LOCUS       NZ_CP053774            11154 bp    DNA     circular CON 09-JAN-2024
DEFINITION  Klebsiella pneumoniae strain BP3636 plasmid pColKP3_1, complete
            sequence.
ACCESSION   NZ_CP053774
VERSION     NZ_CP053774.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14402341
            Assembly: GCF_011742415.2
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11154)
  AUTHORS   Veeraraghavan,B., Shankar,C., Vasudevan,K. and Jacob,J.
  TITLE     Klebsiella pneumoniae VBP3636
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11154)
  AUTHORS   Veeraraghavan,B., Shankar,C., Vasudevan,K. and Jacob,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
REFERENCE   3  (bases 1 to 11154)
  AUTHORS   Veeraraghavan,B., Shankar,C., Vasudevan,K. and Jacob,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2020) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: Unicycler v. 4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 106.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/09/2024 04:03:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,683
            CDSs (total)                      :: 5,561
            Genes (coding)                    :: 5,366
            CDSs (with protein)               :: 5,366
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 94 of 195
            Pseudo Genes (incomplete)         :: 99 of 195
            Pseudo Genes (internal stop)      :: 35 of 195
            Pseudo Genes (multiple problems)  :: 30 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP053774.1.
            Bacteria and source DNA available from Department of Clinical
            Microbiology, Christian Medical College, Vellore, India.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11154
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="BP3636"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pColKP3_1"
                     /geo_loc_name="India"
                     /lat_lon="12.9246 N 79.1348 E"
                     /collection_date="2019"
                     /collected_by="Dept of Microbiology, CMC Vellore"
     gene            complement(142..480)
                     /locus_tag="HBJ33_RS27940"
                     /old_locus_tag="HBJ33_028495"
     CDS             complement(142..480)
                     /locus_tag="HBJ33_RS27940"
                     /old_locus_tag="HBJ33_028495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_043907051.1"
                     /translation="MAGQPLKRLRSIKIRVSDAELERLRELCPKAQLAEWMREQCLGVA
                     QPQRRTPAPTVDPALLRQLAGMGNNLNQIARRVNSGEWGPLDRLRIIAELSAIGRELEE
                     LHHDHQIP"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(462..854)
                     /locus_tag="HBJ33_RS27945"
                     /old_locus_tag="HBJ33_028500"
     CDS             complement(462..854)
                     /locus_tag="HBJ33_RS27945"
                     /old_locus_tag="HBJ33_028500"
                     /inference="COORDINATES: protein motif:HMM:NF037437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="WP_043907050.1"
                     /translation="MSTTQIEKDSRRKAVNGVISSFQLEGEAITDEFKILLEDYINGKI
                     STDEVLAMVSDEPTTTEYDYEITPRTLGLGGGWNLRLLENGEEVGGGVFPLPEHCDFRD
                     EKALQTLLDSLYEDALAEASAWLASR"
                     /name="antitoxin VbhA family protein"
     gene            complement(1260..1373)
                     /locus_tag="HBJ33_RS28585"
     CDS             complement(1260..1373)
                     /locus_tag="HBJ33_RS28585"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_179108536.1"
                     /translation="MPEVLRLKAQGYTNRDIADDLQISPSTVSVYLKRDRP"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(1322..2206)
                     /locus_tag="HBJ33_RS27950"
                     /old_locus_tag="HBJ33_028505"
     CDS             complement(1322..2206)
                     /locus_tag="HBJ33_RS27950"
                     /old_locus_tag="HBJ33_028505"
                     /inference="COORDINATES: protein motif:HMM:NF015076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_228724504.1"
                     /translation="MRKCAQLELFRERLPRKPYYSDELTTGLRIADVARALGARYIQPN
                     GPTHRHWIVFDVDHAAATLSWDDVGAPAPNITVTNKANGHAHLIYGLDTPIRTAPDGNA
                     APLRYAAAIEAALREKLGADMGYSGLICKNPLHEHWLVQVWEPRLYDLAWLSDYLDLSS
                     YNGRKSLPEYGLGRNCTLFEKTRLWAYKAIRQGWPDYPEWLAACVDRASGYNAQFEQPL
                     PANEVRHTAKSIAKWTHQHLSPAGFREEQARRGAKGGKVSREGEGRAIAVRTSPIYCRR
                     FCGLRLRGTPTAI"
                     /name="replication initiation protein"
     gene            <2581..2733
                     /locus_tag="HBJ33_RS27955"
                     /old_locus_tag="HBJ33_028510"
                     /pseudo=""
     CDS             <2581..2733
                     /locus_tag="HBJ33_RS27955"
                     /old_locus_tag="HBJ33_028510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015059985.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="EreA family erythromycin esterase"
                     /name="EreA family erythromycin esterase"
     gene            complement(3139..3935)
                     /gene="blaOXA"
                     /locus_tag="HBJ33_RS27960"
                     /old_locus_tag="HBJ33_028515"
                     /pseudo=""
     CDS             complement(3139..3935)
                     /gene="blaOXA"
                     /locus_tag="HBJ33_RS27960"
                     /old_locus_tag="HBJ33_028515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011071128.1"
                     /GO_function="GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-48 family class D beta-lactamase"
                     /name="blaOXA"
     gene            4279..4560
                     /locus_tag="HBJ33_RS27965"
                     /old_locus_tag="HBJ33_028520"
     CDS             4279..4560
                     /locus_tag="HBJ33_RS27965"
                     /old_locus_tag="HBJ33_028520"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_043907053.1"
                     /translation="MGFFNLSKALEEVSDAFGAKETAIASAKLLGKGVVNLAHFAATTG
                     LDSVVKQTSNKVLNSESSTEEQREQAKKSAAWADARIAARNKAENTED"
                     /name="hypothetical protein"
     gene            complement(4839..5141)
                     /locus_tag="HBJ33_RS27970"
                     /old_locus_tag="HBJ33_028525"
     CDS             complement(4839..5141)
                     /locus_tag="HBJ33_RS27970"
                     /old_locus_tag="HBJ33_028525"
                     /inference="COORDINATES: protein motif:HMM:NF016769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD/MobD family mobilization/exclusion protein"
                     /protein_id="WP_228724505.1"
                     /translation="MLCAFEALQSAYEQQHKAWQESYANLASMYESTKQEAEISRAAAG
                     RLTRQVNGNAASGRQQAAGGLDRASQQVAKEREHRLLKALDRGWERGDRSRGFER"
                     /name="MbeD/MobD family mobilization/exclusion protein"
     gene            complement(5158..5457)
                     /locus_tag="HBJ33_RS28590"
     CDS             complement(5158..5457)
                     /locus_tag="HBJ33_RS28590"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_228724506.1"
                     /translation="MSAAIAEHSQKMGWLLLKTWIWMLVSIVLFLSATRQGSSGIRGID
                     SGAASHSQALAEKGGKLKLTQCGKKLEHLCVQISDPNPTVWGKDTYWIVPKGTK"
                     /name="hypothetical protein"
     gene            complement(<5414..6287)
                     /locus_tag="HBJ33_RS27975"
                     /old_locus_tag="HBJ33_028530"
                     /pseudo=""
     CDS             complement(<5414..6287)
                     /locus_tag="HBJ33_RS27975"
                     /old_locus_tag="HBJ33_028530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000635827.1"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(6268..6606)
                     /locus_tag="HBJ33_RS27980"
                     /old_locus_tag="HBJ33_028535"
     CDS             complement(6268..6606)
                     /locus_tag="HBJ33_RS27980"
                     /old_locus_tag="HBJ33_028535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_179108535.1"
                     /translation="MAGQPLKRLRSIKIRVSDAELERLQELCPKAQLAEWMREQCLGVA
                     QPQRRTPAPTVDPALLRQLAGMGNNLNQIARRVNSGEWGPLDRLRIIAELSAIGRELEE
                     LHHDHQIP"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(<7599..8361)
                     /locus_tag="HBJ33_RS27990"
                     /old_locus_tag="HBJ33_028545"
                     /pseudo=""
     CDS             complement(<7599..8361)
                     /locus_tag="HBJ33_RS27990"
                     /old_locus_tag="HBJ33_028545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002030349.1"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            <8693..8839
                     /locus_tag="HBJ33_RS27995"
                     /old_locus_tag="HBJ33_028550"
                     /pseudo=""
     CDS             <8693..8839
                     /locus_tag="HBJ33_RS27995"
                     /old_locus_tag="HBJ33_028550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015059985.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="EreA family erythromycin esterase"
                     /name="EreA family erythromycin esterase"
     gene            complement(9252..10049)
                     /locus_tag="HBJ33_RS28000"
                     /old_locus_tag="HBJ33_028555"
     CDS             complement(9252..10049)
                     /locus_tag="HBJ33_RS28000"
                     /old_locus_tag="HBJ33_028555"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011071128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-232"
                     /protein_id="WP_043907054.1"
                     /translation="MRVLALSAVFLVASIIGMPAVAKEWQENKSWNAHFTEHKSQGVVV
                     LWNENKQQGFTNNLKRANQAFLPASTFKIPNSLIALDLGVVKDEHQVFKWDGQTRDIAA
                     WNRDHDLITAMKYSVVPVYQEFARQIGEARMSKMLHAFDYGNEDISGNVDSFWLDGGIR
                     ISATQQIAFLRKLYHNKLHVSERSQRIVKQAMLTEANGDYIIRAKTGYSTSIEPKIGWW
                     VGWVELDDNVWFFAMNMDMPTSDGLGLRQAITKEVLKQEKIIP"
                     /name="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-232"
     gene            10389..10670
                     /locus_tag="HBJ33_RS28005"
                     /old_locus_tag="HBJ33_028560"
     CDS             10389..10670
                     /locus_tag="HBJ33_RS28005"
                     /old_locus_tag="HBJ33_028560"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_043907053.1"
                     /translation="MGFFNLSKALEEVSDAFGAKETAIASAKLLGKGVVNLAHFAATTG
                     LDSVVKQTSNKVLNSESSTEEQREQAKKSAAWADARIAARNKAENTED"
                     /name="hypothetical protein"
     gene            10769..10900
                     /locus_tag="HBJ33_RS28690"
     CDS             10769..10900
                     /locus_tag="HBJ33_RS28690"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_267314056.1"
                     /translation="MSDSDFKNARQVRVAFLPCLVLLLVCALQSLERAEQFLPNPVI"
                     /name="hypothetical protein"
     ARG             complement(9256..10049)
                     /name="blaOXA-232"
                     /gene="blaOXA-232"
                     /gene_name="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-232"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(3140..3354)
                     /name="OXA-232"
                     /gene="OXA-232"
                     /gene_name="OXA beta-lactamase; OXA-48-like beta-lactamase"
                     /tool="rgi"
                     /drug_class="carbapenem; penam"
                     /identity="100.0"
                     /coverage="26.79"
     ARG             complement(3478..3935)
                     /name="OXA-232"
                     /gene="OXA-232"
                     /gene_name="OXA beta-lactamase; OXA-48-like beta-lactamase"
                     /tool="rgi"
                     /drug_class="carbapenem; penam"
                     /identity="100.0"
                     /coverage="57.36"
     Typing          complement(1274..2194)
                     /name="rep_cluster_1195"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.867"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          complement(7399..8310)
                     /name="rep_cluster_1195"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.243"
                     /coverage="98.0"
                     /evalue="0.0"
ORIGIN
        1 cagaggtgta ttttggcgta ggactggaat caatcagggc ttcaatcacc gcagggttgc
       61 ctggtcgagc gttgcgcctt ccggttcctg tcacggccca acaagtagcc aacaggcccg
      121 gagccccggc ctaccccacg gttatggaat ttgatgatca tggtgcagtt cctccagctc
      181 gcggcctata gcggacagct ccgctatgat gcgcagccga tccaacggcc cccactcccc
      241 ggaattaacg cgccgggcga tctggttcag gttgttgccc attccggcca actgccgcaa
      301 gagcgccgga tctacggtag gcgccggcgt tcggcgctgg ggttgcgcaa caccgagaca
      361 ttgctcccgc atccactccg ccagttgggc cttggggcag agctcccgca ggcgctcaag
      421 ctccgcatct gagacccgga tcttgataga gcggaggcgc tttaacggct ggccagccat
      481 gcagaggcct ccgctaaggc gtcctcatag aggctatcga gtagcgtctg gagcgccttt
      541 tcatcccgaa aatcgcagtg ctccggcaag gggaaaacac ccccgccgac ctcttcgccg
      601 ttctccagca aacgcagatt ccagcccccg ccaagaccga gagtgcgggg ggtgatttcg
      661 tagtcgtatt ccgttgttgt gggttcgtca cttaccatag ctaacacctc atcggtggaa
      721 atttttccat tgatataatc ttcaagtaga attttgaatt catcggttat ggcctccccc
      781 tctagttgga aactagaaat aacgccattg actgccttcc ttctcgaatc tttctctatc
      841 tgtgttgttg acatagggcc tcgtccattc tccagagcca agaagccacc gcgcagaccg
      901 tcccgaaggg gcggatcgag cgggggccac agggggtttc gggggggcgt agcacccctg
      961 aacaaggcca cagaggcccg agaccgcgaa gcaggtagag gggcctctgt gtagccttgc
     1021 ttagtacatt attcaaccat gggcgggata ctgcaagccc tagagcaaga tcaaggaaag
     1081 agcggctccg tggataactg ctgccgccca caccccccgg aaaaacaggg gggcgatgga
     1141 cggacaggcg cagttgtcca cctcgccaaa gatccgcgct aaaaggattt ttaaggctaa
     1201 aagacaagag cgcaggaacc cccctaaagg gggctattat ctgataaggc ttgtttcgat
     1261 cacggacgat cacgctttag gtaaacgctc accgtactgg gggagatttg aagatcgtcc
     1321 gctatatcgc ggttggtgta cccctgagcc ttaagccgca gaacctccgg caatagatcg
     1381 gacttgtcct taccgctatt gctcggcctt ccccctccct tgataccttg ccgcccttcg
     1441 cacctctccg ggcctgctcc tctctgaacc ctgcggggct taggtgctgg tgcgtccatt
     1501 tggcaatgga cttggccgtg tgccggactt cgtttgcggg gagcggctgc tcgaactggg
     1561 cgttatatcc tgatgcccgg tcaacacaag cggccaacca ttcggggtaa tccggccagc
     1621 cctgccggat cgctttatag gcccacaggc gcgttttctc gaacagggta cagtttcgcc
     1681 caagcccgta ctcaggaagc gatttgcggc cattgtagga gctcagatcg aggtaatcgg
     1741 atagccaggc taaatcgtag agccggggtt cccatacctg caccagccag tgctcatgca
     1801 gggggttctt gcagatcagc ccggagtagc ccatatccgc ccctaatttc tcccggagcg
     1861 cggcctcgat ggcggcggca taccgcagag gggctgcatt tccatccggg gcagtgcgga
     1921 tcggcgtatc cagtccgtag atcagatgcg cgtggccgtt ggccttgttc gtgacggtaa
     1981 tgttaggggc tggagcgcct acgtcatccc aagataacgt agcggctgca tggtctacgt
     2041 caaaaacgat ccagtggcgg tgcgttggcc cgttcggctg gatgtaacgc gcaccaaggg
     2101 cgcgggcaac atccgcaatc cgtaggcccg ttgtaagctc gtcggaatag tagggtttcc
     2161 ggggcaggcg ctcccgaaac agctcaagct gtgcgcactt tctcaagcac caccgcgctt
     2221 attggcggcg tggggttgtt tggcatgatc catgtgatag acttctcttg tgattcgtca
     2281 cggcccccgt ttttcgcagc tccgccaaga tgcaagcgat aacggggggt tgtcttttct
     2341 ggacaatacg ttacagcagg cacatcctag agagcaacgt tagttgcatg taacctttca
     2401 ccctcgaaca tatttccacc tccttctgct tctgcgctgt tttgaattgc ttgatctacg
     2461 cctctaacta ggtcgatcct tgggattgag tggcaccatt gtcagtgcaa gaatactgat
     2521 ccatataagt cttctagtcg gacaacacca agtgttgaag agatcaaaaa tcaacctata
     2581 tcggaggatt catgtctgac attgacagat gcccccatga aagcaaagcg aatgcggtcc
     2641 caaagcgcct ctgtagaaac gaatttgagc gaggcatttg atgccatcgt ctgcgtccca
     2701 agcgccggca aggacggccc ggttgaccta tagtaaaccg gtagatgaaa atgatggaga
     2761 accaatatca gcgccttctt ggactttttc gtcaaacgct ttagcagcca agaaagccta
     2821 caatccttga tgggctaggc actttatcgc agcggcattc acctttactg acatcgccac
     2881 tcctttattc acacccccat gttgagtgac catggccatt gagataacaa aaagcctagc
     2941 aaattggcta ggctttttac gtttaggaat gtgatgctaa tgctcaggct gaaagttaac
     3001 gcgcggagct taagtcgatt aggcctgacc gattaagtat gagccattaa ctacaagcgc
     3061 atcgagcatc agcattttgt ccatatatct ttacagctta tatgacggct atttcacaca
     3121 aatacgcgct aaccacttct agggaataat tttctcctgt ttgagcactt cttttgtgat
     3181 ggcttggcgc agccctaaac catccgatgt gggcatatcc atattcatcg caaaaaacca
     3241 cacattatca tcaagttcaa cccaaccaac ccaccagcca atcttaggtt cgatactagt
     3301 cgagtatccc gttttagccc gaataatata gtcgccattg gcttcggtca gcatggcttg
     3361 tttcacgatg cgctgactac gctcagaaac gtgcagcttg ttgtgataca gcttgcgtaa
     3421 aaaagcgatt tgctgggtag ccgaaatgcg aataccacca tcgagccaaa aactgtctac
     3481 attgcccgag atatcctcat tgccataatc gaagcgtgca gcattttact catacgtgcc
     3541 tcaccaattt ggcgggcaaa ttcttgataa acaggcacaa ctgagtactt catcgcggta
     3601 attaagtcat ggtcacgatt ccaagcggcg atatcacgcg tctgtccatc ccacttaaag
     3661 acttggtgtt catccttaac cacgcccaaa tcgagggcga tcaagctatt gggaatttta
     3721 aaggtagatg cgggtaaaaa tgcttggttc gcccgtttaa gattattggt aaatccttgc
     3781 tgcttattct cattccagag cacaactacg ccctgtgatt tatgttcagt aaagtgagca
     3841 ttccaacttt tgttttcttg ccattccttt gctaccgctg gcattccgat aatcgatgcc
     3901 accaaaaaca cagccgataa ggctaataca cgcataacgt ccccttgctt aatgcaatat
     3961 atccatacaa attaagtgct aagcttatca ttctctgccc tctcattccc tagcaaactg
     4021 ctgaccaatt gtggcaacaa tcaggcacaa gctttaacta ttacctaaat tccacgtgtg
     4081 ttttttatta gcttcaaaaa tcactatttc acgaagaatt tagactgctt ctcacacatt
     4141 gtaacattat ttacaaccac ctttcaatca tttttgataa atcattgatt tcatctttgc
     4201 tgcaatgata cttaataaac tctgcaagtt atccacagag caacactcaa ttttattgat
     4261 gatattctta gggggagtat gggatttttt aacctatcta aagcgcttga ggaggtgtct
     4321 gatgcttttg gggctaaaga aaccgcaatc gctagtgcca agttgttagg taagggagtt
     4381 gttaacctag ctcattttgc agcaacaaca ggtttggatt cggtggtcaa acaaacgagc
     4441 aacaaagttc tcaacagcga gtcttcaacc gaagaacaac gagaacaggc taaaaagtct
     4501 gccgcatggg cagacgcgag aatcgccgct agaaataaag cagaaaacac cgaagactag
     4561 ctgaacgcac atttcgacaa tgatgtggac tacatcgttg atcataactt cacggtgcgc
     4621 tagcttccat agaagccaac taataacaag gagttagcat gtctgattca gatttcaaaa
     4681 tagccctgag tatgatgact ggattgaatc tggtggtaga gatgaagatt acagttacta
     4741 ctatgaaaat ggcatagaag aactcaacga tattcctgcc gataaaagga aagtgatagc
     4801 actttccttt ttttataact gacggccgtt ttgttccgtc aacgctcaaa gcctcgcgat
     4861 ctgtctccac gctcccagcc tcgatccagt gccttcagga gcctgtgttc cctttctttt
     4921 gcaacttgct gactagcccg gtcaagccct ccagctgctt gctgccgtcc acttgctgcg
     4981 ttgccgttca cctgcctcgt caatcgccct gctgctgccc tgctgatctc ggcctcttgt
     5041 ttggtgctct cgtacatgct cgccaagttc gcgtagcttt cctgccatgc cttgtgctgc
     5101 tgctcgtatg cgctctgcaa agcctcgaac gcgcagagca attcctgccc aatcccgtca
     5161 tttagtaccc ttcggcacga tccaataggt atccttcccc caaacggttg gattggggtc
     5221 ggagatctgc acgcaaaggt gctccaattt cttcccgcac tgggtcagct ttagcttgcc
     5281 gcccttctct gccaaggctt gagagtggct tgccgctccg ctatcaattc ccctgatacc
     5341 agaggatccc tgcctcgttg cgctcaagaa caggacgatg ctgaccagca tccagatcca
     5401 cgttttgagc aacagccagc ccattttctg gctgtgctcg gcaatagcgg cgctcatttt
     5461 ctcctcgctt gatttcaacg cccggagata gctttctcgt gcctcgtgaa ttcttcggtt
     5521 cggtcagacg ctttcgatag cgttggcctg ctgcttcgag ctctgctcga agtccttcgc
     5581 catgtctaaa atcttgctca tacagcaccc cctttaatcg gatgttccgc cccccgtccg
     5641 ggtcggcaat gctcaggctg tttttggttt cgcgtaccac gctgaaaccc gcaccctgca
     5701 aggctttgcg cacatcatcg cggctctgat ctcccctgct ccggccatgc gtaaaagccc
     5761 gtctgtaatg gcctgagcgg cctccttgtg ccccctcggc aaatcctttg gcgtgataac
     5821 ggcctgacgg tttaacggat cgtccgggtc gtgcaatttc agcccccgtt taccacggtt
     5881 cgccacgcat cgaggcgcgg attgtcggcc ttgtggaagt agggttgcag ccgcttcccg
     5941 ctctgcaatt cgatgttcgg gactacaaaa ttcagctcca gccgcccctt gtcgcggtgc
     6001 tctacccaca aaacagcgta ctggttgccc tccagtccgg gcaggagcgc cttttcaaag
     6061 tcgctcatca gcttgtcctt ggtcgccctg ggtaaatcag cctcggcaaa cgacagaacg
     6121 ccagaggtgt attttttggc gtagggactg gaatcaatca gggcttcaat caccgcaggg
     6181 ttgccctggt cgagcgttgc gccttcccgg ttcccgtcac ggcccaacaa gtagccaaca
     6241 ggcccggagc cccggcctac cccacggtta tggaatttga tgatcatggt gcagttcctc
     6301 cagctcgcgg cctatagcgg acagctccgc tatgatgcgc agccgatcca acggccccca
     6361 ctccccggaa ttaacgcgcc gggcgatctg gttcaggttg ttgcccattc cggccaactg
     6421 ccgcaagagc gccggatcta cggtaggcgc cggcgttcgg cgctggggtt gcgcaacacc
     6481 gagacattgc tcccgcatcc actccgccag ttgggccttg gggcagagct cctgcaggcg
     6541 ctcaagctcc gcatctgaga cccggatctt gatagagcgg aggcgcttta acggctggcc
     6601 agccatgcag aggcctccgc taaggcgtcc tcatagaggc tatcgagtag cgtctggagc
     6661 gccttttcat cccgaaaatc gcagtgctcc ggcaagggga aaacaccccg ccgacctctt
     6721 cgccgttctc cagcaaacgc agattccagc ccccgccaag accgagagtg cggggggtga
     6781 tttcgtagtc gtattccgtt gttgtgggtt cgtcacttac catagctaac acctcatcgg
     6841 tggaaatttt tccattgata taatcttcaa gtagaatttt gaattcatcg gttatggcct
     6901 cccctctagt tggaaactag aaataacgcc attgactgcc ttccttctcg aatcttttct
     6961 atctgtgttg ttgacatagg gcctcgtcca ttctccagag ccaagaagcc accgcgcaga
     7021 ccgtcccgaa ggggcggatc gagcgggggc cacagggggt ttcggggggg cgtagcaccc
     7081 ctgaacaagg ccacagaggc ccgagaccgc gaagcaggta gaggggcctc tgtgtagcct
     7141 tgcttagtac attattcaac catgggcggg atactgcaag ccctagagca agatcaaggg
     7201 aaagagcggc tccgtggata actgctgccg cccacacccc ccggaaaaac aggggggcga
     7261 tggacggaca ggcgcagttg tccacctcgc caaagatccg cgctaaaagg attttttaag
     7321 gctaaaagac aagagcgcag gaacccccct aaagggggct attatctgat aaggcttgtt
     7381 tcgatcacgg acgatcacgc tttaggtaaa cgctcaccgt actgggggag atttgaagat
     7441 cgtccgctat atcgcggttg gtgtacccct gagccttaag ccgcagaacc tccggcaata
     7501 gatcggactt gtcctaccgc tattgctcgg ccttcccctc ccttgatacc ttgccgccct
     7561 tcgcaccctc cgggcctgct ccttctgaac cctgcggggc ttaggtgctg gtgcgtccat
     7621 ttggcaatgg acttggccgt gtgccggact tcgttttggg gagcggctgc tcgaactggg
     7681 cgttatatcc tgatgcccgg tcaacacaag cggccaacca ttcggggtaa tccggccagc
     7741 cctgccggat cgctttatag gcccacaggc gcgttttctc gaacagggta cagtttcgcc
     7801 caagcccgta ctcaggaagc gatttgcggc cattgtagga gctcagatcg aggtaatcgg
     7861 atagccagct aaatcgtaga gccgggttcc catacctgca ccagccagtg ctcatgcagg
     7921 gggttcttgc agatcagccc gagtagccca tatccgcccc taatttctcc cggagcgcgg
     7981 ctcgatggcg gcggcatacc gcagaggggc tgcatttcca tccggggcag tgcggatcgg
     8041 cgtatccagt ccgtagatca gatgcgcgtg gccgttggcc ttgttcgtga cggtaatgtt
     8101 aggggctgga gcgcctacgt catcccaaga taacgtagcg gctgcatggt ctacgtcaaa
     8161 aacgatccag tggcggtgcg ttggcccgtt cggctggatg taacgcgcac caagggcgcg
     8221 ggcaacatcc gcaatccgta ggcccgttgt aagctcgtcg gaatagtagg gtttccgggg
     8281 caggcgctcc cgaaacagct caagctgtgc gcacttttca agcaccaccg cgcttattgg
     8341 cggcgtgggg ttgtttggca tgatccatgt gatagacttc tcttgtgatt cgtcacggcc
     8401 ccgtttttcg cagctccgcc aagatgcaag cgataacggg ggttgtcttt tctggacaat
     8461 acgttacagc aggcacatcc tagagagcaa cgttagttgc atgtaacctt tcacccccga
     8521 acatatttcc acctccttct gcttctgcgc tgttttgaat tgcttgatct acgcctctaa
     8581 ctaggtcgat cctgggattg agtggcacca ttgtcagtgc aagaatactg atccatataa
     8641 gtcttctagt cggacaacac caagtgttga agagatcaaa aatcaaccta tatcggagga
     8701 ttcatgtctg acattgacag atgcccccat gaaagcaaag cgaatgcggt cccaaagcgc
     8761 ctctgtagaa acgaatttga gcgaggcatt tgatgccatc gtctgcgtcc caagcgccgg
     8821 caaggacggc ctcggttgac ctatagtaaa ccggtagatg aaaatgatgg agaaccaata
     8881 tcagcgcctt cttgactttt ttcgtcaaac gctttagcag ccaagaaagc ctacaatcct
     8941 tgatgggcta ggcactttat cgcagcggca ttcaccttta ctgacatcgc cactccttta
     9001 ttcacacccc catgttgagt gaccatggcc attgagataa caaaaagcct agcaaattgg
     9061 ctaggctttt tacgtttagg aatgtgatgc taatgctcag gctgaaagtt aacgcgcgga
     9121 gcttaagtcg attaggcctg accgattaag tatgagccat taactacaag cgcatcgagc
     9181 atcagcattt tgtccatata tctttacagc ttatatgacg gctatttcac acaaatacgc
     9241 gctaaccact tctagggaat aattttctcc tgtttgagca cttcttttgt gatggcttgg
     9301 cgcagcccta aaccatccga tgtgggcata tccatattca tcgcaaaaaa ccacacatta
     9361 tcatcaagtt caacccaacc aacccaccag ccaatcttag gttcgatact agtcgagtat
     9421 cccgttttag cccgaataat atagtcgcca ttggcttcgg tcagcatggc ttgtttcacg
     9481 atgcgctgac tacgctcaga aacgtgcagc ttgttgtgat acagcttgcg taaaaaagcg
     9541 atttgctggg tagccgaaat gcgaatacca ccatcgagcc aaaaactgtc tacattgccc
     9601 gagatatcct cattgccata atcgaaggcg tgcagcattt tactcatacg tgcctcacca
     9661 atttggcggg caaattcttg ataaacaggc acaactgagt acttcatcgc ggtaattaag
     9721 tcatggtcac gattccaagc ggcgatatca cgcgtctgtc catcccactt aaagacttgg
     9781 tgttcatcct taaccacgcc caaatcgagg gcgatcaagc tattgggaat tttaaaggta
     9841 gatgcgggta aaaatgcttg gttcgcccgt ttaagattat tggtaaatcc ttgctgctta
     9901 ttctcattcc agagcacaac tacgccctgt gatttatgtt cagtaaagtg agcattccaa
     9961 cttttgtttt cttgccattc ctttgctacc gctggcattc cgataatcga tgccaccaaa
    10021 aacacagccg ataaggctaa tacacgcata acgtcccctt gcttaatgca atatatccat
    10081 acaaattaag tgctgtttat cattctctgc cctctcattc cctagcaaac tgctgaccaa
    10141 ttgtggcaac aatcaggcac aagctttaac tattacctaa attccacgtg tgtttttatt
    10201 agcttcaaaa atcactattt cacgaagaat ttagactgct tctcacacat tgtaacatta
    10261 tttacaacca cctttcaatc atttttgata aatcattgat ttcatctttg ctgcaatgat
    10321 acttaataaa ctctgcaagt tatccacaga gcaacactca attttattga tgatattctt
    10381 aggggagtat gggatttttt aacctatcta aagcgcttga ggaggtgtct gatgcttttg
    10441 gggctaaaga aaccgcaatc gctagtgcca agttgttagg taagggagtt gttaacctag
    10501 ctcattttgc agcaacaaca ggtttggatt cggtggtcaa acaaacgagc aacaaagttc
    10561 tcaacagcga gtcttcaacc gaagaacaac gagaacaggc taaaaagtct gccgcatggg
    10621 cagacgcgag aatcgccgct agaaataaag cagaaaacac cgaagactag ctgaacgcac
    10681 atttcgacaa tgatgtggac tacatcgttg atcataactt cacggtgcgc tagcttccat
    10741 agaagccaac taataacaag gagttagcat gtctgattca gatttcaaaa atgctcgcca
    10801 agttcgcgta gctttcctgc catgccttgt gctgctgctc gtatgcgctc tgcaaagcct
    10861 cgaacgcgca gagcaattcc tgcccaatcc cgtcatttag tacccttcgg cacgatccaa
    10921 tagggtatcc ccccaaacgg ttggattggg gtcggagatc tgcacgcaaa ggtgctcgtg
    10981 ctcgtgaatt tttcggtcag acgcttcgat agcgttattc gctgcttcga gcgctctgct
    11041 cgaagtcctt cgccatgctc caaaatcttg cccatacagc acccctttaa tcgatgttcc
    11101 gcccccgtcc gggtcggcaa tgctcaggct gttttggttt cgcgtaccac gccg
//
