LOCUS       NZ_CP053763             8401 bp    DNA     circular CON 17-APR-2024
DEFINITION  Shigella sonnei strain 506 plasmid pMHMC-012, complete sequence.
ACCESSION   NZ_CP053763
VERSION     NZ_CP053763.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07663113
            Assembly: GCF_013201485.1
KEYWORDS    RefSeq.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8401)
  AUTHORS   Hoffmann,M., Sanchez,M., Worley,J. and Bry,L.
  TITLE     clinical or host associated isolate for Shigella sonnei
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8401)
  AUTHORS   Hoffmann,M., Sanchez,M., Worley,J. and Bry,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) CFSAN, US FDA, 5001 Campus Drive, College
            Park, MD 20740, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAR-2020
            Assembly Method                   :: HGAP v. 3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 445.0x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013201485.1-RS_2024_04_17
            Annotation Date                   :: 04/17/2024 06:48:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,365
            Genes (coding)                    :: 4,629
            CDSs (with protein)               :: 4,629
            Genes (RNA)                       :: 132
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 100
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 736
            CDSs (without protein)            :: 736
            Pseudo Genes (ambiguous residues) :: 0 of 736
            Pseudo Genes (frameshifted)       :: 208 of 736
            Pseudo Genes (incomplete)         :: 523 of 736
            Pseudo Genes (internal stop)      :: 128 of 736
            Pseudo Genes (multiple problems)  :: 117 of 736
            Pseudo Genes (short protein)      :: 3 of 736
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP053763.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8401
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /strain="506"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /plasmid="pMHMC-012"
                     /geo_loc_name="USA:Boston"
                     /collection_date="2017"
                     /collected_by="Brigham and Women's Hospital"
     gene            complement(520..960)
                     /locus_tag="COO90_RS27340"
                     /old_locus_tag="COO90_27150"
     CDS             complement(520..960)
                     /locus_tag="COO90_RS27340"
                     /old_locus_tag="COO90_27150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005119689.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="hypothetical protein"
     gene            1178..1567
                     /locus_tag="COO90_RS27345"
                     /old_locus_tag="COO90_27155"
     CDS             1178..1567
                     /locus_tag="COO90_RS27345"
                     /old_locus_tag="COO90_27155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000102614.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGHD
                     NSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANNVR
                     VGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     gene            1611..>1799
                     /locus_tag="COO90_RS27350"
                     /old_locus_tag="COO90_27160"
                     /pseudo=""
     CDS             1611..>1799
                     /locus_tag="COO90_RS27350"
                     /old_locus_tag="COO90_27160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(1766..2965)
                     /gene="tet(A)"
                     /locus_tag="COO90_RS27355"
                     /old_locus_tag="COO90_27165"
     ARG             complement(1770..2965)
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            3071..3721
                     /gene="tetR(A)"
                     /locus_tag="COO90_RS27360"
                     /old_locus_tag="COO90_27170"
     CDS             3071..3721
                     /gene="tetR(A)"
                     /locus_tag="COO90_RS27360"
                     /old_locus_tag="COO90_27170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="WP_000164043.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            complement(3753..3995)
                     /locus_tag="COO90_RS27365"
                     /old_locus_tag="COO90_27175"
     CDS             complement(3753..3995)
                     /locus_tag="COO90_RS27365"
                     /old_locus_tag="COO90_27175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_000844627.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERGR
                     EAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="transposase"
     gene            complement(4301..5137)
                     /locus_tag="COO90_RS27370"
                     /old_locus_tag="COO90_27180"
     CDS             complement(4301..5137)
                     /locus_tag="COO90_RS27370"
                     /old_locus_tag="COO90_27180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            complement(5137..5940)
                     /gene="aph(3'')-Ib"
                     /locus_tag="COO90_RS27375"
                     /old_locus_tag="COO90_27185"
     ARG             complement(5141..5940)
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="99.63"
                     /coverage="100.0"
     gene            complement(6001..6816)
                     /gene="sul2"
                     /locus_tag="COO90_RS27380"
                     /old_locus_tag="COO90_27190"
     ARG             complement(6005..6816)
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(7276..7434)
                     /locus_tag="COO90_RS27580"
     CDS             complement(7276..7434)
                     /locus_tag="COO90_RS27580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032287700.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDRL
                     RGLEVEA"
                     /name="hypothetical protein"
     gene            8005..8154
                     /locus_tag="COO90_RS27385"
                     /old_locus_tag="COO90_27195"
     CDS             8005..8154
                     /locus_tag="COO90_RS27385"
                     /old_locus_tag="COO90_27195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     ARG             complement(4305..5137)
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          7797..7970
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          complement(7268..7873)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.593"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 aaaagcgttc ctcactgtat cgttcatgcc tgcatgaacg ggttattctt cctgactaca
       61 gaattacatc gctccttcag ttcatacatc acttttacat cacttataca tcacttttac
      121 atcacttata catcactttt acatcattcc ttcagctcat acgtcacttt catcctctgt
      181 gccatttgta catcaccccc ttcctgaacg tgctgacagc gggttttccg gctgcgccgg
      241 caacgcttca cggccacgca ataaattgcg tccccgttca ccgttttcgg atgggaagaa
      301 cgccggaaac aggacttacc ccggctgaat ggtgtgaatt tccgcgctat gcacttgtgc
      361 gcatactcat gcatgccgta aaaacagagc ctgcgcgttt ctggcgggtt ttcgggtggt
      421 ttgttgcctg ttttaccggt ttcccgtcag aaacgccctg agggcctctc aggcggtgct
      481 gataacagat gttatggtaa atcagagtca cctgcactgt tactctgaac cggagcgaaa
      541 cagccgcgac cagaatcccc gctttttttc ctgtgccagc gcgtcagcca gacggctaat
      601 ttcggcctgt agttgttccc tctcctcatt tcgcaggcgg ttttctgctt cccgcgccat
      661 cttgtcttca agcattaggg taacagtctg ctgcagagcg tccagtttct gctgaatgac
      721 gtctagctga gcattttccg cctggctaac gacagatttg gagtgtccct ccttgtgcct
      781 ttttggtgtc tcagaagtgc gcagctcgcc ataggcccga atcagttcac tggtctcgaa
      841 ctcccggcga tcattttttc ctgtgcgata gctgacaagg cctttagcca tgtcacgata
      901 taattgcgag cgcgactttc ctgttaattt cagtgtctct tgaactgtgt gccatgccat
      961 aaaagtgaca gtgtctcctg tgtgtaccgt attgtaacag cagaacatat tactgtttta
     1021 acacaaaagg tctgaggctg cccgtgaggc gtgaaaaata cacaaaaaac ctgttgacgg
     1081 tgtggttttg acgggcgtag tatttcgaat actgtatata aacacattgt tataaataca
     1141 gtttttgctg tttttaaaag tcaaagagga taattttatg agtacagagt taaatagcgg
     1201 tactgtcggt ggttttggtc acatgggacc aaatgacccc tacaatgatg gtcatagcgg
     1261 ctctggaagt tcgggaggta gttttaacgg tggtggtaat ggtggccatg ataatagtag
     1321 tggttcatct tatactgcat cctttggtct catcactggc cgacacccga taactgtccc
     1381 aggtacgaag aatattaatt ctgtcgatct ttctgctgtc attaataaca ttatacatca
     1441 tgaaggtgca cgcaataaca tgtacctaga tactgcgaac aatgtaagag tgggagtgac
     1501 gggcactggc tggcaatgtc tagcaacggc aggcatttcg gctgagggta aaagaacttt
     1561 ccgctaagcg atagactgta tgtaaacaca gtattgcaag gacgcggaac atgcctcatg
     1621 tggcggccag gacggccagc cgggatcggg atactggtcg ttaccagagc caccgacccg
     1681 agcaaaccct tctctatcag atcgttgacg agtattaccc ggcattcgct gcgcttatgg
     1741 cagagcaggg aaaggaattg ccgggctatg tgcaacggga atttgaagaa tttctccaaa
     1801 gcccgcgacg cagcgccggc aggcagagca agtagagggc agcgcctgca atccatgccc
     1861 acccgttcca cgttgttata gaagccgcat agatcgccgt gaagaggagg ggtccgacga
     1921 tcgaggtcag gctggtgagc gccgccagtg agccttgcag ctgcccctga cgttcctcat
     1981 ccacctgcct ggacaacatt gcttgcagcg ccggcattcc gatgccaccc gaagcaagca
     2041 ggaccatgat cgggaacgcc atccatcccc gtgtcgcgaa ggcaagcagg atgtagcctg
     2101 tgccgtcggc aatcattccg agcatgagtg cccgcctttc gccgagccgg gcggctacag
     2161 ggccggtgat cattgcctgg gcgagtgaat gcagaatgcc aaatgcggca agcgaaatgc
     2221 cgatcgtggt cgcgtcccag tgaaagcgat cctcgccgaa aatgacccaa agcgcggccg
     2281 gcacctgtcc gacaagttgc atgatgaaga agaccgccat cagggcggcg acgacggtca
     2341 tgccccgggc ccaccggaac gaagcgagcg ggttgagagc ctcccggcgt aacggccggc
     2401 gttcgccttt gtgcgactcc ggcaaaagga aacagcccgt caggaaattg aggccgttca
     2461 aggctgccgc ggcgaagaac ggagcgtggg gggagaaacc gcccatcagc ccaccgagca
     2521 caggtcccgc gaccatcccg aacccgaaac aggcgctcat gaagccgaag tgccgcgcgc
     2581 gctcatcgcc atcagtgata tcggcaatat aagcgccggc taccgcccca gtcgccccgg
     2641 tgatgccggc cacgatccgc ccgatataga gaacccaaag gaaaggcgcc gtcgccatga
     2701 tggcgtagtc gacagcagcg ccggccagcg agacgagcaa gaccggccgc cgcccgaaac
     2761 gatccgacag cgcgcccagc acaggtgcgc aggcaaattg catcaacgca tacagcgcca
     2821 gcagaatgcc atagtgggcg gtgacgtcgt tcgagtgaac cagatcgcgc aggaggcccg
     2881 gcagcaccgg cataatcagg ccgatgccga cagcgtcgag cgcgacagtg ctcagaatta
     2941 cgatcagggg tctgttgggt ttcacgtctg gcctccggac cagcctccgc tggtccgatt
     3001 gaacgcgcgg attctttatc actgataagt tggtggacat attatgttta tcagtgataa
     3061 agtgtcaagc atgacaaagt tgcagccgaa tacagtgatc cgtgccgccc tggacctgtt
     3121 gaacgaggtc ggcgtagacg gtctgacgac acgcaaactg gcggaacggt tgggggttca
     3181 gcagccggcg ctttactggc acttcaggaa caagcgggcg ctgctcgacg cactggccga
     3241 agccatgctg gcggagaatc atacgcattc ggtgccgaga gccgacgacg actggcgctc
     3301 atttctgatc gggaatgccc gcagcttcag gcaggcgctg ctcgcctacc gcgatggcgc
     3361 gcgcatccat gccggcacgc gaccgggcgc accgcagatg gaaacggccg acgcgcagct
     3421 tcgcttcctc tgcgaggcgg gtttttcggc cggggacgcc gtcaatgcgc tgatgacaat
     3481 cagctacttc actgttgggg ccgtgcttga ggagcaggcc ggcgacagcg atgccggcga
     3541 gcgcggcggc accgttgaac aggctccgct ctcgccgctg ttgcgggccg cgatagacgc
     3601 cttcgacgaa gccggtccgg acgcagcgtt cgagcaggga ctcgcggtga ttgtcgatgg
     3661 attggcgaaa aggaggctcg ttgtcaggaa cgttgaagga ccgagaaagg gtgacgattg
     3721 atcaggaccg ctgccggagc gcaacccact cactacagca gagccatgta gacaacatcc
     3781 cctccccctt tccaccgcgt cagacgcccg tagcagcccg ctacgggctt tttcatgccc
     3841 tgccctagcg tccaagcctc acggccgcgc tcggcctctc tggcggcctt ctggcgctcc
     3901 tgctgcggcg tccgctcgtg ggccgtggcg cgggtccgcg cgccggcctc gtgcgcctgg
     3961 cgctcgcggg cgaggtccag ggcggccgtc ttcacgttct gccttgcgca gatgagatag
     4021 atctagcgtg gactcaaggc tctcgcgaat ggctcgcgtt ggaaactttc attgacactt
     4081 gaggggcacc gcagggaaat tctcgtcctt gcgagaaccg gctatgtcgt gctgcgcatc
     4141 gagcctgcgc ccttggcttg tctcgcccct ctccgcgtcg ctacggggct tccaggggtc
     4201 gtttgcggga aggggcggaa tcctacgcta aggctttggc cagcgatatt ctccggtgag
     4261 attgatgtgt tcccagggga taggagaagt cgcttgatat ctagtatgac gtctgtcgca
     4321 cctgcttgat cgcggccgcg atagctagat cgcgttgctc ctcttctcca tccgcgttcc
     4381 aagctgcgga aaggcaccca taagcgtacg cctggtcgag caggcgacgc ggatcgacgt
     4441 ccagcgcacg agagaatgcg tccgccatct gtgcaatgcg tctaggatcg agacaaaggt
     4501 cgtctctgtc agccggatcg tagaacatat tggcggcgcc aaagcccact tcaccgacca
     4561 gaccgacggg atctatcacc agccagccgc gactggagaa catgatgttt tcatgatgca
     4621 gatcgccatg tagcccacgc agttccgagg cattgctcat catttgatcg gctataatcg
     4681 ccgcgtggac gtagtcagtt tgacaacctg cgttttgatc atcgcgcgcc cgctgaaaca
     4741 aagctgcaaa gcgatcccgg atcgggagaa gggcagaagg caggggttcc tcagatgcgg
     4801 catacagctt cgccattagt tccgctgcaa tttcggtcgc ctggtagtcg ccgtgctcgg
     4861 caacgatgtg agagagcatt cgctccccgg catattcgag caacatcaga ttgttctcac
     4921 gaccgagcaa ccggactgct cccctcccat tgcgccatac cagatagtcg gccccgcgca
     4981 gttcatcagc aatgtcttct ataggtttca atcccttgac gattgcagga gtcccgtctg
     5041 gcaatgaaac tttccaaacg aggctggaaa aggtgtccgc aatgagaaca ggttgcgaaa
     5101 cgtgccaatg agcaggaaaa acaggcggca tgaacatcaa ccccaagtca gagggtccaa
     5161 tcgcagatag aaggcaaggc gttcgcggtc gggggcttcg atccccaata cattgaatag
     5221 gacagcgaag gcgcgctctg cttcatctgg cgctgcccag ttctcttcgg cgttagcaat
     5281 catgagtgcc aaatcggcat agcgatctgc tgttccgagc cgcccaaggt cgatcagacc
     5341 cgtgcattga agagttttag ggtccaccat gaagttcggc atgcagggat caccatggca
     5401 aacaaccata tcggtgcgct cttggtcgag ccgcaccggt agctctcgtt cgacacgagc
     5461 caaaagatcg agctgcggcg tactcttgtc ctcgtccggt aagaagtcgg gattgacggc
     5521 attgcgggac accacatcaa cggcgcgtcc gaacattcgc gacagcctgc gctcaaactg
     5581 acattgatca accgataggc tgtgaacagc gccaagttgc tgccccattg acggccacgc
     5641 tttgagcaaa tccgctccag acagatcagc cgccggtact cccggaattg ccgttatcac
     5701 caagcatgca ccctcctgtt cctcctgcca gttgatgacc tcggggcaag ccacacctcg
     5761 acctttgagc caaatgaggc ggtcacgctc tccagcgagc tcaccgcggc gggaagcagg
     5821 tgcgattttc gcgaaggcat gcccgtcacc acgtcgaaaa acaaaatcac cagattctcc
     5881 gcctctgaca ggcaaccagt cagaatgcga ttcaccaaaa aaaatattag ttcgattcaa
     5941 tggaggttcc ttcagttttc tgatgaagcg cgaatataga gaaatatccc gaatgtgcag
     6001 ttaacgaatt cttgcggttt ctttcagcgc cgccaatacc gccagcccgt cgcgcaaggg
     6061 gcgcggctcg tgtgtgcgga tgaagtcagc tccacctgcg gcggcggcaa gctctgcagc
     6121 gagtgtcgcg gccccgacat cccccggacc acggcctgtg agcgcgcgca gaaaggattt
     6181 gcgcgaaaca gacagaagca ccggcaaatc gaagcgcagc cgcaattcat cgaaccgcgc
     6241 cagcaccgag agcgaggttt cgggagcagc ccccagaaaa aaccccatgc cgggatcaag
     6301 gacaaggcgg ttgcgtttga taccggcacc cgtcagcgcc gcgatgcgcg cgtcaaagaa
     6361 cgccgcaatg tgatccatga tgtcgccagc gggtgcctcg cgccgatctg cctgcccgtc
     6421 ttgcaccgaa tgcataacga cgagtttggc agatgatttc gccaattgcg gatagaacgc
     6481 agcgtctgga aaaccgcgaa tatcattgag ataggccaca ccacgcgaca aggcataggc
     6541 ttgcgtcgcg ggttgataac tgtcgagcga gacgggaatg ccatctgcct tgagcgcgtc
     6601 cagcaccggc gcgatacgcg cgatttctgt gtcggacgaa acaggcgcgg cgtcgggatt
     6661 gctggatgcc ggaccgaggt cgatcacatc tgccccctcg gccatcagct tacgcgcctg
     6721 cgcaatggct gcgtctggcg ccagataccg gcctccatcg gagaaactgt ccgaggttat
     6781 gttgacgatg ccgaaaatga tgagcgattt attcatgggg gcttctataa taataataat
     6841 cgagcatgag tctcatacgg atgctcgggt cgaaagggaa tccccaggcg agtaacctgt
     6901 ttgcggtgat ccgtttcatg atgatgtatc tgtagcattt tggaaataaa tatatcatct
     6961 ctcaataagt agatatattt atcttttttg cttctttggt gctgcaagtc accaatacga
     7021 gggaaacctg ttacggagcg gtttaaccgt aatcaacagt gactggtgtc atgttcggaa
     7081 gattaattga tagtaatcta caaaggatct tcttgagatc cttttcaatg aatcgtaatc
     7141 tcttgctctg taaacgaaaa aaccgcctgg ggaggcggtt tgttcgaagg ttaagtcagt
     7201 tggggaactg cttaacccgg taacagggtt cacgagaccg ccattaccaa aactgttctt
     7261 tcagtgtagc cgcagtcagg cctctacttc aagacctctt aatctatctt ctcgtgcact
     7321 cctgttacta atggctgctg ccagtggcgt tttgtcgtgt ctttccgggt tggactcaag
     7381 ttgatagtta ccggacaagg cgcagcggtc ggactgaacg gggggttcgt gcatacagtc
     7441 cagcttggag cgaactgcct tcacggaact gagtgtcagg cgtggaatga gaaaccgcgg
     7501 ccataacagc ggaatgacac cggtaaaccg aaaggcagga acaggagagc gcacgaggga
     7561 gctaccaggg ggaaacgcct gggatcttta tagtcctgtc gggtttcgcc accactgatt
     7621 tgagcgtcag attttgcgat gcttgtcagg ggggcggagc ctatggaaaa acggcttcgc
     7681 cgtggcctta tctcttcttt gctaagtgtc ttcctggtat cttccaggaa atttccgtcc
     7741 ccgtccgtaa gccggtaccg ctcgccgcag ccgaacgacc gagcgtagcg agtcagtgag
     7801 cgaggaagcg gaatatctcc tgtatcgcat attctgctga cgcgccggtg ctgccttttt
     7861 tctcctgcca catgaagcac ttcactgatt ttcgcaatcg tggcaacata gtaagccagt
     7921 atacactccg ctagcgctac atgactggtt cagggctgcg ccccgaaacc cgctaacaac
     7981 ctctcagcta ctggatatca ccccatgcca tctgaatatg atgaacaact ggattcgact
     8041 gatacgcagc ttcccggcga atatgacctt tttgatttaa tggcgcaggt tactgaagag
     8101 aacctgcatg cccctgtgga ttttgggtcg cctgtcggcc gggaagcgtt atagcttttt
     8161 cgcttgcccg cgtacgtggt aatactccgg tattaccttt tatttcttta ttttcatgta
     8221 attacagtgg atttttaata cccgggtatt actcgtgaaa acgaaatatt cctttttttt
     8281 cttttaattc atgcagttgc gatgaaaatc gcggctgcgt taggtgtatg accattttga
     8341 ggttgaaaaa taatcaacca tggcgggtga tttacgcagg ttaaggggag atcgtgacaa
     8401 a
//
