LOCUS       NZ_CP053363             3511 bp    DNA     circular CON 23-DEC-2023
DEFINITION  Klebsiella pneumoniae strain EGKP6 plasmid pEGKP6.5, complete
            sequence.
ACCESSION   NZ_CP053363
VERSION     NZ_CP053363.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14858807
            Assembly: GCF_017161155.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Hamza,E., Dona,V., Garcia,A., Elhariri,M., Hamza,D., Elhelw,R. and
            Perreten,V.
  TITLE     Whole genome sequencing of Klebsiella pneumoniae carrying NDM-1
            isolated from Egypt
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Hamza,E., Dona,V., Garcia,A. and Perreten,V.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAY-2020) Institute of Veterinary Bacteriology,
            University of Bern, Laenggassstrasse 122, Bern 3012, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/23/2023 20:02:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,725
            CDSs (total)                      :: 5,600
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 54 of 144
            Pseudo Genes (incomplete)         :: 83 of 144
            Pseudo Genes (internal stop)      :: 29 of 144
            Pseudo Genes (multiple problems)  :: 19 of 144
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP053363.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="EGKP6"
                     /isolation_source="feces from livestock farm"
                     /host="cattle"
                     /db_xref="taxon:573"
                     /plasmid="pEGKP6.5"
                     /geo_loc_name="Egypt:Giza"
                     /collection_date="2015"
     gene            134..631
                     /locus_tag="HMI21_RS28555"
                     /old_locus_tag="HMI21_29040"
     CDS             134..631
                     /locus_tag="HMI21_RS28555"
                     /old_locus_tag="HMI21_29040"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_015328238.1"
                     /translation="MSSLLTASRDFEEKSKAQQQLTEERLKAAFSKHENVVKSELNASA
                     KRINDAISAHEQGMNAAMQSNRLSVLRMVGRTWLTITMVSGLLFASLSGVLWYQGNRIA
                     ANLAEIRQQRDTLSKLHMQTWGVTYLENRNGRFLVLPEGMKAETGRTVDNKTRNAVKLV
                     RE"
                     /name="MbeB family mobilization protein"
     gene            638..853
                     /locus_tag="HMI21_RS28560"
                     /old_locus_tag="HMI21_29045"
     CDS             638..853
                     /locus_tag="HMI21_RS28560"
                     /old_locus_tag="HMI21_29045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015328239.1"
                     /translation="MTELEKQLLSALEQLQQDYSRRLDEWESAFEEWRKMSGLMQRENA
                     ALSERVSGLSRQVQSLSEQLRRLSTG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1248..1430
                     /locus_tag="HMI21_RS28565"
                     /old_locus_tag="HMI21_29050"
     CDS             1248..1430
                     /locus_tag="HMI21_RS28565"
                     /old_locus_tag="HMI21_29050"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016265984.1"
                     /translation="MLTFIGVVVVAVIAFKLFMLFFSSSESAVERATRKYMENPTSANY
                     KIMVATRMRNEKKRG"
                     /name="hypothetical protein"
     gene            2626..2967
                     /locus_tag="HMI21_RS28570"
                     /old_locus_tag="HMI21_29060"
     CDS             2626..2967
                     /locus_tag="HMI21_RS28570"
                     /old_locus_tag="HMI21_29060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_071599364.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPTR
                     AGRLPSISPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGPDDDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          2368..2541
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          complement(1880..2401)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.69"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          2..444
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="81.081"
                     /coverage="86.0"
                     /evalue="1.13e-64"
     Typing          2648..2961
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.453"
                     /coverage="99.0"
                     /evalue="9.860000000000001e-59"
     Typing          2958..3511
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.169999999999999e-119"
ORIGIN
        1 gagctgaaaa cgcgtgatga cgtcacaggg gcgctcacag cggccggttt tgaggtggtc
       61 agaaccacaa aaaacagtat cagcattgcc gacccggacg gggggcgaaa tatccgactg
      121 aggggagcca tttatgagca gtcttttaac ggcctcgagg gacttcgagg agaaatcgaa
      181 agcacagcag cagcttaccg aagagaggct gaaagccgca ttcagcaagc acgagaacgt
      241 tgtcaaatcg gaactgaacg caagcgcgaa gagaatcaac gacgccatca gcgcccacga
      301 acaggggatg aacgcggcga tgcagtcgaa ccggctgagc gtgctgcgaa tggtcgggcg
      361 gacgtggctg accatcacca tggtgtcagg gctgctgttc gccagcctga gcggggtgct
      421 gtggtaccag gggaaccgga tagcggcgaa cctggcggaa atcaggcagc agagagatac
      481 gctgtcgaaa ctgcacatgc agacgtgggg cgtgacctat ctggagaacc gcaacggacg
      541 gtttctggtg ctgccggagg ggatgaaagc cgaaacgggc cggacggtgg acaacaagac
      601 gcggaacgcg gtgaagctgg tacgggagta gagcagaatg acggaactgg aaaaacaatt
      661 gctgagcgcg ttagagcagc tacagcagga ctactcgaga aggctggacg agtgggagag
      721 cgccttcgag gaatggcgga agatgtctgg tcttatgcaa cgggagaacg cggcgctgag
      781 cgagcgcgtg tcgggcttga gcaggcaggt gcagagtttg agcgagcagc tgcgccggtt
      841 atcgacaggc tgaatgagat tgagcaacag cgatacgggc aaacaacgga aaaggaacca
      901 gagaagacag cagatctggt accggaacgg caacgcacat tatctcatgg tgacagggag
      961 tatggagact gataaacgcc cgtctcaggt ggcctgagcg tgacggggat catggccatt
     1021 ttctgcgatt tcagcacaca gagagtcttt tttaaccgaa atcaccggct atgcagtgat
     1081 gtgaataagt gatgcatatg cgtaaaataa atcaggtgcg tttctggtgg ttttcggtgt
     1141 acttgccacc accttttgcc cgtcagccat cagaaacgcc ctgagtgcct ccggcgcggt
     1201 gcgggtaaga gatgttatgt ttaatgttga taacaggagt aagagtgatg cttacattta
     1261 ttggtgtagt ggtagtcgcc gttattgcat ttaaattatt tatgcttttt tttagttcat
     1321 ccgaatctgc cgtcgaacgt gctacccgta aatatatgga gaaccctacg agtgctaact
     1381 ataaaataat ggtggctaca cgcatgagaa atgagaagaa aagagggtga tatatgtttg
     1441 agtttattgg ttttttattt ttacttttgg tgtgttatgt ctttatacaa atggttgctg
     1501 tcagggtgtt tcctgaatat ggagtacgga aagcggagga acgataccgt cgaaatccat
     1561 cagcagaaaa tgaggatctg ttatggagag ctagaagtcg tataactaaa cgcaagtaat
     1621 gtttcctaag tgtgttgata ggcaaacatg gtgggagtta ctactctcgg agttaatgat
     1681 cttcttgtga tcctgtcgtt tcagtcgtaa tctcttgctc tgaaaacgaa aaaaccacct
     1741 ggggaggtgg tttgatcgaa ggttaagtca gttggggaac tgcttaacca ggtaactggg
     1801 tatacgagac cgcagatacc aaaactgttc tttcagtgta gccgcagtca ggcccatact
     1861 tcaagacctc tgaatctctc tctcgtatat cccagttacc agtggctgct gccagtggcg
     1921 ctttgtcgtg ctttctcggg ttggactcaa gatgatagtt accgggtaag gcgcagcagt
     1981 cggactgaac ggggggtttg tgcatacagt ccagcttgga gcgaactgcc tacccggaac
     2041 tgagtgtcag gcgtggaatg agataaacgc ggccataaca gcggaatgac accggtaaac
     2101 cgaaaggcag gaacaggaga gcgcacgagg gagccgccag ggggaaacgc ctggtatctt
     2161 tatagtcctg tcgggtttcg ccaccactga tttgagcgtc agatttcgtg atgcttgtca
     2221 ggggggcgga gcctatggaa aaacggcttc gccgcggccc tatcgcttcc ctgttaagta
     2281 tcttcctggc atcttccagg aaatctccgc cccgttcgta agccatttcc gctcgccgca
     2341 gtcgaacgac cgagcgtagc gagtcagtga gcgaggaagc ggaatatatc ctgtatcaca
     2401 tattctgctg acgcgccggt gccgcctttt ttctcctgcc acatgaagca cttcactgac
     2461 atcctcatcc gtgccaacat agtaagccag tatacactcc gctagcgcta cgtgactggt
     2521 tcagggctgc gccccgaaac cccactgacg cgcctgcggc ttgtccaacc ccgcgccgac
     2581 cgggaaaggc tttcccgtcc gtcacggcgt tatcaggagg cgattctggc agataaacgc
     2641 aacaagatgc tcacgatgtg ggtgacggaa gacgaacacc ggcggttgct tgagcggtgc
     2701 gacggaaaac aactggcggc gtggatgcgc cagacctgcc ttgatgagaa accgacacgc
     2761 gccggcaggc ttccctcgat ttcgcccgcg ctgctgcgtc agcttgccgg catgggcaat
     2821 aacctgaacc agatcgcccg gaaagtgaac gccggtggtg ccggtcatga ccgggtgcag
     2881 atcgtggcag cgctgatggc gatcgatgcc ggtctggaac ggttgcggca tgccgtactg
     2941 gaaaaagggc cggacgatga ttgttaaatt ccatccccgg ggtcgcggcg ggggcgccgg
     3001 tcctgtggat tatcttctgg gtaaagaccg gcagcgtgaa ggtgccagcg tcctgcaggg
     3061 gaagccggaa gaagtccggg agctgattga tgcgtcgccg tatgtgaaaa aatatacctc
     3121 cggggtgctg tcattcgcgg aggcggacct gccgccgggt cagcgtgaaa aactgatggc
     3181 cagctttgag cgggttctga tgcccggact cgataaagac cagtacagca ttctgtgggt
     3241 ggaacacgca gacaagggtc gtcttgagct gaattttctg atccccaata ccgaattact
     3301 gacggggaaa cgcctccagc cgtattacga tcgtgccgac cggccgcgca tcgatgcgtg
     3361 gcagacagtg gtcaacggca ggctggggct acatgatccg aacgacccgg agaaccggcg
     3421 ggcgctggtc acgccttcag gactgccaga aacgaagcag gaagccgcag agacgattac
     3481 acggggtttg ctgatgttag cctcatcagg a
//
