LOCUS       NZ_CP053727             8197 bp    DNA     circular CON 13-JUN-2023
DEFINITION  Escherichia coli strain CP66-6_Sichuan plasmid pCP66-6-IncQ,
            complete sequence.
ACCESSION   NZ_CP053727
VERSION     NZ_CP053727.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14944005
            Assembly: GCF_013201195.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8197)
  AUTHORS   Li,R., Du,P., Zhang,P., Li,Y., Yang,X., Wang,Z., Wang,J. and Bai,L.
  TITLE     Comprehensive genomic understanding of coevolution of mcr genes in
            E. coli strains via nanopore sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8197)
  AUTHORS   Li,R., Li,Y., Zhang,P. and Bai,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) College of Veterinary Medicine (Institute of
            Comparative Medicine), Yangzhou University, Wenhui Road, Yangzhou,
            Jiangsu 225009, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2019
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/13/2023 04:03:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,962
            CDSs (total)                      :: 4,844
            Genes (coding)                    :: 4,624
            CDSs (with protein)               :: 4,624
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 220
            CDSs (without protein)            :: 220
            Pseudo Genes (ambiguous residues) :: 0 of 220
            Pseudo Genes (frameshifted)       :: 59 of 220
            Pseudo Genes (incomplete)         :: 160 of 220
            Pseudo Genes (internal stop)      :: 31 of 220
            Pseudo Genes (multiple problems)  :: 30 of 220
            Pseudo Genes (short protein)      :: 2 of 220
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP053727.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8197
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="CP66-6_Sichuan"
                     /isolation_source="slaughterhouse"
                     /host="pig"
                     /db_xref="taxon:562"
                     /plasmid="pCP66-6-IncQ"
                     /geo_loc_name="China: Sichuan"
                     /collection_date="2016-07"
     gene            1..840
                     /locus_tag="HP433_RS24430"
                     /old_locus_tag="HP433_24445"
     CDS             1..840
                     /locus_tag="HP433_RS24430"
                     /old_locus_tag="HP433_24445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="WP_094337857.1"
                     /translation="MATHKPINILEAFTAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVLYLPAEDPPTAIHHRLHALGAYLNDEQRQAVAA
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
                     /name="helicase RepA family protein"
     gene            827..1678
                     /gene="repC"
                     /locus_tag="HP433_RS24435"
                     /old_locus_tag="HP433_24450"
     CDS             827..1678
                     /gene="repC"
                     /locus_tag="HP433_RS24435"
                     /old_locus_tag="HP433_24450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C, IncQ-type"
                     /protein_id="WP_000240536.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
                     /name="repC"
     gene            1973..>2389
                     /locus_tag="HP433_RS24440"
                     /old_locus_tag="HP433_24460"
                     /pseudo=""
     CDS             1973..>2389
                     /locus_tag="HP433_RS24440"
                     /old_locus_tag="HP433_24460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /name="dihydropteroate synthase"
     gene            2747..3769
                     /locus_tag="HP433_RS24445"
                     /old_locus_tag="HP433_24470"
     CDS             2747..3769
                     /locus_tag="HP433_RS24445"
                     /old_locus_tag="HP433_24470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821153.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA]; GO:0008324 - monoatomic cation
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="WP_000083351.1"
                     /translation="MSQSHAHDHGHDHDHDHTPTVTSANERKVLVSFFLIFGFMLVEAV
                     GGVLSGSLALLADAGHMLTDAVALALAYAAFRFGRRAADSKRTFGYLRFEVIAGFLNAV
                     TLFAIVAWIAYEAWERLQAPPVILAGPMMIVAVLGLLINVLVLWIMTRGETDHVNVKGA
                     ILHVMGDLLGSVGAIVAAIVIYFTGWTPIDPILSVLVAALILRSAWKLLAKSIHILLEG
                     APEDASPEKVEQGLMSTVPGLAAVSHVHVWQLTSGRTMATLHVRPNVDEEARAVVKRVE
                     VVLREQFSIEHATVGIDWNSEADENICSLQPTTDRQDHSGHGHSEHNHDHDHSAPGHKH
                     "
                     /name="cation diffusion facilitator family transporter"
     gene            3813..4190
                     /locus_tag="HP433_RS24450"
                     /old_locus_tag="HP433_24475"
     CDS             3813..4190
                     /locus_tag="HP433_RS24450"
                     /old_locus_tag="HP433_24475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821152.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WP_000203935.1"
                     /translation="MTSRKTTSSAIAHCSTSSHGATTDVLALSQSDVAVLAETFRLLGD
                     QSRLKILLQCMRGSVAVGDIADSLDLSQSLVSHHLRLLRGARLVRGERQAKHIFYGIAD
                     QHVSQVLQDMAVHISEDRNDD"
                     /name="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            complement(<4295..4456)
                     /locus_tag="HP433_RS24455"
                     /old_locus_tag="HP433_24480"
                     /pseudo=""
     CDS             complement(<4295..4456)
                     /locus_tag="HP433_RS24455"
                     /old_locus_tag="HP433_24480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(5100..5267)
                     /locus_tag="HP433_RS24460"
                     /old_locus_tag="HP433_24485"
     CDS             complement(5100..5267)
                     /locus_tag="HP433_RS24460"
                     /old_locus_tag="HP433_24485"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_161403145.1"
                     /translation="MTDLKLDDYTDADFIDELLSDELRCECGEELHYYDDGSLSRCNSC
                     DPDRETNPNL"
                     /name="hypothetical protein"
     gene            complement(5294..5578)
                     /locus_tag="HP433_RS24465"
                     /old_locus_tag="HP433_24490"
     CDS             complement(5294..5578)
                     /locus_tag="HP433_RS24465"
                     /old_locus_tag="HP433_24490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="WP_094336938.1"
                     /translation="MAKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPA"
                     /name="mobilization protein"
     gene            5778..>6416
                     /locus_tag="HP433_RS24930"
                     /pseudo=""
     CDS             5778..>6416
                     /locus_tag="HP433_RS24930"
                     /inference="COORDINATES: protein motif:HMM:NF015354.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /name="MobA/MobL family protein"
     gene            6526..6939
                     /locus_tag="HP433_RS24935"
     CDS             6526..6939
                     /locus_tag="HP433_RS24935"
                     /inference="COORDINATES: protein motif:HMM:NF036929.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncQ-type mobilization protein MobB"
                     /protein_id="WP_223384840.1"
                     /translation="MSAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTQQAS
                     EAQAAEWLKAQRETQAAWAKLAKELREVASEVSDAAQSARSAARGWHWKLWLTVMAASM
                     MPTLVLLIASLLLLDLTPLTTEDGSIWLRLVAR"
                     /name="IncQ-type mobilization protein MobB"
     gene            6984..7907
                     /locus_tag="HP433_RS24940"
     CDS             6984..7907
                     /locus_tag="HP433_RS24940"
                     /inference="COORDINATES: protein motif:HMM:NF028103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-primase RepB domain-containing protein"
                     /protein_id="WP_198870443.1"
                     /translation="MGCERFDIGVRDATTGQMMNREWSASEVLQNTPWLKRMNAQGNDV
                     YIRPAEQERHGLVLVDDLSELDLEDMKAEGREPALIVETSPKNYQAWVKVADAAGGELR
                     GQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRTGYQPWVLLRESKGKTATAG
                     PALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALDEYRSEMAGLVKRYGDD
                     LSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEADYIERTVSKVMGLPSVQ
                     LARAELAQAPAPRQRGMDRGGPDFSM"
                     /name="DNA-primase RepB domain-containing protein"
     gene            7963..8169
                     /gene="urfF"
                     /locus_tag="HP433_RS24475"
                     /old_locus_tag="HP433_24500"
     CDS             7963..8169
                     /gene="urfF"
                     /locus_tag="HP433_RS24475"
                     /old_locus_tag="HP433_24500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid repression protein F"
                     /protein_id="WP_094336940.1"
                     /translation="MKDHRDTQTGNLLATPNAIRQARFADAQRKLGRKARKIWATDDEA
                     EALRLYLEELRAAQGGGSDPASA"
                     /name="urfF"
     ARG             1974..2488
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide resistant sul"
                     /tool="rgi"
                     /drug_class="sulfonamide antibiotic"
                     /identity="100.0"
                     /coverage="63.1"
     Typing          5533..5689
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.671"
                     /coverage="100.0"
                     /evalue="1.08e-59"
     Typing          19..840
                     /name="IncQ1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.813"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          5779..7904
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.769"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggctaccc ataagcctat caatattctg gaggcgttca cagcagcgcc gccaccgctg
       61 gactacgttt tgcccaacat ggtggccggt acggtcgggg cgctggtgtc gcccggtggt
      121 gccggtaaat ccatgctggc cctgcaactg gccgcacaga ttgcaggcgg gccggatctg
      181 ctggaggtgg gcgaactgcc caccggcccg gtgctctacc tgcccgccga agatccaccc
      241 accgccatcc atcaccggct tcacgccctg ggcgcatacc tcaacgacga gcagcggcaa
      301 gccgtggccg ctggcctgct gatccagccg ctgatcggca gcctgcccaa catcatggcc
      361 ccggagtggt tcgacggcct caagcgcgcc gccgagggcc gccgcctgat ggtgctggac
      421 acgctgcgcc ggttccacat cgaggaagaa aacgccagcg gccccatggc ccaggtcatc
      481 ggtcgcatgg aggccatcgc cgccgatacc gggtgctcta tcgtgttcct gcaccatgcc
      541 agcaagggcg cggccatgat gggcgcaggc gaccagcagc aggccagccg gggcagctcg
      601 gtactggtcg ataacatccg ctggcagtcc tacctgtcga gcatgaccag cgccgaggcc
      661 gaggaatggg gtgtggacga cgaccagcgc cggttcttcg tccgcttcgg tgtgagcaag
      721 gccaactatg gcgcaccgtt cgctgatcgg tggttcaggc ggcatgacgg cggggtgctc
      781 aagcccgccg tgctggagag gcagcgcaag agcaaggggg tgccccgtgg tgaagcctaa
      841 gaacaagcac agcctcagcc acgtccggca cgacccggcg cactgtctgg cccccggcct
      901 gttccgtgcc ctcaagcggg gcgagcgcaa gcgcagcaag ctggacgtga cgtatgacta
      961 cggcgacggc aagcggatcg agttcagcgg cccggagccg ctgggcgctg atgatctgcg
     1021 catcctgcaa gggctggtgg ccatggctgg gcctaatggc ctagtgcttg gcccggaacc
     1081 caagaccgaa ggcggacggc agctccggct gttcctggaa cccaagtggg aggccgtcac
     1141 cgctgatgcc atggtggtca aaggtagcta tcgggcgctg gcaaaggaaa tcggggcaga
     1201 ggtcgatagt ggtggggcgc tcaagcacat acaggactgc atcgagcgcc tttggaaggt
     1261 atccatcatc gcccagaatg gccgcaagcg gcaggggttt cggctgctgt cggagtacgc
     1321 cagcgacgag gcggacgggc gcctgtacgt ggccctgaac cccttgatcg cgcaggccgt
     1381 catgggtggc ggccagcatg tgcgcatcag catggacgag gtgcgggcgc tggacagcga
     1441 aaccgcccgc ctgctgcacc agcggctgtg tggctggatc gaccccggca aaaccggcaa
     1501 ggcttccata gataccttgt gcggctatgt ctggccgtca gaggccagtg gttcgaccat
     1561 gcgcaagcgc cgccagcggg tgcgcgaggc gttgccggag ctggtcgcgc tgggctggac
     1621 ggtaaccgag ttcgcggcgg gcaagtacga catcacccgg cccaaggcgg caggctgacc
     1681 ccccccactc tattgtaaac aagacatttt tatcttttat attcaatggc ttattttcct
     1741 gctaattggt aataccatga aaaataccat gctcagaaaa ggcttaacaa tattttgaaa
     1801 aattgcctac tgagcgctgc cgcacagctc cataggccgc tttcctggct tatgctattc
     1861 tgctcctgca gctaatggat caccgcaaac aggttactcg cctggggatt ccctttcgac
     1921 ccgagcatcc gtatgagact catgctcgat tattattatt atagaagccc ccatgaataa
     1981 atcgctcatc attttcggca tcgtcaacat aacctcggac agtttctccg atggaggccg
     2041 gtatctggcg ccagacgcag ccattgcgca ggcgcgtaag ctgatggccg agggggcaga
     2101 tgtgatcgac ctcggtccgg catccagcaa tcccgacgcc gcgcctgttt cgtccgacac
     2161 agaaatcgcg cgtatcgcgc cggtgctgga cgcgctcaag gcagatggca ttcccgtctc
     2221 gctcgacagt tatcaacccg cgacgcaagc ctatgccttg tcgcgtggtg tggcctatct
     2281 caatgatatt cgcggttttc cagacgctgc gttctatccg caattggcga aatcatctgc
     2341 caaactcgtc gttatgcatt cggtgcaaga cgggcaggca gatcggcgcg gtcatgggct
     2401 taccatcgac cttcattgtc atgtctctga tcggcttggc cggcgtggtg ctggcgctct
     2461 ggctgtggcc ggccagcgac ccttctgacg tggtgcatga ccaccccagc ttgccgccgg
     2521 atcaccctca tttacgcaca cacgcagacc gaggcaagca ccatcaccag ctgattgtgg
     2581 atgacctgca ccgtatctgg ccgaaaggat aggcacattt tctcgttgcg gcaccacaac
     2641 gtatggcttc ggcggctggg aagggtgagc gaaaatactt cacatgaaca tgtattcatg
     2701 tgttatattt tcaccatagt caaacactca accagcagag gcgcccatgt cccaatccca
     2761 tgcacatgac catggtcacg accatgatca cgaccacacc cccacagtga ccagcgccaa
     2821 cgagcgcaag gttctggttt ccttcttcct gatcttcggc ttcatgctcg tggaagctgt
     2881 cggcggcgtg ctgtcgggct cgctagccct acttgccgat gctggccaca tgctgaccga
     2941 cgccgtggca cttgccctgg cttacgctgc cttccgattc ggtcgccgcg cagccgacag
     3001 caaacgcaca ttcggttacc tgcgcttcga ggtcatcgcc ggcttcctga acgccgtgac
     3061 cctgttcgcg atcgtcgcat ggatcgccta cgaggcgtgg gagcggctgc aggcgccgcc
     3121 cgtcatcctc gccggcccga tgatgatcgt cgccgtgctc ggcttactga tcaacgtcct
     3181 cgtgctgtgg atcatgaccc gcggcgaaac cgatcacgtc aatgtgaaag gtgccattct
     3241 ccacgtgatg ggtgacctgc tgggctctgt cggcgccatc gttgcagcga tcgtcatcta
     3301 tttcaccggc tggacgccga tcgaccccat tctgtcggtc ttggtggcag cgttgattct
     3361 gcgcagcgcc tggaagctcc tggccaagtc gatccatatc ctgctggaag gcgcgccaga
     3421 ggatgcttcg ccggaaaagg tggaacaagg cctgatgagc actgtacccg gcctggcagc
     3481 tgtcagccac gttcatgtgt ggcaactcac ttccggtcgc acgatggcca cactgcacgt
     3541 tcgacccaat gtggacgagg aggcgcgagc cgtggtcaag cgcgtcgagg tggtgcttcg
     3601 agagcaattc agcatcgaac acgccacagt cggaatcgac tggaactcgg aggctgacga
     3661 gaacatctgc agcctgcaac ccacgacaga ccgtcaggac cacagcggcc atggccacag
     3721 tgagcacaat catgaccatg accactccgc tcctggtcac aagcattgag gccgtgcgag
     3781 ccggctcgca gttcacttga gggtttgaat ccatgacatc acgcaaaacc accagctccg
     3841 ctattgctca ctgttcgacc tccagccacg gcgcgaccac tgatgtcctc gcactgtcgc
     3901 aaagtgacgt cgctgttctg gccgagacct tccgcctgct gggcgaccaa tcgcgattga
     3961 aaatcctgtt gcagtgcatg cgcggatccg tcgcagtggg cgacattgcc gattcgctag
     4021 acctgtcgca atccttggtc agccatcacc tgcgcctgtt gcgcggcgcg cgcctcgtgc
     4081 gcggcgagcg ccaggccaag cacatcttct acggcatcgc cgaccagcac gtcagccagg
     4141 tactgcagga catggcggtt cacatttcgg aggacagaaa cgacgattga ctgaacgtgt
     4201 gagtgcgcgc cggcctactc cttcaagggg taggcttttt tttggctatc aatctagaga
     4261 aacaccgacg cgccttgctg aatgcctaga accccacata gcccggcaat tcctttccct
     4321 gctctgccat aagcgcagcg aatgccgggt aatactcgtc aacgatctga tagagaaggg
     4381 tttgctcggg tcggtggctc tggtaacgac cagtatcccg atcccggctg gccgtcctgg
     4441 ccgccacatg aggcatgttc cgcgtccttg caatactgtg tttacataca gtctatcgct
     4501 tagcggaaag ttcttttacc ctcagccgaa atgcctgccg ttgctagaca ttgccagcca
     4561 gtgcccgtca ctcccgtact aactgtcacg aacccctgca ataactgtca cgcccccctg
     4621 caataactgt cacgaacccc tgcaataact gtcacgcccc cctgcaataa ctgtcacgaa
     4681 cccctgcaat aactgtcacg cccccaaacc tgcaaaccca gcaggggcgg gggctggcgg
     4741 ggtgttggaa aaatccatcc atgattatct aagaataatc cactaggcgc ggttatcagc
     4801 gcccttgtgg ggcgctgctg cccttgccca atatgcccgg ccagaggccg gatagctggt
     4861 ctattcgctg cgctaggcta cacaccgccc caccgctgcg cggcaggggg aaaggcgggc
     4921 aaagcccgct aaaccccaca ccaaaccccg cagaaatacg ctggagcgct tttagccgct
     4981 ttagcggcct ttccccctac ccgaagggtg ggggggcgtg tgcagccccg cagggcctgt
     5041 ctcggtcgat cattcagcat cggcatcatc tgctaatttc ccgcaatgtt cgcaggtaat
     5101 cataggtttg gattggtttc tctatctgga tcacacgaat tgcagcgact caatgaacca
     5161 tcatcgtaat aatgaagctc ctctccgcat tcacagcgca gttcatcact gagcagttca
     5221 tcaataaaat cagcgtctgt gtaatcgtct agttttaaat cagtcattat tgctttcctt
     5281 tattagttta aattcaggcc ggctcatcct tctggcgtgg cggcagaccg aacaaggccc
     5341 ggtcgtggtc gcgttcaagg tacgcatcca ttgccgccat gagccgatct tctggccact
     5401 cgctgctgtt caccttggcc agaatcatgg cgcccaccag caccttgcgc cgcgtttcgt
     5461 tcttgcgctc ttgctgctgc tcccttgccc tcacccgctg aatctcggca ttgatccgtg
     5521 ctcgctgttc ttcgagcttg gccagcctat ccgccgcctt gttgctcccc ttagccatct
     5581 tgacacctca ttgttaatgt gctgtctctt aggctatcat ggaggcacga cggcggcaaa
     5641 ccccgaccgt actttgtacg ggagggcgca cttaccggtt tctcttcgag aaactggcct
     5701 aacggccacc cttcgggcgg tgcgctctcc gagggccatt gcatggagcc gaaaagcaaa
     5761 agcaacagcg aggcagcatg gcgatttatc accttacggc gaaaaccggc agcaggtcgg
     5821 gcggccaatc ggccaaggcc aaggccgact acatccagcg cgaaggcaag tatgcccgcg
     5881 acatggatga agtgctgcac gccgaatccg ggcacatgcc ggagttcgtc gagcggcccg
     5941 ccgactactg ggatgctgcc gacctgcatg aacgcgccaa tgggcggctg ttcaaggagg
     6001 tcgaatttgc cctgccggtc gagctgaccc tcgaccagca gaaggcgctg gcgtccgagt
     6061 tcgcccagca cctgaccggt gccgaacgtt tgccgtatac gctggccatc catgccggtg
     6121 gcggcgagaa cccgcactgc cacctgatga tctccgagcg gatcaatgac ggcatcgagc
     6181 ggcccgccgc tcagtggttc aagcggtaca acggcaaggc cccggagaag ggcggagcgc
     6241 agaagaccga agcactcaag cccaaggcat ggcttgagca gacccgcgag gcatgggccg
     6301 accatgccaa ccgggcatta gagcgggctg gccacgacgc ccgcattgac caccgaacgc
     6361 ttgaggcgca gggcatcgag cgcctgcccg gtgttcacct ggggccgaac gtggtggaga
     6421 tggaaggccg gggcattcgc accgaccggg cagacgtggc cctgaacatc gacaccgcca
     6481 acgcccagat catcgacttg caggaatacc gggaggctat agaccatgag cgcaatcgac
     6541 agagtgaaga aatccagagg aatcaacgag ttagcggagc agatcgagcc gctggcccag
     6601 agcatggcga cactggccga cgaggcccgg caggtcatga gccagaccca gcaggccagc
     6661 gaggcgcagg cagcggagtg gctgaaagcc cagcgcgaga cacaggcagc atgggccaag
     6721 ctggccaagg aattgcggga agtggccagc gaggtgagcg acgccgcgca gagcgcccgg
     6781 agcgcggcca gagggtggca ctggaagctg tggctaaccg tgatggcggc ttccatgatg
     6841 cctacgctgg tgctgctgat cgcatcgttg ctcttgctcg acctgacgcc actgacaaca
     6901 gaggacggct cgatctggct gcgcttggtg gcccgatgaa aaacgacaga accttgcagg
     6961 ccataggccg acagctcaag gccatgggct gtgagcgctt cgatatcggc gtcagggatg
     7021 ccaccaccgg ccagatgatg aaccgggaat ggtcagcctc cgaagtgctc cagaacacgc
     7081 catggctcaa gcggatgaat gcccagggca atgacgtgta tatcaggccc gccgagcagg
     7141 aacgccatgg tctggtgctg gtggacgacc tcagcgagct cgacctggag gacatgaaag
     7201 ccgagggccg ggaaccggcg ctgatcgtgg agaccagccc gaagaactat caggcatggg
     7261 tcaaggtggc cgacgccgca ggcggtgaac tccgggggca gattgcccgg acgctggcca
     7321 gcgagtacga cgccgacccg gccagcgccg acagccgcca ctatggccgc ttggcgggtt
     7381 tcaccaaccg caaggacaag cacaccaccc gcaccggcta tcagccgtgg gtgctgctgc
     7441 gtgagtccaa gggcaagacc gccaccgctg gcccggcgct ggtgcagcag gctggccagc
     7501 agatcgagca ggcccagcgg cagcaggaga aggcccgcag gctggccagc ctcgaactgc
     7561 ccgagcggca gcttagccgc caccggcgca cggcgctgga cgagtaccgc agcgagatgg
     7621 ccggactggt caagcgctac ggtgacgacc tcagcaagtg cgactttatc gccgcgcaga
     7681 aactggccag ccggggccgc agtgccgagg aaatcggcaa ggccatggcc gaggccagcc
     7741 cggcgctggc agagcgcaag cccggccacg aagcggatta catcgagcgc accgtcagca
     7801 aggttatggg tctgcccagc gtccagcttg cgcgggccga gctggcacag gcaccggcac
     7861 cccgccagcg aggcatggac aggggcgggc cagatttcag catgtaacgc ttgcattagc
     7921 gctaacgggt agtatactgt tagctctaat ggaggatttg agatgaaaga ccatcgggac
     7981 acgcagaccg gcaacctgtt ggccacgccc aacgcgatca ggcaggctag gtttgccgac
     8041 gctcagcgaa aactgggccg caaggcccgt aagatatggg ccacagacga cgaggccgag
     8101 gcgctgcgtc tgtacctgga agaactcaga gcggcgcagg gcgggggtag tgaccccgcc
     8161 agcgcctaac caccaactgc ctgaacagga ggcaatc
//
