LOCUS       NZ_CP053695             6364 bp    DNA     circular CON 04-APR-2024
DEFINITION  Enterobacter hormaechei strain Eh27 plasmid pEh27_4, complete
            sequence.
ACCESSION   NZ_CP053695
VERSION     NZ_CP053695.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14943915
            Assembly: GCF_023897035.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 6364)
  AUTHORS   Zhou,K.
  TITLE     Carbapenem- and colistin-resistant Enterobacter hormaechei
            co-producing NDM-1 and MCR-9
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6364)
  AUTHORS   Zhou,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) Institute of Respiratory Diseases, Shenzhen
            People's Hospital, Dongmenbeilu, Shenzhen 518020, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 110.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023897035.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 07:08:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,975
            CDSs (total)                      :: 4,856
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 26 of 89
            Pseudo Genes (incomplete)         :: 68 of 89
            Pseudo Genes (internal stop)      :: 13 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            Pseudo Genes (short protein)      :: 1 of 89
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP053695.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6364
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="Eh27"
                     /isolation_source="Deep venous catheter"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pEh27_4"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="22.5575 N 114.129 E"
                     /collection_date="2013-05-28"
                     /collected_by="Shenzhen People's Hospital"
     gene            126..584
                     /locus_tag="HPC66_RS24365"
                     /old_locus_tag="HPC66_24305"
     CDS             126..584
                     /locus_tag="HPC66_RS24365"
                     /old_locus_tag="HPC66_24305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005119689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032635016.1"
                     /translation="MAWHTVKDTLKLTGRSRSQLYRDMKKGLVSYRTGKDGRREFDTSE
                     LIRAYGELRTEETVKRHKKGQQEIDDTPAEHPQLKEIQQQLTALQQTVTLMLEDKSTRE
                     AELRQHDEERTQLLAEISRLNEDLEQEKKRSLWSRLFRRDIEEKNREK"
                     /name="hypothetical protein"
     gene            990..1412
                     /gene="tolA"
                     /locus_tag="HPC66_RS24370"
                     /old_locus_tag="HPC66_24310"
     CDS             990..1412
                     /gene="tolA"
                     /locus_tag="HPC66_RS24370"
                     /old_locus_tag="HPC66_24310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014063821.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_023316030.1"
                     /translation="MPLSVRFSPFMFFIPLFLTACTASPHTSVEKSAKAETTQPVDDLL
                     GDLKAGSSDNVASYATKIKHAVEEKMYKPESYKEQKCTIRLLLGRDGLVMGATAESGDS
                     ELCNAVMMAVKQANIPPAPDEKTWQEFRNTSLDFSY"
                     /name="tolA"
     gene            complement(1532..1747)
                     /locus_tag="HPC66_RS24375"
                     /old_locus_tag="HPC66_24315"
     CDS             complement(1532..1747)
                     /locus_tag="HPC66_RS24375"
                     /old_locus_tag="HPC66_24315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_045898725.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     MLSERVSDLSRQVQSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1754..2245)
                     /locus_tag="HPC66_RS24380"
                     /old_locus_tag="HPC66_24320"
     CDS             complement(1754..2245)
                     /locus_tag="HPC66_RS24380"
                     /old_locus_tag="HPC66_24320"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_139154006.1"
                     /translation="MSSLLTLAKDLEQQSKAQQKSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAISAHEQSMNEAMEKNRRSVLLTAGRAWLTILMVSALLIATSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTSRTWGVRYQESSDGRRFLILPPGMQAEAIPYNGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            complement(<2280..2984)
                     /locus_tag="HPC66_RS24905"
                     /old_locus_tag="HPC66_24325"
                     /pseudo=""
     CDS             complement(<2280..2984)
                     /locus_tag="HPC66_RS24905"
                     /old_locus_tag="HPC66_24325"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2923..3246)
                     /locus_tag="HPC66_RS24390"
                     /old_locus_tag="HPC66_24330"
     CDS             complement(2923..3246)
                     /locus_tag="HPC66_RS24390"
                     /old_locus_tag="HPC66_24330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_180264886.1"
                     /translation="MLTMWVTEDEHRRLLERCEGRQLAAWMRQTCLDEKPARTGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGMD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3894..4028
                     /locus_tag="HPC66_RS24910"
     CDS             3894..4028
                     /locus_tag="HPC66_RS24910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048230020.1"
                     /translation="MHEPPVQSDCCACSGNYRLESNPERHEKTPLAAATGKQVVREMY"
                     /name="hypothetical protein"
     gene            complement(4231..4380)
                     /locus_tag="HPC66_RS24395"
                     /old_locus_tag="HPC66_24340"
     CDS             complement(4231..4380)
                     /locus_tag="HPC66_RS24395"
                     /old_locus_tag="HPC66_24340"
                     /inference="COORDINATES: protein motif:HMM:NF014457.4"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_071992647.1"
                     /translation="MRISFFSGIVIVSMILAACQVNNVRDTGGGTVSPTSTVTGVTLDS
                     GTNR"
                     /name="colicin release lysis protein"
     gene            4506..4859
                     /locus_tag="HPC66_RS24400"
                     /old_locus_tag="HPC66_24345"
     CDS             4506..4859
                     /locus_tag="HPC66_RS24400"
                     /old_locus_tag="HPC66_24345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071992646.1"
                     /translation="MTIKYYLKHCLWGLIGCGYLTYLILTDLNSGDIYPAYAPYMPYIA
                     SYLAVSAVIFPFAFYTTEQLSLKKMKRETWGNYFGSDSPSWGAFIFVYIFCVLLSVPLF
                     FIYPFINKNRPGN"
                     /name="hypothetical protein"
     gene            complement(4879..6204)
                     /locus_tag="HPC66_RS24405"
                     /old_locus_tag="HPC66_24350"
     CDS             complement(4879..6204)
                     /locus_tag="HPC66_RS24405"
                     /old_locus_tag="HPC66_24350"
                     /inference="COORDINATES: protein motif:HMM:NF013211.4"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0140911 - pore-forming activity [Evidence
                     IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA];
                     GO:0050829 - defense response to Gram-negative bacterium
                     [Evidence IEA]; GO:0031640 - killing of cells of another
                     organism [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="WP_096889074.1"
                     /translation="MPGFNYGGNSVGDGTNWSSERGSDPTTPGGGDNGHAGDHSSSGSS
                     SSTVEHTPWGDLTTDAAGYAYMNGIKLTADNSQLAYVSPTHLVRVLNSLLDRSYMKPEP
                     GEHSGVTGVPATSPSRVTLADNALKNAQSKAQDAAAALSKANAAISQAQTRLNTAKAKI
                     PSLEANVNTKQQASDKATARAHELAHFLNDMGGPGRYNALNAGYQSEVAQSELDNAKKA
                     LANGHSEITAAQKALDDAKKALPNATANKQKADSAVASAADMKDAITTTTAFFESISKK
                     FGERAARLAQELAAQARSAKIRSVPEAVALFDKHKGTAVNKLNAADKAAIANAFKSLDQ
                     AAMAKQLAAYSKVLGHFNKAMDFADLGAAVKKGIETGNWNDAINKLESIALTNTAGQLA
                     AIAFAAAGAPLGIVGFGIVMTLSGVLFSNEKVLSAINTSLGM"
                     /name="colicin-like pore-forming protein"
     Typing          complement(3360..3532)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="89.773"
                     /coverage="100.0"
                     /evalue="9.61e-56"
     Typing          3499..4021
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.847"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(1441..2933)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.4"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 acatcgtcaa catgattttt ttgtggtttt ttcacttcct gcggacaacc ttagatatgc
       61 catgttaaaa cagaaatata atctgcgagc tcaacatgag acatacagga gacacggtaa
      121 tttttatggc ttggcacacc gtaaaggaca cactaaaact gaccggtcgt tcgcgatccc
      181 agctgtatcg ggacatgaaa aaaggccttg tcagttaccg cacagggaaa gatggacggc
      241 gagaattcga caccagcgag ctgattcggg cttacggtga actgcggacg gaggagacag
      301 tcaaaaggca caaaaaggga cagcaagaaa ttgacgatac tccagctgaa cacccgcaac
      361 tgaaagagat acaacagcag ttaactgccc tgcagcagac ggtcacattg atgttggagg
      421 ataaaagcac tcgcgaagcc gaactccggc aacacgatga ggagcggaca cagctgctgg
      481 ccgaaatcag ccgactgaat gaagatctgg agcaggaaaa aaaacgcagc ctctggtcca
      541 ggttgttccg acgtgatatt gaggagaaaa atcgtgaaaa atgacagttt ggagaacgtc
      601 attcatgaag cggatacacg ggagaagcat ctgttcagat actggcgatg gtgtctgaag
      661 tgggggattt ggtttatgct ggcgttgtac ttgctctggt gctatgtcac cagctgctga
      721 caaacgattt accataacgt ctcattacgc gcaccgctca gacggcctga gggcatttcc
      781 ggcggctacg ggataaaaca ggcaacaaac cacccggaaa accgccagaa acgcacctgc
      841 caggttttat cggcatgcat gactatgcag aaagatgcat gattcttaat ctgacgggaa
      901 acggatggcg agagcgtgat ttctgatagt ttactttggt ttaaccacag aattatgatg
      961 gctcctttat gttaacaagg aatgccgcga tgcctttatc agtacgcttt tcaccattta
     1021 tgttctttat cccgctgttt ttgacggcat gcactgcctc tccacataca agcgtagaaa
     1081 aatcggcaaa agcagaaaca acccagcccg ttgacgacct gctaggggat ttaaaagcag
     1141 gttcaagcga taatgtcgcc agttatgcaa caaaaataaa acacgccgtc gaagaaaaga
     1201 tgtacaaacc tgaatcatat aaggagcaga aatgtactat tcggctctta ctcgggcgcg
     1261 atggtttagt tatgggcgca acagcggaga gcggggactc agagctttgt aacgcagtaa
     1321 tgatggctgt taaacaagcg aatattcctc ctgcaccgga tgaaaaaact tggcaggaat
     1381 tcagaaatac ctctctagat ttttcatact gaaagatttc ccctgctgga ggtcagtcac
     1441 agtgtcggtc cccggtaggt tttctgcggc tgccgctcca gctccagtgt tttctgatac
     1501 tgattatctc tctgccggcg ctcgatggca ttcagttccc ggacaagcgg cgcagctgct
     1561 cgctcaaact ctgcacctgc ctgctcaagt ccgacacgcg ctcgctcagc atcgcgttct
     1621 cccgttgcat aagaccacac atgctccgcc attccgcgaa ggcgctctcc cactcgtcca
     1681 gcctttgcga gtagtcctgc tgtagctgct ctaatgcgct cagcaactgc ttttccagct
     1741 ctgtcatttt ccgctactcc tgtttcaggc ggatccaggt cgtcccgttg taggggatgg
     1801 cttcggcttg catccccggc ggcaggataa ggaacctccg gccgtcgctg ctctcctgat
     1861 agcgtacccc ccacgtccgg gaagtcattt tcgccagggt atcttcctgc tgacgaagat
     1921 gcgtgtaatt gtcggttatc tgctgcccct gccaccacag aatgctcccg ctcgtggcga
     1981 tcagcagggc tgacaccatc aggatggtga gccatgcccg cccggccgta agcagcacgc
     2041 tccgccggtt tttctccatc gcctcgttca tactctgttc gtgggcgctg atggcgtcgc
     2101 tgattctcct cgcgcttgcg ctcagttccg ctctgacaga ctgctcgtgc tcgctgaatg
     2161 cggctttcag catctcgccg gtactcttct gctgcgcttt cgattgctgc tctaagtcct
     2221 tcgccagcgt taaaagactg ctcatagatg gctcccttca gtcggatgtt tcgccccccg
     2281 tcagggtcgg caatgctgat actgcttttc gtggtgcgta ccacctcaaa acctgaccgc
     2341 tcaagcgcct cagtgacgtc atgacggctt ttcagctccc cggacgaggc caagctcagc
     2401 agccccctgg tgatggcttc tgcggcatcc tgcttcgttt tcggcagccc ggccggcgtg
     2461 accagcgccc gacggttttc cggcgcgttc gggtcgagca gccccagccg gccgttgacc
     2521 accgtctgcc aggcatcgat gcgtggccgg tcggcgcggt catagtacgg ctggagccgc
     2581 ctgccggtca gcagctcggt attggggatc aggaagttca gctccagacg ccccttgtcc
     2641 gcgtgctcca cccacagaat gctgtactga tctttatcga gtccgggcat cagaacccgc
     2701 tcgaagctgg ccatcagttt ttcccgctgg ccgggaggca gatcggcctc cgcaaacgac
     2761 agtacgccgg aggtgtattt cttcacgtaa ggcgacgcat caatcagctc ccggacttct
     2821 tccggcttgc cctgcaggac ggtggcgcct tcacgctgcc ggtctttacc cagcagatag
     2881 tccaccggcc cggcgccacc gccgcggccg cgcggatgaa atttaacgat catcgtccat
     2941 tcctttctca agaacggcgt gccgcagccg ctcaaggccg gcatcgatgg ccatcagcgc
     3001 cgccacaatc tgcaccctgt catggccggt accgccgccg gcgttaacgc ggcgggctat
     3061 ctggttcagg ttgttgccca tgccggcaag ctggcgcagc agcgccggcg agatcgaggg
     3121 aagctttccg gtacgcgcag gcttttcgtc caggcaggtc tgccgcatcc acgctgccag
     3181 ctgcctgccc tcgcagcgct cgagcagccg ccggtgctcg tcctccgtca cccacatggt
     3241 gagcatcttg ttccgtttct ctgacaaacc ggtctcctga taacgccggg acgggcggga
     3301 aaacctttcc cggtcggcgc ggggttggac aagccgcagg cgcgtcaggc ttttggcggg
     3361 tttcggggcg cagccctgaa ccagtcacgt agcgctagcg gagtgtatac tggcttaata
     3421 tgttacggcg gagtaacact ttatgaaagt gcttcatgta gcaggagaaa aaggtggcac
     3481 cagcgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc actgactcgc
     3541 tacgctcggt cgttcggctg cggcgagcgg aaatggctta cgaacggggc ggaaatttcc
     3601 tggaagatgc caggaagaaa cttaacaggg aagcgagaag gccacggcgg agccgttttt
     3661 ccataggctc cgcccccctg acaagcatca cagaatctga cgctcaaatc agtggtggcg
     3721 aaacccgaca ggactataaa gatcccaggc gtttccccct ggtagctccc tcgtgcgctc
     3781 tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc gtttatctca
     3841 ttccacgcct gacactcggt tccgggaagg cagttcgctc caagctggac tgtatgcacg
     3901 aaccccccgt tcagtccgac tgctgcgcct gttccggtaa ctatcgactt gagtccaacc
     3961 cggaaagaca cgaaaaaacg ccactggcag cagccactgg taagcaggtt gtgcgagaga
     4021 tgtattgaga gtacttgaag tgatggccag actgcggcta cactgaaagg acagatttgg
     4081 tgacatgtct cgcacaagac tgttacctgg ttcagaaact tgccggtttc tgatccttcg
     4141 aaaaaccacc ttgccgggtg gttttttcgt ttacagagca agagattacg actcatcgca
     4201 aaagatctca agcagatcct ttatggatcg ttacctattc gtaccactgt cgagcgtaac
     4261 gcccgttact gtggatgttg gagaaactgt accacctcca gtatctcgga cgttattcac
     4321 ttggcatgct gcgagaatca tcgatactat gacgattccc gagaaaaaag atattcgcat
     4381 attctatcct tctttttatt attttaggct acatttataa taactttcgg tcttacaatt
     4441 catccattgt ccaaagacag ttttttagat aaaatcaaga atgcatttca acttaaacaa
     4501 aaaaaatgac tattaaatat tacttaaaac attgtctctg gggtttgatt ggctgcggat
     4561 atcttactta cttaatcttg acagatttaa atagtggaga catttatcca gcttatgcgc
     4621 cttatatgcc ttacatagct agctacttag cggtaagtgc agtcatcttc ccttttgctt
     4681 tttacaccac agaacaatta tcattaaaaa aaatgaaaag agaaacatgg ggtaattatt
     4741 tcggttcaga cagcccatct tggggtgctt tcatttttgt ttatattttt tgcgtactgt
     4801 tatcagttcc actgtttttt atttatcctt tcattaataa aaaccgtccg ggtaactagc
     4861 accccggacg gaaactggct acatgcctaa cgatgtatta attgcactca aaactttttc
     4921 gttactaaat aagacaccag acagggtcat gacaatacca aagcccacaa tcccaagtgg
     4981 cgcacctgct gccgcaaaag caatcgcagc taactgtcca gccgtattag ttaaagcaat
     5041 agattcaagc ttgttgatgg catcgttcca attacctgtc tcaatacctt tttttaccgc
     5101 cgcacctaaa tcagcaaaat ccatcgcctt attaaagtgc cctaaaacct ttgaatacgc
     5161 ggccaattgt tttgccatgg ctgcctgatc gagagattta aacgcattcg cgatcgccgc
     5221 cttatctgct gcgttcagtt tgtttacagc agtaccctta tgtttatcaa acaaagcaac
     5281 cgcctcaggc acactacgga tttttgcact acgagcctgg gcagccagtt cctgcgcaag
     5341 gcgagcagcc cgttcaccga attttttcga aattgactcg aaaaaggctg tagtagtggt
     5401 aatcgcatct ttcatatcag cagctgatgc cacagcactg tcagccttct gtttgtttgc
     5461 tgtggcatta ggtaaagcct ttttagcatc atccaaagct ttctgtgcag cggtaatttc
     5521 agaatggcca tttgctaacg cctttttagc gttatcgagc tcactctgtg caacttcaga
     5581 ctgataaccg gcattcaagg cattataacg cccgggccca cccatatcat tcaggaaatg
     5641 agccagctca tgcgcacgag cagtggcctt atctgaagcc tgttgcttcg tgttaacatt
     5701 ggcttcaaga ctagggattt tcgcttttgc tgtattcaga cgtgtttgcg cctgactaat
     5761 agccgcattt gctttcgaca gagcagctgc ggcatcttgg gcttttgatt gtgcattctt
     5821 cagcgcgtta tctgcgaggg ttacacggct tggggatgtc gcaggcaccc ccgtcacacc
     5881 ggaatgttca cctggctcgg gtttcatgta agaacgatcg agcagagaat taagtactcg
     5941 cacaagatga gttggagaaa cgtaagcaag ttgcgagtta tctgccgtca gtttaatacc
     6001 attcatataa gcatagccgg cagcgtcggt tgtcaggtct ccccaagggg tatgttcaac
     6061 agttgatgac gaactaccag atgaagaatg atctccagca tgaccattat ctccgccccc
     6121 tggcgtagtg gggtcagaac cccgctcaga actccaatta gtaccatccc ctacactgtt
     6181 accaccataa ttaaacccag gcataatttt tccctttttg ttttattttt attagaaatc
     6241 aggtgtgcgg tttgttgcct gccgtttccg cagcgcgctg ttataagtac tcttccacac
     6301 cataatactg tatatataac aatgtgttta tatacagtaa atctaatact acgcccgaaa
     6361 aaag
//
