LOCUS       NZ_CP053473             4065 bp    DNA     circular CON 17-JUL-2023
DEFINITION  Staphylococcus warneri strain WB224 plasmid pWB224_3, complete
            sequence.
ACCESSION   NZ_CP053473
VERSION     NZ_CP053473.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10970963
            Assembly: GCF_013137955.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus warneri
  ORGANISM  Staphylococcus warneri
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4065)
  AUTHORS   Li,Z., Dong,L., Zhao,C. and Wang,X.
  TITLE     Complete genome sequence of Staphylococcus warneri WB224, a strain
            with high-salted tolerant and acid-producing properties
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4065)
  AUTHORS   Li,Z., Dong,L., Zhao,C. and Wang,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-MAY-2020) Traditional Food Research, Institute of
            Agro-Products Processing Science and Technology of Saas, Jingjusi
            Road 20#, Chengdu, Sichuan 610066, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 22-JUL-2019
            Assembly Method                   :: Unicycler v. v0.4.8-beta
            Assembly Name                     :: WB224_Unicycler_v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 500.0x
            Sequencing Technology             :: PacBio Sequel; Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/17/2023 03:32:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,428
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 13 of 39
            Pseudo Genes (incomplete)         :: 20 of 39
            Pseudo Genes (internal stop)      :: 11 of 39
            Pseudo Genes (multiple problems)  :: 4 of 39
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP053473.1.
            Bacteria and source DNA available from Zhihua Li.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4065
                     /organism="Staphylococcus warneri"
                     /mol_type="genomic DNA"
                     /strain="WB224"
                     /isolation_source="Doubanjiang"
                     /db_xref="taxon:1292"
                     /plasmid="pWB224_3"
                     /geo_loc_name="China:Chengdu"
                     /collection_date="2016-08-25"
     gene            complement(join(3549..4065,1..413))
                     /locus_tag="EYQ96_RS00420"
                     /old_locus_tag="EYQ96_00425"
     CDS             complement(join(3549..4065,1..413))
                     /locus_tag="EYQ96_RS00420"
                     /old_locus_tag="EYQ96_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002489532.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_171484822.1"
                     /translation="MTEENHSNYLQNRDLDNFSKTGYSNSRLSGNFFTTPQPNLSFDAM
                     TIVGNLNKDNAEQLSKFMSVEPRFRLWDILQTKFKAKALQEKVYIEYDKVRADSWDRRN
                     MRVEFNPNKLTYDEMLWLKRNVISYMEDDGFTRLDLAFDFEEDLSDYYTMTDKAVKKTI
                     FYGRNGKPETKYFGVRDSDRFIRIYNKKQERKDNADIEINSEHLWRVEIELKRNMVDMW
                     NSCFDDLHILKPNWTVLEKIKEQAMVYMLIHEEDTWGKLERHAKYKYKNLIKEISPVDL
                     TELMKSTLRENEKQLQKQIDFWLSDFEF"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            complement(929..1231)
                     /locus_tag="EYQ96_RS00425"
                     /old_locus_tag="EYQ96_00430"
     CDS             complement(929..1231)
                     /locus_tag="EYQ96_RS00425"
                     /old_locus_tag="EYQ96_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019236263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015365428.1"
                     /translation="MFKKFFTISVVLFAIDMLSVVFKDFFLLGEKTTTMNGFLLFLTIL
                     FTISMIVTTFFYFISKKNHNTEEYKGLYFTVLISLPFVWLIVGHIITSPLPYILK"
                     /name="hypothetical protein"
     gene            complement(1487..2182)
                     /locus_tag="EYQ96_RS00430"
                     /old_locus_tag="EYQ96_00435"
     CDS             complement(1487..2182)
                     /locus_tag="EYQ96_RS00430"
                     /old_locus_tag="EYQ96_00435"
                     /inference="COORDINATES: protein motif:HMM:NF015839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF334 domain-containing protein"
                     /protein_id="WP_077126318.1"
                     /translation="MSLKDEREKKKNQSETQSQSTQTNSKSIGTQSNTTQSNLERQEIE
                     ELKKQNKLIIKYLTEIQENQNAREKDQKEITSKLTGATKDFRDNGVKTHNAFINILKEK
                     LDKVDTDELANIIGRDIYKVRDENERMLQEVRSSHEEYQKRIKLMYRGFGAMLLVFMLF
                     ALVMTIGSDFMDFIHIDILQKAIASKIKASEGFMTFIWYIAYGLPYIVAIGGFIYLYEW
                     IRKKFEVFF"
                     /name="DUF334 domain-containing protein"
     gene            complement(<2226..2879)
                     /locus_tag="EYQ96_RS00435"
                     /old_locus_tag="EYQ96_00440"
                     /pseudo=""
     CDS             complement(<2226..2879)
                     /locus_tag="EYQ96_RS00435"
                     /old_locus_tag="EYQ96_00440"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2861..3244)
                     /locus_tag="EYQ96_RS00440"
                     /old_locus_tag="EYQ96_00445"
     CDS             complement(2861..3244)
                     /locus_tag="EYQ96_RS00440"
                     /old_locus_tag="EYQ96_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002449121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_019236260.1"
                     /translation="MSEQNNFVASDETVGRNHKPNRKEPKQISFRVSESEYLKLKQSAE
                     TLNMSVPNFVKKKAQGSRLVAPKFDKATRQSIAKDLSKLGANANQIAKYCNQHQYDAPN
                     YPALERNISELRERLDEIWQQLN"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          3216..3452
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="81.667"
                     /coverage="100.0"
                     /evalue="8.37e-48"
     Typing          complement(2..413)
                     /name="rep_cluster_1257"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(3570..4065)
                     /name="rep_cluster_1118"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.569"
                     /coverage="80.0"
                     /evalue="2.69e-136"
     Typing          complement(1921..2879)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.625"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aatcttgtaa atccatcatc ttccatgtag ctaattacat tgcgttttaa ccaaagcatt
       61 tcatcatacg ttaatttatt aggattaaat tcaacacgca tattacgtct atcccaacta
      121 tctgctctca ctttgtcata ttcgatatat actttttctt gaagtgcttt agctttgaat
      181 ttggtttgta gaatatccca aagtctaaat cgaggctcaa cactcataaa ttttgataat
      241 tgttctgcat tatctttatt aagatttcca acaattgtca tagcgtcaaa acttaaattt
      301 ggttgagggg tggtaaaaaa attaccactt aaccggctat tagagtagcc ggttttagaa
      361 aaattgtcta aatccctatt ttgtaagtaa tttgaatgat tttcttcagt cataaaaaat
      421 cgactcctta atttgtttta agaagtcgtc cacccaaacg tcaaatataa atgttgaagt
      481 ataatcaaat atcgtttaat atcaaaatat acaaaaacat aaaaacatcg actgttttat
      541 attttggcta gtcgttcacg taaactttgg tcggtttggg gtggacgact tctttttttt
      601 attttgttat taatataaca ctaacaaaaa gcttggtcta ttatggctaa gctttctgtg
      661 tatcaaataa aaaaagattc aatcacagaa aatatagatg tgattgaatc ttttttagtt
      721 cctgcttatt tagcagtaca attaacccca tcattattat tagggcaact atgttttaaa
      781 tgaagcttgt tacctagttt tattcctatt ccccaaggat taggtaaatt catactaaca
      841 acctccttta aatatagtga taatttacac ttatatcttt acatatttct ataaattttg
      901 caattaaaag caaaatagtt atatatatct attttaatat atatggaaga ggcgaagtta
      961 taatatgtcc aactattaac catacaaacg gaagagaaat taaaacagta aaatataatc
     1021 ctttatactc ttcagtatta tgattttttt tactaataaa ataaaaaaat gttgttacaa
     1081 tcatagagat agtaaacagt attgttaaaa acaataaaaa tccattcatt gtcgttgtct
     1141 tttctccaag taaaaagaaa tctttaaata caaccgaaag catatcaatt gcaaataaaa
     1201 caacactaat tgtaaaaaac ttcttaaaca taaattacac ctcctatacc tgatttgttt
     1261 atttaaaaat tgtttagagc cattaataac ataatttacc ttatctttat attttattgc
     1321 attattccaa ttcctctatt tgtcgacgat tctgcgctcc ttaataatcc taaaactgtc
     1381 catcggtcga tttgatagct gacgctatcc gaccgctggc gcttttagtc taattaaggg
     1441 ctctcatcga caaacagatt ttctcggcat aatagcgtgc ttatccttag aaaaagactt
     1501 cgaatttttt tctaatccat tcataaagat aaataaaacc acctatcgca acaatataag
     1561 gtaatccata agcaatatac caaataaatg tcataaagcc ctcagacgct ttaattttgc
     1621 ttgctattgc tttttgtaat atatctatgt gtataaagtc cataaagtca ctgccaatag
     1681 tcattactaa cgcaaatagc ataaatacta ataacattgc tccaaatcca cgatacatga
     1741 gtttaattct tttttgatac tcttcatgtg atgatcgcac ttcttgtagc attcgttcgt
     1801 tttcatcacg caccttataa atatctctgc ctataatatt ggctaactca tctgtatcga
     1861 ctttatctaa tttctctttg agtatattta taaaagcatt atgtgtcttt acgccattat
     1921 ctcgaaaatc cttcgtcgcc cctgtcaatt tcgatgtgat ttccttttgg tctttctctc
     1981 gtgcgttctg attctcttga atctctgtta gatacttgat gatgagcttg ttttgttttt
     2041 tcaattcttc gatttcttgt ctttctaagt tgctctgcgt tgtgttcgac tgcgtcccaa
     2101 tcgacttcga attggtctgt gtactctgag actgggtctc gctctgattc tttttcttct
     2161 ctctctcgtc tttcaaactc atattctaac gcccccttat cgtaatcttg tccaagggtt
     2221 ttgccacgta cccatttatt tacgttttcc ggcttatatg agacgttttt gccccttacc
     2281 ttaaattcta tacctgattt ctctaaatgc tctgaaaaac tcttaaaatc gcttgtatgt
     2341 tctttcgcat gttctatttt ttctctcaaa tcatctttcc aagaatattg gtctttttct
     2401 atcaatgatt tttcagcttg tgtgtatctc aatttcgccg tatctcgttc tggcacactc
     2461 aaaccatgtt ctcgacaaat atcatcattg gcttgtttaa caaaatctct ttgttcttta
     2521 ttactttgat atttgcgacc tgtctcaagg tcaattgaat taattacaat atggttgtgt
     2581 acatgttctt tatctgtatg cgtataaact gctacttgat gattaggtgc aatcttctcg
     2641 actaattcca aaccaagttg gttacattgt tctggtgtta cttcaccggg cttgaaagac
     2701 tgtataatcg tatgcgcttg aatcccatct tctttaccat acaaggctct tgtttgttta
     2761 aaagccgatt tcgcataatc tacatcacaa ttcaagccac ttttttcagt tgctcgcttt
     2821 tctgcgtaat tgattgctcg acttgccgat ttggtattgc ctaatttagt tgttgccata
     2881 tctcatcaag cctttcacgt aactcactga tattacgttc taacgctgga tagttaggtg
     2941 cgtcatattg atgttgatta caatatttcg caatctgatt cgcatttgca cctaacttac
     3001 ttaaatcttt ggctatcgat tgtcgcgttg ctttatcaaa tttaggtgcg actaatcgac
     3061 taccctgtgc ctttttctta acgaaattag gcacactcat atttaaagtt tcagcagact
     3121 gctttaactt taaatattca gattcgctca cacgaaaact aatttgtttc ggctccttac
     3181 ggttgggttt atggtttcgc ccaacagttt catcgctagc cacaaagtta ttttgttcgc
     3241 tcatcaaatc ccaccacgct ttcttcgaaa gtcattactt aatataaatc tagcttacag
     3301 ggtaagccag attttatcaa gtaattttgt ggtttggcaa gcgtcgtatt ttctagccac
     3361 ttcgttctta aaataccggc ttgctgggat taatcccaga ccctttatgt ttttgcttac
     3421 gcaaaaaatg agtggcttaa atgctagcca ctcatagttc aaaaccataa atcccaagcg
     3481 accaaaaatg gtggtcgcat attcgttatt aaattttatg atcacttcgt gtataaaatt
     3541 taatacactc aaaattcaaa atcagaaagc caaaaatcaa tctgcttttg taattgtttt
     3601 tcattttctc ttaaagtcga tttcattaat tcagttaaat caactggtga tatttcttta
     3661 attaagtttt tatatttata tttagcatgt cgttcaagtt ttccccatgt atcttcttca
     3721 tgaattaaca tataaaccat tgcttgctct tttatttttt ctagtacagt ccaattaggt
     3781 tttaatatat gcaaatcatc aaaacagcta ttccacatat caaccatatt tcttttaagc
     3841 tcaatttcta cacgccataa atgttctgaa ttaatttcaa tatcggcatt atctttgcgt
     3901 tcttgctttt tattataaat tcgaatgaat ctatcactat cacgtacacc aaaatatttt
     3961 gtttctggtt taccattacg cccataaaat atagttttct taactgcttt atcagtcatt
     4021 gtgtaataat cgcttaaatc ttcttcaaaa tcgaaagcca aatct
//
