LOCUS       NZ_CP053474             3732 bp    DNA     circular CON 17-JUL-2023
DEFINITION  Staphylococcus warneri strain WB224 plasmid pWB224_4, complete
            sequence.
ACCESSION   NZ_CP053474
VERSION     NZ_CP053474.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10970963
            Assembly: GCF_013137955.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus warneri
  ORGANISM  Staphylococcus warneri
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Li,Z., Dong,L., Zhao,C. and Wang,X.
  TITLE     Complete genome sequence of Staphylococcus warneri WB224, a strain
            with high-salted tolerant and acid-producing properties
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3732)
  AUTHORS   Li,Z., Dong,L., Zhao,C. and Wang,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-MAY-2020) Traditional Food Research, Institute of
            Agro-Products Processing Science and Technology of Saas, Jingjusi
            Road 20#, Chengdu, Sichuan 610066, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 22-JUL-2019
            Assembly Method                   :: Unicycler v. v0.4.8-beta
            Assembly Name                     :: WB224_Unicycler_v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 500.0x
            Sequencing Technology             :: PacBio Sequel; Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/17/2023 03:32:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,428
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 13 of 39
            Pseudo Genes (incomplete)         :: 20 of 39
            Pseudo Genes (internal stop)      :: 11 of 39
            Pseudo Genes (multiple problems)  :: 4 of 39
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP053474.1.
            Bacteria and source DNA available from Zhihua Li.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3732
                     /organism="Staphylococcus warneri"
                     /mol_type="genomic DNA"
                     /strain="WB224"
                     /isolation_source="Doubanjiang"
                     /db_xref="taxon:1292"
                     /plasmid="pWB224_4"
                     /geo_loc_name="China:Chengdu"
                     /collection_date="2016-08-25"
     gene            join(3510..3732,1..425)
                     /locus_tag="EYQ96_RS00445"
                     /old_locus_tag="EYQ96_00450"
     CDS             join(3510..3732,1..425)
                     /locus_tag="EYQ96_RS00445"
                     /old_locus_tag="EYQ96_00450"
                     /EC_number="2.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-8 chloramphenicol O-acetyltransferase"
                     /protein_id="WP_002352254.1"
                     /translation="MTFNIINLETWDRKEYFNHYFNQQTTYSVTKEFDITLLKSMIKNK
                     GYELYPALIYTIVNIINQNKVFRTGINSEGNLGYWDKLNPLYTVFNKETEKFSNIWTES
                     NVSFNSFYNSYKSDLLEYKDKNEMFPKKPIPENTVPISMIPWIDFSSFNLNIGNNSRFL
                     LPIITIGKFYSKNNKIYLPVSLQVHHAVCDGYHVSLFMSEFQNIVDSVNEWI"
                     /name="type A-8 chloramphenicol O-acetyltransferase"
     gene            690..1907
                     /gene="mobV"
                     /locus_tag="EYQ96_RS00450"
                     /old_locus_tag="EYQ96_00455"
     CDS             690..1907
                     /gene="mobV"
                     /locus_tag="EYQ96_RS00450"
                     /old_locus_tag="EYQ96_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002493196.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_002493196.1"
                     /translation="MSYSIIRVSKVKSGTNTTGIQKHVQRENNNYENEDIDHSKTYLNY
                     DLVNEYNQNFNNLIDEKIEQNYTGKRKIRTDAVKHVDGLITSDKEFFDNQTPEDTKQFF
                     EHAKEFLEQEYGKDNLLYATVHMDEKTPHMHYGVVPITDDGRLSAKQVVGNKKALTEFQ
                     DRFNEYVNQRGYDLERGQSRQVTNAKHEQMSQYKQKTEYYKQEYERESQKTDEIKQKND
                     KLVREYQKSLNALKKPINVPYEKETKTVGGLFNKETQETGNVVIRQEDFNAFQKQIEAA
                     QSITDDYEYIKSDKALNDLKSENNELKQENENLRNDKSELRRHFKKEHAQNKHLKDLIN
                     YLRRLLEKLEKTLGRKNYIDLINGMTENNDKDRKIALTFDKEMHPEKYQEQEQQEEVKK
                     DRGRGR"
                     /name="mobV"
     gene            2431..3375
                     /locus_tag="EYQ96_RS00455"
                     /old_locus_tag="EYQ96_00460"
     CDS             2431..3375
                     /locus_tag="EYQ96_RS00455"
                     /old_locus_tag="EYQ96_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017722130.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_023607346.1"
                     /translation="MSKKAEEIQSKQSSEHENLNFSKTGYSNSRLNRHTMYTPEPKLSF
                     DAMTIVGNLNKNNAHKLSEFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADTWD
                     RRNMRVEFNPNKLTHEEMLWLKQNIIDYMEDDGFTRLDLAFDFEYDLSDYYAMTDKSVK
                     KTIFYGRNGKPETKYFGVRDSDRFIRIYNKKQERKDNADIKIMSEHLWRVEIELKRDMV
                     DYWNDCFNDLHILQPDWKTIERTSDRAMVFMLLNDEEEWGKLERRTKNKYKKLIKEISL
                     IDLTDLMKSTLKANEKQLQKQIDFWQREFRFWK"
                     /name="replication initiation factor domain-containing
                     protein"
     ARG             4..425
                     /name="catA8"
                     /gene="catA8"
                     /gene_name="chloramphenicol acetyltransferase (CAT)"
                     /tool="rgi"
                     /drug_class="phenicol antibiotic"
                     /identity="99.29"
                     /coverage="65.12"
     ARG             3511..3731
                     /name="catA8"
                     /gene="catA8"
                     /gene_name="chloramphenicol acetyltransferase (CAT)"
                     /tool="rgi"
                     /drug_class="phenicol antibiotic"
                     /identity="100.0"
                     /coverage="34.42"
     Typing          2432..3375
                     /name="rep_cluster_1118"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.259"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          691..1184
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.303"
                     /coverage="92.0"
                     /evalue="3.71e-98"
ORIGIN
        1 gaaatttggg ttattgggat aaattaaacc ctttatatac agtctttaat aaagaaactg
       61 aaaaattttc taacatttgg acagaatcaa atgttagttt taattctttt tataatagtt
      121 ataagagtga cttacttgaa tataaagata aaaatgaaat gtttcctaaa aaaccaatac
      181 ctgaaaacac agttcctatt tcgatgattc cttggattga ttttagttca tttaatttaa
      241 atattggtaa taatagtaga ttcctattgc caattattac aataggtaaa ttttatagta
      301 agaataataa gatctattta ccagtctcat tgcaagttca tcatgcggta tgtgatggtt
      361 accatgtttc attatttatg agtgaatttc aaaatatagt tgatagtgta aatgaatgga
      421 tttaaatcta aaataacttt cataaactta agtaattatg atatttataa gtttatagca
      481 accaccattt ttggttgctg gattttgatt ttgaatttgg ttttgaactt atggactgac
      541 ttgttcagtc cattttttgt gcttgcacaa aaaatggttt tagcctcgca gagcatacgg
      601 tttaacgact tattgcaaaa taagtctagt atgttagact taaactatta aatacacatg
      661 aaacctttgt gcttaggagt gatttttata tgtcttattc cattattaga gtttcaaaag
      721 taaagtctgg aacaaataca actggtattc aaaaacatgt tcaaagagaa aataataatt
      781 atgaaaatga agatatagac catagtaaaa cttatttgaa ttatgatttg gtaaacgaat
      841 ataaccaaaa ttttaataac ttgattgatg aaaaaataga acaaaattat acaggtaaaa
      901 gaaaaatcag aacggatgca gttaaacacg ttgacggttt aattacatca gacaaagaat
      961 tttttgataa tcaaacacca gaagatacaa agcagttttt tgaacatgct aaagaatttt
     1021 tagaacaaga atacggtaaa gataatttat tatatgcaac agttcatatg gacgagaaaa
     1081 caccacatat gcattatggt gtagttccaa taactgatga tggtcgatta agtgctaaac
     1141 aagttgtagg gaataaaaag gctttaacag agtttcaaga taggtttaac gaatatgtta
     1201 atcagcgtgg ctatgattta gaacgtgggc aatccagaca agttacaaat gcaaagcatg
     1261 agcaaatgag tcagtataaa cagaaaacag aatattataa gcaagaatat gagcgtgaga
     1321 gccaaaaaac agacgaaata aagcaaaaga acgataaatt ggtgcgagag taccaaaagt
     1381 cattaaatgc tcttaaaaag cctataaatg tcccgtatga gaaagaaact aaaacagtag
     1441 gtggtttatt taacaaagaa acacaagaaa ctggaaatgt tgtaataaga caagaagatt
     1501 ttaatgcctt tcagaagcaa atagaagcag cacaatcaat tacagatgat tatgagtata
     1561 ttaagtcaga taaagcttta aatgacctta aaagtgaaaa taatgagctt aaacaagaaa
     1621 atgaaaatct tagaaacgat aaaagtgaat taagaaggca ttttaaaaaa gagcatgctc
     1681 aaaataaaca cctaaaagac cttataaatt atttaagaag attattagag aagttagaaa
     1741 aaactttagg tagaaaaaat tatattgatt tgattaacgg tatgactgaa aacaatgata
     1801 aagatagaaa aatagcatta acgtttgata aagaaatgca cccagaaaaa tatcaagagc
     1861 aggaacaaca agaagaagtt aaaaaagata gaggacgtgg aagataaaaa actactgtta
     1921 ataagcgccc taaacgttat aacagcagtt ttaaacctca ttcaaagcct atttaactaa
     1981 atacaatctt gtattggtcg tactatcgca tattataaca cgctattatg ccgagaaaac
     2041 ttattgtttg gaatgggcta tgtgttagct aacttgatta gcgtgttggg tggggttgat
     2101 ttgggattaa tcccaatgaa acaccaacac aacaacacat aaagccctgt aggttccaaa
     2161 caataaggaa attggaataa tgcaataaac taaaaaagcc ttgatctaaa tagaccaaga
     2221 cttttgatag tgttatatta ataacaaaat aaaaataaag aagtcgtcca ctccccgacc
     2281 aaagtttgtg gaacgaccat accaaatata ctgattatgt tatttgtaat ttttgtataa
     2341 ttagatatta aacgatattt gattattctt caacatttac atttgacgat tggatggacg
     2401 acttcttaaa aattaaggag tcgatttttt atgagtaaaa aagcagaaga aattcagtca
     2461 aaacaaagtt cagaacacga aaatttaaat ttttctaaaa ccggctactc taatagccgg
     2521 ttaaaccgac atactatgta caccccggaa ccaaaattaa gttttgacgc tatgactatt
     2581 gttggaaatc ttaataaaaa taatgctcac aaactatctg aatttatgag tgtcgagcca
     2641 caaattcgac tttgggatat actacaaact aaatttaaag ctaaagctct acaagaaaaa
     2701 gtttatatcg aatatgacaa agtaaaagca gatacgtggg atagacgtaa tatgcgtgtt
     2761 gaatttaatc caaataaact tacgcatgaa gaaatgcttt ggttaaaaca aaacattatc
     2821 gactacatgg aagacgatgg ttttacaaga ttagatttag cttttgattt tgaatatgat
     2881 ttaagtgatt attatgcaat gactgataaa tcagttaaga aaactatttt ttatggtcgt
     2941 aacggtaaac cagaaacgaa atattttggt gttcgtgaca gtgatagatt tattagaatt
     3001 tataataaaa aacaggaacg caaagataat gcagatatta aaattatgtc tgaacactta
     3061 tggcgtgtag aaattgaatt aaaaagagat atggttgatt attggaacga ttgttttaat
     3121 gatttacata tattacaacc agattggaaa actatcgaac gtacttctga tagagcaatg
     3181 gtttttatgt tgttgaatga tgaagaagaa tggggaaaat tagaaagacg tactaagaat
     3241 aaatataaaa aattaattaa agaaatatct ctaattgatt taactgattt aatgaaatcg
     3301 actttaaaag cgaacgaaaa acaattgcaa aagcagattg atttttggca acgtgaattt
     3361 agattttgga agtaaagtaa ctttgtttta tacaaaatgt tactttagta taattaacgt
     3421 ttaagaggtg acacaatata tgaaaaaatc agaggattat tcctcctaaa tacaaagaga
     3481 taagatgaaa tttaggagga aaattatata tgacttttaa tattattaat ttggaaactt
     3541 gggatagaaa agaatatttt aatcattatt tcaatcaaca aacaacttac agtgttacta
     3601 aagaatttga tatcacttta cttaaaagta tgataaaaaa taaaggatat gaactgtatc
     3661 ctgctttgat ttatacaatt gtaaatatta taaatcaaaa taaagtattt agaacaggaa
     3721 ttaatagtga gg
//
