LOCUS       NZ_CP053647             6364 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain Eh65 plasmid pEh65_4, complete
            sequence.
ACCESSION   NZ_CP053647
VERSION     NZ_CP053647.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14943921
            Assembly: GCF_023547485.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 6364)
  AUTHORS   Zhou,K.
  TITLE     Carbapenem- and colistin-resistant Enterobacter hormaechei
            co-producing NDM-1 and MCR-9
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6364)
  AUTHORS   Zhou,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) Institute of Respiratory Diseases, Shenzhen
            People's Hospital, Dongmenbeilu, Shenzhen 518020, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 110.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023547485.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 22:02:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,998
            CDSs (total)                      :: 4,879
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 28 of 95
            Pseudo Genes (incomplete)         :: 72 of 95
            Pseudo Genes (internal stop)      :: 13 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            Pseudo Genes (short protein)      :: 1 of 95
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP053647.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6364
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="Eh65"
                     /isolation_source="Bronchial"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pEh65_4"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="22.5575 N 114.1286 E"
                     /collection_date="2017-09-25"
                     /collected_by="Shenzhen People's Hospital"
     gene            complement(join(6339..6364,1..297))
                     /locus_tag="HPC69_RS25020"
                     /old_locus_tag="HPC69_24425"
                     /pseudo=""
     CDS             complement(join(6339..6364,1..297))
                     /locus_tag="HPC69_RS25020"
                     /old_locus_tag="HPC69_24425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            945..1079
                     /locus_tag="HPC69_RS25025"
     CDS             945..1079
                     /locus_tag="HPC69_RS25025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048230020.1"
                     /translation="MHEPPVQSDCCACSGNYRLESNPERHEKTPLAAATGKQVVREMY"
                     /name="hypothetical protein"
     gene            complement(1282..1431)
                     /locus_tag="HPC69_RS24490"
                     /old_locus_tag="HPC69_24435"
     CDS             complement(1282..1431)
                     /locus_tag="HPC69_RS24490"
                     /old_locus_tag="HPC69_24435"
                     /inference="COORDINATES: protein motif:HMM:NF014457.4"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_071992647.1"
                     /translation="MRISFFSGIVIVSMILAACQVNNVRDTGGGTVSPTSTVTGVTLDS
                     GTNR"
                     /name="colicin release lysis protein"
     gene            1557..1910
                     /locus_tag="HPC69_RS24495"
                     /old_locus_tag="HPC69_24440"
     CDS             1557..1910
                     /locus_tag="HPC69_RS24495"
                     /old_locus_tag="HPC69_24440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071992646.1"
                     /translation="MTIKYYLKHCLWGLIGCGYLTYLILTDLNSGDIYPAYAPYMPYIA
                     SYLAVSAVIFPFAFYTTEQLSLKKMKRETWGNYFGSDSPSWGAFIFVYIFCVLLSVPLF
                     FIYPFINKNRPGN"
                     /name="hypothetical protein"
     gene            complement(1930..3255)
                     /locus_tag="HPC69_RS24500"
                     /old_locus_tag="HPC69_24445"
     CDS             complement(1930..3255)
                     /locus_tag="HPC69_RS24500"
                     /old_locus_tag="HPC69_24445"
                     /inference="COORDINATES: protein motif:HMM:NF013211.4"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0140911 - pore-forming activity [Evidence
                     IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA];
                     GO:0050829 - defense response to Gram-negative bacterium
                     [Evidence IEA]; GO:0031640 - killing of cells of another
                     organism [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="WP_096889074.1"
                     /translation="MPGFNYGGNSVGDGTNWSSERGSDPTTPGGGDNGHAGDHSSSGSS
                     SSTVEHTPWGDLTTDAAGYAYMNGIKLTADNSQLAYVSPTHLVRVLNSLLDRSYMKPEP
                     GEHSGVTGVPATSPSRVTLADNALKNAQSKAQDAAAALSKANAAISQAQTRLNTAKAKI
                     PSLEANVNTKQQASDKATARAHELAHFLNDMGGPGRYNALNAGYQSEVAQSELDNAKKA
                     LANGHSEITAAQKALDDAKKALPNATANKQKADSAVASAADMKDAITTTTAFFESISKK
                     FGERAARLAQELAAQARSAKIRSVPEAVALFDKHKGTAVNKLNAADKAAIANAFKSLDQ
                     AAMAKQLAAYSKVLGHFNKAMDFADLGAAVKKGIETGNWNDAINKLESIALTNTAGQLA
                     AIAFAAAGAPLGIVGFGIVMTLSGVLFSNEKVLSAINTSLGM"
                     /name="colicin-like pore-forming protein"
     gene            3542..4000
                     /locus_tag="HPC69_RS24505"
                     /old_locus_tag="HPC69_24450"
     CDS             3542..4000
                     /locus_tag="HPC69_RS24505"
                     /old_locus_tag="HPC69_24450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005119689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032635016.1"
                     /translation="MAWHTVKDTLKLTGRSRSQLYRDMKKGLVSYRTGKDGRREFDTSE
                     LIRAYGELRTEETVKRHKKGQQEIDDTPAEHPQLKEIQQQLTALQQTVTLMLEDKSTRE
                     AELRQHDEERTQLLAEISRLNEDLEQEKKRSLWSRLFRRDIEEKNREK"
                     /name="hypothetical protein"
     gene            4406..4828
                     /gene="tolA"
                     /locus_tag="HPC69_RS24510"
                     /old_locus_tag="HPC69_24455"
     CDS             4406..4828
                     /gene="tolA"
                     /locus_tag="HPC69_RS24510"
                     /old_locus_tag="HPC69_24455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014063821.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_023316030.1"
                     /translation="MPLSVRFSPFMFFIPLFLTACTASPHTSVEKSAKAETTQPVDDLL
                     GDLKAGSSDNVASYATKIKHAVEEKMYKPESYKEQKCTIRLLLGRDGLVMGATAESGDS
                     ELCNAVMMAVKQANIPPAPDEKTWQEFRNTSLDFSY"
                     /name="tolA"
     gene            complement(4948..5163)
                     /locus_tag="HPC69_RS24515"
                     /old_locus_tag="HPC69_24460"
     CDS             complement(4948..5163)
                     /locus_tag="HPC69_RS24515"
                     /old_locus_tag="HPC69_24460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_045898725.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     MLSERVSDLSRQVQSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(5170..5661)
                     /locus_tag="HPC69_RS24520"
                     /old_locus_tag="HPC69_24465"
     CDS             complement(5170..5661)
                     /locus_tag="HPC69_RS24520"
                     /old_locus_tag="HPC69_24465"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_139154006.1"
                     /translation="MSSLLTLAKDLEQQSKAQQKSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAISAHEQSMNEAMEKNRRSVLLTAGRAWLTILMVSALLIATSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTSRTWGVRYQESSDGRRFLILPPGMQAEAIPYNGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            complement(<5696..6349)
                     /locus_tag="HPC69_RS25030"
                     /pseudo=""
     CDS             complement(<5696..6349)
                     /locus_tag="HPC69_RS25030"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     Typing          complement(411..583)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="89.773"
                     /coverage="100.0"
                     /evalue="9.61e-56"
     Typing          550..1072
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.847"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(2..297)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.929"
                     /coverage="93.0"
                     /evalue="1.87e-56"
     Typing          complement(4857..6349)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.4"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aagaacggcg tgccgcagcc gctcaaggcc ggcatcgatg gccatcagcg ccgccacaat
       61 ctgcaccctg tcatggccgg taccgccgcc ggcgttaacg cggcgggcta tctggttcag
      121 gttgttgccc atgccggcaa gctggcgcag cagcgccggc gagatcgagg gaagctttcc
      181 ggtacgcgca ggcttttcgt ccaggcaggt ctgccgcatc cacgctgcca gctgcctgcc
      241 ctcgcagcgc tcgagcagcc gccggtgctc gtcctccgtc acccacatgg tgagcatctt
      301 gttccgtttc tctgacaaac cggtctcctg ataacgccgg gacgggcggg aaaacctttc
      361 ccggtcggcg cggggttgga caagccgcag gcgcgtcagg cttttggcgg gtttcggggc
      421 gcagccctga accagtcacg tagcgctagc ggagtgtata ctggcttaat atgttacggc
      481 ggagtaacac tttatgaaag tgcttcatgt agcaggagaa aaaggtggca ccagcgcgtc
      541 agcagaatat gtgatacagg atatattccg cttcctcgct cactgactcg ctacgctcgg
      601 tcgttcggct gcggcgagcg gaaatggctt acgaacgggg cggaaatttc ctggaagatg
      661 ccaggaagaa acttaacagg gaagcgagaa ggccacggcg gagccgtttt tccataggct
      721 ccgcccccct gacaagcatc acagaatctg acgctcaaat cagtggtggc gaaacccgac
      781 aggactataa agatcccagg cgtttccccc tggtagctcc ctcgtgcgct ctcctgttcc
      841 tgcctttcgg tttaccggtg tcattccgct gttatggccg cgtttatctc attccacgcc
      901 tgacactcgg ttccgggaag gcagttcgct ccaagctgga ctgtatgcac gaaccccccg
      961 ttcagtccga ctgctgcgcc tgttccggta actatcgact tgagtccaac ccggaaagac
     1021 acgaaaaaac gccactggca gcagccactg gtaagcaggt tgtgcgagag atgtattgag
     1081 agtacttgaa gtgatggcca gactgcggct acactgaaag gacagatttg gtgacatgtc
     1141 tcgcacaaga ctgttacctg gttcagaaac ttgccggttt ctgatccttc gaaaaaccac
     1201 cttgccgggt ggttttttcg tttacagagc aagagattac gactcatcgc aaaagatctc
     1261 aagcagatcc tttatggatc gttacctatt cgtaccactg tcgagcgtaa cgcccgttac
     1321 tgtggatgtt ggagaaactg taccacctcc agtatctcgg acgttattca cttggcatgc
     1381 tgcgagaatc atcgatacta tgacgattcc cgagaaaaaa gatattcgca tattctatcc
     1441 ttctttttat tattttaggc tacatttata ataactttcg gtcttacaat tcatccattg
     1501 tccaaagaca gttttttaga taaaatcaag aatgcatttc aacttaaaca aaaaaaatga
     1561 ctattaaata ttacttaaaa cattgtctct ggggtttgat tggctgcgga tatcttactt
     1621 acttaatctt gacagattta aatagtggag acatttatcc agcttatgcg ccttatatgc
     1681 cttacatagc tagctactta gcggtaagtg cagtcatctt cccttttgct ttttacacca
     1741 cagaacaatt atcattaaaa aaaatgaaaa gagaaacatg gggtaattat ttcggttcag
     1801 acagcccatc ttggggtgct ttcatttttg tttatatttt ttgcgtactg ttatcagttc
     1861 cactgttttt tatttatcct ttcattaata aaaaccgtcc gggtaactag caccccggac
     1921 ggaaactggc tacatgccta acgatgtatt aattgcactc aaaacttttt cgttactaaa
     1981 taagacacca gacagggtca tgacaatacc aaagcccaca atcccaagtg gcgcacctgc
     2041 tgccgcaaaa gcaatcgcag ctaactgtcc agccgtatta gttaaagcaa tagattcaag
     2101 cttgttgatg gcatcgttcc aattacctgt ctcaatacct ttttttaccg ccgcacctaa
     2161 atcagcaaaa tccatcgcct tattaaagtg ccctaaaacc tttgaatacg cggccaattg
     2221 ttttgccatg gctgcctgat cgagagattt aaacgcattc gcgatcgccg ccttatctgc
     2281 tgcgttcagt ttgtttacag cagtaccctt atgtttatca aacaaagcaa ccgcctcagg
     2341 cacactacgg atttttgcac tacgagcctg ggcagccagt tcctgcgcaa ggcgagcagc
     2401 ccgttcaccg aattttttcg aaattgactc gaaaaaggct gtagtagtgg taatcgcatc
     2461 tttcatatca gcagctgatg ccacagcact gtcagccttc tgtttgtttg ctgtggcatt
     2521 aggtaaagcc tttttagcat catccaaagc tttctgtgca gcggtaattt cagaatggcc
     2581 atttgctaac gcctttttag cgttatcgag ctcactctgt gcaacttcag actgataacc
     2641 ggcattcaag gcattataac gcccgggccc acccatatca ttcaggaaat gagccagctc
     2701 atgcgcacga gcagtggcct tatctgaagc ctgttgcttc gtgttaacat tggcttcaag
     2761 actagggatt ttcgcttttg ctgtattcag acgtgtttgc gcctgactaa tagccgcatt
     2821 tgctttcgac agagcagctg cggcatcttg ggcttttgat tgtgcattct tcagcgcgtt
     2881 atctgcgagg gttacacggc ttggggatgt cgcaggcacc cccgtcacac cggaatgttc
     2941 acctggctcg ggtttcatgt aagaacgatc gagcagagaa ttaagtactc gcacaagatg
     3001 agttggagaa acgtaagcaa gttgcgagtt atctgccgtc agtttaatac cattcatata
     3061 agcatagccg gcagcgtcgg ttgtcaggtc tccccaaggg gtatgttcaa cagttgatga
     3121 cgaactacca gatgaagaat gatctccagc atgaccatta tctccgcccc ctggcgtagt
     3181 ggggtcagaa ccccgctcag aactccaatt agtaccatcc cctacactgt taccaccata
     3241 attaaaccca ggcataattt ttcccttttt gttttatttt tattagaaat caggtgtgcg
     3301 gtttgttgcc tgccgtttcc gcagcgcgct gttataagta ctcttccaca ccataatact
     3361 gtatatataa caatgtgttt atatacagta aatctaatac tacgcccgaa aaaagcacat
     3421 cgtcaacatg atttttttgt ggttttttca cttcctgcgg acaaccttag atatgccatg
     3481 ttaaaacaga aatataatct gcgagctcaa catgagacat acaggagaca cggtaatttt
     3541 tatggcttgg cacaccgtaa aggacacact aaaactgacc ggtcgttcgc gatcccagct
     3601 gtatcgggac atgaaaaaag gccttgtcag ttaccgcaca gggaaagatg gacggcgaga
     3661 attcgacacc agcgagctga ttcgggctta cggtgaactg cggacggagg agacagtcaa
     3721 aaggcacaaa aagggacagc aagaaattga cgatactcca gctgaacacc cgcaactgaa
     3781 agagatacaa cagcagttaa ctgccctgca gcagacggtc acattgatgt tggaggataa
     3841 aagcactcgc gaagccgaac tccggcaaca cgatgaggag cggacacagc tgctggccga
     3901 aatcagccga ctgaatgaag atctggagca ggaaaaaaaa cgcagcctct ggtccaggtt
     3961 gttccgacgt gatattgagg agaaaaatcg tgaaaaatga cagtttggag aacgtcattc
     4021 atgaagcgga tacacgggag aagcatctgt tcagatactg gcgatggtgt ctgaagtggg
     4081 ggatttggtt tatgctggcg ttgtacttgc tctggtgcta tgtcaccagc tgctgacaaa
     4141 cgatttacca taacgtctca ttacgcgcac cgctcagacg gcctgagggc atttccggcg
     4201 gctacgggat aaaacaggca acaaaccacc cggaaaaccg ccagaaacgc acctgccagg
     4261 ttttatcggc atgcatgact atgcagaaag atgcatgatt cttaatctga cgggaaacgg
     4321 atggcgagag cgtgatttct gatagtttac tttggtttaa ccacagaatt atgatggctc
     4381 ctttatgtta acaaggaatg ccgcgatgcc tttatcagta cgcttttcac catttatgtt
     4441 ctttatcccg ctgtttttga cggcatgcac tgcctctcca catacaagcg tagaaaaatc
     4501 ggcaaaagca gaaacaaccc agcccgttga cgacctgcta ggggatttaa aagcaggttc
     4561 aagcgataat gtcgccagtt atgcaacaaa aataaaacac gccgtcgaag aaaagatgta
     4621 caaacctgaa tcatataagg agcagaaatg tactattcgg ctcttactcg ggcgcgatgg
     4681 tttagttatg ggcgcaacag cggagagcgg ggactcagag ctttgtaacg cagtaatgat
     4741 ggctgttaaa caagcgaata ttcctcctgc accggatgaa aaaacttggc aggaattcag
     4801 aaatacctct ctagattttt catactgaaa gatttcccct gctggaggtc agtcacagtg
     4861 tcggtccccg gtaggttttc tgcggctgcc gctccagctc cagtgttttc tgatactgat
     4921 tatctctctg ccggcgctcg atggcattca gttcccggac aagcggcgca gctgctcgct
     4981 caaactctgc acctgcctgc tcaagtccga cacgcgctcg ctcagcatcg cgttctcccg
     5041 ttgcataaga ccacacatgc tccgccattc cgcgaaggcg ctctcccact cgtccagcct
     5101 ttgcgagtag tcctgctgta gctgctctaa tgcgctcagc aactgctttt ccagctctgt
     5161 cattttccgc tactcctgtt tcaggcggat ccaggtcgtc ccgttgtagg ggatggcttc
     5221 ggcttgcatc cccggcggca ggataaggaa cctccggccg tcgctgctct cctgatagcg
     5281 taccccccac gtccgggaag tcattttcgc cagggtatct tcctgctgac gaagatgcgt
     5341 gtaattgtcg gttatctgct gcccctgcca ccacagaatg ctcccgctcg tggcgatcag
     5401 cagggctgac accatcagga tggtgagcca tgcccgcccg gccgtaagca gcacgctccg
     5461 ccggtttttc tccatcgcct cgttcatact ctgttcgtgg gcgctgatgg cgtcgctgat
     5521 tctcctcgcg cttgcgctca gttccgctct gacagactgc tcgtgctcgc tgaatgcggc
     5581 tttcagcatc tcgccggtac tcttctgctg cgctttcgat tgctgctcta agtccttcgc
     5641 cagcgttaaa agactgctca tagatggctc ccttcagtcg gatgtttcgc cccccgtcag
     5701 ggtcggcaat gctgatactg cttttcgtgg tgcgtaccac ctcaaaacct gaccgctcaa
     5761 gcgcctcagt gacgtcatga cggcttttca gctccccgga cgaggccaag ctcagcagcc
     5821 ccctggtgat ggcttctgcg gcatcctgct tcgttttcgg cagcccggcc ggcgtgacca
     5881 gcgcccgacg gttttccggc gcgttcgggt cgagcagccc cagccggccg ttgaccaccg
     5941 tctgccaggc atcgatgcgt ggccggtcgg cgcggtcata gtacggctgg agccgcctgc
     6001 cggtcagcag ctcggtattg gggatcagga agttcagctc cagacgcccc ttgtccgcgt
     6061 gctccaccca cagaatgctg tactgatctt tatcgagtcc gggcatcaga acccgctcga
     6121 agctggccat cagtttttcc cgctggccgg gaggcagatc ggcctccgca aacgacagta
     6181 cgccggaggt gtatttcttc acgtaaggcg acgcatcaat cagctcccgg acttcttccg
     6241 gcttgccctg caggacggtg gcgccttcac gctgccggtc tttacccagc agatagtcca
     6301 ccggcccggc gccaccgccg cggccgcgcg gatgaaattt aacgatcatc gtccattcct
     6361 ttct
//
