LOCUS       NZ_CP053741             8176 bp    DNA     circular CON 13-JUN-2023
DEFINITION  Escherichia coli strain CP8-3_Sichuan plasmid pCP8-3-IncQ, complete
            sequence.
ACCESSION   NZ_CP053741
VERSION     NZ_CP053741.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14944002
            Assembly: GCF_013201235.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8176)
  AUTHORS   Li,R., Du,P., Zhang,P., Li,Y., Yang,X., Wang,Z., Wang,J. and Bai,L.
  TITLE     Comprehensive genomic understanding of coevolution of mcr genes in
            E. coli strains via nanopore sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8176)
  AUTHORS   Li,R., Li,Y., Zhang,P. and Bai,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) College of Veterinary Medicine (Institute of
            Comparative Medicine), Yangzhou University, Wenhui Road, Yangzhou,
            Jiangsu 225009, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2019
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/13/2023 03:37:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,918
            CDSs (total)                      :: 4,800
            Genes (coding)                    :: 4,586
            CDSs (with protein)               :: 4,586
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 214
            CDSs (without protein)            :: 214
            Pseudo Genes (ambiguous residues) :: 0 of 214
            Pseudo Genes (frameshifted)       :: 68 of 214
            Pseudo Genes (incomplete)         :: 150 of 214
            Pseudo Genes (internal stop)      :: 33 of 214
            Pseudo Genes (multiple problems)  :: 37 of 214
            Pseudo Genes (short protein)      :: 4 of 214
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP053741.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8176
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="CP8-3_Sichuan"
                     /isolation_source="slaughterhouse"
                     /host="pig"
                     /db_xref="taxon:562"
                     /plasmid="pCP8-3-IncQ"
                     /geo_loc_name="China: Sichuan"
                     /collection_date="2016-07"
     gene            1..840
                     /locus_tag="HP430_RS24185"
                     /old_locus_tag="HP430_24240"
     CDS             1..840
                     /locus_tag="HP430_RS24185"
                     /old_locus_tag="HP430_24240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="WP_000190708.1"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
                     /name="helicase RepA family protein"
     gene            827..1678
                     /gene="repC"
                     /locus_tag="HP430_RS24190"
                     /old_locus_tag="HP430_24245"
     CDS             827..1678
                     /gene="repC"
                     /locus_tag="HP430_RS24190"
                     /old_locus_tag="HP430_24245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C, IncQ-type"
                     /protein_id="WP_000240536.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
                     /name="repC"
     gene            1973..>2389
                     /locus_tag="HP430_RS24195"
                     /old_locus_tag="HP430_24255"
                     /pseudo=""
     CDS             1973..>2389
                     /locus_tag="HP430_RS24195"
                     /old_locus_tag="HP430_24255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /name="dihydropteroate synthase"
     gene            2747..3769
                     /locus_tag="HP430_RS24200"
                     /old_locus_tag="HP430_24265"
     CDS             2747..3769
                     /locus_tag="HP430_RS24200"
                     /old_locus_tag="HP430_24265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821153.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA]; GO:0008324 - monoatomic cation
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="WP_000083351.1"
                     /translation="MSQSHAHDHGHDHDHDHTPTVTSANERKVLVSFFLIFGFMLVEAV
                     GGVLSGSLALLADAGHMLTDAVALALAYAAFRFGRRAADSKRTFGYLRFEVIAGFLNAV
                     TLFAIVAWIAYEAWERLQAPPVILAGPMMIVAVLGLLINVLVLWIMTRGETDHVNVKGA
                     ILHVMGDLLGSVGAIVAAIVIYFTGWTPIDPILSVLVAALILRSAWKLLAKSIHILLEG
                     APEDASPEKVEQGLMSTVPGLAAVSHVHVWQLTSGRTMATLHVRPNVDEEARAVVKRVE
                     VVLREQFSIEHATVGIDWNSEADENICSLQPTTDRQDHSGHGHSEHNHDHDHSAPGHKH
                     "
                     /name="cation diffusion facilitator family transporter"
     gene            3813..4190
                     /locus_tag="HP430_RS24205"
                     /old_locus_tag="HP430_24270"
     CDS             3813..4190
                     /locus_tag="HP430_RS24205"
                     /old_locus_tag="HP430_24270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821152.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WP_000203935.1"
                     /translation="MTSRKTTSSAIAHCSTSSHGATTDVLALSQSDVAVLAETFRLLGD
                     QSRLKILLQCMRGSVAVGDIADSLDLSQSLVSHHLRLLRGARLVRGERQAKHIFYGIAD
                     QHVSQVLQDMAVHISEDRNDD"
                     /name="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            complement(<4295..4456)
                     /locus_tag="HP430_RS24210"
                     /old_locus_tag="HP430_24275"
                     /pseudo=""
     CDS             complement(<4295..4456)
                     /locus_tag="HP430_RS24210"
                     /old_locus_tag="HP430_24275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(5054..5338)
                     /locus_tag="HP430_RS24215"
                     /old_locus_tag="HP430_24280"
     CDS             complement(5054..5338)
                     /locus_tag="HP430_RS24215"
                     /old_locus_tag="HP430_24280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein C"
                     /protein_id="WP_000238497.1"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
                     /name="mobilization protein C"
     gene            5642..>6175
                     /locus_tag="HP430_RS24670"
                     /pseudo=""
     CDS             5642..>6175
                     /locus_tag="HP430_RS24670"
                     /inference="COORDINATES: protein motif:HMM:NF015354.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /name="MobA/MobL family protein"
     gene            6285..6698
                     /locus_tag="HP430_RS24675"
     CDS             6285..6698
                     /locus_tag="HP430_RS24675"
                     /inference="COORDINATES: protein motif:HMM:NF036929.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncQ-type mobilization protein MobB"
                     /protein_id="WP_010890157.1"
                     /translation="MNAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTQQAS
                     EAQAAEWLKAQRQTGAAWVELAKELREVAAEVSSAAQSARSASRGWHWKLWLTVMLASM
                     MPTVVLLIASLLLLDLTPLTTEDGSIWLRLVAR"
                     /name="IncQ-type mobilization protein MobB"
     gene            6743..7666
                     /locus_tag="HP430_RS24680"
     CDS             6743..7666
                     /locus_tag="HP430_RS24680"
                     /inference="COORDINATES: protein motif:HMM:NF028103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-primase RepB domain-containing protein"
                     /protein_id="WP_014344452.1"
                     /translation="MGCERFDIGVRDATTGQMMNREWSAAEVLQNTPWLKRMNAQGNDV
                     YIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKNYQAWVKVADAAGGELR
                     GQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQPWVLLRESKGKTATAG
                     PALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALDEYRSEMAGLVKRFGDD
                     LSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEADYIERTVSKVMGLPSVQ
                     LARAELARAPAPRQRGMDRGGPDFSM"
                     /name="DNA-primase RepB domain-containing protein"
     gene            7727..7939
                     /locus_tag="HP430_RS24225"
                     /old_locus_tag="HP430_24290"
     CDS             7727..7939
                     /locus_tag="HP430_RS24225"
                     /old_locus_tag="HP430_24290"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000455501.1"
                     /translation="MEYEKSASGSVYLIKSDKGYWLPGGFGYTSNKAEAGRFSVADMAS
                     LNLDGCTLSLFREDKPFGPGKFLGD"
                     /name="hypothetical protein"
     gene            7941..8147
                     /gene="urfF"
                     /locus_tag="HP430_RS24230"
                     /old_locus_tag="HP430_24295"
     CDS             7941..8147
                     /gene="urfF"
                     /locus_tag="HP430_RS24230"
                     /old_locus_tag="HP430_24295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid repression protein F"
                     /protein_id="WP_112923071.1"
                     /translation="MKHQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDEY
                     EALRECLEELRAAQGGGSDPASA"
                     /name="urfF"
     ARG             1974..2488
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide resistant sul"
                     /tool="rgi"
                     /drug_class="sulfonamide antibiotic"
                     /identity="100.0"
                     /coverage="63.1"
     Typing          5293..5448
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.28e-76"
     Typing          19..840
                     /name="IncQ1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          5538..7663
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggctaccc ataagcctat caatattctg gaggcgttcg cagcagcgcc gccaccgctg
       61 gactacgttt tgcccaacat ggtggccggt acggtcgggg cgctggtgtc gcccggtggt
      121 gccggtaaat ccatgctggc cctgcaactg gccgcacaga ttgcaggcgg gccggatctg
      181 ctggaggtgg gcgaactgcc caccggcccg gtgatctacc tgcccgccga agacccgccc
      241 accgccattc atcaccgcct gcacgccctt ggggcgcacc tcagcgccga ggaacggcaa
      301 gccgtggctg acggcctgct gatccagccg ctgatcggca gcctgcccaa catcatggcc
      361 ccggagtggt tcgacggcct caagcgcgcc gccgagggcc gccgcctgat ggtgctggac
      421 acgctgcgcc ggttccacat cgaggaagaa aacgccagcg gccccatggc ccaggtcatc
      481 ggtcgcatgg aggccatcgc cgccgatacc gggtgctcta tcgtgttcct gcaccatgcc
      541 agcaagggcg cggccatgat gggcgcaggc gaccagcagc aggccagccg gggcagctcg
      601 gtactggtcg ataacatccg ctggcagtcc tacctgtcga gcatgaccag cgccgaggcc
      661 gaggaatggg gtgtggacga cgaccagcgc cggttcttcg tccgcttcgg tgtgagcaag
      721 gccaactatg gcgcaccgtt cgctgatcgg tggttcaggc ggcatgacgg cggggtgctc
      781 aagcccgccg tgctggagag gcagcgcaag agcaaggggg tgccccgtgg tgaagcctaa
      841 gaacaagcac agcctcagcc acgtccggca cgacccggcg cactgtctgg cccccggcct
      901 gttccgtgcc ctcaagcggg gcgagcgcaa gcgcagcaag ctggacgtga cgtatgacta
      961 cggcgacggc aagcggatcg agttcagcgg cccggagccg ctgggcgctg atgatctgcg
     1021 catcctgcaa gggctggtgg ccatggctgg gcctaatggc ctagtgcttg gcccggaacc
     1081 caagaccgaa ggcggacggc agctccggct gttcctggaa cccaagtggg aggccgtcac
     1141 cgctgatgcc atggtggtca aaggtagcta tcgggcgctg gcaaaggaaa tcggggcaga
     1201 ggtcgatagt ggtggggcgc tcaagcacat acaggactgc atcgagcgcc tttggaaggt
     1261 atccatcatc gcccagaatg gccgcaagcg gcaggggttt cggctgctgt cggagtacgc
     1321 cagcgacgag gcggacgggc gcctgtacgt ggccctgaac cccttgatcg cgcaggccgt
     1381 catgggtggc ggccagcatg tgcgcatcag catggacgag gtgcgggcgc tggacagcga
     1441 aaccgcccgc ctgctgcacc agcggctgtg tggctggatc gaccccggca aaaccggcaa
     1501 ggcttccata gataccttgt gcggctatgt ctggccgtca gaggccagtg gttcgaccat
     1561 gcgcaagcgc cgccagcggg tgcgcgaggc gttgccggag ctggtcgcgc tgggctggac
     1621 ggtaaccgag ttcgcggcgg gcaagtacga catcacccgg cccaaggcgg caggctgacc
     1681 ccccccactc tattgtaaac aagacatttt tatcttttat attcaatggc ttattttcct
     1741 gctaattggt aataccatga aaaataccat gctcagaaaa ggcttaacaa tattttgaaa
     1801 aattgcctac tgagcgctgc cgcacagctc cataggccgc tttcctggct tatgctattc
     1861 tgctcctgca gctaatggat caccgcaaac aggttactcg cctggggatt ccctttcgac
     1921 ccgagcatcc gtatgagact catgctcgat tattattatt atagaagccc ccatgaataa
     1981 atcgctcatc attttcggca tcgtcaacat aacctcggac agtttctccg atggaggccg
     2041 gtatctggcg ccagacgcag ccattgcgca ggcgcgtaag ctgatggccg agggggcaga
     2101 tgtgatcgac ctcggtccgg catccagcaa tcccgacgcc gcgcctgttt cgtccgacac
     2161 agaaatcgcg cgtatcgcgc cggtgctgga cgcgctcaag gcagatggca ttcccgtctc
     2221 gctcgacagt tatcaacccg cgacgcaagc ctatgccttg tcgcgtggtg tggcctatct
     2281 caatgatatt cgcggttttc cagacgctgc gttctatccg caattggcga aatcatctgc
     2341 caaactcgtc gttatgcatt cggtgcaaga cgggcaggca gatcggcgcg gtcatgggct
     2401 taccatcgac cttcattgtc atgtctctga tcggcttggc cggcgtggtg ctggcgctct
     2461 ggctgtggcc ggccagcgac ccttctgacg tggtgcatga ccaccccagc ttgccgccgg
     2521 atcaccctca tttgcgcaca cacgcagacc gaggcaagca ccatcaccag ctgattgtgg
     2581 atgacctgca ccgtatctgg ccgaaaggat aggcacattt tctcgttgcg gcaccacaac
     2641 gtatggcttc ggcggctggg aagggtgagc gaaaatactt cacatgaaca tgtattcatg
     2701 tgttatattt tcaccatagt caaacactca accagcagag gcgcccatgt cccaatccca
     2761 tgcacatgac catggtcacg accatgatca cgaccacacc cccacagtga ccagcgccaa
     2821 cgagcgcaag gttctggttt ccttcttcct gatcttcggc ttcatgctcg tggaagctgt
     2881 cggcggcgtg ctgtcgggct cgctagccct acttgccgat gctggccaca tgctgaccga
     2941 cgccgtggca cttgccctgg cttacgctgc cttccgattc ggtcgccgcg cagccgacag
     3001 caaacgcaca ttcggttacc tgcgcttcga ggtcatcgcc ggcttcctga acgccgtgac
     3061 cctgttcgcg atcgtcgcat ggatcgccta cgaggcgtgg gagcggctgc aggcgccgcc
     3121 cgtcatcctc gccggcccga tgatgatcgt cgccgtgctc ggcttactga tcaacgtcct
     3181 cgtgctgtgg atcatgaccc gcggcgaaac cgatcacgtc aatgtgaaag gtgccattct
     3241 ccacgtgatg ggtgacctgc tgggctctgt cggcgccatc gttgcagcga tcgtcatcta
     3301 tttcaccggc tggacgccga tcgaccccat tctgtcggtc ttggtggcag cgttgattct
     3361 gcgcagcgcc tggaagctcc tggccaagtc gatccatatc ctgctggaag gcgcgccaga
     3421 ggatgcttcg ccggaaaagg tggaacaagg cctgatgagc actgtacccg gcctggcagc
     3481 tgtcagccac gttcatgtgt ggcaactcac ttccggtcgc acgatggcca cactgcacgt
     3541 tcgacccaat gtggacgagg aggcgcgagc cgtggtcaag cgcgtcgagg tggtgcttcg
     3601 agagcaattc agcatcgaac acgccacagt cggaatcgac tggaactcgg aggctgacga
     3661 gaacatctgc agcctgcaac ccacgacaga ccgtcaggac cacagcggcc atggccacag
     3721 tgagcacaat catgaccatg accactccgc tcctggtcac aagcattgag gccgtgcgag
     3781 ccggctcgca gttcacttga gggtttgaat ccatgacatc acgcaaaacc accagctccg
     3841 ctattgctca ctgttcgacc tccagccacg gcgcgaccac tgatgtcctc gcactgtcgc
     3901 aaagtgacgt cgctgttctg gccgagacct tccgcctgct gggcgaccaa tcgcgattga
     3961 aaatcctgtt gcagtgcatg cgcggatccg tcgcagtggg cgacattgcc gattcgctag
     4021 acctgtcgca atccttggtc agccatcacc tgcgcctgtt gcgcggcgcg cgcctcgtgc
     4081 gcggcgagcg ccaggccaag cacatcttct acggcatcgc cgaccagcac gtcagccagg
     4141 tactgcagga catggcggtt cacatttcgg aggacagaaa cgacgattga ctgaacgtgt
     4201 gagtgcgcgc cggcctactc cttcaagggg taggcttttt tttggctatc aatctagaga
     4261 aacaccgacg cgccttgctg aatgcctaga accccacata gcccggcaat tcctttccct
     4321 gctctgccat aagcgcagcg aatgccgggt aatactcgtc aacgatctga tagagaaggg
     4381 tttgctcggg tcggtggctc tggtaacgac cagtatcccg atcccggctg gccgtcctgg
     4441 ccgccacatg aggcatgttc cgcgtccttg caatactgtg tttacataca gtctatcgct
     4501 tagcggaaag ttcttttacc ctcagccgaa atgcctgccg ttgctagaca ttgccagcca
     4561 gtgcccgtca ctcccgtact aactgtcacg aacccctgca ataactgtca cgcccccctg
     4621 caataactgt cacgaacccc tgcaataact gtcacgcccc cctgcaataa ctgtcacgaa
     4681 cccctgcaat aactgtcacg cccccaaacc tgcaaaccca gcaggggcgg gggctggcgg
     4741 ggtgttggaa aaatccatcc atgattatct aagaataatc cactaggcgc ggttatcagc
     4801 gcccttgtgg ggcgctgctg cccttgccca atatgcccgg ccagaggccg gatagctggt
     4861 ctattcgctg cgctaggcta cacaccgccc caccgctgcg cggcaggggg aaaggcgggc
     4921 aaagcccgct aaaccccaca ccaaaccccg cagaaatacg ctggagcgct tttagccgct
     4981 ttagcggcct ttccccctac ccgaagggtg ggggcgcgtg tgcagccccg cagggcctgt
     5041 ctcggtcgat cattcagccc ggctcatcct tctggcgtgg cggcagaccg aacaaggcgc
     5101 ggtcgtggtc gcgttcaagg tacgcatcca ttgccgccat gagccgatcc tccggccact
     5161 cgctgctgtt caccttggcc aaaatcatgg cccccaccag caccttgcgc cttgtttcgt
     5221 tcttgcgctc ttgctgctgt tcccttgccc gcacccgctg aatttcggca ttgattcgcg
     5281 ctcgttgttc ttcgagcttg gccagccgat ccgccgcctt gttgctcccc ttaaccatct
     5341 tgacacccca ttgttaatgt gctgtctcgt aggctatcat ggaggcacag cggcggcaat
     5401 cccgacccta ctttgtaggg gagggcgcac ttaccggttt ctcttcgaga aactggccta
     5461 acggccaccc ttcgggcggt gcgctctccg agggccattg catggagccg aaaagcaaaa
     5521 gcaacagcga ggcagcatgg cgatttatca ccttacggcg aaaaccggca gcaggtcggg
     5581 cggccaatcg gccagggcca aggccgacta catccagcgc gaaggcaagt atgcccgcga
     5641 catggatgaa gtcttgcacg ccgaatccgg gcacatgccg gagttcgtcg agcggcccgc
     5701 cgactactgg gatgctgccg acctgtatga acgcgccaat gggcggctgt tcaaggaggt
     5761 cgaatttgcc ctgccggtcg agctgaccct cgaccagcag aaggcgctgg cgtccgagtt
     5821 cgcccagcac ctgaccggtg ccgagcgcct gccgtatacg ctggccatcc atgccggtgg
     5881 cggcgagaac ccgcactgcc acctgatgat ctccgagcgg atcaatgacg gcatcgagcg
     5941 gcccgccgct cagtggttca agcggtacaa cggcaagacc ccggagaagg gcggggcaca
     6001 gaagaccgaa gcgctcaagc ccaaggcatg gcttgagcag acccgcgagg catgggccga
     6061 ccatgccaac cgggcattag agcgggctgg ccacgacgcc cgcattgacc acagaacact
     6121 tgaggcgcag ggcatcgagc gcctgcccgg tgttcacctg gggccgaacg tggtggagat
     6181 ggaaggccgg ggcatccgca ccgaccgggc agacgtggcc ctgaacatcg acaccgccaa
     6241 cgcccagatc atcgacttac aggaataccg ggaggcaata gaccatgaac gcaatcgaca
     6301 gagtgaagaa atccagaggc atcaacgagt tagcggagca gatcgaaccg ctggcccaga
     6361 gcatggcgac actggccgac gaagcccggc aggtcatgag ccagacccag caggccagcg
     6421 aggcgcaggc ggcggagtgg ctgaaagccc agcgccagac aggggcggca tgggtggagc
     6481 tggccaaaga gttgcgggag gtagccgccg aggtgagcag cgccgcgcag agcgcccgga
     6541 gcgcgtcgcg ggggtggcac tggaagctat ggctaaccgt gatgctggct tccatgatgc
     6601 ctacggtggt gctgctgatc gcatcgttgc tcttgctcga cctgacgcca ctgacaaccg
     6661 aggacggctc gatctggctg cgcttggtgg cccgatgaag aacgacagga ctttgcaggc
     6721 cataggccga cagctcaagg ccatgggctg tgagcgcttc gatatcggcg tcagggacgc
     6781 caccaccggc cagatgatga accgggaatg gtcagccgcc gaagtgctcc agaacacgcc
     6841 atggctcaag cggatgaatg cccagggcaa tgacgtgtat atcaggcccg ccgagcagga
     6901 gcggcatggt ctggtgctgg tggacgacct cagcgagttt gacctggatg acatgaaagc
     6961 cgagggccgg gagcctgccc tggtagtgga aaccagcccg aagaactatc aggcatgggt
     7021 caaggtggcc gacgccgcag gcggtgaact tcgggggcag attgcccgga cgctggccag
     7081 cgagtacgac gccgacccgg ccagcgccga cagccgccac tatggccgct tggcgggctt
     7141 caccaaccgc aaggacaagc acaccacccg cgccggttat cagccgtggg tgctgctgcg
     7201 tgaatccaag ggcaagaccg ccaccgctgg cccggcgctg gtgcagcagg ctggccagca
     7261 gatcgagcag gcccagcggc agcaggagaa ggcccgcagg ctggccagcc tcgaactgcc
     7321 cgagcggcag cttagccgcc accggcgcac ggcgctggac gagtaccgca gcgagatggc
     7381 cgggctggtc aagcgcttcg gtgatgacct cagcaagtgc gactttatcg ccgcgcagaa
     7441 gctggccagc cggggccgca gtgccgagga aatcggcaag gccatggccg aggccagccc
     7501 agcgctggca gagcgcaagc ccggccacga agcggattac atcgagcgca ccgtcagcaa
     7561 ggtcatgggt ctgcccagcg tccagcttgc gcgggccgag ctggcacggg caccggcacc
     7621 ccgccagcga ggcatggaca ggggcgggcc agatttcagc atgtagtgct tgcgttggta
     7681 ctcacgcctg ttatactatg agtactcacg cacagaaggg ggttttatgg aatacgaaaa
     7741 aagcgcttca gggtcggtct acctgatcaa aagtgacaag ggctattggt tgcccggtgg
     7801 ctttggttat acgtcaaaca aggccgaggc tggccgcttt tcagtcgctg atatggccag
     7861 ccttaacctt gacggctgca ccttgtcctt gttccgcgaa gacaagcctt tcggccccgg
     7921 caagtttctc ggtgactgat atgaaacacc aaaaggacaa gcagaccggc gacctgctgg
     7981 ccagccctga cgctgtacgc caagcgcgat atgccgagcg catgaaggcc aaagggatgc
     8041 gtcagcgcaa gttctggctg accgacgacg aatacgaggc gctgcgcgag tgcctggaag
     8101 aactcagagc ggcgcagggc gggggtagtg accccgccag cgcctaacca ccaactgcct
     8161 gcaaaggagg caatca
//
