LOCUS       NZ_CP053769             9730 bp    DNA     circular CON 09-JAN-2024
DEFINITION  Klebsiella pneumoniae strain BA10835 plasmid pColRNAI, complete
            sequence.
ACCESSION   NZ_CP053769
VERSION     NZ_CP053769.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14402356
            Assembly: GCF_011742585.2
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9730)
  AUTHORS   Veeraraghavan,B., Shankar,C., Vasudevan,K. and Jacob,J.
  TITLE     Klebsiella pneumoniae VBA10835
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9730)
  AUTHORS   Veeraraghavan,B., Shankar,C., Vasudevan,K. and Jacob,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: Unicycler v. 4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 146.0x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/09/2024 04:30:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,847
            CDSs (total)                      :: 5,724
            Genes (coding)                    :: 5,538
            CDSs (with protein)               :: 5,538
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 85 of 186
            Pseudo Genes (incomplete)         :: 112 of 186
            Pseudo Genes (internal stop)      :: 27 of 186
            Pseudo Genes (multiple problems)  :: 33 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP053769.1.
            Bacteria and source DNA available from Department of Clinical
            Microbiology, Christian Medical College, Vellore, India.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9730
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="BA10835"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pColRNAI"
                     /geo_loc_name="India"
                     /lat_lon="12.9246 N 79.1348 E"
                     /collection_date="2019"
                     /collected_by="Dept of Microbiology, CMC Vellore"
     gene            join(8936..9730,1..1395)
                     /locus_tag="HBJ44_RS29010"
                     /old_locus_tag="HBJ44_029600"
     CDS             join(8936..9730,1..1395)
                     /locus_tag="HBJ44_RS29010"
                     /old_locus_tag="HBJ44_029600"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_function="GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="WP_063938521.1"
                     /translation="MNKYLGLSIVYMMYTSSVMADDSGAVSKDKNISPEETLIVHGTAV
                     SESTTEGTDSYTTQSANTASKMNLSFRDTPQSTTVITRRRMDDVAAKNIIDVVKTAPGL
                     YVSTAEGSGRPNFMSRGFNANVMYEGFSLPWNSYIPSSQTSSAIFDRVEIVRGATGLVQ
                     GAGDPSAAINMIHKRPTRQYQASTNIGAGRWNDYRGGVDVSGPLNDSATLRGRAVVDGE
                     NSETFRDSEKRRNNVIYGIMEADLTDNTLLTIGGYQQYEYNNHWWYDLPISGTGHHLNL
                     PRSTFTGNDWEFERSKSKTAFATLEQKFSNSWKLKFSTLQNWRNADLLGTATYRHKDTR
                     QSMYQQVWGSLREYNSKSYDLSLNGDYALFGRVHQFVAGTTQQKYTDKDTGYTYSPIYI
                     ENVNIFDHGSHSSKKPMKMSGDASYDVTEQKSAYAATHFLLSDNWKILLGGRLDWYEYT
                     SKGTSINTGYKVTRNITKYAGSTYNLDDHHTIYGSYTDIFNPQSSTDLNGKVIKPIVGK
                     NYETGIKGEYFNGLLNASLAVFQVDQENRARLLPDQTGCVTNGNPQCYEASGKVRTKGI
                     DFELQGMVTPLWEIGVGYTYADTRYIKDGDRSNEGKRFNTAVSPQNLFKISSMYTFDGK
                     LTNWRAGVNTYWQSKIYANITDTRDGNPVINKQKAYSTTDIIIGYTPMPQLDLQLNITN
                     VFDKVYYTSIGESIGYGTETYGEPRNVKISATYRF"
                     /name="TonB-dependent siderophore receptor"
     gene            2981..4942
                     /locus_tag="HBJ44_RS29015"
                     /old_locus_tag="HBJ44_029605"
     CDS             2981..4942
                     /locus_tag="HBJ44_RS29015"
                     /old_locus_tag="HBJ44_029605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_063938523.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNNRTAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIVWCAGGLV
                     AGILLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSVMGTNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEY
                     QPEAGRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            4942..5673
                     /gene="mobC"
                     /locus_tag="HBJ44_RS29020"
                     /old_locus_tag="HBJ44_029610"
     CDS             4942..5673
                     /gene="mobC"
                     /locus_tag="HBJ44_RS29020"
                     /old_locus_tag="HBJ44_029610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071653262.1"
                     /translation="MALERYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLAMEARGASGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            5680..6210
                     /locus_tag="HBJ44_RS29025"
                     /old_locus_tag="HBJ44_029615"
     CDS             5680..6210
                     /locus_tag="HBJ44_RS29025"
                     /old_locus_tag="HBJ44_029615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938511.1"
                     /translation="MALYMVFIEDAEDRDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERDKPAGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQISAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(6238..6417)
                     /locus_tag="HBJ44_RS29030"
                     /old_locus_tag="HBJ44_029620"
     CDS             complement(6238..6417)
                     /locus_tag="HBJ44_RS29030"
                     /old_locus_tag="HBJ44_029620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_021515244.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGKE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            6486..6863
                     /locus_tag="HBJ44_RS29035"
                     /old_locus_tag="HBJ44_029625"
     CDS             6486..6863
                     /locus_tag="HBJ44_RS29035"
                     /old_locus_tag="HBJ44_029625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938513.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQIETHERDGLRLSDETEKMASDALIAEIKALREEVAGLRQEMQAMRLLEH
                     KPETAETPAGTSRRWWQWGKR"
                     /name="hypothetical protein"
     gene            complement(6925..7224)
                     /locus_tag="HBJ44_RS29040"
                     /old_locus_tag="HBJ44_029630"
     CDS             complement(6925..7224)
                     /locus_tag="HBJ44_RS29040"
                     /old_locus_tag="HBJ44_029630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009365129.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_063938515.1"
                     /translation="MPYEVEWKQGAIEDVTALFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHTVR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(7214..7477)
                     /locus_tag="HBJ44_RS29045"
                     /old_locus_tag="HBJ44_029635"
     CDS             complement(7214..7477)
                     /locus_tag="HBJ44_RS29045"
                     /old_locus_tag="HBJ44_029635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938517.1"
                     /translation="MSNSTHYENANFLRELAERLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            7854..8939
                     /locus_tag="HBJ44_RS29050"
                     /old_locus_tag="HBJ44_029640"
     CDS             7854..8939
                     /locus_tag="HBJ44_RS29050"
                     /old_locus_tag="HBJ44_029640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006327147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="WP_063938519.1"
                     /translation="MAITIKSAVNVRSRVLIYSLLLFTGSAYAADIAESTFLTRDIGNG
                     LYEMSFDGKNNSLWAASSSSFDRDKGHGIVYQLEPSTLKTKSAVKIARLGFATALDEEH
                     ERLYIGNTLDGSVTVIDTLTGKELSVIPLTEGQDEAHFRPTREMVIDKKNQRLYVSSAA
                     DKGALWVIDMAVGKKIKTIDGLGHAVTGIALNKNGDNIYLVNGDGEIINLDASSYEIKK
                     RFVVEPEKKHFFLNVSLDEKNGRAFITDPDIPGVLVVDVNSGKILHRIDVINSLAILFN
                     PLRNEIYITHRNAKQVSIVDSNSYYVKTTIKTRLMPNSLSLSHDGENLYVSVKQEKKEM
                     STMKDYILKIELSKIAMEPEE"
                     /name="YncE family protein"
     Typing          complement(1601..2181)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.625"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4943..5670
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.885"
                     /coverage="100.0"
                     /evalue="3.84e-144"
ORIGIN
        1 tggtggtatg acttacctat ttctggtacc ggacaccacc ttaatcttcc tcgctcgacg
       61 tttacaggca atgactggga gtttgaaaga agtaaaagta aaactgcatt tgcaactctt
      121 gagcagaagt tcagtaatag ctggaagctg aaattttcaa cgctgcaaaa ctggagaaat
      181 gctgacctgt tagggacggc aacataccgg cataaagata cccgacaaag tatgtatcag
      241 caagtatggg gatcactgag ggaatataat tccaaaagct atgacctgtc gcttaatggg
      301 gactatgctt tatttggtcg tgttcatcaa ttcgtggctg gaacaactca gcaaaaatac
      361 accgataaag atacaggtta tacatattcc ccaatatata ttgagaatgt taatatattt
      421 gatcatggtt cacattcttc gaaaaaaccc atgaagatgt caggtgatgc atcctacgac
      481 gtcactgaac aaaaaagcgc ctacgcagca actcattttc tacttagtga taactggaaa
      541 atattgctgg gtggccgtct tgactggtat gaatatacaa gtaaaggtac atccattaat
      601 acaggttata aagttaccag aaatatcacc aaatatgcag gttcaacata caatcttgac
      661 gatcatcata ccatttatgg gagctatacc gatatattca atccacagtc aagcaccgac
      721 ctcaatggta aagtgatcaa gcctattgtt ggtaagaatt atgagacagg tattaagggt
      781 gagtatttta acggcttgct taatgcctct ctggcggtat ttcaggtaga ccaggaaaat
      841 cgagccaggc tgcttcccga tcagacaggg tgtgttacta atggcaatcc tcaatgctat
      901 gaggctagcg gtaaagtacg cacaaaaggg atagatttcg aacttcaggg catggttacg
      961 ccactctggg aaattggggt gggatataca tatgcagata cgcgatatat aaaagatgga
     1021 gatcgtagta atgaaggcaa gcgattcaat actgccgtgt cgccacaaaa tttatttaag
     1081 atttcgagca tgtacacctt tgacggaaaa ttaacaaact ggcgagcagg agtaaacacg
     1141 tactggcaaa gtaaaatata tgctaacata acagacacaa gggatggcaa tccggttatt
     1201 aataaacaaa aggcttattc aacaactgac attattattg gctatactcc aatgccacaa
     1261 ctggatttac agctcaatat aactaatgtc tttgataagg tttattatac atccataggt
     1321 gaatcgattg gatatggcac ggaaacatat ggagaaccaa gaaatgttaa aattagtgca
     1381 acctatagat tctaaccatg cggattgggt cttctcctcg gatttgcccc tataaatccg
     1441 tcttttgtgg ttccggcgta caaccccctg tttgccactt cagttgatgg ttaatcaaat
     1501 catgtagagg atcttcttga gatcctccat ggttcgtcgt aatcttttgc cctgtaaacg
     1561 aaaaaaccac ctggggaggt ggtttgatcg aaggttaagt cagttgggga actgcttaac
     1621 cgtggtaact ggctttcgca gagcacagca accaaatctg tccttccagt gtagccggac
     1681 tttggcgcac acttcaagag caaccgcgtg tttagctaaa caaatcctct gcgaactccc
     1741 agttaccaat ggctgctgcc agtggcgttt ttgcgtgtct ttccgggttg gactcaagtg
     1801 aacagttacc ggataaggcg cagcagtcgg gctgaacggg gagttcttgc ttacagccca
     1861 gcttggagcg aacgacctac accgagccga gataccagtg tgtgagctat gagaaagcgc
     1921 cacacttccc gtaagggaga aaggcggaac aggtatccgg taaacggcag ggtcggaaca
     1981 ggagagcgca agagggagcg acccgccgga aacggtgggg atctttaagt cctgtcgggt
     2041 ttcgcccgta ctgtcagatt catggttgag cctcacggct cccacagatg caccgaaaaa
     2101 gcgtctgttt atgtgaattc tggcaggagg gcgaagccta tggaaaaacg ccaccggcgc
     2161 ggccctgccg ttttgcctca catgttagtc ccctgcttat ccacggaatc tgtgggtaac
     2221 tttgcgagag ttagcagcgc ccgccgcagt ctcacggccg gagcgtagcg actgagtgag
     2281 ggaggaggcg caatatcccc cctcccgcag acggccaccg gacgcgcgga tgtgacatat
     2341 tgcaccgtgt taccgccttt cccttccctg ccgataaatc attcagatag gtgaagtctg
     2401 tcggttttct gctgaatatt ttacgttaac ctatctctgt tgcttttttc tccttgcgga
     2461 gaaatgcagg tcatccccgc tggggcgtgg ctgacttcag gaggaaatcc cctgcttacg
     2521 ctggctggac gccaccgacg cacggtcttt ccccctcttt ctggctcagc tcggctggac
     2581 gccggccacg ccagaaattc acccggcccc cgtcaggctc acgcctgacc acccacaaaa
     2641 acccacggtg ctgaacgcac ctgacgaagc agcaaagctg ctcctgattt tgcgggtggt
     2701 cagccgctcg ctatggcgaa ctctgacggg ggccggaaca ccggaaaaga ccgcactggg
     2761 gtgccgcctt ttcctctcac acgctggggc gtgctcgttg ttcctccggg gctgcgcctt
     2821 ttccgcttgg ggcggaaaga ggctggcctc cgtggtgaaa gctgaatgca atcaccacgg
     2881 acgtgaccgc cgccgccatg cctcggggca tgtccgcggc ttgtgcagca ggggctgcac
     2941 accacgccgg taaacccgtc gtctgaagga gagagattgc atgtttaata cggattcgct
     3001 tgcctggcag tggcatatcc cggagctggc ccggcagatt tacccgtggg tcagctggct
     3061 gtacagggag tattacggtc tggccggggc tttcctggtt atcggcctgt ttttcggtct
     3121 ggctgaactc tgggccggac ggatgcgttt tgtggggctg agtcataagg agcgacggga
     3181 gtatgccgat cgctataagc gcgataataa ccggactgcg ccattttcgg tgctggcttc
     3241 gctggattcg cccgggcgga tggtcagggc acggcggcat gtctggaact acgcgtactg
     3301 gctgaccggc gctgccggtc tggcccttct gggggggctg gttgtcgccg tgtttatcga
     3361 gagctgggga gaaaccgtcc tgcgtccgga cctctacgcg aacgcagatt attacctgct
     3421 gcagccggct aacctgttcg ggattgtctg gtgtgccgga gggcttgtcg ccggcatcct
     3481 tctggcgcgg ctgctggcat ttctgctgct tgacggtcat tttgcggccg ctgacgaggc
     3541 ggtgaatgcg aaactgaacc aggagagccg gcgcagcagc cagcggaccg gagaaatgac
     3601 cgacgtccgc cacctgcatt tcggggagcc ggtaccggtt aacgcgctgg ccgatttttc
     3661 cacagagcag gcccggaaac agcaggcggt gtttctgggt aaggatgagc agggccagcc
     3721 ggtgctggtc ccgcgtgata cctggcgcaa gaccaatatt cagatcctcg gcctgcccgg
     3781 gagcggcaag agcgtgatgg gcaccaacgc gctcattcgc tgcgtacgcg atttcgggga
     3841 cgcggtggtg tacttcgacc ccaaagggga cgcgtgggcg ccgcacgtct tccgggcgca
     3901 ctgcccggac ttcaccctgc tggatctgcg gccggggaaa ccggcgcagc tgaatctgtt
     3961 tcgcgatctc gaccagtacg ccctgaagaa tctgctggtt gccgggttta acctgagtga
     4021 gaccggcgac gtggcggacc actaccgcat cagcgagcag aaagcggcga agctgattgc
     4081 ggagcagttc ccgcaggggg caaacattca gcaggtgctg gcggcggcat acgcgctgcc
     4141 ggaggccctg aaaaaggacg tgaaggggct gatcaccaag ctggagaacg tggccgacct
     4201 gagcgtcctg cagacggaca gcggcatcga cgtggcgggg ataatcaacg gcggcggctg
     4261 cctgtacgtc atcggctcga tggacgatga ggcggtgatc cgggtgcaga agatgctgtt
     4321 tgcccgctgc gcgcaaatca tcattgcgcg ggatgagttc cggaggtggc cgcacgcgag
     4381 catcatgctc gatgagatta aatacctgct gtcgaagtac gtgctgaacg cgctggggac
     4441 gctgcgcagt cgggactgca acctgctgct cgcgcaccag tcgctgggcg acttcgggca
     4501 gtgcggccag gatctgcccg ctgagtttgt gaaaaccacg gtgctggata acacgccgat
     4561 ccgctggttc taccgggcgg ccagccagga gtccgcgcag tgggcggcgg gccagaccgg
     4621 ggaaatccgg gtggacgtgg agcgccgccg ggccagccgg gaggcgggga acgtggagca
     4681 tatcagcggg gacagcttca ttcagaagga ggcgcggccg ctgtttgacg tgaacaccct
     4741 gcagcacctg cctgacgggt ttgcggtgat gaccgggctg ggcgtggccc ggatggcgtt
     4801 cagcagcccg ctgcgggttg aacgccggga aatcccgctg cagcgcttcc cggtgctggc
     4861 gaaaacggac ccgctggcgg agtaccagcc ggaggccggg cggcagcggc cgggtgatga
     4921 tgattttgcg gggctgtact gatggcactg gagcgataca acgtcagcca cgcgaagcgg
     4981 caggcgcgga acgcggagaa aacccggctg accctgcgct ggctgcggga agagctgtgt
     5041 tccacggcgg agctggttgc gcgacggctg gggattgcgg cggtgcagcc ggtttaccgc
     5101 ttcctggaca gcctggtggc gaaagggctg ctggtccggg cgaaataccc ggttgacggc
     5161 cgtcaggtca gcgtgtgggg actgacgccg cacggggtgg cgttctcgtt cgacgaggac
     5221 gaaccgctga cggacgttat tccgtttcag ccgtcaaggg tgtcggcggc gcagctgccg
     5281 caccggctgg ccgtgcagtc cctgcgtctc gccatggagg cccggggggc ctccggctgg
     5341 cggtacctgc accgtatggc cctgaagggg atgaaggtgc cggacgcgct ggcggagctg
     5401 gacggcagaa cggtggcgtt tgaggtggag cgcaccgtga agagccgcag gcgctatcag
     5461 gaggtggtgt ccgggtatct gttcaatcgc aaagcgaacg gcatcgatga aatctggtac
     5521 atctgcccgg accgggcgac gcaggtcagg gtgcagcggg cgatcctgtc ggtggatgaa
     5581 atcgtgaatc cacagaccgg cgaggcccgc aaaacggcgg agctggaccg ggagcggctg
     5641 tttgcgtgct ttaaatttat gacaacggag tgagggatta tggcgctgta catggtattt
     5701 attgaagacg cggaggaccg ggacggcgag cgctgcatca tgccgaccag cgagcatctt
     5761 gaagatatgg tggtgctcgc ctggcagacc ggggcgtatg ttttctgttt tgtcgaccgg
     5821 gcccagcatc tggcgatgat gaaatccatc gggatgtatg acgagcggct caacagcgac
     5881 gaaaaagcca cgtacttttt caccggtaaa aatccgcaat gggaacggga caaacccgca
     5941 ggaatgaagc tgtgtgatta cctctatcgc aaggtggagc ggttcgccat cgagaacggt
     6001 gtgcaggtgg ttgatgccgg gaagtgctca ccggagcaga ttagcgcgat gattgaggtg
     6061 gtcagcgagc ggcacgggga aagcgcggtg aacgagaagc tgaaagcgga gctgatgcgg
     6121 gaatataaca gccgctattt taacgtgccg gtgcctgaac tgccggagga caaacccgcc
     6181 cgccggtggt ggccgttcgg caaaaaataa acggcagcac cggccatgcc ggtgctgtta
     6241 ttccttcccg agcttatccc gcatcacctg ccagagtgtt tccgcatcct cgtgcagccg
     6301 ctcgcacatg tcggccgggg tatccatatc cagctcgttc agtttctcga gtaacagcag
     6361 cgactggtcc ttaataaatt tagccatatt gagggcagtt ttttcctgct tagtcatgtt
     6421 ttctgtcctt ccgggtgtgg tgtgcttcct gtcgggtata ctgtctcaaa accggaggtg
     6481 agacaatggc gtgggtgacg gtgagacagg cgacggaact gacggggaaa gcccggagtt
     6541 ccctgtatag ggatatggcg aaaggccgcg tatcataccg tactgaagct gatggtggtc
     6601 gggtggtgga taccagcgaa cttatccggg tgtacggtga attaagacag attgagactc
     6661 acgagcggga cgggttgaga ctgtcggatg agactgaaaa aatggcctcc gacgcgttga
     6721 tcgcggagat aaaagcactc agggaggagg tggccggact gcggcaggaa atgcaggcca
     6781 tgagactgct ggaacacaaa ccagagacag cagagacacc ggccggaaca tcgcgccgat
     6841 ggtggcagtg gggaaaacgc tgacgcgctg tgcttgtcca accggcgacg gccacgcaat
     6901 gaattgcgcc cccatcaccg gttatcagcg aacggtatgg ggattctggc gctggccaac
     6961 cacggccaga acccggactt tcctggtctg gtcatcaaca tcatagagga catgctgtcc
     7021 catcagactg atccggcgaa cgtcctcgcc atagcgtgag tccacttcat ataggcgcgg
     7081 gaagtcggcc agtttgtccg ctgcagcgag aactcgatcg gtaacgttct gcgcatccca
     7141 cagggatgaa ttttccgcaa tgtagtcaaa caatgctgtc acatcctcga tggctccctg
     7201 cttccattcc acctcatacg gcatcgcgta gttcctgata gcggccatgc agtctctggc
     7261 ggagttgttc cgtggtcatg cccgggcgcg tgtcagcgcg ggcggcggtc actttagcgc
     7321 gaacctgatc ttcatattct gcctgggcca gttcttcgtt agccaggcgc tgcaacagtg
     7381 cagctttgtc cgggccacct tcaggcagta tgcggggcag tctctcggcc agttcccgca
     7441 ggaaattggc gttttcatag tgtgtactgt tgctcattgt ggagcctccc gggctgatac
     7501 gtcgatatga aggagtttag cagggggcgg tctgaaagaa tatgctcgtg tcagtcaggc
     7561 tgatgaagac gaaagctggt aaaaaagggc ttacgacacc cattttacgt caaactgcac
     7621 gacatgcagt tgcgtgcata gccatgcagc gggttaaaat ttatcaggcg cgtttccggc
     7681 ggttttccgg gtggtttgtt gccggtttta tcccgtagcc gccggaaatg ccctcagacc
     7741 gtctgagcgg tgcgcgtaat gcgacgttat ggtaaatcat gtgattaatg gtagtgataa
     7801 tcattatcac ttttgctata ttcttgtttc ttttattgta aggaaatgta agtatggcaa
     7861 tcacgattaa atccgccgtt aatgtccgta gcagagtatt aatatactct ttactactgt
     7921 ttactggttc agcgtatgcg gctgatatag ctgaatcaac attcctgact cgtgatatcg
     7981 gcaatggtct gtatgaaatg tcttttgatg gcaaaaataa tagtctctgg gcagcatcat
     8041 cgtcttcatt cgacagagat aagggccacg gaattgttta tcagttagaa ccatccactc
     8101 tgaaaacaaa atctgccgtt aaaatcgcac gtcttgggtt tgccacagca ctggatgagg
     8161 aacatgagcg actttatatc ggcaatactc tggacggctc agtgacggtc attgatactc
     8221 taaccggaaa agaactttca gtaattccgt taactgaggg acaggatgag gctcatttca
     8281 gaccgaccag agaaatggtc attgacaaaa aaaatcagcg tctttatgtc tccagcgccg
     8341 cagataaagg ggctctgtgg gttattgaca tggctgtcgg gaagaaaatt aaaaccattg
     8401 atggactggg gcacgctgtt actggaattg cgttaaataa aaatggggat aatatttatc
     8461 tggttaacgg ggatggtgag attattaacc tggatgccag tagctatgaa ataaaaaaac
     8521 ggtttgttgt agagcctgag aaaaaacact ttttccttaa tgtaagtctg gatgaaaaaa
     8581 acggacgtgc atttattacc gacccggata ttcccggggt gctggttgtt gatgtaaact
     8641 cgggaaaaat actacacaga attgatgtta taaattcact ggcgatttta tttaatcctt
     8701 tacggaacga aatctatatc acgcaccgta atgcaaaaca ggtcagcatt gtggacagta
     8761 attcttatta cgtaaaaaca acgataaaaa cccgcctgat gccaaacagt ctgtctttat
     8821 cacatgacgg tgaaaatctg tacgttagtg taaaacaaga aaaaaaagag atgagtacta
     8881 tgaaggatta catactcaaa atcgaattat ctaaaattgc tatggagcca gaagaatgaa
     8941 taaatattta gggctgagca tagtatatat gatgtataca tcatctgtta tggctgatga
     9001 ttctggcgca gttagtaaag ataaaaatat ttcaccagaa gaaacattaa ttgttcatgg
     9061 gaccgctgtt tctgaaagca caacagaagg aacagattca tacacaaccc agtcagccaa
     9121 tacagcatca aagatgaatc tgtcattccg ggatacacct cagtcaacta cagtcattac
     9181 gcgacgaaga atggatgatg tagcagcaaa aaatattatt gatgtggtta aaaccgctcc
     9241 tggattatat gtcagtacag cagaaggttc cggaagaccg aattttatgt ccagaggatt
     9301 taatgctaat gtcatgtatg aaggatttag cctgccgtgg aattcataca ttccaagttc
     9361 acaaactagt tcagctattt ttgaccgggt ggagatagtc cgcggggcaa caggtcttgt
     9421 tcagggtgcc ggagaccctt cagccgcgat aaatatgata cacaagcgtc cgacccggca
     9481 atatcaggcc agcactaaca tcggtgccgg acggtggaat gactaccggg gtggggttga
     9541 tgtatcagga ccactgaatg acagcgcgac cttacgcgga agggcagttg tggatggcga
     9601 aaacagtgag accttcaggg atagtgagaa acgcaggaat aacgtcattt acggaattat
     9661 ggaggcagat ctgactgata ataccctact tacgattggg ggatatcagc agtatgaata
     9721 taacaatcat
//
