LOCUS       NZ_CP053387             2074 bp    DNA     circular CON 02-JUL-2023
DEFINITION  Escherichia coli strain SCU-107 plasmid pSCU-107-3, complete
            sequence.
ACCESSION   NZ_CP053387
VERSION     NZ_CP053387.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14609772
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Stephens,C., Arismendi,T., Wright,M., Gonzalez,A., Gill,M.,
            Hartman,A., Pandori,M. and Hess,D.
  TITLE     F plasmids are the major carriers of antibiotic resistance genes in
            human-associated commensal E. coli
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Stephens,C., Arismendi,T., Wright,M., Gonzalez,A., Gill,M.,
            Hartman,A., Pandori,M. and Hess,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAY-2020) Biology, Santa Clara University, 500 El
            Camino Real, Santa Clara, CA 95054, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method              :: Unicycler v. 0.4.8
            Genome Representation        :: Full
            Expected Final Version       :: Yes
            Genome Coverage              :: 54x
            Sequencing Technology :: Illumina MiSeq, Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 07/02/2023 04:31:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 2
            CDSs (total)                 :: 2
            Genes (coding)               :: 2
            CDSs (with protein)          :: 2
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP053387.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="SCU-107"
                     /isolation_source="rectal swab from healthy college
                     student"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pSCU-107-3"
                     /geo_loc_name="USA: California"
                     /collection_date="2015"
     gene            complement(join(1774..2074,1..662))
                     /locus_tag="HHJ25_RS00005"
                     /old_locus_tag="HHJ25_23885"
     CDS             complement(join(1774..2074,1..662))
                     /locus_tag="HHJ25_RS00005"
                     /old_locus_tag="HHJ25_23885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_128430403.1"
                     /translation="MSEDKFLSDYSPRDASWDTQRAFTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTLMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVAD
                     YPSSRWLFLTLTVRNCAIDDLGETLTAMNSAFQRLKDRKEFAPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVPPSWFTRDYVKQARWVELWRDCLRVNYEPNIDIRAVKTKTGEVVANV
                     AEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETNE
                     DLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     gene            complement(1504..1770)
                     /locus_tag="HHJ25_RS00010"
                     /old_locus_tag="HHJ25_23890"
     CDS             complement(1504..1770)
                     /locus_tag="HHJ25_RS00010"
                     /old_locus_tag="HHJ25_23890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
ORIGIN
        1 ttggccacaa cctcacctgt cttagttttt actgcccgaa tatcgatatt cggctcatag
       61 ttcacccgca agcaatcgcg ccagagttcc acccaacgag cctgttttac ataatcacgt
      121 gtgaaccatg acggcggaac catcagtaaa cagtgaaaat gcggatgtgc actgccatct
      181 tttccccgtg taacttccgt cgcgcgaatc cagccctgaa ccggcgcgaa ctctttcctg
      241 tccttcagtc gctgaaaggc cgaattcatg gctgtcagcg tttcccccaa atcatcgatc
      301 gcgcagttcc tgacggttaa cgtcagaaac aaccaacggg aagatgggta atccgcaacg
      361 atttttggta gagcctgata aaaacgagct tgccacatga gggttcttct ccactggcag
      421 acaggacaat gacggacacg gcaaaattgc gcgctacgaa gccgtaggcg cgtttctccg
      481 gtttccatga gcgtagacca gccaaaacgc aataaaccgc tacaggaggc catacggagc
      541 gcgtagcgct cgaattccgc agcggtctga tagataccgc ccacggaatc ggtaaacgcg
      601 cgctgggtat cccaagacgc atcacggggg ctgtagtccg aaaggaattt atcttctgac
      661 attcagcctg ctccgatctg ggcaggctgt gatttgatgt ggtatattca tcacgtacct
      721 gtgcattgcg tgccagtaca tgtcaccaga gtttgccgct gaggtgacac attcgaaatg
      781 ttggggagtg ttgacgcact ccccaacgcc gattaaaaac acctcaattc tgcgccaaaa
      841 tcttctacac gtcaaacacc caccaaagcc gcgccattgc tggcctgaac ctatattttc
      901 catcccagag ttatccctta agtataaata caaggatcac gcgcgagaat tgcgcaaaaa
      961 ataaccacaa ttttaacccc ccaaaacaag cacccccgaa cggcctaccc caaccttttc
     1021 cacacacccg atcaccaaaa caggggataa aaacaaaacc ctgtcttaca ggtggatggc
     1081 ctcacttcgt gaaaccatcc accccgcata agttttttaa tcggtggtgt cgtcaatcac
     1141 taaatcggaa ccccaaaggg gacccccacg atttagcatt tgacgagatt aactctcgga
     1201 gatggcatca tgcttttaac ggactttaaa aaacggtgaa cggatgcgga ttttaatccg
     1261 tgtctgtgag gcgttgagca acgagcaaag cgaggcggta gaaagagagc cagcggcagg
     1321 ctcaggacga taatccgggg aaaatgtacc actcgcagag tgttaacatt tgacatcgga
     1381 ttgtctatcc tgcattggtg tcgagttggc aaggctgagg ataatgcagg atagacagac
     1441 cactagagat tgcatgcaat ctcgtaccta tcacctcagc ctgccgctgg ctcttttcca
     1501 gaactaagaa tgcgttctta aaaatccaaa cttgataccg tttaaatcta ttttcacaaa
     1561 aagagttttt accctctcgt ttgttttctg aatatataaa attaattcat cctcaaaacc
     1621 ttcgtgcgga tgaaaaccaa atgtgccaaa acttaatcca tcaataagca aaataaggtt
     1681 tttaaacctg tcaggaatca ccgcttcact tcttaaaaaa aataaaccaa tagtcttatt
     1741 tccattcagg tattcaatat taatattcat acgttaatcc gatttatcct tctcaggggc
     1801 gcgtttgtaa cgccgtttac ctgaatccca gacaaacgcc gtccgcttcc cgtcatcagt
     1861 gccatcccct acatcgtcgg caatgacaag atcctcattg gtttctcgat ccaactggag
     1921 gacgtttttt agcgccccac cggtcgagat aaaacggcgc ttgtgaagct gccgcgtcag
     1981 ctcaagaaac cactcaggat cgtttgccat atcttccggt ttaacggagt atttcagcgt
     2041 ttcagcaacc gcgctttgca gttgctcggc aacg
//
