccacat caataatatt ttttgctgct acatcatcca ttcttcgtcg
     5821 cgtaatgact gtagttgact gaggtgtatc ccggaatgac agattcatct ttgatgctgt
     5881 attggctgac tgggttgtgt atgaatctgt tccttctgtt gtgctttcag aaacagcggt
     5941 cccatgaaca attaatgttt cttctggtga aatattttta tctttactaa ctgcgccaga
     6001 atcatcagcc ataacagatg atgtatacat catatatact atgctcagcc ctaaatattt
     6061 attcattctt ctggctccat agcaatttta gataattcga ttttgagtat gtaatccttc
     6121 atagtactca tctctttttt ttcttgtttt acactaacgt acagattttc accgtcatgt
     6181 gataaagaca gactgtttgg catcaggcgg gtttttatcg ttgtttttac gtaataagaa
     6241 ttactgtcca caatgctgac ctgttttgca ttacggtgcg tgatatagat ttcgttccgt
     6301 aaaggattaa ataaaatcgc cagtgaattt ataacatcaa ttctgtgtag tatttttccc
     6361 gagtttacat caacaaccag caccccggga atatccgggt cggtaataaa tgcacgtccg
     6421 tttttttcat ccagacttac attaaggaaa aagtgttttt tctcaggctc tacaacaaac
     6481 cgttttttta tttcatagct actggcatcc aggttaataa tctcaccatc cccgttaacc
     6541 agataaatat tatccccatt tttatttaac gcaattccag taacagcgtg ccccagtcca
     6601 tcaatggttt taattttctt cccgacagcc atgtcaataa cccacagagc ccctttatct
     6661 gcggcgctgg agacataaag acgctgattt tttttgtcaa tgaccatttc tctggtcggt
     6721 ctgaaatgag cctcatcctg tccctcagtt aacggaatta ctgaaagttc ttttccggtt
     6781 agagtatcaa tgaccgtcac tgagccgtcc agagtattgc cgatataaag tcgctcatgt
     6841 tcctcatcca gtgctgtggc aaacccaaga cgtgcgattt taacggcaga ttttgttttc
     6901 agagtggatg gttctaactg ataaacaatt ccgtggccct tatctctgtc gaatgaagac
     6961 gatgatgctg cccagagact attatttttg ccatcaaaag acatttcata cagaccattg
     7021 ccgatatcac gagtcaggaa tgttgattca gctatatcag ccgcatacgc tgaaccagta
     7081 aacagtagta aagagtatat taatactctg ctacggacat taacggcgga tttaatcgtg
     7141 attgccatac ttacatttcc ttacaataaa agaaacaaga atatagcaaa agtgataatg
     7201 attatcacta ccattaatca catgatttac cataacgtcg cattacgcgc accgctcaga
     7261 cggtctgagg gcatttccgg cggctacggg ataaaaccgg caacaaacca cccggaaaac
     7321 cgccggaaac gcgcctgata aattttaacc cgctgcatgg ctatgcacgc aactgcatgt
     7381 cgtgcagttt gacgtaaaat gggtgtcgta agcccttttt taccagcttt cgtcttcatc
     7441 agcctgactg acacgagcat attctttcag accgccccct gctaaactcc ttcatatcga
     7501 cgtatcagcc cgggaggctc cacaatgagc aacagtacac actatgaaaa cgccaatttc
     7561 ctgcgggaac tggccgagag actgccccgc atactgcctg aaggtggccc ggacaaagct
     7621 gcactgttgc agcgcctggc taacgaagaa ctggcccagg cagaatatga agatcaggtt
     7681 cgcgctaaag tgaccgccgc ccgcgctgac acgcgcccgg gcatgaccac ggaacaactc
     7741 cgccagagac tgcatggccg ctatcaggaa ctacgcgatg ccgtatgagg tggaatggaa
     7801 gcagggagcc atcgaggatg tgacagcatt gtttgactac attgcggaaa attcatccct
     7861 gtgggatgcg cagaacgtta ccgatcgagt tctcgctgca gcggacaaac tggccgactt
     7921 cccgcgccta tatgaagtgg actcacgcta tggcgaggac gttcgccgga tcagtctgat
     7981 gggacagcat gtcctctatg atgttgatga ccagaccagg aaagtccggg ttctggccgt
     8041 ggttggccag cgccagaatc cccataccgt tcgctgataa ccggtgatgg gggcgcaatt
     8101 cattgcgtgg ccgtcgccgg ttggacaagc acagcgcgtc agcgttttcc ccactgccac
     8161 catcggcgcg atgttccggc cggtgtctct gctgtctctg gtttgtgttc cagcagtctc
     8221 atggcctgca tttcctgccg cagtccggcc acctcctccc tgagtgcttt tatctccgcg
     8281 atcaacgcgt cggaggccat tttttcagtc tcatccgaca gtctcaaccc gtcccgctcg
     8341 tgagtctcaa tctgtcttaa ttcaccgtac acccggataa gttcgctggt atccaccacc
     8401 cgaccaccat cagcttcagt acggtatgat acgcggcctt tcgccatatc cctatacagg
     8461 gaactccggg ctttccccgt cagttccgtc gcctgtctca ccgtcaccca cgccattgtc
     8521 tcacctccgg ttttgagaca gtatacccga caggaagcac accacacccg gaaggacaga
     8581 aaacatgact aagcaggaaa aaactgccct caatatggct aaatttatta aggaccagtc
     8641 gctgctgtta ctcgagaaac tgaacgagct ggatatggat accccggccg acatgtgcga
     8701 gcggctgcac gaggatgcgg aaacactctg gcaggtgatg cgggataagc tcgggaagga
     8761 ataacagcac cggcatggcc ggtgctgccg tttatttttt gccgaacggc caccaccggc
     8821 gggcgggttt gtcctccggc agttcaggca ccggcacgtt aaaatagcgg ctgttatatt
     8881 cccgcatcag ctccgctttc agcttctcgt tcaccgcgct ttccccgtgc cgctcgctga
     8941 ccacctcaat catcgcgcta atctgctccg gtgagcactt cccggcatca accacctgca
     9001 caccgttctc gatggcgaac cgctccacct tgcgatagag gtaatcacac agcttcattc
     9061 ctgcgggttt gtcccgttcc cattgcggat ttttaccggt gaaaaagtac gtggcttttt
     9121 cgtcgctgtt gagccgctcg tcatacatcc cgatggattt catcatcgcc agatgctggg
     9181 cccggtcgac aaaacagaaa acatacgccc cggtctgcca ggcgagcacc accatatctt
     9241 caagatgctc gctggtcggc atgatgcagc gctcgccgtc ccggtcctcc gcgtcttcaa
     9301 taaataccat gtacagcgcc ataatccctc actccgttgt cataaattta aagcacgcaa
     9361 acagccgctc ccggtccagc tccgccgttt tgcgggcctc gccggtctgt ggattcacga
     9421 tttcatccac cgacaggatc gcccgctgca ccctgacctg cgtcgcccgg tccgggcaga
     9481 tgtaccagat ttcatcgatg ccgttcgctt tgcgattgaa cagatacccg gacaccacct
     9541 cctgatagcg cctgcggctc ttcacggtgc gctccacctc aaacgccacc gttctgccgt
     9601 ccagctccgc cagcgcgtcc ggcaccttca tccccttcag ggccatacgg tgcaggtacc
     9661 gccagccgga ggccccccgg gcctccatgg cgagacgcag ggactgcacg gccagccggt
     9721 gcggcagctg
//
