LOCUS       NZ_CP053475             2509 bp    DNA     circular CON 17-JUL-2023
DEFINITION  Staphylococcus warneri strain WB224 plasmid pWB224_5, complete
            sequence.
ACCESSION   NZ_CP053475
VERSION     NZ_CP053475.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10970963
            Assembly: GCF_013137955.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus warneri
  ORGANISM  Staphylococcus warneri
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Li,Z., Dong,L., Zhao,C. and Wang,X.
  TITLE     Complete genome sequence of Staphylococcus warneri WB224, a strain
            with high-salted tolerant and acid-producing properties
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Li,Z., Dong,L., Zhao,C. and Wang,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-MAY-2020) Traditional Food Research, Institute of
            Agro-Products Processing Science and Technology of Saas, Jingjusi
            Road 20#, Chengdu, Sichuan 610066, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 22-JUL-2019
            Assembly Method                   :: Unicycler v. v0.4.8-beta
            Assembly Name                     :: WB224_Unicycler_v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 500.0x
            Sequencing Technology             :: PacBio Sequel; Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/17/2023 03:32:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,428
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 13 of 39
            Pseudo Genes (incomplete)         :: 20 of 39
            Pseudo Genes (internal stop)      :: 11 of 39
            Pseudo Genes (multiple problems)  :: 4 of 39
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP053475.1.
            Bacteria and source DNA available from Zhihua Li.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Staphylococcus warneri"
                     /mol_type="genomic DNA"
                     /strain="WB224"
                     /isolation_source="Doubanjiang"
                     /db_xref="taxon:1292"
                     /plasmid="pWB224_5"
                     /geo_loc_name="China:Chengdu"
                     /collection_date="2016-08-25"
     gene            join(1753..2509,1..167)
                     /locus_tag="EYQ96_RS00460"
                     /old_locus_tag="EYQ96_00465"
     CDS             join(1753..2509,1..167)
                     /locus_tag="EYQ96_RS00460"
                     /old_locus_tag="EYQ96_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002499696.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_145414927.1"
                     /translation="MKKYTEKKQRNQVFQNFIKRHIGENQMDLVENCNTFLSFVTNRRM
                     DKKKLYKSNPCKNRFCPMCAWRKARKDALGLSLMMQHIKQAEDKQFIFLTLTTPNVTSE
                     HLESEIKHYNQSFRRLSNRKHFKSIAKGYVRKLEITYNKKRDDYNPHFHVLIAVNKSYF
                     KDTKAYITQKEWLEMWRDVTGNSEITQVHIQKVKQNNNKELYEMAKYSGKDNDYLINQK
                     VFDAFYKALKGKQVLVYSGLFKDARKKLKNGELDYLKEVDPTEYIYQIFYTWNKKEYLA
                     SEIFDLTESEKQEINHQMIDEIEEEK"
                     /name="protein rep"
     gene            complement(1015..1500)
                     /gene="lnu(A)"
                     /locus_tag="EYQ96_RS00465"
                     /old_locus_tag="EYQ96_00470"
     ARG             complement(1019..1500)
                     /name="lnu(A)"
                     /gene="lnu(A)"
                     /gene_name="lincosamide nucleotidyltransferase Lnu(A)"
                     /tool="amrfinderplus"
                     /drug_class="LINCOSAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(1016..1500)
                     /name="lnuA"
                     /gene="lnuA"
                     /gene_name="lincosamide nucleotidyltransferase (LNU)"
                     /tool="rgi"
                     /drug_class="lincosamide antibiotic"
                     /identity="97.52"
                     /coverage="100.0"
     Typing          1754..2509
                     /name="rep_cluster_2133"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.62"
                     /coverage="82.0"
                     /evalue="0.0"
ORIGIN
        1 gagaattaga ttacttaaaa gaagttgatc cgaccgaata tatttatcaa attttttata
       61 cttggaataa aaaagagtat ttagcgagtg aaatttttga tttaacagaa agtgaaaaac
      121 aagaaattaa tcatcaaatg attgatgaaa ttgaagaaga aaaataaaaa taaattttct
      181 taaacgttag caaaaagcta gcgttttttt tgttgaaaat acgttaaaaa tggctgggta
      241 caaactgggt acagacacaa tatatagggg tacaaccggg gtacaatcgc aatttgtagg
      301 ggtacaatcg gggtacaaaa taaatttcta ggcacctatg aaaaatctct gtatcccttg
      361 aaatgtctgg cttcgccaga cccacctttt taaaaggtgg caaatttccc ttatgctctt
      421 aactcttgat ttgggttcaa ttcaaaattt ggtatttttt gaaagaaatt gcccctgcag
      481 gaactaaaaa agttgatgaa tttttgagcc attttatgcc aatttttaat ttgcatataa
      541 ctgggcagtg tctaaaaaaa cggcactggt tttgctcaaa tttttgtttg tgaaattaaa
      601 atatatttat ttggctctca tttgcgtttt aagagccaat ataagttttt gggtataaaa
      661 ctatataaat aactctaatt tttcaaaata ccgccttaaa attaaaaata aaggtattct
      721 gatttgtaat aaattaaaat ataaaaaaag agccaccatt taaaatttag gcagctctta
      781 ctcacttttt ggatgataaa agtggaaaat taattcgaga aaaaacagaa aggatatttt
      841 aattataaca cggcatttat gccgagaaaa tttattgatg ttgagaagaa cccttaacta
      901 aacttgtaga cgaatgtcgg tatagcgtga gctattaaac cgaccattcg acaagttttg
      961 ggattgttaa gggttccgag gctcaacgtc aataaagcaa ttggaataaa gcaattatgt
     1021 tattgatttt aaatttttta tatcaaaatg gtctttttct gttaaatcat aaccagaatg
     1081 aaaaagaagt tgagcttctt ttgaaataca tggtattttt cgacctttgt aattagtttc
     1141 tgaaaaccag tcattttgga aaacataatt accaccttct gggtttgctt gggtaattga
     1201 tccatcatca tttagattta taggatgaat atctaaatac ccatattctt catgcttaag
     1261 ttccatacgt gaaggcatcc aatcaacttc tattttgtat cctatatctt ctaatttttg
     1321 tataactttt tgagtgtgtt gagcgtcaaa atctatatct atatctctgt gttctctttg
     1381 ttgttttcca gttaatacat ctacccccca gccaccatct aaccaataag ttactttcat
     1441 gtgttcaaat aaatctaaaa tataaaataa atctttttct gttacattat tatttttcat
     1501 tttatccctc ctaacttggg aatttgaaac aagttgaaaa cgagtttctt cttgtcttga
     1561 taataagggt aactattaca agctgtgata gttaccctta ttccattgat atgactggtt
     1621 ttaagcacaa aaaaagttgc cttttccagc atactattgt atcgttttag gtgtctaagc
     1681 taaacgaaaa atagaaagga gaaaaagcaa cgtcttttcg tatttcaaac aaagttgttt
     1741 tatgtgaatt atatgaaaaa gtatactgag aaaaaacaaa gaaatcaagt atttcaaaac
     1801 tttattaaac gacatattgg ggaaaatcaa atggatttag ttgagaattg taatacattt
     1861 ttgtcttttg tgacgaatag aagaatggac aaaaagaaat tatataaatc taatccatgt
     1921 aaaaatcgtt tttgtccgat gtgtgcatgg cgaaaggcga gaaaagatgc attaggtttg
     1981 tcattgatga tgcagcatat taagcaagca gaagataagc aattcatctt tttaacactt
     2041 acaacgccaa atgtaacaag tgaacatttg gaaagtgaaa taaaacatta taatcaatca
     2101 ttcagacgat taagtaatcg taaacatttt aagtctatag ctaaaggtta tgttagaaaa
     2161 ttagaaatta cttataacaa aaagcgtgat gattataacc cacattttca tgtattgatt
     2221 gccgttaata aatcatattt taaagatact aaagcttata ttactcaaaa agaatggcta
     2281 gaaatgtggc gtgatgtcac aggaaattct gaaattacac aagtacatat acaaaaagtt
     2341 aagcaaaata ataataaaga actttacgaa atggctaaat attctggtaa ggataatgac
     2401 tatttaatta atcaaaaagt gttcgacgca ttttataaag cgttaaaagg aaaacaagtt
     2461 cttgtttatt ctggactgtt taaagacgca agaaagaaat taaaaaatg
//
